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DraconiticBiochemistryBiomolecules · Catabolism · Biosynthesis · MetabolismMap
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Arachidonate metabolism

Liberation and oxygenation of arachidonate into prostanoid, leukotriene and related mediator branches. 88 reactions, from Reactome R-HSA-2142753.

  1. PLA2G4A (cPLA2) hydrolyzes phosphatidylcholineH2O + AA-PC → AA + H+ + LysoPtdCho
  2. Arachidonate is oxidised to PGG2 by PTGS12 O2 + AA → PGG2
  3. PGG2 is reduced to PGH2 by PTGS1PGG2 + hydrogen donor → H2O + PGH2 + hydrogen acceptor
  4. CYP4F2, 4F3 20-hydroxylate LTB4LTB4 + NADPH + H+ + O2 → 20OH-LTB4 + H2O + NADP+
  5. PGH2 is reduced to PGF2a by AKR1C3PGH2 + NADPH + H+ → PGF2a + NADP+
  6. PGJ2 is isomerised to delta12-PGJ2PGJ2 → delta12-PGJ2
  7. LTB4 is oxidised to 12-oxoLTB4 by PTGR1LTB4 + NADP+ → 12-oxoLTB4 + NADPH + H+
  8. Delta12-PGJ2 is dehydrated to 15d-PGJ2delta12-PGJ2 → 15d-PGJ2 + H2O
  9. PGH2 is reduced to PGF2a by FAM213BPGH2 + TXN-S2H2 → PGF2a + TXN-S2
  10. PGH2 is degraded to 12S-HHT and MDA by TBXAS1PGH2 → 12S-HHT + MDA
  11. PGD2 is reduced to 11-epi-PGF2a by AKRIC3PGD2 + H+ + NADPH → 11epi-PGF2a + NADP+
  12. PGI2 is hydrolysed to 6k-PGF1aPGI2 + H2O → 6k-PGF1a
  13. PGH2 is isomerised to PGD2 by PTGDSPGH2 → PGD2
  14. PGE2 is converted to PGF2a by CBR1PGE2 + NADPH + H+ → PGF2a + NADP+
  15. PGE2 is dehydrated to PGA2PGE2 → H2O + PGA2
  16. PGH2 is isomerised to PGE2 by PTGESPGH2 → PGE2
  17. PGD2/E2/F2a is oxidised to 15k-PGD2/E2/F2a by HPGDPGD2/E2/F2a + NAD+ → 15k-PGD2,15k-PGE2,15k-PGF2a + NADH + H+
  18. PGA2 is isomerised to PGC2PGA2 → PGC2
  19. PGA2 is dehydrated to 15d-PGA2PGA2 → 15d-PGA2 + H2O
  20. PGD2 is dehydrated to 15d-PGD2PGD2 → 15d-PGD2 + H2O
  21. 15k-PGE2/F2a is reduced to dhk-PGE2/F2a by PTGR115k-PGE2,15k-PGF2a + NADPH + H+ → dhk-PGE2,dhk-PGF2a + NADP+
  22. PGH2 is isomerised to PGD2 by HPGDSPGH2 → PGD2
  23. TXB2 is converted to 11dh-TXB2 by TXDHTXB2 + NAD+ → 11dh-TXB2 + NADH + H+
  24. PGD2 is dehydrated to PGJ2PGD2 → PGJ2 + H2O
  25. PGC2 is isomerised to PGB2PGC2 → PGB2
  26. 20oh-LTB4 is oxidised to 20cho-LTB4 by CYP4F2/4F320OH-LTB4 + NADPH + H+ + O2 → 20cho-LTB4 + NADP+ + 2 H2O
  27. EXA4 is converted to EXC4 by LTC4SEXA4 + GSH → EXC4
  28. 5S-HETE is oxidised to 5-oxoETE by 5-HEDH5S-HETE + NADP+ → 5-oxoETE + NADPH + H+
  29. 15S-HETE is oxidised to 15-oxoETE by 15-HEDH15S-HETE + NAD(P)+ → 15-oxoETE + NAD(P)H + H+
