POR reduces CYP450:Fe3+ to CYP450:Fe2+
NADPH + 2 CYP450:Fe3+ → NADP+ + 2 CYP450:Fe2+ + H+
- Reactome
- R-HSA-76494
- Compartment
- endoplasmic reticulum membrane · cytosol
- Rhea
- RHEA:24041
Inputs
- NADPHCHEBI:57783, C21H26N7O17P3, cytosol
- 2 CYP450:Fe3+R-HSA-8957351, endoplasmic reticulum membrane
Outputs
- NADP+CHEBI:58349, C21H25N7O17P3, cytosol
- 2 CYP450:Fe2+R-HSA-8957354, endoplasmic reticulum membrane
- H+CHEBI:15378, H, cytosol
Catalysed by
- NADPH-hemoprotein reductase activity of POR [endoplasmic reticulum membrane]EC 1.6.2.4; UniProt P16435
Literature
- Modification of the nucleotide cofactor-binding site of cytochrome P-450 reductase to enhance turnover with NADH in Vivo J. Biol. Chem., 2002
- Role of NADPH-cytochrome P450 reductase and cytochrome-b5/NADH-b5 reductase in variability of CYP3A activity in human liver microsomes Drug Metab. Dispos., 2009
- Mechanisms of Cytochrome P450-Catalyzed Oxidations ACS Catal, 2018
- Resolution of the cytochrome P-450-containing omega-hydroxylation system of liver microsomes into three components J Biol Chem, 1969
- NADPH P450 oxidoreductase: structure, function, and pathology of diseases Pharmacol Ther, 2013
- Quantification of cytochrome P450 reductase gene expression in human tissues Arch. Biochem. Biophys., 1992