Pregnenolone is dehydrogenated to form pregn-5-ene-3,20-dione
PREG + NAD+ → pregn-5-ene-3,20-dione + NADH + H+
- Reactome
- R-HSA-196350
- Compartment
- cytosol · endoplasmic reticulum membrane
- Rhea
- RHEA:43925
Inputs
- PREGCHEBI:16581, C21H32O2, cytosol
- NAD+CHEBI:57540, C21H26N7O14P2, cytosol
Outputs
- pregn-5-ene-3,20-dioneCHEBI:63837, C21H30O2, cytosol
- NADHCHEBI:57945, C21H27N7O14P2, cytosol
- H+CHEBI:15378, H, cytosol
Catalysed by
- 3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity of HSD3B1 dimer, HSD3B2 dimer [endoplasmic reticulum membrane]EC 1.1.1.145
Literature
- Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells J Biol Chem, 1990
- Human placental 3 beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5----4-ene-isomerase: purification from mitochondria and kinetic profiles, biophysical characterization of the purified mitochondrial and microsomal enzymes J Steroid Biochem, 1989
- Structure and expression of a new complementary DNA encoding the almost exclusive 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase in human adrenals and gonads Mol Endocrinol, 1991
- Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase J Biol Chem, 2003