Source assertion

ChEMBL direct single-protein activity record with source-reported and source-standardized lexical values preserved.

Evidence ID: ACTIVITY:ChEMBL_37:2949458
Subject dossier: ca13
Source chain: uniprot + chembl
Upstream record: 2949458
Corpus snapshot: 2026-07-14T09:23:31Z
Admission status: In this release

Lossless activity measurement

Source-reported value: = 32.0 nM
Source-standardized value: = 32.0 nM
Reported endpoint: Ki
Standardized endpoint: Ki
Reported upper bound: not reported by the source
Reported text value: not reported by the source
Standardization flag: 1
pChEMBL from source: 7.50

Reported and source-standardized fields are displayed separately and retain their exact lexical values. The standardized field does not overwrite the upstream report.

Not comparable with other recordsCONDITION_MISSING

No cross-assay comparison is authorized by this record. Evaluate target, construct, endpoint, relation, units, assay format, conditions, and exact molecular form first.

Assay and target

ChEMBL activity: 2949458
Target: CHEMBL3912, SINGLE PROTEIN
Assay: CHEMBL1048396, Binding (B)
BAO format: BAO_0000357, single protein format
BAO endpoint: BAO_0000192
Target confidence: 9, Direct single protein target assigned
Source quality flags: potential_duplicate=0; data_validity_comment=not reported by the source

Upstream assay description

Inhibition of human recombinant CA13 by stopped flow CO2 hydrase assay

Compound identity

Molecule: CHEMBL21, SULFANILAMIDE
Parent form: CHEMBL21; is_parent_form=true
Molecule type: Small molecule
Chirality source code: 2
Standard InChIKey: FDDDEECHVMSUSB-UHFFFAOYSA-N
Canonical SMILES: Nc1ccc(S(N)(=O)=O)cc1
Standard InChI: InChI=1S/C6H8N2O2S/c7-5-1-3-6(4-2-5)11(8,9)10/h1-4H,7H2,(H2,8,9,10)

Document

The coumarin-binding site in carbonic anhydrase accommodates structurally diverse inhibitors: the antiepileptic lacosamide as an example and lead molecule for novel classes of carbonic anhydrase inhibitors.

Temperini C, Innocenti A, Scozzafava A, Parkkila S, Supuran CT.

ChEMBL document: CHEMBL1154758
Journal / year: J Med Chem, 2010
DOI: 10.1021/jm901524f
PubMed: 20028100

Comparison state

Prequalified: No — pairwise evaluation is still required
Protein identity: Q8N1Q1, taxon 9606
Variant / construct: not reported by the source / not reported by the source
Conditions: pH not reported by the source; temperature not reported by the source °C; substrate not reported by the source; buffer not reported by the source

Snapshot provenance

Each row is a source snapshot this record was built from: when it was retrieved, its path and SHA-256 digest, and the exact request. The snapshot files stay with the build and are not served here. These rows document this record; they are not general citations.

Retrieved	Snapshot	SHA-256	Upstream request
2026-07-14T09:21:33Z	`raw/uniprot/entries/Q8N1Q1.json`	`606f7712a41abd05d3b9d0522bbefd377f44b400f37aba7cab2c788361ea41dd`	Open exact source
2026-07-14T09:22:20Z	`raw/chembl/targets/CHEMBL3912.json`	`25c5e2a15ee4b3b77c80ce3f132ac5e1bb083da98ac008a4a0af7dcac97dc1b5`	Open exact source
2026-07-14T09:22:47Z	`raw/chembl/direct-assays/CHEMBL3912.json`	`1b69e1b7672728589a0f96e68c5abf7840e50b3d7b09f9d1ef52048e47d6afba`	Open exact source
2026-07-14T09:23:09Z	`raw/chembl/assay-activities/CHEMBL1048396.json`	`37acd5950d0b566313be93b55ee97131f6e46504831d707440aaaae34d8bf259`	Open exact source
2026-07-14T09:23:29Z	`raw/chembl/activities/2949458.json`	`b8c42074b4b2bbe47b42126f5e0d20e07c596c530044d226f5dbddd352c2c779`	Open exact source
2026-07-14T09:23:29Z	`raw/chembl/assays/CHEMBL1048396.json`	`391d504e1319ef9240f856142a24e3b6a725d4988f2faa0e4d172f939988555a`	Open exact source
2026-07-14T09:23:29Z	`raw/chembl/molecules/CHEMBL21.json`	`cae58cda448760e0725e0bed4535e45e4648e5d6b843abc403ba5cab2a07f7b5`	Open exact source
2026-07-14T09:23:29Z	`raw/chembl/documents/CHEMBL1154758.json`	`327d7f0bb5d41473575b71055fc59ad0063774d8f21a305295f817d0924c9a6c`	Open exact source