{ "schema_version": "2.0.0", "release_id": "human-metabolism-r97-expanded-20261025", "title": "Human metabolism reaction inventory", "scope": "Uncapped source-linked human metabolism across 12 domains and 58 modules.", "caveat": "The default connectivity layer shows curated reaction direction and compartment identity. 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not a duplicate of the ATP-forming human event.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-17663", "name": "Succinyl-CoA synthetase — ATP-forming", "equation": "succinyl-CoA + ADP + phosphate =\u003e succinate + ATP + CoA", "source_id": "rhea", "source_record_ids": [ "RHEA:17663" ], "source_url": "https://www.rhea-db.org/rhea/17663", "rhea_parent_id": "RHEA:17661", "human_support": [ { "source": "Reactome", "id": "R-HSA-70997", "url": "https://reactome.org/content/detail/R-HSA-70997" } ], "route": "tca", "compartment": "mitochondrial matrix", "representation": "cofactor_specific_alternative", "visual_style": "solid", "direction_note": "ATP-forming alternative; not a duplicate of the GTP-forming human event.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-13714", "name": "Succinate dehydrogenase", "equation": "a ubiquinone + succinate =\u003e a ubiquinol + fumarate", "source_id": "rhea", "source_record_ids": [ "RHEA:13714" ], "source_url": "https://www.rhea-db.org/rhea/13714", "rhea_parent_id": "RHEA:13713", "human_support": [ { "source": "Reactome", "id": "R-HSA-70994", "url": "https://reactome.org/content/detail/R-HSA-70994" } ], "route": "tca", "compartment": "mitochondrial inner membrane, matrix-facing", "representation": "single_event", "visual_style": "solid", "direction_note": "Rhea preserves a ubiquinone as a class; the human Reactome event uses Q10. The terms are not silently collapsed.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-12462", "name": "Fumarase", "equation": "fumarate + H2O =\u003e (S)-malate", "source_id": "rhea", "source_record_ids": [ "RHEA:12462" ], "source_url": "https://www.rhea-db.org/rhea/12462", "rhea_parent_id": "RHEA:12460", "human_support": [ { "source": "Reactome", "id": "R-HSA-70982", "url": "https://reactome.org/content/detail/R-HSA-70982" } ], "route": "tca", "compartment": "mitochondrial matrix", "representation": "single_event", "visual_style": "solid", "direction_note": "Stereochemistry is preserved in the product name.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-21433", "name": "Malate dehydrogenase 2", "equation": "(S)-malate + NAD(+) =\u003e oxaloacetate + NADH + H(+)", "source_id": "rhea", "source_record_ids": [ "RHEA:21433" ], "source_url": "https://www.rhea-db.org/rhea/21433", "rhea_parent_id": "RHEA:21432", "human_support": [ { "source": "Reactome", "id": "R-HSA-70979", "url": "https://reactome.org/content/detail/R-HSA-70979" } ], "route": "tca", "compartment": "mitochondrial matrix", "representation": "single_event", "visual_style": "solid", "direction_note": "Human Reactome display direction; no thermodynamic or flux claim follows from the arrow.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "reactome-212651", "name": "Glutamine import — BlackBoxEvent", "equation": "", "source_id": "reactome", "source_record_ids": [ "R-HSA-212651" ], "source_url": "https://reactome.org/content/detail/R-HSA-212651", "rhea_parent_id": "", "human_support": [], "route": "transport", "compartment": "cytosol to mitochondrial matrix", "representation": "transport_black_box", "visual_style": "dotted", "direction_note": "A curated BlackBoxEvent, not a chemical reaction or passive-diffusion claim.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": true, "transport_species": [ "L-glutamine (cytosol)", "L-glutamine (mitochondrial matrix)" ], "qualifying_language": "" }, { "id": "rhea-15890", "name": "Glutaminase", "equation": "L-glutamine + H2O =\u003e L-glutamate + NH4(+)", "source_id": "rhea", "source_record_ids": [ "RHEA:15890" ], "source_url": "https://www.rhea-db.org/rhea/15890", "rhea_parent_id": "RHEA:15889", "human_support": [ { "source": "Reactome", "id": "R-HSA-70609", "url": "https://reactome.org/content/detail/R-HSA-70609" }, { "source": "UniProtKB", "id": "O94925", "url": "https://www.uniprot.org/uniprotkb/O94925/entry" }, { "source": "UniProtKB", "id": "Q9UI32", "url": "https://www.uniprot.org/uniprotkb/Q9UI32/entry" } ], "route": "amino-entry", "compartment": "mitochondrial matrix", "representation": "single_event", "visual_style": "solid", "direction_note": "Matrix glutamine-to-glutamate connection after the separately represented import event.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-15134", "name": "Glutamate dehydrogenase — NAD-dependent", "equation": "L-glutamate + NAD(+) + H2O =\u003e 2-oxoglutarate + NH4(+) + NADH + H(+)", "source_id": "rhea", "source_record_ids": [ "RHEA:15134" ], "source_url": "https://www.rhea-db.org/rhea/15134", "rhea_parent_id": "RHEA:15133", "human_support": [ { "source": "Reactome", "id": "R-HSA-70600", "url": "https://reactome.org/content/detail/R-HSA-70600" }, { "source": "UniProtKB", "id": "P00367", "url": "https://www.uniprot.org/uniprotkb/P00367/entry" }, { "source": "UniProtKB", "id": "P49448", "url": "https://www.uniprot.org/uniprotkb/P49448/entry" } ], "route": "amino-entry", "compartment": "mitochondrial matrix", "representation": "cofactor_specific_alternative", "visual_style": "solid", "direction_note": "NAD-dependent equation remains distinct from the NADP-dependent alternative.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-11613", "name": "Glutamate dehydrogenase — NADP-dependent", "equation": "L-glutamate + NADP(+) + H2O =\u003e 2-oxoglutarate + NH4(+) + NADPH + H(+)", "source_id": "rhea", "source_record_ids": [ "RHEA:11613" ], "source_url": "https://www.rhea-db.org/rhea/11613", "rhea_parent_id": "RHEA:11612", "human_support": [ { "source": "Reactome", "id": "R-HSA-70600", "url": "https://reactome.org/content/detail/R-HSA-70600" }, { "source": "UniProtKB", "id": "P00367", "url": "https://www.uniprot.org/uniprotkb/P00367/entry" }, { "source": "UniProtKB", "id": "P49448", "url": "https://www.uniprot.org/uniprotkb/P49448/entry" } ], "route": "amino-entry", "compartment": "mitochondrial matrix", "representation": "cofactor_specific_alternative", "visual_style": "solid", "direction_note": "NADP-dependent equation remains distinct from the NAD-dependent alternative.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-21825", "name": "Aspartate connection through GOT2", "equation": "L-aspartate + 2-oxoglutarate =\u003e oxaloacetate + L-glutamate", "source_id": "rhea", "source_record_ids": [ "RHEA:21825" ], "source_url": "https://www.rhea-db.org/rhea/21825", "rhea_parent_id": "RHEA:21824", "human_support": [ { "source": "Reactome", "id": "R-HSA-70596", "url": "https://reactome.org/content/detail/R-HSA-70596" }, { "source": "UniProtKB", "id": "P00505", "url": "https://www.uniprot.org/uniprotkb/P00505/entry" } ], "route": "amino-entry", "compartment": "mitochondrial matrix", "representation": "tca_pool_interconversion", "visual_style": "solid", "direction_note": "A TCA-pool interconversion, not standalone evidence of net anaplerosis.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-23721", "name": "Propionyl-CoA carboxylase", "equation": "propanoyl-CoA + hydrogencarbonate + ATP =\u003e (S)-methylmalonyl-CoA + ADP + phosphate + H(+)", "source_id": "rhea", "source_record_ids": [ "RHEA:23721" ], "source_url": "https://www.rhea-db.org/rhea/23721", "rhea_parent_id": "RHEA:23720", "human_support": [ { "source": "Reactome", "id": "R-HSA-71031", "url": "https://reactome.org/content/detail/R-HSA-71031" }, { "source": "UniProtKB", "id": "P05165", "url": "https://www.uniprot.org/uniprotkb/P05165/entry" }, { "source": "UniProtKB", "id": "P05166", "url": "https://www.uniprot.org/uniprotkb/P05166/entry" } ], "route": "propionyl-entry", "compartment": "mitochondrial matrix", "representation": "single_event", "visual_style": "solid", "direction_note": "Branch begins at propanoyl-CoA; no common upstream origin is implied.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-20555", "name": "Methylmalonyl-CoA epimerase", "equation": "(S)-methylmalonyl-CoA =\u003e (R)-methylmalonyl-CoA", "source_id": "rhea", "source_record_ids": [ "RHEA:20555" ], "source_url": "https://www.rhea-db.org/rhea/20555", "rhea_parent_id": "RHEA:20553", "human_support": [ { "source": "Reactome", "id": "R-HSA-71020", "url": "https://reactome.org/content/detail/R-HSA-71020" }, { "source": "UniProtKB", "id": "Q96PE7", "url": "https://www.uniprot.org/uniprotkb/Q96PE7/entry" } ], "route": "propionyl-entry", "compartment": "mitochondrial matrix", "representation": "single_event", "visual_style": "solid", "direction_note": "Stereochemical identities are preserved.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-22889", "name": "Methylmalonyl-CoA mutase", "equation": "(R)-methylmalonyl-CoA =\u003e succinyl-CoA", "source_id": "rhea", "source_record_ids": [ "RHEA:22889" ], "source_url": "https://www.rhea-db.org/rhea/22889", "rhea_parent_id": "RHEA:22888", "human_support": [ { "source": "Reactome", "id": "R-HSA-71010", "url": "https://reactome.org/content/detail/R-HSA-71010" }, { "source": "UniProtKB", "id": "P22033", "url": "https://www.uniprot.org/uniprotkb/P22033/entry" } ], "route": "propionyl-entry", "compartment": "mitochondrial matrix", "representation": "single_event", "visual_style": "solid", "direction_note": "Matrix connection to succinyl-CoA; no downstream flux is implied.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-10389", "name": "Mitochondrial phosphoenolpyruvate carboxykinase", "equation": "oxaloacetate + GTP =\u003e phosphoenolpyruvate + GDP + CO2", "source_id": "rhea", "source_record_ids": [ "RHEA:10389" ], "source_url": "https://www.rhea-db.org/rhea/10389", "rhea_parent_id": "RHEA:10388", "human_support": [ { "source": "Reactome", "id": "R-HSA-372819", "url": "https://reactome.org/content/detail/R-HSA-372819" }, { "source": "UniProtKB", "id": "Q16822", "url": "https://www.uniprot.org/uniprotkb/Q16822/entry" } ], "route": "cataplerosis", "compartment": "mitochondrial matrix", "representation": "single_event", "visual_style": "solid", "direction_note": "PCK2 matrix reaction. PEP transport is not inferred by this chemical edge.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "rhea-18254", "name": "Mitochondrial NADP-dependent malic enzyme", "equation": "(S)-malate + NADP(+) =\u003e pyruvate + CO2 + NADPH", "source_id": "rhea", "source_record_ids": [ "RHEA:18254" ], "source_url": "https://www.rhea-db.org/rhea/18254", "rhea_parent_id": "RHEA:18253", "human_support": [ { "source": "Reactome", "id": "R-HSA-9012349", "url": "https://reactome.org/content/detail/R-HSA-9012349" }, { "source": "UniProtKB", "id": "Q16798", "url": "https://www.uniprot.org/uniprotkb/Q16798/entry" } ], "route": "cataplerosis", "compartment": "mitochondrial matrix", "representation": "single_event", "visual_style": "solid", "direction_note": "ME3 matrix reaction. No cross-membrane movement or net tissue flux is inferred.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" }, { "id": "reactome-75849", "name": "Citrate / malate exchange", "equation": "", "source_id": "reactome", "source_record_ids": [ "R-HSA-75849" ], "source_url": "https://reactome.org/content/detail/R-HSA-75849", "rhea_parent_id": "", "human_support": [], "route": "transport", "compartment": "mitochondrial inner membrane", "representation": "transport_exchange", "visual_style": "dotted", "direction_note": "SLC25A1-mediated exchange, not passive citrate diffusion or a one-way chemical reaction.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": true, "transport_species": [ "citrate (mitochondrial matrix)", "citrate (cytosol)", "(S)-malate (cytosol)", "(S)-malate (mitochondrial matrix)" ], "qualifying_language": "exchange" }, { "id": "rhea-21162", "name": "ATP-citrate lyase", "equation": "citrate + ATP + CoA =\u003e oxaloacetate + acetyl-CoA + ADP + phosphate", "source_id": "rhea", "source_record_ids": [ "RHEA:21162" ], "source_url": "https://www.rhea-db.org/rhea/21162", "rhea_parent_id": "", "human_support": [ { "source": "Reactome", "id": "R-HSA-75848", "url": "https://reactome.org/content/detail/R-HSA-75848" }, { "source": "UniProtKB", "id": "P53396", "url": "https://www.uniprot.org/uniprotkb/P53396/entry" } ], "route": "cataplerosis", "compartment": "cytosol", "representation": "single_event", "visual_style": "solid", "direction_note": "Cytosolic cleavage after the separately represented citrate/malate exchange; no downstream lipid use is asserted.", "confidence_status": "audited-source-linked", "crosses_compartment_boundary": false, "transport_species": [], "qualifying_language": "" } ], "events": [ { "id": "R-HSA-109278", "label": "NT5E:Zn2+ hydrolyses AMP,dAMP,GMP, IMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:29376", "RHEA:29372", "RHEA:27715", "RHEA:27719" ], "source_url": "https://reactome.org/content/detail/R-HSA-109278", "summary": "NT5E:Zn2+ hydrolyses AMP,dAMP,GMP, IMP is a Reactome release 97 curated reaction event. The source record connects AMP, dAMP, GMP, IMP, H2O to Ade-Rib, dA, Gua-Rib, Ino, Pi in extracellular region · plasma membrane and cross-references RHEA:29376, RHEA:29372, RHEA:27715, RHEA:27719.", "equation": "AMP, dAMP, GMP, IMP + H2O → Ade-Rib, dA, Gua-Rib, Ino + Pi", "inputs": [ { "node_id": "R-ALL-500101", "reactome_id": "R-ALL-500101", "label": "AMP, dAMP, GMP, IMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500104", "reactome_id": "R-ALL-500104", "label": "Ade-Rib, dA, Gua-Rib, Ino", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5E:Zn2+ dimer [plasma membrane]", "activity_id": "109273", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:3036115", "title": "Purification of 5'-nucleotidase from human placenta after release from plasma membranes by phosphatidylinositol-specific phospholipase C", "journal": "Biochem Biophys Res Commun", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3036115/" }, { "id": "PMID:2129526", "title": "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2129526/" }, { "id": "PMID:1637327", "title": "5'-nucleotidase: molecular structure and functional aspects", "journal": "Biochem J", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1637327/" } ], "x": 2938.666666666667, "y": 3053.1785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109291", "label": "CMP or TMP or UMP + H2O =\u003e cytidine, thymidine, or uridine + orthophosphate [NT5E]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:29368", "RHEA:11081", "RHEA:29360" ], "source_url": "https://reactome.org/content/detail/R-HSA-109291", "summary": "CMP or TMP or UMP + H2O =\u003e cytidine, thymidine, or uridine + orthophosphate [NT5E] is a Reactome release 97 curated reaction event. The source record connects CMP, TMP, UMP, H2O to Cyt-Rib, Thy-dRib, Ura-Rib, Pi in extracellular region · plasma membrane and cross-references RHEA:29368, RHEA:11081, RHEA:29360.", "equation": "CMP, TMP, UMP + H2O → Cyt-Rib, Thy-dRib, Ura-Rib + Pi", "inputs": [ { "node_id": "R-ALL-500316", "reactome_id": "R-ALL-500316", "label": "CMP, TMP, UMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500318", "reactome_id": "R-ALL-500318", "label": "Cyt-Rib, Thy-dRib, Ura-Rib", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5E:Zn2+ dimer [plasma membrane]", "activity_id": "109273", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:2129526", "title": "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2129526/" }, { "id": "PMID:2826576", "title": "Distribution of ecto-5'-nucleotidase on subsets of human T and B lymphocytes as detected by indirect immunofluorescence using goat antibodies", "journal": "J. Immunol.", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2826576/" }, { "id": "PMID:16377327", "title": "The importance of having a mentor, and how to find one", "journal": "Gastrointest. Endosc.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16377327/" } ], "x": 2938.666666666667, "y": 3074.535714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109338", "label": "Isomerization of cis,cis-3,6-Dodecadienoyl-CoA to form trans,cis-Lauro-2,6-dienoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-109338", "summary": "Isomerization of cis,cis-3,6-Dodecadienoyl-CoA to form trans,cis-Lauro-2,6-dienoyl-CoA is a Reactome release 97 curated reaction event. The source record connects cis,cis-3,6-Dodecadienoyl-CoA to trans,cis-Lauro-2,6-dienoyl-CoA in mitochondrial matrix.", "equation": "cis,cis-3,6-Dodecadienoyl-CoA → trans,cis-Lauro-2,6-dienoyl-CoA", "inputs": [ { "node_id": "R-ALL-77358", "reactome_id": "R-ALL-77358", "label": "cis,cis-3,6-Dodecadienoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78550", "formula": "C33H50N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-77360", "reactome_id": "R-ALL-77360", "label": "trans,cis-Lauro-2,6-dienoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28387", "formula": "C33H54N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "delta(3)-delta(2)-enoyl-CoA isomerase activity of DCI dimer [mitochondrial matrix]", "activity_id": "109984", "ec_numbers": [ "5.3.3.8" ], "accessions": [] } ], "citations": [], "x": 868.6666666666667, "y": 1727.6315789473683, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109339", "label": "Linoleoyl-CoA is converted to cis,cis-3,6- dodecadienoyl-CoA by three cycles of beta-oxidation", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-109339", "summary": "Linoleoyl-CoA is converted to cis,cis-3,6- dodecadienoyl-CoA by three cycles of beta-oxidation is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects LIN-CoA, FAD, H2O to cis,cis-3,6-Dodecadienoyl-CoA, FADH2, NADH in mitochondrial matrix.", "equation": "LIN-CoA + 3 FAD + 3 H2O + 3 NAD+ + 3 CoA-SH → cis,cis-3,6-Dodecadienoyl-CoA + 3 FADH2 + 3 NADH + 3 H+ + 3 Ac-CoA", "inputs": [ { "node_id": "R-ALL-77359", "reactome_id": "R-ALL-77359", "label": "LIN-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57383", "formula": "C39H62N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77358", "reactome_id": "R-ALL-77358", "label": "cis,cis-3,6-Dodecadienoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78550", "formula": "C33H50N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [], "x": 868.6666666666667, "y": 1724.6140350877192, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-109341", "label": "dehydrogenation of 4-cis-decenoyl-CoA to form 2-trans-4-cis-decadienoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-109341", "summary": "dehydrogenation of 4-cis-decenoyl-CoA to form 2-trans-4-cis-decadienoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 4-cis-decenoyl-CoA, FAD to 2-trans-4-cis-decadienoyl-CoA, FADH2 in mitochondrial matrix.", "equation": "4-cis-decenoyl-CoA + FAD → 2-trans-4-cis-decadienoyl-CoA + FADH2", "inputs": [ { "node_id": "R-ALL-109986", "reactome_id": "R-ALL-109986", "label": "4-cis-decenoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29140", "formula": "C31H52N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-109990", "reactome_id": "R-ALL-109990", "label": "2-trans-4-cis-decadienoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:137593", "formula": "C31H46N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADM tetramer [mitochondrial matrix]", "activity_id": "77336", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [], "x": 311.33333333333337, "y": 1712.5438596491229, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109342", "label": "Trans,cis-lauro-2,6-dienoyl-CoA is converted to cis,cis-3,6- 4-cis-decenoyl-CoA by one cycle of beta-oxidation", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-109342", "summary": "Trans,cis-lauro-2,6-dienoyl-CoA is converted to cis,cis-3,6- 4-cis-decenoyl-CoA by one cycle of beta-oxidation is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects trans,cis-Lauro-2,6-dienoyl-CoA, FAD, H2O to 4-cis-decenoyl-CoA, FADH2, NADH in mitochondrial matrix.", "equation": "trans,cis-Lauro-2,6-dienoyl-CoA + FAD + H2O + NAD+ + CoA-SH → 4-cis-decenoyl-CoA + FADH2 + NADH + Ac-CoA + H+", "inputs": [ { "node_id": "R-ALL-77360", "reactome_id": "R-ALL-77360", "label": "trans,cis-Lauro-2,6-dienoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28387", "formula": "C33H54N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-109986", "reactome_id": "R-ALL-109986", "label": "4-cis-decenoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29140", "formula": "C31H52N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [], "x": 311.33333333333337, "y": 1727.6315789473683, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-109343", "label": "Reduction of 2-trans-4-cis-decadienoyl-CoA to form 3-trans-decenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-109343", "summary": "Reduction of 2-trans-4-cis-decadienoyl-CoA to form 3-trans-decenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 2-trans-4-cis-decadienoyl-CoA, NADH, H+ to 3-trans-decenoyl-CoA, NAD+ in mitochondrial matrix.", "equation": "2-trans-4-cis-decadienoyl-CoA + NADH + H+ → 3-trans-decenoyl-CoA + NAD+", "inputs": [ { "node_id": "R-ALL-109990", "reactome_id": "R-ALL-109990", "label": "2-trans-4-cis-decadienoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:137593", "formula": "C31H46N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-109994", "reactome_id": "R-ALL-109994", "label": "3-trans-decenoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84793", "formula": "C31H48N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H of DECR1 tetramer [mitochondrial matrix]", "activity_id": "109993", "ec_numbers": [ "1.6" ], "accessions": [] } ], "citations": [], "x": 590, "y": 1706.5087719298244, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109380", "label": "(d)CMP, TMP, or (d)UMP + H2O =\u003e (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C1A)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11081", "RHEA:29368", "RHEA:29364", "RHEA:29360", "RHEA:29356" ], "source_url": "https://reactome.org/content/detail/R-HSA-109380", "summary": "(d)CMP, TMP, or (d)UMP + H2O =\u003e (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C1A) is a Reactome release 97 curated reaction event. The source record connects (d)CMP, TMP, (d)UMP, H2O to (d)C, T, (d)U, Pi in cytosol and cross-references RHEA:11081, RHEA:29368, RHEA:29364, RHEA:29360, RHEA:29356.", "equation": "(d)CMP, TMP, (d)UMP + H2O → (d)C, T, (d)U + Pi", "inputs": [ { "node_id": "R-ALL-500347", "reactome_id": "R-ALL-500347", "label": "(d)CMP, TMP, (d)UMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500346", "reactome_id": "R-ALL-500346", "label": "(d)C, T, (d)U", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5C1A tetramer [cytosol]", "activity_id": "109365", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:12947102", "title": "Mammalian 5'-nucleotidases", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12947102/" }, { "id": "PMID:11133996", "title": "Human cytosolic 5'-nucleotidase I. Characterization and role in nucleoside analog resistance", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11133996/" }, { "id": "PMID:10364222", "title": "The mechanism of adenosine formation in cells. Cloning of cytosolic 5'-nucleotidase-I", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10364222/" }, { "id": "PMID:2344353", "title": "Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart", "journal": "Biochem J", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2344353/" } ], "x": 3321.3333333333335, "y": 3356.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109387", "label": "(2'-deoxy)purine nucleoside 5'-monophosphate + H2O =\u003e (2'-deoxy)purine nucleoside + orthophosphate [NT5C1A]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:29376", "RHEA:29372", "RHEA:27715", "RHEA:29380", "RHEA:27719", "RHEA:29384" ], "source_url": "https://reactome.org/content/detail/R-HSA-109387", "summary": "(2'-deoxy)purine nucleoside 5'-monophosphate + H2O =\u003e (2'-deoxy)purine nucleoside + orthophosphate [NT5C1A] is a Reactome release 97 curated reaction event. The source record connects (d)AMP, (d)GMP, (d)IMP, H2O to (d)A, (d)G, (d)I, Pi in cytosol and cross-references RHEA:29376, RHEA:29372, RHEA:27715, RHEA:29380, RHEA:27719, RHEA:29384.", "equation": "(d)AMP, (d)GMP, (d)IMP + H2O → (d)A, (d)G, (d)I + Pi", "inputs": [ { "node_id": "R-ALL-500125", "reactome_id": "R-ALL-500125", "label": "(d)AMP, (d)GMP, (d)IMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500124", "reactome_id": "R-ALL-500124", "label": "(d)A, (d)G, (d)I", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5C1A tetramer [cytosol]", "activity_id": "109365", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:12947102", "title": "Mammalian 5'-nucleotidases", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12947102/" }, { "id": "PMID:11133996", "title": "Human cytosolic 5'-nucleotidase I. Characterization and role in nucleoside analog resistance", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11133996/" }, { "id": "PMID:10364222", "title": "The mechanism of adenosine formation in cells. Cloning of cytosolic 5'-nucleotidase-I", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10364222/" }, { "id": "PMID:2344353", "title": "Partial purification and properties of an AMP-specific soluble 5'-nucleotidase from pigeon heart", "journal": "Biochem J", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2344353/" } ], "x": 2381.3333333333335, "y": 3356.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109415", "label": "AMP + H2O =\u003e adenosine + orthophosphate [NT5C1B]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:29376" ], "source_url": "https://reactome.org/content/detail/R-HSA-109415", "summary": "AMP + H2O =\u003e adenosine + orthophosphate [NT5C1B] is a Reactome release 97 curated reaction event. The source record connects AMP, H2O to Ade-Rib, Pi in cytosol and cross-references RHEA:29376.", "equation": "AMP + H2O → Ade-Rib + Pi", "inputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-83909", "reactome_id": "R-ALL-83909", "label": "Ade-Rib", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16335", "formula": "C10H13N5O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5C1B [cytosol]", "activity_id": "109412", "ec_numbers": [ "3.1.3.5" ], "accessions": [ "Q96P26" ] } ], "citations": [ { "id": "PMID:11690631", "title": "Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein", "journal": "Biochim Biophys Acta", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11690631/" }, { "id": "PMID:12750059", "title": "Metabolic and functional consequences of cytosolic 5'-nucleotidase-IA overexpression in neonatal rat cardiomyocytes", "journal": "Am J Physiol Heart Circ Physiol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12750059/" } ], "x": 2660, "y": 3159.964285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109449", "label": "(d)CMP, TMP, or (d)UMP + H2O =\u003e (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C3)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11081", "RHEA:29368", "RHEA:29364", "RHEA:29360", "RHEA:29356" ], "source_url": "https://reactome.org/content/detail/R-HSA-109449", "summary": "(d)CMP, TMP, or (d)UMP + H2O =\u003e (deoxy)cytidine, thymidine, or (deoxy)uridine + orthophosphate (NT5C3) is a Reactome release 97 curated reaction event. The source record connects (d)CMP, TMP, (d)UMP, H2O to (d)C, T, (d)U, Pi in cytosol and cross-references RHEA:11081, RHEA:29368, RHEA:29364, RHEA:29360, RHEA:29356.", "equation": "(d)CMP, TMP, (d)UMP + H2O → (d)C, T, (d)U + Pi", "inputs": [ { "node_id": "R-ALL-500347", "reactome_id": "R-ALL-500347", "label": "(d)CMP, TMP, (d)UMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500346", "reactome_id": "R-ALL-500346", "label": "(d)C, T, (d)U", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5C3 holoenzyme [cytosol]", "activity_id": "109441", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:11795870", "title": "Human erythrocyte pyrimidine 5'-nucleotidase, PN-I", "journal": "Arch Biochem Biophys", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11795870/" }, { "id": "PMID:9428647", "title": "Pyrimidine nucleotidases from human erythrocyte possess phosphotransferase activities specific for pyrimidine nucleotides", "journal": "FEBS Lett", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9428647/" }, { "id": "PMID:11369620", "title": "Genetic basis of hemolytic anemia caused by pyrimidine 5' nucleotidase deficiency", "journal": "Blood", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11369620/" }, { "id": "PMID:12580951", "title": "Pyrimidine 5' nucleotidase deficiency", "journal": "Br J Haematol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12580951/" } ], "x": 2381.3333333333335, "y": 3053.1785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109470", "label": "(d)GMP or (d)IMP + H2O =\u003e (d)G or (d)I + orthophosphate (NT5C)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27715", "RHEA:29380", "RHEA:27719", "RHEA:29384" ], "source_url": "https://reactome.org/content/detail/R-HSA-109470", "summary": "(d)GMP or (d)IMP + H2O =\u003e (d)G or (d)I + orthophosphate (NT5C) is a Reactome release 97 curated reaction event. The source record connects (d)GMP, (d)IMP, H2O to Gu-Rib, dG, Ino, dI, Pi in cytosol and cross-references RHEA:27715, RHEA:29380, RHEA:27719, RHEA:29384.", "equation": "(d)GMP, (d)IMP + H2O → Gu-Rib, dG, Ino, dI + Pi", "inputs": [ { "node_id": "R-ALL-500120", "reactome_id": "R-ALL-500120", "label": "(d)GMP, (d)IMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500118", "reactome_id": "R-ALL-500118", "label": "Gu-Rib, dG, Ino, dI", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5C dimer [cytosol]", "activity_id": "109469", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:2157703", "title": "Cytoplasmic 5'(3')-nucleotidase from human placenta", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2157703/" }, { "id": "PMID:11083867", "title": "Cytosolic high Km 5'-nucleotidase and 5'(3')-deoxyribonucleotidase in substrate cycles involved in nucleotide metabolism", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11083867/" }, { "id": "PMID:10681516", "title": "Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681516/" } ], "x": 2381.3333333333335, "y": 3060.2976190476193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109480", "label": "TMP, uridine 2', 3', or 5' monophosphates, or deoxyuridine 3' or 5' monophosphates + H2O =\u003e thymidine or (deoxy)uridine + orthophosphate [NT5C]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11081", "RHEA:37356", "RHEA:27891", "RHEA:29360", "RHEA:29356" ], "source_url": "https://reactome.org/content/detail/R-HSA-109480", "summary": "TMP, uridine 2', 3', or 5' monophosphates, or deoxyuridine 3' or 5' monophosphates + H2O =\u003e thymidine or (deoxy)uridine + orthophosphate [NT5C] is a Reactome release 97 curated reaction event. The source record connects TMP, U2MP, U3MP, UMP, dU3MP, dUMP, H2O to Thy-Rib, (d)U, Pi in cytosol and cross-references RHEA:11081, RHEA:37356, RHEA:27891, RHEA:29360, RHEA:29356.", "equation": "TMP, U2MP, U3MP, UMP, dU3MP, dUMP + H2O → Thy-Rib, (d)U + Pi", "inputs": [ { "node_id": "R-ALL-500414", "reactome_id": "R-ALL-500414", "label": "TMP, U2MP, U3MP, UMP, dU3MP, dUMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500415", "reactome_id": "R-ALL-500415", "label": "Thy-Rib, (d)U", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5C dimer [cytosol]", "activity_id": "109469", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:11083867", "title": "Cytosolic high Km 5'-nucleotidase and 5'(3')-deoxyribonucleotidase in substrate cycles involved in nucleotide metabolism", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11083867/" }, { "id": "PMID:2157703", "title": "Cytoplasmic 5'(3')-nucleotidase from human placenta", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2157703/" }, { "id": "PMID:10681516", "title": "Mammalian 5'(3')-deoxyribonucleotidase, cDNA cloning, and overexpression of the enzyme in Escherichia coli and mammalian cells", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681516/" } ], "x": 2660, "y": 3060.2976190476193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109514", "label": "TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O =\u003e thymidine, deoxyuridine, or uridine + orthophosphate [NT5M]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:11081", "RHEA:29360", "RHEA:29356", "RHEA:37356", "RHEA:27891" ], "source_url": "https://reactome.org/content/detail/R-HSA-109514", "summary": "TMP, (d)UMP, uridine 2' monophosphate, or uridine 3'-monophosphate + H2O =\u003e thymidine, deoxyuridine, or uridine + orthophosphate [NT5M] is a Reactome release 97 curated reaction event. The source record connects TMP, UMP, dU5MP, U2MP, U3MP, H2O to Thy-dRib, Ura-Rib, dU, Pi in mitochondrial matrix and cross-references RHEA:11081, RHEA:29360, RHEA:29356, RHEA:37356, RHEA:27891.", "equation": "TMP, UMP, dU5MP, U2MP, U3MP + H2O → Thy-dRib, Ura-Rib, dU + Pi", "inputs": [ { "node_id": "R-ALL-500420", "reactome_id": "R-ALL-500420", "label": "TMP, UMP, dU5MP, U2MP, U3MP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500421", "reactome_id": "R-ALL-500421", "label": "Thy-dRib, Ura-Rib, dU", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5M dimer [mitochondrial matrix]", "activity_id": "109498", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:10899995", "title": "A deoxynucleotidase in mitochondria: involvement in regulation of dNTP pools and possible link to genetic disease", "journal": "Proc Natl Acad Sci USA", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10899995/" }, { "id": "PMID:12124385", "title": "Human mitochondrial 5'-deoxynucleotidase. Overproduction in cultured cells and functional aspects", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12124385/" }, { "id": "PMID:12352955", "title": "Crystal structure of a human mitochondrial deoxyribonuclease", "journal": "Nt Struct Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12352955/" } ], "x": 2938.666666666667, "y": 3060.2976190476193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109624", "label": "(2'-deoxy)adenosine + ATP =\u003e (d)AMP + ADP (ADK)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20825" ], "source_url": "https://reactome.org/content/detail/R-HSA-109624", "summary": "(2'-deoxy)adenosine + ATP =\u003e (d)AMP + ADP (ADK) is a Reactome release 97 curated reaction event. The source record connects Ade-Rib, dA, ATP to (d)AMP, ADP, H+ in cytosol and cross-references RHEA:20825.", "equation": "Ade-Rib, dA + ATP → (d)AMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-500173", "reactome_id": "R-ALL-500173", "label": "Ade-Rib, dA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500088", "reactome_id": "R-ALL-500088", "label": "(d)AMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "adenosine kinase activity of ADK [cytosol]", "activity_id": "76547", "ec_numbers": [ "2.7.1.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:2999129", "title": "Regulation of deoxyadenosine and nucleoside analog phosphorylation by human placental adenosine kinase", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2999129/" }, { "id": "PMID:6281270", "title": "Effects of mutational loss of adenosine kinase and deoxycytidine kinase on deoxyATP accumulation and deoxyadenosine toxicity in cultured CEM human T-lymphoblastoid cells", "journal": "J Biol Chem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6281270/" }, { "id": "PMID:9843365", "title": "Structure of human adenosine kinase at 1.5 A resolution.", "journal": "Biochemistry", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9843365/" }, { "id": "PMID:227870", "title": "Purification and properties of human placental adenosine kinase", "journal": "J Biol Chem", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/227870/" } ], "x": 3600, "y": 2941.8214285714284, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109671", "label": "deoxyadenosine or deoxyguanosine + ATP =\u003e dAMP or dGMP + ADP (DCK)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23453", "RHEA:19202" ], "source_url": "https://reactome.org/content/detail/R-HSA-109671", "summary": "deoxyadenosine or deoxyguanosine + ATP =\u003e dAMP or dGMP + ADP (DCK) is a Reactome release 97 curated reaction event. The source record connects dA, dG, ATP to dAMP, dGMP, ADP, H+ in cytosol and cross-references RHEA:23453, RHEA:19202.", "equation": "dA, dG + ATP → dAMP, dGMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-500201", "reactome_id": "R-ALL-500201", "label": "dA, dG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500199", "reactome_id": "R-ALL-500199", "label": "dAMP, dGMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside kinase activity of DCK dimer [cytosol]", "activity_id": "73517", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:2844225", "title": "Deoxycytidine kinase from human leukemic spleen: preparation and characterization of the homogeneous enzyme", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2844225/" }, { "id": "PMID:2539852", "title": "Human T-lymphoblast deoxycytidine kinase: purification and properties", "journal": "Biochemistry", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2539852/" }, { "id": "PMID:9804782", "title": "The intracellular localization of deoxycytidine kinase", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9804782/" } ], "x": 3600, "y": 2877.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109759", "label": "deoxycytidine, thymidine, or deoxyuridine + ATP =\u003e dCMP, TMP, or dUMP + ADP [TK2]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:46041", "RHEA:19130", "RHEA:28207" ], "source_url": "https://reactome.org/content/detail/R-HSA-109759", "summary": "deoxycytidine, thymidine, or deoxyuridine + ATP =\u003e dCMP, TMP, or dUMP + ADP [TK2] is a Reactome release 97 curated reaction event. The source record connects dC, Thy-dRib, dU, ATP to dCMP, TMP, dUMP, ADP, H+ in mitochondrial matrix and cross-references RHEA:46041, RHEA:19130, RHEA:28207.", "equation": "dC, Thy-dRib, dU + ATP → dCMP, TMP, dUMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-500822", "reactome_id": "R-ALL-500822", "label": "dC, Thy-dRib, dU", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500823", "reactome_id": "R-ALL-500823", "label": "dCMP, TMP, dUMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside kinase activity of TK2 [mitochondrial matrix]", "activity_id": "109753", "ec_numbers": null, "accessions": [ "O00142" ] } ], "citations": [ { "id": "PMID:1597187", "title": "Mammalian thymidine kinase 2. Direct photoaffinity labeling with [32P]dTTP of the enzyme from spleen, liver, heart and brain", "journal": "Eur J Biochem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1597187/" }, { "id": "PMID:1063125", "title": "Human deoxythymidine kinase. I. Purification and general properties of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia", "journal": "J Biol Chem", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/1063125/" }, { "id": "PMID:2026611", "title": "Diverging substrate specificity of pure human thymidine kinases 1 and 2 against antiviral dideoxynucleosides", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2026611/" }, { "id": "PMID:9989599", "title": "Human thymidine kinase 2: molecular cloning and characterisation of the enzyme activity with antiviral and cytostatic nucleoside substrates", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9989599/" } ], "x": 3321.3333333333335, "y": 2835.035714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109903", "label": "cytidine or uridine + ATP =\u003e CMP or UMP + ADP [UCK2]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24675", "RHEA:16826" ], "source_url": "https://reactome.org/content/detail/R-HSA-109903", "summary": "cytidine or uridine + ATP =\u003e CMP or UMP + ADP [UCK2] is a Reactome release 97 curated reaction event. The source record connects Cyt-Rib, Ura-Rib, ATP to CMP, UMP, ADP, H+ in cytosol and cross-references RHEA:24675, RHEA:16826.", "equation": "Cyt-Rib, Ura-Rib + ATP → CMP, UMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-500759", "reactome_id": "R-ALL-500759", "label": "Cyt-Rib, Ura-Rib", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500761", "reactome_id": "R-ALL-500761", "label": "CMP, UMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside kinase activity of UCK2 tetramer [cytosol]", "activity_id": "109900", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:195585", "title": "Uridine kinase activities and pyrimidine nucleoside phosphorylation in fluoropyrimidine-sensitive and -resistant cell lines of the Novikoff hepatoma", "journal": "Biochem J", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/195585/" }, { "id": "PMID:15130468", "title": "Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase", "journal": "Structure", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15130468/" }, { "id": "PMID:11306702", "title": "Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases", "journal": "Mol Pharmacol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11306702/" }, { "id": "PMID:14609716", "title": "Substrate specificity and phosphorylation of antiviral and anticancer nucleoside analogues by human deoxyribonucleoside kinases and ribonucleoside kinases", "journal": "Pharmacol Ther", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14609716/" } ], "x": 3878.666666666667, "y": 2877.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-109998", "label": "Isomerization of 3-trans-decenoyl-CoA to form trans-dec-2-enoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:45753" ], "source_url": "https://reactome.org/content/detail/R-HSA-109998", "summary": "Isomerization of 3-trans-decenoyl-CoA to form trans-dec-2-enoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-trans-decenoyl-CoA to tdec2-CoA in mitochondrial matrix and cross-references RHEA:45753.", "equation": "3-trans-decenoyl-CoA → tdec2-CoA", "inputs": [ { "node_id": "R-ALL-109994", "reactome_id": "R-ALL-109994", "label": "3-trans-decenoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:84793", "formula": "C31H48N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-77343", "reactome_id": "R-ALL-77343", "label": "tdec2-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61406", "formula": "C31H48N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "intramolecular oxidoreductase activity of DCI dimer [mitochondrial matrix]", "activity_id": "109997", "ec_numbers": [ "5.3" ], "accessions": [] } ], "citations": [], "x": 311.33333333333337, "y": 1625.0350877192982, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-110133", "label": "(d)GDP + ADP (d)GMP + ATP (GUK1)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12699", "RHEA:20782" ], "source_url": "https://reactome.org/content/detail/R-HSA-110133", "summary": "(d)GDP + ADP (d)GMP + ATP (GUK1) is a Reactome release 97 curated reaction event. The source record connects (d)GDP, ADP to (d)GMP, ATP in cytosol and cross-references RHEA:12699, RHEA:20782.", "equation": "(d)GDP + ADP → (d)GMP + ATP", "inputs": [ { "node_id": "R-ALL-500077", "reactome_id": "R-ALL-500077", "label": "(d)GDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500071", "reactome_id": "R-ALL-500071", "label": "(d)GMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "GMP kinase activity of GUK1 [cytosol]", "activity_id": "74189", "ec_numbers": [ "2.7.4.8" ], "accessions": [ "Q16774" ] } ], "citations": [ { "id": "PMID:211390", "title": "Guanylate kinases from human erythrocytes, hog brain, and rat liver", "journal": "Methods Enzymol", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/211390/" }, { "id": "PMID:8663313", "title": "Cloning, characterization, and modeling of mouse and human guanylate kinases", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8663313/" }, { "id": "PMID:177353", "title": "Studies on the properties and tissue distribution of the isozymes of guanylate kinase in man", "journal": "Human Hered", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/177353/" }, { "id": "PMID:6248551", "title": "Phosphorylation of acyclovir (acycloguanosine) monophosphate by GMP kinase", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6248551/" } ], "x": 3321.3333333333335, "y": 3086.1041666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-110137", "label": "(d)ADP or (d)CDP + ADP (d)AMP or (d)CMP + ATP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23102", "RHEA:12975", "RHEA:11602", "RHEA:25096" ], "source_url": "https://reactome.org/content/detail/R-HSA-110137", "summary": "(d)ADP or (d)CDP + ADP (d)AMP or (d)CMP + ATP is a Reactome release 97 curated reaction event. The source record connects (d)NDPs, ADP to (d)NMPs, ATP in cytosol and cross-references RHEA:23102, RHEA:12975, RHEA:11602, RHEA:25096.", "equation": "(d)NDPs + ADP → (d)NMPs + ATP", "inputs": [ { "node_id": "R-ALL-6788782", "reactome_id": "R-ALL-6788782", "label": "(d)NDPs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6788778", "reactome_id": "R-ALL-6788778", "label": "(d)NMPs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside kinase activity of AK5,7,8,9 [cytosol]", "activity_id": "110088", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10215863", "title": "Identification of a novel human adenylate kinase. cDNA cloning, expression analysis, chromosome localization and characterization of the recombinant protein", "journal": "Eur J Biochem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10215863/" } ], "x": 3321.3333333333335, "y": 3073.6458333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-110138", "label": "AK5,7,8,9 phosphorylates (d)NMPs to (d)NDPs", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23101", "RHEA:12974", "RHEA:11601", "RHEA:25095" ], "source_url": "https://reactome.org/content/detail/R-HSA-110138", "summary": "AK5,7,8,9 phosphorylates (d)NMPs to (d)NDPs is a Reactome release 97 curated reaction event. The source record connects (d)NMPs, ATP to (d)NDPs, ADP in cytosol and cross-references RHEA:23101, RHEA:12974, RHEA:11601, RHEA:25095.", "equation": "(d)NMPs + ATP → (d)NDPs + ADP", "inputs": [ { "node_id": "R-ALL-6788778", "reactome_id": "R-ALL-6788778", "label": "(d)NMPs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6788782", "reactome_id": "R-ALL-6788782", "label": "(d)NDPs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside kinase activity of AK5,7,8,9 [cytosol]", "activity_id": "110088", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10215863", "title": "Identification of a novel human adenylate kinase. cDNA cloning, expression analysis, chromosome localization and characterization of the recombinant protein", "journal": "Eur J Biochem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10215863/" }, { "id": "PMID:21080915", "title": "The characterization of human adenylate kinases 7 and 8 demonstrates differences in kinetic parameters and structural organization among the family of adenylate kinase isoenzymes", "journal": "Biochem. J.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21080915/" }, { "id": "PMID:23416111", "title": "The human adenylate kinase 9 is a nucleoside mono- and diphosphate kinase", "journal": "Int. J. Biochem. Cell Biol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23416111/" } ], "x": 3600, "y": 3073.6458333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-110141", "label": "(d)ADP + ADP (d)AMP + ATP (AK1)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12975", "RHEA:23102" ], "source_url": "https://reactome.org/content/detail/R-HSA-110141", "summary": "(d)ADP + ADP (d)AMP + ATP (AK1) is a Reactome release 97 curated reaction event. The source record connects (d)ADP, ADP to (d)AMP, ATP in cytosol and cross-references RHEA:12975, RHEA:23102.", "equation": "(d)ADP + ADP → (d)AMP + ATP", "inputs": [ { "node_id": "R-ALL-500087", "reactome_id": "R-ALL-500087", "label": "(d)ADP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500088", "reactome_id": "R-ALL-500088", "label": "(d)AMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "AMP kinase activity of Adenylate Kinase [cytosol]", "activity_id": "73829", "ec_numbers": [ "2.7.4.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:211388", "title": "AMP (dAMP) kinase from human erythrocytes", "journal": "Methods Enzymol", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/211388/" }, { "id": "PMID:2542324", "title": "Human adenylate kinase deficiency associated with hemolytic anemia. A single base substitution affecting solubility and catalytic activity of the cytosolic adenylate kinase.", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2542324/" } ], "x": 3600, "y": 3086.1041666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-110144", "label": "ADP + ADP AMP + ATP [AK2]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005829", "GO:0005758" ], "rhea_ids": [ "RHEA:12975" ], "source_url": "https://reactome.org/content/detail/R-HSA-110144", "summary": "ADP + ADP AMP + ATP [AK2] is a Reactome release 97 curated reaction event. The source record connects ADP to AMP, ATP in cytosol · mitochondrial intermembrane space and cross-references RHEA:12975.", "equation": "2 ADP → AMP + ATP", "inputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "AMP kinase activity of AK2 [mitochondrial intermembrane space]", "activity_id": "110089", "ec_numbers": [ "2.7.4.3" ], "accessions": [ "P54819" ] } ], "citations": [ { "id": "PMID:5484814", "title": "Rat liver adenosine triphosphate:adenosine monophosphate phosphotransferase activity. II. Subcellular localization of adenylate kinase isozymes", "journal": "J Biol Chem", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/5484814/" }, { "id": "PMID:6182143", "title": "Adenosine triphosphate-adenosine-5'-monophosphate phosphotransferase from normal human liver mitochondria. Isolation, chemical properties, and immunochemical comparison with Duchenne muscular dystrophic serum aberrant adenylate kinase", "journal": "J Biol Chem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6182143/" } ], "x": 3600, "y": 3135.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-110145", "label": "AMP + ATP ADP + ADP [AK2]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005829", "GO:0005758" ], "rhea_ids": [ "RHEA:12974" ], "source_url": "https://reactome.org/content/detail/R-HSA-110145", "summary": "AMP + ATP ADP + ADP [AK2] is a Reactome release 97 curated reaction event. The source record connects AMP, ATP to ADP in cytosol · mitochondrial intermembrane space and cross-references RHEA:12974.", "equation": "AMP + ATP → 2 ADP", "inputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "AMP kinase activity of AK2 [mitochondrial intermembrane space]", "activity_id": "110089", "ec_numbers": [ "2.7.4.3" ], "accessions": [ "P54819" ] } ], "citations": [ { "id": "PMID:5484814", "title": "Rat liver adenosine triphosphate:adenosine monophosphate phosphotransferase activity. II. Subcellular localization of adenylate kinase isozymes", "journal": "J Biol Chem", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/5484814/" }, { "id": "PMID:6182143", "title": "Adenosine triphosphate-adenosine-5'-monophosphate phosphotransferase from normal human liver mitochondria. Isolation, chemical properties, and immunochemical comparison with Duchenne muscular dystrophic serum aberrant adenylate kinase", "journal": "J Biol Chem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6182143/" } ], "x": 3878.666666666667, "y": 3135.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111215", "label": "PRPS1 dimer pyrophosphorylates R5P using dATP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-111215", "summary": "PRPS1 dimer pyrophosphorylates R5P using dATP is a Reactome release 97 curated reaction event. The source record connects R5P, dATP to PRPP, dAMP, H+ in cytosol.", "equation": "R5P + dATP → PRPP + dAMP + H+", "inputs": [ { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-110644", "reactome_id": "R-ALL-110644", "label": "dATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61404", "formula": "C10H12N5O12P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109386", "reactome_id": "R-ALL-109386", "label": "dAMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58245", "formula": "C10H12N5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribose phosphate diphosphokinase activity of PRPS1 dimer [cytosol]", "activity_id": "73486", "ec_numbers": [ "2.7.6.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:4328836", "title": "Human phosphoribosylpyrophosphate synthetase", "journal": "J Biol Chem", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/4328836/" }, { "id": "PMID:28031352", "title": "Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance", "journal": "Microbiol Mol Biol Rev", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28031352/" } ], "x": 590, "y": 1103.2142857142858, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111285", "label": "Formation of phosphoribosyl pyrophosphate amidotransferase tetramer", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-111285", "summary": "Formation of phosphoribosyl pyrophosphate amidotransferase tetramer is a Reactome release 97 curated reaction event. The source record connects PPAT dimer to PPAT tetramer in cytosol.", "equation": "2 PPAT dimer → PPAT tetramer", "inputs": [ { "node_id": "R-HSA-73790", "reactome_id": "R-HSA-73790", "label": "PPAT dimer", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-73789", "reactome_id": "R-HSA-73789", "label": "PPAT tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:4348202", "title": "Human glutamine phosphoribosylpyrophosphate amidotransferase. Kinetic and regulatory properties", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4348202/" }, { "id": "PMID:4726295", "title": "Human glutamine phosphoribosylpyrophosphate amidotransferase. Two molecular forms interconvertible by purine ribonucleotides and phosphoribosylpyrophosphate", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4726295/" } ], "x": 2938.666666666667, "y": 2924.46875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111289", "label": "Dissociation of phosphoribosyl pyrophosphate amidotransferase tetramer", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-111289", "summary": "Dissociation of phosphoribosyl pyrophosphate amidotransferase tetramer is a Reactome release 97 curated reaction event. The source record connects PPAT tetramer to PPAT dimer in cytosol.", "equation": "PPAT tetramer → 2 PPAT dimer", "inputs": [ { "node_id": "R-HSA-73789", "reactome_id": "R-HSA-73789", "label": "PPAT tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-73790", "reactome_id": "R-HSA-73790", "label": "PPAT dimer", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:4348202", "title": "Human glutamine phosphoribosylpyrophosphate amidotransferase. Kinetic and regulatory properties", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4348202/" }, { "id": "PMID:4726295", "title": "Human glutamine phosphoribosylpyrophosphate amidotransferase. Two molecular forms interconvertible by purine ribonucleotides and phosphoribosylpyrophosphate", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4726295/" } ], "x": 2381.3333333333335, "y": 2943.15625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111524", "label": "IMP + L-Aspartate + GTP =\u003e Adenylosuccinate + GDP + Pi [ADSS]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15754" ], "source_url": "https://reactome.org/content/detail/R-HSA-111524", "summary": "IMP + L-Aspartate + GTP =\u003e Adenylosuccinate + GDP + Pi [ADSS] is a Reactome release 97 curated reaction event. The source record connects GTP, IMP, L-Asp to GDP, Pi, ADS in cytosol and cross-references RHEA:15754.", "equation": "GTP + IMP + L-Asp → GDP + Pi + ADS + 2 H+", "inputs": [ { "node_id": "R-ALL-29438", "reactome_id": "R-ALL-29438", "label": "GTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37565", "formula": "C10H12N5O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29602", "reactome_id": "R-ALL-29602", "label": "IMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58053", "formula": "C10H11N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29448", "reactome_id": "R-ALL-29448", "label": "L-Asp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29420", "reactome_id": "R-ALL-29420", "label": "GDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58189", "formula": "C10H12N5O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-35141", "reactome_id": "R-ALL-35141", "label": "ADS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57567", "formula": "C14H14N5O11P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "adenylosuccinate synthase activity of ADSS, ADSSL1 dimers [cytosol]", "activity_id": "111492", "ec_numbers": [ "6.3.4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:1592113", "title": "Cloning and characterization of the cDNA encoding human adenylosuccinate synthetase", "journal": "FEBS Lett", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1592113/" }, { "id": "PMID:15786719", "title": "Molecular cloning and characterization of a novel muscle adenylosuccinate synthetase, AdSSL1, from human bone marrow stromal cells", "journal": "Mol Cell Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15786719/" } ], "x": 2381.3333333333335, "y": 2831.03125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111742", "label": "RNR (M1M2) reduces nucleotide diphosphates to deoxynucleotide diphosphates (glutaredoxin)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-111742", "summary": "RNR (M1M2) reduces nucleotide diphosphates to deoxynucleotide diphosphates (glutaredoxin) is a Reactome release 97 curated reaction event. The source record connects NDP(3-), GLRX to dNDP(3-), 2xHC-GLRX, H2O in cytosol.", "equation": "NDP(3-) + GLRX → dNDP(3-) + 2xHC-GLRX + H2O", "inputs": [ { "node_id": "R-ALL-8866401", "reactome_id": "R-ALL-8866401", "label": "NDP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-55729", "reactome_id": "R-HSA-55729", "label": "GLRX", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8866399", "reactome_id": "R-ALL-8866399", "label": "dNDP(3-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73316", "formula": "C5H8O9P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-111740", "reactome_id": "R-HSA-111740", "label": "2xHC-GLRX", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside-diphosphate reductase activity, glutaredoxin disulfide as acceptor of RNR (M1M2) [cytosol]", "activity_id": "73641", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11796141", "title": "Structure and function of the radical enzyme ribonucleotide reductase", "journal": "Prog Biophys Mol Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11796141/" }, { "id": "PMID:2668278", "title": "Thioredoxin and glutaredoxin systems", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2668278/" }, { "id": "PMID:16756507", "title": "Ribonucleotide reductases", "journal": "Annu Rev Biochem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16756507/" }, { "id": "PMID:18997010", "title": "Ribonucleotide reduction is a cytosolic process in mammalian cells independently of DNA damage", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18997010/" }, { "id": "PMID:10884394", "title": "Cross-talk between the allosteric effector-binding sites in mouse ribonucleotide reductase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10884394/" }, { "id": "PMID:9677297", "title": "The NMR solution structure of human glutaredoxin in the fully reduced form", "journal": "J Mol Biol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9677297/" } ], "x": 3878.666666666667, "y": 3173.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111746", "label": "glutaredoxin (oxidized) + glutathione (reduced) =\u003e glutaredoxin (reduced) + glutathione (oxidized)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21065" ], "source_url": "https://reactome.org/content/detail/R-HSA-111746", "summary": "glutaredoxin (oxidized) + glutathione (reduced) =\u003e glutaredoxin (reduced) + glutathione (oxidized) is a Reactome release 97 curated reaction event. The source record connects 2xHC-GLRX, GSH to GLRX, GSSG in cytosol and cross-references RHEA:21065.", "equation": "2xHC-GLRX + 2 GSH → GLRX + GSSG", "inputs": [ { "node_id": "R-HSA-111740", "reactome_id": "R-HSA-111740", "label": "2xHC-GLRX", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-55729", "reactome_id": "R-HSA-55729", "label": "GLRX", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione disulfide oxidoreductase activity of 2xHC-GLRX [cytosol]", "activity_id": "499937", "ec_numbers": null, "accessions": [ "P35754" ] } ], "citations": [ { "id": "PMID:7851394", "title": "Purification from placenta, amino acid sequence, structure comparisons and cDNA cloning of human glutaredoxin", "journal": "Eur J Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7851394/" } ], "x": 3321.3333333333335, "y": 3173.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111751", "label": "RNR (M1M2) reduces nucleotide diphosphates to deoxynucleotide diphosphates (thioredoxin)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23254" ], "source_url": "https://reactome.org/content/detail/R-HSA-111751", "summary": "RNR (M1M2) reduces nucleotide diphosphates to deoxynucleotide diphosphates (thioredoxin) is a Reactome release 97 curated reaction event. The source record connects NDP(3-), TXN to dNDP(3-), 2xHC-TXN, H2O in cytosol and cross-references RHEA:23254.", "equation": "NDP(3-) + TXN → dNDP(3-) + 2xHC-TXN + H2O", "inputs": [ { "node_id": "R-ALL-8866401", "reactome_id": "R-ALL-8866401", "label": "NDP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-66000", "reactome_id": "R-HSA-66000", "label": "TXN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8866399", "reactome_id": "R-ALL-8866399", "label": "dNDP(3-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73316", "formula": "C5H8O9P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-73668", "reactome_id": "R-HSA-73668", "label": "2xHC-TXN", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor of RNR (M1M2) [cytosol]", "activity_id": "8866404", "ec_numbers": [ "1.17.4.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11796141", "title": "Structure and function of the radical enzyme ribonucleotide reductase", "journal": "Prog Biophys Mol Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11796141/" }, { "id": "PMID:2668278", "title": "Thioredoxin and glutaredoxin systems", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2668278/" }, { "id": "PMID:16756507", "title": "Ribonucleotide reductases", "journal": "Annu Rev Biochem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16756507/" }, { "id": "PMID:18997010", "title": "Ribonucleotide reduction is a cytosolic process in mammalian cells independently of DNA damage", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18997010/" }, { "id": "PMID:10884394", "title": "Cross-talk between the allosteric effector-binding sites in mouse ribonucleotide reductase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10884394/" }, { "id": "PMID:9677297", "title": "The NMR solution structure of human glutaredoxin in the fully reduced form", "journal": "J Mol Biol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9677297/" } ], "x": 3321.3333333333335, "y": 3160.8541666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111804", "label": "RNR (M1M2B) reduces nucleotide diphosphates to deoxynucleotide diphosphates (thioredoxin)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23254" ], "source_url": "https://reactome.org/content/detail/R-HSA-111804", "summary": "RNR (M1M2B) reduces nucleotide diphosphates to deoxynucleotide diphosphates (thioredoxin) is a Reactome release 97 curated reaction event. The source record connects NDP(3-), TXN to dNDP(3-), 2xHC-TXN, H2O in cytosol and cross-references RHEA:23254.", "equation": "NDP(3-) + TXN → dNDP(3-) + 2xHC-TXN + H2O", "inputs": [ { "node_id": "R-ALL-8866401", "reactome_id": "R-ALL-8866401", "label": "NDP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-66000", "reactome_id": "R-HSA-66000", "label": "TXN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8866399", "reactome_id": "R-ALL-8866399", "label": "dNDP(3-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73316", "formula": "C5H8O9P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-73668", "reactome_id": "R-HSA-73668", "label": "2xHC-TXN", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor of RNR (M1M2B) [cytosol]", "activity_id": "111796", "ec_numbers": [ "1.17.4.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11796141", "title": "Structure and function of the radical enzyme ribonucleotide reductase", "journal": "Prog Biophys Mol Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11796141/" }, { "id": "PMID:11517226", "title": "Mammalian p53R2 protein forms an active ribonucleotide reductase in vitro with the R1 protein, which is expressed both in resting cells in response to DNA damage and in proliferating cells", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11517226/" }, { "id": "PMID:2668278", "title": "Thioredoxin and glutaredoxin systems", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2668278/" }, { "id": "PMID:18997010", "title": "Ribonucleotide reduction is a cytosolic process in mammalian cells independently of DNA damage", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18997010/" }, { "id": "PMID:22847445", "title": "Mammalian ribonucleotide reductase subunit p53R2 is required for mitochondrial DNA replication and DNA repair in quiescent cells", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22847445/" }, { "id": "PMID:10884394", "title": "Cross-talk between the allosteric effector-binding sites in mouse ribonucleotide reductase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10884394/" } ], "x": 3600, "y": 3160.8541666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-111883", "label": "PLA2G4A (cPLA2) hydrolyzes phosphatidylcholine", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005829", "GO:0005788" ], "rhea_ids": [ "RHEA:40652", "RHEA:40651" ], "source_url": "https://reactome.org/content/detail/R-HSA-111883", "summary": "PLA2G4A (cPLA2) hydrolyzes phosphatidylcholine is a Reactome release 97 curated reaction event. The source record connects H2O, AA-PC to AA, H+, LysoPtdCho in endoplasmic reticulum membrane · cytosol · endoplasmic reticulum lumen and cross-references RHEA:40652, RHEA:40651.", "equation": "H2O + AA-PC → AA + H+ + LysoPtdCho", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9915525", "reactome_id": "R-ALL-9915525", "label": "AA-PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:75063", "formula": "C29H49NO8PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-6814253", "reactome_id": "R-ALL-6814253", "label": "LysoPtdCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58168", "formula": "C9H19NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of Active PLA2:phosphatidylcholine [endoplasmic reticulum membrane]", "activity_id": "111882", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:21247147", "title": "Functional characterization of mutations in inherited human cPLA? deficiency", "journal": "Biochemistry", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21247147/" }, { "id": "PMID:21746768", "title": "Secreted phospholipase A2 revisited", "journal": "J. Biochem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21746768/" } ], "x": 590, "y": 1543.561403508772, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-112033", "label": "PNP catalyzes the conversion of hypoxanthine and (deoxy)ribose-1-phosphate to (deoxy)inosine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27648", "RHEA:27752" ], "source_url": "https://reactome.org/content/detail/R-HSA-112033", "summary": "PNP catalyzes the conversion of hypoxanthine and (deoxy)ribose-1-phosphate to (deoxy)inosine is a Reactome release 97 curated reaction event. The source record connects Hyp, R1P, dRibP to Pi, Ino, dI in cytosol and cross-references RHEA:27648, RHEA:27752.", "equation": "Hyp + R1P, dRibP → Pi + Ino, dI", "inputs": [ { "node_id": "R-ALL-113599", "reactome_id": "R-ALL-113599", "label": "Hyp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17368", "formula": "C5H4N4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500244", "reactome_id": "R-ALL-500244", "label": "R1P, dRibP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500172", "reactome_id": "R-ALL-500172", "label": "Ino, dI", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "purine-nucleoside phosphorylase activity of PNP trimer [cytosol]", "activity_id": "74238", "ec_numbers": [ "2.4.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6771276", "title": "Characterization of purine nucleoside phosphorylase from human granulocytes and its metabolism of deoxyribonucleosides", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6771276/" }, { "id": "PMID:3029074", "title": "A human purine nucleoside phosphorylase deficiency caused by a single base change", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3029074/" }, { "id": "PMID:2104852", "title": "Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 A resolution", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2104852/" } ], "x": 3600, "y": 2835.035714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-112034", "label": "PNP catalyzes the conversion of guanine and (deoxy)ribose-1-phosphate to (deoxy)guanosine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13235", "RHEA:27740" ], "source_url": "https://reactome.org/content/detail/R-HSA-112034", "summary": "PNP catalyzes the conversion of guanine and (deoxy)ribose-1-phosphate to (deoxy)guanosine is a Reactome release 97 curated reaction event. The source record connects Gua, R1P, dRibP to Pi, G, dG in cytosol and cross-references RHEA:13235, RHEA:27740.", "equation": "Gua + R1P, dRibP → Pi + G, dG", "inputs": [ { "node_id": "R-ALL-83956", "reactome_id": "R-ALL-83956", "label": "Gua", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16235", "formula": "C5H5N5O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500244", "reactome_id": "R-ALL-500244", "label": "R1P, dRibP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500245", "reactome_id": "R-ALL-500245", "label": "G, dG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "purine-nucleoside phosphorylase activity of PNP trimer [cytosol]", "activity_id": "74238", "ec_numbers": [ "2.4.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6771276", "title": "Characterization of purine nucleoside phosphorylase from human granulocytes and its metabolism of deoxyribonucleosides", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6771276/" }, { "id": "PMID:3029074", "title": "A human purine nucleoside phosphorylase deficiency caused by a single base change", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3029074/" }, { "id": "PMID:2104852", "title": "Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 A resolution", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2104852/" } ], "x": 3878.666666666667, "y": 2899.1071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-112265", "label": "thymidine or deoxyuridine + orthophosphate thymine or uracil + 2-deoxy-D-ribose 1-phosphate [TYMP]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16038", "RHEA:22825" ], "source_url": "https://reactome.org/content/detail/R-HSA-112265", "summary": "thymidine or deoxyuridine + orthophosphate thymine or uracil + 2-deoxy-D-ribose 1-phosphate [TYMP] is a Reactome release 97 curated reaction event. The source record connects Thy-Rib, dU, Pi to Thy, Ura, dRibP in cytosol and cross-references RHEA:16038, RHEA:22825.", "equation": "Thy-Rib, dU + Pi → Thy, Ura + dRibP", "inputs": [ { "node_id": "R-ALL-500444", "reactome_id": "R-ALL-500444", "label": "Thy-Rib, dU", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-500438", "reactome_id": "R-ALL-500438", "label": "Thy, Ura", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-112032", "reactome_id": "R-ALL-112032", "label": "dRibP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58576", "formula": "C5H9O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pentosyltransferase activity of TYMP dimer [cytosol]", "activity_id": "73611", "ec_numbers": [ "2.4.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:7284378", "title": "Catabolism of thymidine in human blood platelets: purification and properties of thymidine phosphorylase", "journal": "Biochim Biophys Acta", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/7284378/" }, { "id": "PMID:14725767", "title": "Crystal structure of human thymidine phosphorylase in complex with a small molecule inhibitor", "journal": "Structure", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14725767/" }, { "id": "PMID:1590793", "title": "Platelet-derived endothelial cell growth factor has thymidine phosphorylase activity.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1590793/" } ], "x": 2381.3333333333335, "y": 3174.2023809523807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-112266", "label": "thymine or uracil + 2-deoxy-D-ribose 1-phosphate thymidine or deoxyuridine + orthophosphate [TYMP]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16039", "RHEA:22826" ], "source_url": "https://reactome.org/content/detail/R-HSA-112266", "summary": "thymine or uracil + 2-deoxy-D-ribose 1-phosphate thymidine or deoxyuridine + orthophosphate [TYMP] is a Reactome release 97 curated reaction event. The source record connects Thy, Ura, dRibP to Thy-Rib, dU, Pi in cytosol and cross-references RHEA:16039, RHEA:22826.", "equation": "Thy, Ura + dRibP → Thy-Rib, dU + Pi", "inputs": [ { "node_id": "R-ALL-500438", "reactome_id": "R-ALL-500438", "label": "Thy, Ura", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-112032", "reactome_id": "R-ALL-112032", "label": "dRibP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58576", "formula": "C5H9O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-500444", "reactome_id": "R-ALL-500444", "label": "Thy-Rib, dU", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pentosyltransferase activity of TYMP dimer [cytosol]", "activity_id": "73611", "ec_numbers": [ "2.4.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:7284378", "title": "Catabolism of thymidine in human blood platelets: purification and properties of thymidine phosphorylase", "journal": "Biochim Biophys Acta", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/7284378/" }, { "id": "PMID:14725767", "title": "Crystal structure of human thymidine phosphorylase in complex with a small molecule inhibitor", "journal": "Structure", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14725767/" }, { "id": "PMID:1590793", "title": "Platelet-derived endothelial cell growth factor has thymidine phosphorylase activity.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1590793/" } ], "x": 3321.3333333333335, "y": 2920.464285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1237045", "label": "Carbonic Anhydrase VI hydrates carbon dioxide to bicarbonate and a proton", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "carbon-dioxide" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [ "RHEA:10750" ], "source_url": "https://reactome.org/content/detail/R-HSA-1237045", "summary": "Carbonic Anhydrase VI hydrates carbon dioxide to bicarbonate and a proton is a Reactome release 97 curated reaction event. The source record connects CO2, H2O to H+, HCO3- in extracellular region and cross-references RHEA:10750.", "equation": "CO2 + H2O → H+ + HCO3-", "inputs": [ { "node_id": "R-ALL-1237009", "reactome_id": "R-ALL-1237009", "label": "CO2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-425425", "reactome_id": "R-ALL-425425", "label": "HCO3-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbonate dehydratase activity of CA6:Zinc [extracellular region]", "activity_id": "1237060", "ec_numbers": [ "4.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9784398", "title": "Gustin from human parotid saliva is carbonic anhydrase VI", "journal": "Biochem Biophys Res Commun", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9784398/" }, { "id": "PMID:17499996", "title": "Carbonic anhydrase activators: the first activation study of the human secretory isoform VI with amino acids and amines", "journal": "Bioorg Med Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17499996/" }, { "id": "PMID:17228881", "title": "Carbonic anhydrase inhibitors. DNA cloning, characterization, and inhibition studies of the human secretory isoform VI, a new target for sulfonamide and sulfamate inhibitors", "journal": "J Med Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17228881/" }, { "id": "PMID:9336012", "title": "Structure and mechanism of carbonic anhydrase", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9336012/" } ], "x": 6521.333333333333, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1237081", "label": "Carbonic anhydrase VI dehydrates bicarbonate to water and carbon dioxide", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "carbon-dioxide" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [ "RHEA:10749" ], "source_url": "https://reactome.org/content/detail/R-HSA-1237081", "summary": "Carbonic anhydrase VI dehydrates bicarbonate to water and carbon dioxide is a Reactome release 97 curated reaction event. The source record connects H+, HCO3- to CO2, H2O in extracellular region and cross-references RHEA:10749.", "equation": "H+ + HCO3- → CO2 + H2O", "inputs": [ { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-425425", "reactome_id": "R-ALL-425425", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1237009", "reactome_id": "R-ALL-1237009", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbonate dehydratase activity of CA6:Zinc [extracellular region]", "activity_id": "1237060", "ec_numbers": [ "4.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9784398", "title": "Gustin from human parotid saliva is carbonic anhydrase VI", "journal": "Biochem Biophys Res Commun", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9784398/" }, { "id": "PMID:17499996", "title": "Carbonic anhydrase activators: the first activation study of the human secretory isoform VI with amino acids and amines", "journal": "Bioorg Med Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17499996/" }, { "id": "PMID:17228881", "title": "Carbonic anhydrase inhibitors. DNA cloning, characterization, and inhibition studies of the human secretory isoform VI, a new target for sulfonamide and sulfamate inhibitors", "journal": "J Med Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17228881/" }, { "id": "PMID:9336012", "title": "Structure and mechanism of carbonic anhydrase", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9336012/" } ], "x": 6800, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1237096", "label": "Methylthio-ribose-P =\u003e Methylthio-ribulose-P", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19990" ], "source_url": "https://reactome.org/content/detail/R-HSA-1237096", "summary": "Methylthio-ribose-P =\u003e Methylthio-ribulose-P is a Reactome release 97 curated reaction event. The source record connects MTRIBP to MTRIBUP in cytosol and cross-references RHEA:19990.", "equation": "MTRIBP → MTRIBUP", "inputs": [ { "node_id": "R-ALL-1237165", "reactome_id": "R-ALL-1237165", "label": "MTRIBP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58533", "formula": "C6H11O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1237107", "reactome_id": "R-ALL-1237107", "label": "MTRIBUP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58548", "formula": "C6H11O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "S-methyl-5-thioribose-1-phosphate isomerase activity of MRI1 [cytosol]", "activity_id": "1237154", "ec_numbers": [ "5.3.1.23" ], "accessions": [ "Q9BV20" ] } ], "citations": [ { "id": "PMID:19620624", "title": "A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme", "journal": "Mol Cell Proteomics", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19620624/" } ], "x": 1251.3333333333333, "y": 2965.4444444444443, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1237102", "label": "Transamination of MOB to methionine", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:31765" ], "source_url": "https://reactome.org/content/detail/R-HSA-1237102", "summary": "Transamination of MOB to methionine is a Reactome release 97 curated reaction event. The source record connects 4MTOBUTA, L-amino acid to L-Met, 2-Oxoacid in cytosol and cross-references RHEA:31765.", "equation": "4MTOBUTA + L-amino acid → L-Met + 2-Oxoacid", "inputs": [ { "node_id": "R-ALL-1237122", "reactome_id": "R-ALL-1237122", "label": "4MTOBUTA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16723", "formula": "C5H7O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8942986", "reactome_id": "R-ALL-8942986", "label": "L-amino acid", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59869", "formula": "C2H4NO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-174390", "reactome_id": "R-ALL-174390", "label": "L-Met", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57844", "formula": "C5H11NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1237175", "reactome_id": "R-ALL-1237175", "label": "2-Oxoacid", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35179", "formula": "C2O3R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transaminase activity of PXLP-K259-GOT1 dimer [cytosol]", "activity_id": "1295495", "ec_numbers": [ "2.6.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11443076", "title": "Methionine regeneration and aspartate aminotransferase in parasitic protozoa", "journal": "J Bacteriol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11443076/" } ], "x": 1808.6666666666667, "y": 2850.777777777778, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1237119", "label": "Acireductone is oxidized to MOB", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24505" ], "source_url": "https://reactome.org/content/detail/R-HSA-1237119", "summary": "Acireductone is oxidized to MOB is a Reactome release 97 curated reaction event. The source record connects Acireductone, O2 to 4MTOBUTA, HCOOH, H+ in cytosol and cross-references RHEA:24505.", "equation": "Acireductone + O2 → 4MTOBUTA + HCOOH + 2 H+", "inputs": [ { "node_id": "R-ALL-1237155", "reactome_id": "R-ALL-1237155", "label": "Acireductone", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:49252", "formula": "C6H10O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1237122", "reactome_id": "R-ALL-1237122", "label": "4MTOBUTA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16723", "formula": "C5H7O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acireductone dioxygenase [iron(II)-requiring] activity of ARD:Fe++ [cytosol]", "activity_id": "1237139", "ec_numbers": [ "1.13.11.54" ], "accessions": [] } ], "citations": [ { "id": "PMID:17786183", "title": "Expression and function of the human androgen-responsive gene ADI1 in prostate cancer", "journal": "Neoplasia", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17786183/" } ], "x": 1530, "y": 2831.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1237129", "label": "Acireductone is created", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21701" ], "source_url": "https://reactome.org/content/detail/R-HSA-1237129", "summary": "Acireductone is created is a Reactome release 97 curated reaction event. The source record connects 2,3-DMPP, H2O to Acireductone, Pi in cytosol and cross-references RHEA:21701.", "equation": "2,3-DMPP + H2O → Acireductone + Pi", "inputs": [ { "node_id": "R-ALL-1237176", "reactome_id": "R-ALL-1237176", "label": "2,3-DMPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58828", "formula": "C6H9O6PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1237155", "reactome_id": "R-ALL-1237155", "label": "Acireductone", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:49252", "formula": "C6H10O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acireductone synthase activity of E1:Mg++ [cytosol]", "activity_id": "1237084", "ec_numbers": [ "3.1.3.77" ], "accessions": [] } ], "citations": [ { "id": "PMID:15843022", "title": "Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity", "journal": "J Mol Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15843022/" } ], "x": 1251.3333333333333, "y": 2869.8888888888887, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1237140", "label": "Dehydration of methylthio-ribulose-P", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15550" ], "source_url": "https://reactome.org/content/detail/R-HSA-1237140", "summary": "Dehydration of methylthio-ribulose-P is a Reactome release 97 curated reaction event. The source record connects MTRIBUP to 2,3-DMPP, H2O in cytosol and cross-references RHEA:15550.", "equation": "MTRIBUP → 2,3-DMPP + H2O", "inputs": [ { "node_id": "R-ALL-1237107", "reactome_id": "R-ALL-1237107", "label": "MTRIBUP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58548", "formula": "C6H11O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1237176", "reactome_id": "R-ALL-1237176", "label": "2,3-DMPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58828", "formula": "C6H9O6PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "methylthioribulose 1-phosphate dehydratase activity of APIP:Zn++ [cytosol]", "activity_id": "1237163", "ec_numbers": [ "4.2.1.109" ], "accessions": [] } ], "citations": [], "x": 1808.6666666666667, "y": 2946.3333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1237160", "label": "MTA is cleaved and phosphorylated", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11853" ], "source_url": "https://reactome.org/content/detail/R-HSA-1237160", "summary": "MTA is cleaved and phosphorylated is a Reactome release 97 curated reaction event. The source record connects MTAD, Pi to MTRIBP, Ade in cytosol and cross-references RHEA:11853.", "equation": "MTAD + Pi → MTRIBP + Ade", "inputs": [ { "node_id": "R-ALL-353106", "reactome_id": "R-ALL-353106", "label": "MTAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17509", "formula": "C11H15N5O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1237165", "reactome_id": "R-ALL-1237165", "label": "MTRIBP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58533", "formula": "C6H11O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-83951", "reactome_id": "R-ALL-83951", "label": "Ade", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16708", "formula": "C5H5N5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "S-methyl-5-thioadenosine phosphorylase activity of MTAP trimer [cytosol]", "activity_id": "1237143", "ec_numbers": [ "2.4.2.28" ], "accessions": [] } ], "citations": [ { "id": "PMID:6785752", "title": "Selective killing of human malignant cell lines deficient in methylthioadenosine phosphorylase, a purine metabolic enzyme", "journal": "Proc Natl Acad Sci U S A", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/6785752/" }, { "id": "PMID:3091600", "title": "Purification and characterization of 5'-deoxy-5'-methylthioadenosine phosphorylase from human placenta", "journal": "J Biol Chem", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3091600/" }, { "id": "PMID:22464254", "title": "Primate Genome Gain and Loss: A Bone Dysplasia, Muscular Dystrophy, and Bone Cancer Syndrome Resulting from Mutated Retroviral-Derived MTAP Transcripts", "journal": "Am J Hum Genet", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22464254/" } ], "x": 1530, "y": 2869.8888888888887, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1247910", "label": "CNDP2:2Mn2+ dimer hydrolyses CysGly", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:28784" ], "source_url": "https://reactome.org/content/detail/R-HSA-1247910", "summary": "CNDP2:2Mn2+ dimer hydrolyses CysGly is a Reactome release 97 curated reaction event. The source record connects CysGly, H2O to L-Cys, Gly in cytosol and cross-references RHEA:28784.", "equation": "CysGly + H2O → L-Cys + Gly", "inputs": [ { "node_id": "R-ALL-1247904", "reactome_id": "R-ALL-1247904", "label": "CysGly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61694", "formula": "C5H10N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carboxypeptidase activity of CNDP2:2Mn2+ dimer [cytosol]", "activity_id": "1258416", "ec_numbers": [ "3.4.17.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:12473676", "title": "Sequence identification and characterization of human carnosinase and a closely related non-specific dipeptidase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12473676/" } ], "x": 311.33333333333337, "y": 3556.333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1247915", "label": "GSH translocates from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:74819" ], "source_url": "https://reactome.org/content/detail/R-HSA-1247915", "summary": "GSH translocates from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects GSH to GSH in plasma membrane and cross-references RHEA:74819.", "equation": "GSH → GSH", "inputs": [ { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1247892", "reactome_id": "R-ALL-1247892", "label": "GSH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16856", "formula": "C10H17N3O6S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:34150", "title": "Translocation of intracellular glutathione to membrane-bound gamma-glutamyl transpeptidase as a discrete step in the gamma-glutamyl cycle: glutathionuria after inhibition of transpeptidase", "journal": "Proc Natl Acad Sci U S A", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/34150/" }, { "id": "PMID:42913", "title": "Transport of gamma-glutamyl amino acids: role of glutathione and gamma-glutamyl transpeptidase", "journal": "Proc Natl Acad Sci U S A", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/42913/" } ], "x": 311.33333333333337, "y": 3632.777777777778, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1247922", "label": "GGCT transforms gGluCys to OPRO", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1247922", "summary": "GGCT transforms gGluCys to OPRO is a Reactome release 97 curated reaction event. The source record connects gGluCys to L-Cys, OPRO in cytosol.", "equation": "gGluCys → L-Cys + OPRO", "inputs": [ { "node_id": "R-ALL-174393", "reactome_id": "R-ALL-174393", "label": "gGluCys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58173", "formula": "C8H13N2O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1247942", "reactome_id": "R-ALL-1247942", "label": "OPRO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58402", "formula": "C5H6NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "gamma-glutamylcyclotransferase activity of GGCT dimer [cytosol]", "activity_id": "1247932", "ec_numbers": [ "4.3.2.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:18515354", "title": "The identification and structural characterization of C7orf24 as gamma-glutamyl cyclotransferase. An essential enzyme in the gamma-glutamyl cycle", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18515354/" } ], "x": 590, "y": 3632.777777777778, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1247935", "label": "OPLAH hydrolyses OPRO to L-Glu", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10349" ], "source_url": "https://reactome.org/content/detail/R-HSA-1247935", "summary": "OPLAH hydrolyses OPRO to L-Glu is a Reactome release 97 curated reaction event. The source record connects ATP, H2O, OPRO to ADP, L-Glu, Pi in cytosol and cross-references RHEA:10349.", "equation": "ATP + 2 H2O + OPRO → ADP + L-Glu + Pi", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1247942", "reactome_id": "R-ALL-1247942", "label": "OPRO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58402", "formula": "C5H6NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5-oxoprolinase (ATP-hydrolyzing) activity of OPLAH [cytosol]", "activity_id": "1247941", "ec_numbers": [ "3.5.2.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:9516961", "title": "Characterization of 5-oxo-L-prolinase in normal and tumor tissues of humans and rats: a potential new target for biochemical modulation of glutathione", "journal": "Clin Cancer Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9516961/" } ], "x": 311.33333333333337, "y": 3479.8888888888887, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1247939", "label": "CysGly translocates from extracellular region to cytosol", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1247939", "summary": "CysGly translocates from extracellular region to cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CysGly to CysGly in plasma membrane.", "equation": "CysGly → CysGly", "inputs": [ { "node_id": "R-ALL-1247934", "reactome_id": "R-ALL-1247934", "label": "CysGly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61694", "formula": "C5H10N2O3S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1247904", "reactome_id": "R-ALL-1247904", "label": "CysGly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61694", "formula": "C5H10N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:31622", "title": "Evidence that the gamma-glutamyl cycle functions in vivo using intracellular glutathione: effects of amino acids and selective inhibition of enzymes", "journal": "Proc Natl Acad Sci U S A", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/31622/" } ], "x": 868.6666666666667, "y": 3632.777777777778, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1299507", "label": "Methylthio-ribulose-P =\u003e Methylthio-ribose-P", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19991" ], "source_url": "https://reactome.org/content/detail/R-HSA-1299507", "summary": "Methylthio-ribulose-P =\u003e Methylthio-ribose-P is a Reactome release 97 curated reaction event. The source record connects MTRIBUP to MTRIBP in cytosol and cross-references RHEA:19991.", "equation": "MTRIBUP → MTRIBP", "inputs": [ { "node_id": "R-ALL-1237107", "reactome_id": "R-ALL-1237107", "label": "MTRIBUP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58548", "formula": "C6H11O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1237165", "reactome_id": "R-ALL-1237165", "label": "MTRIBP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58533", "formula": "C6H11O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "S-methyl-5-thioribose-1-phosphate isomerase activity of MRI1 [cytosol]", "activity_id": "1237154", "ec_numbers": [ "5.3.1.23" ], "accessions": [ "Q9BV20" ] } ], "citations": [ { "id": "PMID:19620624", "title": "A mediator of Rho-dependent invasion moonlights as a methionine salvage enzyme", "journal": "Mol Cell Proteomics", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19620624/" } ], "x": 1530, "y": 2965.4444444444443, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1362408", "label": "FXN:NFS1:ISD11:ISCU assembles 2Fe-2S iron-sulfur cluster", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "iron-sulfur" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1362408", "summary": "FXN:NFS1:ISD11:ISCU assembles 2Fe-2S iron-sulfur cluster is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 2 Iron:FXN:NFS1:ISD11:ISCU, L-Cys, FDX1,FDX2 (reduced) to FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster, L-Ala, FDX1,FDX2 (oxidized) in mitochondrial matrix.", "equation": "2 Iron:FXN:NFS1:ISD11:ISCU + 4 L-Cys + 4 FDX1,FDX2 (reduced) → FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster + 4 L-Ala + 4 FDX1,FDX2 (oxidized)", "inputs": [ { "node_id": "R-HSA-1362401", "reactome_id": "R-HSA-1362401", "label": "2 Iron:FXN:NFS1:ISD11:ISCU", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-379721", "reactome_id": "R-ALL-379721", "label": "L-Cys", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2408372", "reactome_id": "R-HSA-2408372", "label": "FDX1,FDX2 (reduced)", "role": "input", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1362398", "reactome_id": "R-HSA-1362398", "label": "FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-379697", "reactome_id": "R-ALL-379697", "label": "L-Ala", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2408370", "reactome_id": "R-HSA-2408370", "label": "FDX1,FDX2 (oxidized)", "role": "output", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "cysteine desulfurase activity of 2 Iron:FXN:NFS1:ISD11:ISCU [mitochondrial matrix]", "activity_id": "1362414", "ec_numbers": [ "2.8.