3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA)
3alpha,7alpha-dihydroxy-5beta-cholestan-26-al + NADPH + H+ + O2 → DHCA + NADP+ + H2O
- Reactome
- R-HSA-193460
- Compartment
- mitochondrial matrix
- Rhea
- None recorded
Inputs
- 3alpha,7alpha-dihydroxy-5beta-cholestan-26-alCHEBI:27428, C27H46O3, mitochondrial matrix
- NADPHCHEBI:57783, C21H26N7O17P3, mitochondrial matrix
- H+CHEBI:15378, H, mitochondrial matrix
- O2CHEBI:15379, O2, mitochondrial matrix
Outputs
- DHCACHEBI:16577, C27H46O4, mitochondrial matrix
- NADP+CHEBI:58349, C21H25N7O17P3, mitochondrial matrix
- H2OCHEBI:15377, H2O, mitochondrial matrix
Catalysed by
- oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]EC 1.6.1; UniProt Q02318
Literature
- Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis. J Biol Chem, 1991
- Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways J Biol Chem, 1998