TMP, uridine 2', 3', or 5' monophosphates, or deoxyuridine 3' or 5' monophosphates + H2O => thymidine or (deoxy)uridine + orthophosphate [NT5C]

TMP, U2MP, U3MP, UMP, dU3MP, dUMP + H2O → Thy-Rib, (d)U + Pi

Reactome

R-HSA-109480

Compartment

cytosol

Rhea

RHEA:11081, RHEA:37356, RHEA:27891, RHEA:29360, RHEA:29356

Catalysed by

• 5'-nucleotidase activity of NT5C dimer [cytosol]EC 3.1.3.5

Literature

1. Cytosolic high Km 5'-nucleotidase and 5'(3')-deoxyribonucleotidase in substrate cycles involved in nucleotide metabolism J Biol Chem, 2001
2. Cytoplasmic 5'(3')-nucleotidase from human placenta J Biol Chem, 1990
3. Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells J Biol Chem, 2000