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:20873749", "title": "Human frataxin is an allosteric switch that activates the Fe-S cluster biosynthetic complex", "journal": "Biochemistry", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20873749/" }, { "id": "PMID:19454487", "title": "Human ISD11 is essential for both iron-sulfur cluster assembly and maintenance of normal cellular iron homeostasis", "journal": "Hum Mol Genet", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19454487/" }, { "id": "PMID:17331979", "title": "Mitochondrial frataxin interacts with ISD11 of the NFS1/ISCU complex and multiple mitochondrial chaperones", "journal": "Hum Mol Genet", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17331979/" }, { "id": "PMID:12785837", "title": "Iron-sulfur cluster biosynthesis. Characterization of frataxin as an iron donor for assembly of [2Fe-2S] clusters in ISU-type proteins", "journal": "J Am Chem Soc", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12785837/" }, { "id": "PMID:22352884", "title": "Effector role reversal during evolution: the case of frataxin in Fe-S cluster biosynthesis", "journal": "Biochemistry", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22352884/" }, { "id": "PMID:22101253", "title": "Both human ferredoxins 1 and 2 and ferredoxin reductase are important for iron-sulfur cluster biogenesis", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22101253/" } ], "x": 5948.666666666667, "y": 3573.75, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-1362416", "label": "Frataxin binds iron", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "iron-sulfur" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1362416", "summary": "Frataxin binds iron is a Reactome release 97 curated reaction event. The source record connects FXN:ISD11:NFS1:ISCU, Fe2+ to 2 Iron:FXN:NFS1:ISD11:ISCU in mitochondrial matrix.", "equation": "FXN:ISD11:NFS1:ISCU + 4 Fe2+ → 2 Iron:FXN:NFS1:ISD11:ISCU", "inputs": [ { "node_id": "R-HSA-1362404", "reactome_id": "R-HSA-1362404", "label": "FXN:ISD11:NFS1:ISCU", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113531", "reactome_id": "R-ALL-113531", "label": "Fe2+", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1362401", "reactome_id": "R-HSA-1362401", "label": "2 Iron:FXN:NFS1:ISD11:ISCU", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "ferrous iron binding activity of FXN:ISD11:NFS1:ISCU [mitochondrial matrix]", "activity_id": "9954151", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:20873749", "title": "Human frataxin is an allosteric switch that activates the Fe-S cluster biosynthetic complex", "journal": "Biochemistry", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20873749/" }, { "id": "PMID:21298097", "title": "Mammalian frataxin: an essential function for cellular viability through an interaction with a preformed ISCU/NFS1/ISD11 iron-sulfur assembly complex", "journal": "PLoS One", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21298097/" }, { "id": "PMID:12785837", "title": "Iron-sulfur cluster biosynthesis. Characterization of frataxin as an iron donor for assembly of [2Fe-2S] clusters in ISU-type proteins", "journal": "J Am Chem Soc", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12785837/" }, { "id": "PMID:18425540", "title": "Mapping iron binding sites on human frataxin: implications for cluster assembly on the ISU Fe-S cluster scaffold protein", "journal": "J Biol Inorg Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18425540/" }, { "id": "PMID:11823441", "title": "Assembly and iron-binding properties of human frataxin, the protein deficient in Friedreich ataxia", "journal": "Hum Mol Genet", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11823441/" }, { "id": "PMID:21776984", "title": "Structure-function analysis of Friedreich's ataxia mutants reveals determinants of frataxin binding and activation of the Fe-S assembly complex", "journal": "Biochemistry", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21776984/" } ], "x": 5948.666666666667, "y": 3379.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1362417", "label": "Mitoferrin translocates iron from the mitochondrial intermembrane space to the mitochondrial matrix", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "iron-sulfur" ], "compartment": "mitochondrial inner membrane · mitochondrial intermembrane space · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005758", "GO:0005759" ], "rhea_ids": [ "RHEA:28486" ], "source_url": "https://reactome.org/content/detail/R-HSA-1362417", "summary": "Mitoferrin translocates iron from the mitochondrial intermembrane space to the mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects Fe2+ to Fe2+ in mitochondrial inner membrane · mitochondrial intermembrane space · mitochondrial matrix and cross-references RHEA:28486.", "equation": "Fe2+ → Fe2+", "inputs": [ { "node_id": "R-ALL-1362385", "reactome_id": "R-ALL-1362385", "label": "Fe2+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113531", "reactome_id": "R-ALL-113531", "label": "Fe2+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ferrous iron transmembrane transporter activity of Mitoferrin1,2 [mitochondrial inner membrane]", "activity_id": "1362410", "ec_numbers": null, "accessions": [] } ], "citations": [], "x": 5391.333333333333, "y": 3379.25, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-139970", "label": "FMO3:FAD N-oxidises TMA to TMAO", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:31980" ], "source_url": "https://reactome.org/content/detail/R-HSA-139970", "summary": "FMO3:FAD N-oxidises TMA to TMAO is a Reactome release 97 curated reaction event. The source record connects TMA, O2, NADPH to TMAO, H2O, NADP+ in endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:31980.", "equation": "TMA + O2 + NADPH + H+ → TMAO + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-30373", "reactome_id": "R-ALL-30373", "label": "TMA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18139", "formula": "C3H9N", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-140963", "reactome_id": "R-ALL-140963", "label": "TMAO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15724", "formula": "C3H9NO", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N,N-dimethylaniline monooxygenase activity of FMO3:FAD [endoplasmic reticulum membrane]", "activity_id": "114712", "ec_numbers": [ "1.14.13.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:5048998", "title": "Trimethylamine N-oxide synthesis: a human variant", "journal": "Biochem Med", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/5048998/" }, { "id": "PMID:4195988", "title": "Trimethylaminuria: the fish-odour syndrome", "journal": "Lancet", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/4195988/" }, { "id": "PMID:9536088", "title": "Mutations of the flavin-containing monooxygenase gene (FMO3) cause trimethylaminuria, a defect in detoxication", "journal": "Hum. Mol. Genet.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9536088/" }, { "id": "PMID:23563705", "title": "Intestinal microbiota metabolism of L-carnitine, a nutrient in red meat, promotes atherosclerosis", "journal": "Nat. Med.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23563705/" } ], "x": 7740, "y": 2814.4411764705883, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-140355", "label": "Arachidonate is oxidised to PGG2 by PTGS1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "biological-oxidations", "fatty-acid-metabolism", "phase-one" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:42597" ], "source_url": "https://reactome.org/content/detail/R-HSA-140355", "summary": "Arachidonate is oxidised to PGG2 by PTGS1 is a Reactome release 97 curated reaction event. The source record connects O2, AA to PGG2 in endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:42597.", "equation": "2 O2 + AA → PGG2", "inputs": [ { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-140357", "reactome_id": "R-ALL-140357", "label": "PGG2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:82629", "formula": "C20H31O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "prostaglandin-endoperoxide synthase activity of PTGS1 dimer [endoplasmic reticulum membrane]", "activity_id": "140354", "ec_numbers": [ "1.14.99.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:4521806", "title": "Isolation and structure of two prostaglandin endoperoxides that cause platelet aggregation", "journal": "Proc Natl Acad Sci U S A", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4521806/" }, { "id": "PMID:4776443", "title": "Isolation and properties of intermediates in prostaglandin biosynthesis", "journal": "Biochim Biophys Acta", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4776443/" } ], "x": 311.33333333333337, "y": 1597.877192982456, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-140359", "label": "PGG2 is reduced to PGH2 by PTGS1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "biological-oxidations", "fatty-acid-metabolism", "phase-one" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:42601", "RHEA:42600" ], "source_url": "https://reactome.org/content/detail/R-HSA-140359", "summary": "PGG2 is reduced to PGH2 by PTGS1 is a Reactome release 97 curated reaction event. The source record connects PGG2, hydrogen donor to H2O, PGH2, hydrogen acceptor in endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:42601, RHEA:42600.", "equation": "PGG2 + hydrogen donor → H2O + PGH2 + hydrogen acceptor", "inputs": [ { "node_id": "R-ALL-140357", "reactome_id": "R-ALL-140357", "label": "PGG2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:82629", "formula": "C20H31O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9915557", "reactome_id": "R-ALL-9915557", "label": "hydrogen donor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17499", "formula": "H2A", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30138", "reactome_id": "R-ALL-30138", "label": "PGH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9915551", "reactome_id": "R-ALL-9915551", "label": "hydrogen acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13193", "formula": "A", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "peroxidase activity of PTGS1 dimer [endoplasmic reticulum membrane]", "activity_id": "140358", "ec_numbers": [ "1.11.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:4514999", "title": "Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis", "journal": "Proc Natl Acad Sci U S A", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4514999/" }, { "id": "PMID:1380156", "title": "Human cyclooxygenase-2 cDNA", "journal": "Proc Natl Acad Sci U S A", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1380156/" }, { "id": "PMID:9139685", "title": "Differential allosteric regulation of prostaglandin H synthase 1 and 2 by arachidonic acid", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9139685/" }, { "id": "PMID:7947975", "title": "Purification, characterization and selective inhibition of human prostaglandin G/H synthase 1 and 2 expressed in the baculovirus system", "journal": "Biochim Biophys Acta", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7947975/" } ], "x": 868.6666666666667, "y": 1543.561403508772, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-141186", "label": "MAOA:FAD oxidatively deaminates of 5HT", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:69073" ], "source_url": "https://reactome.org/content/detail/R-HSA-141186", "summary": "MAOA:FAD oxidatively deaminates of 5HT is a Reactome release 97 curated reaction event. The source record connects O2, H2O, 5HT to 5HIALD, H2O2, NH4+ in cytosol · mitochondrial outer membrane and cross-references RHEA:69073.", "equation": "O2 + H2O + 5HT → 5HIALD + H2O2 + NH4+", "inputs": [ { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-209914", "reactome_id": "R-ALL-209914", "label": "5HT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:350546", "formula": "C10H13N2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-500604", "reactome_id": "R-ALL-500604", "label": "5HIALD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:50157", "formula": "C10H9NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "primary methylamine oxidase activity of MAOA:FAD [mitochondrial outer membrane]", "activity_id": "141714", "ec_numbers": [ "1.4.3.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:6408492", "title": "The deamination of dopamine by human brain monoamine oxidase. Specificity for the two enzyme forms in seven brain regions", "journal": "Naunyn Schmiedebergs Arch Pharmacol", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6408492/" }, { "id": "PMID:2764901", "title": "Monoamine oxidase A from human placenta and monoamine oxidase B from bovine liver both have one FAD per subunit", "journal": "Biochem. J.", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2764901/" } ], "x": 7740, "y": 2829.6960784313724, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-141200", "label": "MAOB:FAD oxidatively deaminates of PEA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:25266" ], "source_url": "https://reactome.org/content/detail/R-HSA-141200", "summary": "MAOB:FAD oxidatively deaminates of PEA is a Reactome release 97 curated reaction event. The source record connects PEA, H2O, O2 to Phenylacetaldehyde, NH3, H2O2 in cytosol · mitochondrial outer membrane and cross-references RHEA:25266.", "equation": "PEA + H2O + O2 → Phenylacetaldehyde + NH3 + H2O2", "inputs": [ { "node_id": "R-ALL-500605", "reactome_id": "R-ALL-500605", "label": "PEA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18397", "formula": "C8H11N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-500607", "reactome_id": "R-ALL-500607", "label": "Phenylacetaldehyde", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16424", "formula": "C8H8O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29382", "reactome_id": "R-ALL-29382", "label": "NH3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16134", "formula": "H3N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "primary methylamine oxidase activity of MAOB:FAD [mitochondrial outer membrane]", "activity_id": "141195", "ec_numbers": [ "1.4.3.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:6788990", "title": "Studies on beta-phenylethylamine deamination by human placental monoamine oxidase", "journal": "Jpn J Pharmacol", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/6788990/" } ], "x": 8018.666666666667, "y": 2829.6960784313724, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-141202", "label": "MAOB:FAD oxidatively deaminates TYR", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:30592" ], "source_url": "https://reactome.org/content/detail/R-HSA-141202", "summary": "MAOB:FAD oxidatively deaminates TYR is a Reactome release 97 curated reaction event. The source record connects TYR, H2O, O2 to HPHAC, NH3, H2O2 in cytosol · mitochondrial outer membrane and cross-references RHEA:30592.", "equation": "TYR + H2O + O2 → HPHAC + NH3 + H2O2", "inputs": [ { "node_id": "R-ALL-500606", "reactome_id": "R-ALL-500606", "label": "TYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15760", "formula": "C8H11NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-500608", "reactome_id": "R-ALL-500608", "label": "HPHAC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15621", "formula": "C8H8O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29382", "reactome_id": "R-ALL-29382", "label": "NH3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16134", "formula": "H3N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "primary methylamine oxidase activity of MAOB:FAD [mitochondrial outer membrane]", "activity_id": "141195", "ec_numbers": [ "1.4.3.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:4016087", "title": "Human brain monoamine oxidase type B: mechanism of deamination as probed by steady-state methods", "journal": "Biochemistry", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/4016087/" } ], "x": 7461.333333333333, "y": 2837.323529411765, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-141341", "label": "SMOX-3 oxidises SPN to SPM", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "peroxisomal matrix · cytosol", "compartment_ids": [ "GO:0005782", "GO:0005829" ], "rhea_ids": [ "RHEA:25805" ], "source_url": "https://reactome.org/content/detail/R-HSA-141341", "summary": "SMOX-3 oxidises SPN to SPM is a Reactome release 97 curated reaction event. The source record connects SPN, H2O, O2 to SPM, 3APAL, H2O2 in peroxisomal matrix · cytosol and cross-references RHEA:25805.", "equation": "SPN + H2O + O2 → SPM + 3APAL + H2O2", "inputs": [ { "node_id": "R-ALL-141342", "reactome_id": "R-ALL-141342", "label": "SPN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:45725", "formula": "C10H30N4", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-141324", "reactome_id": "R-ALL-141324", "label": "SPM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57834", "formula": "C7H22N3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141328", "reactome_id": "R-ALL-141328", "label": "3APAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58374", "formula": "C3H8NO", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "spermine oxidase activity of SMOX-3 [cytosol]", "activity_id": "5603125", "ec_numbers": [ "1.5.3.16" ], "accessions": [ "Q9NWM0" ] } ], "citations": [ { "id": "PMID:12141946", "title": "Identification and characterization of a novel flavin-containing spermine oxidase of mammalian cell origin", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12141946/" }, { "id": "PMID:12727196", "title": "Properties of purified recombinant human polyamine oxidase, PAOh1/SMO", "journal": "Biochem Biophys Res Commun", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12727196/" } ], "x": 7461.333333333333, "y": 2806.8137254901962, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-141348", "label": "PAO:FAD oxidises NASPM to PTCN", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:25813" ], "source_url": "https://reactome.org/content/detail/R-HSA-141348", "summary": "PAO:FAD oxidises NASPM to PTCN is a Reactome release 97 curated reaction event. The source record connects NASPM, H2O, O2 to PTCN, H2O2, 3AAPNAL in peroxisomal matrix and cross-references RHEA:25813.", "equation": "NASPM + H2O + O2 → PTCN + H2O2 + 3AAPNAL", "inputs": [ { "node_id": "R-ALL-141349", "reactome_id": "R-ALL-141349", "label": "NASPM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58324", "formula": "C9H23N3O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-141350", "reactome_id": "R-ALL-141350", "label": "PTCN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:326268", "formula": "C4H14N2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141327", "reactome_id": "R-ALL-141327", "label": "3AAPNAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30322", "formula": "C5H9NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N(1)-acetylpolyamine oxidase activity of PAOX:FAD [peroxisomal matrix]", "activity_id": "141347", "ec_numbers": [ "1.5.3.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:12477380", "title": "Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12477380/" } ], "x": 7740, "y": 2806.8137254901962, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-141351", "label": "PAOX:FAD oxidises NASPN to SPM", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:25801" ], "source_url": "https://reactome.org/content/detail/R-HSA-141351", "summary": "PAOX:FAD oxidises NASPN to SPM is a Reactome release 97 curated reaction event. The source record connects NASPN, H2O, O2 to SPM, H2O2, 3AAPNAL in peroxisomal matrix and cross-references RHEA:25801.", "equation": "NASPN + H2O + O2 → SPM + H2O2 + 3AAPNAL", "inputs": [ { "node_id": "R-ALL-141323", "reactome_id": "R-ALL-141323", "label": "NASPN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58101", "formula": "C12H31N4O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-141324", "reactome_id": "R-ALL-141324", "label": "SPM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57834", "formula": "C7H22N3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141327", "reactome_id": "R-ALL-141327", "label": "3AAPNAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30322", "formula": "C5H9NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N(1)-acetylpolyamine oxidase activity of PAOX:FAD [peroxisomal matrix]", "activity_id": "141347", "ec_numbers": [ "1.5.3.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:12477380", "title": "Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12477380/" } ], "x": 8018.666666666667, "y": 2806.8137254901962, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-143468", "label": "MEOS oxidizes ethanol to acetaldehyde", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-143468", "summary": "MEOS oxidizes ethanol to acetaldehyde is a Reactome release 97 curated reaction event. The source record connects EtOH, NADPH, H+ to CH3CHO, H2O, NADP+ in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "EtOH + NADPH + H+ + O2 → CH3CHO + 2 H2O + NADP+", "inputs": [ { "node_id": "R-ALL-113748", "reactome_id": "R-ALL-113748", "label": "EtOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16236", "formula": "C2H6O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113745", "reactome_id": "R-ALL-113745", "label": "CH3CHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2E1 based) [endoplasmic reticulum membrane]", "activity_id": "76353", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9028626", "title": "Ethanol metabolism, cirrhosis and alcoholism", "journal": "Clin Chim Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9028626/" }, { "id": "PMID:9884161", "title": "Respective roles of human cytochrome P-4502E1, 1A2, and 3A4 in the hepatic microsomal ethanol oxidizing system", "journal": "Alcohol Clin Exp Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9884161/" }, { "id": "PMID:8627510", "title": "Microsomal ethanol oxidizing system activity by human hepatic cytochrome P450s", "journal": "J Pharmacol Exp Ther", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8627510/" } ], "x": 7461.333333333333, "y": 2951.735294117647, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1449687", "label": "Corticosteroids bind to CBG in blood", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1449687", "summary": "Corticosteroids bind to CBG in blood is a Reactome release 97 curated reaction event. The source record connects SERPINA6, Corticosteroids to CBG:Corticosteroids in extracellular region.", "equation": "SERPINA6 + Corticosteroids → CBG:Corticosteroids", "inputs": [ { "node_id": "R-HSA-1449658", "reactome_id": "R-HSA-1449658", "label": "SERPINA6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-1449707", "reactome_id": "R-ALL-1449707", "label": "Corticosteroids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1449677", "reactome_id": "R-HSA-1449677", "label": "CBG:Corticosteroids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of SERPINA6 [extracellular region]", "activity_id": "9913423", "ec_numbers": null, "accessions": [ "P08185" ] } ], "citations": [ { "id": "PMID:3299377", "title": "Primary structure of human corticosteroid binding globulin, deduced from hepatic and pulmonary cDNAs, exhibits homology with serine protease inhibitors", "journal": "Proc Natl Acad Sci U S A", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3299377/" }, { "id": "PMID:7195404", "title": "Transport of steroid hormones: binding of 21 endogenous steroids to both testosterone-binding globulin and corticosteroid-binding globulin in human plasma", "journal": "J Clin Endocrinol Metab", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/7195404/" } ], "x": 5008.666666666667, "y": 1754.0227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1474146", "label": "GCH1 reduces GTP to dihydroneopterin triphosphate", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17474" ], "source_url": "https://reactome.org/content/detail/R-HSA-1474146", "summary": "GCH1 reduces GTP to dihydroneopterin triphosphate is a Reactome release 97 curated reaction event. The source record connects GTP, H2O to DHNTP, HCOOH in cytosol and cross-references RHEA:17474.", "equation": "GTP + H2O → DHNTP + HCOOH", "inputs": [ { "node_id": "R-ALL-29438", "reactome_id": "R-ALL-29438", "label": "GTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37565", "formula": "C10H12N5O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1474124", "reactome_id": "R-ALL-1474124", "label": "DHNTP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58462", "formula": "C9H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "GTP cyclohydrolase I activity of GCH1 decamer [cytosol]", "activity_id": "1474128", "ec_numbers": [ "3.5.4.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:8068008", "title": "Human GTP cyclohydrolase I: only one out of three cDNA isoforms gives rise to the active enzyme", "journal": "Biochem J", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8068008/" }, { "id": "PMID:7663943", "title": "Atomic structure of GTP cyclohydrolase I", "journal": "Structure", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7663943/" } ], "x": 4730, "y": 3395.4583333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1474158", "label": "GCHFR binds to GCH1 and negatively regulates its activity", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1474158", "summary": "GCHFR binds to GCH1 and negatively regulates its activity is a Reactome release 97 curated reaction event. The source record connects GCH1 decamer, GCHFR pentamer to 2GCHFR:GCH1 in cytosol.", "equation": "GCH1 decamer + 2 GCHFR pentamer → 2GCHFR:GCH1", "inputs": [ { "node_id": "R-HSA-1474144", "reactome_id": "R-HSA-1474144", "label": "GCH1 decamer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1474155", "reactome_id": "R-HSA-1474155", "label": "GCHFR pentamer", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1474149", "reactome_id": "R-HSA-1474149", "label": "2GCHFR:GCH1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "enzyme inhibitor activity of GCHFR pentamer [cytosol]", "activity_id": "9959889", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:16696853", "title": "A yeast 2-hybrid analysis of human GTP cyclohydrolase I protein interactions", "journal": "J Neurochem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16696853/" }, { "id": "PMID:8502995", "title": "Feedback regulation mechanisms for the control of GTP cyclohydrolase I activity", "journal": "Science", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8502995/" }, { "id": "PMID:16778797", "title": "GTP cyclohydrolase feedback regulatory protein controls cofactor 6-tetrahydrobiopterin synthesis in the cytosol and in the nucleus of epidermal keratinocytes and melanocytes", "journal": "J Invest Dermatol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16778797/" } ], "x": 4730, "y": 3622.375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1474184", "label": "DHNTP is dephosphorylated by PTPS to PTHP", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1474184", "summary": "DHNTP is dephosphorylated by PTPS to PTHP is a Reactome release 97 curated reaction event. The source record connects DHNTP to PTHP, PPP in cytosol.", "equation": "DHNTP → PTHP + PPP", "inputs": [ { "node_id": "R-ALL-1474124", "reactome_id": "R-ALL-1474124", "label": "DHNTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58462", "formula": "C9H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1474179", "reactome_id": "R-ALL-1474179", "label": "PTHP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17804", "formula": "C9H11N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1475054", "reactome_id": "R-ALL-1475054", "label": "PPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15266", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "6-pyruvoyltetrahydropterin synthase activity of p-PTPS hexamer [cytosol]", "activity_id": "1475060", "ec_numbers": [ "4.2.3.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:3536512", "title": "Biosynthesis of tetrahydrobiopterin. Purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver", "journal": "Eur J Biochem", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3536512/" }, { "id": "PMID:7563095", "title": "6-Pyruvoyl tetrahydropterin synthase, an enzyme with a novel type of active site involving both zinc binding and an intersubunit catalytic triad motif; site-directed mutagenesis of the proposed active center, characterization of the metal binding site and modelling of substrate binding", "journal": "J Mol Biol", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7563095/" }, { "id": "PMID:8307017", "title": "Expression and characterization of recombinant human and rat liver 6-pyruvoyl tetrahydropterin synthase. Modified cysteine residues inhibit the enzyme activity", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8307017/" }, { "id": "PMID:8137809", "title": "Three-dimensional structure of 6-pyruvoyl tetrahydropterin synthase, an enzyme involved in tetrahydrobiopterin biosynthesis", "journal": "EMBO J", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8137809/" }, { "id": "PMID:10531334", "title": "Serine 19 of human 6-pyruvoyltetrahydropterin synthase is phosphorylated by cGMP protein kinase II", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10531334/" } ], "x": 4451.333333333333, "y": 3492.7083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1475017", "label": "Carbonic anhydrase dehydrates bicarbonate (plasma membrane)", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "carbon-dioxide" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:10749" ], "source_url": "https://reactome.org/content/detail/R-HSA-1475017", "summary": "Carbonic anhydrase dehydrates bicarbonate (plasma membrane) is a Reactome release 97 curated reaction event. The source record connects H+, HCO3- to CO2, H2O in plasma membrane · extracellular region and cross-references RHEA:10749.", "equation": "H+ + HCO3- → CO2 + H2O", "inputs": [ { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-425425", "reactome_id": "R-ALL-425425", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1237009", "reactome_id": "R-ALL-1237009", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbonate dehydratase activity of CA4,9,14,12:Zn2+ [plasma membrane]", "activity_id": "1475013", "ec_numbers": [ "4.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2111324", "title": "Carbonic anhydrase IV from human lung. Purification, characterization, and comparison with membrane carbonic anhydrase from human kidney", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2111324/" }, { "id": "PMID:7625839", "title": "Carbonic anhydrase IV: role of removal of C-terminal domain in glycosylphosphatidylinositol anchoring and realization of enzyme activity", "journal": "Arch Biochem Biophys", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7625839/" }, { "id": "PMID:15501038", "title": "Carbonic anhydrase inhibitors: inhibition of the membrane-bound human isozyme IV with anions", "journal": "Bioorg Med Chem Lett", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15501038/" }, { "id": "PMID:9054574", "title": "Catalysis and inhibition of human carbonic anhydrase IV", "journal": "Biochemistry", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9054574/" }, { "id": "PMID:9336012", "title": "Structure and mechanism of carbonic anhydrase", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9336012/" }, { "id": "PMID:18703501", "title": "Biochemical characterization of CA IX, one of the most active carbonic anhydrase isozymes", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18703501/" } ], "x": 7078.666666666667, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1475022", "label": "Carbonic anhydrase dehydrates bicarbonate (cytosol)", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "carbon-dioxide" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10749" ], "source_url": "https://reactome.org/content/detail/R-HSA-1475022", "summary": "Carbonic anhydrase dehydrates bicarbonate (cytosol) is a Reactome release 97 curated reaction event. The source record connects H+, HCO3- to CO2, H2O in cytosol and cross-references RHEA:10749.", "equation": "H+ + HCO3- → CO2 + H2O", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111627", "reactome_id": "R-ALL-111627", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbonate dehydratase activity of CA1,2,3,7,13:Zinc [cytosol]", "activity_id": "1475014", "ec_numbers": [ "4.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:4994926", "title": "The carbon dioxide hydration activity of carbonic anhydrase. I. Stop-flow kinetic studies on the native human isoenzymes B and C", "journal": "J Biol Chem", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/4994926/" }, { "id": "PMID:6819139", "title": "A 13C nuclear magnetic resonance study of CO2/HCO-3 exchange catalyzed by human carbonic anhydrase I", "journal": "Eur J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6819139/" }, { "id": "PMID:1554744", "title": "Buffer dependence of CO2 hydration catalyzed by human carbonic anhydrase I", "journal": "Biochim Biophys Acta", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1554744/" }, { "id": "PMID:9336012", "title": "Structure and mechanism of carbonic anhydrase", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9336012/" }, { "id": "PMID:6433979", "title": "Anion inhibition of CO2 hydration catalyzed by human carbonic anhydrase II. Mechanistic implications", "journal": "Biochim Biophys Acta", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6433979/" }, { "id": "PMID:6407977", "title": "A chemical and enzymological comparison of the common major human erythrocyte carbonic anhydrase II, its minor component, and a new genetic variant, CA II Melbourne (237 Pro leads to His)", "journal": "Hum Genet", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6407977/" } ], "x": 6521.333333333333, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1475025", "label": "Carbonic anhydrase hydrates carbon dioxide (plasma membrane)", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "carbon-dioxide" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:10750" ], "source_url": "https://reactome.org/content/detail/R-HSA-1475025", "summary": "Carbonic anhydrase hydrates carbon dioxide (plasma membrane) is a Reactome release 97 curated reaction event. The source record connects CO2, H2O to H+, HCO3- in plasma membrane · extracellular region and cross-references RHEA:10750.", "equation": "CO2 + H2O → H+ + HCO3-", "inputs": [ { "node_id": "R-ALL-1237009", "reactome_id": "R-ALL-1237009", "label": "CO2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-425425", "reactome_id": "R-ALL-425425", "label": "HCO3-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbonate dehydratase activity of CA4,9,14,12:Zn2+ [plasma membrane]", "activity_id": "1475013", "ec_numbers": [ "4.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2111324", "title": "Carbonic anhydrase IV from human lung. Purification, characterization, and comparison with membrane carbonic anhydrase from human kidney", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2111324/" }, { "id": "PMID:7625839", "title": "Carbonic anhydrase IV: role of removal of C-terminal domain in glycosylphosphatidylinositol anchoring and realization of enzyme activity", "journal": "Arch Biochem Biophys", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7625839/" }, { "id": "PMID:15501038", "title": "Carbonic anhydrase inhibitors: inhibition of the membrane-bound human isozyme IV with anions", "journal": "Bioorg Med Chem Lett", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15501038/" }, { "id": "PMID:9054574", "title": "Catalysis and inhibition of human carbonic anhydrase IV", "journal": "Biochemistry", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9054574/" }, { "id": "PMID:9336012", "title": "Structure and mechanism of carbonic anhydrase", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9336012/" }, { "id": "PMID:18703501", "title": "Biochemical characterization of CA IX, one of the most active carbonic anhydrase isozymes", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18703501/" } ], "x": 6800, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1475026", "label": "Carbonic anhydrase hydrates carbon dioxide (cytosol)", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "carbon-dioxide" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10750" ], "source_url": "https://reactome.org/content/detail/R-HSA-1475026", "summary": "Carbonic anhydrase hydrates carbon dioxide (cytosol) is a Reactome release 97 curated reaction event. The source record connects CO2, H2O to H+, HCO3- in cytosol and cross-references RHEA:10750.", "equation": "CO2 + H2O → H+ + HCO3-", "inputs": [ { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111627", "reactome_id": "R-ALL-111627", "label": "HCO3-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbonate dehydratase activity of CA1,2,3,7,13:Zinc [cytosol]", "activity_id": "1475014", "ec_numbers": [ "4.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:4994926", "title": "The carbon dioxide hydration activity of carbonic anhydrase. I. Stop-flow kinetic studies on the native human isoenzymes B and C", "journal": "J Biol Chem", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/4994926/" }, { "id": "PMID:6819139", "title": "A 13C nuclear magnetic resonance study of CO2/HCO-3 exchange catalyzed by human carbonic anhydrase I", "journal": "Eur J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6819139/" }, { "id": "PMID:1554744", "title": "Buffer dependence of CO2 hydration catalyzed by human carbonic anhydrase I", "journal": "Biochim Biophys Acta", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1554744/" }, { "id": "PMID:9336012", "title": "Structure and mechanism of carbonic anhydrase", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9336012/" }, { "id": "PMID:6433979", "title": "Anion inhibition of CO2 hydration catalyzed by human carbonic anhydrase II. Mechanistic implications", "journal": "Biochim Biophys Acta", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6433979/" }, { "id": "PMID:6407977", "title": "A chemical and enzymological comparison of the common major human erythrocyte carbonic anhydrase II, its minor component, and a new genetic variant, CA II Melbourne (237 Pro leads to His)", "journal": "Hum Genet", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6407977/" } ], "x": 7078.666666666667, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1475028", "label": "Carbonic anhydrase dehydrates bicarbonate (mitochondria)", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "carbon-dioxide" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:10749" ], "source_url": "https://reactome.org/content/detail/R-HSA-1475028", "summary": "Carbonic anhydrase dehydrates bicarbonate (mitochondria) is a Reactome release 97 curated reaction event. The source record connects H+, HCO3- to CO2, H2O in mitochondrial matrix and cross-references RHEA:10749.", "equation": "H+ + HCO3- → CO2 + H2O", "inputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29896", "reactome_id": "R-ALL-29896", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbonate dehydratase activity of CA5A,B:Zinc [mitochondrial matrix]", "activity_id": "1475031", "ec_numbers": [ "4.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16302824", "title": "Carbonic anhydrase inhibitors. The mitochondrial isozyme VB as a new target for sulfonamide and sulfamate inhibitors", "journal": "J Med Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16302824/" }, { "id": "PMID:14611844", "title": "Carbonic anhydrase inhibitors: inhibition of human and murine mitochondrial isozymes V with anions", "journal": "Bioorg Med Chem Lett", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14611844/" }, { "id": "PMID:17761422", "title": "Carbonic anhydrase inhibitors: the inhibition profiles of the human mitochondrial isoforms VA and VB with anions are very different", "journal": "Bioorg Med Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17761422/" }, { "id": "PMID:10409679", "title": "Human mitochondrial carbonic anhydrase VB. cDNA cloning, mRNA expression, subcellular localization, and mapping to chromosome x", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10409679/" }, { "id": "PMID:8356065", "title": "Human mitochondrial carbonic anhydrase: cDNA cloning, expression, subcellular localization, and mapping to chromosome 16", "journal": "Proc Natl Acad Sci U S A", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8356065/" }, { "id": "PMID:9336012", "title": "Structure and mechanism of carbonic anhydrase", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9336012/" } ], "x": 6521.333333333333, "y": 1066.1666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1475032", "label": "Carbonic anhydrase hydrates carbon dioxide (mitochondria)", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "carbon-dioxide" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:10750" ], "source_url": "https://reactome.org/content/detail/R-HSA-1475032", "summary": "Carbonic anhydrase hydrates carbon dioxide (mitochondria) is a Reactome release 97 curated reaction event. The source record connects CO2, H2O to H+, HCO3- in mitochondrial matrix and cross-references RHEA:10750.", "equation": "CO2 + H2O → H+ + HCO3-", "inputs": [ { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29896", "reactome_id": "R-ALL-29896", "label": "HCO3-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbonate dehydratase activity of CA5A,B:Zinc [mitochondrial matrix]", "activity_id": "1475031", "ec_numbers": [ "4.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16302824", "title": "Carbonic anhydrase inhibitors. The mitochondrial isozyme VB as a new target for sulfonamide and sulfamate inhibitors", "journal": "J Med Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16302824/" }, { "id": "PMID:14611844", "title": "Carbonic anhydrase inhibitors: inhibition of human and murine mitochondrial isozymes V with anions", "journal": "Bioorg Med Chem Lett", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14611844/" }, { "id": "PMID:17761422", "title": "Carbonic anhydrase inhibitors: the inhibition profiles of the human mitochondrial isoforms VA and VB with anions are very different", "journal": "Bioorg Med Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17761422/" }, { "id": "PMID:10409679", "title": "Human mitochondrial carbonic anhydrase VB. cDNA cloning, mRNA expression, subcellular localization, and mapping to chromosome x", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10409679/" }, { "id": "PMID:8356065", "title": "Human mitochondrial carbonic anhydrase: cDNA cloning, expression, subcellular localization, and mapping to chromosome 16", "journal": "Proc Natl Acad Sci U S A", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8356065/" }, { "id": "PMID:9336012", "title": "Structure and mechanism of carbonic anhydrase", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9336012/" } ], "x": 6800, "y": 1066.1666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1475414", "label": "PTHP is reduced to BH4 by sepiapterin reductase (SPR)", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1475414", "summary": "PTHP is reduced to BH4 by sepiapterin reductase (SPR) is a Reactome release 97 curated reaction event. The source record connects PTHP, NADPH to BH4, NADP+ in cytosol.", "equation": "PTHP + 2 NADPH → BH4 + 2 NADP+", "inputs": [ { "node_id": "R-ALL-1474179", "reactome_id": "R-ALL-1474179", "label": "PTHP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17804", "formula": "C9H11N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sepiapterin reductase (NADP+) activity of p-SPR dimer [cytosol]", "activity_id": "1497839", "ec_numbers": [ "1.1.1.153" ], "accessions": [] } ], "citations": [ { "id": "PMID:1883349", "title": "Cloning and sequencing of cDNA encoding human sepiapterin reductase--an enzyme involved in tetrahydrobiopterin biosynthesis", "journal": "Biochem Biophys Res Commun", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1883349/" } ], "x": 4730, "y": 3460.2916666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1475422", "label": "PTPS is phosphorylated by cGMP-dependant protein kinase II", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1475422", "summary": "PTPS is phosphorylated by cGMP-dependant protein kinase II is a Reactome release 97 curated reaction event. The source record connects ATP, PTPS hexamer to p-PTPS hexamer, ADP in cytosol.", "equation": "6 ATP + PTPS hexamer → p-PTPS hexamer + 6 ADP", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 6, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-1497879", "reactome_id": "R-HSA-1497879", "label": "PTPS hexamer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1475058", "reactome_id": "R-HSA-1475058", "label": "p-PTPS hexamer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 6, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cGMP-dependent protein kinase activity of PRKG2 [cytosol]", "activity_id": "1475413", "ec_numbers": [ "2.7.11.12" ], "accessions": [ "Q13237" ] } ], "citations": [ { "id": "PMID:10531334", "title": "Serine 19 of human 6-pyruvoyltetrahydropterin synthase is phosphorylated by cGMP protein kinase II", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10531334/" } ], "x": 4730, "y": 3492.7083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482533", "label": "2-acyl LPC is acylated to PC by LPCAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:10332" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482533", "summary": "2-acyl LPC is acylated to PC by LPCAT is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPC, acyl-CoA to PC, CoA-SH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:10332.", "equation": "2-acyl LPC + acyl-CoA → PC + CoA-SH", "inputs": [ { "node_id": "R-ALL-975614", "reactome_id": "R-ALL-975614", "label": "2-acyl LPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16728", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-acylglycerol-3-phosphate O-acyltransferase activity of LPCAT [endoplasmic reticulum membrane]", "activity_id": "1524043", "ec_numbers": [ "2.3.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:18458083", "title": "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18458083/" }, { "id": "PMID:16243729", "title": "Cloning and characterization a novel human 1-acyl-sn-glycerol-3-phosphate acyltransferase gene AGPAT7", "journal": "DNA Seq", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16243729/" }, { "id": "PMID:18772128", "title": "Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18772128/" }, { "id": "PMID:16704971", "title": "Cloning and characterization of mouse lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 (LPCAT1). Expression in alveolar type II cells and possible involvement in surfactant production", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16704971/" }, { "id": "PMID:16864775", "title": "Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16864775/" }, { "id": "PMID:17182612", "title": "A single enzyme catalyzes both platelet-activating factor production and membrane biogenesis of inflammatory cells. Cloning and characterization of acetyl-CoA:LYSO-PAF acetyltransferase", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17182612/" } ], "x": 2938.666666666667, "y": 1540.9931506849316, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482539", "label": "1-acyl LPG is acylated to PG by LPGAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482539", "summary": "1-acyl LPG is acylated to PG by LPGAT is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPG, acyl-CoA to PG, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "1-acyl LPG + acyl-CoA → PG + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498740", "reactome_id": "R-ALL-1498740", "label": "1-acyl LPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62747", "formula": "C7H14O9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1483256", "reactome_id": "R-ALL-1483256", "label": "PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of LPGAT [endoplasmic reticulum membrane]", "activity_id": "1524031", "ec_numbers": [ "2.3.1.51" ], "accessions": [] } ], "citations": [ { "id": "PMID:18458083", "title": "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18458083/" }, { "id": "PMID:16243729", "title": "Cloning and characterization a novel human 1-acyl-sn-glycerol-3-phosphate acyltransferase gene AGPAT7", "journal": "DNA Seq", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16243729/" }, { "id": "PMID:15485873", "title": "Identification and characterization of a gene encoding human LPGAT1, an endoplasmic reticulum-associated lysophosphatidylglycerol acyltransferase", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15485873/" }, { "id": "PMID:16704971", "title": "Cloning and characterization of mouse lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 (LPCAT1). Expression in alveolar type II cells and possible involvement in surfactant production", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16704971/" }, { "id": "PMID:16864775", "title": "Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16864775/" } ], "x": 2381.3333333333335, "y": 1674.2123287671234, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482543", "label": "2-MAG is hydrolyzed to fatty acid and glycerol by MGLL", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:44688" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482543", "summary": "2-MAG is hydrolyzed to fatty acid and glycerol by MGLL is a Reactome release 97 curated reaction event. The source record connects 2-MAG, H2O to Glycerol, fatty acid in endoplasmic reticulum membrane · cytosol and cross-references RHEA:44688.", "equation": "2-MAG + H2O → Glycerol + fatty acid", "inputs": [ { "node_id": "R-ALL-1498728", "reactome_id": "R-ALL-1498728", "label": "2-MAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17389", "formula": "C4H7O4R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76116", "reactome_id": "R-ALL-76116", "label": "Glycerol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17754", "formula": "C3H8O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500593", "reactome_id": "R-ALL-1500593", "label": "fatty acid", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35366", "formula": "CHO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monoacylglycerol lipase activity of MGLL dimer [endoplasmic reticulum membrane]", "activity_id": "1500582", "ec_numbers": [ "3.1.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:18452279", "title": "Full mass spectrometric characterization of human monoacylglycerol lipase generated by large-scale expression and single-step purification", "journal": "J Proteome Res", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18452279/" }, { "id": "PMID:15272052", "title": "RNA interference suggests a primary role for monoacylglycerol lipase in the degradation of the endocannabinoid 2-arachidonoylglycerol", "journal": "Mol Pharmacol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15272052/" }, { "id": "PMID:19957260", "title": "Crystal structure of the human monoacylglycerol lipase, a key actor in endocannabinoid signaling", "journal": "Chembiochem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19957260/" }, { "id": "PMID:19962385", "title": "Structural basis for human monoglyceride lipase inhibition", "journal": "J Mol Biol", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19962385/" } ], "x": 2381.3333333333335, "y": 1546.3219178082193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482545", "label": "2-acyl LPE is hydrolyzed to GPETA by PLA2G4C", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482545", "summary": "2-acyl LPE is hydrolyzed to GPETA by PLA2G4C is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPE, H2O to GPETA, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "2-acyl LPE + H2O → GPETA + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1498729", "reactome_id": "R-ALL-1498729", "label": "2-acyl LPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28936", "formula": "C6H13NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1524023", "reactome_id": "R-ALL-1524023", "label": "GPETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16929", "formula": "C5H14NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine lysophospholipase A1 activity of PLA2G4C [endoplasmic reticulum membrane]", "activity_id": "1524030", "ec_numbers": [ "3.1.1.5" ], "accessions": [ "Q9UP65" ] } ], "citations": [ { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" }, { "id": "PMID:15944408", "title": "Roles of C-terminal processing, and involvement in transacylation reaction of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "J Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15944408/" } ], "x": 2660, "y": 1674.2123287671234, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482546", "label": "2-acyl LPG is acylated to PG by CRLS1 (IM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482546", "summary": "2-acyl LPG is acylated to PG by CRLS1 (IM) is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPG, acyl-CoA to PG, CoA-SH in mitochondrial inner membrane · mitochondrial matrix.", "equation": "2-acyl LPG + acyl-CoA → PG + CoA-SH", "inputs": [ { "node_id": "R-ALL-1524047", "reactome_id": "R-ALL-1524047", "label": "2-acyl LPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27923", "formula": "C7H14O9PR", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524038", "reactome_id": "R-ALL-1524038", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524042", "reactome_id": "R-ALL-1524042", "label": "PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-acylglycerol-3-phosphate O-acyltransferase activity of CRLS1 [mitochondrial inner membrane]", "activity_id": "1524051", "ec_numbers": [ "2.3.1.52" ], "accessions": [ "Q9UJA2" ] } ], "citations": [ { "id": "PMID:20025994", "title": "A novel function of the human CLS1 in phosphatidylglycerol synthesis and remodeling", "journal": "Biochim Biophys Acta", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20025994/" } ], "x": 2938.666666666667, "y": 1674.2123287671234, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482547", "label": "1-acyl LPC is acylated to PC by LPCAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:12937" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482547", "summary": "1-acyl LPC is acylated to PC by LPCAT is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPC, acyl-CoA to PC, CoA-SH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:12937.", "equation": "1-acyl LPC + acyl-CoA → PC + CoA-SH", "inputs": [ { "node_id": "R-ALL-1524028", "reactome_id": "R-ALL-1524028", "label": "1-acyl LPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of LPCAT [endoplasmic reticulum membrane]", "activity_id": "1524024", "ec_numbers": [ "2.3.1.51" ], "accessions": [] } ], "citations": [ { "id": "PMID:18458083", "title": "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18458083/" }, { "id": "PMID:16243729", "title": "Cloning and characterization a novel human 1-acyl-sn-glycerol-3-phosphate acyltransferase gene AGPAT7", "journal": "DNA Seq", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16243729/" }, { "id": "PMID:18772128", "title": "Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18772128/" }, { "id": "PMID:16704971", "title": "Cloning and characterization of mouse lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 (LPCAT1). Expression in alveolar type II cells and possible involvement in surfactant production", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16704971/" }, { "id": "PMID:16864775", "title": "Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16864775/" }, { "id": "PMID:17182612", "title": "A single enzyme catalyzes both platelet-activating factor production and membrane biogenesis of inflammatory cells. Cloning and characterization of acetyl-CoA:LYSO-PAF acetyltransferase", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17182612/" } ], "x": 2660, "y": 1546.3219178082193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482548", "label": "1-acyl LPA is acylated to PA by AGPAT5 (OM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial outer membrane · cytosol", "compartment_ids": [ "GO:0005741", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482548", "summary": "1-acyl LPA is acylated to PA by AGPAT5 (OM) is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPA, acyl-CoA to PA, CoA-SH in mitochondrial outer membrane · cytosol.", "equation": "1-acyl LPA + acyl-CoA → PA + CoA-SH", "inputs": [ { "node_id": "R-ALL-1500580", "reactome_id": "R-ALL-1500580", "label": "1-acyl LPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500590", "reactome_id": "R-ALL-1500590", "label": "PA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of AGPAT5 [mitochondrial outer membrane]", "activity_id": "1500599", "ec_numbers": [ "2.3.1.51" ], "accessions": [ "Q9NUQ2" ] } ], "citations": [ { "id": "PMID:21173190", "title": "Enzymatic activities of the human AGPAT isoform 3 and isoform 5: localization of AGPAT5 to mitochondria", "journal": "J Lipid Res", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21173190/" } ], "x": 2381.3333333333335, "y": 1679.541095890411, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482571", "label": "1-acyl LPE is hydrolyzed to GPETA by PLA2G4C", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482571", "summary": "1-acyl LPE is hydrolyzed to GPETA by PLA2G4C is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPE, H2O to GPETA, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "1-acyl LPE + H2O → GPETA + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1498738", "reactome_id": "R-ALL-1498738", "label": "1-acyl LPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29017", "formula": "C6H13NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1524023", "reactome_id": "R-ALL-1524023", "label": "GPETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16929", "formula": "C5H14NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine lysophospholipase A1 activity of PLA2G4C [endoplasmic reticulum membrane]", "activity_id": "1524030", "ec_numbers": [ "3.1.1.5" ], "accessions": [ "Q9UP65" ] } ], "citations": [ { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" }, { "id": "PMID:15944408", "title": "Roles of C-terminal processing, and involvement in transacylation reaction of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "J Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15944408/" } ], "x": 2660, "y": 1679.541095890411, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482598", "label": "1-acyl LPI is acylated to PI by MBOAT7", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:33195" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482598", "summary": "1-acyl LPI is acylated to PI by MBOAT7 is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPI, acyl-CoA to PI, CoA-SH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:33195.", "equation": "1-acyl LPI + acyl-CoA → PI + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498733", "reactome_id": "R-ALL-1498733", "label": "1-acyl LPI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28914", "formula": "C10H18O12PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of MBOAT7 [endoplasmic reticulum membrane]", "activity_id": "1524032", "ec_numbers": [ "2.3.1.51" ], "accessions": [ "Q96N66" ] } ], "citations": [ { "id": "PMID:18772128", "title": "Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18772128/" }, { "id": "PMID:18094042", "title": "Caenorhabditis elegans mboa-7, a member of the MBOAT family, is required for selective incorporation of polyunsaturated fatty acids into phosphatidylinositol", "journal": "Mol Biol Cell", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18094042/" } ], "x": 2938.666666666667, "y": 1546.3219178082193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482604", "label": "PA is hydrolyzed to 1-acyl LPA by PLA2[1] (OM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial outer membrane · cytosol", "compartment_ids": [ "GO:0005741", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482604", "summary": "PA is hydrolyzed to 1-acyl LPA by PLA2[1] (OM) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PA, H2O to 1-acyl LPA, LCFA(-) in mitochondrial outer membrane · cytosol.", "equation": "PA + H2O → 1-acyl LPA + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500590", "reactome_id": "R-ALL-1500590", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1500580", "reactome_id": "R-ALL-1500580", "label": "1-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524103", "reactome_id": "R-ALL-1524103", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(1) [cytosol]", "activity_id": "1500640", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2938.666666666667, "y": 1679.541095890411, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482612", "label": "2-acyl LPC is hydrolyzed to GPCho by PLA2[8]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482612", "summary": "2-acyl LPC is hydrolyzed to GPCho by PLA2[8] is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPC, H2O to GPCho, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "2-acyl LPC + H2O → GPCho + LCFA(-)", "inputs": [ { "node_id": "R-ALL-975614", "reactome_id": "R-ALL-975614", "label": "2-acyl LPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16728", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498750", "reactome_id": "R-ALL-1498750", "label": "GPCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16870", "formula": "C8H20NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine lysophospholipase A1 activity of PLA2(8) [cytosol]", "activity_id": "1524022", "ec_numbers": [ "3.1.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:19019078", "title": "The identification of a phospholipase B precursor in human neutrophils", "journal": "FEBS J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19019078/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:8083230", "title": "Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8083230/" }, { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" }, { "id": "PMID:15944408", "title": "Roles of C-terminal processing, and involvement in transacylation reaction of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "J Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15944408/" } ], "x": 2381.3333333333335, "y": 1684.8698630136987, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482626", "label": "2-acyl LPI is acylated to PI by MBOAT7", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:35271" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482626", "summary": "2-acyl LPI is acylated to PI by MBOAT7 is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPI, acyl-CoA to PI, CoA-SH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:35271.", "equation": "2-acyl LPI + acyl-CoA → PI + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498737", "reactome_id": "R-ALL-1498737", "label": "2-acyl LPI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62746", "formula": "C10H18O12PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-acylglycerol-3-phosphate O-acyltransferase activity of MBOAT7 [endoplasmic reticulum membrane]", "activity_id": "1524055", "ec_numbers": [ "2.3.1.52" ], "accessions": [ "Q96N66" ] } ], "citations": [ { "id": "PMID:18772128", "title": "Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18772128/" }, { "id": "PMID:18094042", "title": "Caenorhabditis elegans mboa-7, a member of the MBOAT family, is required for selective incorporation of polyunsaturated fatty acids into phosphatidylinositol", "journal": "Mol Biol Cell", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18094042/" } ], "x": 2381.3333333333335, "y": 1551.650684931507, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482629", "label": "2-acyl LPC is hydrolyzed to GPCho by PLA2G4C", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482629", "summary": "2-acyl LPC is hydrolyzed to GPCho by PLA2G4C is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPC, H2O to GPCho, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "2-acyl LPC + H2O → GPCho + LCFA(-)", "inputs": [ { "node_id": "R-ALL-975614", "reactome_id": "R-ALL-975614", "label": "2-acyl LPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16728", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498750", "reactome_id": "R-ALL-1498750", "label": "GPCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16870", "formula": "C8H20NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine lysophospholipase A1 activity of PLA2G4C [endoplasmic reticulum membrane]", "activity_id": "1524030", "ec_numbers": [ "3.1.1.5" ], "accessions": [ "Q9UP65" ] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" }, { "id": "PMID:15944408", "title": "Roles of C-terminal processing, and involvement in transacylation reaction of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "J Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15944408/" } ], "x": 2660, "y": 1684.8698630136987, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482635", "label": "2-acyl LPG is acylated to PG by LPGAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482635", "summary": "2-acyl LPG is acylated to PG by LPGAT is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPG, acyl-CoA to PG, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "2-acyl LPG + acyl-CoA → PG + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498730", "reactome_id": "R-ALL-1498730", "label": "2-acyl LPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27923", "formula": "C7H14O9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1483256", "reactome_id": "R-ALL-1483256", "label": "PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-acylglycerol-3-phosphate O-acyltransferase activity of LPGAT [endoplasmic reticulum membrane]", "activity_id": "1524046", "ec_numbers": [ "2.3.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:18458083", "title": "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18458083/" }, { "id": "PMID:16243729", "title": "Cloning and characterization a novel human 1-acyl-sn-glycerol-3-phosphate acyltransferase gene AGPAT7", "journal": "DNA Seq", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16243729/" }, { "id": "PMID:15485873", "title": "Identification and characterization of a gene encoding human LPGAT1, an endoplasmic reticulum-associated lysophosphatidylglycerol acyltransferase", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15485873/" }, { "id": "PMID:16704971", "title": "Cloning and characterization of mouse lung-type acyl-CoA:lysophosphatidylcholine acyltransferase 1 (LPCAT1). Expression in alveolar type II cells and possible involvement in surfactant production", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16704971/" }, { "id": "PMID:16864775", "title": "Identification and characterization of a lysophosphatidylcholine acyltransferase in alveolar type II cells", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16864775/" } ], "x": 2938.666666666667, "y": 1684.8698630136987, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482636", "label": "1-acyl LPS is acylated to PS by LPSAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:33191" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482636", "summary": "1-acyl LPS is acylated to PS by LPSAT is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPS, acyl-CoA to PS, CoA-SH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:33191.", "equation": "1-acyl LPS + acyl-CoA → PS + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498724", "reactome_id": "R-ALL-1498724", "label": "1-acyl LPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:52603", "formula": "C7H13NO9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of LPSAT [endoplasmic reticulum membrane]", "activity_id": "1524041", "ec_numbers": [ "2.3.1.51" ], "accessions": [] } ], "citations": [ { "id": "PMID:18287005", "title": "Discovery of a lysophospholipid acyltransferase family essential for membrane asymmetry and diversity", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18287005/" }, { "id": "PMID:18772128", "title": "Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18772128/" }, { "id": "PMID:18458083", "title": "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18458083/" } ], "x": 2660, "y": 1551.650684931507, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482646", "label": "2-acyl LPE is acylated to PE by LPEAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482646", "summary": "2-acyl LPE is acylated to PE by LPEAT is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPE, acyl-CoA to PE, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "2-acyl LPE + acyl-CoA → PE + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498729", "reactome_id": "R-ALL-1498729", "label": "2-acyl LPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28936", "formula": "C6H13NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-acylglycerol-3-phosphate O-acyltransferase activity of LPEAT [endoplasmic reticulum membrane]", "activity_id": "1524045", "ec_numbers": [ "2.3.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:18287005", "title": "Discovery of a lysophospholipid acyltransferase family essential for membrane asymmetry and diversity", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18287005/" }, { "id": "PMID:18772128", "title": "Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18772128/" }, { "id": "PMID:18195019", "title": "Identification and characterization of a major liver lysophosphatidylcholine acyltransferase", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18195019/" }, { "id": "PMID:19351971", "title": "Characterization of human lysophospholipid acyltransferase 3", "journal": "J Lipid Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19351971/" }, { "id": "PMID:18458083", "title": "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18458083/" }, { "id": "PMID:18781350", "title": "Substrate preferences of a lysophosphatidylcholine acyltransferase highlight its role in phospholipid remodeling", "journal": "Lipids", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18781350/" } ], "x": 2381.3333333333335, "y": 1690.1986301369864, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482647", "label": "2-MAG and DAG are transacylated to TAG by PNPLA2/3", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482647", "summary": "2-MAG and DAG are transacylated to TAG by PNPLA2/3 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 2-MAG, DAG to TAG, Glycerol in endoplasmic reticulum membrane · cytosol.", "equation": "2-MAG + DAG → TAG + Glycerol", "inputs": [ { "node_id": "R-ALL-1498728", "reactome_id": "R-ALL-1498728", "label": "2-MAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17389", "formula": "C4H7O4R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500594", "reactome_id": "R-ALL-1500594", "label": "TAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76116", "reactome_id": "R-ALL-76116", "label": "Glycerol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17754", "formula": "C3H8O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acylglycerol O-acyltransferase activity of PNPLA2/3 [endoplasmic reticulum membrane]", "activity_id": "1500577", "ec_numbers": [ "2.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:15364929", "title": "Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15364929/" } ], "x": 2660, "y": 1690.1986301369864, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482654", "label": "2-MAG is transacylated to DAG by PNPLA2/3", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482654", "summary": "2-MAG is transacylated to DAG by PNPLA2/3 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 2-MAG to DAG, Glycerol in endoplasmic reticulum membrane · cytosol.", "equation": "2 2-MAG → DAG + Glycerol", "inputs": [ { "node_id": "R-ALL-1498728", "reactome_id": "R-ALL-1498728", "label": "2-MAG", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:17389", "formula": "C4H7O4R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76116", "reactome_id": "R-ALL-76116", "label": "Glycerol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17754", "formula": "C3H8O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acylglycerol O-acyltransferase activity of PNPLA2/3 [endoplasmic reticulum membrane]", "activity_id": "1500577", "ec_numbers": [ "2.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:15364929", "title": "Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15364929/" } ], "x": 2381.3333333333335, "y": 1850.0616438356165, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482656", "label": "PA is hydrolysed to 1-acyl LPA by PLA2[1]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482656", "summary": "PA is hydrolysed to 1-acyl LPA by PLA2[1] is a Reactome release 97 curated reaction event. The source record connects PA, H2O to 1-acyl LPA, LCFA(-) in cytosol · endoplasmic reticulum membrane.", "equation": "PA + H2O → 1-acyl LPA + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426755", "reactome_id": "R-ALL-426755", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1500604", "reactome_id": "R-ALL-1500604", "label": "1-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(1) [cytosol]", "activity_id": "1500640", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2938.666666666667, "y": 1690.1986301369864, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482667", "label": "1-acyl LPE is acylated to PE by LPEAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482667", "summary": "1-acyl LPE is acylated to PE by LPEAT is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPE, acyl-CoA to PE, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "1-acyl LPE + acyl-CoA → PE + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498738", "reactome_id": "R-ALL-1498738", "label": "1-acyl LPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29017", "formula": "C6H13NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of LPEAT [endoplasmic reticulum membrane]", "activity_id": "1524039", "ec_numbers": [ "2.3.1.51" ], "accessions": [] } ], "citations": [ { "id": "PMID:18287005", "title": "Discovery of a lysophospholipid acyltransferase family essential for membrane asymmetry and diversity", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18287005/" }, { "id": "PMID:18195019", "title": "Identification and characterization of a major liver lysophosphatidylcholine acyltransferase", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18195019/" }, { "id": "PMID:19351971", "title": "Characterization of human lysophospholipid acyltransferase 3", "journal": "J Lipid Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19351971/" }, { "id": "PMID:18772128", "title": "Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18772128/" }, { "id": "PMID:18458083", "title": "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18458083/" }, { "id": "PMID:18781350", "title": "Substrate preferences of a lysophosphatidylcholine acyltransferase highlight its role in phospholipid remodeling", "journal": "Lipids", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18781350/" } ], "x": 2381.3333333333335, "y": 1695.527397260274, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482679", "label": "PA is hydrolysed to 1-acyl LPA by PLA2G2A", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482679", "summary": "PA is hydrolysed to 1-acyl LPA by PLA2G2A is a Reactome release 97 curated reaction event. The source record connects PA, H2O to 1-acyl LPA, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PA + H2O → 1-acyl LPA + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426755", "reactome_id": "R-ALL-426755", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1500604", "reactome_id": "R-ALL-1500604", "label": "1-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2G2A:Ca2+ [endoplasmic reticulum membrane]", "activity_id": "1500631", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" } ], "x": 2660, "y": 1695.527397260274, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482685", "label": "1-acyl LPC is hydrolyzed to GPCho by PLA2[8]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482685", "summary": "1-acyl LPC is hydrolyzed to GPCho by PLA2[8] is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPC, H2O to GPCho, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "1-acyl LPC + H2O → GPCho + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1524028", "reactome_id": "R-ALL-1524028", "label": "1-acyl LPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498750", "reactome_id": "R-ALL-1498750", "label": "GPCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16870", "formula": "C8H20NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine lysophospholipase A1 activity of PLA2(8) [cytosol]", "activity_id": "1524022", "ec_numbers": [ "3.1.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:19019078", "title": "The identification of a phospholipase B precursor in human neutrophils", "journal": "FEBS J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19019078/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:8083230", "title": "Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8083230/" }, { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" }, { "id": "PMID:15944408", "title": "Roles of C-terminal processing, and involvement in transacylation reaction of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "J Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15944408/" } ], "x": 2938.666666666667, "y": 1695.527397260274, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482689", "label": "1-acyl LPG is acylated to PG by CRLS1 (IM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482689", "summary": "1-acyl LPG is acylated to PG by CRLS1 (IM) is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPG, acyl-CoA to PG, CoA-SH in mitochondrial inner membrane · mitochondrial matrix.", "equation": "1-acyl LPG + acyl-CoA → PG + CoA-SH", "inputs": [ { "node_id": "R-ALL-1524027", "reactome_id": "R-ALL-1524027", "label": "1-acyl LPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62747", "formula": "C7H14O9PR", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524038", "reactome_id": "R-ALL-1524038", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524042", "reactome_id": "R-ALL-1524042", "label": "PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of CRLS1 [mitochondrial inner membrane]", "activity_id": "1524025", "ec_numbers": [ "2.3.1.51" ], "accessions": [ "Q9UJA2" ] } ], "citations": [ { "id": "PMID:20025994", "title": "A novel function of the human CLS1 in phosphatidylglycerol synthesis and remodeling", "journal": "Biochim Biophys Acta", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20025994/" } ], "x": 2381.3333333333335, "y": 1700.8561643835617, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482691", "label": "2-acyl LPS is acylated to PS by LPSAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482691", "summary": "2-acyl LPS is acylated to PS by LPSAT is a Reactome release 97 curated reaction event. The source record connects 2-acyl LPS, acyl-CoA to PS, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "2-acyl LPS + acyl-CoA → PS + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498722", "reactome_id": "R-ALL-1498722", "label": "2-acyl LPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37646", "formula": "C7H13NO9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-acylglycerol-3-phosphate O-acyltransferase activity of LPSAT [endoplasmic reticulum membrane]", "activity_id": "1524049", "ec_numbers": [ "2.3.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:18287005", "title": "Discovery of a lysophospholipid acyltransferase family essential for membrane asymmetry and diversity", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18287005/" }, { "id": "PMID:18772128", "title": "Lysophospholipid acyltransferases and arachidonate recycling in human neutrophils", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18772128/" }, { "id": "PMID:18458083", "title": "Molecular identification of a novel mammalian brain isoform of acyl-CoA:lysophospholipid acyltransferase with prominent ethanolamine lysophospholipid acylating activity, LPEAT2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18458083/" } ], "x": 2660, "y": 1700.8561643835617, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482695", "label": "G3P is acylated to 1-acyl LPA by GPAM/GPAT2 (OM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial outer membrane · cytosol", "compartment_ids": [ "GO:0005741", "GO:0005829" ], "rhea_ids": [ "RHEA:15325" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482695", "summary": "G3P is acylated to 1-acyl LPA by GPAM/GPAT2 (OM) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects G3P, acyl-CoA to 1-acyl LPA, CoA-SH in mitochondrial outer membrane · cytosol and cross-references RHEA:15325.", "equation": "G3P + acyl-CoA → 1-acyl LPA + CoA-SH", "inputs": [ { "node_id": "R-ALL-76114", "reactome_id": "R-ALL-76114", "label": "G3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500580", "reactome_id": "R-ALL-1500580", "label": "1-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycerol-3-phosphate O-acyltransferase activity of GPAM/GPAT2 [mitochondrial outer membrane]", "activity_id": "1500616", "ec_numbers": [ "2.3.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:18238778", "title": "AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18238778/" }, { "id": "PMID:18718904", "title": "Acyl-CoA:lysophospholipid acyltransferases", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18718904/" }, { "id": "PMID:19336658", "title": "Biochemistry, physiology, and genetics of GPAT, AGPAT, and lipin enzymes in triglyceride synthesis", "journal": "Am J Physiol Endocrinol Metab", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19336658/" } ], "x": 2938.666666666667, "y": 1551.650684931507, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482696", "label": "1-acyl LPC is hydrolyzed to GPCho by PLA2G4C", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482696", "summary": "1-acyl LPC is hydrolyzed to GPCho by PLA2G4C is a Reactome release 97 curated reaction event. The source record connects 1-acyl LPC, H2O to GPCho, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "1-acyl LPC + H2O → GPCho + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1524028", "reactome_id": "R-ALL-1524028", "label": "1-acyl LPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498750", "reactome_id": "R-ALL-1498750", "label": "GPCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16870", "formula": "C8H20NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine lysophospholipase A1 activity of PLA2G4C [endoplasmic reticulum membrane]", "activity_id": "1524030", "ec_numbers": [ "3.1.1.5" ], "accessions": [ "Q9UP65" ] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" }, { "id": "PMID:15944408", "title": "Roles of C-terminal processing, and involvement in transacylation reaction of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "J Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15944408/" } ], "x": 2938.666666666667, "y": 1700.8561643835617, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482745", "label": "PG is hydrolyzed to 1-acyl LPG by PLA2G4B (IM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482745", "summary": "PG is hydrolyzed to 1-acyl LPG by PLA2G4B (IM) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PG, H2O to 1-acyl LPG, LCFA(-) in mitochondrial inner membrane · mitochondrial matrix.", "equation": "PG + H2O → 1-acyl LPG + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1524042", "reactome_id": "R-ALL-1524042", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1524027", "reactome_id": "R-ALL-1524027", "label": "1-acyl LPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62747", "formula": "C7H14O9PR", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524085", "reactome_id": "R-ALL-1524085", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2G [mitochondrial inner membrane]", "activity_id": "1524136", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2381.3333333333335, "y": 1706.1849315068494, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482759", "label": "MLCL is hydrolyzed to DLCL by PLA2G4A (IM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482759", "summary": "MLCL is hydrolyzed to DLCL by PLA2G4A (IM) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects MLCL, H2O to DLCL, LCFA(-) in mitochondrial inner membrane · mitochondrial matrix.", "equation": "MLCL + H2O → DLCL + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1498773", "reactome_id": "R-ALL-1498773", "label": "MLCL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498753", "reactome_id": "R-ALL-1498753", "label": "DLCL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:75137", "formula": "C11H18O15P2R2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524085", "reactome_id": "R-ALL-1524085", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2G4A:Ca2+ [mitochondrial inner membrane]", "activity_id": "1524096", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:9487141", "title": "Cardiolipin hydrolysis by human phospholipases A2. The multiple enzymatic activities of human cytosolic phospholipase A2", "journal": "Biochim Biophys Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9487141/" }, { "id": "PMID:8083230", "title": "Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8083230/" } ], "x": 2660, "y": 1706.1849315068494, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482771", "label": "PS is hydrolyzed to 1-acyl LPS by PLA2[9]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482771", "summary": "PS is hydrolyzed to 1-acyl LPS by PLA2[9] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PS, H2O to 1-acyl LPS, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PS + H2O → 1-acyl LPS + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498724", "reactome_id": "R-ALL-1498724", "label": "1-acyl LPS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:52603", "formula": "C7H13NO9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(9) [cytosol]", "activity_id": "1524153", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:18614531", "title": "Identification and functional characterization of adipose-specific phospholipase A2 (AdPLA)", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18614531/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2938.666666666667, "y": 1706.1849315068494, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482773", "label": "MLCL transports from the IM to the ER", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005743", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482773", "summary": "MLCL transports from the IM to the ER is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MLCL to MLCL in mitochondrial inner membrane · endoplasmic reticulum membrane.", "equation": "MLCL → MLCL", "inputs": [ { "node_id": "R-ALL-1498773", "reactome_id": "R-ALL-1498773", "label": "MLCL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524069", "reactome_id": "R-ALL-1524069", "label": "MLCL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19737925", "title": "Identification of the human mitochondrial linoleoyl-coenzyme A monolysocardiolipin acyltransferase (MLCL AT-1)", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19737925/" }, { "id": "PMID:19075029", "title": "The microsomal cardiolipin remodeling enzyme acyl-CoA lysocardiolipin acyltransferase is an acyltransferase of multiple anionic lysophospholipids", "journal": "J Lipid Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19075029/" }, { "id": "PMID:15152008", "title": "A novel cardiolipin-remodeling pathway revealed by a gene encoding an endoplasmic reticulum-associated acyl-CoA:lysocardiolipin acyltransferase (ALCAT1) in mouse", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15152008/" } ], "x": 2381.3333333333335, "y": 1903.349315068493, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1482775", "label": "MLCL is acylated to CL by HADH (IM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482775", "summary": "MLCL is acylated to CL by HADH (IM) is a Reactome release 97 curated reaction event. The source record connects MLCL, acyl-CoA to cardiolipin, CoA-SH in mitochondrial inner membrane · mitochondrial matrix.", "equation": "MLCL + acyl-CoA → cardiolipin + CoA-SH", "inputs": [ { "node_id": "R-ALL-1498773", "reactome_id": "R-ALL-1498773", "label": "MLCL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524038", "reactome_id": "R-ALL-1524038", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyltransferase activity, transferring groups other than amino-acyl groups of HADH octamer [mitochondrial inner membrane]", "activity_id": "1524104", "ec_numbers": [ "2.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:19737925", "title": "Identification of the human mitochondrial linoleoyl-coenzyme A monolysocardiolipin acyltransferase (MLCL AT-1)", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19737925/" } ], "x": 2381.3333333333335, "y": 1711.513698630137, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482776", "label": "PS is hydrolyzed to 1-acyl LPS by PLA2G2A", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482776", "summary": "PS is hydrolyzed to 1-acyl LPS by PLA2G2A is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PS, H2O to 1-acyl LPS, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PS + H2O → 1-acyl LPS + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498724", "reactome_id": "R-ALL-1498724", "label": "1-acyl LPS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:52603", "formula": "C7H13NO9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2G2A:Ca2+ [endoplasmic reticulum membrane]", "activity_id": "1500631", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" } ], "x": 2660, "y": 1711.513698630137, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482777", "label": "TAG is hydrolyzed to DAG by PNPLA2/3", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482777", "summary": "TAG is hydrolyzed to DAG by PNPLA2/3 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects TAG, H2O to DAG, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "TAG + H2O → DAG + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500594", "reactome_id": "R-ALL-1500594", "label": "TAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "triacylglycerol lipase activity of PNPLA2/3 [endoplasmic reticulum membrane]", "activity_id": "1500645", "ec_numbers": [ "3.1.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:15364929", "title": "Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15364929/" }, { "id": "PMID:20034933", "title": "A sequence variation (I148M) in PNPLA3 associated with nonalcoholic fatty liver disease disrupts triglyceride hydrolysis", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20034933/" }, { "id": "PMID:21068004", "title": "Pnpla3/Adiponutrin deficiency in mice does not contribute to fatty liver disease or metabolic syndrome", "journal": "J Lipid Res", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21068004/" } ], "x": 2938.666666666667, "y": 1711.513698630137, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482778", "label": "CL is hydrolyzed to MLCL by PLA2G6 (IM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482778", "summary": "CL is hydrolyzed to MLCL by PLA2G6 (IM) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects cardiolipin, H2O to MLCL, LCFA(-) in mitochondrial inner membrane · mitochondrial matrix.", "equation": "cardiolipin + H2O → MLCL + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498773", "reactome_id": "R-ALL-1498773", "label": "MLCL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524085", "reactome_id": "R-ALL-1524085", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2G6 [mitochondrial inner membrane]", "activity_id": "1524077", "ec_numbers": [ "3.1.1.4" ], "accessions": [ "O60733" ] } ], "citations": [ { "id": "PMID:9417066", "title": "Multiple splice variants of the human calcium-independent phospholipase A2 and their effect on enzyme activity", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9417066/" }, { "id": "PMID:10092647", "title": "Human pancreatic islets express mRNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on chromosome 22q13.1", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10092647/" }, { "id": "PMID:10336645", "title": "The human calcium-independent phospholipase A2 gene multiple enzymes with distinct properties from a single gene", "journal": "Eur J Biochem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10336645/" } ], "x": 2381.3333333333335, "y": 1716.8424657534247, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482781", "label": "MLCL and PC are converted to CL and 1-acyl LPC by TAZ (IM) (Reversible)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482781", "summary": "MLCL and PC are converted to CL and 1-acyl LPC by TAZ (IM) (Reversible) is a Reactome release 97 curated reaction event. The source record connects MLCL, PC to cardiolipin, 1-acyl LPC in mitochondrial inner membrane.", "equation": "MLCL + PC → cardiolipin + 1-acyl LPC", "inputs": [ { "node_id": "R-ALL-1498773", "reactome_id": "R-ALL-1498773", "label": "MLCL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524075", "reactome_id": "R-ALL-1524075", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524079", "reactome_id": "R-ALL-1524079", "label": "1-acyl LPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyltransferase activity, transferring groups other than amino-acyl groups of TAZ [mitochondrial inner membrane]", "activity_id": "1524093", "ec_numbers": [ "2.3.1" ], "accessions": [ "Q16635" ] } ], "citations": [ { "id": "PMID:14551214", "title": "Remodeling of cardiolipin by phospholipid transacylation", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14551214/" }, { "id": "PMID:17082194", "title": "The enzymatic function of tafazzin", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17082194/" }, { "id": "PMID:19416660", "title": "Formation of molecular species of mitochondrial cardiolipin. 1. A novel transacylation mechanism to shuttle fatty acids between sn-1 and sn-2 positions of multiple phospholipid species", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19416660/" } ], "x": 2660, "y": 1716.8424657534247, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482794", "label": "CL and 1-acyl LPC are converted to MLCL and PC by TAZ (IM) (Reversible)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482794", "summary": "CL and 1-acyl LPC are converted to MLCL and PC by TAZ (IM) (Reversible) is a Reactome release 97 curated reaction event. The source record connects cardiolipin, 1-acyl LPC to MLCL, PC in mitochondrial inner membrane.", "equation": "cardiolipin + 1-acyl LPC → MLCL + PC", "inputs": [ { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524079", "reactome_id": "R-ALL-1524079", "label": "1-acyl LPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1498773", "reactome_id": "R-ALL-1498773", "label": "MLCL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524075", "reactome_id": "R-ALL-1524075", "label": "PC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-acylglycerophosphocholine O-acyltransferase activity of TAZ [mitochondrial inner membrane]", "activity_id": "1524068", "ec_numbers": [ "2.3.1.23" ], "accessions": [ "Q16635" ] } ], "citations": [ { "id": "PMID:14551214", "title": "Remodeling of cardiolipin by phospholipid transacylation", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14551214/" }, { "id": "PMID:17082194", "title": "The enzymatic function of tafazzin", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17082194/" }, { "id": "PMID:19416660", "title": "Formation of molecular species of mitochondrial cardiolipin. 