CYP27B1 hydroxylates 25(OH)D to 1,25(OH)2D
25(OH)D + H+ + NADPH + O2 → 1,25(OH)2D + H2O + NADP+
- Reactome
- R-HSA-209868
- Compartment
- cytosol · mitochondrial outer membrane
- Rhea
- None recorded
Inputs
- 25(OH)DCHEBI:17933, C27H44O2, cytosol
- H+CHEBI:15378, H, cytosol
- NADPHCHEBI:57783, C21H26N7O17P3, cytosol
- O2CHEBI:15379, O2, cytosol
Outputs
- 1,25(OH)2DCHEBI:17823, C27H44O3, cytosol
- H2OCHEBI:15377, H2O, cytosol
- NADP+CHEBI:58349, C21H25N7O17P3, cytosol
Catalysed by
- calcidiol 1-monooxygenase activity of CYP27B1(?-508) [mitochondrial outer membrane]EC 1.14.15.18; UniProt O15528
Literature
- Synthesis of 1,25-dihydroxyvitamin D(3) by human endothelial cells is regulated by inflammatory cytokines: a novel autocrine determinant of vascular cell adhesion J Am Soc Nephrol, 2002
- Regulation of 25-hydroxyvitamin D3-1 alpha-hydroxylase and production of 1 alpha,25-dihydroxyvitamin D3 by human dendritic cells Blood, 2003
- Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli Eur. J. Biochem., 1999