Arachidonate is epoxidated to 14,15-EET by CYP(5)
AA + O2 + NADPH + H+ → NADP+ + H2O + 14,15-EET
- Reactome
- R-HSA-9915997
- Compartment
- endoplasmic reticulum membrane · cytosol
- Rhea
- RHEA:51473
Inputs
- AACHEBI:32395, C20H31O2, cytosol
- O2CHEBI:15379, O2, cytosol
- NADPHCHEBI:57783, C21H26N7O17P3, cytosol
- H+CHEBI:15378, H, cytosol
Outputs
- NADP+CHEBI:58349, C21H25N7O17P3, cytosol
- H2OCHEBI:15377, H2O, cytosol
- 14,15-EETCHEBI:84024, C20H31O3, cytosol
Catalysed by
- monooxygenase activity of CYP(5) [endoplasmic reticulum membrane]
Literature
- Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1 Drug Metab Dispos, 2004
- Analysis of cytochrome P450 metabolites of arachidonic and linoleic acids by liquid chromatography-mass spectrometry with ion trap MS Anal Biochem, 1998
- Arachidonic acid metabolism by human cytochrome P450s 2C8, 2C9, 2E1, and 1A2: regioselective oxygenation and evidence for a role for CYP2C enzymes in arachidonic acid epoxygenation in human liver microsomes Arch Biochem Biophys, 1995
- Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart J Biol Chem, 1996