1. A novel transacylation mechanism to shuttle fatty acids between sn-1 and sn-2 positions of multiple phospholipid species", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19416660/" } ], "x": 2938.666666666667, "y": 1716.8424657534247, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482811", "label": "DAG is hydrolyzed to 2-MAG by PNPLA2/3", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482811", "summary": "DAG is hydrolyzed to 2-MAG by PNPLA2/3 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects DAG, H2O to 2-MAG, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "DAG + H2O → 2-MAG + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498728", "reactome_id": "R-ALL-1498728", "label": "2-MAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17389", "formula": "C4H7O4R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lipoprotein lipase activity of PNPLA2/3 [endoplasmic reticulum membrane]", "activity_id": "1500597", "ec_numbers": [ "3.1.1.34" ], "accessions": [] } ], "citations": [ { "id": "PMID:15364929", "title": "Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15364929/" }, { "id": "PMID:20034933", "title": "A sequence variation (I148M) in PNPLA3 associated with nonalcoholic fatty liver disease disrupts triglyceride hydrolysis", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20034933/" }, { "id": "PMID:21068004", "title": "Pnpla3/Adiponutrin deficiency in mice does not contribute to fatty liver disease or metabolic syndrome", "journal": "J Lipid Res", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21068004/" } ], "x": 2381.3333333333335, "y": 1722.1712328767123, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482816", "label": "PC is hydrolyzed to 1-acyl LPC by PLA2[6]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482816", "summary": "PC is hydrolyzed to 1-acyl LPC by PLA2[6] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PC, H2O to 1-acyl LPC, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PC + H2O → 1-acyl LPC + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1524028", "reactome_id": "R-ALL-1524028", "label": "1-acyl LPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(6) [endoplasmic reticulum membrane]", "activity_id": "1524115", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:2925608", "title": "Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2925608/" }, { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:9705332", "title": "A novel calcium-independent phospholipase A2, cPLA2-gamma, that is prenylated and contains homology to cPLA2", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9705332/" }, { "id": "PMID:10085124", "title": "Molecular cloning of two new human paralogs of 85-kDa cytosolic phospholipase A2", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10085124/" }, { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" } ], "x": 2660, "y": 1722.1712328767123, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482825", "label": "PI is hydrolyzed to 1-acyl LPI by PLA2[11]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482825", "summary": "PI is hydrolyzed to 1-acyl LPI by PLA2[11] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PI, H2O to 1-acyl LPI, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PI + H2O → 1-acyl LPI + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498733", "reactome_id": "R-ALL-1498733", "label": "1-acyl LPI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28914", "formula": "C10H18O12PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(11) [cytosol]", "activity_id": "1524149", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:19019078", "title": "The identification of a phospholipase B precursor in human neutrophils", "journal": "FEBS J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19019078/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:18614531", "title": "Identification and functional characterization of adipose-specific phospholipase A2 (AdPLA)", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18614531/" } ], "x": 2938.666666666667, "y": 1722.1712328767123, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482827", "label": "PC is hydrolyzed to 2-acyl LPC by PLA2G4C", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482827", "summary": "PC is hydrolyzed to 2-acyl LPC by PLA2G4C is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PC, H2O to 2-acyl LPC, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PC + H2O → 2-acyl LPC + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-975614", "reactome_id": "R-ALL-975614", "label": "2-acyl LPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16728", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of PLA2G4C [endoplasmic reticulum membrane]", "activity_id": "1524121", "ec_numbers": [ "3.1.1.32" ], "accessions": [ "Q9UP65" ] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" }, { "id": "PMID:15944408", "title": "Roles of C-terminal processing, and involvement in transacylation reaction of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "J Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15944408/" } ], "x": 2381.3333333333335, "y": 1727.5, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482828", "label": "PE is hydrolyzed to 2-acyl LPE by PLA2[4]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482828", "summary": "PE is hydrolyzed to 2-acyl LPE by PLA2[4] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PE, H2O to 2-acyl LPE, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PE + H2O → 2-acyl LPE + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498729", "reactome_id": "R-ALL-1498729", "label": "2-acyl LPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28936", "formula": "C6H13NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of PLA2(4) [cytosol]", "activity_id": "1524134", "ec_numbers": [ "3.1.1.32" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2660, "y": 1727.5, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482847", "label": "PG is hydrolysed to 2-acyl LPG by PLA2G4B (IM)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482847", "summary": "PG is hydrolysed to 2-acyl LPG by PLA2G4B (IM) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PG, H2O to 2-acyl LPG, LCFA(-) in mitochondrial inner membrane · mitochondrial matrix.", "equation": "PG + H2O → 2-acyl LPG + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1524042", "reactome_id": "R-ALL-1524042", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1524047", "reactome_id": "R-ALL-1524047", "label": "2-acyl LPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27923", "formula": "C7H14O9PR", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524085", "reactome_id": "R-ALL-1524085", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of PLA2G [mitochondrial inner membrane]", "activity_id": "1524148", "ec_numbers": [ "3.1.1.32" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2938.666666666667, "y": 1727.5, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482850", "label": "MLCL and PE are converted to CL and 1-acyl LPE by TAZ (IM) (Reversible)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482850", "summary": "MLCL and PE are converted to CL and 1-acyl LPE by TAZ (IM) (Reversible) is a Reactome release 97 curated reaction event. The source record connects MLCL, PE to cardiolipin, 1-acyl LPE in mitochondrial inner membrane.", "equation": "MLCL + PE → cardiolipin + 1-acyl LPE", "inputs": [ { "node_id": "R-ALL-1498773", "reactome_id": "R-ALL-1498773", "label": "MLCL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524083", "reactome_id": "R-ALL-1524083", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524089", "reactome_id": "R-ALL-1524089", "label": "1-acyl LPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29017", "formula": "C6H13NO7PR", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyltransferase activity, transferring groups other than amino-acyl groups of TAZ [mitochondrial inner membrane]", "activity_id": "1524093", "ec_numbers": [ "2.3.1" ], "accessions": [ "Q16635" ] } ], "citations": [ { "id": "PMID:14551214", "title": "Remodeling of cardiolipin by phospholipid transacylation", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14551214/" }, { "id": "PMID:17082194", "title": "The enzymatic function of tafazzin", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17082194/" }, { "id": "PMID:19416660", "title": "Formation of molecular species of mitochondrial cardiolipin. 1. A novel transacylation mechanism to shuttle fatty acids between sn-1 and sn-2 positions of multiple phospholipid species", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19416660/" } ], "x": 2381.3333333333335, "y": 1732.8287671232877, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482856", "label": "PC is hydrolyzed to 1-acyl LPC by PLA2[5]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482856", "summary": "PC is hydrolyzed to 1-acyl LPC by PLA2[5] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PC, H2O to 1-acyl LPC, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PC + H2O → 1-acyl LPC + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1524028", "reactome_id": "R-ALL-1524028", "label": "1-acyl LPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(5) [cytosol]", "activity_id": "1524124", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:19019078", "title": "The identification of a phospholipase B precursor in human neutrophils", "journal": "FEBS J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19019078/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:1904318", "title": "A novel arachidonic acid-selective cytosolic PLA2 contains a Ca(2+)-dependent translocation domain with homology to PKC and GAP", "journal": "Cell", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1904318/" }, { "id": "PMID:8083230", "title": "Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8083230/" }, { "id": "PMID:10358058", "title": "Molecular characterization of cytosolic phospholipase A2-beta", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10358058/" }, { "id": "PMID:9705332", "title": "A novel calcium-independent phospholipase A2, cPLA2-gamma, that is prenylated and contains homology to cPLA2", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9705332/" } ], "x": 2660, "y": 1732.8287671232877, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482857", "label": "CL transports from the ER to the IM", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · mitochondrial inner membrane", "compartment_ids": [ "GO:0005789", "GO:0005743" ], "rhea_ids": [ "RHEA:38695" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482857", "summary": "CL transports from the ER to the IM is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CL to cardiolipin in endoplasmic reticulum membrane · mitochondrial inner membrane and cross-references RHEA:38695.", "equation": "CL → cardiolipin", "inputs": [ { "node_id": "R-ALL-1524081", "reactome_id": "R-ALL-1524081", "label": "CL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19075029", "title": "The microsomal cardiolipin remodeling enzyme acyl-CoA lysocardiolipin acyltransferase is an acyltransferase of multiple anionic lysophospholipids", "journal": "J Lipid Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19075029/" }, { "id": "PMID:2037561", "title": "Mitochondrial membrane contact sites of yeast. Characterization of lipid components and possible involvement in intramitochondrial translocation of phospholipids", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2037561/" }, { "id": "PMID:7744750", "title": "Evidence that phosphatidylserine is imported into mitochondria via a mitochondria-associated membrane and that the majority of mitochondrial phosphatidylethanolamine is derived from decarboxylation of phosphatidylserine", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7744750/" }, { "id": "PMID:7696296", "title": "Characterization of a microsomal subfraction associated with mitochondria of the yeast, Saccharomyces cerevisiae. Involvement in synthesis and import of phospholipids into mitochondria", "journal": "Biochim Biophys Acta", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7696296/" }, { "id": "PMID:8245031", "title": "Involvement of mitochondrial contact sites in the subcellular compartmentalization of phospholipid biosynthetic enzymes", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8245031/" }, { "id": "PMID:2332429", "title": "Phospholipid synthesis in a membrane fraction associated with mitochondria", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2332429/" } ], "x": 2660, "y": 1668.8835616438357, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1482860", "label": "DLCL transports from the IM to the ER", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005743", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482860", "summary": "DLCL transports from the IM to the ER is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DLCL to DLCL in mitochondrial inner membrane · endoplasmic reticulum membrane.", "equation": "DLCL → DLCL", "inputs": [ { "node_id": "R-ALL-1498753", "reactome_id": "R-ALL-1498753", "label": "DLCL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:75137", "formula": "C11H18O15P2R2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524080", "reactome_id": "R-ALL-1524080", "label": "DLCL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:75137", "formula": "C11H18O15P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9487141", "title": "Cardiolipin hydrolysis by human phospholipases A2. The multiple enzymatic activities of human cytosolic phospholipase A2", "journal": "Biochim Biophys Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9487141/" }, { "id": "PMID:19075029", "title": "The microsomal cardiolipin remodeling enzyme acyl-CoA lysocardiolipin acyltransferase is an acyltransferase of multiple anionic lysophospholipids", "journal": "J Lipid Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19075029/" } ], "x": 2660, "y": 1903.349315068493, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1482861", "label": "MLCL is acylated to CL by LCLAT1 (ER)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482861", "summary": "MLCL is acylated to CL by LCLAT1 (ER) is a Reactome release 97 curated reaction event. The source record connects MLCL, acyl-CoA to CL, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "MLCL + acyl-CoA → CL + CoA-SH", "inputs": [ { "node_id": "R-ALL-1524069", "reactome_id": "R-ALL-1524069", "label": "MLCL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524081", "reactome_id": "R-ALL-1524081", "label": "CL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyltransferase activity, transferring groups other than amino-acyl groups of LCLAT1 [endoplasmic reticulum membrane]", "activity_id": "1524086", "ec_numbers": [ "2.3.1" ], "accessions": [ "Q6UWP7" ] } ], "citations": [ { "id": "PMID:19075029", "title": "The microsomal cardiolipin remodeling enzyme acyl-CoA lysocardiolipin acyltransferase is an acyltransferase of multiple anionic lysophospholipids", "journal": "J Lipid Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19075029/" }, { "id": "PMID:15152008", "title": "A novel cardiolipin-remodeling pathway revealed by a gene encoding an endoplasmic reticulum-associated acyl-CoA:lysocardiolipin acyltransferase (ALCAT1) in mouse", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15152008/" } ], "x": 2938.666666666667, "y": 1732.8287671232877, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482862", "label": "PC is hydrolysed to 2-acyl LPC by PLA2[7]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482862", "summary": "PC is hydrolysed to 2-acyl LPC by PLA2[7] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PC, H2O to 2-acyl LPC, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PC + H2O → 2-acyl LPC + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-975614", "reactome_id": "R-ALL-975614", "label": "2-acyl LPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16728", "formula": "C9H20NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of PLA2(7) [cytosol]", "activity_id": "1524123", "ec_numbers": [ "3.1.1.32" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2381.3333333333335, "y": 1738.1575342465753, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482867", "label": "DLCL is acylated to MLCL by LCLAT1 (ER)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482867", "summary": "DLCL is acylated to MLCL by LCLAT1 (ER) is a Reactome release 97 curated reaction event. The source record connects DLCL, acyl-CoA to MLCL, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "DLCL + acyl-CoA → MLCL + CoA-SH", "inputs": [ { "node_id": "R-ALL-1524080", "reactome_id": "R-ALL-1524080", "label": "DLCL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:75137", "formula": "C11H18O15P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524069", "reactome_id": "R-ALL-1524069", "label": "MLCL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyltransferase activity, transferring groups other than amino-acyl groups of LCLAT1 [endoplasmic reticulum membrane]", "activity_id": "1524086", "ec_numbers": [ "2.3.1" ], "accessions": [ "Q6UWP7" ] } ], "citations": [ { "id": "PMID:19075029", "title": "The microsomal cardiolipin remodeling enzyme acyl-CoA lysocardiolipin acyltransferase is an acyltransferase of multiple anionic lysophospholipids", "journal": "J Lipid Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19075029/" } ], "x": 2660, "y": 1738.1575342465753, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482868", "label": "PI is hydrolyzed to 1-acyl LPI by PLA2[12]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482868", "summary": "PI is hydrolyzed to 1-acyl LPI by PLA2[12] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PI, H2O to 1-acyl LPI, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PI + H2O → 1-acyl LPI + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498733", "reactome_id": "R-ALL-1498733", "label": "1-acyl LPI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28914", "formula": "C10H18O12PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(12) [endoplasmic reticulum membrane]", "activity_id": "1524144", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2938.666666666667, "y": 1738.1575342465753, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482884", "label": "PE is hydrolyzed to 1-acyl LPE by PLA2[2]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482884", "summary": "PE is hydrolyzed to 1-acyl LPE by PLA2[2] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PE, H2O to 1-acyl LPE, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PE + H2O → 1-acyl LPE + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498738", "reactome_id": "R-ALL-1498738", "label": "1-acyl LPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29017", "formula": "C6H13NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(2) [cytosol]", "activity_id": "1524129", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:19019078", "title": "The identification of a phospholipase B precursor in human neutrophils", "journal": "FEBS J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19019078/" }, { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:18614531", "title": "Identification and functional characterization of adipose-specific phospholipase A2 (AdPLA)", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18614531/" }, { "id": "PMID:9417066", "title": "Multiple splice variants of the human calcium-independent phospholipase A2 and their effect on enzyme activity", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9417066/" }, { "id": "PMID:10092647", "title": "Human pancreatic islets express mRNA species encoding two distinct catalytically active isoforms of group VI phospholipase A2 (iPLA2) that arise from an exon-skipping mechanism of alternative splicing of the transcript from the iPLA2 gene on chromosome 22q13.1", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10092647/" }, { "id": "PMID:10336645", "title": "The human calcium-independent phospholipase A2 gene multiple enzymes with distinct properties from a single gene", "journal": "Eur J Biochem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10336645/" } ], "x": 2381.3333333333335, "y": 1743.486301369863, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482887", "label": "PE is hydrolyzed to 1-acyl LPE by PLA2[3]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482887", "summary": "PE is hydrolyzed to 1-acyl LPE by PLA2[3] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PE, H2O to 1-acyl LPE, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PE + H2O → 1-acyl LPE + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498738", "reactome_id": "R-ALL-1498738", "label": "1-acyl LPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29017", "formula": "C6H13NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(3) [endoplasmic reticulum membrane]", "activity_id": "1524133", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:2925633", "title": "Structure and properties of a human non-pancreatic phospholipase A2", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2925633/" }, { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:15695510", "title": "Group VIB Ca2+-independent phospholipase A2gamma promotes cellular membrane hydrolysis and prostaglandin production in a manner distinct from other intracellular phospholipases A2", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15695510/" } ], "x": 2660, "y": 1743.486301369863, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482889", "label": "DAG is acylated to TAG by DGAT1/2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482889", "summary": "DAG is acylated to TAG by DGAT1/2 is a Reactome release 97 curated reaction event. The source record connects DAG, acyl-CoA to TAG, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "DAG + acyl-CoA → TAG + CoA-SH", "inputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500594", "reactome_id": "R-ALL-1500594", "label": "TAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diacylglycerol O-acyltransferase activity of DGAT1/2 [endoplasmic reticulum membrane]", "activity_id": "1500598", "ec_numbers": [ "2.3.1.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:9756920", "title": "Characterization of two human genes encoding acyl coenzyme A:cholesterol acyltransferase-related enzymes", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9756920/" }, { "id": "PMID:11481335", "title": "Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11481335/" }, { "id": "PMID:14521909", "title": "A novel diacylglycerol acyltransferase (DGAT2) is decreased in human psoriatic skin and increased in diabetic mice", "journal": "Biochem Biophys Res Commun", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14521909/" } ], "x": 2938.666666666667, "y": 1743.486301369863, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482892", "label": "PE is hydrolyzed to 2-acyl LPE by PLA2G4C", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482892", "summary": "PE is hydrolyzed to 2-acyl LPE by PLA2G4C is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PE, H2O to 2-acyl LPE, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PE + H2O → 2-acyl LPE + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498729", "reactome_id": "R-ALL-1498729", "label": "2-acyl LPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28936", "formula": "C6H13NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of PLA2G4C [endoplasmic reticulum membrane]", "activity_id": "1524121", "ec_numbers": [ "3.1.1.32" ], "accessions": [ "Q9UP65" ] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" }, { "id": "PMID:19501189", "title": "Subcellular localization and lysophospholipase/transacylation activities of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19501189/" }, { "id": "PMID:15944408", "title": "Roles of C-terminal processing, and involvement in transacylation reaction of human group IVC phospholipase A2 (cPLA2gamma)", "journal": "J Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15944408/" } ], "x": 2381.3333333333335, "y": 1748.8150684931506, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482894", "label": "CL and 1-acyl LPE are converted to MLCL and PE by TAZ (IM) (Reversible)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482894", "summary": "CL and 1-acyl LPE are converted to MLCL and PE by TAZ (IM) (Reversible) is a Reactome release 97 curated reaction event. The source record connects cardiolipin, 1-acyl LPE to MLCL, PE in mitochondrial inner membrane.", "equation": "cardiolipin + 1-acyl LPE → MLCL + PE", "inputs": [ { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524089", "reactome_id": "R-ALL-1524089", "label": "1-acyl LPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29017", "formula": "C6H13NO7PR", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1498773", "reactome_id": "R-ALL-1498773", "label": "MLCL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60430", "formula": "C12H19O16P2R3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524083", "reactome_id": "R-ALL-1524083", "label": "PE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of TAZ [mitochondrial inner membrane]", "activity_id": "1524084", "ec_numbers": [ "2.3.1.51" ], "accessions": [ "Q16635" ] } ], "citations": [ { "id": "PMID:14551214", "title": "Remodeling of cardiolipin by phospholipid transacylation", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14551214/" }, { "id": "PMID:17082194", "title": "The enzymatic function of tafazzin", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17082194/" }, { "id": "PMID:19416660", "title": "Formation of molecular species of mitochondrial cardiolipin. 1. A novel transacylation mechanism to shuttle fatty acids between sn-1 and sn-2 positions of multiple phospholipid species", "journal": "Biochim Biophys Acta", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19416660/" } ], "x": 2660, "y": 1748.8150684931506, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482897", "label": "PS is hydrolyzed to 2-acyl LPS by PLA2[10]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482897", "summary": "PS is hydrolyzed to 2-acyl LPS by PLA2[10] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PS, H2O to 2-acyl LPS, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PS + H2O → 2-acyl LPS + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498722", "reactome_id": "R-ALL-1498722", "label": "2-acyl LPS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:37646", "formula": "C7H13NO9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(10) [cytosol]", "activity_id": "1524152", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2938.666666666667, "y": 1748.8150684931506, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482900", "label": "PG is hydrolyzed to 1-acyl LPG by PLA2[1]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482900", "summary": "PG is hydrolyzed to 1-acyl LPG by PLA2[1] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PG, H2O to 1-acyl LPG, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PG + H2O → 1-acyl LPG + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1483256", "reactome_id": "R-ALL-1483256", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498740", "reactome_id": "R-ALL-1498740", "label": "1-acyl LPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62747", "formula": "C7H14O9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(1) [cytosol]", "activity_id": "1500640", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2381.3333333333335, "y": 1754.1438356164383, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482907", "label": "PG is hydrolyzed to 1-acyl LPG by PLA2G2A", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482907", "summary": "PG is hydrolyzed to 1-acyl LPG by PLA2G2A is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PG, H2O to 1-acyl LPG, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PG + H2O → 1-acyl LPG + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1483256", "reactome_id": "R-ALL-1483256", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498740", "reactome_id": "R-ALL-1498740", "label": "1-acyl LPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62747", "formula": "C7H14O9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2G2A:Ca2+ [endoplasmic reticulum membrane]", "activity_id": "1500631", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" } ], "x": 2660, "y": 1754.1438356164383, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482920", "label": "PG is hydrolyzed to 2-acyl LPG by PLA2[14]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482920", "summary": "PG is hydrolyzed to 2-acyl LPG by PLA2[14] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PG, H2O to 2-acyl LPG, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PG + H2O → 2-acyl LPG + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1483256", "reactome_id": "R-ALL-1483256", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498730", "reactome_id": "R-ALL-1498730", "label": "2-acyl LPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27923", "formula": "C7H14O9PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of PLA2(14) [cytosol]", "activity_id": "1524145", "ec_numbers": [ "3.1.1.32" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2938.666666666667, "y": 1754.1438356164383, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482932", "label": "PI is hydrolyzed to 2-acyl LPI by PLA2[13]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482932", "summary": "PI is hydrolyzed to 2-acyl LPI by PLA2[13] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PI, H2O to 2-acyl LPI, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "PI + H2O → 2-acyl LPI + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498737", "reactome_id": "R-ALL-1498737", "label": "2-acyl LPI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62746", "formula": "C10H18O12PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of PLA2(13) [cytosol]", "activity_id": "1524140", "ec_numbers": [ "3.1.1.32" ], "accessions": [] } ], "citations": [ { "id": "PMID:20705608", "title": "Interfacial kinetic and binding properties of mammalian group IVB phospholipase A2 (cPLA2beta) and comparison with the other cPLA2 isoforms", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705608/" } ], "x": 2381.3333333333335, "y": 1759.472602739726, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1482939", "label": "CDP-DAG is converted to PGP by PGS1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:12593" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482939", "summary": "CDP-DAG is converted to PGP by PGS1 is a Reactome release 97 curated reaction event. The source record connects CDP-DAG, G3P to PGP, CMP in mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:12593.", "equation": "CDP-DAG + G3P → PGP + CMP", "inputs": [ { "node_id": "R-ALL-1524064", "reactome_id": "R-ALL-1524064", "label": "CDP-DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58332", "formula": "C14H17N3O15P2R2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-188458", "reactome_id": "R-ALL-188458", "label": "G3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524059", "reactome_id": "R-ALL-1524059", "label": "PGP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60110", "formula": "C8H11O13P2R2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524067", "reactome_id": "R-ALL-1524067", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "CDP-diacylglycerol-glycerol-3-phosphate 3-phosphatidyltransferase activity of PGS1 [mitochondrial inner membrane]", "activity_id": "1524058", "ec_numbers": [ "2.7.8.5" ], "accessions": [ "Q32NB8" ] } ], "citations": [ { "id": "PMID:14702039", "title": "Complete sequencing and characterization of 21,243 full-length human cDNAs", "journal": "Nat Genet", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14702039/" } ], "x": 2381.3333333333335, "y": 1556.9794520547946, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482961", "label": "CDP-Cho and DAG are converted to PC by CEPT1 at the ER membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:32939" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482961", "summary": "CDP-Cho and DAG are converted to PC by CEPT1 at the ER membrane is a Reactome release 97 curated reaction event. The source record connects DAG, CDP-Cho to PC, CMP in endoplasmic reticulum membrane · cytosol and cross-references RHEA:32939.", "equation": "DAG + CDP-Cho → PC + CMP", "inputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524060", "reactome_id": "R-ALL-1524060", "label": "CDP-Cho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16436", "formula": "C14H26N4O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109395", "reactome_id": "R-ALL-109395", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "diacylglycerol cholinephosphotransferase activity of CEPT1:Mg2+/Mn2+ [endoplasmic reticulum membrane]", "activity_id": "1524063", "ec_numbers": [ "2.7.8.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:10191259", "title": "Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine", "journal": "Biochem J", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10191259/" }, { "id": "PMID:10893425", "title": "Cloning, genomic organization, and characterization of a human cholinephosphotransferase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10893425/" }, { "id": "PMID:12216837", "title": "PC and PE synthesis: mixed micellar analysis of the cholinephosphotransferase and ethanolaminephosphotransferase activities of human choline/ethanolamine phosphotransferase 1 (CEPT1)", "journal": "Lipids", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12216837/" }, { "id": "PMID:12221122", "title": "The major sites of cellular phospholipid synthesis and molecular determinants of Fatty Acid and lipid head group specificity", "journal": "Mol Biol Cell", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12221122/" } ], "x": 2660, "y": 1556.9794520547946, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482962", "label": "CDP-ETA and DAG are converted to PE by CEPT1/EPT1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1482962", "summary": "CDP-ETA and DAG are converted to PE by CEPT1/EPT1 is a Reactome release 97 curated reaction event. The source record connects DAG, CDP-ETA to PE, CMP in endoplasmic reticulum membrane · cytosol.", "equation": "DAG + CDP-ETA → PE + CMP", "inputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500603", "reactome_id": "R-ALL-1500603", "label": "CDP-ETA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57876", "formula": "C11H19N4O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109395", "reactome_id": "R-ALL-109395", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ethanolaminephosphotransferase activity of CEPT1/EPT1 [endoplasmic reticulum membrane]", "activity_id": "1500586", "ec_numbers": [ "2.7.8.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10191259", "title": "Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine", "journal": "Biochem J", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10191259/" }, { "id": "PMID:10893425", "title": "Cloning, genomic organization, and characterization of a human cholinephosphotransferase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10893425/" }, { "id": "PMID:12216837", "title": "PC and PE synthesis: mixed micellar analysis of the cholinephosphotransferase and ethanolaminephosphotransferase activities of human choline/ethanolamine phosphotransferase 1 (CEPT1)", "journal": "Lipids", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12216837/" }, { "id": "PMID:12221122", "title": "The major sites of cellular phospholipid synthesis and molecular determinants of Fatty Acid and lipid head group specificity", "journal": "Mol Biol Cell", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12221122/" }, { "id": "PMID:17132865", "title": "Identification and characterization of human ethanolaminephosphotransferase1", "journal": "J Lipid Res", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17132865/" } ], "x": 2660, "y": 1759.472602739726, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482973", "label": "CDP-Cho and DAG are converted to PC by CHPT1 at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:32939" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482973", "summary": "CDP-Cho and DAG are converted to PC by CHPT1 at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects CDP-Cho, DAG to CMP, PC in Golgi membrane · cytosol and cross-references RHEA:32939.", "equation": "CDP-Cho + DAG → CMP + PC", "inputs": [ { "node_id": "R-ALL-1524060", "reactome_id": "R-ALL-1524060", "label": "CDP-Cho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16436", "formula": "C14H26N4O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524057", "reactome_id": "R-ALL-1524057", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-109395", "reactome_id": "R-ALL-109395", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-429785", "reactome_id": "R-ALL-429785", "label": "PC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "diacylglycerol cholinephosphotransferase activity of CHPT1:Mg2+/Mn2+ [Golgi membrane]", "activity_id": "1524061", "ec_numbers": [ "2.7.8.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:10191259", "title": "Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine", "journal": "Biochem J", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10191259/" }, { "id": "PMID:10893425", "title": "Cloning, genomic organization, and characterization of a human cholinephosphotransferase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10893425/" }, { "id": "PMID:12216837", "title": "PC and PE synthesis: mixed micellar analysis of the cholinephosphotransferase and ethanolaminephosphotransferase activities of human choline/ethanolamine phosphotransferase 1 (CEPT1)", "journal": "Lipids", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12216837/" }, { "id": "PMID:12221122", "title": "The major sites of cellular phospholipid synthesis and molecular determinants of Fatty Acid and lipid head group specificity", "journal": "Mol Biol Cell", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12221122/" } ], "x": 2938.666666666667, "y": 1556.9794520547946, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1482976", "label": "CDP-DAG is converted to PI by CDIPT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:11580" ], "source_url": "https://reactome.org/content/detail/R-HSA-1482976", "summary": "CDP-DAG is converted to PI by CDIPT is a Reactome release 97 curated reaction event. The source record connects CDP-DAG, Ins to PI, CMP in endoplasmic reticulum membrane · cytosol and cross-references RHEA:11580.", "equation": "CDP-DAG + Ins → PI + CMP", "inputs": [ { "node_id": "R-ALL-426955", "reactome_id": "R-ALL-426955", "label": "CDP-DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58332", "formula": "C14H17N3O15P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-426910", "reactome_id": "R-ALL-426910", "label": "Ins", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17268", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109395", "reactome_id": "R-ALL-109395", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "CDP-diacylglycerol-inositol 3-phosphatidyltransferase activity of CDIPT:Mg2+/Mn2+ [endoplasmic reticulum membrane]", "activity_id": "1524066", "ec_numbers": [ "2.7.8.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:9407135", "title": "The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9407135/" } ], "x": 2381.3333333333335, "y": 1562.3082191780823, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483002", "label": "DHAP is converted to 1-acyl GO3P by GNPAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · peroxisomal membrane", "compartment_ids": [ "GO:0005829", "GO:0005778" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483002", "summary": "DHAP is converted to 1-acyl GO3P by GNPAT is a Reactome release 97 curated reaction event. The source record connects DHAP, acyl-CoA to CoA-SH, GO3P in cytosol · peroxisomal membrane.", "equation": "DHAP + acyl-CoA → CoA-SH + GO3P", "inputs": [ { "node_id": "R-ALL-75970", "reactome_id": "R-ALL-75970", "label": "DHAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57642", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-75973", "reactome_id": "R-ALL-75973", "label": "GO3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73315", "formula": "C3H4O6PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerone-phosphate O-acyltransferase activity of GNPAT [peroxisomal membrane]", "activity_id": "1500614", "ec_numbers": [ "2.3.1.42" ], "accessions": [ "O15228" ] } ], "citations": [ { "id": "PMID:10553003", "title": "Ether lipid biosynthesis: alkyl-dihydroxyacetonephosphate synthase protein deficiency leads to reduced dihydroxyacetonephosphate acyltransferase activities", "journal": "J Lipid Res", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10553003/" }, { "id": "PMID:8186247", "title": "Purification of peroxisomal acyl-CoA: dihydroxyacetonephosphate acyltransferase from human placenta", "journal": "Biochim Biophys Acta", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8186247/" } ], "x": 2938.666666666667, "y": 1759.472602739726, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483004", "label": "Cho is phosphorylated to PCho by CHK dimer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483004", "summary": "Cho is phosphorylated to PCho by CHK dimer is a Reactome release 97 curated reaction event. The source record connects Cho, ATP to PCho, ADP in cytosol.", "equation": "Cho + ATP → PCho + ADP", "inputs": [ { "node_id": "R-ALL-264618", "reactome_id": "R-ALL-264618", "label": "Cho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1524071", "reactome_id": "R-ALL-1524071", "label": "PCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36700", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "choline kinase activity of CHK dimer [cytosol]", "activity_id": "1524073", "ec_numbers": [ "2.7.1.32" ], "accessions": [] } ], "citations": [ { "id": "PMID:19915674", "title": "Differential role of human choline kinase alpha and beta enzymes in lipid metabolism: implications in cancer onset and treatment", "journal": "PLoS One", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19915674/" }, { "id": "PMID:17007874", "title": "Elucidation of human choline kinase crystal structures in complex with the products ADP or phosphocholine", "journal": "J Mol Biol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17007874/" } ], "x": 2381.3333333333335, "y": 1764.8013698630136, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483063", "label": "PG and CDP-DAG are converted to CL by CRLS1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:32931" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483063", "summary": "PG and CDP-DAG are converted to CL by CRLS1 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PG, CDP-DAG to cardiolipin, CMP in mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:32931.", "equation": "PG + CDP-DAG → cardiolipin + CMP", "inputs": [ { "node_id": "R-ALL-1524042", "reactome_id": "R-ALL-1524042", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524064", "reactome_id": "R-ALL-1524064", "label": "CDP-DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58332", "formula": "C14H17N3O15P2R2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524067", "reactome_id": "R-ALL-1524067", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cardiolipin synthase (CMP-forming) activity of CRLS1 [mitochondrial inner membrane]", "activity_id": "1524138", "ec_numbers": [ "2.7.8.41" ], "accessions": [ "Q9UJA2" ] } ], "citations": [ { "id": "PMID:16678169", "title": "Identification and characterization of human cardiolipin synthase", "journal": "FEBS Lett", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16678169/" }, { "id": "PMID:16547353", "title": "Cloning and characterization of a cDNA encoding human cardiolipin synthase (hCLS1)", "journal": "J Lipid Res", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16547353/" } ], "x": 2660, "y": 1562.3082191780823, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1483077", "label": "PE transports from the mitochondrial membrane to the ER", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005743", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483077", "summary": "PE transports from the mitochondrial membrane to the ER is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PE to PE in mitochondrial inner membrane · endoplasmic reticulum membrane.", "equation": "PE → PE", "inputs": [ { "node_id": "R-ALL-1524083", "reactome_id": "R-ALL-1524083", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:2332429", "title": "Phospholipid synthesis in a membrane fraction associated with mitochondria", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2332429/" }, { "id": "PMID:1898727", "title": "Newly made phosphatidylserine and phosphatidylethanolamine are preferentially translocated between rat liver mitochondria and endoplasmic reticulum", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1898727/" } ], "x": 2660, "y": 1892.6917808219177, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1483081", "label": "PCho and CTP are condensed to CDP-Cho by PCYT1 dimer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483081", "summary": "PCho and CTP are condensed to CDP-Cho by PCYT1 dimer is a Reactome release 97 curated reaction event. The source record connects PCho, CTP to CDP-Cho, PPi in endoplasmic reticulum membrane · cytosol.", "equation": "PCho + CTP → CDP-Cho + PPi", "inputs": [ { "node_id": "R-ALL-1524071", "reactome_id": "R-ALL-1524071", "label": "PCho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36700", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-110614", "reactome_id": "R-ALL-110614", "label": "CTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37563", "formula": "C9H12N3O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524060", "reactome_id": "R-ALL-1524060", "label": "CDP-Cho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16436", "formula": "C14H26N4O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "choline-phosphate cytidylyltransferase activity of PCYT1 dimer [endoplasmic reticulum membrane]", "activity_id": "1524105", "ec_numbers": [ "2.7.7.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:9593753", "title": "Cloning and characterization of a second human CTP:phosphocholine cytidylyltransferase", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9593753/" } ], "x": 2660, "y": 1764.8013698630136, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483087", "label": "PI is exchanged with PC by PITPNB", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane", "compartment_ids": [ "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483087", "summary": "PI is exchanged with PC by PITPNB is a Reactome release 97 curated reaction event. The source record connects PI:PITPNB, PC to PI, PC:PITPNB in Golgi membrane.", "equation": "PI:PITPNB + PC → PI + PC:PITPNB", "inputs": [ { "node_id": "R-HSA-1524150", "reactome_id": "R-HSA-1524150", "label": "PI:PITPNB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-429785", "reactome_id": "R-ALL-429785", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524146", "reactome_id": "R-ALL-1524146", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-1524122", "reactome_id": "R-HSA-1524122", "label": "PC:PITPNB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine transfer activity of PI:PITPNB [Golgi membrane]", "activity_id": "9855947", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18636990", "title": "Dynamics of lipid transfer by phosphatidylinositol transfer proteins in cells", "journal": "Traffic", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18636990/" }, { "id": "PMID:20332109", "title": "Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum", "journal": "J Cell Sci", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20332109/" }, { "id": "PMID:11104777", "title": "Structure of a multifunctional protein. Mammalian phosphatidylinositol transfer protein complexed with phosphatidylcholine", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11104777/" }, { "id": "PMID:16274224", "title": "Structure of PITPbeta in complex with phosphatidylcholine: comparison of structure and lipid transfer to other PITP isoforms", "journal": "Biochemistry", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16274224/" }, { "id": "PMID:11980708", "title": "Structure of apo-phosphatidylinositol transfer protein alpha provides insight into membrane association", "journal": "EMBO J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980708/" }, { "id": "PMID:14962392", "title": "Structure-function analysis of human [corrected] phosphatidylinositol transfer protein alpha bound to phosphatidylinositol", "journal": "Structure", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14962392/" } ], "x": 2938.666666666667, "y": 1850.0616438356165, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483089", "label": "PE is converted to PS by PTDSS2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483089", "summary": "PE is converted to PS by PTDSS2 is a Reactome release 97 curated reaction event. The source record connects PE, L-Ser to PS, ETA in endoplasmic reticulum membrane · cytosol.", "equation": "PE + L-Ser → PS + ETA", "inputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1498751", "reactome_id": "R-ALL-1498751", "label": "ETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57603", "formula": "C2H8NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transferase activity of PTDSS2 [endoplasmic reticulum membrane]", "activity_id": "1524132", "ec_numbers": [ "2" ], "accessions": [ "Q9BVG9" ] } ], "citations": [ { "id": "PMID:19014349", "title": "Purification and characterization of human phosphatidylserine synthases 1 and 2", "journal": "Biochem J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19014349/" }, { "id": "PMID:9642289", "title": "Genetic evidence that phosphatidylserine synthase II catalyzes the conversion of phosphatidylethanolamine to phosphatidylserine in Chinese hamster ovary cells", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9642289/" } ], "x": 2938.666666666667, "y": 1764.8013698630136, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483096", "label": "PETA is dephosphorylated to ETA by PHOSPHO1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16090" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483096", "summary": "PETA is dephosphorylated to ETA by PHOSPHO1 is a Reactome release 97 curated reaction event. The source record connects PETA, H2O to ETA, Pi in cytosol and cross-references RHEA:16090.", "equation": "PETA + H2O → ETA + Pi", "inputs": [ { "node_id": "R-ALL-428686", "reactome_id": "R-ALL-428686", "label": "PETA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17553", "formula": "C2H8NO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498751", "reactome_id": "R-ALL-1498751", "label": "ETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57603", "formula": "C2H8NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphoethanolamine phosphatase activity of PHOSPHO1:Mg2+ [cytosol]", "activity_id": "2267363", "ec_numbers": [ "3.1.3.75" ], "accessions": [] } ], "citations": [ { "id": "PMID:15175005", "title": "Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15175005/" } ], "x": 2938.666666666667, "y": 1562.3082191780823, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483099", "label": "PA translocates from the outer to the inner mitochondrial membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial outer membrane · mitochondrial inner membrane", "compartment_ids": [ "GO:0005741", "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483099", "summary": "PA translocates from the outer to the inner mitochondrial membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PA to PA in mitochondrial outer membrane · mitochondrial inner membrane.", "equation": "PA → PA", "inputs": [ { "node_id": "R-ALL-1500590", "reactome_id": "R-ALL-1500590", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524101", "reactome_id": "R-ALL-1524101", "label": "PA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:2344448", "title": "Transport of phosphatidic acid within the mitochondrion", "journal": "Biochim Biophys Acta", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2344448/" }, { "id": "PMID:10514455", "title": "Phosphatidic acid synthesis in mitochondria. Topography of formation and transmembrane migration", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10514455/" } ], "x": 2938.666666666667, "y": 1892.6917808219177, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1483107", "label": "GPETA is hydrolyzed to ETA and G3P by GPCPD1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483107", "summary": "GPETA is hydrolyzed to ETA and G3P by GPCPD1 is a Reactome release 97 curated reaction event. The source record connects GPETA, H2O to ETA, G3P in cytosol.", "equation": "GPETA + H2O → ETA + G3P", "inputs": [ { "node_id": "R-ALL-1524023", "reactome_id": "R-ALL-1524023", "label": "GPETA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16929", "formula": "C5H14NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498751", "reactome_id": "R-ALL-1498751", "label": "ETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57603", "formula": "C2H8NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76114", "reactome_id": "R-ALL-76114", "label": "G3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophosphodiester phosphodiesterase activity of GPCPD1 [cytosol]", "activity_id": "1524092", "ec_numbers": [ "3.1.4.46" ], "accessions": [ "Q9NPB8" ] } ], "citations": [ { "id": "PMID:14702039", "title": "Complete sequencing and characterization of 21,243 full-length human cDNAs", "journal": "Nat Genet", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14702039/" } ], "x": 2381.3333333333335, "y": 1770.1301369863013, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483116", "label": "GPCho is hydrolyzed to Cho and G3P by GPCPD1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16062" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483116", "summary": "GPCho is hydrolyzed to Cho and G3P by GPCPD1 is a Reactome release 97 curated reaction event. The source record connects GPCho, H2O to Cho, G3P in cytosol and cross-references RHEA:16062.", "equation": "GPCho + H2O → Cho + G3P", "inputs": [ { "node_id": "R-ALL-1498750", "reactome_id": "R-ALL-1498750", "label": "GPCho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16870", "formula": "C8H20NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-264618", "reactome_id": "R-ALL-264618", "label": "Cho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76114", "reactome_id": "R-ALL-76114", "label": "G3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophosphodiester phosphodiesterase activity of GPCPD1 [cytosol]", "activity_id": "1524092", "ec_numbers": [ "3.1.4.46" ], "accessions": [ "Q9NPB8" ] } ], "citations": [ { "id": "PMID:14702039", "title": "Complete sequencing and characterization of 21,243 full-length human cDNAs", "journal": "Nat Genet", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14702039/" } ], "x": 2381.3333333333335, "y": 1567.63698630137, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483121", "label": "PA is converted to CDP-DAG by CDS1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483121", "summary": "PA is converted to CDP-DAG by CDS1 is a Reactome release 97 curated reaction event. The source record connects PA, CTP to CDP-DAG, PPi in endoplasmic reticulum membrane · cytosol.", "equation": "PA + CTP → CDP-DAG + PPi", "inputs": [ { "node_id": "R-ALL-426755", "reactome_id": "R-ALL-426755", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-110614", "reactome_id": "R-ALL-110614", "label": "CTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37563", "formula": "C9H12N3O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426955", "reactome_id": "R-ALL-426955", "label": "CDP-DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58332", "formula": "C14H17N3O15P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidate cytidylyltransferase activity of CDS1:Mg2+ [endoplasmic reticulum membrane]", "activity_id": "1524098", "ec_numbers": [ "2.7.7.41" ], "accessions": [] } ], "citations": [ { "id": "PMID:9407135", "title": "The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9407135/" }, { "id": "PMID:3718705", "title": "Participation of the microsomal CDP-diglycerides in the mitochondrial biosynthesis of phosphatidylglycerol", "journal": "Biochem Cell Biol", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3718705/" } ], "x": 2660, "y": 1770.1301369863013, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483142", "label": "PC is transphosphatidylated to PG by PLD1-4/6", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483142", "summary": "PC is transphosphatidylated to PG by PLD1-4/6 is a Reactome release 97 curated reaction event. The source record connects PC, Glycerol to PG, Cho in endoplasmic reticulum membrane · cytosol.", "equation": "PC + Glycerol → PG + Cho", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76116", "reactome_id": "R-ALL-76116", "label": "Glycerol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17754", "formula": "C3H8O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1483256", "reactome_id": "R-ALL-1483256", "label": "PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-264618", "reactome_id": "R-ALL-264618", "label": "Cho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "D-type glycerophospholipase activity of PLD1-4/6 [endoplasmic reticulum membrane]", "activity_id": "1524118", "ec_numbers": [ "3.1.4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:8530346", "title": "Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8530346/" }, { "id": "PMID:9761774", "title": "Characterization of human PLD2 and the analysis of PLD isoform splice variants", "journal": "FASEB J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9761774/" }, { "id": "PMID:9140189", "title": "A human homolog of the vaccinia virus HindIII K4L gene is a member of the phospholipase D superfamily", "journal": "Virus Res", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9140189/" } ], "x": 2938.666666666667, "y": 1770.1301369863013, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483159", "label": "PCho is dephosphorylated to Cho by PHOSPHO1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483159", "summary": "PCho is dephosphorylated to Cho by PHOSPHO1 is a Reactome release 97 curated reaction event. The source record connects PCho, H2O to Cho, Pi in cytosol.", "equation": "PCho + H2O → Cho + Pi", "inputs": [ { "node_id": "R-ALL-1524071", "reactome_id": "R-ALL-1524071", "label": "PCho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36700", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-264618", "reactome_id": "R-ALL-264618", "label": "Cho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphocholine phosphatase activity of PHOSPHO1:Mg2+ [cytosol]", "activity_id": "1500635", "ec_numbers": [ "3.1.3.75" ], "accessions": [] } ], "citations": [ { "id": "PMID:15175005", "title": "Human PHOSPHO1 exhibits high specific phosphoethanolamine and phosphocholine phosphatase activities", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15175005/" } ], "x": 2381.3333333333335, "y": 1775.4589041095892, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483165", "label": "PA is converted to CDP-DAG by CDS2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483165", "summary": "PA is converted to CDP-DAG by CDS2 is a Reactome release 97 curated reaction event. The source record connects PA, CTP to CDP-DAG, PPi in mitochondrial inner membrane · mitochondrial matrix.", "equation": "PA + CTP → CDP-DAG + PPi", "inputs": [ { "node_id": "R-ALL-1524101", "reactome_id": "R-ALL-1524101", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-110577", "reactome_id": "R-ALL-110577", "label": "CTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37563", "formula": "C9H12N3O14P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524064", "reactome_id": "R-ALL-1524064", "label": "CDP-DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58332", "formula": "C14H17N3O15P2R2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidate cytidylyltransferase activity of CDS2 [mitochondrial inner membrane]", "activity_id": "1524097", "ec_numbers": [ "2.7.7.41" ], "accessions": [ "O95674" ] } ], "citations": [ { "id": "PMID:9407135", "title": "The role of CDP-diacylglycerol synthetase and phosphatidylinositol synthase activity levels in the regulation of cellular phosphatidylinositol content", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9407135/" }, { "id": "PMID:8380172", "title": "Cardiolipin is synthesized on the matrix side of the inner membrane in rat liver mitochondria", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8380172/" }, { "id": "PMID:3718705", "title": "Participation of the microsomal CDP-diglycerides in the mitochondrial biosynthesis of phosphatidylglycerol", "journal": "Biochem Cell Biol", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3718705/" } ], "x": 2660, "y": 1775.4589041095892, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483170", "label": "PS transports from the ER membrane to the IM membrane", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · mitochondrial inner membrane", "compartment_ids": [ "GO:0005789", "GO:0005743" ], "rhea_ids": [ "RHEA:38663" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483170", "summary": "PS transports from the ER membrane to the IM membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PS to PS in endoplasmic reticulum membrane · mitochondrial inner membrane and cross-references RHEA:38663.", "equation": "PS → PS", "inputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9025386", "reactome_id": "R-ALL-9025386", "label": "PS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:2332429", "title": "Phospholipid synthesis in a membrane fraction associated with mitochondria", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2332429/" }, { "id": "PMID:1898727", "title": "Newly made phosphatidylserine and phosphatidylethanolamine are preferentially translocated between rat liver mitochondria and endoplasmic reticulum", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1898727/" } ], "x": 2938.666666666667, "y": 1668.8835616438357, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1483174", "label": "PE is methylated to PC by PEMT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483174", "summary": "PE is methylated to PC by PEMT is a Reactome release 97 curated reaction event. The source record connects PE, AdoMet to PC, AdoHcy in endoplasmic reticulum membrane · cytosol.", "equation": "PE + 3 AdoMet → PC + 3 AdoHcy", "inputs": [ { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylethanolamine N-methyltransferase activity of PEMT [endoplasmic reticulum membrane]", "activity_id": "1524127", "ec_numbers": [ "2.1.1.17" ], "accessions": [ "Q9UBM1" ] } ], "citations": [ { "id": "PMID:10100195", "title": "Activity of phosphatidylethanolamine-N-methyltransferase in brain affected by Alzheimer's disease", "journal": "Neurochem Int", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10100195/" }, { "id": "PMID:3057511", "title": "The methylation of phosphatidylethanolamine", "journal": "Prog Lipid Res", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3057511/" }, { "id": "PMID:11420179", "title": "Structure, expression profile and alternative processing of the human phosphatidylethanolamine N-methyltransferase (PEMT) gene", "journal": "Biochim Biophys Acta", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11420179/" } ], "x": 2938.666666666667, "y": 1775.4589041095892, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483182", "label": "PC is hydrolyzed to PA and choline by PLD1/2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483182", "summary": "PC is hydrolyzed to PA and choline by PLD1/2 is a Reactome release 97 curated reaction event. The source record connects PC, H2O to PA, Cho in endoplasmic reticulum membrane · cytosol.", "equation": "PC + H2O → PA + Cho", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426755", "reactome_id": "R-ALL-426755", "label": "PA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-264618", "reactome_id": "R-ALL-264618", "label": "Cho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "D-type glycerophospholipase activity of PLD1/2 [endoplasmic reticulum membrane]", "activity_id": "1500629", "ec_numbers": [ "3.1.4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:8530346", "title": "Human ADP-ribosylation factor-activated phosphatidylcholine-specific phospholipase D defines a new and highly conserved gene family", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8530346/" }, { "id": "PMID:9582313", "title": "Cloning and initial characterization of a human phospholipase D2 (hPLD2). ADP-ribosylation factor regulates hPLD2", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9582313/" } ], "x": 2381.3333333333335, "y": 1780.7876712328766, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483186", "label": "PC is converted to PS by PTDSS1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:45088" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483186", "summary": "PC is converted to PS by PTDSS1 is a Reactome release 97 curated reaction event. The source record connects PC, L-Ser to PS, Cho in endoplasmic reticulum membrane · cytosol and cross-references RHEA:45088.", "equation": "PC + L-Ser → PS + Cho", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-264618", "reactome_id": "R-ALL-264618", "label": "Cho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transferase activity of PTDSS1 [endoplasmic reticulum membrane]", "activity_id": "1524119", "ec_numbers": [ "2" ], "accessions": [ "P48651" ] } ], "citations": [ { "id": "PMID:19014349", "title": "Purification and characterization of human phosphatidylserine synthases 1 and 2", "journal": "Biochem J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19014349/" }, { "id": "PMID:9642289", "title": "Genetic evidence that phosphatidylserine synthase II catalyzes the conversion of phosphatidylethanolamine to phosphatidylserine in Chinese hamster ovary cells", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9642289/" } ], "x": 2660, "y": 1567.63698630137, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483190", "label": "PETA and CTP are condensed to CDP-ETA by PCY2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:24593" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483190", "summary": "PETA and CTP are condensed to CDP-ETA by PCY2 is a Reactome release 97 curated reaction event. The source record connects PETA, CTP to CDP-ETA, PPi in endoplasmic reticulum membrane · cytosol and cross-references RHEA:24593.", "equation": "PETA + CTP → CDP-ETA + PPi", "inputs": [ { "node_id": "R-ALL-428686", "reactome_id": "R-ALL-428686", "label": "PETA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17553", "formula": "C2H8NO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-110614", "reactome_id": "R-ALL-110614", "label": "CTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37563", "formula": "C9H12N3O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500603", "reactome_id": "R-ALL-1500603", "label": "CDP-ETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57876", "formula": "C11H19N4O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ethanolamine-phosphate cytidylyltransferase activity of PCYT2 dimer [endoplasmic reticulum membrane]", "activity_id": "1500628", "ec_numbers": [ "2.7.7.14" ], "accessions": [] } ], "citations": [ { "id": "PMID:9083101", "title": "Cloning of a human cDNA for CTP-phosphoethanolamine cytidylyltransferase by complementation in vivo of a yeast mutant", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9083101/" }, { "id": "PMID:18583706", "title": "Stimulation of the human CTP:phosphoethanolamine cytidylyltransferase gene by early growth response protein 1", "journal": "J Lipid Res", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18583706/" } ], "x": 2938.666666666667, "y": 1567.63698630137, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483197", "label": "PTPMT1 dephosphorylates PGP to PG", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:33751" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483197", "summary": "PTPMT1 dephosphorylates PGP to PG is a Reactome release 97 curated reaction event. The source record connects H2O, PGP to PG, Pi in mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:33751.", "equation": "H2O + PGP → PG + Pi", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1524059", "reactome_id": "R-ALL-1524059", "label": "PGP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60110", "formula": "C8H11O13P2R2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524042", "reactome_id": "R-ALL-1524042", "label": "PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylglycerophosphatase activity of PTPMT1 [mitochondrial inner membrane]", "activity_id": "3446787", "ec_numbers": [ "3.1.3.27" ], "accessions": [ "Q8WUK0" ] } ], "citations": [ { "id": "PMID:21641550", "title": "Mitochondrial phosphatase PTPMT1 is essential for cardiolipin biosynthesis", "journal": "Cell Metab.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21641550/" } ], "x": 2381.3333333333335, "y": 1572.9657534246576, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483203", "label": "PA is dephosphorylated to DAG by LPIN", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483203", "summary": "PA is dephosphorylated to DAG by LPIN is a Reactome release 97 curated reaction event. The source record connects PA, H2O to DAG, Pi in endoplasmic reticulum membrane · cytosol.", "equation": "PA + H2O → DAG + Pi", "inputs": [ { "node_id": "R-ALL-426755", "reactome_id": "R-ALL-426755", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidate phosphatase activity of LPIN [endoplasmic reticulum membrane]", "activity_id": "1500627", "ec_numbers": [ "3.1.3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:17158099", "title": "Three mammalian lipins act as phosphatidate phosphatases with distinct tissue expression patterns", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17158099/" }, { "id": "PMID:18694939", "title": "Temporal and spatial regulation of the phosphatidate phosphatases lipin 1 and 2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18694939/" } ], "x": 2660, "y": 1780.7876712328766, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483209", "label": "PG is converted to BMP", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane", "compartment_ids": [ "GO:0031902" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483209", "summary": "PG is converted to BMP is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PG to BMP in late endosome membrane.", "equation": "PG → BMP", "inputs": [ { "node_id": "R-ALL-1524139", "reactome_id": "R-ALL-1524139", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1498732", "reactome_id": "R-ALL-1498732", "label": "BMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60815", "formula": "C10H23O8P", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10101262", "title": "Transacylase formation of bis(monoacylglycerol)phosphate", "journal": "Biochim Biophys Acta", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10101262/" }, { "id": "PMID:21136156", "title": "Biological Function of the Cellular Lipid BMP-BMP as a Key Activator for Cholesterol Sorting and Membrane Digestion", "journal": "Neurochem Res", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21136156/" }, { "id": "PMID:650089", "title": "Conversion of diphosphatidylglycerol to bis(monoacylglyceryl)phosphate by lysosomes", "journal": "J Lipid Res", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/650089/" }, { "id": "PMID:17558022", "title": "De novo biosynthesis of the late endosome lipid, bis(monoacylglycero)phosphate", "journal": "J Lipid Res", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17558022/" } ], "x": 2938.666666666667, "y": 1903.349315068493, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-1483211", "label": "PC:PITPNB is transported from the Golgi membrane to the ER membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0000139", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483211", "summary": "PC:PITPNB is transported from the Golgi membrane to the ER membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PC:PITPNB to PC:PITPNB in Golgi membrane · endoplasmic reticulum membrane.", "equation": "PC:PITPNB → PC:PITPNB", "inputs": [ { "node_id": "R-HSA-1524122", "reactome_id": "R-HSA-1524122", "label": "PC:PITPNB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1524110", "reactome_id": "R-HSA-1524110", "label": "PC:PITPNB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine transfer activity of PC:PITPNB [Golgi membrane]", "activity_id": "9855943", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18636990", "title": "Dynamics of lipid transfer by phosphatidylinositol transfer proteins in cells", "journal": "Traffic", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18636990/" }, { "id": "PMID:20332109", "title": "Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum", "journal": "J Cell Sci", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20332109/" }, { "id": "PMID:11104777", "title": "Structure of a multifunctional protein. Mammalian phosphatidylinositol transfer protein complexed with phosphatidylcholine", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11104777/" } ], "x": 2381.3333333333335, "y": 1876.7054794520548, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1483212", "label": "PS is decarboxylated to PE by PISD", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483212", "summary": "PS is decarboxylated to PE by PISD is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PS to PE, CO2 in mitochondrial inner membrane · mitochondrial matrix.", "equation": "PS → PE + CO2", "inputs": [ { "node_id": "R-ALL-9025386", "reactome_id": "R-ALL-9025386", "label": "PS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524083", "reactome_id": "R-ALL-1524083", "label": "PE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylserine decarboxylase activity of PISD:Pyruvoyl [mitochondrial inner membrane]", "activity_id": "1524158", "ec_numbers": [ "4.1.1.65" ], "accessions": [] } ], "citations": [ { "id": "PMID:17478478", "title": "High-throughput mass spectrometry screening for inhibitors of phosphatidylserine decarboxylase", "journal": "J Biomol Screen", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17478478/" } ], "x": 2660, "y": 1850.0616438356165, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1483218", "label": "PG transports from the ER membrane to the late endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · late endosome membrane", "compartment_ids": [ "GO:0005789", "GO:0031902" ], "rhea_ids": [ "RHEA:39743" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483218", "summary": "PG transports from the ER membrane to the late endosome membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PG to PG in endoplasmic reticulum membrane · late endosome membrane and cross-references RHEA:39743.", "equation": "PG → PG", "inputs": [ { "node_id": "R-ALL-1483256", "reactome_id": "R-ALL-1483256", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524139", "reactome_id": "R-ALL-1524139", "label": "PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:17558022", "title": "De novo biosynthesis of the late endosome lipid, bis(monoacylglycero)phosphate", "journal": "J Lipid Res", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17558022/" }, { "id": "PMID:9515966", "title": "A lipid associated with the antiphospholipid syndrome regulates endosome structure and function", "journal": "Nature", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9515966/" }, { "id": "PMID:10559883", "title": "Late endosomal membranes rich in lysobisphosphatidic acid regulate cholesterol transport", "journal": "Nat Cell Biol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10559883/" }, { "id": "PMID:15350976", "title": "Short-range intracellular trafficking of small molecules across endoplasmic reticulum junctions", "journal": "Trends Cell Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15350976/" }, { "id": "PMID:20118922", "title": "Membrane contacts between endosomes and ER provide sites for PTP1B-epidermal growth factor receptor interaction", "journal": "Nat Cell Biol", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20118922/" } ], "x": 2381.3333333333335, "y": 1668.8835616438357, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1483219", "label": "PC is exchanged with PI by PITPNB", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483219", "summary": "PC is exchanged with PI by PITPNB is a Reactome release 97 curated reaction event. The source record connects PC:PITPNB, PI to PI:PITPNB, PC in endoplasmic reticulum membrane.", "equation": "PC:PITPNB + PI → PI:PITPNB + PC", "inputs": [ { "node_id": "R-HSA-1524110", "reactome_id": "R-HSA-1524110", "label": "PC:PITPNB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1524117", "reactome_id": "R-HSA-1524117", "label": "PI:PITPNB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine transfer activity of PC:PITPNB [endoplasmic reticulum membrane]", "activity_id": "9855941", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18636990", "title": "Dynamics of lipid transfer by phosphatidylinositol transfer proteins in cells", "journal": "Traffic", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18636990/" }, { "id": "PMID:20332109", "title": "Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum", "journal": "J Cell Sci", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20332109/" }, { "id": "PMID:11104777", "title": "Structure of a multifunctional protein. Mammalian phosphatidylinositol transfer protein complexed with phosphatidylcholine", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11104777/" }, { "id": "PMID:16274224", "title": "Structure of PITPbeta in complex with phosphatidylcholine: comparison of structure and lipid transfer to other PITP isoforms", "journal": "Biochemistry", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16274224/" }, { "id": "PMID:11980708", "title": "Structure of apo-phosphatidylinositol transfer protein alpha provides insight into membrane association", "journal": "EMBO J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980708/" }, { "id": "PMID:14962392", "title": "Structure-function analysis of human [corrected] phosphatidylinositol transfer protein alpha bound to phosphatidylinositol", "journal": "Structure", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14962392/" } ], "x": 2381.3333333333335, "y": 1855.390410958904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483222", "label": "ETA is phosphorylated to PETA by CHK/ETNK", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13070" ], "source_url": "https://reactome.org/content/detail/R-HSA-1483222", "summary": "ETA is phosphorylated to PETA by CHK/ETNK is a Reactome release 97 curated reaction event. The source record connects ETA, ATP to PETA, ADP in cytosol and cross-references RHEA:13070.", "equation": "ETA + ATP → PETA + ADP", "inputs": [ { "node_id": "R-ALL-1498751", "reactome_id": "R-ALL-1498751", "label": "ETA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57603", "formula": "C2H8NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-428686", "reactome_id": "R-ALL-428686", "label": "PETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17553", "formula": "C2H8NO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ethanolamine kinase activity of CHK/ETNK [cytosol]", "activity_id": "1500609", "ec_numbers": [ "2.7.1.82" ], "accessions": [] } ], "citations": [ { "id": "PMID:19915674", "title": "Differential role of human choline kinase alpha and beta enzymes in lipid metabolism: implications in cancer onset and treatment", "journal": "PLoS One", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19915674/" }, { "id": "PMID:11044454", "title": "Overexpression of a mammalian ethanolamine-specific kinase accelerates the CDP-ethanolamine pathway", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11044454/" } ], "x": 2660, "y": 1572.9657534246576, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1483229", "label": "PI:PITPNB is transported from the ER membrane to the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · Golgi membrane", "compartment_ids": [ "GO:0005789", "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1483229", "summary": "PI:PITPNB is transported from the ER membrane to the Golgi membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PI:PITPNB to PI:PITPNB in endoplasmic reticulum membrane · Golgi membrane.", "equation": "PI:PITPNB → PI:PITPNB", "inputs": [ { "node_id": "R-HSA-1524117", "reactome_id": "R-HSA-1524117", "label": "PI:PITPNB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1524150", "reactome_id": "R-HSA-1524150", "label": "PI:PITPNB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol transfer activity of PI:PITPNB [endoplasmic reticulum membrane]", "activity_id": "9855944", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18636990", "title": "Dynamics of lipid transfer by phosphatidylinositol transfer proteins in cells", "journal": "Traffic", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18636990/" }, { "id": "PMID:20332109", "title": "Phosphatidylinositol- and phosphatidylcholine-transfer activity of PITPbeta is essential for COPI-mediated retrograde transport from the Golgi to the endoplasmic reticulum", "journal": "J Cell Sci", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20332109/" }, { "id": "PMID:11104777", "title": "Structure of a multifunctional protein. Mammalian phosphatidylinositol transfer protein complexed with phosphatidylcholine", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11104777/" } ], "x": 2660, "y": 1876.7054794520548, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1497784", "label": "The cofactor BH4 is required for electron transfer in the eNOS catalytic cycle", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497784", "summary": "The cofactor BH4 is required for electron transfer in the eNOS catalytic cycle is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects p-S1177-eNOS:CaM:HSP90:p-AKT1, BH4 to p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4 in cytosol · plasma membrane.", "equation": "p-S1177-eNOS:CaM:HSP90:p-AKT1 + BH4 → p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4", "inputs": [ { "node_id": "R-HSA-202121", "reactome_id": "R-HSA-202121", "label": "p-S1177-eNOS:CaM:HSP90:p-AKT1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1497830", "reactome_id": "R-HSA-1497830", "label": "p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "tetrahydrobiopterin binding activity of p-S1177-eNOS:CaM:HSP90:p-AKT1 [plasma membrane]", "activity_id": "9911133", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15476407", "title": "Redox function of tetrahydrobiopterin and effect of L-arginine on oxygen binding in endothelial nitric oxide synthase", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15476407/" }, { "id": "PMID:19583767", "title": "Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain", "journal": "FEBS J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19583767/" } ], "x": 4730, "y": 3589.9583333333335, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1497794", "label": "Salvage - BH2 is reduced to BH4 by DHFR", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497794", "summary": "Salvage - BH2 is reduced to BH4 by DHFR is a Reactome release 97 curated reaction event. The source record connects q-BH2, H+, NADPH to BH4, NADP+ in cytosol.", "equation": "q-BH2 + H+ + NADPH → BH4 + NADP+", "inputs": [ { "node_id": "R-ALL-1497857", "reactome_id": "R-ALL-1497857", "label": "q-BH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43029", "formula": "C9H13N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dihydrofolate reductase activity of DHFR dimer [cytosol]", "activity_id": "1497819", "ec_numbers": [ "1.5.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:19666465", "title": "Critical role for tetrahydrobiopterin recycling by dihydrofolate reductase in regulation of endothelial nitric-oxide synthase coupling: relative importance of the de novo biopterin synthesis versus salvage pathways", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19666465/" } ], "x": 4451.333333333333, "y": 3460.2916666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1497796", "label": "BH2 binding can lead to eNOS uncoupling", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497796", "summary": "BH2 binding can lead to eNOS uncoupling is a Reactome release 97 curated reaction event. The source record connects p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4, q-BH2 to p-S1177-eNOS:CaM:HSP90:p-AKT1:BH2, BH4 in cytosol.", "equation": "p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4 + q-BH2 → p-S1177-eNOS:CaM:HSP90:p-AKT1:BH2 + BH4", "inputs": [ { "node_id": "R-HSA-1497830", "reactome_id": "R-HSA-1497830", "label": "p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-1497857", "reactome_id": "R-ALL-1497857", "label": "q-BH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43029", "formula": "C9H13N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1497889", "reactome_id": "R-HSA-1497889", "label": "p-S1177-eNOS:CaM:HSP90:p-AKT1:BH2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "tetrahydrobiopterin binding activity of p-S1177-eNOS:CaM:HSP90:p-AKT1:BH4 [plasma membrane]", "activity_id": "9911168", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11879202", "title": "The ratio between tetrahydrobiopterin and oxidized tetrahydrobiopterin analogues controls superoxide release from endothelial nitric oxide synthase: an EPR spin trapping study", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11879202/" } ], "x": 5008.666666666667, "y": 3492.7083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1497824", "label": "BH4 is oxidised to the BH3 radical during the eNOS catalytic cycle", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497824", "summary": "BH4 is oxidised to the BH3 radical during the eNOS catalytic cycle is a Reactome release 97 curated reaction event. The source record connects BH4 to BH3., e- in cytosol.", "equation": "BH4 → BH3. + e-", "inputs": [ { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1497797", "reactome_id": "R-ALL-1497797", "label": "BH3.", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62772", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-879326", "reactome_id": "R-ALL-879326", "label": "e-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:10545", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15476407", "title": "Redox function of tetrahydrobiopterin and effect of L-arginine on oxygen binding in endothelial nitric oxide synthase", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15476407/" } ], "x": 5008.666666666667, "y": 3589.9583333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1497853", "label": "Sepiapterin reductase (SPR) is phosphorylated by Ca2+/calmodulin-dependent protein kinase II", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497853", "summary": "Sepiapterin reductase (SPR) is phosphorylated by Ca2+/calmodulin-dependent protein kinase II is a Reactome release 97 curated reaction event. The source record connects SPR dimer, ATP to ADP, p-SPR dimer in cytosol.", "equation": "SPR dimer + 2 ATP → 2 ADP + p-SPR dimer", "inputs": [ { "node_id": "R-HSA-1497791", "reactome_id": "R-HSA-1497791", "label": "SPR dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-1497817", "reactome_id": "R-HSA-1497817", "label": "p-SPR dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cGMP-dependent protein kinase activity of PRKG2 [cytosol]", "activity_id": "1475413", "ec_numbers": [ "2.7.11.12" ], "accessions": [ "Q13237" ] } ], "citations": [ { "id": "PMID:8137944", "title": "Phosphorylation by Ca2+/calmodulin-dependent protein kinase II and protein kinase C of sepiapterin reductase, the terminal enzyme in the biosynthetic pathway of tetrahydrobiopterin", "journal": "FEBS Lett", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8137944/" }, { "id": "PMID:11825621", "title": "Mutational analysis of sites in sepiapterin reductase phosphorylated by Ca2+/calmodulin-dependent protein kinase II", "journal": "Biochim Biophys Acta", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11825621/" } ], "x": 4451.333333333333, "y": 3525.125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1497855", "label": "Ascorbate can reduce the BH3 radical back to BH4", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497855", "summary": "Ascorbate can reduce the BH3 radical back to BH4 is a Reactome release 97 curated reaction event. The source record connects BH3., AscH- to BH4, Asc.- in cytosol.", "equation": "BH3. + AscH- → BH4 + Asc.-", "inputs": [ { "node_id": "R-ALL-1497797", "reactome_id": "R-ALL-1497797", "label": "BH3.", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62772", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-198816", "reactome_id": "R-ALL-198816", "label": "AscH-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:38290", "formula": "C6H7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1497805", "reactome_id": "R-ALL-1497805", "label": "Asc.-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59513", "formula": "C6H6O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11243424", "title": "Effect of vitamin C on the availability of tetrahydrobiopterin in human endothelial cells", "journal": "J Cardiovasc Pharmacol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11243424/" }, { "id": "PMID:12692136", "title": "Interactions of peroxynitrite, tetrahydrobiopterin, ascorbic acid, and thiols: implications for uncoupling endothelial nitric-oxide synthase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12692136/" }, { "id": "PMID:11827745", "title": "Oxidation of tetrahydrobiopterin by biological radicals and scavenging of the trihydrobiopterin radical by ascorbate", "journal": "Free Radic Biol Med", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11827745/" } ], "x": 5008.666666666667, "y": 3557.5416666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1497863", "label": "The BH3 radical can decay to dihydrobiopterin (BH2)", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497863", "summary": "The BH3 radical can decay to dihydrobiopterin (BH2) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects BH3. to q-BH2 in cytosol.", "equation": "BH3. → q-BH2", "inputs": [ { "node_id": "R-ALL-1497797", "reactome_id": "R-ALL-1497797", "label": "BH3.", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62772", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1497857", "reactome_id": "R-ALL-1497857", "label": "q-BH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43029", "formula": "C9H13N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10512739", "title": "Oxidation of tetrahydrobiopterin by peroxynitrite: implications for vascular endothelial function", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10512739/" } ], "x": 4451.333333333333, "y": 3654.7916666666665, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-1497866", "label": "Peroxynitrite can oxidise BH4 to the BH3 radical", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497866", "summary": "Peroxynitrite can oxidise BH4 to the BH3 radical is a Reactome release 97 curated reaction event. The source record connects BH4, Peroxynitrite to BH3. in cytosol.", "equation": "BH4 + Peroxynitrite → BH3.", "inputs": [ { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1222481", "reactome_id": "R-ALL-1222481", "label": "Peroxynitrite", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:25941", "formula": "NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1497797", "reactome_id": "R-ALL-1497797", "label": "BH3.", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62772", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12692136", "title": "Interactions of peroxynitrite, tetrahydrobiopterin, ascorbic acid, and thiols: implications for uncoupling endothelial nitric-oxide synthase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12692136/" } ], "x": 4451.333333333333, "y": 3622.375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1497869", "label": "Salvage - Sepiapterin is reduced to q-BH2", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18955" ], "source_url": "https://reactome.org/content/detail/R-HSA-1497869", "summary": "Salvage - Sepiapterin is reduced to q-BH2 is a Reactome release 97 curated reaction event. The source record connects NADPH, sepiapterin, H+ to q-BH2, NADP+ in cytosol and cross-references RHEA:18955.", "equation": "NADPH + sepiapterin + H+ → q-BH2 + NADP+", "inputs": [ { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1497811", "reactome_id": "R-ALL-1497811", "label": "sepiapterin", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:194527", "formula": "C9H11N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1497857", "reactome_id": "R-ALL-1497857", "label": "q-BH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43029", "formula": "C9H13N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sepiapterin reductase (NADP+) activity of p-SPR dimer [cytosol]", "activity_id": "1497839", "ec_numbers": [ "1.1.1.153" ], "accessions": [] } ], "citations": [ { "id": "PMID:18511317", "title": "Cellular uptake of sepiapterin and push-pull accumulation of tetrahydrobiopterin", "journal": "Mol Genet Metab", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18511317/" } ], "x": 4451.333333333333, "y": 3395.4583333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1497883", "label": "Ferrous iron reduces the BH3 radical back to BH4", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1497883", "summary": "Ferrous iron reduces the BH3 radical back to BH4 is a Reactome release 97 curated reaction event. The source record connects Fe2+, BH3. to BH4, Fe3+ in cytosol.", "equation": "Fe2+ + BH3. → BH4 + Fe3+", "inputs": [ { "node_id": "R-ALL-71067", "reactome_id": "R-ALL-71067", "label": "Fe2+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1497797", "reactome_id": "R-ALL-1497797", "label": "BH3.", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62772", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111736", "reactome_id": "R-ALL-111736", "label": "Fe3+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29034", "formula": "Fe", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15476407", "title": "Redox function of tetrahydrobiopterin and effect of L-arginine on oxygen binding in endothelial nitric oxide synthase", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15476407/" } ], "x": 4451.333333333333, "y": 3589.9583333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-156526", "label": "CYP1A2,3A4,3A5,2A13 oxidise AFB1 to AFXBO", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-156526", "summary": "CYP1A2,3A4,3A5,2A13 oxidise AFB1 to AFXBO is a Reactome release 97 curated reaction event. The source record connects AFB1, H+, NADPH to AFXBO, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "AFB1 + H+ + NADPH + O2 → AFXBO + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-5423605", "reactome_id": "R-ALL-5423605", "label": "AFB1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2504", "formula": "C17H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159211", "reactome_id": "R-ALL-159211", "label": "AFXBO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30725", "formula": "C17H12O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A2,3A4,3A5,2A13 [endoplasmic reticulum membrane]", "activity_id": "5423603", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8975785", "title": "The kinetics of aflatoxin B1 oxidation by human cDNA-expressed and human liver microsomal cytochromes P450 1A2 and 3A4", "journal": "Toxicol. Appl. Pharmacol.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8975785/" }, { "id": "PMID:16385575", "title": "Efficient activation of aflatoxin B1 by cytochrome P450 2A13, an enzyme predominantly expressed in human respiratory tract", "journal": "Int. J. Cancer", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16385575/" } ], "x": 7740, "y": 2951.735294117647, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-158468", "label": "SULT1A1 dimer sulfonates PARA to PARA-SO4", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-158468", "summary": "SULT1A1 dimer sulfonates PARA to PARA-SO4 is a Reactome release 97 curated reaction event. The source record connects PAPS, APAP to PARA-SO4, PAP in cytosol.", "equation": "PAPS + APAP → PARA-SO4 + PAP", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9677362", "reactome_id": "R-ALL-9677362", "label": "APAP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158472", "reactome_id": "R-ALL-158472", "label": "PARA-SO4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32635", "formula": "C8H9NO5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aryl sulfotransferase activity of SULT1A1 dimer [cytosol]", "activity_id": "158475", "ec_numbers": [ "2.8.