ME2 tetramer decarboxylates MAL to PYR
MAL + NAD+ → PYR + CO2 + NADH
- Reactome
- R-HSA-9012268
- Compartment
- mitochondrial matrix
- Rhea
- RHEA:12654
Inputs
- MALCHEBI:15589, C4H4O5, mitochondrial matrix
- NAD+CHEBI:57540, C21H26N7O14P2, mitochondrial matrix
Outputs
- PYRCHEBI:15361, C3H3O3, mitochondrial matrix
- CO2CHEBI:16526, CO2, mitochondrial matrix
- NADHCHEBI:57945, C21H27N7O14P2, mitochondrial matrix
Catalysed by
- malate dehydrogenase (decarboxylating) (NAD+) activity of ME2:Mg2+ tetramer [mitochondrial matrix]EC 1.1.1.39
Literature
- Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli J. Biol. Chem., 1991
- Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism Structure, 2003
- Structure and function of malic enzymes, a new class of oxidative decarboxylases Biochemistry, 2003
- Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme PLoS ONE, 2012
- Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate Structure, 2002
- Human mitochondrial NAD(P)(+)-dependent malic enzyme participates in cutaneous melanoma progression and invasion J. Invest. Dermatol., 2015