CYP11A1 cleaves 20a,22b-DHCHOL
20a,22b-DHCHOL + O2 + 2 CYP11A1:FDXR:FDX1,FDX1L (red.) + 2 NADPH + 2 H+ → PREG + ISCAL + 2 H2O + 2 CYP11A1:FDXR:FDX1,FDX1L (ox.) + 2 NADP+
- Reactome
- R-HSA-193101
- Compartment
- mitochondrial matrix · mitochondrial inner membrane
- Rhea
- RHEA:34344
Inputs
- 20a,22b-DHCHOLCHEBI:1294, C27H46O3, mitochondrial inner membrane
- O2CHEBI:15379, O2, mitochondrial matrix
- 2 CYP11A1:FDXR:FDX1,FDX1L (red.)R-HSA-5580249, mitochondrial matrix
- 2 NADPHCHEBI:57783, C21H26N7O17P3, mitochondrial matrix
- 2 H+CHEBI:15378, H, mitochondrial matrix
Outputs
- PREGCHEBI:16581, C21H32O2, mitochondrial matrix
- ISCALCHEBI:17998, C6H12O, mitochondrial matrix
- 2 H2OCHEBI:15377, H2O, mitochondrial matrix
- 2 CYP11A1:FDXR:FDX1,FDX1L (ox.)R-HSA-5580259, mitochondrial matrix
- 2 NADP+CHEBI:58349, C21H25N7O17P3, mitochondrial matrix
Catalysed by
- cholesterol monooxygenase (side-chain-cleaving) activity of CYP11A1:FDXR:FDX1,FDX1L (red.) [mitochondrial matrix]EC 1.14.15.6
Literature
- Expression of catalytically active human cytochrome p450scc in Escherichia coli and mutagenesis of isoleucine-462 Arch Biochem Biophys, 1998
- Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta Proc Natl Acad Sci U S A, 1986
- Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system Proc. Natl. Acad. Sci. U.S.A., 2011