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10503886", "title": "Sulfotransferase catalyzing sulfation of heterocyclic amines", "journal": "Cancer Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10503886/" }, { "id": "PMID:12372849", "title": "Sulfonation and molecular action", "journal": "Endocr Rev", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12372849/" } ], "x": 7740, "y": 3111.9117647058824, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-158609", "label": "TPMT transfers CH3 from AdoMet to 6MP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-158609", "summary": "TPMT transfers CH3 from AdoMet to 6MP is a Reactome release 97 curated reaction event. The source record connects 6MP, AdoMet to 6MMP, AdoHcy, H+ in cytosol.", "equation": "6MP + AdoMet → 6MMP + AdoHcy + H+", "inputs": [ { "node_id": "R-ALL-158619", "reactome_id": "R-ALL-158619", "label": "6MP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2208", "formula": "C5H4N4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158611", "reactome_id": "R-ALL-158611", "label": "6MMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28279", "formula": "C6H6N4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "thiopurine S-methyltransferase activity of TPMT [cytosol]", "activity_id": "158629", "ec_numbers": [ "2.1.1.67" ], "accessions": [ "P51580" ] } ], "citations": [ { "id": "PMID:13981612", "title": "Metabolism of thiopyrimidines and thiopurines. S-Methylation with S-adenosylmethionine transmethylase and catabolism in mammalian tissues.", "journal": "J Biol Chem", "year": 1963, "url": "https://pubmed.ncbi.nlm.nih.gov/13981612/" }, { "id": "PMID:10331075", "title": "Methylation pharmacogenetics: catechol O-methyltransferase, thiopurine methyltransferase, and histamine N-methyltransferase", "journal": "Annu Rev Pharmacol Toxicol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10331075/" }, { "id": "PMID:16267626", "title": "The thiopurines: an update", "journal": "Invest New Drugs", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16267626/" }, { "id": "PMID:15788214", "title": "Thiopurines in inflammatory bowel disease: pharmacogenetics, therapeutic drug monitoring and clinical recommendations", "journal": "Dig Liver Dis", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15788214/" }, { "id": "PMID:24734162", "title": "Assessment of Thiopurine-based drugs according to Thiopurine S-methyltransferase genotype in patients with Acute Lymphoblastic Leukemia", "journal": "Iran J Ped Hematol Oncol", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24734162/" } ], "x": 7461.333333333333, "y": 3066.1470588235293, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-158832", "label": "The acetyl group from acetyl-CoA is transferred to NAT2", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-158832", "summary": "The acetyl group from acetyl-CoA is transferred to NAT2 is a Reactome release 97 curated reaction event. The source record connects NAT2, Ac-CoA to AcC-NAT2, CoA-SH in cytosol.", "equation": "NAT2 + Ac-CoA → AcC-NAT2 + CoA-SH", "inputs": [ { "node_id": "R-HSA-158692", "reactome_id": "R-HSA-158692", "label": "NAT2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-158833", "reactome_id": "R-HSA-158833", "label": "AcC-NAT2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein-cysteine S-acyltransferase activity of NAT2 [cytosol]", "activity_id": "9954139", "ec_numbers": [ "2.3.1" ], "accessions": [ "P11245" ] } ], "citations": [ { "id": "PMID:1559981", "title": "Site-directed mutagenesis of recombinant human arylamine N-acetyltransferase expressed in Escherichia coli. Evidence for direct involvement of Cys68 in the catalytic mechanism of polymorphic human NAT2.", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1559981/" } ], "x": 8018.666666666667, "y": 3119.5392156862745, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-158849", "label": "Phenol can form a sulfate conjugate", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-158849", "summary": "Phenol can form a sulfate conjugate is a Reactome release 97 curated reaction event. The source record connects PhOH, PAPS to Phenyl sulfate, PAP in cytosol.", "equation": "PhOH + PAPS → Phenyl sulfate + PAP", "inputs": [ { "node_id": "R-ALL-158858", "reactome_id": "R-ALL-158858", "label": "PhOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15882", "formula": "C6H6O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158850", "reactome_id": "R-ALL-158850", "label": "Phenyl sulfate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27905", "formula": "C6H6O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aryl sulfotransferase activity of SULT1A1 dimer [cytosol]", "activity_id": "158475", "ec_numbers": [ "2.8.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9034160", "title": "Sulfation and sulfotransferases 1: Sulfotransferase molecular biology: cDNAs and genes", "journal": "FASEB J", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9034160/" }, { "id": "PMID:12372849", "title": "Sulfonation and molecular action", "journal": "Endocr Rev", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12372849/" } ], "x": 7461.333333333333, "y": 3073.774509803922, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-158860", "label": "SULT1A1 dimer sulfonates NHABP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-158860", "summary": "SULT1A1 dimer sulfonates NHABP is a Reactome release 97 curated reaction event. The source record connects NHABP, PAPS to NHABP-SO4, PAP in cytosol.", "equation": "NHABP + PAPS → NHABP-SO4 + PAP", "inputs": [ { "node_id": "R-ALL-158847", "reactome_id": "R-ALL-158847", "label": "NHABP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16580", "formula": "C12H11NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158859", "reactome_id": "R-ALL-158859", "label": "NHABP-SO4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32701", "formula": "C12H11NO4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aryl sulfotransferase activity of SULT1A1 dimer [cytosol]", "activity_id": "158475", "ec_numbers": [ "2.8.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10503886", "title": "Sulfotransferase catalyzing sulfation of heterocyclic amines", "journal": "Cancer Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10503886/" }, { "id": "PMID:9034160", "title": "Sulfation and sulfotransferases 1: Sulfotransferase molecular biology: cDNAs and genes", "journal": "FASEB J", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9034160/" }, { "id": "PMID:12372849", "title": "Sulfonation and molecular action", "journal": "Endocr Rev", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12372849/" } ], "x": 7740, "y": 3073.774509803922, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159179", "label": "UGT1A4 transfers GlcA from UDP-GlcA to BMG to form BDG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two", "porphyrins" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-159179", "summary": "UGT1A4 transfers GlcA from UDP-GlcA to BMG to form BDG is a Reactome release 97 curated reaction event. The source record connects BMG, UDP-GlcA to BDG, UDP in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "BMG + UDP-GlcA → BDG + UDP", "inputs": [ { "node_id": "R-ALL-159160", "reactome_id": "R-ALL-159160", "label": "BMG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16427", "formula": "C39H44N4O12", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174384", "reactome_id": "R-ALL-174384", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159149", "reactome_id": "R-ALL-159149", "label": "BDG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58471", "formula": "C45H50N4O18", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158602", "reactome_id": "R-ALL-158602", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucuronosyltransferase activity of UGT1A4 [endoplasmic reticulum membrane]", "activity_id": "9704996", "ec_numbers": [ "2.4.1.17" ], "accessions": [ "P22310" ] } ], "citations": [ { "id": "PMID:1339448", "title": "A novel complex locus UGT1 encodes human bilirubin, phenol, and other UDP-glucuronosyltransferase isozymes with identical carboxyl termini", "journal": "J. Biol. Chem.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1339448/" } ], "x": 6521.333333333333, "y": 2983.6071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159194", "label": "UGT1A4 transfers GlcA from UDP-GlcA to BIL to form BMG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two", "porphyrins" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-159194", "summary": "UGT1A4 transfers GlcA from UDP-GlcA to BIL to form BMG is a Reactome release 97 curated reaction event. The source record connects BIL, UDP-GlcA to BMG, UDP in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "BIL + UDP-GlcA → BMG + UDP", "inputs": [ { "node_id": "R-ALL-159151", "reactome_id": "R-ALL-159151", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174384", "reactome_id": "R-ALL-174384", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159160", "reactome_id": "R-ALL-159160", "label": "BMG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16427", "formula": "C39H44N4O12", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158602", "reactome_id": "R-ALL-158602", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucuronosyltransferase activity of UGT1A4 [endoplasmic reticulum membrane]", "activity_id": "9704996", "ec_numbers": [ "2.4.1.17" ], "accessions": [ "P22310" ] } ], "citations": [ { "id": "PMID:8027054", "title": "Bilirubin UDP-glucuronosyltransferase 1 is the only relevant bilirubin glucuronidating isoform in man", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8027054/" }, { "id": "PMID:1339448", "title": "A novel complex locus UGT1 encodes human bilirubin, phenol, and other UDP-glucuronosyltransferase isozymes with identical carboxyl termini", "journal": "J. Biol. Chem.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1339448/" }, { "id": "PMID:6654901", "title": "The hepatic microsomal formation of bilirubin diglucuronide", "journal": "J Biol Chem", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6654901/" }, { "id": "PMID:6796486", "title": "Bilirubin mono- and diglucuronide formation by human liver in vitro: assay by high-pressure liquid chromatography", "journal": "Hepatology", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/6796486/" }, { "id": "PMID:3082969", "title": "Microsomal UDP-glucuronyltransferase-catalyzed bilirubin diglucuronide formation in human liver", "journal": "J Hepatol", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3082969/" }, { "id": "PMID:4639028", "title": "Bilirubin conjugates in bile of man and rat in the normal state and in liver disease", "journal": "J Clin Invest", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/4639028/" } ], "x": 6800, "y": 2983.6071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159358", "label": "SULT1A3,4 dimers sulfate DA to DAOS", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:67881" ], "source_url": "https://reactome.org/content/detail/R-HSA-159358", "summary": "SULT1A3,4 dimers sulfate DA to DAOS is a Reactome release 97 curated reaction event. The source record connects DA, PAPS to DAOS, PAP in cytosol and cross-references RHEA:67881.", "equation": "DA + PAPS → DAOS + PAP", "inputs": [ { "node_id": "R-ALL-159362", "reactome_id": "R-ALL-159362", "label": "DA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59905", "formula": "C8H12NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159352", "reactome_id": "R-ALL-159352", "label": "DAOS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:37946", "formula": "C8H11NO5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aryl sulfotransferase activity of SULT1A3,4 dimers [cytosol]", "activity_id": "159347", "ec_numbers": [ "2.8.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6139755", "title": "Thermolabile and thermostable human platelet phenol sulfotransferase. Substrate specificity and physical separation.", "journal": "Naunyn Schmiedebergs Arch Pharmacol", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6139755/" }, { "id": "PMID:6770210", "title": "Radioenzymatic assay of sulfate conjugates of catecholamines and DOPA in plasma", "journal": "Life Sci", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6770210/" }, { "id": "PMID:14622112", "title": "Human catecholamine sulfotransferase (SULT1A3) pharmacogenetics: functional genetic polymorphism", "journal": "J. Neurochem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14622112/" }, { "id": "PMID:15358107", "title": "Human SULT1A3 pharmacogenetics: gene duplication and functional genomic studies", "journal": "Biochem. Biophys. Res. Commun.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15358107/" } ], "x": 7740, "y": 2875.4607843137255, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159425", "label": "Cytosolic cholate and chenodeoxycholate are conjugated with Coenzyme A (SLC27A5 BACS)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-159425", "summary": "Cytosolic cholate and chenodeoxycholate are conjugated with Coenzyme A (SLC27A5 BACS) is a Reactome release 97 curated reaction event. The source record connects CCA,CDCA, ATP, CoA-SH to choloyl-CoA; chenodeoxycholoyl-CoA, AMP, PPi in cytosol · endoplasmic reticulum membrane.", "equation": "CCA,CDCA + ATP + CoA-SH → choloyl-CoA; chenodeoxycholoyl-CoA + AMP + PPi + H2O", "inputs": [ { "node_id": "R-ALL-194096", "reactome_id": "R-ALL-194096", "label": "CCA,CDCA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194073", "reactome_id": "R-ALL-194073", "label": "choloyl-CoA; chenodeoxycholoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of SLC27A5 [endoplasmic reticulum membrane]", "activity_id": "192026", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "Q9Y2P5" ] } ], "citations": [ { "id": "PMID:11980911", "title": "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980911/" } ], "x": 5008.666666666667, "y": 1683.2954545454545, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159431", "label": "Cytosolic chenodeoxycholoyl-CoA or choloyl-CoA are conjugated with glycine or taurine", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-159431", "summary": "Cytosolic chenodeoxycholoyl-CoA or choloyl-CoA are conjugated with glycine or taurine is a Reactome release 97 curated reaction event. The source record connects choloyl-CoA; chenodeoxycholoyl-CoA, Gly, TAU to bile salts, CoA-SH in cytosol.", "equation": "choloyl-CoA; chenodeoxycholoyl-CoA + Gly, TAU → bile salts + CoA-SH", "inputs": [ { "node_id": "R-ALL-194073", "reactome_id": "R-ALL-194073", "label": "choloyl-CoA; chenodeoxycholoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-194069", "reactome_id": "R-ALL-194069", "label": "Gly, TAU", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193416", "reactome_id": "R-ALL-193416", "label": "bile salts", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyltransferase activity of BAAT [cytosol]", "activity_id": "159410", "ec_numbers": [ "2.3" ], "accessions": [ "Q14032" ] } ], "citations": [ { "id": "PMID:8034703", "title": "Glycine and taurine conjugation of bile acids by a single enzyme. Molecular cloning and expression of human liver bile acid CoA:amino acid N-acyltransferase.", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8034703/" }, { "id": "PMID:2037576", "title": "Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from human liver", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2037576/" }, { "id": "PMID:10884298", "title": "Subcellular organization of bile acid amidation in human liver: a key issue in regulating the biosynthesis of bile salts", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10884298/" } ], "x": 4730, "y": 1742.2348484848485, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159443", "label": "benzoate + Coenzyme A + ATP =\u003e benzoyl-CoA + AMP + pyrophosphate", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:10133" ], "source_url": "https://reactome.org/content/detail/R-HSA-159443", "summary": "benzoate + Coenzyme A + ATP =\u003e benzoyl-CoA + AMP + pyrophosphate is a Reactome release 97 curated reaction event. The source record connects BENZA, ATP, CoA-SH to BEZ-CoA, AMP, PPi in mitochondrial matrix and cross-references RHEA:10133.", "equation": "BENZA + ATP + CoA-SH → BEZ-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-159446", "reactome_id": "R-ALL-159446", "label": "BENZA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30746", "formula": "C7H6O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-159447", "reactome_id": "R-ALL-159447", "label": "BEZ-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57369", "formula": "C28H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "CoA-ligase activity of xenobiotic/medium-chain fatty acid:CoA ligases [mitochondrial matrix]", "activity_id": "177146", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12616642", "title": "Isolation, sequencing, and expression of a cDNA for the HXM-A form of xenobiotic/medium-chain fatty acid:CoA ligase from human liver mitochondria", "journal": "J Biochem Mol Toxicol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12616642/" }, { "id": "PMID:10434065", "title": "Characterization of the CoA ligases of human liver mitochondria catalyzing the activation of short- and medium-chain fatty acids and xenobiotic carboxylic acids", "journal": "Biochim Biophys Acta", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10434065/" } ], "x": 7740, "y": 2837.323529411765, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159566", "label": "benzoyl-CoA + glycine =\u003e benzoyl glycine (hippuric acid) + Coenzyme A", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18494" ], "source_url": "https://reactome.org/content/detail/R-HSA-159566", "summary": "benzoyl-CoA + glycine =\u003e benzoyl glycine (hippuric acid) + Coenzyme A is a Reactome release 97 curated reaction event. The source record connects BEZ-CoA, Gly to HIPA, CoA-SH in mitochondrial matrix and cross-references RHEA:18494.", "equation": "BEZ-CoA + Gly → HIPA + CoA-SH", "inputs": [ { "node_id": "R-ALL-159447", "reactome_id": "R-ALL-159447", "label": "BEZ-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57369", "formula": "C28H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159564", "reactome_id": "R-ALL-159564", "label": "HIPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:606565", "formula": "C9H8NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycine N-benzoyltransferase activity of GLYAT-like proteins [mitochondrial matrix]", "activity_id": "159547", "ec_numbers": [ "2.3.1.71" ], "accessions": [] } ], "citations": [ { "id": "PMID:931988", "title": "Identification of separate acyl- CoA:glycine and acyl-CoA:L-glutamine N-acyltransferase activities in mitochondrial fractions from liver of rhesus monkey and man", "journal": "J Biol Chem", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/931988/" }, { "id": "PMID:7802672", "title": "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver", "journal": "Biochem Biophys Res Commun", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7802672/" } ], "x": 8018.666666666667, "y": 2875.4607843137255, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159567", "label": "ACSM2B-like proteins transform ST to ST-CoA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:61669" ], "source_url": "https://reactome.org/content/detail/R-HSA-159567", "summary": "ACSM2B-like proteins transform ST to ST-CoA is a Reactome release 97 curated reaction event. The source record connects ST, ATP, CoA-SH to ST-CoA, AMP, PPi in mitochondrial matrix and cross-references RHEA:61669.", "equation": "ST + ATP + CoA-SH → ST-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-159559", "reactome_id": "R-ALL-159559", "label": "ST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30762", "formula": "C7H5O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-159557", "reactome_id": "R-ALL-159557", "label": "ST-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32587", "formula": "C28H40N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "CoA-ligase activity of ACSM2B-like proteins [mitochondrial matrix]", "activity_id": "159441", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:5862532", "title": "Pharmacokinetics of salicylate elimination in man", "journal": "J Pharm Sci", "year": 1965, "url": "https://pubmed.ncbi.nlm.nih.gov/5862532/" }, { "id": "PMID:12616642", "title": "Isolation, sequencing, and expression of a cDNA for the HXM-A form of xenobiotic/medium-chain fatty acid:CoA ligase from human liver mitochondria", "journal": "J Biochem Mol Toxicol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12616642/" } ], "x": 8018.666666666667, "y": 2837.323529411765, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-159574", "label": "GLYAT-like proteins transfer glycine to ST-CoA to form SUA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:19870" ], "source_url": "https://reactome.org/content/detail/R-HSA-159574", "summary": "GLYAT-like proteins transfer glycine to ST-CoA to form SUA is a Reactome release 97 curated reaction event. The source record connects ST-CoA, Gly to SUA, CoA-SH in mitochondrial matrix and cross-references RHEA:19870.", "equation": "ST-CoA + Gly → SUA + CoA-SH", "inputs": [ { "node_id": "R-ALL-159557", "reactome_id": "R-ALL-159557", "label": "ST-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32587", "formula": "C28H40N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159572", "reactome_id": "R-ALL-159572", "label": "SUA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:9008", "formula": "C9H9NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycine N-benzoyltransferase activity of GLYAT-like proteins [mitochondrial matrix]", "activity_id": "159547", "ec_numbers": [ "2.3.1.71" ], "accessions": [] } ], "citations": [ { "id": "PMID:931988", "title": "Identification of separate acyl- CoA:glycine and acyl-CoA:L-glutamine N-acyltransferase activities in mitochondrial fractions from liver of rhesus monkey and man", "journal": "J Biol Chem", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/931988/" }, { "id": "PMID:7802672", "title": "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver", "journal": "Biochem Biophys Res Commun", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7802672/" } ], "x": 7461.333333333333, "y": 2883.0882352941176, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1602368", "label": "PG is hydrolyzed to 1-acyl LPG by PLA2[16]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1602368", "summary": "PG is hydrolyzed to 1-acyl LPG by PLA2[16] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PG, H2O to 1-acyl LPG, LCFA(-) in extracellular region · plasma membrane.", "equation": "PG + H2O → 1-acyl LPG + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1602402", "reactome_id": "R-ALL-1602402", "label": "PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1602415", "reactome_id": "R-ALL-1602415", "label": "1-acyl LPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62747", "formula": "C7H14O9PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(16) [extracellular region]", "activity_id": "1602353", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:11031251", "title": "Cloning and recombinant expression of a structurally novel human secreted phospholipase A2", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11031251/" }, { "id": "PMID:7060561", "title": "Studies on prophospholipase A2 and its enzyme from human pancreatic juice. Catalytic properties and sequence of the N-terminal region", "journal": "Eur J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7060561/" }, { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:2925608", "title": "Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2925608/" }, { "id": "PMID:10455175", "title": "Cloning and characterization of novel mouse and human secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10455175/" }, { "id": "PMID:10681567", "title": "Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681567/" } ], "x": 2938.666666666667, "y": 1780.7876712328766, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1602374", "label": "PS is hydrolyzed to 1-acyl LPS by PLA2[15]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1602374", "summary": "PS is hydrolyzed to 1-acyl LPS by PLA2[15] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PS, H2O to 1-acyl LPS, LCFA(-) in extracellular region · plasma membrane.", "equation": "PS + H2O → 1-acyl LPS + LCFA(-)", "inputs": [ { "node_id": "R-ALL-33625", "reactome_id": "R-ALL-33625", "label": "PS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1602419", "reactome_id": "R-ALL-1602419", "label": "1-acyl LPS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:52603", "formula": "C7H13NO9PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(15) [extracellular region]", "activity_id": "1602403", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:10455175", "title": "Cloning and characterization of novel mouse and human secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10455175/" }, { "id": "PMID:7060561", "title": "Studies on prophospholipase A2 and its enzyme from human pancreatic juice. Catalytic properties and sequence of the N-terminal region", "journal": "Eur J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7060561/" }, { "id": "PMID:2925608", "title": "Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2925608/" }, { "id": "PMID:10681567", "title": "Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681567/" }, { "id": "PMID:11112443", "title": "Cloning and recombinant expression of human group IIF-secreted phospholipase A(2)", "journal": "Biochem Biophys Res Commun", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11112443/" } ], "x": 2381.3333333333335, "y": 1786.1164383561645, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1602377", "label": "PI is hydrolyzed to 1-acyl LPI by PLA2[15]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1602377", "summary": "PI is hydrolyzed to 1-acyl LPI by PLA2[15] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PI, H2O to 1-acyl LPI, LCFA(-) in extracellular region · plasma membrane.", "equation": "PI + H2O → 1-acyl LPI + LCFA(-)", "inputs": [ { "node_id": "R-ALL-426658", "reactome_id": "R-ALL-426658", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1602420", "reactome_id": "R-ALL-1602420", "label": "1-acyl LPI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28914", "formula": "C10H18O12PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(15) [extracellular region]", "activity_id": "1602403", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:10455175", "title": "Cloning and characterization of novel mouse and human secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10455175/" }, { "id": "PMID:7060561", "title": "Studies on prophospholipase A2 and its enzyme from human pancreatic juice. Catalytic properties and sequence of the N-terminal region", "journal": "Eur J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7060561/" }, { "id": "PMID:2925608", "title": "Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2925608/" }, { "id": "PMID:10681567", "title": "Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681567/" }, { "id": "PMID:11112443", "title": "Cloning and recombinant expression of human group IIF-secreted phospholipase A(2)", "journal": "Biochem Biophys Res Commun", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11112443/" } ], "x": 2660, "y": 1786.1164383561645, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1602398", "label": "PE is hydrolyzed to 1-acyl LPE by PLA2[16]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1602398", "summary": "PE is hydrolyzed to 1-acyl LPE by PLA2[16] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PE, H2O to 1-acyl LPE, LCFA(-) in extracellular region · plasma membrane.", "equation": "PE + H2O → 1-acyl LPE + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1602375", "reactome_id": "R-ALL-1602375", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1602372", "reactome_id": "R-ALL-1602372", "label": "1-acyl LPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29017", "formula": "C6H13NO7PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(16) [extracellular region]", "activity_id": "1602353", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:11031251", "title": "Cloning and recombinant expression of a structurally novel human secreted phospholipase A2", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11031251/" }, { "id": "PMID:7060561", "title": "Studies on prophospholipase A2 and its enzyme from human pancreatic juice. Catalytic properties and sequence of the N-terminal region", "journal": "Eur J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7060561/" }, { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:2925608", "title": "Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2925608/" }, { "id": "PMID:10455175", "title": "Cloning and characterization of novel mouse and human secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10455175/" }, { "id": "PMID:10681567", "title": "Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681567/" } ], "x": 2938.666666666667, "y": 1786.1164383561645, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1602399", "label": "PC is hydrolyzed to 1-acyl LPC by PLB1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1602399", "summary": "PC is hydrolyzed to 1-acyl LPC by PLB1 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PC, H2O to 1-acyl LPC, LCFA(-) in extracellular region · plasma membrane.", "equation": "PC + H2O → 1-acyl LPC + LCFA(-)", "inputs": [ { "node_id": "R-ALL-429802", "reactome_id": "R-ALL-429802", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1602390", "reactome_id": "R-ALL-1602390", "label": "1-acyl LPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLB1 [plasma membrane]", "activity_id": "1602379", "ec_numbers": [ "3.1.1.4" ], "accessions": [ "Q6P1J6" ] } ], "citations": [ { "id": "PMID:12150957", "title": "Human epidermis is a novel site of phospholipase B expression", "journal": "Biochem Biophys Res Commun", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12150957/" }, { "id": "PMID:1618844", "title": "Substrate specificity of phospholipase B from guinea pig intestine. A glycerol ester lipase with broad specificity", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1618844/" } ], "x": 2381.3333333333335, "y": 1791.445205479452, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1602417", "label": "PC is hydrolyzed to 1-acyl LPC by PLA2[16]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1602417", "summary": "PC is hydrolyzed to 1-acyl LPC by PLA2[16] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PC, H2O to 1-acyl LPC, LCFA(-) in extracellular region · plasma membrane.", "equation": "PC + H2O → 1-acyl LPC + LCFA(-)", "inputs": [ { "node_id": "R-ALL-429802", "reactome_id": "R-ALL-429802", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1602390", "reactome_id": "R-ALL-1602390", "label": "1-acyl LPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17504", "formula": "C9H20NO7PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(16) [extracellular region]", "activity_id": "1602353", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:11031251", "title": "Cloning and recombinant expression of a structurally novel human secreted phospholipase A2", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11031251/" }, { "id": "PMID:7060561", "title": "Studies on prophospholipase A2 and its enzyme from human pancreatic juice. Catalytic properties and sequence of the N-terminal region", "journal": "Eur J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7060561/" }, { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:2925608", "title": "Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2925608/" }, { "id": "PMID:10455175", "title": "Cloning and characterization of novel mouse and human secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10455175/" }, { "id": "PMID:10681567", "title": "Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681567/" } ], "x": 2660, "y": 1791.445205479452, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1602446", "label": "PA is hydrolyzed to 1-acyl LPA by PLA2[15]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1602446", "summary": "PA is hydrolyzed to 1-acyl LPA by PLA2[15] is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PA, H2O to 1-acyl LPA, LCFA(-) in extracellular region · plasma membrane.", "equation": "PA + H2O → 1-acyl LPA + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1602448", "reactome_id": "R-ALL-1602448", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1602447", "reactome_id": "R-ALL-1602447", "label": "1-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2(15) [extracellular region]", "activity_id": "1602403", "ec_numbers": [ "3.1.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12359733", "title": "Interfacial kinetic and binding properties of the complete set of human and mouse groups I, II, V, X, and XII secreted phospholipases A2", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359733/" }, { "id": "PMID:10455175", "title": "Cloning and characterization of novel mouse and human secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10455175/" }, { "id": "PMID:7060561", "title": "Studies on prophospholipase A2 and its enzyme from human pancreatic juice. Catalytic properties and sequence of the N-terminal region", "journal": "Eur J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7060561/" }, { "id": "PMID:2925608", "title": "Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2925608/" }, { "id": "PMID:10681567", "title": "Structures, enzymatic properties, and expression of novel human and mouse secretory phospholipase A(2)s", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681567/" }, { "id": "PMID:11112443", "title": "Cloning and recombinant expression of human group IIF-secreted phospholipase A(2)", "journal": "Biochem Biophys Res Commun", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11112443/" } ], "x": 2938.666666666667, "y": 1791.445205479452, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1605591", "label": "GBA1:SAPC hydrolyzes GlcCer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal membrane · lysosomal lumen", "compartment_ids": [ "GO:0005765", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605591", "summary": "GBA1:SAPC hydrolyzes GlcCer is a Reactome release 97 curated reaction event. The source record connects GlcCer, H2O to CERA, Glc in lysosomal membrane · lysosomal lumen.", "equation": "GlcCer + H2O → CERA + Glc", "inputs": [ { "node_id": "R-ALL-1605592", "reactome_id": "R-ALL-1605592", "label": "GlcCer", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:22801", "formula": "C25H46NO8R", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1605673", "reactome_id": "R-ALL-1605673", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605745", "reactome_id": "R-ALL-1605745", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucosylceramidase activity of GBA1:SAPC [lysosomal membrane]", "activity_id": "1605702", "ec_numbers": [ "3.2.1.45" ], "accessions": [] } ], "citations": [ { "id": "PMID:3456607", "title": "Human acid beta-glucosidase: isolation and amino acid sequence of a peptide containing the catalytic site", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3456607/" }, { "id": "PMID:10781797", "title": "Further studies on the reconstitution of glucosylceramidase activity by Sap C and anionic phospholipids", "journal": "FEBS Lett", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10781797/" }, { "id": "PMID:8889578", "title": "Glucocerebrosidase (Gaucher disease)", "journal": "Hum Mutat", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8889578/" }, { "id": "PMID:28126847", "title": "Lipids regulate the hydrolysis of membrane bound glucosylceramide by lysosomal β-glucocerebrosidase", "journal": "J Lipid Res", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28126847/" } ], "x": 3321.3333333333335, "y": 1734.9807692307693, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1605595", "label": "bHEXA hydrolyzes GM2A:GM2 to GM2A:GM3", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605595", "summary": "bHEXA hydrolyzes GM2A:GM2 to GM2A:GM3 is a Reactome release 97 curated reaction event. The source record connects GM2A:GM2, H2O to GalNAc, GM3, GM2A(32-193) in lysosomal lumen.", "equation": "GM2A:GM2 + H2O → GalNAc + GM3 + GM2A(32-193)", "inputs": [ { "node_id": "R-HSA-1605600", "reactome_id": "R-HSA-1605600", "label": "GM2A:GM2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1605658", "reactome_id": "R-ALL-1605658", "label": "GalNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28037", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605641", "reactome_id": "R-ALL-1605641", "label": "GM3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60065", "formula": "C42H72N2O21R", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-1605614", "reactome_id": "R-HSA-1605614", "label": "GM2A(32-193)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXA [lysosomal lumen]", "activity_id": "1605773", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:2971395", "title": "Oligosaccharide structure and amino acid sequence of the major glycopeptides of mature human beta-hexosaminidase", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2971395/" }, { "id": "PMID:1720305", "title": "Molecular basis of an adult form of beta-hexosaminidase B deficiency with motor neuron disease", "journal": "Biochem Biophys Res Commun", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1720305/" }, { "id": "PMID:2970528", "title": "A point mutation in the coding sequence of the beta-hexosaminidase alpha gene results in defective processing of the enzyme protein in an unusual GM2-gangliosidosis variant", "journal": "J Neurochem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2970528/" }, { "id": "PMID:527942", "title": "Purification and characterization of an activator protein for the degradation of glycolipids GM2 and GA2 by hexosaminidase A", "journal": "Hoppe Seylers Z Physiol Chem", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/527942/" }, { "id": "PMID:16698036", "title": "Crystallographic structure of human beta-hexosaminidase A: interpretation of Tay-Sachs mutations and loss of GM2 ganglioside hydrolysis", "journal": "J Mol Biol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16698036/" }, { "id": "PMID:9217013", "title": "Formation of a ternary complex between GM2 activator protein, GM2 ganglioside and hexosaminidase A", "journal": "Biochim Biophys Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9217013/" } ], "x": 3321.3333333333335, "y": 1779.8653846153845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1605624", "label": "Beta-galactosidases hydrolyse mobilized GM1 to mobilized GM2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen · lysosomal membrane", "compartment_ids": [ "GO:0043202", "GO:0005765" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605624", "summary": "Beta-galactosidases hydrolyse mobilized GM1 to mobilized GM2 is a Reactome release 97 curated reaction event. The source record connects H2O, mobilized GM1 to Gal, mobilized GM2 in lysosomal lumen · lysosomal membrane.", "equation": "H2O + mobilized GM1 → Gal + mobilized GM2", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9840644", "reactome_id": "R-HSA-9840644", "label": "mobilized GM1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605637", "reactome_id": "R-ALL-1605637", "label": "Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840646", "reactome_id": "R-HSA-9840646", "label": "mobilized GM2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "beta-galactosidase activity of BGAL [lysosomal lumen]", "activity_id": "1605789", "ec_numbers": [ "3.2.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:5822067", "title": "Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase", "journal": "Biochem J", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5822067/" }, { "id": "PMID:1907800", "title": "Human beta-galactosidase gene mutations in GM1-gangliosidosis: a common mutation among Japanese adult/chronic cases", "journal": "Am J Hum Genet", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1907800/" }, { "id": "PMID:10942779", "title": "Degradation of membrane-bound ganglioside GM1. Stimulation by bis(monoacylglycero)phosphate and the activator proteins SAP-B and GM2-AP", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10942779/" }, { "id": "PMID:18524657", "title": "GM1 gangliosidosis: review of clinical, molecular, and therapeutic aspects", "journal": "Mol Genet Metab", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18524657/" } ], "x": 3600, "y": 1824.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1605632", "label": "bHEXA,bHEXB hydrolyze PSAP(195-273):Gb4Cer:PE", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605632", "summary": "bHEXA,bHEXB hydrolyze PSAP(195-273):Gb4Cer:PE is a Reactome release 97 curated reaction event. The source record connects H2O, PSAP(195-273):Gb4Cer:PE to GalNAc, PSAP(195-273):Gb3Cer:PE in lysosomal lumen.", "equation": "H2O + PSAP(195-273):Gb4Cer:PE → GalNAc + PSAP(195-273):Gb3Cer:PE", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9840842", "reactome_id": "R-HSA-9840842", "label": "PSAP(195-273):Gb4Cer:PE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605658", "reactome_id": "R-ALL-1605658", "label": "GalNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28037", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840848", "reactome_id": "R-HSA-9840848", "label": "PSAP(195-273):Gb3Cer:PE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXA,bHEXB [lysosomal lumen]", "activity_id": "1605761", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:2971395", "title": "Oligosaccharide structure and amino acid sequence of the major glycopeptides of mature human beta-hexosaminidase", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2971395/" }, { "id": "PMID:2965147", "title": "Proteolytic processing of pro-alpha and pro-beta precursors from human beta-hexosaminidase. Generation of the mature alpha and beta a beta b subunits", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2965147/" }, { "id": "PMID:12662933", "title": "Crystal structure of human beta-hexosaminidase B: understanding the molecular basis of Sandhoff and Tay-Sachs disease", "journal": "J Mol Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12662933/" }, { "id": "PMID:5135907", "title": "Enzyme alterations and lipid storage in three variants of Tay-Sachs disease", "journal": "J Neurochem", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/5135907/" }, { "id": "PMID:29747811", "title": "Ganglioside Metabolism in Health and Disease", "journal": "Prog Mol Biol Transl Sci", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29747811/" }, { "id": "PMID:29802621", "title": "Emerging concepts of ganglioside metabolism", "journal": "FEBS Lett", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29802621/" } ], "x": 3878.666666666667, "y": 1824.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1605717", "label": "GM2A binds and mobilizes ligands", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen · multivesicular body, internal vesicle membrane", "compartment_ids": [ "GO:0043202", "GO:0097488" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605717", "summary": "GM2A binds and mobilizes ligands is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects GM2A(32-193), GM2A ligands to GM2A:Ligands in lysosomal lumen · multivesicular body, internal vesicle membrane.", "equation": "GM2A(32-193) + GM2A ligands → GM2A:Ligands", "inputs": [ { "node_id": "R-HSA-1605614", "reactome_id": "R-HSA-1605614", "label": "GM2A(32-193)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9840941", "reactome_id": "R-ALL-9840941", "label": "GM2A ligands", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "multivesicular body, internal vesicle membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9840631", "reactome_id": "R-HSA-9840631", "label": "GM2A:Ligands", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "sphingolipid activator protein activity of GM2A(32-193) [lysosomal lumen]", "activity_id": "1861790", "ec_numbers": null, "accessions": [ "P17900" ] } ], "citations": [ { "id": "PMID:12909021", "title": "Structural analysis of lipid complexes of GM2-activator protein", "journal": "J Mol Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12909021/" }, { "id": "PMID:1915858", "title": "A mutation in the gene of a glycolipid-binding protein (GM2 activator) that causes GM2-gangliosidosis variant AB", "journal": "FEBS Lett", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1915858/" }, { "id": "PMID:10942779", "title": "Degradation of membrane-bound ganglioside GM1. Stimulation by bis(monoacylglycero)phosphate and the activator proteins SAP-B and GM2-AP", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10942779/" }, { "id": "PMID:29802621", "title": "Emerging concepts of ganglioside metabolism", "journal": "FEBS Lett", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29802621/" } ], "x": 3600, "y": 1869.6346153846155, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1605723", "label": "NEU2 cleaves GM3 to form LacCer (cytosol)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605723", "summary": "NEU2 cleaves GM3 to form LacCer (cytosol) is a Reactome release 97 curated reaction event. The source record connects GM3, H2O to Neu5Ac, LacCer in cytosol.", "equation": "GM3 + H2O → Neu5Ac + LacCer", "inputs": [ { "node_id": "R-ALL-1605590", "reactome_id": "R-ALL-1605590", "label": "GM3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60065", "formula": "C42H72N2O21R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-428633", "reactome_id": "R-ALL-428633", "label": "Neu5Ac", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35418", "formula": "C11H18NO9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605647", "reactome_id": "R-ALL-1605647", "label": "LacCer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195546", "formula": "C16H27NO13R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "exo-alpha-sialidase activity of NEU2 [cytosol]", "activity_id": "1605672", "ec_numbers": [ "3.2.1.18" ], "accessions": [ "Q9Y3R4" ] } ], "citations": [ { "id": "PMID:10191093", "title": "Cloning and characterization of NEU2, a human gene homologous to rodent soluble sialidases", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10191093/" } ], "x": 3600, "y": 1734.9807692307693, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1605724", "label": "NEU1,4 hydrolyze PSAP(195-273):GM3:PE", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605724", "summary": "NEU1,4 hydrolyze PSAP(195-273):GM3:PE is a Reactome release 97 curated reaction event. The source record connects H2O, PSAP(195-273):GM3:PE to Neu5Ac, PSAP(195-273):LacCer:PE in lysosomal lumen.", "equation": "H2O + PSAP(195-273):GM3:PE → Neu5Ac + PSAP(195-273):LacCer:PE", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9840821", "reactome_id": "R-HSA-9840821", "label": "PSAP(195-273):GM3:PE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-428639", "reactome_id": "R-ALL-428639", "label": "Neu5Ac", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35418", "formula": "C11H18NO9", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840818", "reactome_id": "R-HSA-9840818", "label": "PSAP(195-273):LacCer:PE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "exo-alpha-sialidase activity of NEU1,4 [lysosomal lumen]", "activity_id": "1605780", "ec_numbers": [ "3.2.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:8985184", "title": "Characterization of human lysosomal neuraminidase defines the molecular basis of the metabolic storage disorder sialidosis", "journal": "Genes Dev", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8985184/" }, { "id": "PMID:3136930", "title": "Expression of cDNA encoding the human \"protective protein\" associated with lysosomal beta-galactosidase and neuraminidase: homology to yeast proteases", "journal": "Cell", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3136930/" }, { "id": "PMID:1756715", "title": "A mutation in a mild form of galactosialidosis impairs dimerization of the protective protein and renders it unstable", "journal": "EMBO J", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1756715/" }, { "id": "PMID:8591035", "title": "Three-dimensional structure of the human 'protective protein': structure of the precursor form suggests a complex activation mechanism", "journal": "Structure", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8591035/" }, { "id": "PMID:15213228", "title": "Neu4, a novel human lysosomal lumen sialidase, confers normal phenotype to sialidosis and galactosialidosis cells", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15213228/" }, { "id": "PMID:8370580", "title": "Prosaposin deficiency: further characterization of the sphingolipid activator protein-deficient sibs. Multiple glycolipid elevations (including lactosylceramidosis), partial enzyme deficiencies and ultrastructure of the skin in this generalized sphingolipid storage disease", "journal": "Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8370580/" } ], "x": 3321.3333333333335, "y": 1839.7115384615386, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1605736", "label": "GLA hydrolyzes PSAP(195-273):Gb3Cer:PE", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605736", "summary": "GLA hydrolyzes PSAP(195-273):Gb3Cer:PE is a Reactome release 97 curated reaction event. The source record connects H2O, PSAP(195-273):Gb3Cer:PE to Gal, PSAP(195-273):LacCer:PE in lysosomal lumen.", "equation": "H2O + PSAP(195-273):Gb3Cer:PE → Gal + PSAP(195-273):LacCer:PE", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9840848", "reactome_id": "R-HSA-9840848", "label": "PSAP(195-273):Gb3Cer:PE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605637", "reactome_id": "R-ALL-1605637", "label": "Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840818", "reactome_id": "R-HSA-9840818", "label": "PSAP(195-273):LacCer:PE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "alpha-galactosidase activity of GLA dimer [lysosomal lumen]", "activity_id": "1605696", "ec_numbers": [ "3.2.1.22" ], "accessions": [] } ], "citations": [ { "id": "PMID:3014515", "title": "Human alpha-galactosidase A: nucleotide sequence of a cDNA clone encoding the mature enzyme", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3014515/" }, { "id": "PMID:15003450", "title": "The molecular defect leading to Fabry disease: structure of human alpha-galactosidase", "journal": "J Mol Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15003450/" }, { "id": "PMID:7504405", "title": "Nature and frequency of mutations in the alpha-galactosidase A gene that cause Fabry disease", "journal": "Am J Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7504405/" }, { "id": "PMID:16595074", "title": "Fabry disease: identification of 50 novel alpha-galactosidase A mutations causing the classic phenotype and three-dimensional structural analysis of 29 missense mutations", "journal": "Hum Genomics", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16595074/" }, { "id": "PMID:8370580", "title": "Prosaposin deficiency: further characterization of the sphingolipid activator protein-deficient sibs. Multiple glycolipid elevations (including lactosylceramidosis), partial enzyme deficiencies and ultrastructure of the skin in this generalized sphingolipid storage disease", "journal": "Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8370580/" } ], "x": 3600, "y": 1839.7115384615386, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1605768", "label": "NEU3 cleaves GM3 to form LacCer (plasma membrane)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605768", "summary": "NEU3 cleaves GM3 to form LacCer (plasma membrane) is a Reactome release 97 curated reaction event. The source record connects GM3, H2O to Neu5Ac, LacCer in cytosol · plasma membrane.", "equation": "GM3 + H2O → Neu5Ac + LacCer", "inputs": [ { "node_id": "R-ALL-1605649", "reactome_id": "R-ALL-1605649", "label": "GM3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60065", "formula": "C42H72N2O21R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-428633", "reactome_id": "R-ALL-428633", "label": "Neu5Ac", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35418", "formula": "C11H18NO9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605618", "reactome_id": "R-ALL-1605618", "label": "LacCer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195546", "formula": "C16H27NO13R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "exo-alpha-sialidase activity of NEU3 [plasma membrane]", "activity_id": "1605760", "ec_numbers": [ "3.2.1.18" ], "accessions": [ "Q9UQ49" ] } ], "citations": [ { "id": "PMID:10861246", "title": "Identification and expression of NEU3, a novel human sialidase associated to the plasma membrane", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10861246/" } ], "x": 3878.666666666667, "y": 1734.9807692307693, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1605797", "label": "SMPD1 hydrolyzes SPHM", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1605797", "summary": "SMPD1 hydrolyzes SPHM is a Reactome release 97 curated reaction event. The source record connects SPHM d18:1, H2O to CERA, ChoP, H+ in lysosomal lumen.", "equation": "SPHM d18:1 + H2O → CERA + ChoP + H+", "inputs": [ { "node_id": "R-ALL-1605713", "reactome_id": "R-ALL-1605713", "label": "SPHM d18:1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17636", "formula": "C24H48N2O6PR", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1605673", "reactome_id": "R-ALL-1605673", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605750", "reactome_id": "R-ALL-1605750", "label": "ChoP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:295975", "formula": "C5H13NO4P", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428040", "reactome_id": "R-ALL-428040", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sphingomyelin phosphodiesterase activity of SMPD1 [lysosomal lumen]", "activity_id": "1605754", "ec_numbers": [ "3.1.4.12" ], "accessions": [ "P17405" ] } ], "citations": [ { "id": "PMID:1840600", "title": "Human acid sphingomyelinase. Isolation, nucleotide sequence and expression of the full-length and alternatively spliced cDNAs", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1840600/" }, { "id": "PMID:1740330", "title": "Structural organization and complete nucleotide sequence of the gene encoding human acid sphingomyelinase (SMPD1)", "journal": "Genomics", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1740330/" }, { "id": "PMID:1718266", "title": "Molecular basis of acid sphingomyelinase deficiency in a patient with Niemann-Pick disease type A", "journal": "Biochem Biophys Res Commun", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1718266/" }, { "id": "PMID:19405096", "title": "Identification and characterization of SMPD1 mutations causing Niemann-Pick types A and B in Spanish patients", "journal": "Hum Mutat", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19405096/" } ], "x": 3600, "y": 1675.1346153846155, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1606273", "label": "SMPD2 and 3 hydrolyze sphingomyelin (plasma membrane)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1606273", "summary": "SMPD2 and 3 hydrolyze sphingomyelin (plasma membrane) is a Reactome release 97 curated reaction event. The source record connects SPHM d18:1, H2O to CERA, ChoP, H+ in plasma membrane · cytosol.", "equation": "SPHM d18:1 + H2O → CERA + ChoP + H+", "inputs": [ { "node_id": "R-ALL-429776", "reactome_id": "R-ALL-429776", "label": "SPHM d18:1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17636", "formula": "C24H48N2O6PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-429807", "reactome_id": "R-ALL-429807", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1610735", "reactome_id": "R-ALL-1610735", "label": "ChoP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:295975", "formula": "C5H13NO4P", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sphingomyelin phosphodiesterase activity of SMPD2,3:Mg2+ [plasma membrane]", "activity_id": "1606275", "ec_numbers": [ "3.1.4.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:9520418", "title": "Cloned mammalian neutral sphingomyelinase: functions in sphingolipid signaling?", "journal": "Proc Natl Acad Sci U S A", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9520418/" }, { "id": "PMID:10823942", "title": "Cloning and characterization of the mammalian brain-specific, Mg2+-dependent neutral sphingomyelinase", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10823942/" }, { "id": "PMID:12566438", "title": "Biochemical properties of mammalian neutral sphingomyelinase 2 and its role in sphingolipid metabolism", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12566438/" } ], "x": 3878.666666666667, "y": 1675.1346153846155, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1606288", "label": "SMPD4 hydrolyzes sphingomyelin (ER membrane)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1606288", "summary": "SMPD4 hydrolyzes sphingomyelin (ER membrane) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects SPHM d18:1, H2O to CERA, ChoP, H+ in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "SPHM d18:1 + H2O → CERA + ChoP + H+", "inputs": [ { "node_id": "R-ALL-1606283", "reactome_id": "R-ALL-1606283", "label": "SPHM d18:1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17636", "formula": "C24H48N2O6PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-428256", "reactome_id": "R-ALL-428256", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1606280", "reactome_id": "R-ALL-1606280", "label": "ChoP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18132", "formula": "C5H15NO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sphingomyelin phosphodiesterase activity of SMPD4:Mg2+ [endoplasmic reticulum membrane]", "activity_id": "1606279", "ec_numbers": [ "3.1.4.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:16517606", "title": "Novel tumor necrosis factor-responsive mammalian neutral sphingomyelinase-3 is a C-tail-anchored protein", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16517606/" }, { "id": "PMID:31495489", "title": "Loss of SMPD4 Causes a Developmental Disorder Characterized by Microcephaly and Congenital Arthrogryposis", "journal": "Am J Hum Genet", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31495489/" } ], "x": 3321.3333333333335, "y": 1690.0961538461538, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1606312", "label": "GLB1 hydrolyzes SapB/C:LacCer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1606312", "summary": "GLB1 hydrolyzes SapB/C:LacCer is a Reactome release 97 curated reaction event. The source record connects H2O, Sap B/C:LacCer to Gal, Sap B/C:GlcCer in lysosomal lumen.", "equation": "H2O + Sap B/C:LacCer → Gal + Sap B/C:GlcCer", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9840969", "reactome_id": "R-HSA-9840969", "label": "Sap B/C:LacCer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605637", "reactome_id": "R-ALL-1605637", "label": "Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840964", "reactome_id": "R-HSA-9840964", "label": "Sap B/C:GlcCer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "beta-galactosidase activity of BGAL [lysosomal lumen]", "activity_id": "1605789", "ec_numbers": [ "3.2.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:5822067", "title": "Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase", "journal": "Biochem J", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5822067/" }, { "id": "PMID:29802621", "title": "Emerging concepts of ganglioside metabolism", "journal": "FEBS Lett", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29802621/" }, { "id": "PMID:8370580", "title": "Prosaposin deficiency: further characterization of the sphingolipid activator protein-deficient sibs. Multiple glycolipid elevations (including lactosylceramidosis), partial enzyme deficiencies and ultrastructure of the skin in this generalized sphingolipid storage disease", "journal": "Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8370580/" } ], "x": 3878.666666666667, "y": 1839.7115384615386, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1606564", "label": "GALC hydrolyzes GalCer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1606564", "summary": "GALC hydrolyzes GalCer is a Reactome release 97 curated reaction event. The source record connects H2O, GalCer to CERA, Glc in lysosomal lumen.", "equation": "H2O + GalCer → CERA + Glc", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1606573", "reactome_id": "R-ALL-1606573", "label": "GalCer", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36498", "formula": "C10H17NO8R2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605673", "reactome_id": "R-ALL-1605673", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605745", "reactome_id": "R-ALL-1605745", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "galactosylceramidase activity of GALC [lysosomal lumen]", "activity_id": "1606568", "ec_numbers": [ "3.2.1.46" ], "accessions": [ "P54803" ] } ], "citations": [ { "id": "PMID:7852280", "title": "Purification and characterization of galactocerebrosidase from human lymphocytes", "journal": "J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7852280/" }, { "id": "PMID:9338580", "title": "Molecular genetics of Krabbe disease (globoid cell leukodystrophy): diagnostic and clinical implications", "journal": "Hum Mutat", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9338580/" }, { "id": "PMID:8281145", "title": "Cloning and expression of cDNA encoding human galactocerebrosidase, the enzyme deficient in globoid cell leukodystrophy", "journal": "Hum Mol Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8281145/" }, { "id": "PMID:8399327", "title": "Galactocerebrosidase from human urine: purification and partial characterization", "journal": "Biochim Biophys Acta", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8399327/" }, { "id": "PMID:2717620", "title": "Saposin A: second cerebrosidase activator protein", "journal": "Proc Natl Acad Sci U S A", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2717620/" }, { "id": "PMID:15773042", "title": "A mutation in the saposin A coding region of the prosaposin gene in an infant presenting as Krabbe disease: first report of saposin A deficiency in humans", "journal": "Mol Genet Metab", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15773042/" } ], "x": 3321.3333333333335, "y": 1749.9423076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1606583", "label": "ASAH2 hydrolyzes ceramide (plasma membrane)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:78455" ], "source_url": "https://reactome.org/content/detail/R-HSA-1606583", "summary": "ASAH2 hydrolyzes ceramide (plasma membrane) is a Reactome release 97 curated reaction event. The source record connects CERA, H2O to SPG, RCOO- in plasma membrane · cytosol and cross-references RHEA:78455.", "equation": "CERA + H2O → SPG + RCOO-", "inputs": [ { "node_id": "R-ALL-429807", "reactome_id": "R-ALL-429807", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1606609", "reactome_id": "R-ALL-1606609", "label": "SPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84410", "formula": "C3H9NO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1861754", "reactome_id": "R-ALL-1861754", "label": "RCOO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28868", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acylsphingosine amidohydrolase activity of ASAH2-like proteins [plasma membrane]", "activity_id": "1606595", "ec_numbers": [ "3.5.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:15845354", "title": "Subcellular localization of human neutral ceramidase expressed in HEK293 cells", "journal": "Biochem Biophys Res Commun", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15845354/" }, { "id": "PMID:16229686", "title": "Identification of a novel amidase motif in neutral ceramidase", "journal": "Biochem J", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16229686/" } ], "x": 3600, "y": 1600.326923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1606602", "label": "ASAH1 hydrolyzes ceramide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [ "RHEA:78456" ], "source_url": "https://reactome.org/content/detail/R-HSA-1606602", "summary": "ASAH1 hydrolyzes ceramide is a Reactome release 97 curated reaction event. The source record connects CERA, H2O to SPG, RCOO- in lysosomal lumen and cross-references RHEA:78456.", "equation": "CERA + H2O → SPG + RCOO-", "inputs": [ { "node_id": "R-ALL-1605673", "reactome_id": "R-ALL-1605673", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1606588", "reactome_id": "R-ALL-1606588", "label": "SPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84410", "formula": "C3H9NO2R", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1861779", "reactome_id": "R-ALL-1861779", "label": "RCOO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28868", "formula": "CO2R", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acylsphingosine amidohydrolase activity of ASAH1 [lysosomal lumen]", "activity_id": "1606585", "ec_numbers": [ "3.5.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:7744740", "title": "Purification, characterization, and biosynthesis of human acid ceramidase", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7744740/" }, { "id": "PMID:10993717", "title": "Human acid ceramidase gene: novel mutations in Farber disease", "journal": "Mol Genet Metab", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10993717/" }, { "id": "PMID:8955159", "title": "Molecular cloning and characterization of a full-length complementary DNA encoding human acid ceramidase. Identification Of the first molecular lesion causing Farber disease", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8955159/" }, { "id": "PMID:10406958", "title": "Structural and membrane-binding properties of saposin D", "journal": "Eur J Biochem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10406958/" }, { "id": "PMID:29692406", "title": "Structural basis for the activation of acid ceramidase", "journal": "Nat Commun", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29692406/" } ], "x": 3878.666666666667, "y": 1600.326923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1606789", "label": "ARSB hydrolyses DS", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1606789", "summary": "ARSB hydrolyses DS is a Reactome release 97 curated reaction event. The source record connects H2O, ChEBI:63519 chain to SO4(2-), ChEBI:63517 chain in lysosomal lumen.", "equation": "H2O + ChEBI:63519 chain → SO4(2-) + ChEBI:63517 chain", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2065231", "reactome_id": "R-ALL-2065231", "label": "ChEBI:63519 chain", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747399", "formula": "C45H68N2O46S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2065213", "reactome_id": "R-ALL-2065213", "label": "ChEBI:63517 chain", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747397", "formula": "C45H68N2O43S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acetylgalactosamine-4-sulfatase activity of ARSB:Ca2+ [lysosomal lumen]", "activity_id": "1606816", "ec_numbers": [ "3.1.6.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:738706", "title": "Arylsulphatase B, an exo-sulphatase for chondroitin 4-sulphate tetrasaccharide", "journal": "Hoppe Seylers Z Physiol Chem", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/738706/" }, { "id": "PMID:12888274", "title": "Crystal structure of a covalent intermediate of endogenous human arylsulfatase A", "journal": "J Inorg Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12888274/" }, { "id": "PMID:9521684", "title": "Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis", "journal": "Biochemistry", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9521684/" }, { "id": "PMID:7628016", "title": "A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency", "journal": "Cell", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7628016/" } ], "x": 2938.666666666667, "y": 912.9242424242424, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1606807", "label": "ARSA removes sulfate from PSAP(195-273):Sulfatide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1606807", "summary": "ARSA removes sulfate from PSAP(195-273):Sulfatide is a Reactome release 97 curated reaction event. The source record connects H2O, PSAP(195-273):Sulfatide:PE to GalCer, SO4(2-), PSAP(195-273) dimer in lysosomal lumen.", "equation": "H2O + PSAP(195-273):Sulfatide:PE → GalCer + SO4(2-) + PSAP(195-273) dimer + PE 30,32,34 + H+", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9840996", "reactome_id": "R-HSA-9840996", "label": "PSAP(195-273):Sulfatide:PE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606573", "reactome_id": "R-ALL-1606573", "label": "GalCer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36498", "formula": "C10H17NO8R2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840482", "reactome_id": "R-HSA-9840482", "label": "PSAP(195-273) dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9840939", "reactome_id": "R-ALL-9840939", "label": "PE 30,32,34", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-428040", "reactome_id": "R-ALL-428040", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cerebroside-sulfatase activity of active ARSA:Ca2+ [lysosomal lumen]", "activity_id": "1606828", "ec_numbers": [ "3.1.6.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:2562955", "title": "Cloning and expression of human arylsulfatase A", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2562955/" }, { "id": "PMID:1352993", "title": "Proteolytic processing of human lysosomal arylsulfatase A", "journal": "Biochim Biophys Acta", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1352993/" }, { "id": "PMID:12888274", "title": "Crystal structure of a covalent intermediate of endogenous human arylsulfatase A", "journal": "J Inorg Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12888274/" }, { "id": "PMID:9521684", "title": "Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis", "journal": "Biochemistry", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9521684/" }, { "id": "PMID:1678251", "title": "Mutations in the arylsulfatase A pseudodeficiency allele causing metachromatic leukodystrophy", "journal": "Am J Hum Genet", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1678251/" }, { "id": "PMID:1670590", "title": "Molecular basis of different forms of metachromatic leukodystrophy", "journal": "N Engl J Med", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1670590/" } ], "x": 3600, "y": 1749.9423076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1606839", "label": "ARSC hydrolyzes steroid sulfates", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1606839", "summary": "ARSC hydrolyzes steroid sulfates is a Reactome release 97 curated reaction event. The source record connects H2O, steroid sulfate to SO4(2-), steroid in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "H2O + steroid sulfate → SO4(2-) + steroid", "inputs": [ { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9841212", "reactome_id": "R-ALL-9841212", "label": "steroid sulfate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59696", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1609649", "reactome_id": "R-ALL-1609649", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9841230", "reactome_id": "R-ALL-9841230", "label": "steroid", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35341", "formula": "C19H31R", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steryl-sulfatase activity of active STS dimer [endoplasmic reticulum membrane]", "activity_id": "1606835", "ec_numbers": [ "3.1.6.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:6417417", "title": "Human placental steroid sulfatase: purification and properties", "journal": "J Steroid Biochem", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6417417/" }, { "id": "PMID:1606923", "title": "Purification and properties of steroid sulfatase from human placenta", "journal": "Endocrinol Jpn", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1606923/" }, { "id": "PMID:2953589", "title": "Purification and properties of arylsulfatase C from human placenta", "journal": "Enzyme", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2953589/" }, { "id": "PMID:2668275", "title": "Cloning and expression of human steroid-sulfatase. Membrane topology, glycosylation, and subcellular distribution in BHK-21 cells", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2668275/" }, { "id": "PMID:1539590", "title": "Identification of point mutations in the steroid sulfatase gene of three patients with X-linked ichthyosis", "journal": "Am J Hum Genet", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1539590/" }, { "id": "PMID:9252398", "title": "Characterization of point mutations in patients with X-linked ichthyosis. Effects on the structure and function of the steroid sulfatase protein", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9252398/" } ], "x": 4730, "y": 1712.7651515151515, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614336", "label": "SUMF2 binds SUMF1, inhibiting SUMF1-mediated activation of arylsulfatases", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1614336", "summary": "SUMF2 binds SUMF1, inhibiting SUMF1-mediated activation of arylsulfatases is a Reactome release 97 curated reaction event. The source record connects SUMF1, SUMF2 to SUMF1:SUMF2 in endoplasmic reticulum lumen.", "equation": "SUMF1 + SUMF2 → SUMF1:SUMF2", "inputs": [ { "node_id": "R-HSA-1614316", "reactome_id": "R-HSA-1614316", "label": "SUMF1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-1614305", "reactome_id": "R-HSA-1614305", "label": "SUMF2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1614330", "reactome_id": "R-HSA-1614330", "label": "SUMF1:SUMF2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "enzyme inhibitor activity of SUMF2 [endoplasmic reticulum lumen]", "activity_id": "9956914", "ec_numbers": null, "accessions": [ "Q8NBJ7" ] } ], "citations": [ { "id": "PMID:15708861", "title": "Expression, localization, structural, and functional characterization of pFGE, the paralog of the Calpha-formylglycine-generating enzyme", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15708861/" }, { "id": "PMID:15962010", "title": "Sulphatase activities are regulated by the interaction of sulphatase-modifying factor 1 with SUMF2", "journal": "EMBO Rep", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15962010/" }, { "id": "PMID:18508857", "title": "Multistep, sequential control of the trafficking and function of the multiple sulfatase deficiency gene product, SUMF1 by PDI, ERGIC-53 and ERp44", "journal": "Hum Mol Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18508857/" }, { "id": "PMID:27909074", "title": "Sulfatase activities are regulated by the interaction of the sulfatase-modifying factor 1 with SUMF2", "journal": "EMBO Rep", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27909074/" } ], "x": 3878.666666666667, "y": 1869.6346153846155, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614362", "label": "SUMF1 mediates the oxidation of cysteine to formylglycine, producing active arylsulfatases", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1614362", "summary": "SUMF1 mediates the oxidation of cysteine to formylglycine, producing active arylsulfatases is a Reactome release 97 curated reaction event. The source record connects ARS, NAD+ to active ARS, NADH, H2S in endoplasmic reticulum lumen.", "equation": "ARS + NAD+ → active ARS + NADH + H2S + H+", "inputs": [ { "node_id": "R-HSA-1614312", "reactome_id": "R-HSA-1614312", "label": "ARS", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-194653", "reactome_id": "R-ALL-194653", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-1614309", "reactome_id": "R-HSA-1614309", "label": "active ARS", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-194697", "reactome_id": "R-ALL-194697", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1663154", "reactome_id": "R-ALL-1663154", "label": "H2S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29919", "formula": "HS", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity of SUMF1 [endoplasmic reticulum lumen]", "activity_id": "1614322", "ec_numbers": [ "1" ], "accessions": [ "Q8NBK3" ] } ], "citations": [ { "id": "PMID:15657036", "title": "Molecular characterization of the human Calpha-formylglycine-generating enzyme", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15657036/" }, { "id": "PMID:12757706", "title": "The multiple sulfatase deficiency gene encodes an essential and limiting factor for the activity of sulfatases", "journal": "Cell", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12757706/" }, { "id": "PMID:14563551", "title": "The human SUMF1 gene, required for posttranslational sulfatase modification, defines a new gene family which is conserved from pro- to eukaryotes", "journal": "Gene", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14563551/" }, { "id": "PMID:12757705", "title": "Multiple sulfatase deficiency is caused by mutations in the gene encoding the human C(alpha)-formylglycine generating enzyme", "journal": "Cell", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12757705/" }, { "id": "PMID:8681943", "title": "The evolutionary conservation of a novel protein modification, the conversion of cysteine to serinesemialdehyde in arylsulfatase from Volvox carteri", "journal": "Eur J Biochem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8681943/" }, { "id": "PMID:9699462", "title": "A novel protein modification generating an aldehyde group in sulfatases: its role in catalysis and disease", "journal": "Bioessays", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9699462/" } ], "x": 3878.666666666667, "y": 1749.9423076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614524", "label": "PXLP-CBS tetramers condenses HCYS and L-Ser to form L-Cystathionine", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10113" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614524", "summary": "PXLP-CBS tetramers condenses HCYS and L-Ser to form L-Cystathionine is a Reactome release 97 curated reaction event. The source record connects HCYS, L-Ser to L-Cystathionine, H2O in cytosol and cross-references RHEA:10113.", "equation": "HCYS + L-Ser → L-Cystathionine + H2O", "inputs": [ { "node_id": "R-ALL-174369", "reactome_id": "R-ALL-174369", "label": "HCYS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58199", "formula": "C4H9NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1614518", "reactome_id": "R-ALL-1614518", "label": "L-Cystathionine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58161", "formula": "C7H14N2O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cystathionine beta-synthase activity of PXLP-K119-CBS tetramer [cytosol]", "activity_id": "1614644", "ec_numbers": [ "4.2.1.22" ], "accessions": [] } ], "citations": [ { "id": "PMID:11524006", "title": "Regulation of human cystathionine beta-synthase by S-adenosyl-L-methionine: evidence for two catalytically active conformations involving an autoinhibitory domain in the C-terminal region", "journal": "Biochemistry", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11524006/" }, { "id": "PMID:10052942", "title": "Binding of pyridoxal 5'-phosphate to the heme protein human cystathionine beta-synthase", "journal": "Biochemistry", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10052942/" } ], "x": 1808.6666666666667, "y": 2869.8888888888887, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614544", "label": "Sulfite is oxidized to sulfate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24601" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614544", "summary": "Sulfite is oxidized to sulfate is a Reactome release 97 curated reaction event. The source record connects sulfite(2-), O2, H2O to SO4(2-), H2O2 in mitochondrial matrix and cross-references RHEA:24601.", "equation": "sulfite(2-) + O2 + H2O → SO4(2-) + H2O2", "inputs": [ { "node_id": "R-ALL-1614538", "reactome_id": "R-ALL-1614538", "label": "sulfite(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17359", "formula": "O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1614584", "reactome_id": "R-ALL-1614584", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1614617", "reactome_id": "R-ALL-1614617", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfite oxidase activity of holo-SUOX [mitochondrial matrix]", "activity_id": "1614541", "ec_numbers": [ "1.8.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16475804", "title": "The G473D mutation impairs dimerization and catalysis in human sulfite oxidase", "journal": "Biochemistry", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16475804/" }, { "id": "PMID:17459792", "title": "Sulfite oxidizing enzymes", "journal": "Biochim Biophys Acta", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17459792/" } ], "x": 1808.6666666666667, "y": 2831.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614546", "label": "Sulfate is exported to the cytosol in exchange for dicarboxylate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial inner membrane · cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005829", "GO:0005759" ], "rhea_ids": [ "RHEA:71615" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614546", "summary": "Sulfate is exported to the cytosol in exchange for dicarboxylate is a Reactome release 97 curated reaction event. The source record connects MAL, SO4(2-) to MAL, SO4(2-) in mitochondrial inner membrane · cytosol · mitochondrial matrix and cross-references RHEA:71615.", "equation": "MAL + SO4(2-) → MAL + SO4(2-)", "inputs": [ { "node_id": "R-ALL-198498", "reactome_id": "R-ALL-198498", "label": "MAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1614584", "reactome_id": "R-ALL-1614584", "label": "SO4(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113544", "reactome_id": "R-ALL-113544", "label": "MAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174375", "reactome_id": "R-ALL-174375", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dicarboxylic acid transmembrane transporter activity of SLC25A10 [mitochondrial inner membrane]", "activity_id": "372844", "ec_numbers": null, "accessions": [ "Q9UBX3" ] } ], "citations": [ { "id": "PMID:10585886", "title": "Organization and sequence of the gene for the human mitochondrial dicarboxylate carrier: evolution of the carrier family", "journal": "Biochem J", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10585886/" }, { "id": "PMID:4441366", "title": "The transport of sulphate and sulphite in rat liver mitochondria", "journal": "Biochem J", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4441366/" } ], "x": 1251.3333333333333, "y": 2889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614567", "label": "Excess homocysteine yields homolanthionine and H2S", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:68653" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614567", "summary": "Excess homocysteine yields homolanthionine and H2S is a Reactome release 97 curated reaction event. The source record connects HCYS to HLAN, H2S, H+ in cytosol and cross-references RHEA:68653.", "equation": "2 HCYS → HLAN + H2S + H+", "inputs": [ { "node_id": "R-ALL-174369", "reactome_id": "R-ALL-174369", "label": "HCYS", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:58199", "formula": "C4H9NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1614594", "reactome_id": "R-ALL-1614594", "label": "HLAN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:178194", "formula": "C8H16N2O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1614532", "reactome_id": "R-ALL-1614532", "label": "H2S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29919", "formula": "HS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbon-sulfur lyase activity of PXLP-K212-CTH tetramer [cytosol]", "activity_id": "1614528", "ec_numbers": [ "4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:19261609", "title": "H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19261609/" }, { "id": "PMID:10212249", "title": "Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10212249/" } ], "x": 1530, "y": 2889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614583", "label": "PXLP-K212-CTH cleaves L-Cystathionine", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14006" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614583", "summary": "PXLP-K212-CTH cleaves L-Cystathionine is a Reactome release 97 curated reaction event. The source record connects L-Cystathionine, H2O to L-Cys, 2OBUTA, NH4+ in cytosol and cross-references RHEA:14006.", "equation": "L-Cystathionine + H2O → L-Cys + 2OBUTA + NH4+", "inputs": [ { "node_id": "R-ALL-1614518", "reactome_id": "R-ALL-1614518", "label": "L-Cystathionine", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58161", "formula": "C7H14N2O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-936714", "reactome_id": "R-ALL-936714", "label": "2OBUTA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16763", "formula": "C4H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cystathionine gamma-lyase activity of PXLP-K212-CTH tetramer [cytosol]", "activity_id": "1614601", "ec_numbers": [ "4.4.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:19261609", "title": "H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19261609/" }, { "id": "PMID:10212249", "title": "Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10212249/" } ], "x": 1251.3333333333333, "y": 2850.777777777778, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614591", "label": "Excess cysteine yields lanthionine and H2S", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:68649" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614591", "summary": "Excess cysteine yields lanthionine and H2S is a Reactome release 97 curated reaction event. The source record connects L-Cys to L-Lanthionine, H2S, H+ in cytosol and cross-references RHEA:68649.", "equation": "2 L-Cys → L-Lanthionine + H2S + H+", "inputs": [ { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1614582", "reactome_id": "R-ALL-1614582", "label": "L-Lanthionine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:178193", "formula": "C6H12N2O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1614532", "reactome_id": "R-ALL-1614532", "label": "H2S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29919", "formula": "HS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbon-sulfur lyase activity of PXLP-K212-CTH tetramer [cytosol]", "activity_id": "1614529", "ec_numbers": [ "4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:19261609", "title": "H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19261609/" }, { "id": "PMID:10212249", "title": "Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10212249/" } ], "x": 1808.6666666666667, "y": 2889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614605", "label": "Persulfide sulfur is dioxygenated", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1614605", "summary": "Persulfide sulfur is dioxygenated is a Reactome release 97 curated reaction event. The source record connects CysS-SQRDL(1-450), H2O, O2 to H+, sulfite(2-), SQRDL(1-450) in mitochondrial inner membrane · mitochondrial matrix.", "equation": "2 CysS-SQRDL(1-450) + 2 H2O + 2 O2 → 4 H+ + 2 sulfite(2-) + 2 SQRDL(1-450)", "inputs": [ { "node_id": "R-HSA-1614576", "reactome_id": "R-HSA-1614576", "label": "CysS-SQRDL(1-450)", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1614538", "reactome_id": "R-ALL-1614538", "label": "sulfite(2-)", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:17359", "formula": "O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-1614646", "reactome_id": "R-HSA-1614646", "label": "SQRDL(1-450)", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "sulfur dioxygenase activity of ETHE1:2Zn2+ [mitochondrial matrix]", "activity_id": "1614523", "ec_numbers": [ "1.13.11.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:19136963", "title": "Loss of ETHE1, a mitochondrial dioxygenase, causes fatal sulfide toxicity in ethylmalonic encephalopathy", "journal": "Nat Med", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19136963/" } ], "x": 1530, "y": 2984.5555555555557, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614614", "label": "Cysteine is degraded to pyruvate and H2S", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24932" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614614", "summary": "Cysteine is degraded to pyruvate and H2S is a Reactome release 97 curated reaction event. The source record connects H2O, L-Cys to H+, NH4+, PYR in cytosol and cross-references RHEA:24932.", "equation": "H2O + L-Cys → H+ + NH4+ + PYR + H2S", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1614532", "reactome_id": "R-ALL-1614532", "label": "H2S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29919", "formula": "HS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbon-sulfur lyase activity of PXLP-K212-CTH tetramer [cytosol]", "activity_id": "1614529", "ec_numbers": [ "4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:19261609", "title": "H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19261609/" }, { "id": "PMID:10212249", "title": "Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10212249/" } ], "x": 1530, "y": 2812.5555555555557, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614618", "label": "Persulfide sulfur is transferred onto sulfite", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1614618", "summary": "Persulfide sulfur is transferred onto sulfite is a Reactome release 97 curated reaction event. The source record connects CysS-SQRDL(1-450), sulfite(2-) to SQRDL(1-450), S2O3(2-) in mitochondrial inner membrane · mitochondrial matrix.", "equation": "2 CysS-SQRDL(1-450) + 2 sulfite(2-) → 2 SQRDL(1-450) + 2 S2O3(2-)", "inputs": [ { "node_id": "R-HSA-1614576", "reactome_id": "R-HSA-1614576", "label": "CysS-SQRDL(1-450)", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-1614538", "reactome_id": "R-ALL-1614538", "label": "sulfite(2-)", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:17359", "formula": "O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1614646", "reactome_id": "R-HSA-1614646", "label": "SQRDL(1-450)", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-1614595", "reactome_id": "R-ALL-1614595", "label": "S2O3(2-)", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:33542", "formula": "HO3S2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfurtransferase activity of TST [mitochondrial matrix]", "activity_id": "1614551", "ec_numbers": [ "2.8.1" ], "accessions": [ "Q16762" ] } ], "citations": [ { "id": "PMID:18494801", "title": "Three enzymatic activities catalyze the oxidation of sulfide to thiosulfate in mammalian and invertebrate mitochondria", "journal": "FEBS J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18494801/" } ], "x": 1251.3333333333333, "y": 3022.777777777778, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614631", "label": "Homocysteine is degraded to oxobutanoate and H2S", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14502" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614631", "summary": "Homocysteine is degraded to oxobutanoate and H2S is a Reactome release 97 curated reaction event. The source record connects HCYS, H2O to 2OBUTA, H2S, NH4+ in cytosol and cross-references RHEA:14502.", "equation": "HCYS + H2O → 2OBUTA + H2S + NH4+ + H+", "inputs": [ { "node_id": "R-ALL-174369", "reactome_id": "R-ALL-174369", "label": "HCYS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58199", "formula": "C4H9NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-936714", "reactome_id": "R-ALL-936714", "label": "2OBUTA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16763", "formula": "C4H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1614532", "reactome_id": "R-ALL-1614532", "label": "H2S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29919", "formula": "HS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "homocysteine desulfhydrase activity of PXLP-K212-CTH tetramer [cytosol]", "activity_id": "1614534", "ec_numbers": [ "4.4.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:19261609", "title": "H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19261609/" }, { "id": "PMID:10212249", "title": "Kinetics and inhibition of recombinant human cystathionine gamma-lyase. Toward the rational control of transsulfuration", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10212249/" } ], "x": 1808.6666666666667, "y": 2812.5555555555557, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614645", "label": "CDO1:Fe2+ oxidises L-Cys to 3-Sulfinoalanine", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20442" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614645", "summary": "CDO1:Fe2+ oxidises L-Cys to 3-Sulfinoalanine is a Reactome release 97 curated reaction event. The source record connects L-Cys, O2 to 3-Sulfinoalanine, H+ in cytosol and cross-references RHEA:20442.", "equation": "L-Cys + O2 → 3-Sulfinoalanine + H+", "inputs": [ { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1614519", "reactome_id": "R-ALL-1614519", "label": "3-Sulfinoalanine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61085", "formula": "C3H6NO4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cysteine dioxygenase activity of CDO1:Fe2+ [cytosol]", "activity_id": "1614590", "ec_numbers": [ "1.13.11.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:17135237", "title": "An insight into the mechanism of human cysteine dioxygenase. Key roles of the thioether-bonded tyrosine-cysteine cofactor", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17135237/" } ], "x": 1251.3333333333333, "y": 2908.1111111111113, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614654", "label": "BHMT tetramer transfers CH3 group from BET to HCYS to form DMGLY", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22337" ], "source_url": "https://reactome.org/content/detail/R-HSA-1614654", "summary": "BHMT tetramer transfers CH3 group from BET to HCYS to form DMGLY is a Reactome release 97 curated reaction event. The source record connects BET, HCYS to DMGLY, L-Met in cytosol and cross-references RHEA:22337.", "equation": "BET + HCYS → DMGLY + L-Met", "inputs": [ { "node_id": "R-ALL-352015", "reactome_id": "R-ALL-352015", "label": "BET", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17750", "formula": "C5H11NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174369", "reactome_id": "R-ALL-174369", "label": "HCYS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58199", "formula": "C4H9NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1614585", "reactome_id": "R-ALL-1614585", "label": "DMGLY", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58251", "formula": "C4H9NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174390", "reactome_id": "R-ALL-174390", "label": "L-Met", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57844", "formula": "C5H11NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "betaine-homocysteine S-methyltransferase activity of BHMT:Zn2+ tetramer [cytosol]", "activity_id": "1614655", "ec_numbers": [ "2.1.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:18457970", "title": "Human betaine-homocysteine methyltransferase (BHMT) and BHMT2: common gene sequence variation and functional characterization", "journal": "Mol Genet Metab", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18457970/" } ], "x": 1530, "y": 2908.1111111111113, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1614665", "label": "SQR oxidizes sulfide to bound persulfide", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial inner membrane · cytosol", "compartment_ids": [ "GO:0005743", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1614665", "summary": "SQR oxidizes sulfide to bound persulfide is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 2xHC-SQRDL(1-450), H2S, CoQ to CysS-SQRDL(1-450), QH2 in mitochondrial inner membrane · cytosol.", "equation": "2 2xHC-SQRDL(1-450) + 2 H2S + 2 CoQ + 4 H+ → 2 CysS-SQRDL(1-450) + 2 QH2", "inputs": [ { "node_id": "R-HSA-1614613", "reactome_id": "R-HSA-1614613", "label": "2xHC-SQRDL(1-450)", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-1614532", "reactome_id": "R-ALL-1614532", "label": "H2S", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:29919", "formula": "HS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73663", "reactome_id": "R-ALL-73663", "label": "CoQ", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:46245", "formula": "C59H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1614597", "reactome_id": "R-ALL-1614597", "label": "H+", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-1614576", "reactome_id": "R-HSA-1614576", "label": "CysS-SQRDL(1-450)", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-76633", "reactome_id": "R-ALL-76633", "label": "QH2", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:17976", "formula": "(C5H8)n.C9H12O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfide:quinone oxidoreductase activity of SQR:FAD [mitochondrial inner membrane]", "activity_id": "1614554", "ec_numbers": [ "1.8.5.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12832624", "title": "Single eubacterial origin of eukaryotic sulfide:quinone oxidoreductase, a mitochondrial enzyme conserved from the early evolution of eukaryotes during anoxic and sulfidic times", "journal": "Mol Biol Evol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12832624/" }, { "id": "PMID:18494801", "title": "Three enzymatic activities catalyze the oxidation of sulfide to thiosulfate in mammalian and invertebrate mitochondria", "journal": "FEBS J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18494801/" } ], "x": 1808.6666666666667, "y": 2984.5555555555557, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1630304", "label": "GALNS oligomer hydrolyses sulfate from Gal6S in keratan sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1630304", "summary": "GALNS oligomer hydrolyses sulfate from Gal6S in keratan sulfate is a Reactome release 97 curated reaction event. The source record connects H2O, Gal(S)-GlcNAc(S)-Gal-GlcNAc(S)-Gal to SO4(2-), Gal-GlcNAc(S)-Gal-GlcNAc(S)-Gal in lysosomal lumen.", "equation": "H2O + Gal(S)-GlcNAc(S)-Gal-GlcNAc(S)-Gal → SO4(2-) + Gal-GlcNAc(S)-Gal-GlcNAc(S)-Gal", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2105007", "reactome_id": "R-ALL-2105007", "label": "Gal(S)-GlcNAc(S)-Gal-GlcNAc(S)-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63846", "formula": "C34H58N2O35S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2104997", "reactome_id": "R-ALL-2104997", "label": "Gal-GlcNAc(S)-Gal-GlcNAc(S)-Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63850", "formula": "C34H58N2O31S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acetylgalactosamine-6-sulfatase activity of GALNS dimer [lysosomal lumen]", "activity_id": "1630318", "ec_numbers": [ "3.1.6.