Source assertion

ChEMBL direct single-protein activity record with source-reported and source-standardized lexical values preserved.

Evidence ID: ACTIVITY:ChEMBL_37:2630964
Subject dossier: ca4
Source chain: uniprot + chembl
Upstream record: 2630964
Corpus snapshot: 2026-07-14T09:23:31Z
Admission status: In this release

Lossless activity measurement

Source-reported value: = 7.3 uM
Source-standardized value: = 7.3 uM
Reported endpoint: KA
Standardized endpoint: KA
Reported upper bound: not reported by the source
Reported text value: not reported by the source
Standardization flag: 0
pChEMBL from source: not reported by the source

Reported and source-standardized fields are displayed separately and retain their exact lexical values. The standardized field does not overwrite the upstream report.

Not comparable with other recordsCONDITION_MISSING

No cross-assay comparison is authorized by this record. Evaluate target, construct, endpoint, relation, units, assay format, conditions, and exact molecular form first.

Assay and target

ChEMBL activity: 2630964
Target: CHEMBL3729, SINGLE PROTEIN
Assay: CHEMBL1008327, Binding (B)
BAO format: BAO_0000357, single protein format
BAO endpoint: BAO_0002809
Target confidence: 9, Direct single protein target assigned
Source quality flags: potential_duplicate=0; data_validity_comment=not reported by the source

Upstream assay description

Activation of human recombinant carbonic anhydrase 4 lacking 20 amino terminal residues by stopped-flow CO2 hydrase method

Compound identity

Molecule: CHEMBL17962, HISTIDINE
Parent form: CHEMBL17962; is_parent_form=true
Molecule type: Small molecule
Chirality source code: 1
Standard InChIKey: HNDVDQJCIGZPNO-YFKPBYRVSA-N
Canonical SMILES: N[C@@H](Cc1c[nH]cn1)C(=O)O
Standard InChI: InChI=1S/C6H9N3O2/c7-5(6(10)11)1-4-2-8-3-9-4/h2-3,5H,1,7H2,(H,8,9)(H,10,11)/t5-/m0/s1

Document

Carbonic anhydrase activators. Activation of the membrane-associated isoform XV with amino acids and amines.

Innocenti A, Hilvo M, Parkkila S, Scozzafava A, Supuran CT.

ChEMBL document: CHEMBL1136977
Journal / year: Bioorg Med Chem Lett, 2009
DOI: 10.1016/j.bmcl.2009.05.026
PubMed: 19464888

Comparison state

Prequalified: No — pairwise evaluation is still required
Protein identity: P22748, taxon 9606
Variant / construct: not reported by the source / not reported by the source
Conditions: pH not reported by the source; temperature not reported by the source °C; substrate not reported by the source; buffer not reported by the source

Snapshot provenance

Each row is a source snapshot this record was built from: when it was retrieved, its path and SHA-256 digest, and the exact request. The snapshot files stay with the build and are not served here. These rows document this record; they are not general citations.

Retrieved	Snapshot	SHA-256	Upstream request
2026-07-14T09:21:32Z	`raw/uniprot/entries/P22748.json`	`28fa76c5a216b9356479d42199f709dd46e662172f2872078a5491cfe2c618f1`	Open exact source
2026-07-14T09:22:19Z	`raw/chembl/targets/CHEMBL3729.json`	`b843a8aabc22404319779ac308eef89f3cef613f26b852fa401baedf1ed2463e`	Open exact source
2026-07-14T09:22:29Z	`raw/chembl/direct-assays/CHEMBL3729.json`	`bf42f50dc047016f1cbd6f8b10da431461099db1c544514716f31370e5f30e1f`	Open exact source
2026-07-14T09:22:55Z	`raw/chembl/assay-activities/CHEMBL1008327.json`	`0543b1706960416179876d6e6717be396d28ebcee0aa2401a161a1d054cd0cee`	Open exact source
2026-07-14T09:23:20Z	`raw/chembl/activities/2630964.json`	`eae8758e3166199ab09e7efb2ef4a9dc3042ecd7c2f32932238ab0bde6ace20b`	Open exact source
2026-07-14T09:23:21Z	`raw/chembl/assays/CHEMBL1008327.json`	`964d2ead5c53c50b85630ec63e4cd9804930a7408483a5dda573192286cd3edd`	Open exact source
2026-07-14T09:23:21Z	`raw/chembl/molecules/CHEMBL17962.json`	`38c3ccb034866884e347b0be2990e332c0466e3b07d288e5249646e368efb239`	Open exact source
2026-07-14T09:23:21Z	`raw/chembl/documents/CHEMBL1136977.json`	`74d4dc8b755ae57ec9f2ceae05092dde778056f373a26904b19b1fe9d64e6e61`	Open exact source