  30. 20cooh-LTB4 is converted to 18cooh-LTB420cooh-LTB4 → 18cooh-LTB4
  31. 15S-HpETE is reduced to 15S-HETE by GPX1/2/415S-HpETE + 2 GSH → 15S-HETE + GSSG + H2O
  32. 20cho-LTB4 is oxidised to 20cooh-LTB4 by CYP4F2/4F320cho-LTB4 + NADPH + H+ + O2 → 20cooh-LTB4 + NADP+ + H2O
  33. Arachidonate is converted to HXA3/B3 by ALOX12AA + O2 → HXA3/B3
  34. Arachidonate is hydroxylated to 16-HETE by CYP(1)AA + O2 + NADPH + H+ → NADP+ + H2O + 16-HETE
  35. Arachidonate is hydroxylated to 19-HETE by CYP(2)AA + O2 + NADPH + H+ → 19-HETE + NADP+ + H2O
  36. EXD4 is converted to EXE4 by DPEPEXD4 + H2O → EXE4 + Gly
  37. Arachidonate is epoxidated to 5,6-EET by CYP(4)AA + O2 + NADPH + H+ → 5,6-EET + NADP+ + H2O
  38. Arachidonate is epoxidated to 8,9-EET by CYP(5)AA + O2 + NADPH + H+ → NADP+ + H2O + 8,9-EET
  39. Arachidonate is hydroxylated to 20-HETE by CYP(3)AA + O2 + NADPH + H+ → 20-HETE + NADP+ + H2O
  40. EXC4 is converted to EXD4 by GGTEXC4 → EXD4 + L-Glu
  41. 5S-HpETE is reduced to 5S-HETE by GPX1/2/45S-HpETE + 2 GSH → 5S-HETE + GSSG + H2O
  42. Arachidonate is converted to 12-oxoETE by ALOX12AA → 12-oxoETE
  43. HXA3/B3 is hydrolysed to TrXA3/B3 by HXEHHXA3/B3 + H2O → TrXA3/B3
  44. Arachidonate is oxidised to 12R-HpETE by ALOX12BAA + O2 → 12R-HpETE
  45. Arachidonate is oxidised to 15R-HETE by Acetyl-PTGS2AA + O2 + NADPH + H+ → 15R-HETE + NADP+ + H2O
  46. 12R-HpETE is reduced to 12R-HETE by GPX1/2/412R-HpETE + 2 GSH → 12R-HETE + GSSG + H2O
  47. 5,6-EET is hydrolysed to 5,6-DHET by EPHX2H2O + 5,6-EET → 5,6-DHET
  48. LTA4 is hydrolysed to 6t-/6t,12epi-LTB4LTA4 + H2O → 6t/6t,12epi-LTB4
  49. Arachidonate is oxidised to 12S-HpETE by ALOX12/15AA + O2 → 12S-HpETE
  50. 20cho-LTB4 is oxidised to 20cooh-LTB4 by ALDH20cho-LTB4 + NADPH + H+ + O2 → 20cooh-LTB4 + NADP+ + H2O
  51. 12S-HpETE is reduced to 12S-HETE by GPX1/2/412S-HpETE + 2 GSH → 12S-HETE + GSSG + H2O
  52. Arachidonate is oxidised to 15S-HpETE by ALOX15/15BAA + O2 → 15S-HpETE
  53. LTA4 is converted to EXA4 by ALOX15LTA4 → EXA4
  54. PGH2 diffuses from the endoplasmic reticulum lumen to the cytosolPGH2 → PGH2
  55. PGG2 is reduced to PGH2 by PTGS2PGG2 + hydrogen donor → H2O + PGH2 + hydrogen acceptor
  56. PTGS2 dimer binds PTGS2 inhibitorsPTGS2 dimer + PTGS2 inhibitors → PTGS2 dimer:PTGS2 inhibitors
  57. Arachidonate is oxidised to PGG2 by PTGS22 O2 + AA → PGG2
