Source assertion

ChEMBL direct single-protein activity record with source-reported and source-standardized lexical values preserved.

Evidence ID: ACTIVITY:ChEMBL_37:2456870
Subject dossier: ca12
Source chain: uniprot + chembl
Upstream record: 2456870
Corpus snapshot: 2026-07-14T09:23:31Z
Admission status: In this release

Lossless activity measurement

Source-reported value: = 7.79 uM
Source-standardized value: = 7.79 uM
Reported endpoint: Kinact
Standardized endpoint: Kinact
Reported upper bound: not reported by the source
Reported text value: not reported by the source
Standardization flag: 0
pChEMBL from source: not reported by the source

Reported and source-standardized fields are displayed separately and retain their exact lexical values. The standardized field does not overwrite the upstream report.

Not comparable with other recordsCONDITION_MISSING

No cross-assay comparison is authorized by this record. Evaluate target, construct, endpoint, relation, units, assay format, conditions, and exact molecular form first.

Assay and target

ChEMBL activity: 2456870
Target: CHEMBL3242, SINGLE PROTEIN
Assay: CHEMBL1022730, Binding (B)
BAO format: BAO_0000357, single protein format
BAO endpoint: BAO_0000179
Target confidence: 9, Direct single protein target assigned
Source quality flags: potential_duplicate=0; data_validity_comment=not reported by the source

Upstream assay description

Inhibition of human recombinant carbonic anhydrase 12 catalytic domain by stopped flow CO2 hydration method

Compound identity

Molecule: CHEMBL442687, TIOXOLONE
Parent form: CHEMBL442687; is_parent_form=true
Molecule type: Small molecule
Chirality source code: 2
Standard InChIKey: SLYPOVJCSQHITR-UHFFFAOYSA-N
Canonical SMILES: O=c1oc2cc(O)ccc2s1
Standard InChI: InChI=1S/C7H4O3S/c8-4-1-2-6-5(3-4)10-7(9)11-6/h1-3,8H

Document

Carbonic anhydrase inhibitors: thioxolone versus sulfonamides for obtaining isozyme-selective inhibitors?

Innocenti A, Maresca A, Scozzafava A, Supuran CT.

ChEMBL document: CHEMBL1155067
Journal / year: Bioorg Med Chem Lett, 2008
DOI: 10.1016/j.bmcl.2008.06.024
PubMed: 18572406

Comparison state

Prequalified: No — pairwise evaluation is still required
Protein identity: O43570, taxon 9606
Variant / construct: not reported by the source / not reported by the source
Conditions: pH not reported by the source; temperature not reported by the source °C; substrate not reported by the source; buffer not reported by the source

Snapshot provenance

Each row is a source snapshot this record was built from: when it was retrieved, its path and SHA-256 digest, and the exact request. The snapshot files stay with the build and are not served here. These rows document this record; they are not general citations.

Retrieved	Snapshot	SHA-256	Upstream request
2026-07-14T09:21:33Z	`raw/uniprot/entries/O43570.json`	`5e3bf6d20c857bc7f533ebfbfaf42d7ad6e84050aeb13c8f959567b94eb24d19`	Open exact source
2026-07-14T09:22:20Z	`raw/chembl/targets/CHEMBL3242.json`	`755d3035fb62c182deaa42ea303e1e55fc0fe5fe75345c43ebe9660f9437e5d2`	Open exact source
2026-07-14T09:22:45Z	`raw/chembl/direct-assays/CHEMBL3242.json`	`bda50d938bc0a6b306883bf29ecfc51ea24cc7767b8168612503cbcaed842608`	Open exact source
2026-07-14T09:23:09Z	`raw/chembl/assay-activities/CHEMBL1022730.json`	`aee24b9186fb63bd1a38d3afa519c0b97cc79d586fbf79a82daf1e8447b45538`	Open exact source
2026-07-14T09:23:28Z	`raw/chembl/activities/2456870.json`	`739e42bfc418542ab874d081b0157652d92332ef558306eea14c8e462d34b7af`	Open exact source
2026-07-14T09:23:28Z	`raw/chembl/assays/CHEMBL1022730.json`	`85a771df7d36382ee7ebce7da4049b5a52d78b91d415b428855df664cbb88309`	Open exact source
2026-07-14T09:23:19Z	`raw/chembl/molecules/CHEMBL442687.json`	`f152b44651db17cb570a3d901bba045a331eebd01e4fac702cecfbf64650aa99`	Open exact source
2026-07-14T09:23:19Z	`raw/chembl/documents/CHEMBL1155067.json`	`a852cdb1b30571a0f6ed5def67b2c61a1b73e56c6483dd07330b61bc3a77e725`	Open exact source