UMPS dimer transfers phosphoribosyl group to ORO to form OMP

ORO + PRPP → OMP + PPi

Reactome

R-HSA-73567

Compartment

cytosol

Rhea

RHEA:10382

Catalysed by

• orotate phosphoribosyltransferase activity of UMPS dimer [cytosol]EC 2.4.2.10

Literature

1. Molecular cloning of the human UMP synthase gene and characterization of point mutations in two hereditary orotic aciduria families Am J Hum Genet, 1997
2. Intrinsic activity and stability of bifunctional human UMP synthase and its two separate catalytic domains, orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase J Biol Chem, 1996
3. Isolation and initial characterization of the single polypeptide that synthesizes uridine 5'-monophosphate from orotate in Ehrlich ascites carcinoma. Purification by tandem affinity chromatography of uridine-5'-monophosphate synthase Biochemistry, 1980
4. Structures of the human orotidine-5'-monophosphate decarboxylase support a covalent mechanism and provide a framework for drug design Structure, 2008