  58. ASA- acetylates PTGS1PTGS1 dimer + ASA- → Ac-PTGS1 dimer + ST
  59. ASA- acetylates PTGS2PTGS2 dimer + ASA- → Ac-PTGS2 dimer + ST
  60. Prostaglandin E synthase isomerizes PGH2 to PGE2PGH2 → PGE2
  61. Arachidonate is oxidised to 5S-HpETE by ALOX5AA + O2 → 5S-HpETE
  62. LTD4 is converted to LTE4 by DPEP1/2LTD4 + H2O → LTE4 + Gly
  63. GGT1, 5 dimers hydrolyse LTC4 to LTD4LTC4 + H2O → LTD4 + L-Glu
  64. LTA4 is converted to LTC4 by LTC4SLTA4 + GSH → LTC4
  65. 5S-HpETE is dehydrated to LTA4 by ALOX55S-HpETE → LTA4 + H2O
  66. LTC4 is exported from the cytosol by ABCC1LTC4 + ATP + H2O → H+ + Pi + LTC4 + ADP
  67. LTA4 is hydolysed to LTB4 by LTA4HLTA4 + H2O → LTB4
  68. SLC27A1 transports arachidonate across the ER membraneAA → AA
  69. ALOX5 is phosphorylated by MAPKAP2ALOX5:Ca2+:Fe2+ + ATP → p-S272-ALOX5:Ca2+:Fe2+ + ADP + H+
  70. TXA2 is hydrolysed to TXB2TXA2 + H2O → TXB2
  71. FAAH hydrolyses AEA to AA and ETAAEA + H2O → AA + ETA
  72. FAAH2 hydrolyses AEA to AA and ETAAEA + H2O → AA + ETA
  73. AWAT1 transfers acyl group from acyl-CoA to ARACOH, forming wax estersARACOH + Acyl-CoA → arachidyl ester + CoA-SH
  74. PTGIS, CYP8A1 isomerise PGH2 to PGI2PGH2 → PGI2
  75. TBXAS1 isomerises PGH2 to TXA2PGH2 → TXA2
  76. PON1,2,3:Ca2+ dimers hydrolyse 5-HETEL to 5-HETE5-HETEL + H2O → 5-HETE
  77. ALOXE3 isomerises 12R-HpETE to HXA312R-HpETE → HXA3
  78. PTGS1 dimer binds PTGS1 InhibitorsPTGS1 dimer + PTGS1 Inhibitors → PTGS1 dimer:PTGS1 Inhibitors
  79. ALOX5 binds ALOX5 inhibitorsALOX5:ALOX5AP:LTC4S + ALOX5 inhibitors → ALOX5:ALOX5 inhibitors
  80. PTGS2 dimer binds PTGS inhibitorsPTGS2 dimer + PTGS inhibitors → PTGS2 dimer:PTGS inhibitors
  81. PTGS1,2 dimers bind PTGS InhibitorsPTGS1 dimer + PTGS inhibitors + PTGS2 dimer → PTGS1 dimer:PTGS Inhibitors + PTGS2 dimer:PTGS inhibitors
  82. 8,9-EET is hydrolysed to 8.9-DHET by EPHX2H2O + 8,9-EET → 8,9-DHET
  83. Arachidonate is epoxidated to 11,12-EET by CYP(5)AA + O2 + NADPH + H+ → NADP+ + H2O + 11,12-EET
  84. 11,12-EET is hydrolysed to 11,12-DHET by EPHX2H2O + 11,12-EET → 11,12-DHET
  85. Arachidonate is hydroxylated to 17-HETE by CYP(1)AA + O2 + NADPH + H+ → NADP+ + H2O + 17-HETE
  86. Arachidonate is hydroxylated to 18-HETE by CYP(1)AA + O2 + NADPH + H+ → NADP+ + H2O + 18-HETE
  87. 14,15-EET is hydrolysed to 14,15-DHET by EPHX2H2O + 14,15-EET → 14,15-DHET
  88. Arachidonate is epoxidated to 14,15-EET by CYP(5)AA + O2 + NADPH + H+ → NADP+ + H2O + 14,15-EET