PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the Golgi membrane

ATP + PI3P → ADP + PI(3,5)P2

Reactome

R-HSA-1675921

Compartment

Golgi membrane · cytosol

Rhea

RHEA:13609

Catalysed by

• 1-phosphatidylinositol-3-phosphate 5-kinase activity of PIKFYVE:VAC14:FIG4 [Golgi membrane]EC 2.7.1.150

Literature

1. PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin J Biol Chem, 1999
2. Complementation analysis in PtdInsP kinase-deficient yeast mutants demonstrates that Schizosaccharomyces pombe and murine Fab1p homologues are phosphatidylinositol 3-phosphate 5-kinases J Biol Chem, 1999
3. Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex J Biol Chem, 2007
4. ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality J Mol Biol, 2008
5. Phosphatidylinositol 3-phosphate-interacting domains in PIKfyve. Binding specificity and role in PIKfyve. Endomenbrane localization J Biol Chem, 2002