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:7213753", "title": "Purification and properties of human N-acetylgalactosamine-6-sulfate sulfatase", "journal": "Biochim Biophys Acta", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/7213753/" }, { "id": "PMID:1794986", "title": "N-acetylgalactosamine-6-sulfate sulfatase in human placenta: purification and characteristics", "journal": "J Biochem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1794986/" }, { "id": "PMID:12888274", "title": "Crystal structure of a covalent intermediate of endogenous human arylsulfatase A", "journal": "J Inorg Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12888274/" }, { "id": "PMID:9521684", "title": "Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis", "journal": "Biochemistry", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9521684/" }, { "id": "PMID:1522213", "title": "Mucopolysaccharidosis type IVA. N-acetylgalactosamine-6-sulfate sulfatase exonic point mutations in classical Morquio and mild cases", "journal": "J Clin Invest", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1522213/" }, { "id": "PMID:22940367", "title": "The structure of human GALNS reveals the molecular basis for mucopolysaccharidosis IV A", "journal": "J Mol Biol", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22940367/" } ], "x": 2381.3333333333335, "y": 924.7121212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1630306", "label": "GLB1 hydrolyses a glycosaminoglycan", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1630306", "summary": "GLB1 hydrolyses a glycosaminoglycan is a Reactome release 97 curated reaction event. The source record connects H2O, Gal-GlcNAc(S)-Gal-GlcNAc(S)-Gal to Gal, GlcNAc(S)-Gal-GlcNAc(S)-Gal in lysosomal lumen.", "equation": "H2O + Gal-GlcNAc(S)-Gal-GlcNAc(S)-Gal → Gal + GlcNAc(S)-Gal-GlcNAc(S)-Gal", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2104997", "reactome_id": "R-ALL-2104997", "label": "Gal-GlcNAc(S)-Gal-GlcNAc(S)-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63850", "formula": "C34H58N2O31S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605637", "reactome_id": "R-ALL-1605637", "label": "Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2104995", "reactome_id": "R-ALL-2104995", "label": "GlcNAc(S)-Gal-GlcNAc(S)-Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63851", "formula": "C28H48N2O27S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-galactosidase activity of BGAL [lysosomal lumen]", "activity_id": "1605789", "ec_numbers": [ "3.2.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:5822067", "title": "Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase", "journal": "Biochem J", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5822067/" }, { "id": "PMID:1907800", "title": "Human beta-galactosidase gene mutations in GM1-gangliosidosis: a common mutation among Japanese adult/chronic cases", "journal": "Am J Hum Genet", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1907800/" }, { "id": "PMID:1928092", "title": "Human beta-galactosidase gene mutations in morquio B disease", "journal": "Am J Hum Genet", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1928092/" } ], "x": 2660, "y": 924.7121212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163213", "label": "Transfer of electrons through the succinate dehydrogenase complex", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [ "RHEA:13714" ], "source_url": "https://reactome.org/content/detail/R-HSA-163213", "summary": "Transfer of electrons through the succinate dehydrogenase complex is a Reactome release 97 curated reaction event. The source record connects CoQ, SUCCA to FUMA, CoQH2 in mitochondrial inner membrane and cross-references RHEA:13714.", "equation": "CoQ + SUCCA → FUMA + CoQH2", "inputs": [ { "node_id": "R-ALL-73663", "reactome_id": "R-ALL-73663", "label": "CoQ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46245", "formula": "C59H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113536", "reactome_id": "R-ALL-113536", "label": "SUCCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30031", "formula": "C4H4O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113588", "reactome_id": "R-ALL-113588", "label": "FUMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29806", "formula": "C4H2O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162198", "reactome_id": "R-ALL-2162198", "label": "CoQH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "succinate dehydrogenase (quinone) activity of SDH complex [mitochondrial inner membrane]", "activity_id": "70993", "ec_numbers": [ "1.3.5.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:23747254", "title": "The mitochondrial chaperone TRAP1 promotes neoplastic growth by inhibiting succinate dehydrogenase", "journal": "Cell Metab.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23747254/" }, { "id": "PMID:23564345", "title": "Molecular chaperone TRAP1 regulates a metabolic switch between mitochondrial respiration and aerobic glycolysis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23564345/" }, { "id": "PMID:25564869", "title": "Inhibition of succinate dehydrogenase by the mitochondrial chaperone TRAP1 has anti-oxidant and anti-apoptotic effects on tumor cells", "journal": "Oncotarget", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25564869/" } ], "x": 4451.333333333333, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163214", "label": "Electron transfer from reduced cytochrome c to molecular oxygen", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial intermembrane space · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005758", "GO:0005759" ], "rhea_ids": [ "RHEA:11437" ], "source_url": "https://reactome.org/content/detail/R-HSA-163214", "summary": "Electron transfer from reduced cytochrome c to molecular oxygen is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects O2, H+, Cytochrome c (reduced) to H2O, H+, Cytochrome c (oxidised) in mitochondrial inner membrane · mitochondrial intermembrane space · mitochondrial matrix and cross-references RHEA:11437.", "equation": "O2 + 12 H+ + 4 Cytochrome c (reduced) → 2 H2O + 8 H+ + 4 Cytochrome c (oxidised)", "inputs": [ { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 12, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-352609", "reactome_id": "R-HSA-352609", "label": "Cytochrome c (reduced)", "role": "input", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-163953", "reactome_id": "R-ALL-163953", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-352607", "reactome_id": "R-HSA-352607", "label": "Cytochrome c (oxidised)", "role": "output", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "cytochrome-c oxidase activity of Cytochrome c oxidase [mitochondrial inner membrane]", "activity_id": "164322", "ec_numbers": [ "7.1.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:7979252", "title": "Energy transduction by cytochrome complexes in mitochondrial and bacterial", "journal": "Annu Rev Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7979252/" }, { "id": "PMID:11340051", "title": "Structures and proton-pumping strategies of mitochondrial respiratory", "journal": "Annu Rev Biophys Biomol Struct", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11340051/" }, { "id": "PMID:15223", "title": "Proton pump coupled to cytochrome c oxidase in mitochondria", "journal": "Nature", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/15223/" }, { "id": "PMID:16760263", "title": "Assembly of mitochondrial cytochrome c-oxidase, a complicated and highly regulated cellular process", "journal": "Am. J. Physiol., Cell Physiol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16760263/" }, { "id": "PMID:21958598", "title": "Biogenesis and assembly of eukaryotic cytochrome c oxidase catalytic core", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/21958598/" }, { "id": "PMID:17298220", "title": "Biogenesis of eukaryotic cytochrome c oxidase", "journal": "Physiol Res", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17298220/" } ], "x": 4730, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-163217", "label": "Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [ "RHEA:29092" ], "source_url": "https://reactome.org/content/detail/R-HSA-163217", "summary": "Complex I oxidises NADH to NAD+, reduces CoQ to CoQH2 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects NADH, H+, CoQ to NAD+, H+, CoQH2 in mitochondrial inner membrane and cross-references RHEA:29092.", "equation": "NADH + 5 H+ + CoQ → NAD+ + 4 H+ + CoQH2", "inputs": [ { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 5, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73663", "reactome_id": "R-ALL-73663", "label": "CoQ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46245", "formula": "C59H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-163953", "reactome_id": "R-ALL-163953", "label": "H+", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2162198", "reactome_id": "R-ALL-2162198", "label": "CoQH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "NADH dehydrogenase (ubiquinone) activity of Complex I [mitochondrial inner membrane]", "activity_id": "164321", "ec_numbers": [ "7.1.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9878551", "title": "cDNA of eight nuclear encoded subunits of NADH:ubiquinone oxidoreductase:", "journal": "Biochem Biophys Res Commun", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9878551/" }, { "id": "PMID:12837546", "title": "The nuclear encoded subunits of complex I from bovine heart mitochondria", "journal": "Biochim Biophys Acta", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12837546/" }, { "id": "PMID:15250827", "title": "Structural organization of mitochondrial human complex I: role of the ND4", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15250827/" }, { "id": "PMID:14741580", "title": "The gross structure of the respiratory complex I: a Lego System", "journal": "Biochim Biophys Acta", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14741580/" }, { "id": "PMID:11695836", "title": "Human NADH:ubiquinone oxidoreductase", "journal": "J Bioenerg Biomembr", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11695836/" }, { "id": "PMID:12231006", "title": "The energy-transducing NADH: quinone oxidoreductase, complex I", "journal": "Mol Aspects Med", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12231006/" } ], "x": 5008.666666666667, "y": 780.9, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-163402", "label": "diacylglycerol + H2O -\u003e 2-acylglycerol + fatty acid", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · lipid droplet", "compartment_ids": [ "GO:0005829", "GO:0005811" ], "rhea_ids": [ "RHEA:33276" ], "source_url": "https://reactome.org/content/detail/R-HSA-163402", "summary": "diacylglycerol + H2O -\u003e 2-acylglycerol + fatty acid is a Reactome release 97 curated reaction event. The source record connects DAG, H2O to 2-acylglycerol, LCFA(-), H+ in cytosol · lipid droplet and cross-references RHEA:33276.", "equation": "DAG + H2O → 2-acylglycerol + LCFA(-) + H+", "inputs": [ { "node_id": "R-ALL-163414", "reactome_id": "R-ALL-163414", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-163580", "reactome_id": "R-ALL-163580", "label": "2-acylglycerol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17389", "formula": "C4H7O4R", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8876730", "reactome_id": "R-ALL-8876730", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "lipase activity of phosphorylated HSL dimer:FABP4 complex [lipid droplet]", "activity_id": "163604", "ec_numbers": [ "3.1.1" ], "accessions": [] } ], "citations": [], "x": 3321.3333333333335, "y": 2084.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163416", "label": "hormone-sensitive lipase (HSL) + 2 ATP -\u003e phosphorylated HSL + 2 ADP", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163416", "summary": "hormone-sensitive lipase (HSL) + 2 ATP -\u003e phosphorylated HSL + 2 ADP is a Reactome release 97 curated reaction event. The source record connects LIPE, ATP to p-S950,S951-LIPE, ADP in cytosol.", "equation": "LIPE + 2 ATP → p-S950,S951-LIPE + 2 ADP", "inputs": [ { "node_id": "R-HSA-162503", "reactome_id": "R-HSA-162503", "label": "LIPE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-162472", "reactome_id": "R-HSA-162472", "label": "p-S950,S951-LIPE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein serine/threonine kinase activity of PKA catalytic subunit [cytosol]", "activity_id": "163605", "ec_numbers": [ "2.7.11.1" ], "accessions": [] } ], "citations": [], "x": 3878.666666666667, "y": 2230.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163418", "label": "perilipin + 2 ATP -\u003e phosphorylated perilipin + 2 ADP", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · lipid droplet", "compartment_ids": [ "GO:0005829", "GO:0005811" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163418", "summary": "perilipin + 2 ATP -\u003e phosphorylated perilipin + 2 ADP is a Reactome release 97 curated reaction event. The source record connects PLIN1, ATP to p-S81,S277-PLIN1, ADP in cytosol · lipid droplet.", "equation": "PLIN1 + 2 ATP → p-S81,S277-PLIN1 + 2 ADP", "inputs": [ { "node_id": "R-HSA-163460", "reactome_id": "R-HSA-163460", "label": "PLIN1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-163561", "reactome_id": "R-HSA-163561", "label": "p-S81,S277-PLIN1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein serine/threonine kinase activity of PKA catalytic subunit [cytosol]", "activity_id": "163605", "ec_numbers": [ "2.7.11.1" ], "accessions": [] } ], "citations": [], "x": 3321.3333333333335, "y": 2279.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163432", "label": "cholesterol ester + H2O -\u003e cholesterol + fatty acid", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · lipid droplet", "compartment_ids": [ "GO:0005829", "GO:0005811" ], "rhea_ids": [ "RHEA:36404" ], "source_url": "https://reactome.org/content/detail/R-HSA-163432", "summary": "cholesterol ester + H2O -\u003e cholesterol + fatty acid is a Reactome release 97 curated reaction event. The source record connects CHEST, H2O to CHOL, LCFA(-), H+ in cytosol · lipid droplet and cross-references RHEA:36404.", "equation": "CHEST + H2O → CHOL + LCFA(-) + H+", "inputs": [ { "node_id": "R-ALL-163579", "reactome_id": "R-ALL-163579", "label": "CHEST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17002", "formula": "C28H45O2R", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-163586", "reactome_id": "R-ALL-163586", "label": "CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8876730", "reactome_id": "R-ALL-8876730", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "lipase activity of phosphorylated HSL dimer:FABP4 complex [lipid droplet]", "activity_id": "163604", "ec_numbers": [ "3.1.1" ], "accessions": [] } ], "citations": [], "x": 3600, "y": 2084.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163489", "label": "phosphorylated HSL + H2O -\u003e HSL + orthophosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163489", "summary": "phosphorylated HSL + H2O -\u003e HSL + orthophosphate is a Reactome release 97 curated reaction event. The source record connects p-S950,S951-LIPE, H2O to LIPE, Pi in cytosol.", "equation": "p-S950,S951-LIPE + H2O → LIPE + Pi", "inputs": [ { "node_id": "R-HSA-162472", "reactome_id": "R-HSA-162472", "label": "p-S950,S951-LIPE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-162503", "reactome_id": "R-HSA-162503", "label": "LIPE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [], "x": 3600, "y": 2328.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163539", "label": "perilipin:ABHD5 complex -\u003e perilipin + ABHD5", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · lipid droplet", "compartment_ids": [ "GO:0005829", "GO:0005811" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163539", "summary": "perilipin:ABHD5 complex -\u003e perilipin + ABHD5 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects perilipin:ABHD5 complex to ABHD5, PLIN1 in cytosol · lipid droplet.", "equation": "perilipin:ABHD5 complex → ABHD5 + PLIN1", "inputs": [ { "node_id": "R-HSA-163495", "reactome_id": "R-HSA-163495", "label": "perilipin:ABHD5 complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-163531", "reactome_id": "R-HSA-163531", "label": "ABHD5", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-163460", "reactome_id": "R-HSA-163460", "label": "PLIN1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [], "x": 3878.666666666667, "y": 2328.0625, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-163549", "label": "phosphorylated HSL dimer + FABP4 -\u003e phosphorylated HSL dimer:FABP4 complex", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "lipid droplet", "compartment_ids": [ "GO:0005811" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163549", "summary": "phosphorylated HSL dimer + FABP4 -\u003e phosphorylated HSL dimer:FABP4 complex is a Reactome release 97 curated reaction event. The source record connects FABP4, phosphorylated HSL dimer to phosphorylated HSL dimer:FABP4 complex in lipid droplet.", "equation": "FABP4 + phosphorylated HSL dimer → phosphorylated HSL dimer:FABP4 complex", "inputs": [ { "node_id": "R-HSA-163428", "reactome_id": "R-HSA-163428", "label": "FABP4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-163435", "reactome_id": "R-HSA-163435", "label": "phosphorylated HSL dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-163535", "reactome_id": "R-HSA-163535", "label": "phosphorylated HSL dimer:FABP4 complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "lipase activator activity of FABP4 [lipid droplet]", "activity_id": "9958495", "ec_numbers": null, "accessions": [ "P15090" ] } ], "citations": [ { "id": "PMID:15456755", "title": "Physical association between the adipocyte fatty acid-binding protein and hormone-sensitive lipase: a fluorescence resonance energy transfer analysis", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15456755/" } ], "x": 3321.3333333333335, "y": 2328.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163551", "label": "triacylglycerol + H2O -\u003e diacylglycerol + fatty acid", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · lipid droplet", "compartment_ids": [ "GO:0005829", "GO:0005811" ], "rhea_ids": [ "RHEA:12045" ], "source_url": "https://reactome.org/content/detail/R-HSA-163551", "summary": "triacylglycerol + H2O -\u003e diacylglycerol + fatty acid is a Reactome release 97 curated reaction event. The source record connects TAG, H2O to DAG, LCFA(-), H+ in cytosol · lipid droplet and cross-references RHEA:12045.", "equation": "TAG + H2O → DAG + LCFA(-) + H+", "inputs": [ { "node_id": "R-ALL-163438", "reactome_id": "R-ALL-163438", "label": "TAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-163414", "reactome_id": "R-ALL-163414", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8876730", "reactome_id": "R-ALL-8876730", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "triacylglycerol lipase activity of phosphorylated HSL dimer:FABP4 complex [lipid droplet]", "activity_id": "163447", "ec_numbers": [ "3.1.1.3" ], "accessions": [] } ], "citations": [], "x": 3878.666666666667, "y": 2084.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163554", "label": "Phosphorylated HSL dimer translocates from the cytosol to the lipid particle", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · lipid droplet", "compartment_ids": [ "GO:0005829", "GO:0005811" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163554", "summary": "Phosphorylated HSL dimer translocates from the cytosol to the lipid particle is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects phosphorylated HSL dimer to phosphorylated HSL dimer in cytosol · lipid droplet.", "equation": "phosphorylated HSL dimer → phosphorylated HSL dimer", "inputs": [ { "node_id": "R-HSA-163513", "reactome_id": "R-HSA-163513", "label": "phosphorylated HSL dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-163435", "reactome_id": "R-HSA-163435", "label": "phosphorylated HSL dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12832420", "title": "Mutational analysis of the hormone-sensitive lipase translocation reaction in adipocytes", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12832420/" }, { "id": "PMID:12810697", "title": "Perilipin A is essential for the translocation of hormone-sensitive lipase during lipolytic activation", "journal": "J Cell Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12810697/" }, { "id": "PMID:10671541", "title": "Translocation of hormone-sensitive lipase and perilipin upon lipolytic stimulation of rat adipocytes", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10671541/" }, { "id": "PMID:40221426", "title": "Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets", "journal": "Nat Commun", "year": 2025, "url": "https://pubmed.ncbi.nlm.nih.gov/40221426/" } ], "x": 3600, "y": 2376.6875, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-163568", "label": "phosphorylated perilipin + H2O -\u003e perilipin + orthophosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · lipid droplet", "compartment_ids": [ "GO:0005829", "GO:0005811" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163568", "summary": "phosphorylated perilipin + H2O -\u003e perilipin + orthophosphate is a Reactome release 97 curated reaction event. The source record connects p-S81,S277-PLIN1, H2O to PLIN1, Pi in cytosol · lipid droplet.", "equation": "p-S81,S277-PLIN1 + H2O → PLIN1 + Pi", "inputs": [ { "node_id": "R-HSA-163561", "reactome_id": "R-HSA-163561", "label": "p-S81,S277-PLIN1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-163460", "reactome_id": "R-HSA-163460", "label": "PLIN1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lipid droplet", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "protein serine/threonine phosphatase activity of PP1 catalytic subunit [cytosol]", "activity_id": "163421", "ec_numbers": [ "3.1.3.16" ], "accessions": [] } ], "citations": [], "x": 3600, "y": 2279.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163595", "label": "2-acylglycerol + H2O -\u003e glycerol + fatty acid", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · lipid droplet", "compartment_ids": [ "GO:0005829", "GO:0005811" ], "rhea_ids": [ "RHEA:44689" ], "source_url": "https://reactome.org/content/detail/R-HSA-163595", "summary": "2-acylglycerol + H2O -\u003e glycerol + fatty acid is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 2-acylglycerol, H2O to Glycerol, LCFA(-), H+ in cytosol · lipid droplet and cross-references RHEA:44689.", "equation": "2-acylglycerol + H2O → Glycerol + LCFA(-) + H+", "inputs": [ { "node_id": "R-ALL-163580", "reactome_id": "R-ALL-163580", "label": "2-acylglycerol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17389", "formula": "C4H7O4R", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76116", "reactome_id": "R-ALL-76116", "label": "Glycerol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17754", "formula": "C3H8O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8876730", "reactome_id": "R-ALL-8876730", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monoacylglycerol lipase activity of MGLL [lipid droplet]", "activity_id": "163500", "ec_numbers": [ "3.1.1.23" ], "accessions": [ "Q99685" ] } ], "citations": [], "x": 3321.3333333333335, "y": 2133.5625, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-163602", "label": "2 phosphorylated HSL monomers -\u003e phosphorylated HSL dimer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163602", "summary": "2 phosphorylated HSL monomers -\u003e phosphorylated HSL dimer is a Reactome release 97 curated reaction event. The source record connects p-S950,S951-LIPE to phosphorylated HSL dimer in cytosol.", "equation": "2 p-S950,S951-LIPE → phosphorylated HSL dimer", "inputs": [ { "node_id": "R-HSA-162472", "reactome_id": "R-HSA-162472", "label": "p-S950,S951-LIPE", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-163513", "reactome_id": "R-HSA-163513", "label": "phosphorylated HSL dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [], "x": 3321.3333333333335, "y": 2376.6875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163741", "label": "D-glyceraldehyde 3-phosphate + sedoheptulose 7-phosphate xylulose 5-phosphate+ribose 5-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10509" ], "source_url": "https://reactome.org/content/detail/R-HSA-163741", "summary": "D-glyceraldehyde 3-phosphate + sedoheptulose 7-phosphate xylulose 5-phosphate+ribose 5-phosphate is a Reactome release 97 curated reaction event. The source record connects GA3P, SH7P to R5P, XY5P in cytosol and cross-references RHEA:10509.", "equation": "GA3P + SH7P → R5P + XY5P", "inputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29882", "reactome_id": "R-ALL-29882", "label": "SH7P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57483", "formula": "C7H13O10P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29790", "reactome_id": "R-ALL-29790", "label": "XY5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57737", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transketolase activity of TKT dimer [cytosol]", "activity_id": "71323", "ec_numbers": [ "2.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9357955", "title": "Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation", "journal": "Biochim Biophys Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9357955/" } ], "x": 311.33333333333337, "y": 1047.642857142857, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163750", "label": "PP2A complex dephosphorylates pPFKFB1", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20630" ], "source_url": "https://reactome.org/content/detail/R-HSA-163750", "summary": "PP2A complex dephosphorylates pPFKFB1 is a Reactome release 97 curated reaction event. The source record connects p-PFKFB1 dimer, H2O to PFKFB1 dimer, Pi in cytosol and cross-references RHEA:20630.", "equation": "p-PFKFB1 dimer + 2 H2O → PFKFB1 dimer + 2 Pi", "inputs": [ { "node_id": "R-HSA-163745", "reactome_id": "R-HSA-163745", "label": "p-PFKFB1 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-71786", "reactome_id": "R-HSA-71786", "label": "PFKFB1 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "protein serine/threonine phosphatase activity of PP2A-ABdeltaC complex [cytosol]", "activity_id": "163684", "ec_numbers": [ "3.1.3.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:12721358", "title": "A humble hexose monophosphate pathway metabolite regulates short- and long-term control of lipogenesis", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12721358/" } ], "x": 868.6666666666667, "y": 530.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163751", "label": "D-glyceraldehyde 3-phosphate + D-fructose 6-phosphate xylulose 5-phosphate + D-erythrose 4-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27628" ], "source_url": "https://reactome.org/content/detail/R-HSA-163751", "summary": "D-glyceraldehyde 3-phosphate + D-fructose 6-phosphate xylulose 5-phosphate + D-erythrose 4-phosphate is a Reactome release 97 curated reaction event. The source record connects GA3P, Fru(6)P to XY5P, E4P in cytosol and cross-references RHEA:27628.", "equation": "GA3P + Fru(6)P → XY5P + E4P", "inputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29790", "reactome_id": "R-ALL-29790", "label": "XY5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57737", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29878", "reactome_id": "R-ALL-29878", "label": "E4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16897", "formula": "C4H7O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transketolase activity of TKT dimer [cytosol]", "activity_id": "71323", "ec_numbers": [ "2.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9357955", "title": "Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation", "journal": "Biochim Biophys Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9357955/" } ], "x": 590, "y": 825.3571428571429, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163756", "label": "Formation of fatty acid synthase (FAS) dimer", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-163756", "summary": "Formation of fatty acid synthase (FAS) dimer is a Reactome release 97 curated reaction event. The source record connects FASN to FASN dimer in cytosol.", "equation": "2 FASN → FASN dimer", "inputs": [ { "node_id": "R-HSA-54659", "reactome_id": "R-HSA-54659", "label": "FASN", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-77380", "reactome_id": "R-HSA-77380", "label": "FASN dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "fatty acid synthase activity of FASN [cytosol]", "activity_id": "9865848", "ec_numbers": [ "2.3.1.85" ], "accessions": [ "P49327" ] } ], "citations": [ { "id": "PMID:18455495", "title": "Differential activation of recombinant human acetyl-CoA carboxylases 1 and 2 by citrate", "journal": "Arch Biochem Biophys", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18455495/" } ], "x": 868.6666666666667, "y": 1739.701754385965, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163764", "label": "D-fructose 6-phosphate + D-erythrose 4-phosphate sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17055" ], "source_url": "https://reactome.org/content/detail/R-HSA-163764", "summary": "D-fructose 6-phosphate + D-erythrose 4-phosphate sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate is a Reactome release 97 curated reaction event. The source record connects Fru(6)P, E4P to SH7P, GA3P in cytosol and cross-references RHEA:17055.", "equation": "Fru(6)P + E4P → SH7P + GA3P", "inputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29878", "reactome_id": "R-ALL-29878", "label": "E4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16897", "formula": "C4H7O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29882", "reactome_id": "R-ALL-29882", "label": "SH7P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57483", "formula": "C7H13O10P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transaldolase activity of TALDO1 dimer [cytosol]", "activity_id": "71333", "ec_numbers": [ "2.2.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8300619", "title": "Cloning and expression of the human gene for transaldolase. A novel highly repetitive element constitutes an integral part of the coding sequence", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8300619/" }, { "id": "PMID:10869557", "title": "The three-dimensional structure of human transaldolase", "journal": "FEBS Lett.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10869557/" }, { "id": "PMID:11283793", "title": "Transaldolase deficiency: liver cirrhosis associated with a new inborn error in the pentose phosphate pathway.", "journal": "Am J Hum Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11283793/" } ], "x": 868.6666666666667, "y": 825.3571428571429, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-163773", "label": "PKA tetramer:cAMP phosphorylates PF2K-Pase", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17990" ], "source_url": "https://reactome.org/content/detail/R-HSA-163773", "summary": "PKA tetramer:cAMP phosphorylates PF2K-Pase is a Reactome release 97 curated reaction event. The source record connects PFKFB1 dimer, ATP to p-PFKFB1 dimer, ADP, H+ in cytosol and cross-references RHEA:17990.", "equation": "PFKFB1 dimer + 2 ATP → p-PFKFB1 dimer + 2 ADP + 2 H+", "inputs": [ { "node_id": "R-HSA-71786", "reactome_id": "R-HSA-71786", "label": "PFKFB1 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-163745", "reactome_id": "R-HSA-163745", "label": "p-PFKFB1 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cAMP-dependent protein kinase activity of PKA catalytic subunit [cytosol]", "activity_id": "163671", "ec_numbers": [ "2.7.11.11" ], "accessions": [] } ], "citations": [], "x": 590, "y": 530.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1638032", "label": "GNS:Ca2+ cleaves sulfate from GlcNAc-6-sulfate units of keratan sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1638032", "summary": "GNS:Ca2+ cleaves sulfate from GlcNAc-6-sulfate units of keratan sulfate is a Reactome release 97 curated reaction event. The source record connects H2O, GlcNAc(S)-Gal-GlcNAc(S)-Gal to SO4(2-), GlcNAc-Gal-GlcNAc(S)-Gal in lysosomal lumen.", "equation": "H2O + GlcNAc(S)-Gal-GlcNAc(S)-Gal → SO4(2-) + GlcNAc-Gal-GlcNAc(S)-Gal", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2104995", "reactome_id": "R-ALL-2104995", "label": "GlcNAc(S)-Gal-GlcNAc(S)-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63851", "formula": "C28H48N2O27S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2105012", "reactome_id": "R-ALL-2105012", "label": "GlcNAc-Gal-GlcNAc(S)-Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63852", "formula": "C28H48N2O24S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acetylglucosamine-6-sulfatase activity of GNS:Ca2+ [lysosomal lumen]", "activity_id": "1638056", "ec_numbers": [ "3.1.6.14" ], "accessions": [] } ], "citations": [ { "id": "PMID:1463457", "title": "A cDNA clone for human glucosamine-6-sulphatase reveals differences between arylsulphatases and non-arylsulphatases", "journal": "Biochem J", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1463457/" }, { "id": "PMID:3196333", "title": "Human glucosamine-6-sulfatase cDNA reveals homology with steroid sulfatase", "journal": "Biochem Biophys Res Commun", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3196333/" }, { "id": "PMID:20232353", "title": "Mucopolysaccharidosis type IIID: 12 new patients and 15 novel mutations", "journal": "Hum Mutat", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20232353/" } ], "x": 2938.666666666667, "y": 924.7121212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1638053", "label": "HEXA cleaves the terminal GlcNAc from keratan sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1638053", "summary": "HEXA cleaves the terminal GlcNAc from keratan sulfate is a Reactome release 97 curated reaction event. The source record connects H2O, GlcNAc-Gal-GlcNAc(S)-Gal to Gal-GlcNAc(S)-Gal, GlcNAc in lysosomal lumen.", "equation": "H2O + GlcNAc-Gal-GlcNAc(S)-Gal → Gal-GlcNAc(S)-Gal + GlcNAc", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2105012", "reactome_id": "R-ALL-2105012", "label": "GlcNAc-Gal-GlcNAc(S)-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63852", "formula": "C28H48N2O24S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2105010", "reactome_id": "R-ALL-2105010", "label": "Gal-GlcNAc(S)-Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63853", "formula": "C20H35NO19S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1678743", "reactome_id": "R-ALL-1678743", "label": "GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17411", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXA [lysosomal lumen]", "activity_id": "1605773", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:2971395", "title": "Oligosaccharide structure and amino acid sequence of the major glycopeptides of mature human beta-hexosaminidase", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2971395/" } ], "x": 2381.3333333333335, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1638104", "label": "UGCG transfers glucose to ceramide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:12088" ], "source_url": "https://reactome.org/content/detail/R-HSA-1638104", "summary": "UGCG transfers glucose to ceramide is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, CERA-d18 to GlcCer, UDP, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:12088.", "equation": "UDP-Glc + CERA-d18 → GlcCer + UDP + H+", "inputs": [ { "node_id": "R-ALL-744227", "reactome_id": "R-ALL-744227", "label": "UDP-Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9844975", "reactome_id": "R-ALL-9844975", "label": "CERA-d18", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:52639", "formula": "C19H36NO3R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1660674", "reactome_id": "R-ALL-1660674", "label": "GlcCer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:22801", "formula": "C25H46NO8R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ceramide glucosyltransferase activity of UGCG [Golgi membrane]", "activity_id": "1638095", "ec_numbers": [ "2.4.1.80" ], "accessions": [ "Q16739" ] } ], "citations": [ { "id": "PMID:8643456", "title": "Expression cloning of a cDNA for human ceramide glucosyltransferase that catalyzes the first glycosylation step of glycosphingolipid synthesis", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8643456/" }, { "id": "PMID:15799713", "title": "The reconstituted P-glycoprotein multidrug transporter is a flippase for glucosylceramide and other simple glycosphingolipids", "journal": "Biochem J", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15799713/" }, { "id": "PMID:14662772", "title": "Role of multiple drug resistance protein 1 in neutral but not acidic glycosphingolipid biosynthesis", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14662772/" }, { "id": "PMID:23290777", "title": "Ceramide glycosylation catalyzed by glucosylceramide synthase and cancer drug resistance", "journal": "Adv. Cancer Res.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23290777/" } ], "x": 3878.666666666667, "y": 1555.4423076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1638845", "label": "CERK phosphorylates CERA to form C1P", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1638845", "summary": "CERK phosphorylates CERA to form C1P is a Reactome release 97 curated reaction event. The source record connects ATP, CERA to ADP, C1P, H+ in plasma membrane · cytosol.", "equation": "ATP + CERA → ADP + C1P + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-429807", "reactome_id": "R-ALL-429807", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1638840", "reactome_id": "R-ALL-1638840", "label": "C1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16197", "formula": "C19H37NO6PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ceramide kinase activity of CERK [plasma membrane]", "activity_id": "1638839", "ec_numbers": [ "2.7.1.138" ], "accessions": [ "Q8TCT0" ] } ], "citations": [ { "id": "PMID:11956206", "title": "Ceramide kinase, a novel lipid kinase. Molecular cloning and functional characterization", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11956206/" }, { "id": "PMID:21111813", "title": "Ceramide kinase: the first decade", "journal": "Cell Signal", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21111813/" }, { "id": "PMID:29269995", "title": "The Role of Sphingosine-1-Phosphate and Ceramide-1-Phosphate in Inflammation and Cancer", "journal": "Mediators Inflamm", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/29269995/" } ], "x": 3600, "y": 1690.0961538461538, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1640164", "label": "ENPP7 hydrolyzes sphingomyelin", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1640164", "summary": "ENPP7 hydrolyzes sphingomyelin is a Reactome release 97 curated reaction event. The source record connects H2O, SPHM d18:1 to CERA, ChoP, H+ in plasma membrane · cytosol.", "equation": "H2O + SPHM d18:1 → CERA + ChoP + H+", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-429776", "reactome_id": "R-ALL-429776", "label": "SPHM d18:1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17636", "formula": "C24H48N2O6PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-429807", "reactome_id": "R-ALL-429807", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1610735", "reactome_id": "R-ALL-1610735", "label": "ChoP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:295975", "formula": "C5H13NO4P", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sphingomyelin phosphodiesterase activity of ENPP7 [plasma membrane]", "activity_id": "1640163", "ec_numbers": [ "3.1.4.12" ], "accessions": [ "Q6UWV6" ] } ], "citations": [ { "id": "PMID:12885774", "title": "Identification of human intestinal alkaline sphingomyelinase as a novel ecto-enzyme related to the nucleotide phosphodiesterase family", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12885774/" }, { "id": "PMID:15458386", "title": "Functional studies of human intestinal alkaline sphingomyelinase by deglycosylation and mutagenesis", "journal": "Biochem J", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15458386/" } ], "x": 3878.666666666667, "y": 1690.0961538461538, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-164651", "label": "Electron transfer from ubiquinol to cytochrome c of complex III", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [ "RHEA:11485" ], "source_url": "https://reactome.org/content/detail/R-HSA-164651", "summary": "Electron transfer from ubiquinol to cytochrome c of complex III is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CoQ, H+, Cytochrome c (oxidised) to CoQ, CoQH2, H+ in mitochondrial inner membrane and cross-references RHEA:11485.", "equation": "CoQ + 2 H+ + 2 Cytochrome c (oxidised) + 2 CoQH2 → 2 CoQ + CoQH2 + 4 H+ + 2 Cytochrome c (reduced)", "inputs": [ { "node_id": "R-ALL-73663", "reactome_id": "R-ALL-73663", "label": "CoQ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46245", "formula": "C59H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-352607", "reactome_id": "R-HSA-352607", "label": "Cytochrome c (oxidised)", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-2162198", "reactome_id": "R-ALL-2162198", "label": "CoQH2", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73663", "reactome_id": "R-ALL-73663", "label": "CoQ", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:46245", "formula": "C59H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162198", "reactome_id": "R-ALL-2162198", "label": "CoQH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-163953", "reactome_id": "R-ALL-163953", "label": "H+", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-352609", "reactome_id": "R-HSA-352609", "label": "Cytochrome c (reduced)", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "quinol-cytochrome-c reductase activity of Cytochrome c reductase [mitochondrial inner membrane]", "activity_id": "164660", "ec_numbers": [ "7.1.1.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:186667", "title": "Possible molecular mechanisms of the protonmotive function of cytochrome", "journal": "J Theor Biol", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/186667/" }, { "id": "PMID:239860", "title": "Protonmotive redox mechanism of the cytochrome b-c1 complex in the", "journal": "FEBS Lett", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/239860/" } ], "x": 5008.666666666667, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-164832", "label": "ATPase synthesizes ATP", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "chemiosmosis" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:57722" ], "source_url": "https://reactome.org/content/detail/R-HSA-164832", "summary": "ATPase synthesizes ATP is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATPase:ADP:Pi to ATPase:ATP, H2O in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:57722.", "equation": "ATPase:ADP:Pi → ATPase:ATP + H2O", "inputs": [ { "node_id": "R-HSA-164838", "reactome_id": "R-HSA-164838", "label": "ATPase:ADP:Pi", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-164835", "reactome_id": "R-HSA-164835", "label": "ATPase:ATP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "proton-transporting ATP synthase activity, rotational mechanism of ATPase:ADP:Pi [mitochondrial inner membrane]", "activity_id": "164536", "ec_numbers": [ "7.1.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:4517936", "title": "A new concept for energy coupling in oxidative phosphorylation based on a", "journal": "Proc Natl Acad Sci U S A", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4517936/" } ], "x": 5670, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-164834", "label": "Enzyme-bound ATP is released", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "chemiosmosis" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:57722" ], "source_url": "https://reactome.org/content/detail/R-HSA-164834", "summary": "Enzyme-bound ATP is released is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATPase:ATP, H+ to F1Fo ATP synthase, ATP, H+ in mitochondrial matrix and cross-references RHEA:57722.", "equation": "ATPase:ATP + 3 H+ → F1Fo ATP synthase + ATP + 3 H+", "inputs": [ { "node_id": "R-HSA-164835", "reactome_id": "R-HSA-164835", "label": "ATPase:ATP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-163953", "reactome_id": "R-ALL-163953", "label": "H+", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-74186", "reactome_id": "R-HSA-74186", "label": "F1Fo ATP synthase", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "proton transmembrane transporter activity of ATPase:ATP [mitochondrial inner membrane]", "activity_id": "164850", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:4517936", "title": "A new concept for energy coupling in oxidative phosphorylation based on a", "journal": "Proc Natl Acad Sci U S A", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4517936/" } ], "x": 5391.333333333333, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-164840", "label": "ADP and Pi bind to ATPase", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "chemiosmosis" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:57722" ], "source_url": "https://reactome.org/content/detail/R-HSA-164840", "summary": "ADP and Pi bind to ATPase is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ADP, Pi, F1Fo ATP synthase to ATPase:ADP:Pi in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:57722.", "equation": "ADP + Pi + F1Fo ATP synthase → ATPase:ADP:Pi", "inputs": [ { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-74186", "reactome_id": "R-HSA-74186", "label": "F1Fo ATP synthase", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-164838", "reactome_id": "R-HSA-164838", "label": "ATPase:ADP:Pi", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:4517936", "title": "A new concept for energy coupling in oxidative phosphorylation based on a", "journal": "Proc Natl Acad Sci U S A", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4517936/" } ], "x": 5948.666666666667, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-165026", "label": "OCTN2 / SLC22A5 transports CAR from extracellular space to cytosol", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:72091" ], "source_url": "https://reactome.org/content/detail/R-HSA-165026", "summary": "OCTN2 / SLC22A5 transports CAR from extracellular space to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CAR, Na+ to CAR, Na+ in plasma membrane and cross-references RHEA:72091.", "equation": "CAR + Na+ → CAR + Na+", "inputs": [ { "node_id": "R-ALL-164988", "reactome_id": "R-ALL-164988", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-74113", "reactome_id": "R-ALL-74113", "label": "Na+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30235", "reactome_id": "R-ALL-30235", "label": "CAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-83910", "reactome_id": "R-ALL-83910", "label": "Na+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carnitine transmembrane transporter activity of SLC22A5 [plasma membrane]", "activity_id": "165001", "ec_numbers": null, "accessions": [ "O76082" ] } ], "citations": [ { "id": "PMID:9706223", "title": "Genetic disorders of carnitine metabolism and their nutritional management.", "journal": "Annu Rev Nutr", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9706223/" }, { "id": "PMID:26828774", "title": "Carnitine transport and fatty acid oxidation", "journal": "Biochim. Biophys. Acta", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26828774/" }, { "id": "PMID:10559218", "title": "Mutations in novel cation transporter (OCTN2), an organic cation/carnitine transporter,with differential effects on the organic cation transport function and the carnitine transport function", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10559218/" }, { "id": "PMID:9685390", "title": "Molecular and functional identification of sodium ion-dependent, high-affinity human carnitine transporter OCTN2", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9685390/" } ], "x": 590, "y": 1573.7368421052631, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1655443", "label": "PXKLP-K305-CSAD decarboxylates 3-sulfinoalanine to hypotaurine", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16878" ], "source_url": "https://reactome.org/content/detail/R-HSA-1655443", "summary": "PXKLP-K305-CSAD decarboxylates 3-sulfinoalanine to hypotaurine is a Reactome release 97 curated reaction event. The source record connects 3-Sulfinoalanine, H+ to HTAU, CO2 in cytosol and cross-references RHEA:16878.", "equation": "3-Sulfinoalanine + H+ → HTAU + CO2", "inputs": [ { "node_id": "R-ALL-1614519", "reactome_id": "R-ALL-1614519", "label": "3-Sulfinoalanine", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61085", "formula": "C3H6NO4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1655445", "reactome_id": "R-ALL-1655445", "label": "HTAU", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57853", "formula": "C2H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfinoalanine decarboxylase activity of PXKLP-K305-CSAD dimer [cytosol]", "activity_id": "1655436", "ec_numbers": [ "4.1.1.29" ], "accessions": [] } ], "citations": [ { "id": "PMID:236774", "title": "Rat liver cysteine sulfinate decarboxylase: purification, new appraisal of the molecular weight and determination of catalytic properties", "journal": "Biochim Biophys Acta", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/236774/" }, { "id": "PMID:26327310", "title": "Mammalian CSAD and GADL1 have distinct biochemical properties and patterns of brain expression", "journal": "Neurochem Int", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26327310/" } ], "x": 1808.6666666666667, "y": 2908.1111111111113, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1655453", "label": "FMO1:FAD oxidizes HTAU to TAU", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:69820" ], "source_url": "https://reactome.org/content/detail/R-HSA-1655453", "summary": "FMO1:FAD oxidizes HTAU to TAU is a Reactome release 97 curated reaction event. The source record connects HTAU, H+, NADPH to TAU, H2O, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:69820.", "equation": "HTAU + H+ + NADPH + O2 → TAU + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-1655445", "reactome_id": "R-ALL-1655445", "label": "HTAU", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57853", "formula": "C2H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159420", "reactome_id": "R-ALL-159420", "label": "TAU", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:507393", "formula": "C2H7NO3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "hypotaurine monooxygenase activity of FMO1:FAD [endoplasmic reticulum membrane]", "activity_id": "1655454", "ec_numbers": [ "1.14.13.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:32156684", "title": "Flavin-Containing Monooxygenase 1 Catalyzes the Production of Taurine from Hypotaurine", "journal": "Drug Metab Dispos", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32156684/" } ], "x": 1251.3333333333333, "y": 2812.5555555555557, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1655823", "label": "Expression of Isopentenyl-diphosphate Delta-isomerase 1 (IDI1)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655823", "summary": "Expression of Isopentenyl-diphosphate Delta-isomerase 1 (IDI1) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects IDI1 gene to IDI1 in nucleoplasm · cytosol.", "equation": "IDI1 gene → IDI1", "inputs": [ { "node_id": "R-HSA-2393982", "reactome_id": "R-HSA-2393982", "label": "IDI1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191358", "reactome_id": "R-HSA-191358", "label": "IDI1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8020941", "title": "A human promyelocyte mRNA transiently induced by TPA is homologous to yeast IPP isomerase", "journal": "Genomics", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8020941/" } ], "x": 5008.666666666667, "y": 1866.0075757575758, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655824", "label": "Expression of Farnesyl Diphosphate Synthase (FDPS)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655824", "summary": "Expression of Farnesyl Diphosphate Synthase (FDPS) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects FDPS gene to FDPS in nucleoplasm · cytosol.", "equation": "FDPS gene → FDPS", "inputs": [ { "node_id": "R-HSA-2393989", "reactome_id": "R-HSA-2393989", "label": "FDPS gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-981566", "reactome_id": "R-HSA-981566", "label": "FDPS", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:1968462", "title": "Isolation and sequence of the human farnesyl pyrophosphate synthetase cDNA. Coordinate regulation of the mRNAs for farnesyl pyrophosphate synthetase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and 3-hydroxy-3-methylglutaryl coenzyme A synthase by phorbol ester", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/1968462/" } ], "x": 4451.333333333333, "y": 1871.901515151515, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655825", "label": "SREBP1A,1C,2:SCAP binds CopII Coat Complex", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655825", "summary": "SREBP1A,1C,2:SCAP binds CopII Coat Complex is a Reactome release 97 curated reaction event. The source record connects SAR1B:GTP:SEC23:SEC24, SREBP1A,1C,2:SCAP to SREBP1A,1C,2:SCAP:Cop II Coat in endoplasmic reticulum membrane.", "equation": "4 SAR1B:GTP:SEC23:SEC24 + SREBP1A,1C,2:SCAP → SREBP1A,1C,2:SCAP:Cop II Coat", "inputs": [ { "node_id": "R-HSA-203997", "reactome_id": "R-HSA-203997", "label": "SAR1B:GTP:SEC23:SEC24", "role": "input", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2317520", "reactome_id": "R-HSA-2317520", "label": "SREBP1A,1C,2:SCAP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655765", "reactome_id": "R-HSA-1655765", "label": "SREBP1A,1C,2:SCAP:Cop II Coat", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo adaptor activity of SREBP1A,1C,2:SCAP [endoplasmic reticulum membrane]", "activity_id": "9987246", "ec_numbers": null, "accessions": [] } ], "citations": [], "x": 4451.333333333333, "y": 1818.8560606060605, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1655826", "label": "Expression of 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase (HMGCR)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655826", "summary": "Expression of 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase (HMGCR) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects HMGCR gene to HMGCR-1 in nucleoplasm · endoplasmic reticulum membrane.", "equation": "HMGCR gene → HMGCR-1", "inputs": [ { "node_id": "R-HSA-2393990", "reactome_id": "R-HSA-2393990", "label": "HMGCR gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191308", "reactome_id": "R-HSA-191308", "label": "HMGCR-1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8647822", "title": "A direct role for sterol regulatory element binding protein in activation of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8647822/" } ], "x": 4730, "y": 1871.901515151515, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655827", "label": "Expression of 7-Dehydrocholesterol Reductase (DHCR7)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655827", "summary": "Expression of 7-Dehydrocholesterol Reductase (DHCR7) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DHCR7 gene to DHCR7 in nucleoplasm · endoplasmic reticulum membrane.", "equation": "DHCR7 gene → DHCR7", "inputs": [ { "node_id": "R-HSA-2393971", "reactome_id": "R-HSA-2393971", "label": "DHCR7 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-196423", "reactome_id": "R-HSA-196423", "label": "DHCR7", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9634533", "title": "Mutations in the human sterol delta7-reductase gene at 11q12-13 cause Smith-Lemli-Opitz syndrome", "journal": "Am J Hum Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9634533/" } ], "x": 5008.666666666667, "y": 1871.901515151515, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655828", "label": "Expression of Lanosterol Synthase (LSS)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655828", "summary": "Expression of Lanosterol Synthase (LSS) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects LSS gene to LSS in nucleoplasm · endoplasmic reticulum membrane.", "equation": "LSS gene → LSS", "inputs": [ { "node_id": "R-HSA-2393967", "reactome_id": "R-HSA-2393967", "label": "LSS gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191371", "reactome_id": "R-HSA-191371", "label": "LSS", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:17925399", "title": "Histone deacetylase 3 down-regulates cholesterol synthesis through repression of lanosterol synthase gene expression", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17925399/" } ], "x": 4451.333333333333, "y": 1877.7954545454545, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655830", "label": "Expression of Acetyl CoA Carboxylase 2 (ACACB, ACC2)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655830", "summary": "Expression of Acetyl CoA Carboxylase 2 (ACACB, ACC2) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects ACACB gene to ACACB in nucleoplasm · cytosol.", "equation": "ACACB gene → ACACB", "inputs": [ { "node_id": "R-HSA-2393980", "reactome_id": "R-HSA-2393980", "label": "ACACB gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-52493", "reactome_id": "R-HSA-52493", "label": "ACACB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9099716", "title": "Human acetyl-CoA carboxylase 2. Molecular cloning, characterization, chromosomal mapping, and evidence for two isoforms.", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9099716/" } ], "x": 4730, "y": 1877.7954545454545, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655831", "label": "SREBP1A,1C,2 translocates to the nucleus", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655831", "summary": "SREBP1A,1C,2 translocates to the nucleus is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects SREBP1A,1C,2:Importin beta-1 to SREBP1A,1C,2:Importin beta-1 in cytosol · nucleoplasm.", "equation": "SREBP1A,1C,2:Importin beta-1 → SREBP1A,1C,2:Importin beta-1", "inputs": [ { "node_id": "R-HSA-2065556", "reactome_id": "R-HSA-2065556", "label": "SREBP1A,1C,2:Importin beta-1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2065543", "reactome_id": "R-HSA-2065543", "label": "SREBP1A,1C,2:Importin beta-1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8156598", "title": "SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis", "journal": "Cell", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8156598/" }, { "id": "PMID:8674110", "title": "Sterol-regulated release of SREBP-2 from cell membranes requires two sequential cleavages, one within a transmembrane segment", "journal": "Cell", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8674110/" }, { "id": "PMID:11283257", "title": "Dimerization of sterol regulatory element-binding protein 2 via the helix-loop-helix-leucine zipper domain is a prerequisite for its nuclear localization mediated by importin beta", "journal": "Mol Cell Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11283257/" }, { "id": "PMID:14645851", "title": "The structure of importin-beta bound to SREBP-2: nuclear import of a transcription factor", "journal": "Science", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14645851/" }, { "id": "PMID:10397761", "title": "Nuclear import of sterol regulatory element-binding protein-2, a basic helix-loop-helix-leucine zipper (bHLH-Zip)-containing transcription factor, occurs through the direct interaction of importin beta with HLH-Zip", "journal": "Mol Biol Cell", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10397761/" }, { "id": "PMID:9634703", "title": "Co-crystal structure of sterol regulatory element binding protein 1a at 2.3 A resolution", "journal": "Structure", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9634703/" } ], "x": 5008.666666666667, "y": 1877.7954545454545, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655832", "label": "Expression of Geranylgeranyl Pyrophosphate Synthase (GGPS1)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655832", "summary": "Expression of Geranylgeranyl Pyrophosphate Synthase (GGPS1) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects GGPS1 gene to GGPS1 in nucleoplasm · cytosol.", "equation": "GGPS1 gene → GGPS1", "inputs": [ { "node_id": "R-HSA-2393998", "reactome_id": "R-HSA-2393998", "label": "GGPS1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191415", "reactome_id": "R-HSA-191415", "label": "GGPS1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:18726356", "title": "cDNA cloning, chromosome mapping and expression characterization of human geranylgeranyl pyrophosphate synthase", "journal": "Sci China C Life Sci", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/18726356/" } ], "x": 4451.333333333333, "y": 1883.689393939394, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655833", "label": "Expression of TM7SF2", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655833", "summary": "Expression of TM7SF2 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects TM7SF2 gene to TM7SF2 in nucleoplasm · endoplasmic reticulum membrane.", "equation": "TM7SF2 gene → TM7SF2", "inputs": [ { "node_id": "R-HSA-2393984", "reactome_id": "R-HSA-2393984", "label": "TM7SF2 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-194710", "reactome_id": "R-HSA-194710", "label": "TM7SF2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20138239", "title": "Activation of TM7SF2 promoter by SREBP-2 depends on a new sterol regulatory element, a GC-box, and an inverted CCAAT-box", "journal": "Biochim Biophys Acta", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20138239/" } ], "x": 4730, "y": 1883.689393939394, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655834", "label": "SREBP1A,1C,2:SCAP translocates to the Golgi", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum membrane · ER to Golgi transport vesicle membrane · Golgi membrane", "compartment_ids": [ "GO:0005789", "GO:0012507", "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655834", "summary": "SREBP1A,1C,2:SCAP translocates to the Golgi is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects SREBP1A,1C,2:SCAP:Cop II Coat to SREBP1A,1C,2:SCAP in endoplasmic reticulum membrane · ER to Golgi transport vesicle membrane · Golgi membrane.", "equation": "SREBP1A,1C,2:SCAP:Cop II Coat → SREBP1A,1C,2:SCAP", "inputs": [ { "node_id": "R-HSA-1655765", "reactome_id": "R-HSA-1655765", "label": "SREBP1A,1C,2:SCAP:Cop II Coat", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655742", "reactome_id": "R-HSA-1655742", "label": "SREBP1A,1C,2:SCAP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [], "x": 5008.666666666667, "y": 1919.0530303030303, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655835", "label": "Expression of ELOVL6", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655835", "summary": "Expression of ELOVL6 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects ELOVL6 gene to ELOVL6 in nucleoplasm · endoplasmic reticulum membrane.", "equation": "ELOVL6 gene → ELOVL6", "inputs": [ { "node_id": "R-HSA-2393996", "reactome_id": "R-HSA-2393996", "label": "ELOVL6 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-548680", "reactome_id": "R-HSA-548680", "label": "ELOVL6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20937905", "title": "ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis", "journal": "Proc Natl Acad Sci U S A", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20937905/" } ], "x": 5008.666666666667, "y": 1883.689393939394, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655836", "label": "Expression of Diphosphomevalonate Decarboxylase (MVD)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655836", "summary": "Expression of Diphosphomevalonate Decarboxylase (MVD) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MVD gene to MVD in nucleoplasm · cytosol.", "equation": "MVD gene → MVD", "inputs": [ { "node_id": "R-HSA-2393981", "reactome_id": "R-HSA-2393981", "label": "MVD gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191317", "reactome_id": "R-HSA-191317", "label": "MVD", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8626466", "title": "Molecular cloning and expression of the cDNAs encoding human and yeast mevalonate pyrophosphate decarboxylase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8626466/" } ], "x": 4451.333333333333, "y": 1889.5833333333333, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655837", "label": "Expression of Glycerol-3-phosphate Acyltransferase (GPAM, GPAT)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · mitochondrial outer membrane", "compartment_ids": [ "GO:0005654", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655837", "summary": "Expression of Glycerol-3-phosphate Acyltransferase (GPAM, GPAT) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects GPAM gene to GPAM(1-828) in nucleoplasm · mitochondrial outer membrane.", "equation": "GPAM gene → GPAM(1-828)", "inputs": [ { "node_id": "R-HSA-2393968", "reactome_id": "R-HSA-2393968", "label": "GPAM gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-61855", "reactome_id": "R-HSA-61855", "label": "GPAM(1-828)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20719759", "title": "Expression and regulation of GPAT isoforms in cultured human keratinocytes and rodent epidermis", "journal": "J Lipid Res", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20719759/" } ], "x": 4730, "y": 1889.5833333333333, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655839", "label": "Expression of Phosphomevalonate Kinase (PMVK)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655839", "summary": "Expression of Phosphomevalonate Kinase (PMVK) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PMVK gene to PMVK in nucleoplasm · cytosol.", "equation": "PMVK gene → PMVK", "inputs": [ { "node_id": "R-HSA-2393994", "reactome_id": "R-HSA-2393994", "label": "PMVK gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191348", "reactome_id": "R-HSA-191348", "label": "PMVK", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8663599", "title": "Molecular cloning of human phosphomevalonate kinase and identification of a consensus peroxisomal targeting sequence", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8663599/" } ], "x": 5008.666666666667, "y": 1889.5833333333333, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655842", "label": "S1P hydrolyzes SREBP1A,1C,2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "Golgi membrane", "compartment_ids": [ "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655842", "summary": "S1P hydrolyzes SREBP1A,1C,2 is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C,2:SCAP to SCAP tetramer, SREBF1A,1C,2 cleaved by S1P in Golgi membrane.", "equation": "SREBP1A,1C,2:SCAP → SCAP tetramer + 4 SREBF1A,1C,2 cleaved by S1P", "inputs": [ { "node_id": "R-HSA-1655742", "reactome_id": "R-HSA-1655742", "label": "SREBP1A,1C,2:SCAP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655737", "reactome_id": "R-HSA-1655737", "label": "SCAP tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1655726", "reactome_id": "R-HSA-1655726", "label": "SREBF1A,1C,2 cleaved by S1P", "role": "output", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "serine-type endopeptidase activity of MBTPS1 [Golgi membrane]", "activity_id": "380995", "ec_numbers": [ "3.4.21.1" ], "accessions": [ "Q14703" ] } ], "citations": [ { "id": "PMID:10644685", "title": "Biosynthesis and enzymatic characterization of human SKI-1/S1P and the processing of its inhibitory prosegment", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10644685/" }, { "id": "PMID:17449569", "title": "Genistein downregulates SREBP-1 regulated gene expression by inhibiting site-1 protease expression in HepG2 cells", "journal": "J Nutr", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17449569/" }, { "id": "PMID:10428864", "title": "Autocatalytic processing of site-1 protease removes propeptide and permits cleavage of sterol regulatory element-binding proteins", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10428864/" }, { "id": "PMID:8674110", "title": "Sterol-regulated release of SREBP-2 from cell membranes requires two sequential cleavages, one within a transmembrane segment", "journal": "Cell", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8674110/" }, { "id": "PMID:8156598", "title": "SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis", "journal": "Cell", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8156598/" }, { "id": "PMID:9488713", "title": "Cleavage of sterol regulatory element-binding proteins (SREBPs) at site-1 requires interaction with SREBP cleavage-activating protein. Evidence from in vivo competition studies", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9488713/" } ], "x": 4451.333333333333, "y": 1759.9166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1655843", "label": "Expression of Fatty Acid Synthase (FASN)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655843", "summary": "Expression of Fatty Acid Synthase (FASN) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects FASN gene to FASN in nucleoplasm · cytosol.", "equation": "FASN gene → FASN", "inputs": [ { "node_id": "R-HSA-2393979", "reactome_id": "R-HSA-2393979", "label": "FASN gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-199233", "reactome_id": "R-HSA-199233", "label": "FASN", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:7567999", "title": "Human fatty acid synthase: properties and molecular cloning", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7567999/" } ], "x": 4451.333333333333, "y": 1895.4772727272727, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655844", "label": "Expression of Squalene Monooxygenase (SQLE)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655844", "summary": "Expression of Squalene Monooxygenase (SQLE) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects SQLE gene to SQLE in nucleoplasm · endoplasmic reticulum membrane.", "equation": "SQLE gene → SQLE", "inputs": [ { "node_id": "R-HSA-2393973", "reactome_id": "R-HSA-2393973", "label": "SQLE gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191395", "reactome_id": "R-HSA-191395", "label": "SQLE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8626488", "title": "Transcriptional regulation of squalene epoxidase by sterols and inhibitors in HeLa cells", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8626488/" } ], "x": 4730, "y": 1895.4772727272727, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655845", "label": "Expression of Acetyl CoA Carboxylase 1 (ACACA, ACC1)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655845", "summary": "Expression of Acetyl CoA Carboxylase 1 (ACACA, ACC1) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects ACACA gene to ACACA in nucleoplasm · cytosol.", "equation": "ACACA gene → ACACA", "inputs": [ { "node_id": "R-HSA-2393997", "reactome_id": "R-HSA-2393997", "label": "ACACA gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1660609", "reactome_id": "R-HSA-1660609", "label": "ACACA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:7732023", "title": "Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7732023/" } ], "x": 5008.666666666667, "y": 1895.4772727272727, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655846", "label": "Expression of Mevalonate Kinase (MVK)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655846", "summary": "Expression of Mevalonate Kinase (MVK) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MVK gene to MVK in nucleoplasm · cytosol.", "equation": "MVK gene → MVK", "inputs": [ { "node_id": "R-HSA-2393995", "reactome_id": "R-HSA-2393995", "label": "MVK gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191375", "reactome_id": "R-HSA-191375", "label": "MVK", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:1377680", "title": "Molecular cloning of human mevalonate kinase and identification of a missense mutation in the genetic disease mevalonic aciduria", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1377680/" } ], "x": 4451.333333333333, "y": 1901.371212121212, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655847", "label": "Expression of Lanosterol Demethylase (CYP51A1)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655847", "summary": "Expression of Lanosterol Demethylase (CYP51A1) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CYP51A1 gene to CYP51A1 in nucleoplasm · endoplasmic reticulum membrane.", "equation": "CYP51A1 gene → CYP51A1", "inputs": [ { "node_id": "R-HSA-5660427", "reactome_id": "R-HSA-5660427", "label": "CYP51A1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-194721", "reactome_id": "R-HSA-194721", "label": "CYP51A1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8619637", "title": "The ubiquitously expressed human CYP51 encodes lanosterol 14 alpha-demethylase, a cytochrome P450 whose expression is regulated by oxysterols", "journal": "Arch Biochem Biophys", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8619637/" } ], "x": 4730, "y": 1901.371212121212, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655848", "label": "Expression of Hydroxymethylglutaryl coenzyme A synthase (HMGCS1)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655848", "summary": "Expression of Hydroxymethylglutaryl coenzyme A synthase (HMGCS1) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects HMGCS1 gene to HMGCS1 in nucleoplasm · cytosol.", "equation": "HMGCS1 gene → HMGCS1", "inputs": [ { "node_id": "R-HSA-2393972", "reactome_id": "R-HSA-2393972", "label": "HMGCS1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191329", "reactome_id": "R-HSA-191329", "label": "HMGCS1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19088433", "title": "Transcriptional regulation of HMG-CoA synthase and HMG-CoA reductase genes by human ACBP", "journal": "Cell Physiol Biochem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/19088433/" }, { "id": "PMID:12177166", "title": "Transcriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterogenic genes", "journal": "J Lipid Res", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12177166/" } ], "x": 5008.666666666667, "y": 1901.371212121212, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655849", "label": "Expression of Acyl-CoA Desaturase (Stearoyl CoA Desaturase, SCD)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655849", "summary": "Expression of Acyl-CoA Desaturase (Stearoyl CoA Desaturase, SCD) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects SCD gene to SCD in nucleoplasm · endoplasmic reticulum membrane.", "equation": "SCD gene → SCD", "inputs": [ { "node_id": "R-HSA-6800051", "reactome_id": "R-HSA-6800051", "label": "SCD gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-400117", "reactome_id": "R-HSA-400117", "label": "SCD", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:7909540", "title": "Partial characterization of a cDNA for human stearoyl-CoA desaturase and changes in its mRNA expression in some normal and malignant tissues", "journal": "Int J Cancer", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7909540/" }, { "id": "PMID:10400691", "title": "Identification of conserved cis-elements and transcription factors required for sterol-regulated transcription of stearoyl-CoA desaturase 1 and 2", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10400691/" }, { "id": "PMID:11414710", "title": "Cloning and characterization of the human stearoyl-CoA desaturase gene promoter: transcriptional activation by sterol regulatory element binding protein and repression by polyunsaturated fatty acids and cholesterol", "journal": "Biochem Biophys Res Commun", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11414710/" } ], "x": 4451.333333333333, "y": 1907.2651515151515, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655850", "label": "Expression of Farnesyldiphosphate Farnesyltransferase (FDFT1, Squalene Synthase)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655850", "summary": "Expression of Farnesyldiphosphate Farnesyltransferase (FDFT1, Squalene Synthase) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects FDFT1 gene to FDFT1 in nucleoplasm · endoplasmic reticulum membrane.", "equation": "FDFT1 gene → FDFT1", "inputs": [ { "node_id": "R-HSA-2393991", "reactome_id": "R-HSA-2393991", "label": "FDFT1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-191369", "reactome_id": "R-HSA-191369", "label": "FDFT1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:7685352", "title": "Transcriptional regulation by lovastatin and 25-hydroxycholesterol in HepG2 cells and molecular cloning and expression of the cDNA for the human hepatic squalene synthase", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7685352/" } ], "x": 4730, "y": 1907.2651515151515, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655851", "label": "S2P hydrolyzes SREBP1A,1C,2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655851", "summary": "S2P hydrolyzes SREBP1A,1C,2 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects SREBF1A,1C,2 cleaved by S1P to SREBF1A,1C,2 cleaved by S2P in Golgi membrane · cytosol.", "equation": "SREBF1A,1C,2 cleaved by S1P → SREBF1A,1C,2 cleaved by S2P", "inputs": [ { "node_id": "R-HSA-1655726", "reactome_id": "R-HSA-1655726", "label": "SREBF1A,1C,2 cleaved by S1P", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655732", "reactome_id": "R-HSA-1655732", "label": "SREBF1A,1C,2 cleaved by S2P", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "metalloendopeptidase activity of MBTPS2 [Golgi membrane]", "activity_id": "381068", "ec_numbers": [ "3.4.24.11" ], "accessions": [ "O43462" ] } ], "citations": [ { "id": "PMID:10805775", "title": "Asparagine-proline sequence within membrane-spanning segment of SREBP triggers intramembrane cleavage by site-2 protease", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10805775/" }, { "id": "PMID:9659902", "title": "Complementation cloning of S2P, a gene encoding a putative metalloprotease required for intramembrane cleavage of SREBPs", "journal": "Mol Cell", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9659902/" }, { "id": "PMID:8156598", "title": "SREBP-1, a membrane-bound transcription factor released by sterol-regulated proteolysis", "journal": "Cell", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8156598/" }, { "id": "PMID:8674110", "title": "Sterol-regulated release of SREBP-2 from cell membranes requires two sequential cleavages, one within a transmembrane segment", "journal": "Cell", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8674110/" } ], "x": 4730, "y": 1759.9166666666667, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1655852", "label": "Expression of Lathosterol Oxidase (SC5D, SC5DL)", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005654", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655852", "summary": "Expression of Lathosterol Oxidase (SC5D, SC5DL) is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects SC5DL gene to SC5D in nucleoplasm · endoplasmic reticulum membrane.", "equation": "SC5DL gene → SC5D", "inputs": [ { "node_id": "R-HSA-2394001", "reactome_id": "R-HSA-2394001", "label": "SC5DL gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-195662", "reactome_id": "R-HSA-195662", "label": "SC5D", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8976377", "title": "Molecular cloning and mapping of a human cDNA (SC5DL) encoding a protein homologous to fungal sterol-C5-desaturase", "journal": "Cytogenet Cell Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8976377/" } ], "x": 5008.666666666667, "y": 1907.2651515151515, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1655879", "label": "Thiosulfate can transfer its sulfur atom to glutathione", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1655879", "summary": "Thiosulfate can transfer its sulfur atom to glutathione is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects S2O3(2-), H+, GSH to H+, sulfite(2-), S-sulfanylglutathionate in mitochondrial matrix.", "equation": "S2O3(2-) + H+ + 2 GSH → H+ + sulfite(2-) + S-sulfanylglutathionate", "inputs": [ { "node_id": "R-ALL-1614595", "reactome_id": "R-ALL-1614595", "label": "S2O3(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33542", "formula": "HO3S2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1655887", "reactome_id": "R-ALL-1655887", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16856", "formula": "C10H17N3O6S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1614538", "reactome_id": "R-ALL-1614538", "label": "sulfite(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17359", "formula": "O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9930111", "reactome_id": "R-ALL-9930111", "label": "S-sulfanylglutathionate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58905", "formula": "C10H16N3O6S2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "thiosulfate-thiol sulfurtransferase activity of TSTD1 [cytosol]", "activity_id": "9014530", "ec_numbers": [ "2.8.1.3" ], "accessions": [ "Q8NFU3" ] } ], "citations": [ { "id": "PMID:6361026", "title": "The catalytic mechanism of yeast thiosulfate reductase", "journal": "J Biol Chem", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6361026/" }, { "id": "PMID:24981631", "title": "Biosynthesis of a central intermediate in hydrogen sulfide metabolism by a novel human sulfurtransferase and its yeast ortholog", "journal": "Biochemistry", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24981631/" } ], "x": 1808.6666666666667, "y": 3022.777777777778, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-1667005", "label": "Heparanase (HPSE) cleaves heparan sulfate from its proteoglycan (lysosome)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1667005", "summary": "Heparanase (HPSE) cleaves heparan sulfate from its proteoglycan (lysosome) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, HS/HPIN-PGs to IdoA-GlcN6S-IdoA2S-GlcN3S- group, GlcA-Gal-Gal-Xyl-HS proteins in lysosomal lumen.", "equation": "H2O + HS/HPIN-PGs → IdoA-GlcN6S-IdoA2S-GlcN3S- group + GlcA-Gal-Gal-Xyl-HS proteins", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2090050", "reactome_id": "R-HSA-2090050", "label": "HS/HPIN-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2090033", "reactome_id": "R-ALL-2090033", "label": "IdoA-GlcN6S-IdoA2S-GlcN3S- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747403", "formula": "C47H68N2O56S5", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076551", "reactome_id": "R-HSA-2076551", "label": "GlcA-Gal-Gal-Xyl-HS proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "heparanase activity of HPSE dimer [lysosomal lumen]", "activity_id": "1666996", "ec_numbers": [ "3.2.1.166" ], "accessions": [] } ], "citations": [ { "id": "PMID:12441129", "title": "Human heparanase is localized within lysosomes in a stable form", "journal": "Exp Cell Res", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12441129/" }, { "id": "PMID:12927802", "title": "Heterodimer formation is essential for heparanase enzymatic activity", "journal": "Biochem Biophys Res Commun", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12927802/" }, { "id": "PMID:12213822", "title": "Structural recognition by recombinant human heparanase that plays critical roles in tumor metastasis. Hierarchical sulfate groups with different effects and the essential target disulfated trisaccharide sequence", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12213822/" }, { "id": "PMID:10446189", "title": "Human heparanase. Purification, characterization, cloning, and expression", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10446189/" } ], "x": 2938.666666666667, "y": 1019.0151515151515, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1675773", "label": "PI(3,4)P2 is phosphorylated to PI(3,4,5)P3 by PIP5K1A-C at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1675773", "summary": "PI(3,4)P2 is phosphorylated to PI(3,4,5)P3 by PIP5K1A-C at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(3,4)P2, ATP to PI(3,4,5)P3, ADP in plasma membrane · cytosol.", "equation": "PI(3,4)P2 + ATP → PI(3,4,5)P3 + ADP", "inputs": [ { "node_id": "R-ALL-202247", "reactome_id": "R-ALL-202247", "label": "PI(3,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-179838", "reactome_id": "R-ALL-179838", "label": "PI(3,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57836", "formula": "C11H13O22P4R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphatidylinositol kinase activity of PIP5K1A-C [cytosol]", "activity_id": "1806282", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9211928", "title": "Phosphatidylinositol-4-phosphate 5-kinase isozymes catalyze the synthesis of 3-phosphate-containing phosphatidylinositol signaling molecules", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9211928/" }, { "id": "PMID:9660759", "title": "Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660759/" }, { "id": "PMID:11604140", "title": "PIP kinase Igamma is the major PI(4,5)P(2) synthesizing enzyme at the synapse", "journal": "Neuron", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11604140/" }, { "id": "PMID:12422219", "title": "Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin", "journal": "Nature", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12422219/" }, { "id": "PMID:12847086", "title": "ARF6 stimulates clathrin/AP-2 recruitment to synaptic membranes by activating phosphatidylinositol phosphate kinase type Igamma", "journal": "J Cell Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12847086/" } ], "x": 2381.3333333333335, "y": 1796.7739726027398, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675776", "label": "PI5P is phosphorylated to PI(4,5)P2 by PIP4K2 dimers at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:12280" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675776", "summary": "PI5P is phosphorylated to PI(4,5)P2 by PIP4K2 dimers at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI5P, ATP to PI(4,5)P2, ADP in plasma membrane · cytosol and cross-references RHEA:12280.", "equation": "PI5P + ATP → PI(4,5)P2 + ADP", "inputs": [ { "node_id": "R-ALL-1806240", "reactome_id": "R-ALL-1806240", "label": "PI5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-179856", "reactome_id": "R-ALL-179856", "label": "PI(4,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol-5-phosphate 4-kinase activity of PIP4K2 dimers [cytosol]", "activity_id": "1806209", "ec_numbers": [ "2.7.1.149" ], "accessions": [] } ], "citations": [ { "id": "PMID:18753295", "title": "Localization of phosphatidylinositol phosphate kinase IIgamma in kidney to a membrane trafficking compartment within specialized cells of the nephron", "journal": "Am J Physiol Renal Physiol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18753295/" }, { "id": "PMID:9367159", "title": "A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate", "journal": "Nature", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9367159/" }, { "id": "PMID:19896968", "title": "Localization, regulation and function of type II phosphatidylinositol 5-phosphate 4-kinases", "journal": "Adv Enzyme Regul", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19896968/" }, { "id": "PMID:23758345", "title": "Evolutionarily conserved structural changes in phosphatidylinositol 5-phosphate 4-kinase (PI5P4K) isoforms are responsible for differences in enzyme activity and localization", "journal": "Biochem. J.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23758345/" }, { "id": "PMID:25495341", "title": "The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site", "journal": "Biochem. J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25495341/" } ], "x": 2938.666666666667, "y": 1572.9657534246576, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675780", "label": "PI is phosphorylated to PI4P by PI4K2A/B at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:19877" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675780", "summary": "PI is phosphorylated to PI4P by PI4K2A/B at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI, ATP to PI4P, ADP in plasma membrane · cytosol and cross-references RHEA:19877.", "equation": "PI + ATP → PI4P + ADP", "inputs": [ { "node_id": "R-ALL-426658", "reactome_id": "R-ALL-426658", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-392417", "reactome_id": "R-ALL-392417", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol 4-kinase activity of PI4K2A/2B [cytosol]", "activity_id": "1806244", "ec_numbers": [ "2.7.1.67" ], "accessions": [] } ], "citations": [ { "id": "PMID:11923287", "title": "Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11923287/" }, { "id": "PMID:11279162", "title": "Cloning of a human type II phosphatidylinositol 4-kinase reveals a novel lipid kinase family", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11279162/" }, { "id": "PMID:12324459", "title": "Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12324459/" } ], "x": 2381.3333333333335, "y": 1578.2945205479452, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675795", "label": "PI3P is dephosphorylated to PI by MTM proteins at the late endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane · cytosol", "compartment_ids": [ "GO:0031902", "GO:0005829" ], "rhea_ids": [ "RHEA:12316" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675795", "summary": "PI3P is dephosphorylated to PI by MTM proteins at the late endosome membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI3P to PI, Pi in late endosome membrane · cytosol and cross-references RHEA:12316.", "equation": "H2O + PI3P → PI + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806198", "reactome_id": "R-ALL-1806198", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806195", "reactome_id": "R-ALL-1806195", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate phosphatase activity of MTM1,MTMR2,MTMR4,MTMR7 [cytosol]", "activity_id": "1806246", "ec_numbers": [ "3.1.3.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:16787938", "title": "Systematic analysis of myotubularins: heteromeric interactions, subcellular localisation and endosome related functions", "journal": "J Cell Sci", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16787938/" }, { "id": "PMID:11733541", "title": "Myotubularin and MTMR2, phosphatidylinositol 3-phosphatases mutated in myotubular myopathy and type 4B Charcot-Marie-Tooth disease", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11733541/" }, { "id": "PMID:18524850", "title": "Sequential actions of myotubularin lipid phosphatases regulate endosomal PI(3)P and growth factor receptor trafficking", "journal": "Mol Biol Cell", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18524850/" }, { "id": "PMID:14660569", "title": "Production of phosphatidylinositol 5-phosphate by the phosphoinositide 3-phosphatase myotubularin in mammalian cells", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14660569/" }, { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" }, { "id": "PMID:14722070", "title": "Myotubularin regulates the function of the late endosome through the gram domain-phosphatidylinositol 3,5-bisphosphate interaction", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14722070/" } ], "x": 2660, "y": 1578.2945205479452, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675810", "label": "PI is phosphorylated to PI5P by PIP5K1A/B at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:44680" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675810", "summary": "PI is phosphorylated to PI5P by PIP5K1A/B at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI, ATP to PI5P, ADP in plasma membrane · cytosol and cross-references RHEA:44680.", "equation": "PI + ATP → PI5P + ADP", "inputs": [ { "node_id": "R-ALL-426658", "reactome_id": "R-ALL-426658", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1806240", "reactome_id": "R-ALL-1806240", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol-5-kinase activity of PIP5K1A/B [cytosol]", "activity_id": "1806279", "ec_numbers": [ "2.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9660759", "title": "Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660759/" } ], "x": 2938.666666666667, "y": 1578.2945205479452, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675813", "label": "PI is phosphorylated to PI4P by PI4KA/2B at the ER membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:19877" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675813", "summary": "PI is phosphorylated to PI4P by PI4KA/2B at the ER membrane is a Reactome release 97 curated reaction event. The source record connects PI, ATP to PI4P, ADP in endoplasmic reticulum membrane · cytosol and cross-references RHEA:19877.", "equation": "PI + ATP → PI4P + ADP", "inputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1806234", "reactome_id": "R-ALL-1806234", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol 4-kinase activity of PI4KA/2B [cytosol]", "activity_id": "1806239", "ec_numbers": [ "2.7.1.67" ], "accessions": [] } ], "citations": [ { "id": "PMID:10101268", "title": "Functional expression and characterisation of a new human phosphatidylinositol 4-kinase PI4K230", "journal": "Biochim Biophys Acta", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10101268/" }, { "id": "PMID:9148941", "title": "Subcellular locations of phosphatidylinositol 4-kinase isoforms", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9148941/" }, { "id": "PMID:12324459", "title": "Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12324459/" } ], "x": 2381.3333333333335, "y": 1583.623287671233, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675815", "label": "PI4P transports from the Golgi membrane to the plasma membrane", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · plasma membrane", "compartment_ids": [ "GO:0000139", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1675815", "summary": "PI4P transports from the Golgi membrane to the plasma membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PI4P to PI4P in Golgi membrane · plasma membrane.", "equation": "PI4P → PI4P", "inputs": [ { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-392417", "reactome_id": "R-ALL-392417", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20404150", "title": "Acute manipulation of Golgi phosphoinositides to assess their importance in cellular trafficking and signaling", "journal": "Proc Natl Acad Sci U S A", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20404150/" }, { "id": "PMID:15107860", "title": "FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P", "journal": "Nat Cell Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15107860/" }, { "id": "PMID:19508231", "title": "Immunocytochemical techniques reveal multiple, distinct cellular pools of PtdIns4P and PtdIns(4,5)P(2)", "journal": "Biochem J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19508231/" } ], "x": 2381.3333333333335, "y": 1908.678082191781, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1675824", "label": "PI(4,5)P2 is dephosphorylated to PI4P by OCRL/INPP5E at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:22764" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675824", "summary": "PI(4,5)P2 is dephosphorylated to PI4P by OCRL/INPP5E at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI(4,5)P2 to PI4P, Pi in Golgi membrane · cytosol and cross-references RHEA:22764.", "equation": "H2O + PI(4,5)P2 → PI4P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806165", "reactome_id": "R-ALL-1806165", "label": "PI(4,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity of OCRL/INPP5E [cytosol]", "activity_id": "1806225", "ec_numbers": [ "3.1.3.36" ], "accessions": [] } ], "citations": [ { "id": "PMID:10806194", "title": "Cloning and characterization of a 72-kDa inositol-polyphosphate 5-phosphatase localized to the Golgi network", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10806194/" }, { "id": "PMID:19668216", "title": "Mutations in INPP5E, encoding inositol polyphosphate-5-phosphatase E, link phosphatidyl inositol signaling to the ciliopathies", "journal": "Nat Genet", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19668216/" }, { "id": "PMID:10764818", "title": "The isolation and characterization of a cDNA encoding phospholipid-specific inositol polyphosphate 5-phosphatase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10764818/" }, { "id": "PMID:19211563", "title": "Differential clathrin binding and subcellular localization of OCRL1 splice isoforms", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19211563/" }, { "id": "PMID:8634694", "title": "Lowe syndrome, a deficiency of phosphatidylinositol 4,5-bisphosphate 5-phosphatase in the Golgi apparatus", "journal": "Hum Mol Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8634694/" }, { "id": "PMID:7761412", "title": "The protein deficient in Lowe syndrome is a phosphatidylinositol-4,5-bisphosphate 5-phosphatase", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7761412/" } ], "x": 2660, "y": 1583.623287671233, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675834", "label": "PI(3,4)P2 is transported from the plasma membrane to the early endosome membrane", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · early endosome membrane", "compartment_ids": [ "GO:0005886", "GO:0031901" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1675834", "summary": "PI(3,4)P2 is transported from the plasma membrane to the early endosome membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PI(3,4)P2 to PI(3,4)P2 in plasma membrane · early endosome membrane.", "equation": "PI(3,4)P2 → PI(3,4)P2", "inputs": [ { "node_id": "R-ALL-202247", "reactome_id": "R-ALL-202247", "label": "PI(3,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806238", "reactome_id": "R-ALL-1806238", "label": "PI(3,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:14604433", "title": "Detection of novel intracellular agonist responsive pools of phosphatidylinositol 3,4-bisphosphate using the TAPP1 pleckstrin homology domain in immunoelectron microscopy", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14604433/" }, { "id": "PMID:15716355", "title": "The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane", "journal": "Mol Biol Cell", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15716355/" } ], "x": 2660, "y": 1908.678082191781, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1675836", "label": "PI(3,5)P2 is dephosphorylated to PI3P by SYNJ at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:32955" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675836", "summary": "PI(3,5)P2 is dephosphorylated to PI3P by SYNJ at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(3,5)P2, H2O to PI3P, Pi in plasma membrane · cytosol and cross-references RHEA:32955.", "equation": "PI(3,5)P2 + H2O → PI3P + Pi", "inputs": [ { "node_id": "R-ALL-1806224", "reactome_id": "R-ALL-1806224", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1806276", "reactome_id": "R-ALL-1806276", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity of SYNJs,OCRL [cytosol]", "activity_id": "1806194", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:10224048", "title": "SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10224048/" }, { "id": "PMID:11084340", "title": "Synaptojanin 2, a novel Rac1 effector that regulates clathrin-mediated endocytosis", "journal": "Curr Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11084340/" }, { "id": "PMID:18093523", "title": "The dual phosphatase activity of synaptojanin1 is required for both efficient synaptic vesicle endocytosis and reavailability at nerve terminals", "journal": "Neuron", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/18093523/" } ], "x": 2938.666666666667, "y": 1583.623287671233, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675866", "label": "PI is phosphorylated to PI5P by PIKFYVE at the late endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane · cytosol", "compartment_ids": [ "GO:0031902", "GO:0005829" ], "rhea_ids": [ "RHEA:44680" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675866", "summary": "PI is phosphorylated to PI5P by PIKFYVE at the late endosome membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI to ADP, PI5P in late endosome membrane · cytosol and cross-references RHEA:44680.", "equation": "ATP + PI → ADP + PI5P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806195", "reactome_id": "R-ALL-1806195", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806212", "reactome_id": "R-ALL-1806212", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-5-kinase activity of PIKFYVE:VAC14:FIG4 [late endosome membrane]", "activity_id": "1806232", "ec_numbers": [ "2.