CYP51A1 14-demethylates 24,25-dhLAN to 4,4-diMeCholesta-8,14-dien-3-ol
24,25-dhLAN + 3 CYP450:Fe2+ + 3 O2 → 4,4-diMe-8,14-cholestadien-3b-ol + HCOOH + 3 CYP450:Fe3+ + 4 H+ + 4 H2O
- Reactome
- R-HSA-9947148
- Compartment
- endoplasmic reticulum membrane · cytosol
- Rhea
- RHEA:45961
Inputs
- 24,25-dhLANCHEBI:28113, C30H52O, endoplasmic reticulum membrane
- 3 CYP450:Fe2+R-HSA-8957354, endoplasmic reticulum membrane
- 3 O2CHEBI:15379, O2, cytosol
Outputs
- 4,4-diMe-8,14-cholestadien-3b-olCHEBI:78904, C29H48O, endoplasmic reticulum membrane
- HCOOHCHEBI:15740, CHO2, cytosol
- 3 CYP450:Fe3+R-HSA-8957351, endoplasmic reticulum membrane
- 4 H+CHEBI:15378, H, cytosol
- 4 H2OCHEBI:15377, H2O, cytosol
Catalysed by
- sterol 14-demethylase activity of CYP51A1 [endoplasmic reticulum membrane]EC 1.14.14.154; UniProt Q16850
Literature
- Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species J Biochem, 1996
- Purification of a human cytochrome P-450 isozyme catalyzing lanosterol 14 alpha-demethylation Biochim Biophys Acta, 1993
- The ubiquitously expressed human CYP51 encodes lanosterol 14 alpha-demethylase, a cytochrome P450 whose expression is regulated by oxysterols Arch Biochem Biophys, 1996