HLCS biotinylates 6x(PCCA:PCCB)
6x(PCCA:PCCB) + 6 Btn + 6 ATP → 6x(Btn-PCCA:PCCB) + 6 PPi + 6 AMP
- Reactome
- R-HSA-2993447
- Compartment
- cytosol
- Rhea
- None recorded
Inputs
- 6x(PCCA:PCCB)R-HSA-2993809, cytosol
- 6 BtnCHEBI:15956, C10H16N2O3S, cytosol
- 6 ATPCHEBI:30616, C10H12N5O13P3, cytosol
Outputs
- 6x(Btn-PCCA:PCCB)R-HSA-3323122, cytosol
- 6 PPiCHEBI:33019, HO7P2, cytosol
- 6 AMPCHEBI:456215, C10H12N5O7P, cytosol
Catalysed by
- biotin--[biotin carboxyl-carrier protein] ligase activity of HLCS [cytosol]EC 6.3.4.10; UniProt P50747
Literature
- Mutations in the holocarboxylase synthetase gene HLCS Hum. Mutat., 2005
- Selectivity in post-translational biotin addition to five human carboxylases J. Biol. Chem., 2012
- Identification of holocarboxylase synthetase (HCS) proteins in human placenta Biochim. Biophys. Acta, 1998
- Holocarboxylase synthetase: correlation of protein localisation with biological function Arch. Biochem. Biophys., 2010