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10419465", "title": "PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419465/" }, { "id": "PMID:12270933", "title": "Phosphatidylinositol 5-phosphate biosynthesis is linked to PIKfyve and is involved in osmotic response pathway in mammalian cells", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12270933/" } ], "x": 2381.3333333333335, "y": 1588.9520547945206, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675883", "label": "PI is phosphorylated to PI4P by PI4KB at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:19877" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675883", "summary": "PI is phosphorylated to PI4P by PI4KB at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI to ADP, PI4P in Golgi membrane · cytosol and cross-references RHEA:19877.", "equation": "ATP + PI → ADP + PI4P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1524146", "reactome_id": "R-ALL-1524146", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol 4-kinase activity of ARF1/3:GTP:PI4KB [Golgi membrane]", "activity_id": "1806156", "ec_numbers": [ "2.7.1.67" ], "accessions": [] } ], "citations": [ { "id": "PMID:9405935", "title": "Identification and characterization of a novel human phosphatidylinositol 4-kinase", "journal": "DNA Res", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9405935/" } ], "x": 2660, "y": 1588.9520547945206, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675896", "label": "PI(3,5)P2 transports from the early endosome membrane to the Golgi membrane", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · Golgi membrane", "compartment_ids": [ "GO:0031901", "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1675896", "summary": "PI(3,5)P2 transports from the early endosome membrane to the Golgi membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PI(3,5)P2 to PI(3,5)P2 in early endosome membrane · Golgi membrane.", "equation": "PI(3,5)P2 → PI(3,5)P2", "inputs": [ { "node_id": "R-ALL-1806205", "reactome_id": "R-ALL-1806205", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806290", "reactome_id": "R-ALL-1806290", "label": "PI(3,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16954148", "title": "The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport", "journal": "J Cell Sci", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16954148/" } ], "x": 2938.666666666667, "y": 1908.678082191781, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1675910", "label": "PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the late endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane · cytosol", "compartment_ids": [ "GO:0031902", "GO:0005829" ], "rhea_ids": [ "RHEA:13609" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675910", "summary": "PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the late endosome membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI3P to ADP, PI(3,5)P2 in late endosome membrane · cytosol and cross-references RHEA:13609.", "equation": "ATP + PI3P → ADP + PI(3,5)P2", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806198", "reactome_id": "R-ALL-1806198", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806289", "reactome_id": "R-ALL-1806289", "label": "PI(3,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-3-phosphate 5-kinase activity of PIKFYVE:VAC14:FIG4 [late endosome membrane]", "activity_id": "1806250", "ec_numbers": [ "2.7.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" }, { "id": "PMID:10419465", "title": "PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419465/" }, { "id": "PMID:17556371", "title": "Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17556371/" }, { "id": "PMID:11706043", "title": "Phosphatidylinositol 3-phosphate-interacting domains in PIKfyve. Binding specificity and role in PIKfyve. Endomenbrane localization", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11706043/" } ], "x": 2938.666666666667, "y": 1588.9520547945206, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675921", "label": "PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:13609" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675921", "summary": "PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI3P to ADP, PI(3,5)P2 in Golgi membrane · cytosol and cross-references RHEA:13609.", "equation": "ATP + PI3P → ADP + PI(3,5)P2", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806159", "reactome_id": "R-ALL-1806159", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806290", "reactome_id": "R-ALL-1806290", "label": "PI(3,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-3-phosphate 5-kinase activity of PIKFYVE:VAC14:FIG4 [Golgi membrane]", "activity_id": "1806283", "ec_numbers": [ "2.7.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:10419465", "title": "PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419465/" }, { "id": "PMID:10567352", "title": "Complementation analysis in PtdInsP kinase-deficient yeast mutants demonstrates that Schizosaccharomyces pombe and murine Fab1p homologues are phosphatidylinositol 3-phosphate 5-kinases", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10567352/" }, { "id": "PMID:17556371", "title": "Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17556371/" }, { "id": "PMID:18950639", "title": "ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality", "journal": "J Mol Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18950639/" }, { "id": "PMID:11706043", "title": "Phosphatidylinositol 3-phosphate-interacting domains in PIKfyve. Binding specificity and role in PIKfyve. Endomenbrane localization", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11706043/" } ], "x": 2381.3333333333335, "y": 1594.2808219178082, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675928", "label": "PI4P is phosphorylated to PI(3,4)P2 by PIK3C2A/G at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1675928", "summary": "PI4P is phosphorylated to PI(3,4)P2 by PIK3C2A/G at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI4P to ADP, PI(3,4)P2 in Golgi membrane · cytosol.", "equation": "ATP + PI4P → ADP + PI(3,4)P2", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806230", "reactome_id": "R-ALL-1806230", "label": "PI(3,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-4-phosphate 3-kinase activity of PIK3C2A/G [cytosol]", "activity_id": "1806285", "ec_numbers": [ "2.7.1.154" ], "accessions": [] } ], "citations": [ { "id": "PMID:10766823", "title": "The class II phosphoinositide 3-kinase PI3K-C2alpha is concentrated in the trans-Golgi network and present in clathrin-coated vesicles", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10766823/" }, { "id": "PMID:9516481", "title": "A novel class II phosphoinositide 3-kinase predominantly expressed in the liver and its enhanced expression during liver regeneration", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9516481/" }, { "id": "PMID:9878262", "title": "cDNA cloning of a third human C2-domain-containing class II phosphoinositide 3-kinase, PI3K-C2gamma, and chromosomal assignment of this gene (PIK3C2G) to 12p12", "journal": "Genomics", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9878262/" }, { "id": "PMID:10805725", "title": "Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors", "journal": "Mol Cell Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10805725/" } ], "x": 2660, "y": 1796.7739726027398, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675939", "label": "PI is phosphorylated to PI3P by PIK3C2A/3 at the early endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [ "RHEA:12709" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675939", "summary": "PI is phosphorylated to PI3P by PIK3C2A/3 at the early endosome membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI to ADP, PI3P in early endosome membrane · cytosol and cross-references RHEA:12709.", "equation": "ATP + PI → ADP + PI3P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806199", "reactome_id": "R-ALL-1806199", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806200", "reactome_id": "R-ALL-1806200", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-3-kinase activity of PIK3C2A/3 [cytosol]", "activity_id": "1806222", "ec_numbers": [ "2.7.1.137" ], "accessions": [] } ], "citations": [ { "id": "PMID:10766823", "title": "The class II phosphoinositide 3-kinase PI3K-C2alpha is concentrated in the trans-Golgi network and present in clathrin-coated vesicles", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10766823/" }, { "id": "PMID:10805725", "title": "Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors", "journal": "Mol Cell Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10805725/" }, { "id": "PMID:8999962", "title": "Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase complex", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/8999962/" }, { "id": "PMID:7628435", "title": "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system", "journal": "EMBO J", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7628435/" }, { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" } ], "x": 2660, "y": 1594.2808219178082, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675949", "label": "PI(3,4,5)P3 is dephosphorylated to PI(3,4)P2 by INPP5[2] at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1675949", "summary": "PI(3,4,5)P3 is dephosphorylated to PI(3,4)P2 by INPP5[2] at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(3,4,5)P3, H2O to PI(3,4)P2, Pi in plasma membrane · cytosol.", "equation": "PI(3,4,5)P3 + H2O → PI(3,4)P2 + Pi", "inputs": [ { "node_id": "R-ALL-179838", "reactome_id": "R-ALL-179838", "label": "PI(3,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57836", "formula": "C11H13O22P4R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-202247", "reactome_id": "R-ALL-202247", "label": "PI(3,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity of INPP5(2) [cytosol]", "activity_id": "1806226", "ec_numbers": [ "3.1.3.86" ], "accessions": [] } ], "citations": [ { "id": "PMID:10753883", "title": "Identification and characterization of a novel inositol polyphosphate 5-phosphatase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10753883/" }, { "id": "PMID:12536145", "title": "Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal growth factor stimulation", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12536145/" }, { "id": "PMID:8769125", "title": "Cloning and expression of a human placenta inositol 1,3,4,5-tetrakisphosphate and phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8769125/" }, { "id": "PMID:8723348", "title": "Multiple forms of an inositol polyphosphate 5-phosphatase form signaling complexes with Shc and Grb2", "journal": "Curr Biol", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8723348/" }, { "id": "PMID:10593988", "title": "Novel inositol polyphosphate 5-phosphatase localizes at membrane ruffles", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10593988/" }, { "id": "PMID:10822173", "title": "The phosphatidylinositol polyphosphate 5-phosphatase SHIP1 associates with the dok1 phosphoprotein in bcr-Abl transformed cells", "journal": "Cell Signal", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10822173/" } ], "x": 2938.666666666667, "y": 1796.7739726027398, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675961", "label": "PI is phosphorylated to PI3P by PIK3C2A/3 at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:12709" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675961", "summary": "PI is phosphorylated to PI3P by PIK3C2A/3 at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI to ADP, PI3P in Golgi membrane · cytosol and cross-references RHEA:12709.", "equation": "ATP + PI → ADP + PI3P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1524146", "reactome_id": "R-ALL-1524146", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806159", "reactome_id": "R-ALL-1806159", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-3-kinase activity of PIK3C2A/3 [cytosol]", "activity_id": "1806222", "ec_numbers": [ "2.7.1.137" ], "accessions": [] } ], "citations": [ { "id": "PMID:10766823", "title": "The class II phosphoinositide 3-kinase PI3K-C2alpha is concentrated in the trans-Golgi network and present in clathrin-coated vesicles", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10766823/" }, { "id": "PMID:8999962", "title": "Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase complex", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/8999962/" }, { "id": "PMID:7628435", "title": "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system", "journal": "EMBO J", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7628435/" }, { "id": "PMID:10805725", "title": "Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors", "journal": "Mol Cell Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10805725/" } ], "x": 2938.666666666667, "y": 1594.2808219178082, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675974", "label": "PI is phosphorylated to PI4P by PI4K2A/B at the early endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [ "RHEA:19877" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675974", "summary": "PI is phosphorylated to PI4P by PI4K2A/B at the early endosome membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI to ADP, PI4P in early endosome membrane · cytosol and cross-references RHEA:19877.", "equation": "ATP + PI → ADP + PI4P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806199", "reactome_id": "R-ALL-1806199", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806180", "reactome_id": "R-ALL-1806180", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol 4-kinase activity of PI4K2A/2B [cytosol]", "activity_id": "1806244", "ec_numbers": [ "2.7.1.67" ], "accessions": [] } ], "citations": [ { "id": "PMID:11923287", "title": "Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11923287/" } ], "x": 2381.3333333333335, "y": 1599.609589041096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675988", "label": "PI4P is dephosphorylated to PI by SYNJ at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:55652" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675988", "summary": "PI4P is dephosphorylated to PI by SYNJ at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI4P, H2O to PI, Pi in plasma membrane · cytosol and cross-references RHEA:55652.", "equation": "PI4P + H2O → PI + Pi", "inputs": [ { "node_id": "R-ALL-392417", "reactome_id": "R-ALL-392417", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426658", "reactome_id": "R-ALL-426658", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol phosphate 4-phosphatase activity of SYNJs,OCRL [cytosol]", "activity_id": "1806162", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15080793", "title": "InsP3-mediated intracellular calcium signalling is altered by expression of synaptojanin-1", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15080793/" }, { "id": "PMID:10224048", "title": "SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10224048/" }, { "id": "PMID:11084340", "title": "Synaptojanin 2, a novel Rac1 effector that regulates clathrin-mediated endocytosis", "journal": "Curr Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11084340/" }, { "id": "PMID:18093523", "title": "The dual phosphatase activity of synaptojanin1 is required for both efficient synaptic vesicle endocytosis and reavailability at nerve terminals", "journal": "Neuron", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/18093523/" } ], "x": 2660, "y": 1599.609589041096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1675994", "label": "PI3P is dephosphorylated to PI by SYNJ/MTMs at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:12316" ], "source_url": "https://reactome.org/content/detail/R-HSA-1675994", "summary": "PI3P is dephosphorylated to PI by SYNJ/MTMs at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI3P, H2O to PI, Pi in plasma membrane · cytosol and cross-references RHEA:12316.", "equation": "PI3P + H2O → PI + Pi", "inputs": [ { "node_id": "R-ALL-1806276", "reactome_id": "R-ALL-1806276", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426658", "reactome_id": "R-ALL-426658", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate phosphatase activity of MTM1,MTMR1,MTMR3,MTMR6,MTMR14,SYNJ1,SYNJ2 [cytosol]", "activity_id": "1806277", "ec_numbers": [ "3.1.3.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" }, { "id": "PMID:14660569", "title": "Production of phosphatidylinositol 5-phosphate by the phosphoinositide 3-phosphatase myotubularin in mammalian cells", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14660569/" }, { "id": "PMID:12646134", "title": "Phosphatidylinositol-5-phosphate activation and conserved substrate specificity of the myotubularin phosphatidylinositol 3-phosphatases", "journal": "Curr Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12646134/" }, { "id": "PMID:12118066", "title": "The PtdIns3P phosphatase myotubularin is a cytoplasmic protein that also localizes to Rac1-inducible plasma membrane ruffles", "journal": "J Cell Sci", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12118066/" }, { "id": "PMID:11733541", "title": "Myotubularin and MTMR2, phosphatidylinositol 3-phosphatases mutated in myotubular myopathy and type 4B Charcot-Marie-Tooth disease", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11733541/" }, { "id": "PMID:11302699", "title": "FYVE-DSP2, a FYVE domain-containing dual specificity protein phosphatase that dephosphorylates phosphotidylinositol 3-phosphate", "journal": "Exp Cell Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11302699/" } ], "x": 2938.666666666667, "y": 1599.609589041096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676005", "label": "PI(3,5)P2 is dephosphorylated to PI3P by FIG4 at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:32955" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676005", "summary": "PI(3,5)P2 is dephosphorylated to PI3P by FIG4 at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI(3,5)P2 to PI3P, Pi in Golgi membrane · cytosol and cross-references RHEA:32955.", "equation": "H2O + PI(3,5)P2 → PI3P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806290", "reactome_id": "R-ALL-1806290", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806159", "reactome_id": "R-ALL-1806159", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity of PIKFYVE:VAC14:FIG4 [Golgi membrane]", "activity_id": "1806273", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:17556371", "title": "Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17556371/" }, { "id": "PMID:18950639", "title": "ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality", "journal": "J Mol Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18950639/" } ], "x": 2381.3333333333335, "y": 1604.9383561643835, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676020", "label": "PI(3,5)P2 is dephosphorylated to PI3P by FIG4 at the late endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane · cytosol", "compartment_ids": [ "GO:0031902", "GO:0005829" ], "rhea_ids": [ "RHEA:32955" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676020", "summary": "PI(3,5)P2 is dephosphorylated to PI3P by FIG4 at the late endosome membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI(3,5)P2 to PI3P, Pi in late endosome membrane · cytosol and cross-references RHEA:32955.", "equation": "H2O + PI(3,5)P2 → PI3P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806289", "reactome_id": "R-ALL-1806289", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806198", "reactome_id": "R-ALL-1806198", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity of PIKFYVE:VAC14:FIG4 [late endosome membrane]", "activity_id": "1806249", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:17556371", "title": "Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17556371/" }, { "id": "PMID:18950639", "title": "ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality", "journal": "J Mol Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18950639/" } ], "x": 2660, "y": 1604.9383561643835, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676024", "label": "PI is phosphorylated to PI3P by PIK3C2A/3 at the late endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane · cytosol", "compartment_ids": [ "GO:0031902", "GO:0005829" ], "rhea_ids": [ "RHEA:12709" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676024", "summary": "PI is phosphorylated to PI3P by PIK3C2A/3 at the late endosome membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI to ADP, PI3P in late endosome membrane · cytosol and cross-references RHEA:12709.", "equation": "ATP + PI → ADP + PI3P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806195", "reactome_id": "R-ALL-1806195", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806198", "reactome_id": "R-ALL-1806198", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-3-kinase activity of PIK3C2A/3 [cytosol]", "activity_id": "1806222", "ec_numbers": [ "2.7.1.137" ], "accessions": [] } ], "citations": [ { "id": "PMID:10766823", "title": "The class II phosphoinositide 3-kinase PI3K-C2alpha is concentrated in the trans-Golgi network and present in clathrin-coated vesicles", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10766823/" }, { "id": "PMID:10805725", "title": "Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors", "journal": "Mol Cell Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10805725/" }, { "id": "PMID:8999962", "title": "Characterization of p150, an adaptor protein for the human phosphatidylinositol (PtdIns) 3-kinase. Substrate presentation by phosphatidylinositol transfer protein to the p150.Ptdins 3-kinase complex", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/8999962/" }, { "id": "PMID:7628435", "title": "A human phosphatidylinositol 3-kinase complex related to the yeast Vps34p-Vps15p protein sorting system", "journal": "EMBO J", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7628435/" }, { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" } ], "x": 2938.666666666667, "y": 1604.9383561643835, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676041", "label": "Presence of PI(3,5)P2 stimulates the maturation of the early endosome into the late endosome", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · late endosome membrane", "compartment_ids": [ "GO:0031901", "GO:0031902" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676041", "summary": "Presence of PI(3,5)P2 stimulates the maturation of the early endosome into the late endosome is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PI(3,5)P2 to PI(3,5)P2 in early endosome membrane · late endosome membrane.", "equation": "PI(3,5)P2 → PI(3,5)P2", "inputs": [ { "node_id": "R-ALL-1806205", "reactome_id": "R-ALL-1806205", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806289", "reactome_id": "R-ALL-1806289", "label": "PI(3,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16448788", "title": "Cloning and subcellular localization of a human phosphatidylinositol 3-phosphate 5-kinase, PIKfyve/Fab1", "journal": "Gene", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16448788/" }, { "id": "PMID:16510848", "title": "Localized PtdIns 3,5-P2 synthesis to regulate early endosome dynamics and fusion", "journal": "Am J Physiol Cell Physiol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16510848/" }, { "id": "PMID:11285266", "title": "Mammalian cell morphology and endocytic membrane homeostasis require enzymatically active phosphoinositide 5-kinase PIKfyve", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11285266/" } ], "x": 2381.3333333333335, "y": 1914.0068493150684, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1676048", "label": "PI(4,5)P2 is phosphorylated to PI(3,4,5)P3 by PIK3C[1] at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:21292" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676048", "summary": "PI(4,5)P2 is phosphorylated to PI(3,4,5)P3 by PIK3C[1] at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(4,5)P2, ATP to PI(3,4,5)P3, ADP in plasma membrane · cytosol and cross-references RHEA:21292.", "equation": "PI(4,5)P2 + ATP → PI(3,4,5)P3 + ADP", "inputs": [ { "node_id": "R-ALL-179856", "reactome_id": "R-ALL-179856", "label": "PI(4,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-179838", "reactome_id": "R-ALL-179838", "label": "PI(3,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57836", "formula": "C11H13O22P4R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol-4,5-bisphosphate 3-kinase activity of PIK3C(1) [cytosol]", "activity_id": "1806243", "ec_numbers": [ "2.7.1.153" ], "accessions": [] } ], "citations": [ { "id": "PMID:9094719", "title": "The G beta gamma sensitivity of a PI3K is dependent upon a tightly associated adaptor, p101", "journal": "Cell", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9094719/" }, { "id": "PMID:15797027", "title": "p84, a new Gbetagamma-activated regulatory subunit of the type IB phosphoinositide 3-kinase p110gamma", "journal": "Curr Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15797027/" }, { "id": "PMID:9524259", "title": "Cloning of human p55 gamma, a regulatory subunit of phosphatidylinositol 3-kinase, by a yeast two-hybrid library screen with the insulin-like growth factor-I receptor", "journal": "Gene", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9524259/" }, { "id": "PMID:16476736", "title": "Characterization of p87PIKAP, a novel regulatory subunit of phosphoinositide 3-kinase gamma that is highly expressed in heart and interacts with PDE3B", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16476736/" }, { "id": "PMID:9113989", "title": "P110delta, a novel phosphoinositide 3-kinase in leukocytes", "journal": "Proc Natl Acad Sci U S A", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9113989/" }, { "id": "PMID:15135396", "title": "Cloning, expression, purification, and characterization of the human Class Ia phosphoinositide 3-kinase isoforms", "journal": "Protein Expr Purif", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15135396/" } ], "x": 2381.3333333333335, "y": 1610.2671232876712, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676065", "label": "PI(3,5)P2 is dephosphorylated to PI5P by MTM proteins at the late endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane · cytosol", "compartment_ids": [ "GO:0031902", "GO:0005829" ], "rhea_ids": [ "RHEA:39019" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676065", "summary": "PI(3,5)P2 is dephosphorylated to PI5P by MTM proteins at the late endosome membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI(3,5)P2 to PI5P, Pi in late endosome membrane · cytosol and cross-references RHEA:39019.", "equation": "H2O + PI(3,5)P2 → PI5P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806289", "reactome_id": "R-ALL-1806289", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806212", "reactome_id": "R-ALL-1806212", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity of MTM1,MTMR2,MTMR4,MTMR7 [cytosol]", "activity_id": "1806254", "ec_numbers": [ "3.1.3.95" ], "accessions": [] } ], "citations": [ { "id": "PMID:16787938", "title": "Systematic analysis of myotubularins: heteromeric interactions, subcellular localisation and endosome related functions", "journal": "J Cell Sci", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16787938/" }, { "id": "PMID:14722070", "title": "Myotubularin regulates the function of the late endosome through the gram domain-phosphatidylinositol 3,5-bisphosphate interaction", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14722070/" }, { "id": "PMID:14660569", "title": "Production of phosphatidylinositol 5-phosphate by the phosphoinositide 3-phosphatase myotubularin in mammalian cells", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14660569/" }, { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" }, { "id": "PMID:18524850", "title": "Sequential actions of myotubularin lipid phosphatases regulate endosomal PI(3)P and growth factor receptor trafficking", "journal": "Mol Biol Cell", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18524850/" }, { "id": "PMID:12890864", "title": "Characterization of myotubularin-related protein 7 and its binding partner, myotubularin-related protein 9", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12890864/" } ], "x": 2660, "y": 1610.2671232876712, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676082", "label": "PI4P is phosphorylated to PI(4,5)P2 by PIP5K1A-C at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:14425" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676082", "summary": "PI4P is phosphorylated to PI(4,5)P2 by PIP5K1A-C at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI4P, ATP to PI(4,5)P2, ADP in plasma membrane · cytosol and cross-references RHEA:14425.", "equation": "PI4P + ATP → PI(4,5)P2 + ADP", "inputs": [ { "node_id": "R-ALL-392417", "reactome_id": "R-ALL-392417", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-179856", "reactome_id": "R-ALL-179856", "label": "PI(4,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol-4-phosphate 5-kinase activity of PIP5K1A-C [cytosol]", "activity_id": "1806262", "ec_numbers": [ "2.7.1.68" ], "accessions": [] } ], "citations": [ { "id": "PMID:9211928", "title": "Phosphatidylinositol-4-phosphate 5-kinase isozymes catalyze the synthesis of 3-phosphate-containing phosphatidylinositol signaling molecules", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9211928/" }, { "id": "PMID:12422219", "title": "Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin", "journal": "Nature", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12422219/" }, { "id": "PMID:16979564", "title": "A role for PtdIns(4,5)P2 and PIP5Kalpha in regulating stress-induced apoptosis", "journal": "Curr Biol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16979564/" }, { "id": "PMID:15464023", "title": "Regulation and cellular roles of phosphoinositide 5-kinases", "journal": "Eur J Pharmacol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15464023/" }, { "id": "PMID:17245604", "title": "Phospholipase D signaling: orchestration by PIP2 and small GTPases", "journal": "Naunyn Schmiedebergs Arch Pharmacol", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17245604/" } ], "x": 2938.666666666667, "y": 1610.2671232876712, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676105", "label": "PI(3,5)P2 is dephosphorylated to PI5P by MTM proteins at the early endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [ "RHEA:39019" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676105", "summary": "PI(3,5)P2 is dephosphorylated to PI5P by MTM proteins at the early endosome membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI(3,5)P2 to PI5P, Pi in early endosome membrane · cytosol and cross-references RHEA:39019.", "equation": "H2O + PI(3,5)P2 → PI5P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806205", "reactome_id": "R-ALL-1806205", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806259", "reactome_id": "R-ALL-1806259", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity of MTM1,MTMR2,MTMR4 [cytosol]", "activity_id": "1806176", "ec_numbers": [ "3.1.3.95" ], "accessions": [] } ], "citations": [ { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" }, { "id": "PMID:18524850", "title": "Sequential actions of myotubularin lipid phosphatases regulate endosomal PI(3)P and growth factor receptor trafficking", "journal": "Mol Biol Cell", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18524850/" }, { "id": "PMID:16787938", "title": "Systematic analysis of myotubularins: heteromeric interactions, subcellular localisation and endosome related functions", "journal": "J Cell Sci", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16787938/" } ], "x": 2381.3333333333335, "y": 1615.5958904109589, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676109", "label": "PI4P is phosphorylated to PI(3,4)P2 by PI3K3C[2] at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676109", "summary": "PI4P is phosphorylated to PI(3,4)P2 by PI3K3C[2] at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI4P, ATP to PI(3,4)P2, ADP in plasma membrane · cytosol.", "equation": "PI4P + ATP → PI(3,4)P2 + ADP", "inputs": [ { "node_id": "R-ALL-392417", "reactome_id": "R-ALL-392417", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-202247", "reactome_id": "R-ALL-202247", "label": "PI(3,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol-4-phosphate 3-kinase activity of PIK3(2) [cytosol]", "activity_id": "1806197", "ec_numbers": [ "2.7.1.154" ], "accessions": [] } ], "citations": [ { "id": "PMID:7624799", "title": "Cloning and characterization of a G protein-activated human phosphoinositide-3 kinase", "journal": "Science", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7624799/" }, { "id": "PMID:15797027", "title": "p84, a new Gbetagamma-activated regulatory subunit of the type IB phosphoinositide 3-kinase p110gamma", "journal": "Curr Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15797027/" }, { "id": "PMID:9286278", "title": "Phosphatidylinositol 3-kinase in bovine photoreceptor rod outer segments", "journal": "Invest Ophthalmol Vis Sci", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9286278/" }, { "id": "PMID:15135396", "title": "Cloning, expression, purification, and characterization of the human Class Ia phosphoinositide 3-kinase isoforms", "journal": "Protein Expr Purif", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15135396/" }, { "id": "PMID:9514948", "title": "Cloning and characterization of a novel class II phosphoinositide 3-kinase containing C2 domain", "journal": "Biochem Biophys Res Commun", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9514948/" }, { "id": "PMID:9516481", "title": "A novel class II phosphoinositide 3-kinase predominantly expressed in the liver and its enhanced expression during liver regeneration", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9516481/" } ], "x": 2381.3333333333335, "y": 1802.1027397260275, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676114", "label": "PI3P is dephosphorylated to PI by SACM1L at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:12316" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676114", "summary": "PI3P is dephosphorylated to PI by SACM1L at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI3P to PI, Pi in Golgi membrane · cytosol and cross-references RHEA:12316.", "equation": "H2O + PI3P → PI + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806159", "reactome_id": "R-ALL-1806159", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524146", "reactome_id": "R-ALL-1524146", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate phosphatase activity of SACM1L [Golgi membrane]", "activity_id": "1806284", "ec_numbers": [ "3.1.3.64" ], "accessions": [ "Q9NTJ5" ] } ], "citations": [ { "id": "PMID:14527956", "title": "The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14527956/" } ], "x": 2660, "y": 1615.5958904109589, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676124", "label": "PI4P is dephosphorylated to PI by SACM1L at the ER membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:55652" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676124", "summary": "PI4P is dephosphorylated to PI by SACM1L at the ER membrane is a Reactome release 97 curated reaction event. The source record connects PI4P, H2O to PI, Pi in endoplasmic reticulum membrane · cytosol and cross-references RHEA:55652.", "equation": "PI4P + H2O → PI + Pi", "inputs": [ { "node_id": "R-ALL-1806234", "reactome_id": "R-ALL-1806234", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol phosphate 4-phosphatase activity of SACM1L [endoplasmic reticulum membrane]", "activity_id": "1806179", "ec_numbers": null, "accessions": [ "Q9NTJ5" ] } ], "citations": [ { "id": "PMID:14527956", "title": "The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14527956/" } ], "x": 2938.666666666667, "y": 1615.5958904109589, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676133", "label": "PI4P is dephosphorylated to PI by SACM1L at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:55652" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676133", "summary": "PI4P is dephosphorylated to PI by SACM1L at the Golgi membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PI4P to PI, Pi in Golgi membrane · cytosol and cross-references RHEA:55652.", "equation": "PI4P → PI + Pi", "inputs": [ { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1524146", "reactome_id": "R-ALL-1524146", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol phosphate 4-phosphatase activity of SACM1L [Golgi membrane]", "activity_id": "1806172", "ec_numbers": null, "accessions": [ "Q9NTJ5" ] } ], "citations": [ { "id": "PMID:14527956", "title": "The human phosphatidylinositol phosphatase SAC1 interacts with the coatomer I complex", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14527956/" } ], "x": 2938.666666666667, "y": 1658.2260273972602, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1676134", "label": "PI3P is phosphorylated to PI(3,5)P2 by PIP5K1A/B at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:13609" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676134", "summary": "PI3P is phosphorylated to PI(3,5)P2 by PIP5K1A/B at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI3P, ATP to PI(3,5)P2, ADP in plasma membrane · cytosol and cross-references RHEA:13609.", "equation": "PI3P + ATP → PI(3,5)P2 + ADP", "inputs": [ { "node_id": "R-ALL-1806276", "reactome_id": "R-ALL-1806276", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1806224", "reactome_id": "R-ALL-1806224", "label": "PI(3,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol-3-phosphate 5-kinase activity of PIP5K1A/B [cytosol]", "activity_id": "1806190", "ec_numbers": [ "2.7.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:9660759", "title": "Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660759/" } ], "x": 2381.3333333333335, "y": 1620.9246575342465, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676141", "label": "PI3P is dephosphorylated to PI by MTM proteins at the early endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [ "RHEA:12316" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676141", "summary": "PI3P is dephosphorylated to PI by MTM proteins at the early endosome membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI3P to PI, Pi in early endosome membrane · cytosol and cross-references RHEA:12316.", "equation": "H2O + PI3P → PI + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806200", "reactome_id": "R-ALL-1806200", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806199", "reactome_id": "R-ALL-1806199", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate phosphatase activity of MTM1,MTMR2,MTMR4 [cytosol]", "activity_id": "1806170", "ec_numbers": [ "3.1.3.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:11302699", "title": "FYVE-DSP2, a FYVE domain-containing dual specificity protein phosphatase that dephosphorylates phosphotidylinositol 3-phosphate", "journal": "Exp Cell Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11302699/" }, { "id": "PMID:16787938", "title": "Systematic analysis of myotubularins: heteromeric interactions, subcellular localisation and endosome related functions", "journal": "J Cell Sci", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16787938/" }, { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" }, { "id": "PMID:18524850", "title": "Sequential actions of myotubularin lipid phosphatases regulate endosomal PI(3)P and growth factor receptor trafficking", "journal": "Mol Biol Cell", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18524850/" }, { "id": "PMID:11733541", "title": "Myotubularin and MTMR2, phosphatidylinositol 3-phosphatases mutated in myotubular myopathy and type 4B Charcot-Marie-Tooth disease", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11733541/" } ], "x": 2660, "y": 1620.9246575342465, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676145", "label": "PI3P is phosphorylated to PI(3,4)P2 by PIP4K2/5K1 at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676145", "summary": "PI3P is phosphorylated to PI(3,4)P2 by PIP4K2/5K1 at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI3P, ATP to PI(3,4)P2, ADP in plasma membrane · cytosol.", "equation": "PI3P + ATP → PI(3,4)P2 + ADP", "inputs": [ { "node_id": "R-ALL-1806276", "reactome_id": "R-ALL-1806276", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-202247", "reactome_id": "R-ALL-202247", "label": "PI(3,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-phosphatidylinositol-3-phosphate 4-kinase activity of PIP4K2/5K1 [cytosol]", "activity_id": "1806274", "ec_numbers": [ "2.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:19896968", "title": "Localization, regulation and function of type II phosphatidylinositol 5-phosphate 4-kinases", "journal": "Adv Enzyme Regul", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19896968/" }, { "id": "PMID:9211928", "title": "Phosphatidylinositol-4-phosphate 5-kinase isozymes catalyze the synthesis of 3-phosphate-containing phosphatidylinositol signaling molecules", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9211928/" }, { "id": "PMID:9367159", "title": "A new pathway for synthesis of phosphatidylinositol-4,5-bisphosphate", "journal": "Nature", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9367159/" }, { "id": "PMID:9660759", "title": "Type I phosphatidylinositol-4-phosphate 5-kinases synthesize the novel lipids phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 5-phosphate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660759/" }, { "id": "PMID:18753295", "title": "Localization of phosphatidylinositol phosphate kinase IIgamma in kidney to a membrane trafficking compartment within specialized cells of the nephron", "journal": "Am J Physiol Renal Physiol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18753295/" }, { "id": "PMID:12422219", "title": "Recruitment and regulation of phosphatidylinositol phosphate kinase type 1 gamma by the FERM domain of talin", "journal": "Nature", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12422219/" } ], "x": 2660, "y": 1802.1027397260275, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676149", "label": "PI(3,4)P2 is dephosphorylated to PI4P by PTEN at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676149", "summary": "PI(3,4)P2 is dephosphorylated to PI4P by PTEN at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(3,4)P2, H2O to PI4P, Pi in plasma membrane · cytosol.", "equation": "PI(3,4)P2 + H2O → PI4P + Pi", "inputs": [ { "node_id": "R-ALL-202247", "reactome_id": "R-ALL-202247", "label": "PI(3,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-392417", "reactome_id": "R-ALL-392417", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity of PTEN [cytosol]", "activity_id": "1806248", "ec_numbers": null, "accessions": [ "P60484" ] } ], "citations": [ { "id": "PMID:9811831", "title": "The lipid phosphatase activity of PTEN is critical for its tumor supressor function", "journal": "Proc Natl Acad Sci U S A", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9811831/" }, { "id": "PMID:12808147", "title": "Membrane-binding and activation mechanism of PTEN", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12808147/" }, { "id": "PMID:10555148", "title": "Crystal structure of the PTEN tumor suppressor: implications for its phosphoinositide phosphatase activity and membrane association", "journal": "Cell", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10555148/" }, { "id": "PMID:8681945", "title": "Purification and characterization of the phosphatidylinositol-3,4,5-trisphosphate phosphatase in bovine thymus", "journal": "Eur. J. Biochem.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8681945/" } ], "x": 2938.666666666667, "y": 1802.1027397260275, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676152", "label": "PI4KB binds to ARF1/3:GTP at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676152", "summary": "PI4KB binds to ARF1/3:GTP at the Golgi membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ARF1/3:GTP, PI4KB to ARF1/3:GTP:PI4KB in Golgi membrane · cytosol.", "equation": "ARF1/3:GTP + PI4KB → ARF1/3:GTP:PI4KB", "inputs": [ { "node_id": "R-HSA-1806258", "reactome_id": "R-HSA-1806258", "label": "ARF1/3:GTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-1604615", "reactome_id": "R-HSA-1604615", "label": "PI4KB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1806287", "reactome_id": "R-HSA-1806287", "label": "ARF1/3:GTP:PI4KB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol 4-kinase activator activity of ARF1/3:GTP [Golgi membrane]", "activity_id": "9947642", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:17555535", "title": "Specificity, promiscuity and localization of ARF protein interactions with NCS-1 and phosphatidylinositol-4 kinase-III beta", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17555535/" }, { "id": "PMID:9148941", "title": "Subcellular locations of phosphatidylinositol 4-kinase isoforms", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9148941/" }, { "id": "PMID:10559940", "title": "ARF mediates recruitment of PtdIns-4-OH kinase-beta and stimulates synthesis of PtdIns(4,5)P2 on the Golgi complex", "journal": "Nat Cell Biol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10559940/" } ], "x": 2938.666666666667, "y": 1876.7054794520548, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1676161", "label": "PI(3,5)P2 transports from the late endosome membrane to the Golgi membrane", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane · Golgi membrane", "compartment_ids": [ "GO:0031902", "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676161", "summary": "PI(3,5)P2 transports from the late endosome membrane to the Golgi membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PI(3,5)P2 to PI(3,5)P2 in late endosome membrane · Golgi membrane.", "equation": "PI(3,5)P2 → PI(3,5)P2", "inputs": [ { "node_id": "R-ALL-1806289", "reactome_id": "R-ALL-1806289", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "late endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806290", "reactome_id": "R-ALL-1806290", "label": "PI(3,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16954148", "title": "The mammalian phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) regulates endosome-to-TGN retrograde transport", "journal": "J Cell Sci", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16954148/" } ], "x": 2660, "y": 1914.0068493150684, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1676162", "label": "PI(3,4)P2 is dephosphorylated to PI3P by INPP4A/B at the early endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676162", "summary": "PI(3,4)P2 is dephosphorylated to PI3P by INPP4A/B at the early endosome membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI(3,4)P2 to PI3P, Pi in early endosome membrane · cytosol.", "equation": "H2O + PI(3,4)P2 → PI3P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806238", "reactome_id": "R-ALL-1806238", "label": "PI(3,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806200", "reactome_id": "R-ALL-1806200", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity of INPP4A/B [cytosol]", "activity_id": "1806174", "ec_numbers": [ "3.1.3.66" ], "accessions": [] } ], "citations": [ { "id": "PMID:7608176", "title": "The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphatase", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7608176/" }, { "id": "PMID:9295334", "title": "The cDNA cloning and characterization of inositol polyphosphate 4-phosphatase type II. Evidence for conserved alternative splicing in the 4-phosphatase family", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9295334/" }, { "id": "PMID:15716355", "title": "The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane", "journal": "Mol Biol Cell", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15716355/" } ], "x": 2381.3333333333335, "y": 1807.4315068493152, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676164", "label": "PI(3,4)P2 is dephosphorylated to PI3P by INPP4A/B at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676164", "summary": "PI(3,4)P2 is dephosphorylated to PI3P by INPP4A/B at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(3,4)P2, H2O to PI3P, Pi in plasma membrane · cytosol.", "equation": "PI(3,4)P2 + H2O → PI3P + Pi", "inputs": [ { "node_id": "R-ALL-202247", "reactome_id": "R-ALL-202247", "label": "PI(3,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1806276", "reactome_id": "R-ALL-1806276", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,4-bisphosphate 4-phosphatase activity of INPP4A/B [cytosol]", "activity_id": "1806174", "ec_numbers": [ "3.1.3.66" ], "accessions": [] } ], "citations": [ { "id": "PMID:7608176", "title": "The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphatase", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7608176/" }, { "id": "PMID:9295334", "title": "The cDNA cloning and characterization of inositol polyphosphate 4-phosphatase type II. Evidence for conserved alternative splicing in the 4-phosphatase family", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9295334/" }, { "id": "PMID:15716355", "title": "The type Ialpha inositol polyphosphate 4-phosphatase generates and terminates phosphoinositide 3-kinase signals on endosomes and the plasma membrane", "journal": "Mol Biol Cell", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15716355/" } ], "x": 2660, "y": 1807.4315068493152, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676168", "label": "PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the early endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [ "RHEA:13609" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676168", "summary": "PI3P is phosphorylated to PI(3,5)P2 by PIKFYVE at the early endosome membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI3P to ADP, PI(3,5)P2 in early endosome membrane · cytosol and cross-references RHEA:13609.", "equation": "ATP + PI3P → ADP + PI(3,5)P2", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806200", "reactome_id": "R-ALL-1806200", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806205", "reactome_id": "R-ALL-1806205", "label": "PI(3,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-3-phosphate 5-kinase activity of PIKFYVE:VAC14:FIG4 [early endosome membrane]", "activity_id": "1806278", "ec_numbers": [ "2.7.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" }, { "id": "PMID:10419465", "title": "PIKfyve, a mammalian ortholog of yeast Fab1p lipid kinase, synthesizes 5-phosphoinositides. Effect of insulin", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419465/" }, { "id": "PMID:17556371", "title": "Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17556371/" }, { "id": "PMID:11706043", "title": "Phosphatidylinositol 3-phosphate-interacting domains in PIKfyve. Binding specificity and role in PIKfyve. Endomenbrane localization", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11706043/" } ], "x": 2938.666666666667, "y": 1620.9246575342465, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676174", "label": "PI(3,5)P2 is dephosphorylated to PI3P by FIG4 at the early endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [ "RHEA:32955" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676174", "summary": "PI(3,5)P2 is dephosphorylated to PI3P by FIG4 at the early endosome membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI(3,5)P2 to PI3P, Pi in early endosome membrane · cytosol and cross-references RHEA:32955.", "equation": "H2O + PI(3,5)P2 → PI3P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806205", "reactome_id": "R-ALL-1806205", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806200", "reactome_id": "R-ALL-1806200", "label": "PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 5-phosphatase activity of PIKFYVE:VAC14:FIG4 [early endosome membrane]", "activity_id": "1806268", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:17556371", "title": "Core protein machinery for mammalian phosphatidylinositol 3,5-bisphosphate synthesis and turnover that regulates the progression of endosomal transport. Novel Sac phosphatase joins the ArPIKfyve-PIKfyve complex", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17556371/" }, { "id": "PMID:18950639", "title": "ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality", "journal": "J Mol Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18950639/" } ], "x": 2381.3333333333335, "y": 1626.2534246575342, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676177", "label": "PI(4,5)P2 is dephosphorylated to PI4P by SYNJ/INPP5[1] at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:22764" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676177", "summary": "PI(4,5)P2 is dephosphorylated to PI4P by SYNJ/INPP5[1] at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(4,5)P2, H2O to PI4P, Pi in plasma membrane · cytosol and cross-references RHEA:22764.", "equation": "PI(4,5)P2 + H2O → PI4P + Pi", "inputs": [ { "node_id": "R-ALL-179856", "reactome_id": "R-ALL-179856", "label": "PI(4,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-392417", "reactome_id": "R-ALL-392417", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity of SYNJ/INPP5(1) [cytosol]", "activity_id": "1806203", "ec_numbers": [ "3.1.3.36" ], "accessions": [] } ], "citations": [ { "id": "PMID:10753883", "title": "Identification and characterization of a novel inositol polyphosphate 5-phosphatase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10753883/" }, { "id": "PMID:12536145", "title": "Identification of a novel domain in two mammalian inositol-polyphosphate 5-phosphatases that mediates membrane ruffle localization. The inositol 5-phosphatase skip localizes to the endoplasmic reticulum and translocates to membrane ruffles following epidermal growth factor stimulation", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12536145/" }, { "id": "PMID:10593988", "title": "Novel inositol polyphosphate 5-phosphatase localizes at membrane ruffles", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10593988/" }, { "id": "PMID:18093523", "title": "The dual phosphatase activity of synaptojanin1 is required for both efficient synaptic vesicle endocytosis and reavailability at nerve terminals", "journal": "Neuron", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/18093523/" }, { "id": "PMID:10224048", "title": "SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10224048/" }, { "id": "PMID:15080793", "title": "InsP3-mediated intracellular calcium signalling is altered by expression of synaptojanin-1", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15080793/" } ], "x": 2660, "y": 1626.2534246575342, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676185", "label": "PI is phosphorylated to PI4P by PI4KA/2A/2B at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:19877" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676185", "summary": "PI is phosphorylated to PI4P by PI4KA/2A/2B at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI to ADP, PI4P in Golgi membrane · cytosol and cross-references RHEA:19877.", "equation": "ATP + PI → ADP + PI4P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1524146", "reactome_id": "R-ALL-1524146", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol 4-kinase activity of PI4KA/2A/2B [cytosol]", "activity_id": "1806241", "ec_numbers": [ "2.7.1.67" ], "accessions": [] } ], "citations": [ { "id": "PMID:10559940", "title": "ARF mediates recruitment of PtdIns-4-OH kinase-beta and stimulates synthesis of PtdIns(4,5)P2 on the Golgi complex", "journal": "Nat Cell Biol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10559940/" }, { "id": "PMID:11923287", "title": "Characterization of type II phosphatidylinositol 4-kinase isoforms reveals association of the enzymes with endosomal vesicular compartments", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11923287/" }, { "id": "PMID:11279162", "title": "Cloning of a human type II phosphatidylinositol 4-kinase reveals a novel lipid kinase family", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11279162/" }, { "id": "PMID:12324459", "title": "Type II phosphatidylinositol 4-kinase beta is a cytosolic and peripheral membrane protein that is recruited to the plasma membrane and activated by Rac-GTP", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12324459/" }, { "id": "PMID:10101268", "title": "Functional expression and characterisation of a new human phosphatidylinositol 4-kinase PI4K230", "journal": "Biochim Biophys Acta", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10101268/" } ], "x": 2938.666666666667, "y": 1626.2534246575342, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676203", "label": "PI(3,5)P2 is dephosphorylated to PI5P by SYNJ/MTMs at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:39019" ], "source_url": "https://reactome.org/content/detail/R-HSA-1676203", "summary": "PI(3,5)P2 is dephosphorylated to PI5P by SYNJ/MTMs at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(3,5)P2, H2O to PI5P, Pi in plasma membrane · cytosol and cross-references RHEA:39019.", "equation": "PI(3,5)P2 + H2O → PI5P + Pi", "inputs": [ { "node_id": "R-ALL-1806224", "reactome_id": "R-ALL-1806224", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1806240", "reactome_id": "R-ALL-1806240", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity of MTM1,MTMR1,MTMR3,MTMR6,MTMR14,SYNJ1,SYNJ2 [cytosol]", "activity_id": "1806177", "ec_numbers": [ "3.1.3.95" ], "accessions": [] } ], "citations": [ { "id": "PMID:10224048", "title": "SAC1-like domains of yeast SAC1, INP52, and INP53 and of human synaptojanin encode polyphosphoinositide phosphatases", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10224048/" }, { "id": "PMID:17651088", "title": "Myotubularin lipid phosphatase binds the hVPS15/hVPS34 lipid kinase complex on endosomes", "journal": "Traffic", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17651088/" }, { "id": "PMID:14660569", "title": "Production of phosphatidylinositol 5-phosphate by the phosphoinositide 3-phosphatase myotubularin in mammalian cells", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14660569/" }, { "id": "PMID:12646134", "title": "Phosphatidylinositol-5-phosphate activation and conserved substrate specificity of the myotubularin phosphatidylinositol 3-phosphatases", "journal": "Curr Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12646134/" }, { "id": "PMID:12118066", "title": "The PtdIns3P phosphatase myotubularin is a cytoplasmic protein that also localizes to Rac1-inducible plasma membrane ruffles", "journal": "J Cell Sci", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12118066/" }, { "id": "PMID:11676921", "title": "Characterization of MTMR3. an inositol lipid 3-phosphatase with novel substrate specificity", "journal": "Curr Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11676921/" } ], "x": 2381.3333333333335, "y": 1631.5821917808219, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676204", "label": "PI(3,4)P2 is dephosphorylated to PI4P by TPTE2 at the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676204", "summary": "PI(3,4)P2 is dephosphorylated to PI4P by TPTE2 at the Golgi membrane is a Reactome release 97 curated reaction event. The source record connects H2O, PI(3,4)P2 to PI4P, Pi in Golgi membrane · cytosol.", "equation": "H2O + PI(3,4)P2 → PI4P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806230", "reactome_id": "R-ALL-1806230", "label": "PI(3,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,4-bisphosphate 3-phosphatase activity of TPTE2-like proteins [Golgi membrane]", "activity_id": "1806220", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:14659893", "title": "The TPTE gene family: cellular expression, subcellular localization and alternative splicing", "journal": "Gene", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14659893/" }, { "id": "PMID:11716755", "title": "TPIP: a novel phosphoinositide 3-phosphatase", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11716755/" } ], "x": 2938.666666666667, "y": 1807.4315068493152, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1676206", "label": "PI4P is phosphorylated to PI(3,4)P2 by PIK3C2A at the early endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1676206", "summary": "PI4P is phosphorylated to PI(3,4)P2 by PIK3C2A at the early endosome membrane is a Reactome release 97 curated reaction event. The source record connects ATP, PI4P to ADP, PI(3,4)P2 in early endosome membrane · cytosol.", "equation": "ATP + PI4P → ADP + PI(3,4)P2", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806180", "reactome_id": "R-ALL-1806180", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806238", "reactome_id": "R-ALL-1806238", "label": "PI(3,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-4-phosphate 3-kinase activity of PIK3C2A:Ca2+/Mg2+ [cytosol]", "activity_id": "1806261", "ec_numbers": [ "2.7.1.154" ], "accessions": [] } ], "citations": [ { "id": "PMID:21081650", "title": "PI3KC2?, a class II PI3K, is required for dynamin-independent internalization pathways", "journal": "J Cell Sci", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21081650/" }, { "id": "PMID:10805725", "title": "Class II phosphoinositide 3-kinases are downstream targets of activated polypeptide growth factor receptors", "journal": "Mol Cell Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10805725/" } ], "x": 2381.3333333333335, "y": 1812.7602739726028, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1678650", "label": "IDS hydrolyses Heparan sulfate chain(5)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1678650", "summary": "IDS hydrolyses Heparan sulfate chain(5) is a Reactome release 97 curated reaction event. The source record connects H2O, IdoA2S-GlcNS3S- group to SO4(2-), IdoA-GlcNS3S- group in lysosomal lumen.", "equation": "H2O + IdoA2S-GlcNS3S- group → SO4(2-) + IdoA-GlcNS3S- group", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090080", "reactome_id": "R-ALL-2090080", "label": "IdoA2S-GlcNS3S- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63808", "formula": "C35H56NO39S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090055", "reactome_id": "R-ALL-2090055", "label": "IdoA-GlcNS3S- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63809", "formula": "C35H56NO36S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "iduronate-2-sulfatase activity of IDS dimer [lysosomal lumen]", "activity_id": "1678645", "ec_numbers": [ "3.1.6.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:2122463", "title": "Hunter syndrome: isolation of an iduronate-2-sulfatase cDNA clone and analysis of patient DNA", "journal": "Proc Natl Acad Sci U S A", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2122463/" } ], "x": 2660, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1678660", "label": "HGSNAT oligomer acetylates Heparan sulfate chain(3)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal membrane · cytosol · lysosomal lumen", "compartment_ids": [ "GO:0005765", "GO:0005829", "GO:0043202" ], "rhea_ids": [ "RHEA:15126" ], "source_url": "https://reactome.org/content/detail/R-HSA-1678660", "summary": "HGSNAT oligomer acetylates Heparan sulfate chain(3) is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, GlcN-IdoA2S-GlcNS3S- group to CoA-SH, GlcNAc-IdoA2S-GlcNS3S- group, H+ in lysosomal membrane · cytosol · lysosomal lumen and cross-references RHEA:15126.", "equation": "Ac-CoA + GlcN-IdoA2S-GlcNS3S- group → CoA-SH + GlcNAc-IdoA2S-GlcNS3S- group + H+", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090040", "reactome_id": "R-ALL-2090040", "label": "GlcN-IdoA2S-GlcNS3S- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63806", "formula": "C41H67N2O43S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090069", "reactome_id": "R-ALL-2090069", "label": "GlcNAc-IdoA2S-GlcNS3S- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63807", "formula": "C43H69N2O44S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428040", "reactome_id": "R-ALL-428040", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "heparan-alpha-glucosaminide N-acetyltransferase activity of HGSNAT oligomer [lysosomal membrane]", "activity_id": "1678732", "ec_numbers": [ "2.3.1.78" ], "accessions": [] } ], "citations": [ { "id": "PMID:20650889", "title": "Analysis of the biogenesis of heparan sulfate acetyl-CoA:alpha-glucosaminide N-acetyltransferase provides insights into the mechanism underlying its complete deficiency in mucopolysaccharidosis IIIC", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20650889/" }, { "id": "PMID:16960811", "title": "Identification of the gene encoding the enzyme deficient in mucopolysaccharidosis IIIC (Sanfilippo disease type C)", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16960811/" }, { "id": "PMID:17033958", "title": "Mutations in TMEM76* cause mucopolysaccharidosis IIIC (Sanfilippo C syndrome)", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17033958/" } ], "x": 2938.666666666667, "y": 771.469696969697, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1678694", "label": "Heparanase 2 (HPSE2) binds heparan sulfate proteoglycans", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1678694", "summary": "Heparanase 2 (HPSE2) binds heparan sulfate proteoglycans is a Reactome release 97 curated reaction event. The source record connects HS/HPIN-PGs, HPSE2(1-592) to HPSE2:HS/HPIN-PGs in plasma membrane · extracellular region.", "equation": "HS/HPIN-PGs + HPSE2(1-592) → HPSE2:HS/HPIN-PGs", "inputs": [ { "node_id": "R-HSA-2076639", "reactome_id": "R-HSA-2076639", "label": "HS/HPIN-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-1678680", "reactome_id": "R-HSA-1678680", "label": "HPSE2(1-592)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9774311", "reactome_id": "R-HSA-9774311", "label": "HPSE2:HS/HPIN-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein sequestering activity of HPSE2(1-592) [plasma membrane]", "activity_id": "9954174", "ec_numbers": null, "accessions": [ "Q8WWQ2" ] } ], "citations": [ { "id": "PMID:20560210", "title": "Mutations in HPSE2 cause urofacial syndrome", "journal": "Am J Hum Genet", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20560210/" }, { "id": "PMID:20576607", "title": "Heparanase 2 interacts with heparan sulfate with high affinity and inhibits heparanase activity", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20576607/" }, { "id": "PMID:11027606", "title": "Cloning and expression profiling of Hpa2, a novel mammalian heparanase family member", "journal": "Biochem Biophys Res Commun", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11027606/" }, { "id": "PMID:20560209", "title": "Loss-of-function mutations in HPSE2 cause the autosomal recessive urofacial syndrome", "journal": "Am J Hum Genet", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20560209/" } ], "x": 2938.666666666667, "y": 1030.8030303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1678708", "label": "SGSH hydrolyses Heparan sulfate chain(2)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1678708", "summary": "SGSH hydrolyses Heparan sulfate chain(2) is a Reactome release 97 curated reaction event. The source record connects H2O, GlcNS6S-IdoAS2-GlcNS3S- group to SO4(2-), GlcN-IdoA2S-GlcNS3S- group in lysosomal lumen.", "equation": "2 H2O + GlcNS6S-IdoAS2-GlcNS3S- group → 2 SO4(2-) + GlcN-IdoA2S-GlcNS3S- group", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090072", "reactome_id": "R-ALL-2090072", "label": "GlcNS6S-IdoAS2-GlcNS3S- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63805", "formula": "C41H67N2O49S5", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090040", "reactome_id": "R-ALL-2090040", "label": "GlcN-IdoA2S-GlcNS3S- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63806", "formula": "C41H67N2O43S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-sulfoglucosamine sulfohydrolase activity of SGSH:Ca2+ [lysosomal lumen]", "activity_id": "1678719", "ec_numbers": [ "3.10.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:7493035", "title": "Cloning of the sulphamidase gene and identification of mutations in Sanfilippo A syndrome", "journal": "Nat Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7493035/" }, { "id": "PMID:9285796", "title": "Novel mutations in Sanfilippo A syndrome: implications for enzyme function", "journal": "Hum Mol Genet", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9285796/" } ], "x": 2938.666666666667, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1678716", "label": "IDUA cleaves iduronate from HS chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1678716", "summary": "IDUA cleaves iduronate from HS chain is a Reactome release 97 curated reaction event. The source record connects H2O, IdoA-GlcN6S-IdoA2S-GlcN3S- group to aldehydo-L-iduronic acid, GlcNS6S-IdoAS2-GlcNS3S- group in lysosomal lumen.", "equation": "H2O + IdoA-GlcN6S-IdoA2S-GlcN3S- group → aldehydo-L-iduronic acid + GlcNS6S-IdoAS2-GlcNS3S- group", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090033", "reactome_id": "R-ALL-2090033", "label": "IdoA-GlcN6S-IdoA2S-GlcN3S- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747403", "formula": "C47H68N2O56S5", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1678617", "reactome_id": "R-ALL-1678617", "label": "aldehydo-L-iduronic acid", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:21338", "formula": "C6H9O7", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090072", "reactome_id": "R-ALL-2090072", "label": "GlcNS6S-IdoAS2-GlcNS3S- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63805", "formula": "C41H67N2O49S5", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-iduronidase activity of IDUA [lysosomal lumen]", "activity_id": "1678683", "ec_numbers": [ "3.2.1.76" ], "accessions": [ "P35475" ] } ], "citations": [ { "id": "PMID:1946389", "title": "Human alpha-L-iduronidase: cDNA isolation and expression", "journal": "Proc Natl Acad Sci U S A", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1946389/" }, { "id": "PMID:10466419", "title": "Mucopolysaccharidosis type I: characterization of novel mutations affecting alpha-L-iduronidase activity", "journal": "Clin Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10466419/" } ], "x": 2381.3333333333335, "y": 948.2878787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1678742", "label": "NAGLU hydrolyses Heparan sulfate chain(4)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1678742", "summary": "NAGLU hydrolyses Heparan sulfate chain(4) is a Reactome release 97 curated reaction event. The source record connects GlcNAc-IdoA2S-GlcNS3S- group, H2O to GlcNAc, IdoA2S-GlcNS3S- group in lysosomal lumen.", "equation": "GlcNAc-IdoA2S-GlcNS3S- group + H2O → GlcNAc + IdoA2S-GlcNS3S- group", "inputs": [ { "node_id": "R-ALL-2090069", "reactome_id": "R-ALL-2090069", "label": "GlcNAc-IdoA2S-GlcNS3S- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63807", "formula": "C43H69N2O44S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1678743", "reactome_id": "R-ALL-1678743", "label": "GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17411", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090080", "reactome_id": "R-ALL-2090080", "label": "IdoA2S-GlcNS3S- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63808", "formula": "C35H56NO39S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alpha-N-acetylglucosaminidase activity of NAGLU(59-743) [lysosomal lumen]", "activity_id": "1678739", "ec_numbers": [ "3.2.1.50" ], "accessions": [ "P54802" ] } ], "citations": [ { "id": "PMID:8776591", "title": "Cloning and expression of the gene involved in Sanfilippo B syndrome (mucopolysaccharidosis III B)", "journal": "Hum Mol Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8776591/" }, { "id": "PMID:16151907", "title": "Molecular defects in Sanfilippo syndrome type B (mucopolysaccharidosis IIIB)", "journal": "J Inherit Metab Dis", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16151907/" } ], "x": 2660, "y": 948.2878787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1678854", "label": "GUSB tetramer hydrolyses CS/HS precursor", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [ "RHEA:17634" ], "source_url": "https://reactome.org/content/detail/R-HSA-1678854", "summary": "GUSB tetramer hydrolyses CS/HS precursor is a Reactome release 97 curated reaction event. The source record connects CS/HS precursor, H2O to GlcA, linker chain(2) in lysosomal lumen and cross-references RHEA:17634.", "equation": "CS/HS precursor + H2O → GlcA + linker chain(2)", "inputs": [ { "node_id": "R-ALL-2065212", "reactome_id": "R-ALL-2065212", "label": "CS/HS precursor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747385", "formula": "C23H37O21", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1678868", "reactome_id": "R-ALL-1678868", "label": "GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4178", "formula": "C6H10O7", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090075", "reactome_id": "R-ALL-2090075", "label": "linker chain(2)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:154964", "formula": "C17H30O15", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-glucuronidase activity of GUSB tetramer [lysosomal lumen]", "activity_id": "1678848", "ec_numbers": [ "3.2.1.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:3468507", "title": "Cloning, sequencing, and expression of cDNA for human beta-glucuronidase", "journal": "Proc Natl Acad Sci U S A", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3468507/" }, { "id": "PMID:11568288", "title": "A novel inhibitor of beta-glucuronidase: L-aspartic acid", "journal": "Pediatr Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11568288/" }, { "id": "PMID:19224584", "title": "Mutations and polymorphisms in GUSB gene in mucopolysaccharidosis VII (Sly Syndrome)", "journal": "Hum Mutat", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19224584/" } ], "x": 2660, "y": 783.2575757575758, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-169260", "label": "Reducing equivalents from beta-oxidation of fatty acids transfer to ETF", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-169260", "summary": "Reducing equivalents from beta-oxidation of fatty acids transfer to ETF is a Reactome release 97 curated reaction event. The source record connects FADH2, ETF:FAD to ETF:FADH2, FAD in mitochondrial matrix.", "equation": "FADH2 + ETF:FAD → ETF:FADH2 + FAD", "inputs": [ { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-169267", "reactome_id": "R-HSA-169267", "label": "ETF:FAD", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-169268", "reactome_id": "R-HSA-169268", "label": "ETF:FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "electron transfer activity of ETF:FAD [mitochondrial matrix]", "activity_id": "169261", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10327278", "title": "Defects in mitochondrial beta-oxidation of fatty acids", "journal": "Curr. Opin. Lipidol.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10327278/" } ], "x": 5008.666666666667, "y": 832.7666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-169270", "label": "ETFDH oxidises ETF (reduced) to ETF, reduces CoQ to CoQH2", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:24053" ], "source_url": "https://reactome.org/content/detail/R-HSA-169270", "summary": "ETFDH oxidises ETF (reduced) to ETF, reduces CoQ to CoQH2 is a Reactome release 97 curated reaction event. The source record connects ETF:FADH2, CoQ to ETF:FAD, H+, CoQH2 in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:24053.", "equation": "ETF:FADH2 + CoQ → ETF:FAD + H+ + CoQH2", "inputs": [ { "node_id": "R-HSA-169268", "reactome_id": "R-HSA-169268", "label": "ETF:FADH2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-73663", "reactome_id": "R-ALL-73663", "label": "CoQ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46245", "formula": "C59H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-169267", "reactome_id": "R-HSA-169267", "label": "ETF:FAD", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2162198", "reactome_id": "R-ALL-2162198", "label": "CoQH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "electron-transferring-flavoprotein dehydrogenase activity of ETFDH [mitochondrial inner membrane]", "activity_id": "169271", "ec_numbers": [ "1.5.5.1" ], "accessions": [ "Q16134" ] } ], "citations": [ { "id": "PMID:1327877", "title": "Saturation kinetics of coenzyme Q in NADH and succinate oxidation in beef heart mitochondria", "journal": "FEBS Lett.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1327877/" }, { "id": "PMID:4288886", "title": "Studies on the mechanisms of oxidative phosphorylation. IX. Effect of cytochrome c on energy-linked processes", "journal": "J. Biol. Chem.", "year": 1966, "url": "https://pubmed.ncbi.nlm.nih.gov/4288886/" } ], "x": 4451.333333333333, "y": 832.7666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-170796", "label": "NPC transports GCK1:GCKR from cytosol to nucleoplasm", "schema_class": "BlackBoxEvent", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "nuclear envelope", "compartment_ids": [ "GO:0005635" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-170796", "summary": "NPC transports GCK1:GCKR from cytosol to nucleoplasm is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects GCK1:GCKR complex to GCK1:GCKR complex in nuclear envelope.", "equation": "GCK1:GCKR complex → GCK1:GCKR complex", "inputs": [ { "node_id": "R-HSA-170827", "reactome_id": "R-HSA-170827", "label": "GCK1:GCKR complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-170804", "reactome_id": "R-HSA-170804", "label": "GCK1:GCKR complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "structural constituent of nuclear pore activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "450099", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10601273", "title": "Nuclear import of hepatic glucokinase depends upon glucokinase regulatory protein, whereas export is due to a nuclear export signal sequence in glucokinase", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10601273/" } ], "x": 311.33333333333337, "y": 627.6875, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-170799", "label": "cytosolic GCK1:GKRP complex glucokinase (GCK1) + glucokinase regulatory protein (GKRP)", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-170799", "summary": "cytosolic GCK1:GKRP complex glucokinase (GCK1) + glucokinase regulatory protein (GKRP) is a Reactome release 97 curated reaction event. The source record connects GCK1:GCKR complex to GCK, GCKR in cytosol.", "equation": "GCK1:GCKR complex → GCK + GCKR", "inputs": [ { "node_id": "R-HSA-170827", "reactome_id": "R-HSA-170827", "label": "GCK1:GCKR complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-450094", "reactome_id": "R-HSA-450094", "label": "GCK", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-170818", "reactome_id": "R-HSA-170818", "label": "GCKR", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:14627435", "title": "Differences in regulatory properties between human and rat glucokinase regulatory protein", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14627435/" }, { "id": "PMID:10601273", "title": "Nuclear import of hepatic glucokinase depends upon glucokinase regulatory protein, whereas export is due to a nuclear export signal sequence in glucokinase", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10601273/" } ], "x": 868.6666666666667, "y": 579.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-170810", "label": "nucleoplasmic GCK1:GCKR complex =\u003e glucokinase (GCK1) + glucokinase regulatory protein (GCKR)", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-170810", "summary": "nucleoplasmic GCK1:GCKR complex =\u003e glucokinase (GCK1) + glucokinase regulatory protein (GCKR) is a Reactome release 97 curated reaction event. The source record connects GCK1:GCKR complex to GCK, GCKR in nucleoplasm.", "equation": "GCK1:GCKR complex → GCK + GCKR", "inputs": [ { "node_id": "R-HSA-170804", "reactome_id": "R-HSA-170804", "label": "GCK1:GCKR complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-450102", "reactome_id": "R-HSA-450102", "label": "GCK", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-170805", "reactome_id": "R-HSA-170805", "label": "GCKR", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "glucose sensor activity of GCK1:GCKR complex [nucleoplasm]", "activity_id": "9954132", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10601273", "title": "Nuclear import of hepatic glucokinase depends upon glucokinase regulatory protein, whereas export is due to a nuclear export signal sequence in glucokinase", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10601273/" } ], "x": 311.33333333333337, "y": 579.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-170824", "label": "glucokinase (GCK1) + glucokinase regulatory protein (GKRP) GCK1:GKRP complex", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-170824", "summary": "glucokinase (GCK1) + glucokinase regulatory protein (GKRP) GCK1:GKRP complex is a Reactome release 97 curated reaction event. The source record connects GCK, GCKR to GCK1:GCKR complex in cytosol.", "equation": "GCK + GCKR → GCK1:GCKR complex", "inputs": [ { "node_id": "R-HSA-450094", "reactome_id": "R-HSA-450094", "label": "GCK", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-170818", "reactome_id": "R-HSA-170818", "label": "GCKR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-170827", "reactome_id": "R-HSA-170827", "label": "GCK1:GCKR complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "kinase inhibitor activity of GCKR [cytosol]", "activity_id": "9954131", "ec_numbers": null, "accessions": [ "Q14397" ] } ], "citations": [ { "id": "PMID:14627435", "title": "Differences in regulatory properties between human and rat glucokinase regulatory protein", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14627435/" } ], "x": 590, "y": 579.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-170825", "label": "NPC exports GCK1 from nucleus", "schema_class": "BlackBoxEvent", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "nuclear envelope", "compartment_ids": [ "GO:0005635" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-170825", "summary": "NPC exports GCK1 from nucleus is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects GCK to GCK in nuclear envelope.", "equation": "GCK → GCK", "inputs": [ { "node_id": "R-HSA-450102", "reactome_id": "R-HSA-450102", "label": "GCK", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-450094", "reactome_id": "R-HSA-450094", "label": "GCK", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10601273", "title": "Nuclear import of hepatic glucokinase depends upon glucokinase regulatory protein, whereas export is due to a nuclear export signal sequence in glucokinase", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10601273/" } ], "x": 590, "y": 627.6875, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-173597", "label": "UDP-glucose is oxidised to UDP-glucuronate", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23597" ], "source_url": "https://reactome.org/content/detail/R-HSA-173597", "summary": "UDP-glucose is oxidised to UDP-glucuronate is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, NAD+, H2O to UDP-GlcA, NADH, H+ in cytosol and cross-references RHEA:23597.", "equation": "UDP-Glc + 2 NAD+ + H2O → UDP-GlcA + 2 NADH + 3 H+", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-158601", "reactome_id": "R-ALL-158601", "label": "UDP-GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "UDP-glucose 6-dehydrogenase activity of 6xUGDH [cytosol]", "activity_id": "173601", "ec_numbers": [ "1.1.1.22" ], "accessions": [] } ], "citations": [ { "id": "PMID:15044486", "title": "Characterization of human UDP-glucose dehydrogenase. CYS-276 is required for the second of two successive oxidations", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15044486/" } ], "x": 7461.333333333333, "y": 3476.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174367", "label": "GCL ligates L-Glu to L-Cys", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13286" ], "source_url": "https://reactome.org/content/detail/R-HSA-174367", "summary": "GCL ligates L-Glu to L-Cys is a Reactome release 97 curated reaction event. The source record connects L-Glu, L-Cys, ATP to gGluCys, ADP, Pi in cytosol and cross-references RHEA:13286.", "equation": "L-Glu + L-Cys + ATP → gGluCys + ADP + Pi", "inputs": [ { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-174393", "reactome_id": "R-ALL-174393", "label": "gGluCys", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58173", "formula": "C8H13N2O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutamate-cysteine ligase activity of GCL [cytosol]", "activity_id": "174398", "ec_numbers": [ "6.3.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:7826375", "title": "Cloning and sequencing of the cDNA for the light subunit of human liver gamma-glutamylcysteine synthetase and relative mRNA levels for heavy and light subunits in human normal tissues", "journal": "Biochem Biophys Res Commun", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7826375/" }, { "id": "PMID:9675072", "title": "Expression and purification of human gamma-glutamylcysteine synthetase", "journal": "Protein Expr Purif", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9675072/" } ], "x": 590, "y": 3479.8888888888887, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174368", "label": "SLC35D1 hexamer transports UDP-GlcA, UDP-GlcNAc from cytosol to endoplasmic reticulum lumen", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [ "RHEA:73703" ], "source_url": "https://reactome.org/content/detail/R-HSA-174368", "summary": "SLC35D1 hexamer transports UDP-GlcA, UDP-GlcNAc from cytosol to endoplasmic reticulum lumen is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects UDP-GlcA, UDP-GlcNAc to UDP-GlcA, UDP-GlcNAc in endoplasmic reticulum membrane and cross-references RHEA:73703.", "equation": "UDP-GlcA + UDP-GlcNAc → UDP-GlcA + UDP-GlcNAc", "inputs": [ { "node_id": "R-ALL-158601", "reactome_id": "R-ALL-158601", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-162756", "reactome_id": "R-ALL-162756", "label": "UDP-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-174384", "reactome_id": "R-ALL-174384", "label": "UDP-GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-3499326", "reactome_id": "R-ALL-3499326", "label": "UDP-GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "UDP-glucuronate transmembrane transporter activity of SLC35D1 hexamer [endoplasmic reticulum membrane]", "activity_id": "174365", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11322953", "title": "Molecular characterization of human UDP-glucuronic acid/UDP-N-acetylgalactosamine transporter, a novel nucleotide sugar transporter with dual substrate specificity", "journal": "FEBS Lett", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11322953/" } ], "x": 7740, "y": 2883.0882352941176, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-174374", "label": "MTR transfers CH3 from MeCbl to HCYS", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "phase-two", "sulfur-amino-acids", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-174374", "summary": "MTR transfers CH3 from MeCbl to HCYS is a Reactome release 97 curated reaction event. The source record connects MTRR:MTR:MeCbl, HCYS to MTRR:MTR:cob(I)alamin, L-Met in cytosol.", "equation": "MTRR:MTR:MeCbl + HCYS → MTRR:MTR:cob(I)alamin + L-Met", "inputs": [ { "node_id": "R-HSA-3149551", "reactome_id": "R-HSA-3149551", "label": "MTRR:MTR:MeCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-174369", "reactome_id": "R-ALL-174369", "label": "HCYS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58199", "formula": "C4H9NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3149516", "reactome_id": "R-HSA-3149516", "label": "MTRR:MTR:cob(I)alamin", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-174390", "reactome_id": "R-ALL-174390", "label": "L-Met", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57844", "formula": "C5H11NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methionine synthase activity of MTRR:MTR:MeCbl [cytosol]", "activity_id": "174360", "ec_numbers": [ "2.1.1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:10978155", "title": "Interaction of flavodoxin with cobalamin-dependent methionine synthase", "journal": "Biochemistry", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10978155/" }, { "id": "PMID:8968737", "title": "Human methionine synthase: cDNA cloning and identification of mutations in patients of the cblG complementation group of folate/cobalamin disorders", "journal": "Hum Mol Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8968737/" } ], "x": 1530, "y": 3022.777777777778, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174389", "label": "PAPSS1,2 transfer PO4(2-) group from ATP to APS to form PAPS", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24153" ], "source_url": "https://reactome.org/content/detail/R-HSA-174389", "summary": "PAPSS1,2 transfer PO4(2-) group from ATP to APS to form PAPS is a Reactome release 97 curated reaction event. The source record connects ATP, APS to ADP, PAPS, H+ in cytosol and cross-references RHEA:24153.", "equation": "ATP + APS → ADP + PAPS + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-174370", "reactome_id": "R-ALL-174370", "label": "APS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58243", "formula": "C10H12N5O10PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "adenylylsulfate kinase activity of PAPSS1,2 [cytosol]", "activity_id": "174366", "ec_numbers": [ "2.7.1.25" ], "accessions": [] } ], "citations": [ { "id": "PMID:9771708", "title": "Mutations in orthologous genes in human spondyloepimetaphyseal dysplasia and the brachymorphic mouse", "journal": "Nat Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9771708/" }, { "id": "PMID:9576487", "title": "Sulfation in high endothelial venules: cloning and expression of the human PAPS synthetase", "journal": "FASEB J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9576487/" }, { "id": "PMID:9668121", "title": "Molecular cloning, expression, and characterization of human bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase and its functional domains", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9668121/" } ], "x": 2660, "y": 747.8939393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174391", "label": "MAT1A multimers transfer Ado from ATP to L-Met", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "phase-two", "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21081" ], "source_url": "https://reactome.org/content/detail/R-HSA-174391", "summary": "MAT1A multimers transfer Ado from ATP to L-Met is a Reactome release 97 curated reaction event. The source record connects ATP, L-Met, H2O to AdoMet, Pi, PPi in cytosol and cross-references RHEA:21081.", "equation": "ATP + L-Met + H2O → AdoMet + Pi + PPi", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-174390", "reactome_id": "R-ALL-174390", "label": "L-Met", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57844", "formula": "C5H11NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methionine adenosyltransferase activity of MAT1A multimers [cytosol]", "activity_id": "174380", "ec_numbers": [ "2.5.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:12163696", "title": "Regulation of mammalian liver methionine adenosyltransferase", "journal": "J Nutr", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12163696/" }, { "id": "PMID:9175157", "title": "S-adenosylmethionine synthesis: molecular mechanisms and clinical implications", "journal": "Pharmacol Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9175157/" } ], "x": 1251.3333333333333, "y": 2831.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174392", "label": "PAPSS1,2 transfer SO4(2-) group to ATP to form APS", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18134" ], "source_url": "https://reactome.org/content/detail/R-HSA-174392", "summary": "PAPSS1,2 transfer SO4(2-) group to ATP to form APS is a Reactome release 97 curated reaction event. The source record connects ATP, SO4(2-), H+ to APS, PPi in cytosol and cross-references RHEA:18134.", "equation": "ATP + SO4(2-) + H+ → APS + PPi", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-174375", "reactome_id": "R-ALL-174375", "label": "SO4(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-174370", "reactome_id": "R-ALL-174370", "label": "APS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58243", "formula": "C10H12N5O10PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfate adenylyltransferase (ATP) activity of PAPSS1,2 [cytosol]", "activity_id": "174381", "ec_numbers": [ "2.7.7.