Source assertion

ChEMBL direct single-protein activity record with source-reported and source-standardized lexical values preserved.

Evidence ID: ACTIVITY:ChEMBL_37:2250659
Subject dossier: ca3
Source chain: uniprot + chembl
Upstream record: 2250659
Corpus snapshot: 2026-07-14T09:23:31Z
Admission status: In this release

Lossless activity measurement

Source-reported value: = 2.0 10'5nM
Source-standardized value: = 2.0 10'5nM
Reported endpoint: Kinact
Standardized endpoint: Kinact
Reported upper bound: not reported by the source
Reported text value: not reported by the source
Standardization flag: 0
pChEMBL from source: not reported by the source

Reported and source-standardized fields are displayed separately and retain their exact lexical values. The standardized field does not overwrite the upstream report.

Not comparable with other recordsCONDITION_MISSING, UNSUPPORTED_UNIT

No cross-assay comparison is authorized by this record. Evaluate target, construct, endpoint, relation, units, assay format, conditions, and exact molecular form first.

Assay and target

ChEMBL activity: 2250659
Target: CHEMBL2885, SINGLE PROTEIN
Assay: CHEMBL1019861, Binding (B)
BAO format: BAO_0000357, single protein format
BAO endpoint: BAO_0000179
Target confidence: 9, Direct single protein target assigned
Source quality flags: potential_duplicate=0; data_validity_comment=not reported by the source

Upstream assay description

Inhibition of human recombinant CA3 by stopped flow CO2 hydration assay

Compound identity

Molecule: CHEMBL20, ACETAZOLAMIDE
Parent form: CHEMBL20; is_parent_form=true
Molecule type: Small molecule
Chirality source code: 2
Standard InChIKey: BZKPWHYZMXOIDC-UHFFFAOYSA-N
Canonical SMILES: CC(=O)Nc1nnc(S(N)(=O)=O)s1
Standard InChI: InChI=1S/C4H6N4O3S2/c1-2(9)6-3-7-8-4(12-3)13(5,10)11/h1H3,(H2,5,10,11)(H,6,7,9)

Document

Carbonic anhydrase inhibitors. Comparison of chlorthalidone and indapamide X-ray crystal structures in adducts with isozyme II: when three water molecules and the keto-enol tautomerism make the difference.

Temperini C, Cecchi A, Scozzafava A, Supuran CT.

ChEMBL document: CHEMBL1139383
Journal / year: J Med Chem, 2009
DOI: 10.1021/jm801386n
PubMed: 19115843

Comparison state

Prequalified: No — pairwise evaluation is still required
Protein identity: P07451, taxon 9606
Variant / construct: not reported by the source / not reported by the source
Conditions: pH not reported by the source; temperature not reported by the source °C; substrate not reported by the source; buffer not reported by the source

Snapshot provenance

Each row is a source snapshot this record was built from: when it was retrieved, its path and SHA-256 digest, and the exact request. The snapshot files stay with the build and are not served here. These rows document this record; they are not general citations.

Retrieved	Snapshot	SHA-256	Upstream request
2026-07-14T09:21:32Z	`raw/uniprot/entries/P07451.json`	`cf4d0cf96a6252efc608b8ee5c8b35b502dffa8996e6dbb74c6fa4b67ce3394f`	Open exact source
2026-07-14T09:22:19Z	`raw/chembl/targets/CHEMBL2885.json`	`dcbc37484e42848b7fca675d4e4820ee510fa2c8a5c36fdfeb104caaef140929`	Open exact source
2026-07-14T09:22:26Z	`raw/chembl/direct-assays/CHEMBL2885.json`	`1cec96f9a90a32ed8fc3be670fe9b64fc53f889877345862e36222d5a11be511`	Open exact source
2026-07-14T09:22:52Z	`raw/chembl/assay-activities/CHEMBL1019861.json`	`74ae8dbe77f43df359c3102aa3e69a0410751d93419a0621cedd6559589b4d1c`	Open exact source
2026-07-14T09:23:19Z	`raw/chembl/activities/2250659.json`	`3d81291f3b40e74aa66733060dd547e67b08d30293a7d4536a20a5884d2053fb`	Open exact source
2026-07-14T09:23:20Z	`raw/chembl/assays/CHEMBL1019861.json`	`c835fe3879e941e73cd7fa5be219c03b5e75a1e3bd3b05d5442d340b566bda06`	Open exact source
2026-07-14T09:23:20Z	`raw/chembl/molecules/CHEMBL20.json`	`6b07a8754aad1e0e3a2ad71a185215a21d0c942143f77e80555dd6d7fe39df19`	Open exact source
2026-07-14T09:23:20Z	`raw/chembl/documents/CHEMBL1139383.json`	`ed83ab3f991235315310bfa93368979a438a3961fb71a211ed7d627c5da71fd5`	Open exact source