Oxidation of 22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol
22b-HCHOL + NADPH + O2 + H+ → 20a,22b-DHCHOL + H2O + NADP+
- Reactome
- R-HSA-193065
- Compartment
- mitochondrial matrix · mitochondrial inner membrane
- Rhea
- None recorded
Inputs
- 22b-HCHOLCHEBI:1301, C27H46O2, mitochondrial inner membrane
- NADPHCHEBI:57783, C21H26N7O17P3, mitochondrial matrix
- O2CHEBI:15379, O2, mitochondrial matrix
- H+CHEBI:15378, H, mitochondrial matrix
Outputs
- 20a,22b-DHCHOLCHEBI:1294, C27H46O3, mitochondrial inner membrane
- H2OCHEBI:15377, H2O, mitochondrial matrix
- NADP+CHEBI:58349, C21H25N7O17P3, mitochondrial matrix
Catalysed by
- cholesterol monooxygenase (side-chain-cleaving) activity of CYP11A1:FDXR:FDX1,FDX1L (red.) [mitochondrial matrix]EC 1.14.15.6
Literature
- Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta Proc Natl Acad Sci U S A, 1986
- Expression of catalytically active human cytochrome p450scc in Escherichia coli and mutagenesis of isoleucine-462 Arch Biochem Biophys, 1998