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:9576487", "title": "Sulfation in high endothelial venules: cloning and expression of the human PAPS synthetase", "journal": "FASEB J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9576487/" }, { "id": "PMID:9771708", "title": "Mutations in orthologous genes in human spondyloepimetaphyseal dysplasia and the brachymorphic mouse", "journal": "Nat Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9771708/" }, { "id": "PMID:9668121", "title": "Molecular cloning, expression, and characterization of human bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase and its functional domains", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9668121/" } ], "x": 2938.666666666667, "y": 747.8939393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174394", "label": "GSS:Mg2+ dimer synthesizes GSH", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13558" ], "source_url": "https://reactome.org/content/detail/R-HSA-174394", "summary": "GSS:Mg2+ dimer synthesizes GSH is a Reactome release 97 curated reaction event. The source record connects gGluCys, Gly, ATP to GSH, ADP, Pi in cytosol and cross-references RHEA:13558.", "equation": "gGluCys + Gly + ATP → GSH + ADP + Pi", "inputs": [ { "node_id": "R-ALL-174393", "reactome_id": "R-ALL-174393", "label": "gGluCys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58173", "formula": "C8H13N2O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione synthase activity of GSS:Mg2+ dimer [cytosol]", "activity_id": "174399", "ec_numbers": [ "6.3.2.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:7646467", "title": "Sequencing and expression of a cDNA for human glutathione synthetase", "journal": "Biochem J", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7646467/" }, { "id": "PMID:10369661", "title": "Molecular basis of glutathione synthetase deficiency and a rare gene permutation event", "journal": "EMBO J.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10369661/" } ], "x": 868.6666666666667, "y": 3479.8888888888887, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174401", "label": "AHCY:NAD+ tetramer hydrolyses AdoHcy", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "phase-two", "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21709" ], "source_url": "https://reactome.org/content/detail/R-HSA-174401", "summary": "AHCY:NAD+ tetramer hydrolyses AdoHcy is a Reactome release 97 curated reaction event. The source record connects AdoHcy, H2O to HCYS, Ade-Rib in cytosol and cross-references RHEA:21709.", "equation": "AdoHcy + H2O → HCYS + Ade-Rib", "inputs": [ { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-174369", "reactome_id": "R-ALL-174369", "label": "HCYS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58199", "formula": "C4H9NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-83909", "reactome_id": "R-ALL-83909", "label": "Ade-Rib", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16335", "formula": "C10H13N5O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "adenosylhomocysteinase activity of AHCY:NAD+ tetramer [cytosol]", "activity_id": "174397", "ec_numbers": [ "3.13.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9586999", "title": "Structure determination of selenomethionyl S-adenosylhomocysteine hydrolase using data at a single wavelength", "journal": "Nat. Struct. Biol.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9586999/" }, { "id": "PMID:12590576", "title": "Catalytic strategy of S-adenosyl-L-homocysteine hydrolase: transition-state stabilization and the avoidance of abortive reactions", "journal": "Biochemistry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12590576/" } ], "x": 1251.3333333333333, "y": 2927.222222222222, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174916", "label": "Formation of N-glucuronides", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-174916", "summary": "Formation of N-glucuronides is a Reactome release 97 curated reaction event. The source record connects UDP-GlcA, N-centre functional group substrate to UDP, N-glucuronide in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "UDP-GlcA + N-centre functional group substrate → UDP + N-glucuronide", "inputs": [ { "node_id": "R-ALL-174384", "reactome_id": "R-ALL-174384", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174932", "reactome_id": "R-ALL-174932", "label": "N-centre functional group substrate", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158602", "reactome_id": "R-ALL-158602", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174918", "reactome_id": "R-ALL-174918", "label": "N-glucuronide", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "glucuronosyltransferase activity of UGT1A3,A4,A9 [endoplasmic reticulum membrane]", "activity_id": "174934", "ec_numbers": [ "2.4.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:12433823", "title": "Characterization of nicotine and cotinine N-glucuronidations in human liver microsomes", "journal": "Drug Metab Dispos", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12433823/" }, { "id": "PMID:14570768", "title": "N-glucuronidation of nicotine and cotinine by human liver microsomes and heterologously expressed UDP-glucuronosyltransferases", "journal": "Drug Metab Dispos", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14570768/" }, { "id": "PMID:15135306", "title": "Quaternary ammonium-linked glucuronidation of tamoxifen by human liver microsomes and UDP-glucuronosyltransferase 1A4", "journal": "Biochem Pharmacol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15135306/" } ], "x": 7461.333333333333, "y": 3127.1666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174931", "label": "UGTs transfer GlcA from UDP-GlcA to O-centre substrates", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-174931", "summary": "UGTs transfer GlcA from UDP-GlcA to O-centre substrates is a Reactome release 97 curated reaction event. The source record connects O-centre functional group substrate, UDP-GlcA to O-glucuronide, UDP in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "O-centre functional group substrate + UDP-GlcA → O-glucuronide + UDP", "inputs": [ { "node_id": "R-ALL-174911", "reactome_id": "R-ALL-174911", "label": "O-centre functional group substrate", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-174384", "reactome_id": "R-ALL-174384", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-174914", "reactome_id": "R-ALL-174914", "label": "O-glucuronide", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-158602", "reactome_id": "R-ALL-158602", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucuronosyltransferase activity of UGTs (O-GlcA forming) [endoplasmic reticulum membrane]", "activity_id": "174915", "ec_numbers": [ "2.4.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:8687483", "title": "Glucuronide conjugates of 4-aminobiphenyl and its N-hydroxy metabolites. pH stability and synthesis by human and dog liver.", "journal": "Biochem Pharmacol", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8687483/" }, { "id": "PMID:16258079", "title": "Glucuronidation of the aspirin metabolite salicylic acid by expressed UDP-glucuronosyltransferases and human liver microsomes", "journal": "Drug Metab Dispos", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16258079/" } ], "x": 7740, "y": 3127.1666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174959", "label": "The acetyl group from acetyl-CoA is transferred to NAT1", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-174959", "summary": "The acetyl group from acetyl-CoA is transferred to NAT1 is a Reactome release 97 curated reaction event. The source record connects NAT1, Ac-CoA to AcC-NAT1, CoA-SH in cytosol.", "equation": "NAT1 + Ac-CoA → AcC-NAT1 + CoA-SH", "inputs": [ { "node_id": "R-HSA-174956", "reactome_id": "R-HSA-174956", "label": "NAT1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-174972", "reactome_id": "R-HSA-174972", "label": "AcC-NAT1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein-cysteine S-acyltransferase activity of NAT1 [cytosol]", "activity_id": "9954138", "ec_numbers": [ "2.3.1" ], "accessions": [ "P18440" ] } ], "citations": [ { "id": "PMID:1559981", "title": "Site-directed mutagenesis of recombinant human arylamine N-acetyltransferase expressed in Escherichia coli. Evidence for direct involvement of Cys68 in the catalytic mechanism of polymorphic human NAT2.", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1559981/" } ], "x": 8018.666666666667, "y": 3127.1666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174963", "label": "NAT1 acetylation", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-174963", "summary": "NAT1 acetylation is a Reactome release 97 curated reaction event. The source record connects NAT1 substrate, AcC-NAT1 to NAT1 acetylated conjugate, NAT1 in cytosol.", "equation": "NAT1 substrate + AcC-NAT1 → NAT1 acetylated conjugate + NAT1", "inputs": [ { "node_id": "R-ALL-174969", "reactome_id": "R-ALL-174969", "label": "NAT1 substrate", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-174972", "reactome_id": "R-HSA-174972", "label": "AcC-NAT1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-174960", "reactome_id": "R-ALL-174960", "label": "NAT1 acetylated conjugate", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-174956", "reactome_id": "R-HSA-174956", "label": "NAT1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "arylamine N-acetyltransferase activity of AcC-NAT1 [cytosol]", "activity_id": "174964", "ec_numbers": [ "2.3.1.5" ], "accessions": [ "P18440" ] } ], "citations": [ { "id": "PMID:28380339", "title": "Molecular Basis of Substrate Specific Acetylation by N-Terminal Acetyltransferase NatB", "journal": "Structure", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28380339/" } ], "x": 7461.333333333333, "y": 3157.676470588235, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-174967", "label": "NAT2 acetylation", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-174967", "summary": "NAT2 acetylation is a Reactome release 97 curated reaction event. The source record connects NAT2 substrate, AcC-NAT2 to NAT2 acetylated conjugate, NAT2 in cytosol.", "equation": "NAT2 substrate + AcC-NAT2 → NAT2 acetylated conjugate + NAT2", "inputs": [ { "node_id": "R-ALL-174971", "reactome_id": "R-ALL-174971", "label": "NAT2 substrate", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-158833", "reactome_id": "R-HSA-158833", "label": "AcC-NAT2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-174958", "reactome_id": "R-ALL-174958", "label": "NAT2 acetylated conjugate", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-158692", "reactome_id": "R-HSA-158692", "label": "NAT2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "arylamine N-acetyltransferase activity of AcC-NAT2 [cytosol]", "activity_id": "158690", "ec_numbers": [ "2.3.1.5" ], "accessions": [ "P11245" ] } ], "citations": [ { "id": "PMID:8353847", "title": "Metabolic activation and deactivation of arylamine carcinogens by recombinant human NAT1 and polymorphic NAT2 acetyltransferases", "journal": "Carcinogenesis", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8353847/" }, { "id": "PMID:8068562", "title": "N-acetyltransferases, O-acetyltransferases, and N,O-acetyltransferases: enzymology and bioactivation", "journal": "Adv. Pharmacol.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8068562/" }, { "id": "PMID:23517104", "title": "Arylamine N-acetyltransferases: a structural perspective", "journal": "Br. J. Pharmacol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23517104/" } ], "x": 7740, "y": 3157.676470588235, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-175976", "label": "TMT transfers CH3 from AdoMet to BME", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-175976", "summary": "TMT transfers CH3 from AdoMet to BME is a Reactome release 97 curated reaction event. The source record connects BME, AdoMet to MMETOH, AdoHcy in cytosol.", "equation": "BME + AdoMet → MMETOH + AdoHcy", "inputs": [ { "node_id": "R-ALL-175981", "reactome_id": "R-ALL-175981", "label": "BME", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:41218", "formula": "C2H6OS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-175980", "reactome_id": "R-ALL-175980", "label": "MMETOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63861", "formula": "C3H8OS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "thiol S-methyltransferase activity of TMT [cytosol]", "activity_id": "175985", "ec_numbers": [ "2.1.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:8093089", "title": "Sequential methylation of 2-mercaptoethanol to the dimethyl sulfonium ion, 2-(dimethylthio)ethanol, in vivo and in vitro", "journal": "Biochem. Pharmacol.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8093089/" } ], "x": 8018.666666666667, "y": 3073.774509803922, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-175983", "label": "COMT transfer CH3 from AdoMet to 3,4DHBNZ", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-175983", "summary": "COMT transfer CH3 from AdoMet to 3,4DHBNZ is a Reactome release 97 curated reaction event. The source record connects 3,4DHBNZ, AdoMet to AdoHcy, 3H,4MBNZ in cytosol.", "equation": "3,4DHBNZ + AdoMet → AdoHcy + 3H,4MBNZ", "inputs": [ { "node_id": "R-ALL-175977", "reactome_id": "R-ALL-175977", "label": "3,4DHBNZ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36062", "formula": "C7H6O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-175978", "reactome_id": "R-ALL-175978", "label": "3H,4MBNZ", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63797", "formula": "C8H7O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "catechol O-methyltransferase activity of COMT [cytosol]", "activity_id": "175988", "ec_numbers": [ "2.1.1.6" ], "accessions": [ "P21964" ] } ], "citations": [ { "id": "PMID:8968737", "title": "Human methionine synthase: cDNA cloning and identification of mutations in patients of the cblG complementation group of folate/cobalamin disorders", "journal": "Hum Mol Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8968737/" } ], "x": 7461.333333333333, "y": 3081.401960784314, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-175987", "label": "NNMT transfers CH3 from AdoMet to PY", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16894" ], "source_url": "https://reactome.org/content/detail/R-HSA-175987", "summary": "NNMT transfers CH3 from AdoMet to PY is a Reactome release 97 curated reaction event. The source record connects PY, AdoMet to AdoHcy, MP+ in cytosol and cross-references RHEA:16894.", "equation": "PY + AdoMet → AdoHcy + MP+", "inputs": [ { "node_id": "R-ALL-175973", "reactome_id": "R-ALL-175973", "label": "PY", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16227", "formula": "C5H5N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-175979", "reactome_id": "R-ALL-175979", "label": "MP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15761", "formula": "C6H8N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pyridine N-methyltransferase activity of NNMT [cytosol]", "activity_id": "175975", "ec_numbers": [ "2.1.1.87" ], "accessions": [ "P40261" ] } ], "citations": [ { "id": "PMID:27570878", "title": "A Rapid and Efficient Assay for the Characterization of Substrates and Inhibitors of Nicotinamide N-Methyltransferase", "journal": "Biochemistry", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27570878/" } ], "x": 8018.666666666667, "y": 2883.0882352941176, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-175993", "label": "HNMT transfers CH3 group from AdoMet to Hist", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19302" ], "source_url": "https://reactome.org/content/detail/R-HSA-175993", "summary": "HNMT transfers CH3 group from AdoMet to Hist is a Reactome release 97 curated reaction event. The source record connects Hist, AdoMet to AdoHcy, MeHist, H+ in cytosol and cross-references RHEA:19302.", "equation": "Hist + AdoMet → AdoHcy + MeHist + H+", "inputs": [ { "node_id": "R-ALL-175992", "reactome_id": "R-ALL-175992", "label": "Hist", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58432", "formula": "C5H10N3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-175991", "reactome_id": "R-ALL-175991", "label": "MeHist", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58600", "formula": "C6H12N3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "histamine N-methyltransferase activity of HNMT [cytosol]", "activity_id": "175990", "ec_numbers": [ "2.1.1.8" ], "accessions": [ "P50135" ] } ], "citations": [ { "id": "PMID:7943261", "title": "Structure and function of human histamine N-methyltransferase: critical enzyme in histamine metabolism in airway", "journal": "Am. J. Physiol.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7943261/" }, { "id": "PMID:11566133", "title": "Two polymorphic forms of human histamine methyltransferase: structural, thermal, and kinetic comparisons", "journal": "Structure", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11566133/" }, { "id": "PMID:18154359", "title": "The histamine N-methyltransferase T105I polymorphism affects active site structure and dynamics", "journal": "Biochemistry", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18154359/" } ], "x": 7740, "y": 2030.6875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176054", "label": "GST dimers conjugate GSH with cytosolic substrates", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-176054", "summary": "GST dimers conjugate GSH with cytosolic substrates is a Reactome release 97 curated reaction event. The source record connects GSH, Electrophilic substrate to electrophilic substrate:SG in cytosol.", "equation": "GSH + Electrophilic substrate → electrophilic substrate:SG", "inputs": [ { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176070", "reactome_id": "R-ALL-176070", "label": "Electrophilic substrate", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-176049", "reactome_id": "R-ALL-176049", "label": "electrophilic substrate:SG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "glutathione transferase activity of GST dimers [cytosol]", "activity_id": "176052", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:8331657", "title": "Structure determination and refinement of human alpha class glutathione transferase A1-1, and a comparison with the Mu and Pi class enzymes", "journal": "J. Mol. Biol.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8331657/" }, { "id": "PMID:12211029", "title": "1.3-A resolution structure of human glutathione S-transferase with S-hexyl glutathione bound reveals possible extended ligandin binding site", "journal": "Proteins", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12211029/" }, { "id": "PMID:8431482", "title": "Characterization of two novel subunits of the alpha-class glutathione S-transferases of human liver", "journal": "Biochim. Biophys. Acta", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8431482/" }, { "id": "PMID:9839448", "title": "Purification and characterization of a novel alpha-class glutathione transferase from human liver", "journal": "Int. J. Biochem. Cell Biol.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9839448/" }, { "id": "PMID:20083122", "title": "Structural basis for featuring of steroid isomerase activity in alpha class glutathione transferases", "journal": "J. Mol. Biol.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20083122/" }, { "id": "PMID:10329152", "title": "Human glutathione transferase A4-4 crystal structures and mutagenesis reveal the basis of high catalytic efficiency with toxic lipid peroxidation products", "journal": "J. Mol. Biol.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10329152/" } ], "x": 8018.666666666667, "y": 3134.794117647059, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176059", "label": "GST trimers transfer GS from GSH to luminal substrates", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-176059", "summary": "GST trimers transfer GS from GSH to luminal substrates is a Reactome release 97 curated reaction event. The source record connects electrophilic substrate, GSH to electrophilic substrate:SG in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "electrophilic substrate + GSH → electrophilic substrate:SG", "inputs": [ { "node_id": "R-ALL-176065", "reactome_id": "R-ALL-176065", "label": "electrophilic substrate", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-158543", "reactome_id": "R-ALL-158543", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16856", "formula": "C10H17N3O6S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-176048", "reactome_id": "R-ALL-176048", "label": "electrophilic substrate:SG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "glutathione transferase activity of MGST trimers [endoplasmic reticulum membrane]", "activity_id": "158544", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:3372534", "title": "Gene expression of rat and human microsomal glutathione S-transferases", "journal": "J. Biol. Chem.", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3372534/" }, { "id": "PMID:8812420", "title": "Structural organization of the human microsomal glutathione S-transferase gene (GST12)", "journal": "Genomics", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8812420/" }, { "id": "PMID:8703034", "title": "Identification and characterization of a novel human microsomal glutathione S-transferase with leukotriene C4 synthase activity and significant sequence identity to 5-lipoxygenase-activating protein and leukotriene C4 synthase", "journal": "J. Biol. Chem.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8703034/" }, { "id": "PMID:9278457", "title": "Identification and characterization of a novel microsomal enzyme with glutathione-dependent transferase and peroxidase activities", "journal": "J. Biol. Chem.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9278457/" }, { "id": "PMID:11904223", "title": "The 3-D structure of microsomal glutathione transferase 1 at 6 A resolution as determined by electron crystallography of p22(1)2(1) crystals", "journal": "Biochim. Biophys. Acta", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11904223/" } ], "x": 7461.333333333333, "y": 3142.421568627451, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176474", "label": "3,3'-diiodothyronine + PAPS =\u003e 3,3'-diiodothyronine 4-sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-176474", "summary": "3,3'-diiodothyronine + PAPS =\u003e 3,3'-diiodothyronine 4-sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects 3,3'-diiodothyronine, PAPS to 3,3'-diiodothyronine 4-sulfate, PAP in cytosol.", "equation": "3,3'-diiodothyronine + PAPS → 3,3'-diiodothyronine 4-sulfate + PAP", "inputs": [ { "node_id": "R-ALL-176486", "reactome_id": "R-ALL-176486", "label": "3,3'-diiodothyronine", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35430", "formula": "C15H13I2NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-176519", "reactome_id": "R-ALL-176519", "label": "3,3'-diiodothyronine 4-sulfate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35431", "formula": "C15H13I2NO7S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aryl sulfotransferase activity of SULT dimers (T2) [cytosol]", "activity_id": "176678", "ec_numbers": [ "2.8.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10199779", "title": "Characterization of human iodothyronine sulfotransferases", "journal": "J Clin Endocrinol Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10199779/" }, { "id": "PMID:11739018", "title": "Characterization of human liver thermostable phenol sulfotransferase (SULT1A1) allozymes with 3,3',5-triiodothyronine as the substrate", "journal": "J Endocrinol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11739018/" }, { "id": "PMID:9463486", "title": "Expression and characterization of a novel thyroid hormone-sulfating form of cytosolic sulfotransferase from human liver", "journal": "Mol Pharmacol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9463486/" }, { "id": "PMID:11077054", "title": "Sulfation of iodothyronines by human sulfotransferase 1C1 (SULT1C1)*", "journal": "Biochem Pharmacol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11077054/" }, { "id": "PMID:10512730", "title": "Sulfation of iodothyronines by recombinant human liver steroid sulfotransferases", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10512730/" } ], "x": 7740, "y": 3081.401960784314, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176494", "label": "SULTs transfer (SO4)2- group to 27HCHOL", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-176494", "summary": "SULTs transfer (SO4)2- group to 27HCHOL is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 27HCHOL, PAPS to 27HCHOL3S, PAP in cytosol · endoplasmic reticulum membrane.", "equation": "27HCHOL + PAPS → 27HCHOL3S + PAP", "inputs": [ { "node_id": "R-ALL-176617", "reactome_id": "R-ALL-176617", "label": "27HCHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17703", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-176502", "reactome_id": "R-ALL-176502", "label": "27HCHOL3S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35419", "formula": "C27H46O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol sulfotransferase activity of SULTs active on 27HCHOL [cytosol]", "activity_id": "176537", "ec_numbers": [ "2.8.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11416019", "title": "Cholesterol and hydroxycholesterol sulfotransferases: identification, distinction from dehydroepiandrosterone sulfotransferase, and differential tissue expression", "journal": "Endocrinology", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11416019/" } ], "x": 8018.666666666667, "y": 3081.401960784314, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-176517", "label": "SULTs transfer (SO4)2- group to PREG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:52357" ], "source_url": "https://reactome.org/content/detail/R-HSA-176517", "summary": "SULTs transfer (SO4)2- group to PREG is a Reactome release 97 curated reaction event. The source record connects PREG, PAPS to PREGS, PAP in cytosol and cross-references RHEA:52357.", "equation": "PREG + PAPS → PREGS + PAP", "inputs": [ { "node_id": "R-ALL-193103", "reactome_id": "R-ALL-193103", "label": "PREG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16581", "formula": "C21H32O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-176665", "reactome_id": "R-ALL-176665", "label": "PREGS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35420", "formula": "C21H32O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid sulfotransferase activity of SULTs active on pregnenolone [cytosol]", "activity_id": "176472", "ec_numbers": [ "2.8.2.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:11457664", "title": "Expression and characterization of the human 3 beta-hydroxysteroid sulfotransferases (SULT2B1a and SULT2B1b)", "journal": "J Steroid Biochem Mol Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11457664/" }, { "id": "PMID:12145317", "title": "Mutational analysis of human hydroxysteroid sulfotransferase SULT2B1 isoforms reveals that exon 1B of the SULT2B1 gene produces cholesterol sulfotransferase, whereas exon 1A yields pregnenolone sulfotransferase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12145317/" } ], "x": 7461.333333333333, "y": 2890.7156862745096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176521", "label": "beta-estradiol + PAPS =\u003e beta-estradiol 3-sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:52373" ], "source_url": "https://reactome.org/content/detail/R-HSA-176521", "summary": "beta-estradiol + PAPS =\u003e beta-estradiol 3-sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects PAPS, EST17b to PAP, E2-SO4 in cytosol and cross-references RHEA:52373.", "equation": "PAPS + EST17b → PAP + E2-SO4", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193132", "reactome_id": "R-ALL-193132", "label": "EST17b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16469", "formula": "C18H24O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176492", "reactome_id": "R-ALL-176492", "label": "E2-SO4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4866", "formula": "C18H24O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid sulfotransferase activity of SULT1E1 dimer [cytosol]", "activity_id": "176571", "ec_numbers": [ "2.8.2.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:7779757", "title": "Bacterial expression and characterization of a cDNA for human liver estrogen sulfotransferase", "journal": "J Steroid Biochem Mol Biol", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7779757/" } ], "x": 7740, "y": 2890.7156862745096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176585", "label": "3,5,3'-triiodothyronine + PAPS =\u003e 3,5,3'-triiodothyronine 4-sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-176585", "summary": "3,5,3'-triiodothyronine + PAPS =\u003e 3,5,3'-triiodothyronine 4-sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects T3, PAPS to 3,5,3'-triiodothyronine 4-sulfate, PAP in cytosol.", "equation": "T3 + PAPS → 3,5,3'-triiodothyronine 4-sulfate + PAP", "inputs": [ { "node_id": "R-ALL-176667", "reactome_id": "R-ALL-176667", "label": "T3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:533015", "formula": "C15H12I3NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-176564", "reactome_id": "R-ALL-176564", "label": "3,5,3'-triiodothyronine 4-sulfate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35432", "formula": "C15H12I3NO7S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aryl sulfotransferase activity of SULT dimers (T3) [cytosol]", "activity_id": "176524", "ec_numbers": [ "2.8.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10199779", "title": "Characterization of human iodothyronine sulfotransferases", "journal": "J Clin Endocrinol Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10199779/" }, { "id": "PMID:11739018", "title": "Characterization of human liver thermostable phenol sulfotransferase (SULT1A1) allozymes with 3,3',5-triiodothyronine as the substrate", "journal": "J Endocrinol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11739018/" }, { "id": "PMID:9463486", "title": "Expression and characterization of a novel thyroid hormone-sulfating form of cytosolic sulfotransferase from human liver", "journal": "Mol Pharmacol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9463486/" }, { "id": "PMID:12039030", "title": "Highly conserved mouse and human brain sulfotransferases: molecular cloning, expression, and functional characterization", "journal": "Gene", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12039030/" }, { "id": "PMID:11077054", "title": "Sulfation of iodothyronines by human sulfotransferase 1C1 (SULT1C1)*", "journal": "Biochem Pharmacol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11077054/" }, { "id": "PMID:10512730", "title": "Sulfation of iodothyronines by recombinant human liver steroid sulfotransferases", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10512730/" } ], "x": 7461.333333333333, "y": 3089.029411764706, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176588", "label": "lithocholate + PAPS =\u003e lithocholate sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:51065" ], "source_url": "https://reactome.org/content/detail/R-HSA-176588", "summary": "lithocholate + PAPS =\u003e lithocholate sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects PAPS, LCA to PAP, lithocholate sulfate in cytosol and cross-references RHEA:51065.", "equation": "PAPS + LCA → PAP + lithocholate sulfate", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176655", "reactome_id": "R-ALL-176655", "label": "LCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29744", "formula": "C24H39O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176555", "reactome_id": "R-ALL-176555", "label": "lithocholate sulfate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133940", "formula": "C24H38O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfotransferase activity of SULT2A1 dimer [cytosol]", "activity_id": "176547", "ec_numbers": [ "2.8.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:2268288", "title": "Human liver steroid sulphotransferase sulphates bile acids", "journal": "Biochem J", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2268288/" } ], "x": 8018.666666666667, "y": 2890.7156862745096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176604", "label": "taurolithocholate + PAPS =\u003e taurolithocholate sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14014" ], "source_url": "https://reactome.org/content/detail/R-HSA-176604", "summary": "taurolithocholate + PAPS =\u003e taurolithocholate sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects PAPS, taurolithocholate to PAP, taurolithocholate sulfate in cytosol and cross-references RHEA:14014.", "equation": "PAPS + taurolithocholate → PAP + taurolithocholate sulfate", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176565", "reactome_id": "R-ALL-176565", "label": "taurolithocholate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17179", "formula": "C26H44NO5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176483", "reactome_id": "R-ALL-176483", "label": "taurolithocholate sulfate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17864", "formula": "C26H45NO8S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfotransferase activity of SULT2A1 dimer [cytosol]", "activity_id": "176547", "ec_numbers": [ "2.8.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:2268288", "title": "Human liver steroid sulphotransferase sulphates bile acids", "journal": "Biochem J", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2268288/" } ], "x": 7461.333333333333, "y": 2898.343137254902, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176606", "label": "Adenosine 3',5'-bisphosphate (PAP) + H2O =\u003e AMP + orthophosphate", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10041" ], "source_url": "https://reactome.org/content/detail/R-HSA-176606", "summary": "Adenosine 3',5'-bisphosphate (PAP) + H2O =\u003e AMP + orthophosphate is a Reactome release 97 curated reaction event. The source record connects PAP, H2O to AMP, Pi in cytosol and cross-references RHEA:10041.", "equation": "PAP + H2O → AMP + Pi", "inputs": [ { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3'(2'),5'-bisphosphate nucleotidase activity of BPNT1 [cytosol]", "activity_id": "176558", "ec_numbers": [ "3.1.3.7" ], "accessions": [ "O95861" ] } ], "citations": [ { "id": "PMID:10224133", "title": "Cloning and characterization of a mammalian lithium-sensitive bisphosphate 3'-nucleotidase inhibited by inositol 1,4-bisphosphate", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10224133/" } ], "x": 7740, "y": 2898.343137254902, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176609", "label": "cholesterol + PAPS =\u003e cholesterol sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:52369" ], "source_url": "https://reactome.org/content/detail/R-HSA-176609", "summary": "cholesterol + PAPS =\u003e cholesterol sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects PAPS, CHOL to PAP, cholesterol sulfate in cytosol and cross-references RHEA:52369.", "equation": "PAPS + CHOL → PAP + cholesterol sulfate", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176478", "reactome_id": "R-ALL-176478", "label": "CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176618", "reactome_id": "R-ALL-176618", "label": "cholesterol sulfate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:136579", "formula": "C27H45O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol sulfotransferase activity of SULT2B1-1 dimer [cytosol]", "activity_id": "176510", "ec_numbers": [ "2.8.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11416019", "title": "Cholesterol and hydroxycholesterol sulfotransferases: identification, distinction from dehydroepiandrosterone sulfotransferase, and differential tissue expression", "journal": "Endocrinology", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11416019/" }, { "id": "PMID:12730293", "title": "Cholesterol sulfate in human physiology: what's it all about?", "journal": "J Lipid Res", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12730293/" }, { "id": "PMID:12145317", "title": "Mutational analysis of human hydroxysteroid sulfotransferase SULT2B1 isoforms reveals that exon 1B of the SULT2B1 gene produces cholesterol sulfotransferase, whereas exon 1A yields pregnenolone sulfotransferase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12145317/" } ], "x": 8018.666666666667, "y": 2898.343137254902, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176631", "label": "dehydroepiandrosterone (DHEA) + PAPS =\u003e DHEA sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:51217" ], "source_url": "https://reactome.org/content/detail/R-HSA-176631", "summary": "dehydroepiandrosterone (DHEA) + PAPS =\u003e DHEA sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects PAPS, DHEA to PAP, DHEA-SO4 in cytosol and cross-references RHEA:51217.", "equation": "PAPS + DHEA → PAP + DHEA-SO4", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176626", "reactome_id": "R-ALL-176626", "label": "DHEA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28689", "formula": "C19H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176496", "reactome_id": "R-ALL-176496", "label": "DHEA-SO4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57905", "formula": "C19H27O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid sulfotransferase activity of SULTs active on DHEA [cytosol]", "activity_id": "176602", "ec_numbers": [ "2.8.2.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:11457664", "title": "Expression and characterization of the human 3 beta-hydroxysteroid sulfotransferases (SULT2B1a and SULT2B1b)", "journal": "J Steroid Biochem Mol Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11457664/" }, { "id": "PMID:8185618", "title": "Human liver estrogen sulfotransferase: identification by cDNA cloning and expression", "journal": "Biochem Biophys Res Commun", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8185618/" }, { "id": "PMID:2268288", "title": "Human liver steroid sulphotransferase sulphates bile acids", "journal": "Biochem J", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2268288/" } ], "x": 7461.333333333333, "y": 2905.970588235294, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176646", "label": "p-nitrophenol + PAPS =\u003e p-nitrophenol sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:66549" ], "source_url": "https://reactome.org/content/detail/R-HSA-176646", "summary": "p-nitrophenol + PAPS =\u003e p-nitrophenol sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects PAPS, PNP to PAP, p-nitrophenol sulfate in cytosol and cross-references RHEA:66549.", "equation": "PAPS + PNP → PAP + p-nitrophenol sulfate", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5695950", "reactome_id": "R-ALL-5695950", "label": "PNP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16836", "formula": "C6H5NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176600", "reactome_id": "R-ALL-176600", "label": "p-nitrophenol sulfate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35422", "formula": "C6H5NO6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aryl sulfotransferase activity of SULT1A1,A2,C2,4A1 [cytosol]", "activity_id": "176535", "ec_numbers": [ "2.8.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12039030", "title": "Highly conserved mouse and human brain sulfotransferases: molecular cloning, expression, and functional characterization", "journal": "Gene", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12039030/" }, { "id": "PMID:9852044", "title": "Molecular cloning, expression, and characterization of novel human SULT1C sulfotransferases that catalyze the sulfonation of N-hydroxy-2-acetylaminofluorene", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9852044/" }, { "id": "PMID:9566739", "title": "Structural and functional characterisation of human sulfotransferases", "journal": "Chem Biol Interact", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9566739/" }, { "id": "PMID:8697101", "title": "cDNA cloning and expression of a new form of human aryl sulfotransferase", "journal": "Int J Biochem Cell Biol", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8697101/" } ], "x": 7740, "y": 2905.970588235294, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176664", "label": "estrone + PAPS =\u003e estrone 3-sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15974" ], "source_url": "https://reactome.org/content/detail/R-HSA-176664", "summary": "estrone + PAPS =\u003e estrone 3-sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects PAPS, E1 to PAP, E1S in cytosol and cross-references RHEA:15974.", "equation": "PAPS + E1 → PAP + E1S", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176575", "reactome_id": "R-ALL-176575", "label": "E1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17263", "formula": "C18H22O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176501", "reactome_id": "R-ALL-176501", "label": "E1S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60050", "formula": "C18H21O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "estrone sulfotransferase activity of SULT1E1,2A1 [cytosol]", "activity_id": "176657", "ec_numbers": [ "2.8.2.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:7779757", "title": "Bacterial expression and characterization of a cDNA for human liver estrogen sulfotransferase", "journal": "J Steroid Biochem Mol Biol", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7779757/" }, { "id": "PMID:7678732", "title": "Cloning and expression of human liver dehydroepiandrosterone sulphotransferase", "journal": "Biochem J", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7678732/" }, { "id": "PMID:8185618", "title": "Human liver estrogen sulfotransferase: identification by cDNA cloning and expression", "journal": "Biochem Biophys Res Commun", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8185618/" } ], "x": 8018.666666666667, "y": 2905.970588235294, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-176669", "label": "N-hydroxy-2-acetylaminofluorene + PAPS =\u003e 2-acetylaminofluorene-N-sulfate + PAP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-176669", "summary": "N-hydroxy-2-acetylaminofluorene + PAPS =\u003e 2-acetylaminofluorene-N-sulfate + PAP is a Reactome release 97 curated reaction event. The source record connects N-hydroxy-2-acetylaminofluorene, PAPS to AAF-N-S, PAP in cytosol.", "equation": "N-hydroxy-2-acetylaminofluorene + PAPS → AAF-N-S + PAP", "inputs": [ { "node_id": "R-ALL-176566", "reactome_id": "R-ALL-176566", "label": "N-hydroxy-2-acetylaminofluorene", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17931", "formula": "C15H13NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-176500", "reactome_id": "R-ALL-176500", "label": "AAF-N-S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35424", "formula": "C15H13NO5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfotransferase activity of SULTs active on NH2AAF [cytosol]", "activity_id": "176520", "ec_numbers": [ "2.8.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11154739", "title": "Sulfotransferases in the bioactivation of xenobiotics", "journal": "Chem Biol Interact", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11154739/" }, { "id": "PMID:9852044", "title": "Molecular cloning, expression, and characterization of novel human SULT1C sulfotransferases that catalyze the sulfonation of N-hydroxy-2-acetylaminofluorene", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9852044/" } ], "x": 7740, "y": 3089.029411764706, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-177157", "label": "phenylacetate + Coenzyme A + ATP =\u003e phenylacetyl-CoA + AMP + pyrophosphate", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-177157", "summary": "phenylacetate + Coenzyme A + ATP =\u003e phenylacetyl-CoA + AMP + pyrophosphate is a Reactome release 97 curated reaction event. The source record connects phenylacetate, ATP, CoA-SH to phenylacetyl-CoA, AMP, PPi in mitochondrial matrix.", "equation": "phenylacetate + ATP + CoA-SH → phenylacetyl-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-177153", "reactome_id": "R-ALL-177153", "label": "phenylacetate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:8082", "formula": "C8H8O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-177137", "reactome_id": "R-ALL-177137", "label": "phenylacetyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57390", "formula": "C29H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "CoA-ligase activity of xenobiotic/medium-chain fatty acid:CoA ligases [mitochondrial matrix]", "activity_id": "177146", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10434065", "title": "Characterization of the CoA ligases of human liver mitochondria catalyzing the activation of short- and medium-chain fatty acids and xenobiotic carboxylic acids", "journal": "Biochim Biophys Acta", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10434065/" }, { "id": "PMID:12616642", "title": "Isolation, sequencing, and expression of a cDNA for the HXM-A form of xenobiotic/medium-chain fatty acid:CoA ligase from human liver mitochondria", "journal": "J Biochem Mol Toxicol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12616642/" }, { "id": "PMID:11470804", "title": "Molecular identification and characterization of two medium-chain acyl-CoA synthetases, MACS1 and the Sa gene product", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11470804/" } ], "x": 8018.666666666667, "y": 3043.264705882353, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-177160", "label": "phenylacetyl-CoA + glutamine =\u003e phenylacetyl glutamine + Coenzyme A", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:21845" ], "source_url": "https://reactome.org/content/detail/R-HSA-177160", "summary": "phenylacetyl-CoA + glutamine =\u003e phenylacetyl glutamine + Coenzyme A is a Reactome release 97 curated reaction event. The source record connects phenylacetyl-CoA, L-Gln to phenylacetyl glutamine, CoA-SH in mitochondrial matrix and cross-references RHEA:21845.", "equation": "phenylacetyl-CoA + L-Gln → phenylacetyl glutamine + CoA-SH", "inputs": [ { "node_id": "R-ALL-177137", "reactome_id": "R-ALL-177137", "label": "phenylacetyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57390", "formula": "C29H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113522", "reactome_id": "R-ALL-113522", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-177152", "reactome_id": "R-ALL-177152", "label": "phenylacetyl glutamine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58310", "formula": "C13H15N2O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-glutamine N-acyltransferase activity of glutamine-N-acyltransferase [mitochondrial matrix]", "activity_id": "177155", "ec_numbers": [ "2.3.1.68" ], "accessions": [] } ], "citations": [ { "id": "PMID:931988", "title": "Identification of separate acyl- CoA:glycine and acyl-CoA:L-glutamine N-acyltransferase activities in mitochondrial fractions from liver of rhesus monkey and man", "journal": "J Biol Chem", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/931988/" } ], "x": 7461.333333333333, "y": 2913.598039215686, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-177784", "label": "RPIA isomerizes ribose 5-phosphate to D-ribulose 5-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14658" ], "source_url": "https://reactome.org/content/detail/R-HSA-177784", "summary": "RPIA isomerizes ribose 5-phosphate to D-ribulose 5-phosphate is a Reactome release 97 curated reaction event. The source record connects R5P to RU5P in cytosol and cross-references RHEA:14658.", "equation": "R5P → RU5P", "inputs": [ { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29732", "reactome_id": "R-ALL-29732", "label": "RU5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58121", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ribose-5-phosphate isomerase activity of RPIA [cytosol]", "activity_id": "71305", "ec_numbers": [ "5.3.1.6" ], "accessions": [ "P49247" ] } ], "citations": [ { "id": "PMID:14988808", "title": "Ribose-5-phosphate isomerase deficiency: new inborn error in the pentose phosphate pathway associated with a slowly progressive leukoencephalopathy", "journal": "Am J Hum Genet", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14988808/" } ], "x": 868.6666666666667, "y": 1047.642857142857, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1793176", "label": "DS is cleaved from its proteoglycan", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1793176", "summary": "DS is cleaved from its proteoglycan is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects H2O, D2,4,4(S)3-PGs to CS/DS core proteins, ChEBI:63519 chain in lysosomal lumen.", "equation": "H2O + D2,4,4(S)3-PGs → CS/DS core proteins + ChEBI:63519 chain", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2065138", "reactome_id": "R-HSA-2065138", "label": "D2,4,4(S)3-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2065248", "reactome_id": "R-HSA-2065248", "label": "CS/DS core proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-2065231", "reactome_id": "R-ALL-2065231", "label": "ChEBI:63519 chain", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747399", "formula": "C45H68N2O46S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity, acting on glycosyl bonds of beta-xylosidase [lysosomal lumen]", "activity_id": "2065101", "ec_numbers": [ "3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:3134052", "title": "Demonstration of an endo-beta-galactosidase and an endo-beta-xylosidase that degrade the proteoglycan linkage region", "journal": "Biochim Biophys Acta", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3134052/" } ], "x": 2381.3333333333335, "y": 1066.1666666666665, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-1793182", "label": "IDS hydrolyses dermatan sulfate (Chebi:63517 chain)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1793182", "summary": "IDS hydrolyses dermatan sulfate (Chebi:63517 chain) is a Reactome release 97 curated reaction event. The source record connects H2O, ChEBI:63517 chain to SO4(2-), ChEBI:63516 chain in lysosomal lumen.", "equation": "H2O + ChEBI:63517 chain → SO4(2-) + ChEBI:63516 chain", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2065213", "reactome_id": "R-ALL-2065213", "label": "ChEBI:63517 chain", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747397", "formula": "C45H68N2O43S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2065269", "reactome_id": "R-ALL-2065269", "label": "ChEBI:63516 chain", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747395", "formula": "C45H69N2O40S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "iduronate-2-sulfatase activity of IDS dimer [lysosomal lumen]", "activity_id": "1678645", "ec_numbers": [ "3.1.6.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:2122463", "title": "Hunter syndrome: isolation of an iduronate-2-sulfatase cDNA clone and analysis of patient DNA", "journal": "Proc Natl Acad Sci U S A", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2122463/" } ], "x": 2938.666666666667, "y": 948.2878787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1793186", "label": "IDUA hydrolyses the unsulfated alpha-L-iduronosidic link in DS", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1793186", "summary": "IDUA hydrolyses the unsulfated alpha-L-iduronosidic link in DS is a Reactome release 97 curated reaction event. The source record connects H2O, IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl to aldehydo-L-iduronic acid, CHEBI:63868 chain in lysosomal lumen.", "equation": "H2O + IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl → aldehydo-L-iduronic acid + CHEBI:63868 chain", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2105025", "reactome_id": "R-ALL-2105025", "label": "IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63873", "formula": "C37H59NO35S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1678617", "reactome_id": "R-ALL-1678617", "label": "aldehydo-L-iduronic acid", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:21338", "formula": "C6H9O7", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2104993", "reactome_id": "R-ALL-2104993", "label": "CHEBI:63868 chain", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63868", "formula": "C31H51NO29S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-iduronidase activity of IDUA [lysosomal lumen]", "activity_id": "1678683", "ec_numbers": [ "3.2.1.76" ], "accessions": [ "P35475" ] } ], "citations": [ { "id": "PMID:1946389", "title": "Human alpha-L-iduronidase: cDNA isolation and expression", "journal": "Proc Natl Acad Sci U S A", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1946389/" }, { "id": "PMID:8213840", "title": "Identification of mutations in the alpha-L-iduronidase gene (IDUA) that cause Hurler and Scheie syndromes", "journal": "Am J Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8213840/" } ], "x": 2381.3333333333335, "y": 960.0757575757575, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1793207", "label": "ARSB hydrolyses C4S/C6S chains", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1793207", "summary": "ARSB hydrolyses C4S/C6S chains is a Reactome release 97 curated reaction event. The source record connects H2O, C4S/C6S chains to SO4(2-), Chondroitin chain in lysosomal lumen.", "equation": "H2O + C4S/C6S chains → SO4(2-) + Chondroitin chain", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2065251", "reactome_id": "R-ALL-2065251", "label": "C4S/C6S chains", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2065239", "reactome_id": "R-ALL-2065239", "label": "Chondroitin chain", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747387", "formula": "C45H70N2O37", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acetylgalactosamine-4-sulfatase activity of ARSB:Ca2+ [lysosomal lumen]", "activity_id": "1606816", "ec_numbers": [ "3.1.6.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:738706", "title": "Arylsulphatase B, an exo-sulphatase for chondroitin 4-sulphate tetrasaccharide", "journal": "Hoppe Seylers Z Physiol Chem", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/738706/" }, { "id": "PMID:12888274", "title": "Crystal structure of a covalent intermediate of endogenous human arylsulfatase A", "journal": "J Inorg Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12888274/" }, { "id": "PMID:9521684", "title": "Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis", "journal": "Biochemistry", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9521684/" }, { "id": "PMID:7628016", "title": "A novel amino acid modification in sulfatases that is defective in multiple sulfatase deficiency", "journal": "Cell", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7628016/" } ], "x": 2938.666666666667, "y": 983.6515151515151, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1793209", "label": "HYAL1-4 hydrolyze chondroitin chains", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1793209", "summary": "HYAL1-4 hydrolyze chondroitin chains is a Reactome release 97 curated reaction event. The source record connects H2O, Chondroitin chains to CS/HS precursor in lysosomal lumen.", "equation": "H2O + Chondroitin chains → CS/HS precursor", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2065257", "reactome_id": "R-ALL-2065257", "label": "Chondroitin chains", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2065212", "reactome_id": "R-ALL-2065212", "label": "CS/HS precursor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747385", "formula": "C23H37O21", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hyalurononglucosaminidase activity of HYAL1-like proteins [lysosomal lumen]", "activity_id": "1793220", "ec_numbers": [ "3.2.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:9223416", "title": "Purification, cloning, and expression of human plasma hyaluronidase", "journal": "Biochem Biophys Res Commun", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9223416/" }, { "id": "PMID:19889881", "title": "Identification of human hyaluronidase-4 as a novel chondroitin sulfate hydrolase that preferentially cleaves the galactosaminidic linkage in the trisulfated tetrasaccharide sequence", "journal": "Glycobiology", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19889881/" }, { "id": "PMID:10339581", "title": "Mutations in HYAL1, a member of a tandemly distributed multigene family encoding disparate hyaluronidase activities, cause a newly described lysosomal disorder, mucopolysaccharidosis IX", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10339581/" } ], "x": 2381.3333333333335, "y": 1030.8030303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1793217", "label": "Unknown endo-β-galactosidase cleaves keratan chain", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1793217", "summary": "Unknown endo-β-galactosidase cleaves keratan chain is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects H2O, KSPG(2) to KS core proteins, Gal(S)-GlcNAc(S)-Gal-GlcNAc(S)-Gal in lysosomal lumen.", "equation": "H2O + KSPG(2) → KS core proteins + Gal(S)-GlcNAc(S)-Gal-GlcNAc(S)-Gal", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2046191", "reactome_id": "R-HSA-2046191", "label": "KSPG(2)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2105011", "reactome_id": "R-HSA-2105011", "label": "KS core proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-2105007", "reactome_id": "R-ALL-2105007", "label": "Gal(S)-GlcNAc(S)-Gal-GlcNAc(S)-Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63846", "formula": "C34H58N2O35S3", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-galactosidase activity of keratan sulfate 1,4-beta-D-galactosidase [lysosomal lumen]", "activity_id": "1793215", "ec_numbers": [ "3.2.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:5822067", "title": "Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase", "journal": "Biochem J", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5822067/" } ], "x": 2660, "y": 1066.1666666666665, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-1855153", "label": "I(1,4,5)P3 is phosphorylated to I(1,3,4,5)P4 by ITPKA/B/C in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11021" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855153", "summary": "I(1,4,5)P3 is phosphorylated to I(1,3,4,5)P4 by ITPKA/B/C in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,4,5)P3, ATP to I(1,3,4,5)P4, ADP, H+ in cytosol and cross-references RHEA:11021.", "equation": "I(1,4,5)P3 + ATP → I(1,3,4,5)P4 + ADP + H+", "inputs": [ { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994170", "reactome_id": "R-ALL-994170", "label": "I(1,3,4,5)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,4,5-trisphosphate 3-kinase activity of active ITPKA/B/C [cytosol]", "activity_id": "2023862", "ec_numbers": [ "2.7.1.127" ], "accessions": [] } ], "citations": [ { "id": "PMID:12747803", "title": "The three isoenzymes of human inositol-1,4,5-trisphosphate 3-kinase show specific intracellular localization but comparable Ca2+ responses on transfection in COS-7 cells", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12747803/" } ], "x": 7461.333333333333, "y": 750.1041666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855154", "label": "I1P is dephosphorylated to Ins by IMPA1/2 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27671" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855154", "summary": "I1P is dephosphorylated to Ins by IMPA1/2 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I1P, H2O to Ins, Pi in cytosol and cross-references RHEA:27671.", "equation": "I1P + H2O → Ins + Pi", "inputs": [ { "node_id": "R-ALL-870330", "reactome_id": "R-ALL-870330", "label": "I1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58433", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426910", "reactome_id": "R-ALL-426910", "label": "Ins", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17268", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol monophosphate 1-phosphatase activity of IMPA1/2 [cytosol]", "activity_id": "2024043", "ec_numbers": [ "3.1.3.25" ], "accessions": [] } ], "citations": [ { "id": "PMID:1377913", "title": "cDNA cloning of human and rat brain myo-inositol monophosphatase. Expression and characterization of the human recombinant enzyme", "journal": "Biochem J", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1377913/" }, { "id": "PMID:17068342", "title": "Spatial expression patterns and biochemical properties distinguish a second myo-inositol monophosphatase IMPA2 from IMPA1", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17068342/" } ], "x": 7461.333333333333, "y": 814.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855155", "label": "I(1,3,4,5,6)P5 transports from the ER lumen to the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "endoplasmic reticulum lumen · cytosol", "compartment_ids": [ "GO:0005788", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855155", "summary": "I(1,3,4,5,6)P5 transports from the ER lumen to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects I(1,3,4,5,6)P5 to I(1,3,4,5,6)P5 in endoplasmic reticulum lumen · cytosol.", "equation": "I(1,3,4,5,6)P5 → I(1,3,4,5,6)P5", "inputs": [ { "node_id": "R-ALL-2024008", "reactome_id": "R-ALL-2024008", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-994141", "reactome_id": "R-ALL-994141", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "myo-inositol transmembrane transporter activity of unknown PI transporter [endoplasmic reticulum membrane]", "activity_id": "9954154", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" }, { "id": "PMID:11909533", "title": "Regulation of Ins(3,4,5,6)P(4) signaling by a reversible kinase/phosphatase", "journal": "Curr Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11909533/" }, { "id": "PMID:17705785", "title": "Intracellular localization of human Ins(1,3,4,5,6)P5 2-kinase", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17705785/" } ], "x": 7740, "y": 1025.6458333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855157", "label": "1-PP-IP5 is phosphorylated to 1,5-(PP)2-IP4 by IP6K1/2 in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:37468" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855157", "summary": "1-PP-IP5 is phosphorylated to 1,5-(PP)2-IP4 by IP6K1/2 in the nucleus is a Reactome release 97 curated reaction event. The source record connects 1-PP-IP5, ATP, H+ to 1,5-(PP)2-IP4, ADP in nucleoplasm and cross-references RHEA:37468.", "equation": "1-PP-IP5 + ATP + H+ → 1,5-(PP)2-IP4 + ADP", "inputs": [ { "node_id": "R-ALL-2023932", "reactome_id": "R-ALL-2023932", "label": "1-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:74946", "formula": "C6H6O27P7", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023931", "reactome_id": "R-ALL-2023931", "label": "1,5-(PP)2-IP4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77983", "formula": "C6H7O30P8", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diphosphoinositol pentakisphosphate kinase activity of IP6K1/2 [nucleoplasm]", "activity_id": "2023919", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:17412958", "title": "A conserved family of enzymes that phosphorylate inositol hexakisphosphate", "journal": "Science", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17412958/" } ], "x": 7740, "y": 750.1041666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855158", "label": "5-PP-IP5 is phosphorylated to 5-PPP-IP5 by IP6K1/3 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855158", "summary": "5-PP-IP5 is phosphorylated to 5-PPP-IP5 by IP6K1/3 in the cytosol is a Reactome release 97 curated reaction event. The source record connects 5-PP-IP5, ATP to 5-PPP-IP5, ADP in cytosol.", "equation": "5-PP-IP5 + ATP → 5-PPP-IP5 + ADP", "inputs": [ { "node_id": "R-ALL-1629765", "reactome_id": "R-ALL-1629765", "label": "5-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629759", "reactome_id": "R-ALL-1629759", "label": "5-PPP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62985", "formula": "C6H20O30P8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol 5-diphosphate pentakisphosphate 5-kinase activity of IP6K1/3 [cytosol]", "activity_id": "2023988", "ec_numbers": [ "2.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:18355727", "title": "Inositol hexakisphosphate kinase products contain diphosphate and triphosphate groups", "journal": "Chem Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18355727/" } ], "x": 7461.333333333333, "y": 960.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855159", "label": "I(1,3,4,6)P4 is dephosphorylated to I(3,4,6)P3 by ITPK1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855159", "summary": "I(1,3,4,6)P4 is dephosphorylated to I(3,4,6)P3 by ITPK1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,6)P4, H2O to I(3,4,6)P3, Pi in cytosol.", "equation": "I(1,3,4,6)P4 + H2O → I(3,4,6)P3 + Pi", "inputs": [ { "node_id": "R-ALL-994146", "reactome_id": "R-ALL-994146", "label": "I(1,3,4,6)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57660", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629741", "reactome_id": "R-ALL-1629741", "label": "I(3,4,6)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:189099", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4,6-tetrakisphosphate 1-phosphatase activity of ITPK1:Mg2+ [cytosol]", "activity_id": "2023909", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:11909533", "title": "Regulation of Ins(3,4,5,6)P(4) signaling by a reversible kinase/phosphatase", "journal": "Curr Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11909533/" } ], "x": 7740, "y": 960.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855160", "label": "I(1,3,4,5,6)P5 transports from the ER lumen to the nucleus", "schema_class": "BlackBoxEvent", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "endoplasmic reticulum lumen · nucleoplasm", "compartment_ids": [ "GO:0005788", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855160", "summary": "I(1,3,4,5,6)P5 transports from the ER lumen to the nucleus is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects I(1,3,4,5,6)P5 to I(1,3,4,5,6)P5 in endoplasmic reticulum lumen · nucleoplasm.", "equation": "I(1,3,4,5,6)P5 → I(1,3,4,5,6)P5", "inputs": [ { "node_id": "R-ALL-2024008", "reactome_id": "R-ALL-2024008", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2023901", "reactome_id": "R-ALL-2023901", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12084730", "title": "The synthesis of inositol hexakisphosphate. Characterization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12084730/" }, { "id": "PMID:17705785", "title": "Intracellular localization of human Ins(1,3,4,5,6)P5 2-kinase", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17705785/" }, { "id": "PMID:17702752", "title": "Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17702752/" } ], "x": 8018.666666666667, "y": 1106.6875, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1855161", "label": "I(1,3,4,5,6)P5 transports from the nucleus to the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855161", "summary": "I(1,3,4,5,6)P5 transports from the nucleus to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects I(1,3,4,5,6)P5 to I(1,3,4,5,6)P5 in nucleoplasm · cytosol.", "equation": "I(1,3,4,5,6)P5 → I(1,3,4,5,6)P5", "inputs": [ { "node_id": "R-ALL-2023901", "reactome_id": "R-ALL-2023901", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-994141", "reactome_id": "R-ALL-994141", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" }, { "id": "PMID:11909533", "title": "Regulation of Ins(3,4,5,6)P(4) signaling by a reversible kinase/phosphatase", "journal": "Curr Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11909533/" }, { "id": "PMID:17705785", "title": "Intracellular localization of human Ins(1,3,4,5,6)P5 2-kinase", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17705785/" } ], "x": 8018.666666666667, "y": 1025.6458333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855162", "label": "I(3,4,5,6)P4 is phosphorylated to I(1,3,4,5,6)P5 by ITPK1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12453" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855162", "summary": "I(3,4,5,6)P4 is phosphorylated to I(1,3,4,5,6)P5 by ITPK1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(3,4,5,6)P4, ATP to I(1,3,4,5,6)P5, ADP in cytosol and cross-references RHEA:12453.", "equation": "I(3,4,5,6)P4 + ATP → I(1,3,4,5,6)P5 + ADP", "inputs": [ { "node_id": "R-ALL-994149", "reactome_id": "R-ALL-994149", "label": "I(3,4,5,6)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57539", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994141", "reactome_id": "R-ALL-994141", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-3,4,5,6-tetrakisphosphate 1-kinase activity of ITPK1:Mg2+ [cytosol]", "activity_id": "2023985", "ec_numbers": [ "2.7.1.134" ], "accessions": [] } ], "citations": [ { "id": "PMID:11042108", "title": "Multitasking in signal transduction by a promiscuous human Ins(3,4,5,6)P(4) 1-kinase/Ins(1,3,4)P(3) 5/6-kinase", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11042108/" } ], "x": 8018.666666666667, "y": 912.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855163", "label": "I(1,3,4,5,6)P5 is dephosphorylated to I(1,4,5,6)P4 by MINPP1 in the ER lumen", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [ "RHEA:77144" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855163", "summary": "I(1,3,4,5,6)P5 is dephosphorylated to I(1,4,5,6)P4 by MINPP1 in the ER lumen is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5,6)P5, H2O to I(1,4,5,6)P4, Pi in endoplasmic reticulum lumen and cross-references RHEA:77144.", "equation": "I(1,3,4,5,6)P5 + H2O → I(1,4,5,6)P4 + Pi", "inputs": [ { "node_id": "R-ALL-2024008", "reactome_id": "R-ALL-2024008", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2024012", "reactome_id": "R-ALL-2024012", "label": "I(1,4,5,6)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57627", "formula": "C6H8O18P4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113551", "reactome_id": "R-ALL-113551", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4,5,6-pentakisphosphate 3-phosphatase activity of MINPP1 [endoplasmic reticulum lumen]", "activity_id": "2024013", "ec_numbers": [ "3.1.3" ], "accessions": [ "Q9UNW1" ] } ], "citations": [ { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" }, { "id": "PMID:10087200", "title": "Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10087200/" } ], "x": 7740, "y": 814.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855164", "label": "IP6 transports from the cytosol to the ER lumen", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005829", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855164", "summary": "IP6 transports from the cytosol to the ER lumen is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects IP6 to IP6 in cytosol · endoplasmic reticulum lumen.", "equation": "IP6 → IP6", "inputs": [ { "node_id": "R-ALL-1629770", "reactome_id": "R-ALL-1629770", "label": "IP6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2024018", "reactome_id": "R-ALL-2024018", "label": "IP6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "myo-inositol transmembrane transporter activity of unknown PI transporter [endoplasmic reticulum membrane]", "activity_id": "9954154", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" } ], "x": 7461.333333333333, "y": 1041.8541666666665, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855165", "label": "(PP)2-IP4 is dephosphorylated to 5-PP-IP5 by NUDT(1) in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855165", "summary": "(PP)2-IP4 is dephosphorylated to 5-PP-IP5 by NUDT(1) in the cytosol is a Reactome release 97 curated reaction event. The source record connects (PP)2-IP4, H2O to 5-PP-IP5, Pi in cytosol.", "equation": "(PP)2-IP4 + H2O → 5-PP-IP5 + Pi", "inputs": [ { "node_id": "R-ALL-2023968", "reactome_id": "R-ALL-2023968", "label": "(PP)2-IP4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629765", "reactome_id": "R-ALL-1629765", "label": "5-PP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol bisdiphosphate tetrakisphosphate diphosphatase activity of NUDT(1) [cytosol]", "activity_id": "2023955", "ec_numbers": [ "3.6.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:12105228", "title": "An adjacent pair of human NUDT genes on chromosome X are preferentially expressed in testis and encode two new isoforms of diphosphoinositol polyphosphate phosphohydrolase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12105228/" }, { "id": "PMID:10419486", "title": "The diadenosine hexaphosphate hydrolases from Schizosaccharomyces pombe and Saccharomyces cerevisiae are homologues of the human diphosphoinositol polyphosphate phosphohydrolase. Overlapping substrate specificities in a MutT-type protein", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419486/" }, { "id": "PMID:9822604", "title": "A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase", "journal": "EMBO J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9822604/" }, { "id": "PMID:10585413", "title": "Site-directed mutagenesis of diphosphoinositol polyphosphate phosphohydrolase, a dual specificity NUDT enzyme that attacks diadenosine polyphosphates and diphosphoinositol polyphosphates", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10585413/" }, { "id": "PMID:10777568", "title": "Discovery of molecular and catalytic diversity among human diphosphoinositol-polyphosphate phosphohydrolases. An expanding Nudt family", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777568/" }, { "id": "PMID:12121577", "title": "Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases", "journal": "BMC Biochem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12121577/" } ], "x": 7740, "y": 1009.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855166", "label": "PP-IP4 is dephosphorylated to I(1,3,4,5,6)P5 by NUDT4 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855166", "summary": "PP-IP4 is dephosphorylated to I(1,3,4,5,6)P5 by NUDT4 in the cytosol is a Reactome release 97 curated reaction event. The source record connects PP-IP4, H2O to I(1,3,4,5,6)P5, Pi in cytosol.", "equation": "PP-IP4 + H2O → I(1,3,4,5,6)P5 + Pi", "inputs": [ { "node_id": "R-ALL-2023943", "reactome_id": "R-ALL-2023943", "label": "PP-IP4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994141", "reactome_id": "R-ALL-994141", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol diphosphate tetrakisphosphate diphosphatase activity of NUDT4:Mg2+/Mn2+ [cytosol]", "activity_id": "2023970", "ec_numbers": [ "3.6.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:10827188", "title": "The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10827188/" } ], "x": 8018.666666666667, "y": 1009.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855168", "label": "I(1,3,4,5)P4 transports from the cytosol to the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855168", "summary": "I(1,3,4,5)P4 transports from the cytosol to the nucleus is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects I(1,3,4,5)P4 to I(1,3,4,5)P4 in cytosol · nucleoplasm.", "equation": "I(1,3,4,5)P4 → I(1,3,4,5)P4", "inputs": [ { "node_id": "R-ALL-994170", "reactome_id": "R-ALL-994170", "label": "I(1,3,4,5)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2023891", "reactome_id": "R-ALL-2023891", "label": "I(1,3,4,5)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12747803", "title": "The three isoenzymes of human inositol-1,4,5-trisphosphate 3-kinase show specific intracellular localization but comparable Ca2+ responses on transfection in COS-7 cells", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12747803/" }, { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" } ], "x": 7740, "y": 1041.8541666666665, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855169", "label": "I(3,4,6)P3 is phosphorylated to I(1,3,4,6)P4 by ITPK1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:70288" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855169", "summary": "I(3,4,6)P3 is phosphorylated to I(1,3,4,6)P4 by ITPK1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(3,4,6)P3, ATP to I(1,3,4,6)P4, ADP, H+ in cytosol and cross-references RHEA:70288.", "equation": "I(3,4,6)P3 + ATP → I(1,3,4,6)P4 + ADP + H+", "inputs": [ { "node_id": "R-ALL-1629741", "reactome_id": "R-ALL-1629741", "label": "I(3,4,6)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:189099", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994146", "reactome_id": "R-ALL-994146", "label": "I(1,3,4,6)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57660", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-3,4,6-trisphosphate 1-kinase activity of ITPK1:Mg2+ [cytosol]", "activity_id": "2023895", "ec_numbers": [ "2.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11909533", "title": "Regulation of Ins(3,4,5,6)P(4) signaling by a reversible kinase/phosphatase", "journal": "Curr Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11909533/" } ], "x": 8018.666666666667, "y": 750.1041666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855171", "label": "I(1,3,4,6)P4 is dephosphorylated to I(1,3,4)P3 by ITPK1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855171", "summary": "I(1,3,4,6)P4 is dephosphorylated to I(1,3,4)P3 by ITPK1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,6)P4, H2O to I(1,3,4)P3, Pi in cytosol.", "equation": "I(1,3,4,6)P4 + H2O → I(1,3,4)P3 + Pi", "inputs": [ { "node_id": "R-ALL-994146", "reactome_id": "R-ALL-994146", "label": "I(1,3,4,6)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57660", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994123", "reactome_id": "R-ALL-994123", "label": "I(1,3,4)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58414", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4,6-tetrakisphosphate 6-phosphatase activity of ITPK1:Mg2+ [cytosol]", "activity_id": "2023900", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:11909533", "title": "Regulation of Ins(3,4,5,6)P(4) signaling by a reversible kinase/phosphatase", "journal": "Curr Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11909533/" } ], "x": 8018.666666666667, "y": 960.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855172", "label": "I(1,3,4)P3 is phosphorylated to I(1,3,4,5)P4 by ITPK1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13254" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855172", "summary": "I(1,3,4)P3 is phosphorylated to I(1,3,4,5)P4 by ITPK1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4)P3, ATP to I(1,3,4,5)P4, ADP, H+ in cytosol and cross-references RHEA:13254.", "equation": "I(1,3,4)P3 + ATP → I(1,3,4,5)P4 + ADP + H+", "inputs": [ { "node_id": "R-ALL-994123", "reactome_id": "R-ALL-994123", "label": "I(1,3,4)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58414", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994170", "reactome_id": "R-ALL-994170", "label": "I(1,3,4,5)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,3,4-trisphosphate 5-kinase activity of ITPK1:Mg2+ [cytosol]", "activity_id": "2023874", "ec_numbers": [ "2.7.1.159" ], "accessions": [] } ], "citations": [ { "id": "PMID:8662638", "title": "Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning and expression of the recombinant enzyme", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662638/" } ], "x": 7461.333333333333, "y": 766.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855173", "label": "5-PP-IP4 transports from the nucleus to the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855173", "summary": "5-PP-IP4 transports from the nucleus to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 5-PP-IP4 to 5-PP-IP4 in nucleoplasm · cytosol.", "equation": "5-PP-IP4 → 5-PP-IP4", "inputs": [ { "node_id": "R-ALL-2023922", "reactome_id": "R-ALL-2023922", "label": "5-PP-IP4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:142939", "formula": "C6H7O24P6", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1629755", "reactome_id": "R-ALL-1629755", "label": "5-PP-IP4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62928", "formula": "C6H18O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:10827188", "title": "The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10827188/" } ], "x": 8018.666666666667, "y": 1041.8541666666665, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855174", "label": "I(1,4,5)P3 is dephosphorylated to I(1,4)P2 by INPP5(4) in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19798" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855174", "summary": "I(1,4,5)P3 is dephosphorylated to I(1,4)P2 by INPP5(4) in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,4,5)P3, H2O to I(1,4)P2, Pi in cytosol and cross-references RHEA:19798.", "equation": "I(1,4,5)P3 + H2O → I(1,4)P2 + Pi", "inputs": [ { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629739", "reactome_id": "R-ALL-1629739", "label": "I(1,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58282", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,4,5-trisphosphate 5-phosphatase activity of INPP5(4) [cytosol]", "activity_id": "2024033", "ec_numbers": [ "3.1.3.56" ], "accessions": [] } ], "citations": [ { "id": "PMID:15474001", "title": "Type II phosphoinositide 5-phosphatases have unique sensitivities towards fatty acid composition and head group phosphorylation", "journal": "FEBS Lett", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15474001/" }, { "id": "PMID:10593988", "title": "Novel inositol polyphosphate 5-phosphatase localizes at membrane ruffles", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10593988/" }, { "id": "PMID:7761412", "title": "The protein deficient in Lowe syndrome is a phosphatidylinositol-4,5-bisphosphate 5-phosphatase", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7761412/" }, { "id": "PMID:9430698", "title": "Cell lines from kidney proximal tubules of a patient with Lowe syndrome lack OCRL inositol polyphosphate 5-phosphatase and accumulate phosphatidylinositol 4,5-bisphosphate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9430698/" } ], "x": 8018.666666666667, "y": 814.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855176", "label": "I(1,3,4,5,6)P5 is phosphorylated to IP6 by IPPK (IP5-2K) in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:20314" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855176", "summary": "I(1,3,4,5,6)P5 is phosphorylated to IP6 by IPPK (IP5-2K) in the nucleus is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5,6)P5, ATP to IP6, ADP, H+ in nucleoplasm and cross-references RHEA:20314.", "equation": "I(1,3,4,5,6)P5 + ATP → IP6 + ADP + H+", "inputs": [ { "node_id": "R-ALL-2023901", "reactome_id": "R-ALL-2023901", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023929", "reactome_id": "R-ALL-2023929", "label": "IP6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,3,4,5,6-pentakisphosphate 2-kinase activity of IPPK [nucleoplasm]", "activity_id": "2023933", "ec_numbers": [ "2.7.1.158" ], "accessions": [ "Q9H8X2" ] } ], "citations": [ { "id": "PMID:12084730", "title": "The synthesis of inositol hexakisphosphate. Characterization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12084730/" }, { "id": "PMID:17705785", "title": "Intracellular localization of human Ins(1,3,4,5,6)P5 2-kinase", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17705785/" }, { "id": "PMID:17702752", "title": "Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17702752/" } ], "x": 7740, "y": 766.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855177", "label": "PI(4,5)P2 is hydrolysed to I(1,4,5)P3 and DAG by cytosolic PLC[2] at the plasma membrane", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [ "RHEA:33180", "RHEA:33179" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855177", "summary": "PI(4,5)P2 is hydrolysed to I(1,4,5)P3 and DAG by cytosolic PLC[2] at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(4,5)P2, H2O to DAG, I(1,4,5)P3, H+ in cytosol · plasma membrane and cross-references RHEA:33180, RHEA:33179.", "equation": "PI(4,5)P2 + H2O → DAG + I(1,4,5)P3 + H+", "inputs": [ { "node_id": "R-ALL-179856", "reactome_id": "R-ALL-179856", "label": "PI(4,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-114519", "reactome_id": "R-ALL-114519", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphatidylinositol-4,5-bisphosphate phospholipase C activity of PLCbz [cytosol]", "activity_id": "2023847", "ec_numbers": [ "3.1.4.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:11118617", "title": "Cloning and characterization of the human phosphoinositide-specific phospholipase C-beta 1 (PLC beta 1)", "journal": "Biochim Biophys Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11118617/" }, { "id": "PMID:1333955", "title": "Identification, purification and characterization of a novel phosphatidylinositol-specific phospholipase C, a third member of the beta subfamily", "journal": "Eur J Biochem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1333955/" }, { "id": "PMID:15790568", "title": "The role of EF-hand domains and C2 domain in regulation of enzymatic activity of phospholipase Czeta", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15790568/" }, { "id": "PMID:8454637", "title": "Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8454637/" }, { "id": "PMID:8530101", "title": "cDNA sequence and gene locus of the human retinal phosphoinositide-specific phospholipase-C beta 4 (PLCB4)", "journal": "Genomics", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8530101/" }, { "id": "PMID:15579586", "title": "Phospholipase Czeta causes Ca2+ oscillations and parthenogenetic activation of human oocytes", "journal": "Reproduction", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15579586/" } ], "x": 8018.666666666667, "y": 766.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855178", "label": "Glc6P is isomerised to I3P by ISYNA1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10717" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855178", "summary": "Glc6P is isomerised to I3P by ISYNA1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects Glc6P to I3P in cytosol and cross-references RHEA:10717.", "equation": "Glc6P → I3P", "inputs": [ { "node_id": "R-ALL-1629756", "reactome_id": "R-ALL-1629756", "label": "Glc6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61548", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1629731", "reactome_id": "R-ALL-1629731", "label": "I3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58401", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-3-phosphate synthase activity of ISYNA1:NAD+ [cytosol]", "activity_id": "2024047", "ec_numbers": [ "5.5.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:15024000", "title": "Human 1-D-myo-inositol-3-phosphate synthase is functional in yeast", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15024000/" } ], "x": 7461.333333333333, "y": 912.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855179", "label": "I(1,3,4,5,6)P5 is phosphorylated to IP6 by IPPK in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20314" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855179", "summary": "I(1,3,4,5,6)P5 is phosphorylated to IP6 by IPPK in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5,6)P5, ATP to IP6, ADP in cytosol and cross-references RHEA:20314.", "equation": "I(1,3,4,5,6)P5 + ATP → IP6 + ADP", "inputs": [ { "node_id": "R-ALL-994141", "reactome_id": "R-ALL-994141", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629770", "reactome_id": "R-ALL-1629770", "label": "IP6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,3,4,5,6-pentakisphosphate 2-kinase activity of IPPK [cytosol]", "activity_id": "2023961", "ec_numbers": [ "2.7.1.158" ], "accessions": [ "Q9H8X2" ] } ], "citations": [ { "id": "PMID:12084730", "title": "The synthesis of inositol hexakisphosphate. Characterization of human inositol 1,3,4,5,6-pentakisphosphate 2-kinase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12084730/" }, { "id": "PMID:17705785", "title": "Intracellular localization of human Ins(1,3,4,5,6)P5 2-kinase", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17705785/" }, { "id": "PMID:17702752", "title": "Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17702752/" } ], "x": 7461.333333333333, "y": 928.3958333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855180", "label": "I(1,3,4)P3 is dephosphorylated to I(1,3)P2 by INPP4A/B in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:43393" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855180", "summary": "I(1,3,4)P3 is dephosphorylated to I(1,3)P2 by INPP4A/B in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4)P3, H2O to I(1,3)P2, Pi in cytosol and cross-references RHEA:43393.", "equation": "I(1,3,4)P3 + H2O → I(1,3)P2 + Pi", "inputs": [ { "node_id": "R-ALL-994123", "reactome_id": "R-ALL-994123", "label": "I(1,3,4)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58414", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629769", "reactome_id": "R-ALL-1629769", "label": "I(1,3)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83242", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4-trisphosphate 4-phosphatase activity of INPP4A/B [cytosol]", "activity_id": "2024025", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:7608176", "title": "The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphatase", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7608176/" }, { "id": "PMID:9295334", "title": "The cDNA cloning and characterization of inositol polyphosphate 4-phosphatase type II. Evidence for conserved alternative splicing in the 4-phosphatase family", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9295334/" } ], "x": 7461.333333333333, "y": 831.1458333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855181", "label": "I(1,3,4,5,6)P5 is phosphorylated to 5-PP-IP4 by IP6K1/2 in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855181", "summary": "I(1,3,4,5,6)P5 is phosphorylated to 5-PP-IP4 by IP6K1/2 in the nucleus is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5,6)P5, ATP to 5-PP-IP4, ADP in nucleoplasm.", "equation": "I(1,3,4,5,6)P5 + ATP → 5-PP-IP4 + ADP", "inputs": [ { "node_id": "R-ALL-2023901", "reactome_id": "R-ALL-2023901", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023922", "reactome_id": "R-ALL-2023922", "label": "5-PP-IP4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:142939", "formula": "C6H7O24P6", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,3,4,5,6-pentakisphosphate kinase activity of IP6K1/2 [nucleoplasm]", "activity_id": "2023953", "ec_numbers": [ "2.7.4.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:10827188", "title": "The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10827188/" }, { "id": "PMID:18355727", "title": "Inositol hexakisphosphate kinase products contain diphosphate and triphosphate groups", "journal": "Chem Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18355727/" } ], "x": 7461.333333333333, "y": 977.0208333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855182", "label": "5-PP-IP5 is phosphorylated to 1,5-(PP)2-IP4 by PPIP5K1/2 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10277" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855182", "summary": "5-PP-IP5 is phosphorylated to 1,5-(PP)2-IP4 by PPIP5K1/2 in the cytosol is a Reactome release 97 curated reaction event. The source record connects 5-PP-IP5, ATP to 1,5-(PP)2-IP4, ADP in cytosol and cross-references RHEA:10277.", "equation": "5-PP-IP5 + ATP → 1,5-(PP)2-IP4 + ADP", "inputs": [ { "node_id": "R-ALL-1629765", "reactome_id": "R-ALL-1629765", "label": "5-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629732", "reactome_id": "R-ALL-1629732", "label": "1,5-(PP)2-IP4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77983", "formula": "C6H7O30P8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diphosphoinositol pentakisphosphate kinase activity of PPIP5K1/2 [cytosol]", "activity_id": "2023999", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18981179", "title": "Structural analysis and detection of biological inositol pyrophosphates reveal that the family of VIP/diphosphoinositol pentakisphosphate kinases are 1/3-kinases", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18981179/" }, { "id": "PMID:17690096", "title": "Cloning and characterization of two human VIP1-like inositol hexakisphosphate and diphosphoinositol pentakisphosphate kinases", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17690096/" }, { "id": "PMID:17412958", "title": "A conserved family of enzymes that phosphorylate inositol hexakisphosphate", "journal": "Science", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17412958/" }, { "id": "PMID:17702752", "title": "Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17702752/" } ], "x": 7740, "y": 928.3958333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855185", "label": "I(1,4,5,6)P4 is phosphorylated to I(1,3,4,5,6)P5 by IPMK in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:11857" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855185", "summary": "I(1,4,5,6)P4 is phosphorylated to I(1,3,4,5,6)P5 by IPMK in the nucleus is a Reactome release 97 curated reaction event. The source record connects I(1,4,5,6)P4, ATP to I(1,3,4,5,6)P5, ADP, H+ in nucleoplasm and cross-references RHEA:11857.", "equation": "I(1,4,5,6)P4 + ATP → I(1,3,4,5,6)P5 + ADP + H+", "inputs": [ { "node_id": "R-ALL-2023935", "reactome_id": "R-ALL-2023935", "label": "I(1,4,5,6)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57627", "formula": "C6H8O18P4", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023901", "reactome_id": "R-ALL-2023901", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,4,5,6-tetrakisphosphate 3-kinase activity of IPMK [nucleoplasm]", "activity_id": "2023945", "ec_numbers": [ "2.7.1.151" ], "accessions": [ "Q8NFU5" ] } ], "citations": [ { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" }, { "id": "PMID:16293229", "title": "Inositol polyphosphate multikinase regulates inositol 1,4,5,6-tetrakisphosphate", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16293229/" } ], "x": 7461.333333333333, "y": 782.5208333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855186", "label": "I(1,3,4,5)P4 transports from the cytosol to the ER lumen", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005829", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855186", "summary": "I(1,3,4,5)P4 transports from the cytosol to the ER lumen is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects I(1,3,4,5)P4 to I(1,3,4,5)P4 in cytosol · endoplasmic reticulum lumen.", "equation": "I(1,3,4,5)P4 → I(1,3,4,5)P4", "inputs": [ { "node_id": "R-ALL-994170", "reactome_id": "R-ALL-994170", "label": "I(1,3,4,5)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2024002", "reactome_id": "R-ALL-2024002", "label": "I(1,3,4,5)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "myo-inositol transmembrane transporter activity of unknown PI transporter [endoplasmic reticulum membrane]", "activity_id": "9954154", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" }, { "id": "PMID:10087200", "title": "Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10087200/" } ], "x": 7461.333333333333, "y": 1058.0625, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855187", "label": "IP6 transports from the nucleus to the ER lumen", "schema_class": "BlackBoxEvent", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005654", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855187", "summary": "IP6 transports from the nucleus to the ER lumen is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects IP6 to IP6 in nucleoplasm · endoplasmic reticulum lumen.", "equation": "IP6 → IP6", "inputs": [ { "node_id": "R-ALL-2023929", "reactome_id": "R-ALL-2023929", "label": "IP6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2024018", "reactome_id": "R-ALL-2024018", "label": "IP6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" } ], "x": 7461.333333333333, "y": 1122.8958333333333, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1855188", "label": "IP6 transports from the cytosol to the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855188", "summary": "IP6 transports from the cytosol to the nucleus is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects IP6 to IP6 in cytosol · nucleoplasm.", "equation": "IP6 → IP6", "inputs": [ { "node_id": "R-ALL-1629770", "reactome_id": "R-ALL-1629770", "label": "IP6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2023929", "reactome_id": "R-ALL-2023929", "label": "IP6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" } ], "x": 7740, "y": 1058.0625, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855189", "label": "I(1,4,5,6)P4 transports from the ER lumen to the nucleus", "schema_class": "BlackBoxEvent", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "endoplasmic reticulum lumen · nucleoplasm", "compartment_ids": [ "GO:0005788", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855189", "summary": "I(1,4,5,6)P4 transports from the ER lumen to the nucleus is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects I(1,4,5,6)P4 to I(1,4,5,6)P4 in endoplasmic reticulum lumen · nucleoplasm.", "equation": "I(1,4,5,6)P4 → I(1,4,5,6)P4", "inputs": [ { "node_id": "R-ALL-2024012", "reactome_id": "R-ALL-2024012", "label": "I(1,4,5,6)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57627", "formula": "C6H8O18P4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2023935", "reactome_id": "R-ALL-2023935", "label": "I(1,4,5,6)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57627", "formula": "C6H8O18P4", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" }, { "id": "PMID:10087200", "title": "Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10087200/" }, { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" } ], "x": 7740, "y": 1122.8958333333333, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-1855190", "label": "I(1,4,5)P3 transports from the cytosol to the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855190", "summary": "I(1,4,5)P3 transports from the cytosol to the nucleus is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects I(1,4,5)P3 to I(1,4,5)P3 in cytosol · nucleoplasm.", "equation": "I(1,4,5)P3 → I(1,4,5)P3", "inputs": [ { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2023907", "reactome_id": "R-ALL-2023907", "label": "I(1,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12747803", "title": "The three isoenzymes of human inositol-1,4,5-trisphosphate 3-kinase show specific intracellular localization but comparable Ca2+ responses on transfection in COS-7 cells", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12747803/" }, { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" } ], "x": 8018.666666666667, "y": 1058.0625, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855193", "label": "1-PP-IP4 is phosphorylated to 1,5-(PP)2-IP3 by IP6K1/3 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855193", "summary": "1-PP-IP4 is phosphorylated to 1,5-(PP)2-IP3 by IP6K1/3 in the cytosol is a Reactome release 97 curated reaction event. The source record connects 1-PP-IP4, ATP to 1,5-(PP)2-IP3, ADP in cytosol.", "equation": "1-PP-IP4 + ATP → 1,5-(PP)2-IP3 + ADP", "inputs": [ { "node_id": "R-ALL-1629736", "reactome_id": "R-ALL-1629736", "label": "1-PP-IP4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62926", "formula": "C6H18O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629738", "reactome_id": "R-ALL-1629738", "label": "1,5-(PP)2-IP3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62931", "formula": "C6H19O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diphosphoinositol tetrakisphosphate kinase activity of IP6K1/3 [cytosol]", "activity_id": "2023982", "ec_numbers": [ "2.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:10827188", "title": "The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10827188/" }, { "id": "PMID:18355727", "title": "Inositol hexakisphosphate kinase products contain diphosphate and triphosphate groups", "journal": "Chem Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18355727/" } ], "x": 7740, "y": 977.0208333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855194", "label": "1-PP-IP5 is phosphorylated to 1,5-(PP)2-IP4 by IP6K1/3 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:37468" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855194", "summary": "1-PP-IP5 is phosphorylated to 1,5-(PP)2-IP4 by IP6K1/3 in the cytosol is a Reactome release 97 curated reaction event. The source record connects 1-PP-IP5, ATP to 1,5-(PP)2-IP4, ADP in cytosol and cross-references RHEA:37468.", "equation": "1-PP-IP5 + ATP → 1,5-(PP)2-IP4 + ADP", "inputs": [ { "node_id": "R-ALL-1629740", "reactome_id": "R-ALL-1629740", "label": "1-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:74946", "formula": "C6H6O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629732", "reactome_id": "R-ALL-1629732", "label": "1,5-(PP)2-IP4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77983", "formula": "C6H7O30P8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diphosphoinositol pentakisphosphate kinase activity of IP6K1/3 [cytosol]", "activity_id": "2024000", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:17412958", "title": "A conserved family of enzymes that phosphorylate inositol hexakisphosphate", "journal": "Science", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17412958/" } ], "x": 8018.666666666667, "y": 928.3958333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855195", "label": "I(1,3,4,5,6)P5 transports from the cytosol to the ER lumen", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005829", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855195", "summary": "I(1,3,4,5,6)P5 transports from the cytosol to the ER lumen is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects I(1,3,4,5,6)P5 to I(1,3,4,5,6)P5 in cytosol · endoplasmic reticulum lumen.", "equation": "I(1,3,4,5,6)P5 → I(1,3,4,5,6)P5", "inputs": [ { "node_id": "R-ALL-994141", "reactome_id": "R-ALL-994141", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2024008", "reactome_id": "R-ALL-2024008", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "myo-inositol transmembrane transporter activity of unknown PI transporter [endoplasmic reticulum membrane]", "activity_id": "9954154", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" }, { "id": "PMID:10087200", "title": "Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10087200/" } ], "x": 7461.333333333333, "y": 1074.2708333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855197", "label": "I(1,3,4)P3 is phosphorylated to I(1,3,4,6)P4 by ITPK1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20941" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855197", "summary": "I(1,3,4)P3 is phosphorylated to I(1,3,4,6)P4 by ITPK1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4)P3, ATP to I(1,3,4,6)P4, ADP, H+ in cytosol and cross-references RHEA:20941.", "equation": "I(1,3,4)P3 + ATP → I(1,3,4,6)P4 + ADP + H+", "inputs": [ { "node_id": "R-ALL-994123", "reactome_id": "R-ALL-994123", "label": "I(1,3,4)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58414", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994146", "reactome_id": "R-ALL-994146", "label": "I(1,3,4,6)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57660", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,3,4-trisphosphate 6-kinase activity of ITPK1:Mg2+ [cytosol]", "activity_id": "2023853", "ec_numbers": [ "2.7.1.159" ], "accessions": [] } ], "citations": [ { "id": "PMID:8662638", "title": "Isolation of inositol 1,3,4-trisphosphate 5/6-kinase, cDNA cloning and expression of the recombinant enzyme", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662638/" }, { "id": "PMID:11042108", "title": "Multitasking in signal transduction by a promiscuous human Ins(3,4,5,6)P(4) 1-kinase/Ins(1,3,4)P(3) 5/6-kinase", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11042108/" } ], "x": 7740, "y": 782.5208333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855198", "label": "5-PP-IP5 is dephosphorylated to IP6 by NUDT(1) in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22385" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855198", "summary": "5-PP-IP5 is dephosphorylated to IP6 by NUDT(1) in the cytosol is a Reactome release 97 curated reaction event. The source record connects 5-PP-IP5, H2O to IP6, Pi in cytosol and cross-references RHEA:22385.", "equation": "5-PP-IP5 + H2O → IP6 + Pi", "inputs": [ { "node_id": "R-ALL-1629765", "reactome_id": "R-ALL-1629765", "label": "5-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629770", "reactome_id": "R-ALL-1629770", "label": "IP6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol diphosphate pentakisphosphate diphosphatase activity of NUDT(1) [cytosol]", "activity_id": "2023976", "ec_numbers": [ "3.6.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:10777568", "title": "Discovery of molecular and catalytic diversity among human diphosphoinositol-polyphosphate phosphohydrolases. An expanding Nudt family", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777568/" }, { "id": "PMID:12121577", "title": "Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases", "journal": "BMC Biochem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12121577/" }, { "id": "PMID:12105228", "title": "An adjacent pair of human NUDT genes on chromosome X are preferentially expressed in testis and encode two new isoforms of diphosphoinositol polyphosphate phosphohydrolase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12105228/" }, { "id": "PMID:10419486", "title": "The diadenosine hexaphosphate hydrolases from Schizosaccharomyces pombe and Saccharomyces cerevisiae are homologues of the human diphosphoinositol polyphosphate phosphohydrolase. Overlapping substrate specificities in a MutT-type protein", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419486/" }, { "id": "PMID:9822604", "title": "A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase", "journal": "EMBO J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9822604/" }, { "id": "PMID:10585413", "title": "Site-directed mutagenesis of diphosphoinositol polyphosphate phosphohydrolase, a dual specificity NUDT enzyme that attacks diadenosine polyphosphates and diphosphoinositol polyphosphates", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10585413/" } ], "x": 7461.333333333333, "y": 944.6041666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855200", "label": "I(1,3,4,5)P4 is dephosphorylated to I(1,4,5)P3 by MINPP1 in the ER lumen", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [ "RHEA:77156" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855200", "summary": "I(1,3,4,5)P4 is dephosphorylated to I(1,4,5)P3 by MINPP1 in the ER lumen is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5)P4, H2O to I(1,4,5)P3, Pi in endoplasmic reticulum lumen and cross-references RHEA:77156.", "equation": "I(1,3,4,5)P4 + H2O → I(1,4,5)P3 + Pi", "inputs": [ { "node_id": "R-ALL-2024002", "reactome_id": "R-ALL-2024002", "label": "I(1,3,4,5)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2024016", "reactome_id": "R-ALL-2024016", "label": "I(1,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113551", "reactome_id": "R-ALL-113551", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity of MINPP1 [endoplasmic reticulum lumen]", "activity_id": "2024007", "ec_numbers": null, "accessions": [ "Q9UNW1" ] } ], "citations": [ { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" }, { "id": "PMID:10087200", "title": "Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10087200/" } ], "x": 7740, "y": 831.1458333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855201", "label": "I(1,3,4,6)P4 transports from the cytosol to the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855201", "summary": "I(1,3,4,6)P4 transports from the cytosol to the nucleus is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects I(1,3,4,6)P4 to I(1,3,4,6)P4 in cytosol · nucleoplasm.", "equation": "I(1,3,4,6)P4 → I(1,3,4,6)P4", "inputs": [ { "node_id": "R-ALL-994146", "reactome_id": "R-ALL-994146", "label": "I(1,3,4,6)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57660", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2023892", "reactome_id": "R-ALL-2023892", "label": "I(1,3,4,6)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57660", "formula": "C6H8O18P4", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11909533", "title": "Regulation of Ins(3,4,5,6)P(4) signaling by a reversible kinase/phosphatase", "journal": "Curr Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11909533/" }, { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" } ], "x": 7740, "y": 1074.2708333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855202", "label": "I(3,4)P2 is dephosphorylated to I3P by INPP4A/B in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:43389" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855202", "summary": "I(3,4)P2 is dephosphorylated to I3P by INPP4A/B in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(3,4)P2, H2O to I3P, Pi in cytosol and cross-references RHEA:43389.", "equation": "I(3,4)P2 + H2O → I3P + Pi", "inputs": [ { "node_id": "R-ALL-1629746", "reactome_id": "R-ALL-1629746", "label": "I(3,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83241", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629731", "reactome_id": "R-ALL-1629731", "label": "I3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58401", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-3,4-bisphosphate 4-phosphatase activity of INPP4A/B [cytosol]", "activity_id": "2024028", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:7608176", "title": "The isolation and characterization of cDNA encoding human and rat brain inositol polyphosphate 4-phosphatase", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7608176/" }, { "id": "PMID:9295334", "title": "The cDNA cloning and characterization of inositol polyphosphate 4-phosphatase type II. Evidence for conserved alternative splicing in the 4-phosphatase family", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9295334/" } ], "x": 8018.666666666667, "y": 831.1458333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855203", "label": "5-PP-IP5 transports from the nucleus to the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855203", "summary": "5-PP-IP5 transports from the nucleus to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 5-PP-IP5 to 5-PP-IP5 in nucleoplasm · cytosol.", "equation": "5-PP-IP5 → 5-PP-IP5", "inputs": [ { "node_id": "R-ALL-2023940", "reactome_id": "R-ALL-2023940", "label": "5-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1629765", "reactome_id": "R-ALL-1629765", "label": "5-PP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:17690096", "title": "Cloning and characterization of two human VIP1-like inositol hexakisphosphate and diphosphoinositol pentakisphosphate kinases", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17690096/" } ], "x": 8018.666666666667, "y": 1074.2708333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855205", "label": "I(1,3,4,5)P4 is dephosphorylated to I(1,4,5)P3 by PTEN in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:77156" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855205", "summary": "I(1,3,4,5)P4 is dephosphorylated to I(1,4,5)P3 by PTEN in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5)P4, H2O to I(1,4,5)P3, Pi in cytosol and cross-references RHEA:77156.", "equation": "I(1,3,4,5)P4 + H2O → I(1,4,5)P3 + Pi", "inputs": [ { "node_id": "R-ALL-994170", "reactome_id": "R-ALL-994170", "label": "I(1,3,4,5)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4,5-tetrakisphosphate 3-phosphatase activity of PTEN:Mg2+ [cytosol]", "activity_id": "2023851", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9593664", "title": "The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9593664/" }, { "id": "PMID:10866302", "title": "Functional evaluation of PTEN missense mutations using in vitro phosphoinositide phosphatase assay", "journal": "Cancer Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10866302/" } ], "x": 7461.333333333333, "y": 847.3541666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855206", "label": "I(1,3,4,5)P4 is phosphorylated to I(1,3,4,5,6)P5 by IPMK in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855206", "summary": "I(1,3,4,5)P4 is phosphorylated to I(1,3,4,5,6)P5 by IPMK in the nucleus is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5)P4, ATP to I(1,3,4,5,6)P5, ADP in nucleoplasm.", "equation": "I(1,3,4,5)P4 + ATP → I(1,3,4,5,6)P5 + ADP", "inputs": [ { "node_id": "R-ALL-2023891", "reactome_id": "R-ALL-2023891", "label": "I(1,3,4,5)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023901", "reactome_id": "R-ALL-2023901", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,3,4,5-tetrakisphosphate 6-kinase activity of IPMK [nucleoplasm]", "activity_id": "2023893", "ec_numbers": [ "2.7.1.151" ], "accessions": [ "Q8NFU5" ] } ], "citations": [ { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" }, { "id": "PMID:16293229", "title": "Inositol polyphosphate multikinase regulates inositol 1,4,5,6-tetrakisphosphate", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16293229/" } ], "x": 8018.666666666667, "y": 977.0208333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855207", "label": "IP6 is phosphorylated to 5-PP-IP5 by IP6K1/2 in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:12794" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855207", "summary": "IP6 is phosphorylated to 5-PP-IP5 by IP6K1/2 in the nucleus is a Reactome release 97 curated reaction event. The source record connects IP6, ATP to 5-PP-IP5, ADP in nucleoplasm and cross-references RHEA:12794.", "equation": "IP6 + ATP → 5-PP-IP5 + ADP", "inputs": [ { "node_id": "R-ALL-2023929", "reactome_id": "R-ALL-2023929", "label": "IP6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023940", "reactome_id": "R-ALL-2023940", "label": "5-PP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol hexakisphosphate kinase activity of IP6K1/2 [nucleoplasm]", "activity_id": "2023923", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:17412958", "title": "A conserved family of enzymes that phosphorylate inositol hexakisphosphate", "journal": "Science", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17412958/" }, { "id": "PMID:18355727", "title": "Inositol hexakisphosphate kinase products contain diphosphate and triphosphate groups", "journal": "Chem Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18355727/" } ], "x": 7740, "y": 847.3541666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855208", "label": "I(1,4)P2 is dephosphorylated to I4P by INPP1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15554" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855208", "summary": "I(1,4)P2 is dephosphorylated to I4P by INPP1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,4)P2, H2O to I4P, Pi in cytosol and cross-references RHEA:15554.", "equation": "I(1,4)P2 + H2O → I4P + Pi", "inputs": [ { "node_id": "R-ALL-1629739", "reactome_id": "R-ALL-1629739", "label": "I(1,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58282", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629753", "reactome_id": "R-ALL-1629753", "label": "I4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58469", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,4-bisphosphate 1-phosphatase activity of INPP1:Mg2+ [cytosol]", "activity_id": "2024034", "ec_numbers": [ "3.1.3.57" ], "accessions": [] } ], "citations": [ { "id": "PMID:8390685", "title": "Cloning, heterologous expression, and chromosomal localization of human inositol polyphosphate 1-phosphatase", "journal": "Proc Natl Acad Sci U S A", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8390685/" } ], "x": 8018.666666666667, "y": 847.3541666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855210", "label": "I3P is dephosphorylated to Ins by IMPA1/2 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:30740" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855210", "summary": "I3P is dephosphorylated to Ins by IMPA1/2 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I3P, H2O to Ins, Pi in cytosol and cross-references RHEA:30740.", "equation": "I3P + H2O → Ins + Pi", "inputs": [ { "node_id": "R-ALL-1629731", "reactome_id": "R-ALL-1629731", "label": "I3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58401", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426910", "reactome_id": "R-ALL-426910", "label": "Ins", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17268", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol monophosphate 3-phosphatase activity of IMPA1/2 [cytosol]", "activity_id": "2024053", "ec_numbers": [ "3.1.3.25" ], "accessions": [] } ], "citations": [ { "id": "PMID:17068342", "title": "Spatial expression patterns and biochemical properties distinguish a second myo-inositol monophosphatase IMPA2 from IMPA1", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17068342/" } ], "x": 7461.333333333333, "y": 863.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855211", "label": "I4P is dephosphorylated to Ins by IMPA1/2 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:30736" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855211", "summary": "I4P is dephosphorylated to Ins by IMPA1/2 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I4P, H2O to Ins, Pi in cytosol and cross-references RHEA:30736.", "equation": "I4P + H2O → Ins + Pi", "inputs": [ { "node_id": "R-ALL-1629753", "reactome_id": "R-ALL-1629753", "label": "I4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58469", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426910", "reactome_id": "R-ALL-426910", "label": "Ins", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17268", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol monophosphate 4-phosphatase activity of IMPA1/2 [cytosol]", "activity_id": "2024030", "ec_numbers": [ "3.1.3.25" ], "accessions": [] } ], "citations": [ { "id": "PMID:17068342", "title": "Spatial expression patterns and biochemical properties distinguish a second myo-inositol monophosphatase IMPA2 from IMPA1", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17068342/" } ], "x": 7740, "y": 863.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855212", "label": "1/3-PP-IP5 transports from the cytosol to the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855212", "summary": "1/3-PP-IP5 transports from the cytosol to the nucleus is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 1/3-PP-IP5 to 1/3-PP-IP5 in cytosol · nucleoplasm.", "equation": "1/3-PP-IP5 → 1/3-PP-IP5", "inputs": [ { "node_id": "R-ALL-2023974", "reactome_id": "R-ALL-2023974", "label": "1/3-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2023944", "reactome_id": "R-ALL-2023944", "label": "1/3-PP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:17412958", "title": "A conserved family of enzymes that phosphorylate inositol hexakisphosphate", "journal": "Science", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17412958/" } ], "x": 8018.666666666667, "y": 1090.4791666666665, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855213", "label": "I(1,3,4,5)P4 is dephosphorylated to I(1,3,4)P3 by INPP5B at the plasma membrane", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:11393" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855213", "summary": "I(1,3,4,5)P4 is dephosphorylated to I(1,3,4)P3 by INPP5B at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5)P4, H2O to I(1,3,4)P3, Pi in plasma membrane · cytosol and cross-references RHEA:11393.", "equation": "I(1,3,4,5)P4 + H2O → I(1,3,4)P3 + Pi", "inputs": [ { "node_id": "R-ALL-994170", "reactome_id": "R-ALL-994170", "label": "I(1,3,4,5)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994123", "reactome_id": "R-ALL-994123", "label": "I(1,3,4)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58414", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity of Inositol polyphosphate 5-phosphatase [plasma membrane]", "activity_id": "2023857", "ec_numbers": [ "3.1.3.56" ], "accessions": [] } ], "citations": [ { "id": "PMID:7721860", "title": "Properties of type II inositol polyphosphate 5-phosphatase", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7721860/" }, { "id": "PMID:1718960", "title": "Cloning and expression of human 75-kDa inositol polyphosphate-5-phosphatase", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1718960/" }, { "id": "PMID:15474001", "title": "Type II phosphoinositide 5-phosphatases have unique sensitivities towards fatty acid composition and head group phosphorylation", "journal": "FEBS Lett", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15474001/" } ], "x": 8018.666666666667, "y": 863.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855214", "label": "PL(C)D4:3xCa2+ hydrolse PI(4,5)P2 to I(1,4,5)P3 and DAG at the ER membrane", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:33179", "RHEA:33180" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855214", "summary": "PL(C)D4:3xCa2+ hydrolse PI(4,5)P2 to I(1,4,5)P3 and DAG at the ER membrane is a Reactome release 97 curated reaction event. The source record connects PI(4,5)P2, H2O to DAG, I(1,4,5)P3, H+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:33179, RHEA:33180.", "equation": "PI(4,5)P2 + H2O → DAG + I(1,4,5)P3 + H+", "inputs": [ { "node_id": "R-ALL-2023856", "reactome_id": "R-ALL-2023856", "label": "PI(4,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphatidylinositol-4,5-bisphosphate phospholipase C activity of PL(C)D4:3xCa2+ [endoplasmic reticulum membrane]", "activity_id": "2023871", "ec_numbers": [ "3.1.4.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:15140260", "title": "Phospholipase C delta-4 overexpression upregulates ErbB1/2 expression, Erk signaling pathway, and proliferation in MCF-7 cells", "journal": "Mol Cancer", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15140260/" }, { "id": "PMID:15037625", "title": "The pleckstrin homology domain of phosphoinositide-specific phospholipase Cdelta4 is not a critical determinant of the membrane localization of the enzyme", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15037625/" } ], "x": 8018.666666666667, "y": 782.5208333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855216", "label": "IP6 is phosphorylated to 1-PP-IP5 by PPIP5K1/2 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:37460" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855216", "summary": "IP6 is phosphorylated to 1-PP-IP5 by PPIP5K1/2 in the cytosol is a Reactome release 97 curated reaction event. The source record connects IP6, ATP to 1-PP-IP5, ADP in cytosol and cross-references RHEA:37460.", "equation": "IP6 + ATP → 1-PP-IP5 + ADP", "inputs": [ { "node_id": "R-ALL-1629770", "reactome_id": "R-ALL-1629770", "label": "IP6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629740", "reactome_id": "R-ALL-1629740", "label": "1-PP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:74946", "formula": "C6H6O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol hexakisphosphate kinase activity of PPIP5K1/2 [cytosol]", "activity_id": "2023991", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:17690096", "title": "Cloning and characterization of two human VIP1-like inositol hexakisphosphate and diphosphoinositol pentakisphosphate kinases", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17690096/" }, { "id": "PMID:17412958", "title": "A conserved family of enzymes that phosphorylate inositol hexakisphosphate", "journal": "Science", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17412958/" }, { "id": "PMID:17702752", "title": "Purification, sequencing, and molecular identification of a mammalian PP-InsP5 kinase that is activated when cells are exposed to hyperosmotic stress", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17702752/" }, { "id": "PMID:18981179", "title": "Structural analysis and detection of biological inositol pyrophosphates reveal that the family of VIP/diphosphoinositol pentakisphosphate kinases are 1/3-kinases", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18981179/" }, { "id": "PMID:22119861", "title": "Structural basis for an inositol pyrophosphate kinase surmounting phosphate crowding", "journal": "Nat. Chem. Biol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22119861/" } ], "x": 7740, "y": 944.6041666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855217", "label": "I(1,4,5)P3 transports from the ER lumen to the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "endoplasmic reticulum lumen · cytosol", "compartment_ids": [ "GO:0005788", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855217", "summary": "I(1,4,5)P3 transports from the ER lumen to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects I(1,4,5)P3 to I(1,4,5)P3 in endoplasmic reticulum lumen · cytosol.", "equation": "I(1,4,5)P3 → I(1,4,5)P3", "inputs": [ { "node_id": "R-ALL-2024016", "reactome_id": "R-ALL-2024016", "label": "I(1,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "myo-inositol transmembrane transporter activity of unknown PI transporter [endoplasmic reticulum membrane]", "activity_id": "9954154", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" }, { "id": "PMID:10087200", "title": "Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10087200/" } ], "x": 7461.333333333333, "y": 1090.4791666666665, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855218", "label": "I(1,3,4,5)P4 is dephosphorylated to I(1,3,4)P3 by INPP5[3]/ITPK1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11393" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855218", "summary": "I(1,3,4,5)P4 is dephosphorylated to I(1,3,4)P3 by INPP5[3]/ITPK1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5)P4, H2O to I(1,3,4)P3, Pi in cytosol and cross-references RHEA:11393.", "equation": "I(1,3,4,5)P4 + H2O → I(1,3,4)P3 + Pi", "inputs": [ { "node_id": "R-ALL-994170", "reactome_id": "R-ALL-994170", "label": "I(1,3,4,5)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994123", "reactome_id": "R-ALL-994123", "label": "I(1,3,4)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58414", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity of INPP5(3)/ITPK1 [cytosol]", "activity_id": "2023846", "ec_numbers": [ "3.1.3.56" ], "accessions": [] } ], "citations": [ { "id": "PMID:11909533", "title": "Regulation of Ins(3,4,5,6)P(4) signaling by a reversible kinase/phosphatase", "journal": "Curr Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11909533/" }, { "id": "PMID:15474001", "title": "Type II phosphoinositide 5-phosphatases have unique sensitivities towards fatty acid composition and head group phosphorylation", "journal": "FEBS Lett", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15474001/" }, { "id": "PMID:12223481", "title": "The human homolog of the rat inositol phosphate multikinase is an inositol 1,3,4,6-tetrakisphosphate 5-kinase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12223481/" }, { "id": "PMID:8769125", "title": "Cloning and expression of a human placenta inositol 1,3,4,5-tetrakisphosphate and phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8769125/" }, { "id": "PMID:15316017", "title": "Comparative mechanistic and substrate specificity study of inositol polyphosphate 5-phosphatase Schizosaccharomyces pombe Synaptojanin and SHIP2", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15316017/" }, { "id": "PMID:8723348", "title": "Multiple forms of an inositol polyphosphate 5-phosphatase form signaling complexes with Shc and Grb2", "journal": "Curr Biol", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8723348/" } ], "x": 7461.333333333333, "y": 879.7708333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855219", "label": "I(1,3,4,5,6)P5 is dephosphorylated to I(3,4,5,6)P4 by ITPK1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855219", "summary": "I(1,3,4,5,6)P5 is dephosphorylated to I(3,4,5,6)P4 by ITPK1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5,6)P5, H2O to I(3,4,5,6)P4, Pi in cytosol.", "equation": "I(1,3,4,5,6)P5 + H2O → I(3,4,5,6)P4 + Pi", "inputs": [ { "node_id": "R-ALL-994141", "reactome_id": "R-ALL-994141", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-994149", "reactome_id": "R-ALL-994149", "label": "I(3,4,5,6)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57539", "formula": "C6H8O18P4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4,5,6-pentakisphosphate 1-phosphatase activity of ITPK1:Mg2+ [cytosol]", "activity_id": "2023962", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:11909533", "title": "Regulation of Ins(3,4,5,6)P(4) signaling by a reversible kinase/phosphatase", "journal": "Curr Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11909533/" } ], "x": 7461.333333333333, "y": 993.2291666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855220", "label": "(PP)2-IP4 transports from the nucleus to the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm · cytosol", "compartment_ids": [ "GO:0005654", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855220", "summary": "(PP)2-IP4 transports from the nucleus to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects (PP)2-IP4 to (PP)2-IP4 in nucleoplasm · cytosol.", "equation": "(PP)2-IP4 → (PP)2-IP4", "inputs": [ { "node_id": "R-ALL-2023936", "reactome_id": "R-ALL-2023936", "label": "(PP)2-IP4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2023968", "reactome_id": "R-ALL-2023968", "label": "(PP)2-IP4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "narrow pore channel activity of Nuclear Pore Complex (NPC) [nuclear envelope]", "activity_id": "9954155", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12121577", "title": "Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases", "journal": "BMC Biochem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12121577/" } ], "x": 7461.333333333333, "y": 1106.6875, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-1855221", "label": "PI(4,5)P2 is hydrolysed to I(1,4,5)P3 and DAG by tethered PLC[1] at the plasma membrane", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:33180", "RHEA:33179" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855221", "summary": "PI(4,5)P2 is hydrolysed to I(1,4,5)P3 and DAG by tethered PLC[1] at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(4,5)P2, H2O to DAG, I(1,4,5)P3, H+ in plasma membrane · cytosol and cross-references RHEA:33180, RHEA:33179.", "equation": "PI(4,5)P2 + H2O → DAG + I(1,4,5)P3 + H+", "inputs": [ { "node_id": "R-ALL-179856", "reactome_id": "R-ALL-179856", "label": "PI(4,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-114519", "reactome_id": "R-ALL-114519", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphatidylinositol-4,5-bisphosphate phospholipase C activity of PLCdegh [plasma membrane]", "activity_id": "2023860", "ec_numbers": [ "3.1.4.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:7890667", "title": "Cloning and identification of amino acid residues of human phospholipase C delta 1 essential for catalysis", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7890667/" }, { "id": "PMID:11022047", "title": "A novel bifunctional phospholipase c that is regulated by Galpha 12 and stimulates the Ras/mitogen-activated protein kinase pathway", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11022047/" }, { "id": "PMID:2841328", "title": "Purification and characterization of membrane-bound phospholipase C specific for phosphoinositides from human platelets", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2841328/" }, { "id": "PMID:9360711", "title": "Expression, purification and kinetic properties of human recombinant phospholipase C delta 3", "journal": "Acta Biochim Pol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9360711/" }, { "id": "PMID:19179337", "title": "Phosphorylation of Nephrin Triggers Ca2+ Signaling by Recruitment and Activation of Phospholipase C-{gamma}1", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19179337/" }, { "id": "PMID:15702972", "title": "Molecular cloning and characterization of a novel phospholipase C, PLC-eta", "journal": "Biochem J", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15702972/" } ], "x": 7461.333333333333, "y": 798.7291666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855222", "label": "I(1,4,5)P3 is dephosphorylated to I(1,4)P2 by INPP5A/B at the plasma membrane", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:19798" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855222", "summary": "I(1,4,5)P3 is dephosphorylated to I(1,4)P2 by INPP5A/B at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects I(1,4,5)P3, H2O to I(1,4)P2, Pi in plasma membrane · cytosol and cross-references RHEA:19798.", "equation": "I(1,4,5)P3 + H2O → I(1,4)P2 + Pi", "inputs": [ { "node_id": "R-ALL-114520", "reactome_id": "R-ALL-114520", "label": "I(1,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629739", "reactome_id": "R-ALL-1629739", "label": "I(1,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58282", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,4,5-trisphosphate 5-phosphatase activity of INPP5A/B [plasma membrane]", "activity_id": "2024039", "ec_numbers": [ "3.1.3.56" ], "accessions": [] } ], "citations": [ { "id": "PMID:8006039", "title": "Characterization of a cDNA encoding the 43-kDa membrane-associated inositol-polyphosphate 5-phosphatase", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8006039/" }, { "id": "PMID:7721860", "title": "Properties of type II inositol polyphosphate 5-phosphatase", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7721860/" }, { "id": "PMID:1718960", "title": "Cloning and expression of human 75-kDa inositol polyphosphate-5-phosphatase", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1718960/" }, { "id": "PMID:15474001", "title": "Type II phosphoinositide 5-phosphatases have unique sensitivities towards fatty acid composition and head group phosphorylation", "journal": "FEBS Lett", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15474001/" } ], "x": 7740, "y": 879.7708333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855223", "label": "I(1,3,4,5,6)P5 is phosphorylated to 5-PP-IP4 by IP6K1/3 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855223", "summary": "I(1,3,4,5,6)P5 is phosphorylated to 5-PP-IP4 by IP6K1/3 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,5,6)P5, ATP to 5-PP-IP4, ADP in cytosol.", "equation": "I(1,3,4,5,6)P5 + ATP → 5-PP-IP4 + ADP", "inputs": [ { "node_id": "R-ALL-994141", "reactome_id": "R-ALL-994141", "label": "I(1,3,4,5,6)P5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629755", "reactome_id": "R-ALL-1629755", "label": "5-PP-IP4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62928", "formula": "C6H18O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,3,4,5,6-pentakisphosphate kinase activity of IP6K1/3 [cytosol]", "activity_id": "2023967", "ec_numbers": [ "2.7.4.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:10827188", "title": "The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10827188/" }, { "id": "PMID:18355727", "title": "Inositol hexakisphosphate kinase products contain diphosphate and triphosphate groups", "journal": "Chem Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18355727/" } ], "x": 7740, "y": 993.2291666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855224", "label": "5-PP-IP5 is phosphorylated to 5-PPP-IP5 by IP6K1/2 in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855224", "summary": "5-PP-IP5 is phosphorylated to 5-PPP-IP5 by IP6K1/2 in the nucleus is a Reactome release 97 curated reaction event. The source record connects 5-PP-IP5, ATP to 5-PPP-IP5, ADP in nucleoplasm.", "equation": "5-PP-IP5 + ATP → 5-PPP-IP5 + ADP", "inputs": [ { "node_id": "R-ALL-2023940", "reactome_id": "R-ALL-2023940", "label": "5-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023941", "reactome_id": "R-ALL-2023941", "label": "5-PPP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62985", "formula": "C6H20O30P8", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol 5-diphosphate pentakisphosphate 5-kinase activity of IP6K1/2 [nucleoplasm]", "activity_id": "2023947", "ec_numbers": [ "2.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:18355727", "title": "Inositol hexakisphosphate kinase products contain diphosphate and triphosphate groups", "journal": "Chem Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18355727/" } ], "x": 8018.666666666667, "y": 993.2291666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855225", "label": "IP6 is dephosphorylated to I(1,2,4,5,6)P5 by MINPP1 in the ER lumen", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [ "RHEA:16990" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855225", "summary": "IP6 is dephosphorylated to I(1,2,4,5,6)P5 by MINPP1 in the ER lumen is a Reactome release 97 curated reaction event. The source record connects IP6, H2O to I(1,2,4,5,6)P5, Pi in endoplasmic reticulum lumen and cross-references RHEA:16990.", "equation": "IP6 + H2O → I(1,2,4,5,6)P5 + Pi", "inputs": [ { "node_id": "R-ALL-2024018", "reactome_id": "R-ALL-2024018", "label": "IP6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2564836", "reactome_id": "R-ALL-2564836", "label": "I(1,2,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57798", "formula": "C6H7O21P5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113551", "reactome_id": "R-ALL-113551", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol hexakisphosphate 3-phosphatase activity of MINPP1 [endoplasmic reticulum lumen]", "activity_id": "2024020", "ec_numbers": [ "3.1.3.8" ], "accessions": [ "Q9UNW1" ] } ], "citations": [ { "id": "PMID:1653239", "title": "Purification of an inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase activity from rat liver and the evaluation of its substrate specificity", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1653239/" }, { "id": "PMID:9923613", "title": "The human and rat forms of multiple inositol polyphosphate phosphatase: functional homology with a histidine acid phosphatase up-regulated during endochondral ossification", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9923613/" }, { "id": "PMID:10087200", "title": "Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10087200/" }, { "id": "PMID:15979280", "title": "Physiological levels of PTEN control the size of the cellular Ins(1,3,4,5,6)P(5) pool", "journal": "Cell Signal", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/15979280/" } ], "x": 8018.666666666667, "y": 879.7708333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855227", "label": "IP6 is phosphorylated to 5-PP-IP5 by IP6K1/3 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12794" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855227", "summary": "IP6 is phosphorylated to 5-PP-IP5 by IP6K1/3 in the cytosol is a Reactome release 97 curated reaction event. The source record connects IP6, ATP to 5-PP-IP5, ADP in cytosol and cross-references RHEA:12794.", "equation": "IP6 + ATP → 5-PP-IP5 + ADP", "inputs": [ { "node_id": "R-ALL-1629770", "reactome_id": "R-ALL-1629770", "label": "IP6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629765", "reactome_id": "R-ALL-1629765", "label": "5-PP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58628", "formula": "C6H6O27P7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol hexakisphosphate kinase activity of IP6K1/3 [cytosol]", "activity_id": "2023958", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:17412958", "title": "A conserved family of enzymes that phosphorylate inositol hexakisphosphate", "journal": "Science", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17412958/" }, { "id": "PMID:18355727", "title": "Inositol hexakisphosphate kinase products contain diphosphate and triphosphate groups", "journal": "Chem Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18355727/" }, { "id": "PMID:18981179", "title": "Structural analysis and detection of biological inositol pyrophosphates reveal that the family of VIP/diphosphoinositol pentakisphosphate kinases are 1/3-kinases", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18981179/" } ], "x": 8018.666666666667, "y": 944.6041666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855228", "label": "I(1,3,4,6)P4 is phosphorylated to I(1,3,4,5,6)P5 by IPMK in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:12718" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855228", "summary": "I(1,3,4,6)P4 is phosphorylated to I(1,3,4,5,6)P5 by IPMK in the nucleus is a Reactome release 97 curated reaction event. The source record connects I(1,3,4,6)P4, ATP to I(1,3,4,5,6)P5, ADP, H+ in nucleoplasm and cross-references RHEA:12718.", "equation": "I(1,3,4,6)P4 + ATP → I(1,3,4,5,6)P5 + ADP + H+", "inputs": [ { "node_id": "R-ALL-2023892", "reactome_id": "R-ALL-2023892", "label": "I(1,3,4,6)P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57660", "formula": "C6H8O18P4", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023901", "reactome_id": "R-ALL-2023901", "label": "I(1,3,4,5,6)P5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57733", "formula": "C6H7O21P5", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,3,4,6-tetrakisphosphate 5-kinase activity of IPMK [nucleoplasm]", "activity_id": "2023903", "ec_numbers": [ "2.7.1.140" ], "accessions": [ "Q8NFU5" ] } ], "citations": [ { "id": "PMID:12223481", "title": "The human homolog of the rat inositol phosphate multikinase is an inositol 1,3,4,6-tetrakisphosphate 5-kinase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12223481/" }, { "id": "PMID:16293229", "title": "Inositol polyphosphate multikinase regulates inositol 1,4,5,6-tetrakisphosphate", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16293229/" } ], "x": 7740, "y": 798.7291666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855230", "label": "5-PP-IP4 is phosphorylated to 1,5-(PP)2-IP3 by IP6K1/2 in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1855230", "summary": "5-PP-IP4 is phosphorylated to 1,5-(PP)2-IP3 by IP6K1/2 in the nucleus is a Reactome release 97 curated reaction event. The source record connects 5-PP-IP4, ATP to ADP, 1,5-(PP)2-IP3 in nucleoplasm.", "equation": "5-PP-IP4 + ATP → ADP + 1,5-(PP)2-IP3", "inputs": [ { "node_id": "R-ALL-2023922", "reactome_id": "R-ALL-2023922", "label": "5-PP-IP4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:142939", "formula": "C6H7O24P6", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2023948", "reactome_id": "R-ALL-2023948", "label": "1,5-(PP)2-IP3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62931", "formula": "C6H19O27P7", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "diphosphoinositol tetrakisphosphate kinase activity of IP6K1/2 [nucleoplasm]", "activity_id": "2023930", "ec_numbers": [ "2.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11502751", "title": "Identification and characterization of a novel inositol hexakisphosphate kinase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11502751/" }, { "id": "PMID:10827188", "title": "The inositol hexakisphosphate kinase family. Catalytic flexibility and function in yeast vacuole biogenesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10827188/" }, { "id": "PMID:18355727", "title": "Inositol hexakisphosphate kinase products contain diphosphate and triphosphate groups", "journal": "Chem Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18355727/" } ], "x": 7461.333333333333, "y": 1009.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855232", "label": "I(1,3,4)P3 is dephosphorylated to I(3,4)P2 by INPP1 in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:70320" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855232", "summary": "I(1,3,4)P3 is dephosphorylated to I(3,4)P2 by INPP1 in the cytosol is a Reactome release 97 curated reaction event. The source record connects I(1,3,4)P3, H2O to I(3,4)P2, Pi in cytosol and cross-references RHEA:70320.", "equation": "I(1,3,4)P3 + H2O → I(3,4)P2 + Pi", "inputs": [ { "node_id": "R-ALL-994123", "reactome_id": "R-ALL-994123", "label": "I(1,3,4)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58414", "formula": "C6H9O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629746", "reactome_id": "R-ALL-1629746", "label": "I(3,4)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83241", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol-1,3,4-trisphosphate 1-phosphatase activity of INPP1:Mg2+ [cytosol]", "activity_id": "2024032", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:8390685", "title": "Cloning, heterologous expression, and chromosomal localization of human inositol polyphosphate 1-phosphatase", "journal": "Proc Natl Acad Sci U S A", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8390685/" } ], "x": 7461.333333333333, "y": 895.9791666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1855233", "label": "I(1,4,5)P3 is phosphorylated to I(1,3,4,5)P4 by IPMK in the nucleus", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:11021" ], "source_url": "https://reactome.org/content/detail/R-HSA-1855233", "summary": "I(1,4,5)P3 is phosphorylated to I(1,3,4,5)P4 by IPMK in the nucleus is a Reactome release 97 curated reaction event. The source record connects I(1,4,5)P3, ATP to I(1,3,4,5)P4, ADP, H+ in nucleoplasm and cross-references RHEA:11021.", "equation": "I(1,4,5)P3 + ATP → I(1,3,4,5)P4 + ADP + H+", "inputs": [ { "node_id": "R-ALL-2023907", "reactome_id": "R-ALL-2023907", "label": "I(1,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:203600", "formula": "C6H9O15P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023891", "reactome_id": "R-ALL-2023891", "label": "I(1,3,4,5)P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57895", "formula": "C6H8O18P4", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol-1,4,5-trisphosphate 3-kinase activity of IPMK [nucleoplasm]", "activity_id": "2023913", "ec_numbers": [ "2.7.1.127" ], "accessions": [ "Q8NFU5" ] } ], "citations": [ { "id": "PMID:12027805", "title": "The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12027805/" }, { "id": "PMID:12223481", "title": "The human homolog of the rat inositol phosphate multikinase is an inositol 1,3,4,6-tetrakisphosphate 5-kinase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12223481/" }, { "id": "PMID:16293229", "title": "Inositol polyphosphate multikinase regulates inositol 1,4,5,6-tetrakisphosphate", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16293229/" } ], "x": 8018.666666666667, "y": 798.7291666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1861788", "label": "GBA2 hydrolyzes GlcCer (plasma membrane)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1861788", "summary": "GBA2 hydrolyzes GlcCer (plasma membrane) is a Reactome release 97 curated reaction event. The source record connects GlcCer, H2O to CERA, Glc in plasma membrane · cytosol.", "equation": "GlcCer + H2O → CERA + Glc", "inputs": [ { "node_id": "R-ALL-1861782", "reactome_id": "R-ALL-1861782", "label": "GlcCer", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:22801", "formula": "C25H46NO8R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-429807", "reactome_id": "R-ALL-429807", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70113", "reactome_id": "R-ALL-70113", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucosylceramidase activity of GBA2 [plasma membrane]", "activity_id": "1861763", "ec_numbers": [ "3.2.1.45" ], "accessions": [ "Q9HCG7" ] } ], "citations": [ { "id": "PMID:11489889", "title": "Molecular cloning and expression of human bile acid beta-glucosidase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11489889/" }, { "id": "PMID:17105727", "title": "Identification of the non-lysosomal glucosylceramidase as beta-glucosidase 2", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17105727/" } ], "x": 3321.3333333333335, "y": 1764.9038461538462, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1861789", "label": "GBA3 hydrolyzes GlcCer (cytosol)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1861789", "summary": "GBA3 hydrolyzes GlcCer (cytosol) is a Reactome release 97 curated reaction event. The source record connects GlcCer, H2O to CERA, Glc in cytosol.", "equation": "GlcCer + H2O → CERA + Glc", "inputs": [ { "node_id": "R-ALL-1861764", "reactome_id": "R-ALL-1861764", "label": "GlcCer", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:22801", "formula": "C25H46NO8R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194377", "reactome_id": "R-ALL-194377", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70113", "reactome_id": "R-ALL-70113", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucosylceramidase activity of GBA3 [cytosol]", "activity_id": "1861757", "ec_numbers": [ "3.2.1.45" ], "accessions": [ "Q9H227" ] } ], "citations": [ { "id": "PMID:12594539", "title": "Deglycosylation by small intestinal epithelial cell beta-glucosidases is a critical step in the absorption and metabolism of dietary flavonoid glycosides in humans", "journal": "Eur J Nutr", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12594539/" }, { "id": "PMID:11784319", "title": "Functional expression of human liver cytosolic beta-glucosidase in Pichia pastoris. Insights into its role in the metabolism of dietary glucosides", "journal": "Eur J Biochem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11784319/" }, { "id": "PMID:20728381", "title": "The cytosolic β-glucosidase GBA3 does not influence type 1 Gaucher disease manifestation", "journal": "Blood Cells Mol Dis", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/20728381/" } ], "x": 3600, "y": 1764.9038461538462, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1878002", "label": "XYLTs transfer Xyl to core protein", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:50193" ], "source_url": "https://reactome.org/content/detail/R-HSA-1878002", "summary": "XYLTs transfer Xyl to core protein is a Reactome release 97 curated reaction event. The source record connects UDP-Xyl, GAG core proteins to UDP, xylosyl-core proteins, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:50193.", "equation": "UDP-Xyl + GAG core proteins → UDP + xylosyl-core proteins + H+", "inputs": [ { "node_id": "R-ALL-742361", "reactome_id": "R-ALL-742361", "label": "UDP-Xyl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57632", "formula": "C14H20N2O16P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2054107", "reactome_id": "R-HSA-2054107", "label": "GAG core proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064201", "reactome_id": "R-HSA-2064201", "label": "xylosyl-core proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein xylosyltransferase activity of XYLT1, XYLT2 [Golgi membrane]", "activity_id": "1877999", "ec_numbers": [ "2.4.2.26" ], "accessions": [] } ], "citations": [ { "id": "PMID:15294915", "title": "Analysis of the DXD motifs in human xylosyltransferase I required for enzyme activity", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15294915/" }, { "id": "PMID:15461586", "title": "Human xylosyltransferase I: functional and biochemical characterization of cysteine residues required for enzymic activity", "journal": "Biochem J", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15461586/" }, { "id": "PMID:11099377", "title": "Molecular cloning and expression of human UDP-d-Xylose:proteoglycan core protein beta-d-xylosyltransferase and its first isoform XT-II", "journal": "J Mol Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11099377/" }, { "id": "PMID:17194707", "title": "XT-II, the second isoform of human peptide-O-xylosyltransferase, displays enzymatic activity", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17194707/" } ], "x": 2660, "y": 795.0454545454545, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-188467", "label": "Gly-3-P+FAD-\u003eDHAP+FADH2 (catalyzed by mitochondrial Gly-Phos dehydrogenase)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids", "triglycerides" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:31284" ], "source_url": "https://reactome.org/content/detail/R-HSA-188467", "summary": "Gly-3-P+FAD-\u003eDHAP+FADH2 (catalyzed by mitochondrial Gly-Phos dehydrogenase) is a Reactome release 97 curated reaction event. The source record connects G3P, FAD to DHAP, FADH2 in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:31284.", "equation": "G3P + FAD → DHAP + FADH2", "inputs": [ { "node_id": "R-ALL-188458", "reactome_id": "R-ALL-188458", "label": "G3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-188451", "reactome_id": "R-ALL-188451", "label": "DHAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57642", "formula": "C3H5O6P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerol-3-phosphate dehydrogenase (quinone) activity of GPD2 [mitochondrial inner membrane]", "activity_id": "188456", "ec_numbers": [ "1.1.5.3" ], "accessions": [ "P43304" ] } ], "citations": [ { "id": "PMID:7821823", "title": "The sequence of a human mitochondrial glycerol-3-phosphate dehydrogenase-encoding cDNA", "journal": "Gene", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7821823/" }, { "id": "PMID:8687421", "title": "Molecular cloning of human mitochondrial glycerophosphate dehydrogenase gene: genomic structure, chromosomal localization, and existence of a pseudogene", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8687421/" }, { "id": "PMID:8163052", "title": "Enzymatic, metabolic and secretory patterns in human islets of type 2 (non-insulin-dependent) diabetic patients", "journal": "Diabetologia", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8163052/" } ], "x": 2660, "y": 1631.5821917808219, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1889955", "label": "B3GAT dimers transfer GlcA to tetrasaccharide linker", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:24169" ], "source_url": "https://reactome.org/content/detail/R-HSA-1889955", "summary": "B3GAT dimers transfer GlcA to tetrasaccharide linker is a Reactome release 97 curated reaction event. The source record connects Gal-Gal-Xyl-proteins, UDP-GlcA to UDP, GlcA-Gal-Gal-Xyl-proteins, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:24169.", "equation": "Gal-Gal-Xyl-proteins + UDP-GlcA → UDP + GlcA-Gal-Gal-Xyl-proteins + H+", "inputs": [ { "node_id": "R-HSA-2064214", "reactome_id": "R-HSA-2064214", "label": "Gal-Gal-Xyl-proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-1889970", "reactome_id": "R-ALL-1889970", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064225", "reactome_id": "R-HSA-2064225", "label": "GlcA-Gal-Gal-Xyl-proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity of B3GAT dimers [Golgi membrane]", "activity_id": "1889977", "ec_numbers": [ "2.4.1.135" ], "accessions": [] } ], "citations": [ { "id": "PMID:14993226", "title": "Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14993226/" }, { "id": "PMID:9506957", "title": "Molecular cloning and expression of glucuronyltransferase I involved in the biosynthesis of the glycosaminoglycan-protein linkage region of proteoglycans", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9506957/" }, { "id": "PMID:10842173", "title": "Structure/function of the human Ga1beta1,3-glucuronosyltransferase. Dimerization and functional activity are mediated by two crucial cysteine residues", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10842173/" }, { "id": "PMID:12522689", "title": "Cloning, characterization, and chromosome mapping of the human GlcAT-S gene", "journal": "J Hum Genet", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12522689/" } ], "x": 2938.666666666667, "y": 795.0454545454545, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1889978", "label": "B3GALT6 transfers Gal to the tetrasaccharide linker", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:11781" ], "source_url": "https://reactome.org/content/detail/R-HSA-1889978", "summary": "B3GALT6 transfers Gal to the tetrasaccharide linker is a Reactome release 97 curated reaction event. The source record connects Gal-Xyl-proteins, UDP-Gal to UDP, H+, Gal-Gal-Xyl-proteins in Golgi membrane · Golgi lumen and cross-references RHEA:11781.", "equation": "Gal-Xyl-proteins + UDP-Gal → UDP + H+ + Gal-Gal-Xyl-proteins", "inputs": [ { "node_id": "R-HSA-2064058", "reactome_id": "R-HSA-2064058", "label": "Gal-Xyl-proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-735682", "reactome_id": "R-ALL-735682", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2064214", "reactome_id": "R-HSA-2064214", "label": "Gal-Gal-Xyl-proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "galactosylxylosylprotein 3-beta-galactosyltransferase activity of B3GALT6 [Golgi membrane]", "activity_id": "1889957", "ec_numbers": [ "2.4.1.134" ], "accessions": [ "Q96L58" ] } ], "citations": [ { "id": "PMID:11551958", "title": "Biosynthesis of the linkage region of glycosaminoglycans: cloning and activity of galactosyltransferase II, the sixth member of the beta 1,3-galactosyltransferase family (beta 3GalT6)", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11551958/" }, { "id": "PMID:9892646", "title": "A beta-1,3-N-acetylglucosaminyltransferase with poly-N-acetyllactosamine synthase activity is structurally related to beta-1,3-galactosyltransferases", "journal": "Proc Natl Acad Sci U S A", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9892646/" } ], "x": 2381.3333333333335, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1889981", "label": "B4GALT7 transfers Gal group to xylosyl-unit of the tetrasaccharide linker", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:15298" ], "source_url": "https://reactome.org/content/detail/R-HSA-1889981", "summary": "B4GALT7 transfers Gal group to xylosyl-unit of the tetrasaccharide linker is a Reactome release 97 curated reaction event. The source record connects xylosyl-core proteins, UDP-Gal to Gal-Xyl-proteins, UDP, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:15298.", "equation": "xylosyl-core proteins + UDP-Gal → Gal-Xyl-proteins + UDP + H+", "inputs": [ { "node_id": "R-HSA-2064201", "reactome_id": "R-HSA-2064201", "label": "xylosyl-core proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-735682", "reactome_id": "R-ALL-735682", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2064058", "reactome_id": "R-HSA-2064058", "label": "Gal-Xyl-proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "xylosylprotein 4-beta-galactosyltransferase activity of B4GALT7 [Golgi membrane]", "activity_id": "1889994", "ec_numbers": [ "2.4.1.133" ], "accessions": [ "Q9UBV7" ] } ], "citations": [ { "id": "PMID:10473568", "title": "Cloning and expression of a proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I. A seventh member of the human beta4-galactosyltransferase gene family", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10473568/" }, { "id": "PMID:10506123", "title": "Molecular basis for the progeroid variant of Ehlers-Danlos syndrome. Identification and characterization of two mutations in galactosyltransferase I gene", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10506123/" } ], "x": 2660, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189381", "label": "BIL translocates from the cytosol to the ER lumen", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005829", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-189381", "summary": "BIL translocates from the cytosol to the ER lumen is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects BIL to BIL in cytosol · endoplasmic reticulum lumen.", "equation": "BIL → BIL", "inputs": [ { "node_id": "R-ALL-189386", "reactome_id": "R-ALL-189386", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159151", "reactome_id": "R-ALL-159151", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:4553030", "title": "[Intracellular bilirubin transport and the membrane of the hepatic endoplasmic reticulum: new aspects in the development of transitory bilirubinemia of the newborn]", "journal": "Monatsschr Kinderheilkd", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/4553030/" }, { "id": "PMID:24366439", "title": "Extensive protein-protein interactions involving UDP-glucuronosyltransferase (UGT) 2B7 in human liver microsomes", "journal": "Drug Metab. Pharmacokinet.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24366439/" } ], "x": 6800, "y": 3150.3214285714284, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-189384", "label": "BLVRA:Zn2+, BLVRB reduce BV to BIL", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15795" ], "source_url": "https://reactome.org/content/detail/R-HSA-189384", "summary": "BLVRA:Zn2+, BLVRB reduce BV to BIL is a Reactome release 97 curated reaction event. The source record connects BV, NADPH to BIL, NADP+ in cytosol and cross-references RHEA:15795.", "equation": "BV + NADPH → BIL + NADP+", "inputs": [ { "node_id": "R-ALL-189396", "reactome_id": "R-ALL-189396", "label": "BV", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57991", "formula": "C33H32N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-189386", "reactome_id": "R-ALL-189386", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "biliverdin reductase [NAD(P)H] activity of BLVRA:Zn2+,BLVRB [cytosol]", "activity_id": "189392", "ec_numbers": [ "1.3.1.24" ], "accessions": [] } ], "citations": [ { "id": "PMID:22823425", "title": "Molecular modeling to provide insight into the substrate binding and catalytic mechanism of human biliverdin-IXα reductase", "journal": "J Phys Chem B", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22823425/" }, { "id": "PMID:10620517", "title": "Initial-rate kinetics of the flavin reductase reaction catalysed by human biliverdin-IXbeta reductase (BVR-B)", "journal": "Biochem. J.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10620517/" } ], "x": 7078.666666666667, "y": 2844.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189398", "label": "HMOX1 dimer, HMOX2 cleave heme", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-189398", "summary": "HMOX1 dimer, HMOX2 cleave heme is a Reactome release 97 curated reaction event. The source record connects ferroheme, NADPH, O2 to NADP+, H2O, Fe2+ in cytosol · endoplasmic reticulum membrane.", "equation": "ferroheme + 3 NADPH + 3 O2 → 3 NADP+ + 3 H2O + Fe2+ + CO + BV", "inputs": [ { "node_id": "R-ALL-71185", "reactome_id": "R-ALL-71185", "label": "ferroheme", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60344", "formula": "C34H30FeN4O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-71067", "reactome_id": "R-ALL-71067", "label": "Fe2+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-189385", "reactome_id": "R-ALL-189385", "label": "CO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17245", "formula": "CO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-189396", "reactome_id": "R-ALL-189396", "label": "BV", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57991", "formula": "C33H32N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "heme oxygenase (decyclizing) activity of HMOX1 dimer, HMOX2 [endoplasmic reticulum membrane]", "activity_id": "189379", "ec_numbers": [ "1.14.14.18" ], "accessions": [] } ], "citations": [], "x": 6521.333333333333, "y": 2955.8214285714284, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189406", "label": "4 PBGs bind to form HMB", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13186" ], "source_url": "https://reactome.org/content/detail/R-HSA-189406", "summary": "4 PBGs bind to form HMB is a Reactome release 97 curated reaction event. The source record connects PBG, H2O to HMBL, NH4+ in cytosol and cross-references RHEA:13186.", "equation": "4 PBG + H2O → HMBL + 4 NH4+", "inputs": [ { "node_id": "R-ALL-189464", "reactome_id": "R-ALL-189464", "label": "PBG", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:58126", "formula": "C10H13N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-189449", "reactome_id": "R-ALL-189449", "label": "HMBL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57845", "formula": "C40H38N4O17", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydroxymethylbilane synthase activity of HMBS:DIPY [cytosol]", "activity_id": "189486", "ec_numbers": [ "2.5.1.61" ], "accessions": [] } ], "citations": [ { "id": "PMID:12773194", "title": "Human porphobilinogen deaminase mutations in the investigation of the mechanism of dipyrromethane cofactor assembly and tetrapyrrole formation", "journal": "Biochem Soc Trans", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12773194/" }, { "id": "PMID:7354069", "title": "Purification and properties of uroporphyrinogen I synthase from human erythrocytes. Identification of stable enzyme-substrate intermediates.", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/7354069/" } ], "x": 6521.333333333333, "y": 2872.464285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189421", "label": "CPO transforms COPRO3 to PPGEN9", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "mitochondrial intermembrane space", "compartment_ids": [ "GO:0005758" ], "rhea_ids": [ "RHEA:18258" ], "source_url": "https://reactome.org/content/detail/R-HSA-189421", "summary": "CPO transforms COPRO3 to PPGEN9 is a Reactome release 97 curated reaction event. The source record connects COPRO3, O2, H+ to PPGEN9, CO2, H2O in mitochondrial intermembrane space and cross-references RHEA:18258.", "equation": "COPRO3 + O2 + H+ → PPGEN9 + 2 CO2 + H2O", "inputs": [ { "node_id": "R-ALL-189411", "reactome_id": "R-ALL-189411", "label": "COPRO3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57309", "formula": "C36H40N4O8", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-189461", "reactome_id": "R-ALL-189461", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-163953", "reactome_id": "R-ALL-163953", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-189478", "reactome_id": "R-ALL-189478", "label": "PPGEN9", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57307", "formula": "C34H38N4O4", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-189480", "reactome_id": "R-ALL-189480", "label": "CO2", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-189422", "reactome_id": "R-ALL-189422", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "coproporphyrinogen oxidase activity of 2xCPO [mitochondrial intermembrane space]", "activity_id": "189416", "ec_numbers": [ "1.3.3.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:16258391", "title": "Kinetic evaluation of human cloned coproporphyrinogen oxidase using a ring isomer of the natural substrate", "journal": "Med Sci Monit", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16258391/" }, { "id": "PMID:666752", "title": "Evidence that the coproporphyrinogen oxidase activity of rat liver is situated in the intermembrane space of mitochondria", "journal": "Biochem J", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/666752/" }, { "id": "PMID:16176984", "title": "Structural basis of hereditary coproporphyria", "journal": "Proc Natl Acad Sci U S A", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16176984/" }, { "id": "PMID:8159699", "title": "Molecular cloning, sequencing, and functional expression of a cDNA encoding human coproporphyrinogen oxidase", "journal": "Proc Natl Acad Sci U S A", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8159699/" } ], "x": 7078.666666666667, "y": 2816.8928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189423", "label": "PPO oxidises PPGEN9 to PRIN9", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "mitochondrial intermembrane space", "compartment_ids": [ "GO:0005758" ], "rhea_ids": [ "RHEA:25577" ], "source_url": "https://reactome.org/content/detail/R-HSA-189423", "summary": "PPO oxidises PPGEN9 to PRIN9 is a Reactome release 97 curated reaction event. The source record connects PPGEN9, O2 to PRIN9, H2O2 in mitochondrial intermembrane space and cross-references RHEA:25577.", "equation": "PPGEN9 + 3 O2 → PRIN9 + 3 H2O2", "inputs": [ { "node_id": "R-ALL-189478", "reactome_id": "R-ALL-189478", "label": "PPGEN9", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57307", "formula": "C34H38N4O4", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-189461", "reactome_id": "R-ALL-189461", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189463", "reactome_id": "R-ALL-189463", "label": "PRIN9", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57306", "formula": "C34H32N4O4", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-189481", "reactome_id": "R-ALL-189481", "label": "H2O2", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxygen-dependent protoporphyrinogen oxidase activity of 2xPPOX:FAD [mitochondrial intermembrane space]", "activity_id": "189420", "ec_numbers": [ "1.3.3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:8771201", "title": "Human protoporphyrinogen oxidase: expression, purification, and characterization of the cloned enzyme", "journal": "Protein Sci", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8771201/" }, { "id": "PMID:23467411", "title": "Quantitative structural insight into human variegate porphyria disease", "journal": "J Biol Chem", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23467411/" } ], "x": 6800, "y": 2872.464285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189425", "label": "UROD decarboxylates URO3 to COPRO3", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19866" ], "source_url": "https://reactome.org/content/detail/R-HSA-189425", "summary": "UROD decarboxylates URO3 to COPRO3 is a Reactome release 97 curated reaction event. The source record connects URO3, H+ to COPRO3, CO2 in cytosol and cross-references RHEA:19866.", "equation": "URO3 + 4 H+ → COPRO3 + 4 CO2", "inputs": [ { "node_id": "R-ALL-189407", "reactome_id": "R-ALL-189407", "label": "URO3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57308", "formula": "C40H36N4O16", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-189455", "reactome_id": "R-ALL-189455", "label": "COPRO3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57309", "formula": "C36H40N4O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbon-carbon lyase activity of 2xUROD [cytosol]", "activity_id": "189412", "ec_numbers": [ "4.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6822570", "title": "Purification and properties of uroporphyrinogen decarboxylase from human erythrocytes. A single enzyme catalyzing the four sequential decarboxylations of uroporphyrinogens I and III.", "journal": "J Biol Chem", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6822570/" }, { "id": "PMID:8644733", "title": "Uroporphyrinogen decarboxylase: complete human gene sequence and molecular study of three families with hepatoerythropoietic porphyria", "journal": "Am J Hum Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8644733/" } ], "x": 7078.666666666667, "y": 2872.464285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189439", "label": "ALAD condenses 2 dALAs to form PBG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24065" ], "source_url": "https://reactome.org/content/detail/R-HSA-189439", "summary": "ALAD condenses 2 dALAs to form PBG is a Reactome release 97 curated reaction event. The source record connects dALA to PBG, H2O, H+ in cytosol and cross-references RHEA:24065.", "equation": "2 dALA → PBG + 2 H2O + H+", "inputs": [ { "node_id": "R-ALL-189489", "reactome_id": "R-ALL-189489", "label": "dALA", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:356416", "formula": "C5H9NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189464", "reactome_id": "R-ALL-189464", "label": "PBG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58126", "formula": "C10H13N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "porphobilinogen synthase activity of 8x(ALAD:Zn2+) [cytosol]", "activity_id": "189415", "ec_numbers": [ "4.2.1.24" ], "accessions": [] } ], "citations": [ { "id": "PMID:11032841", "title": "Mechanistic implications of mutations to the active site lysine of porphobilinogen synthase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11032841/" }, { "id": "PMID:10706561", "title": "Novel molecular defects of the delta-aminolevulinate dehydratase gene in a patient with inherited acute hepatic porphyria", "journal": "Hepatology", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10706561/" } ], "x": 6521.333333333333, "y": 2900.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189442", "label": "ALAS condenses SUCC-CoA and Gly to form dALA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12922" ], "source_url": "https://reactome.org/content/detail/R-HSA-189442", "summary": "ALAS condenses SUCC-CoA and Gly to form dALA is a Reactome release 97 curated reaction event. The source record connects SUCC-CoA, Gly, H+ to dALA, CoA-SH, CO2 in mitochondrial matrix and cross-references RHEA:12922.", "equation": "SUCC-CoA + Gly + H+ → dALA + CoA-SH + CO2", "inputs": [ { "node_id": "R-ALL-70958", "reactome_id": "R-ALL-70958", "label": "SUCC-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57292", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-189466", "reactome_id": "R-ALL-189466", "label": "dALA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:356416", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5-aminolevulinate synthase activity of ALAS1,2 [mitochondrial matrix]", "activity_id": "189479", "ec_numbers": [ "2.3.1.37" ], "accessions": [] } ], "citations": [ { "id": "PMID:1939222", "title": "Biosynthesis of delta-aminolevulinic acid and the regulation of heme formation by immature erythroid cells in man", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1939222/" }, { "id": "PMID:16121195", "title": "Crystal structure of 5-aminolevulinate synthase, the first enzyme of heme biosynthesis, and its link to XLSA in humans", "journal": "EMBO J", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16121195/" } ], "x": 6521.333333333333, "y": 2844.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-189456", "label": "Mitochondrial dALA translocates to cytosol", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "mitochondrial outer membrane", "compartment_ids": [ "GO:0005741" ], "rhea_ids": [ "RHEA:64816" ], "source_url": "https://reactome.org/content/detail/R-HSA-189456", "summary": "Mitochondrial dALA translocates to cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects dALA to dALA in mitochondrial outer membrane and cross-references RHEA:64816.", "equation": "dALA → dALA", "inputs": [ { "node_id": "R-ALL-189466", "reactome_id": "R-ALL-189466", "label": "dALA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:356416", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189489", "reactome_id": "R-ALL-189489", "label": "dALA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:356416", "formula": "C5H9NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:23720443", "title": "ATP-binding cassette B10 regulates early steps of heme synthesis", "journal": "Circ. Res.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23720443/" }, { "id": "PMID:26053025", "title": "ATP Binding and Hydrolysis Properties of ABCB10 and Their Regulation by Glutathione", "journal": "PLoS ONE", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26053025/" } ], "x": 7078.666666666667, "y": 2928.035714285714, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-189457", "label": "Mitochondrial intermembrane PRIN9 translocates to mitochondrial matrix", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-189457", "summary": "Mitochondrial intermembrane PRIN9 translocates to mitochondrial matrix is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PRIN9 to PRIN9 in mitochondrial inner membrane.", "equation": "PRIN9 → PRIN9", "inputs": [ { "node_id": "R-ALL-189463", "reactome_id": "R-ALL-189463", "label": "PRIN9", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57306", "formula": "C34H32N4O4", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189487", "reactome_id": "R-ALL-189487", "label": "PRIN9", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57306", "formula": "C34H32N4O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:17006453", "title": "Identification of a mammalian mitochondrial porphyrin transporter", "journal": "Nature", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17006453/" } ], "x": 7078.666666666667, "y": 3150.3214285714284, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-189465", "label": "FECH binds Fe2+ to PRIN9 to form heme", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-189465", "summary": "FECH binds Fe2+ to PRIN9 to form heme is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PRIN9, Fe2+ to H+, heme b in mitochondrial matrix.", "equation": "PRIN9 + Fe2+ → 2 H+ + heme b", "inputs": [ { "node_id": "R-ALL-189487", "reactome_id": "R-ALL-189487", "label": "PRIN9", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57306", "formula": "C34H32N4O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113531", "reactome_id": "R-ALL-113531", "label": "Fe2+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9853511", "reactome_id": "R-ALL-9853511", "label": "heme b", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:26355", "formula": "C34H32FeN4O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "protoporphyrin ferrochelatase activity of 2x(FECH:2Fe-2S cluster) [mitochondrial inner membrane]", "activity_id": "189476", "ec_numbers": [ "4.98.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:11175906", "title": "The 2.0 A structure of human ferrochelatase, the terminal enzyme of heme biosynthesis.", "journal": "Nat Struct Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11175906/" } ], "x": 7078.666666666667, "y": 2983.6071428571427, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-189467", "label": "Cytosolic COPRO3 translocates to mitochondrial intermembrane space", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005829", "GO:0005758" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-189467", "summary": "Cytosolic COPRO3 translocates to mitochondrial intermembrane space is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects COPRO3 to COPRO3 in cytosol · mitochondrial intermembrane space.", "equation": "COPRO3 → COPRO3", "inputs": [ { "node_id": "R-ALL-189455", "reactome_id": "R-ALL-189455", "label": "COPRO3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57309", "formula": "C36H40N4O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189411", "reactome_id": "R-ALL-189411", "label": "COPRO3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57309", "formula": "C36H40N4O8", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:31872", "title": "The mitochondrial localization of coproporphyrinogen III oxidase", "journal": "Biochem. J.", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/31872/" } ], "x": 6521.333333333333, "y": 3178.1071428571427, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-189488", "label": "UROS transforms HMB to URO3", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18966" ], "source_url": "https://reactome.org/content/detail/R-HSA-189488", "summary": "UROS transforms HMB to URO3 is a Reactome release 97 curated reaction event. The source record connects HMBL to URO3, H2O in cytosol and cross-references RHEA:18966.", "equation": "HMBL → URO3 + H2O", "inputs": [ { "node_id": "R-ALL-189449", "reactome_id": "R-ALL-189449", "label": "HMBL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57845", "formula": "C40H38N4O17", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189407", "reactome_id": "R-ALL-189407", "label": "URO3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57308", "formula": "C40H36N4O16", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "uroporphyrinogen-III synthase activity of UROS [cytosol]", "activity_id": "189468", "ec_numbers": [ "4.2.1.75" ], "accessions": [ "P10746" ] } ], "citations": [ { "id": "PMID:7592565", "title": "Porphobilinogen deaminase and uroporphyrinogen III synthase: structure, molecular biology, and mechanism", "journal": "J Bioenerg Biomembr", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7592565/" }, { "id": "PMID:3805019", "title": "Purification and properties of uroporphyrinogen III synthase from human erythrocytes", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3805019/" } ], "x": 7078.666666666667, "y": 2900.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-190141", "label": "ALAD binds to Pb2+", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-190141", "summary": "ALAD binds to Pb2+ is a Reactome release 97 curated reaction event. The source record connects 8x(ALAD:Zn2+), Pb2+ to 8xALAD:Pb2+:Zn2+, Zn2+ in cytosol.", "equation": "8x(ALAD:Zn2+) + 4 Pb2+ → 8xALAD:Pb2+:Zn2+ + 4 Zn2+", "inputs": [ { "node_id": "R-HSA-189400", "reactome_id": "R-HSA-189400", "label": "8x(ALAD:Zn2+)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-190173", "reactome_id": "R-ALL-190173", "label": "Pb2+", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:27889", "formula": "Pb", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-190145", "reactome_id": "R-HSA-190145", "label": "8xALAD:Pb2+:Zn2+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29426", "reactome_id": "R-ALL-29426", "label": "Zn2+", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:29105", "formula": "Zn", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lead ion binding activity of ALAD [cytosol]", "activity_id": "9955860", "ec_numbers": null, "accessions": [ "P13716" ] } ], "citations": [ { "id": "PMID:11032836", "title": "The molecular mechanism of lead inhibition of human porphobilinogen synthase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11032836/" } ], "x": 6800, "y": 3039.1785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-190168", "label": "HMBL spontaneously transforms to URO1", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-190168", "summary": "HMBL spontaneously transforms to URO1 is a Reactome release 97 curated reaction event. The source record connects HMBL to H2O, URO1 in cytosol.", "equation": "HMBL → H2O + URO1", "inputs": [ { "node_id": "R-ALL-189449", "reactome_id": "R-ALL-189449", "label": "HMBL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57845", "formula": "C40H38N4O17", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-190157", "reactome_id": "R-ALL-190157", "label": "URO1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62626", "formula": "C40H36N4O16", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:3805019", "title": "Purification and properties of uroporphyrinogen III synthase from human erythrocytes", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3805019/" } ], "x": 6521.333333333333, "y": 3122.535714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-190182", "label": "UROD decarboxylates URO1 to COPRO1", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:31240" ], "source_url": "https://reactome.org/content/detail/R-HSA-190182", "summary": "UROD decarboxylates URO1 to COPRO1 is a Reactome release 97 curated reaction event. The source record connects URO1, H+ to COPRO1, CO2 in cytosol and cross-references RHEA:31240.", "equation": "URO1 + 4 H+ → COPRO1 + 4 CO2", "inputs": [ { "node_id": "R-ALL-190157", "reactome_id": "R-ALL-190157", "label": "URO1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62626", "formula": "C40H36N4O16", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-190128", "reactome_id": "R-ALL-190128", "label": "COPRO1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62631", "formula": "C36H40N4O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbon-carbon lyase activity of 2xUROD [cytosol]", "activity_id": "189412", "ec_numbers": [ "4.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6822570", "title": "Purification and properties of uroporphyrinogen decarboxylase from human erythrocytes. A single enzyme catalyzing the four sequential decarboxylations of uroporphyrinogens I and III.", "journal": "J Biol Chem", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6822570/" }, { "id": "PMID:8644733", "title": "Uroporphyrinogen decarboxylase: complete human gene sequence and molecular study of three families with hepatoerythropoietic porphyria", "journal": "Am J Hum Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8644733/" } ], "x": 6800, "y": 2900.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191299", "label": "Squalene is oxidized to its epoxide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:25282" ], "source_url": "https://reactome.org/content/detail/R-HSA-191299", "summary": "Squalene is oxidized to its epoxide is a Reactome release 97 curated reaction event. The source record connects SQNE, NADPH, H+ to SQOX, NADP+, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:25282.", "equation": "SQNE + NADPH + H+ + O2 → SQOX + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-191269", "reactome_id": "R-ALL-191269", "label": "SQNE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15440", "formula": "C30H50", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-191309", "reactome_id": "R-ALL-191309", "label": "SQOX", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15441", "formula": "C30H50O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "squalene monooxygenase activity of SQLE:FAD [endoplasmic reticulum membrane]", "activity_id": "191350", "ec_numbers": [ "1.14.14.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:10666321", "title": "Cloning, heterologous expression, and enzymological characterization of human squalene monooxygenase", "journal": "Arch Biochem Biophys", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10666321/" } ], "x": 2381.3333333333335, "y": 2310.2333333333336, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191303", "label": "FDPS dimer transfers IPPP to GPP", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19362" ], "source_url": "https://reactome.org/content/detail/R-HSA-191303", "summary": "FDPS dimer transfers IPPP to GPP is a Reactome release 97 curated reaction event. The source record connects GPP, IPPP to FAPP, PPi in cytosol and cross-references RHEA:19362.", "equation": "GPP + IPPP → FAPP + PPi", "inputs": [ { "node_id": "R-ALL-191409", "reactome_id": "R-ALL-191409", "label": "GPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58057", "formula": "C10H17O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-191362", "reactome_id": "R-ALL-191362", "label": "IPPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:128769", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-191385", "reactome_id": "R-ALL-191385", "label": "FAPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:175763", "formula": "C15H25O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(2E,6E)-farnesyl diphosphate synthase activity of holo-FDPS dimer [cytosol]", "activity_id": "981559", "ec_numbers": [ "2.5.1.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:10026212", "title": "Human geranylgeranyl diphosphate synthase. cDNA cloning and expression.", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10026212/" }, { "id": "PMID:9741684", "title": "Human geranylgeranyl diphosphate synthase: isolation of the cDNA, chromosomal mapping and tissue expression", "journal": "J Lipid Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9741684/" }, { "id": "PMID:16684881", "title": "The molecular mechanism of nitrogen-containing bisphosphonates as antiosteoporosis drugs", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16684881/" } ], "x": 2938.666666666667, "y": 2180.5666666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191322", "label": "FDPS dimer transfers IPPP to DMAPP", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22409" ], "source_url": "https://reactome.org/content/detail/R-HSA-191322", "summary": "FDPS dimer transfers IPPP to DMAPP is a Reactome release 97 curated reaction event. The source record connects DMAPP, IPPP to GPP, PPi in cytosol and cross-references RHEA:22409.", "equation": "DMAPP + IPPP → GPP + PPi", "inputs": [ { "node_id": "R-ALL-191324", "reactome_id": "R-ALL-191324", "label": "DMAPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57623", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-191362", "reactome_id": "R-ALL-191362", "label": "IPPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:128769", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-191409", "reactome_id": "R-ALL-191409", "label": "GPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58057", "formula": "C10H17O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dimethylallyltranstransferase activity of holo-FDPS dimer [cytosol]", "activity_id": "981571", "ec_numbers": [ "2.5.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10026212", "title": "Human geranylgeranyl diphosphate synthase. cDNA cloning and expression.", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10026212/" }, { "id": "PMID:9741684", "title": "Human geranylgeranyl diphosphate synthase: isolation of the cDNA, chromosomal mapping and tissue expression", "journal": "J Lipid Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9741684/" }, { "id": "PMID:16684881", "title": "The molecular mechanism of nitrogen-containing bisphosphonates as antiosteoporosis drugs", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16684881/" }, { "id": "PMID:33246356", "title": "Structure, catalysis, and inhibition mechanism of prenyltransferase", "journal": "IUBMB Life", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33246356/" } ], "x": 2381.3333333333335, "y": 2206.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191323", "label": "HMGCS1 condenses Ac-CoA and ACA-CoA to form bHMG-CoA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10189" ], "source_url": "https://reactome.org/content/detail/R-HSA-191323", "summary": "HMGCS1 condenses Ac-CoA and ACA-CoA to form bHMG-CoA is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, ACA-CoA, H2O to CoA-SH, bHMG-CoA, H+ in cytosol and cross-references RHEA:10189.", "equation": "Ac-CoA + ACA-CoA + H2O → CoA-SH + bHMG-CoA + H+", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-191293", "reactome_id": "R-ALL-191293", "label": "ACA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57286", "formula": "C25H36N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-191374", "reactome_id": "R-ALL-191374", "label": "bHMG-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43074", "formula": "C27H39N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "hydroxymethylglutaryl-CoA synthase activity of HMGCS1 [cytosol]", "activity_id": "191334", "ec_numbers": [ "2.3.3.10" ], "accessions": [ "Q01581" ] } ], "citations": [ { "id": "PMID:7913309", "title": "Human cytoplasmic 3-hydroxy-3-methylglutaryl coenzyme A synthase: expression, purification, and characterization of recombinant wild-type and Cys129 mutant enzymes", "journal": "Arch Biochem Biophys", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7913309/" } ], "x": 2660, "y": 2024.9666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191352", "label": "HMGCR dimer reduces bHMG-CoA to MVA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:15991" ], "source_url": "https://reactome.org/content/detail/R-HSA-191352", "summary": "HMGCR dimer reduces bHMG-CoA to MVA is a Reactome release 97 curated reaction event. The source record connects bHMG-CoA, NADPH, H+ to MVA, CoA-SH, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:15991.", "equation": "bHMG-CoA + 2 NADPH + 2 H+ → MVA + CoA-SH + 2 NADP+", "inputs": [ { "node_id": "R-ALL-191374", "reactome_id": "R-ALL-191374", "label": "bHMG-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43074", "formula": "C27H39N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-191424", "reactome_id": "R-ALL-191424", "label": "MVA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36464", "formula": "C6H11O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "hydroxymethylglutaryl-CoA reductase (NADPH) activity of HMGCR dimer [endoplasmic reticulum membrane]", "activity_id": "191301", "ec_numbers": [ "1.1.1.34" ], "accessions": [] } ], "citations": [ { "id": "PMID:1967820", "title": "Regulation of the mevalonate pathway", "journal": "Nature", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/1967820/" }, { "id": "PMID:10698924", "title": "Crystal structure of the catalytic portion of human HMG-CoA reductase: insights into regulation of activity and catalysis", "journal": "EMBO J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10698924/" } ], "x": 2938.666666666667, "y": 2024.9666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191366", "label": "Squalene 2,3-epoxide cyclizes, forming lanosterol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:14621", "RHEA:14622" ], "source_url": "https://reactome.org/content/detail/R-HSA-191366", "summary": "Squalene 2,3-epoxide cyclizes, forming lanosterol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects SQOX to LAN in cytosol · endoplasmic reticulum membrane and cross-references RHEA:14621, RHEA:14622.", "equation": "SQOX → LAN", "inputs": [ { "node_id": "R-ALL-191309", "reactome_id": "R-ALL-191309", "label": "SQOX", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15441", "formula": "C30H50O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194634", "reactome_id": "R-ALL-194634", "label": "LAN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16521", "formula": "C30H50O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lanosterol synthase activity of LSS [endoplasmic reticulum membrane]", "activity_id": "191416", "ec_numbers": [ "5.4.99.7" ], "accessions": [ "P48449" ] } ], "citations": [ { "id": "PMID:14766201", "title": "The monotopic membrane protein human oxidosqualene cyclase is active as monomer", "journal": "Biochem Biophys Res Commun", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14766201/" }, { "id": "PMID:8593458", "title": "Molecular cloning of cDNA encoding human lanosterol synthase", "journal": "Biol Pharm Bull", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8593458/" }, { "id": "PMID:5918048", "title": "Enzymic conversion of squalene 2,3-oxide to lanosterol and cholesterol", "journal": "J Am Chem Soc", "year": 1966, "url": "https://pubmed.ncbi.nlm.nih.gov/5918048/" } ], "x": 2381.3333333333335, "y": 2258.366666666667, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-191380", "label": "Mevalonate is phosphorylated to mevalonate-5-phosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17066" ], "source_url": "https://reactome.org/content/detail/R-HSA-191380", "summary": "Mevalonate is phosphorylated to mevalonate-5-phosphate is a Reactome release 97 curated reaction event. The source record connects MVA, ATP to MVA5P, ADP, H+ in cytosol and cross-references RHEA:17066.", "equation": "MVA + ATP → MVA5P + ADP + H+", "inputs": [ { "node_id": "R-ALL-191424", "reactome_id": "R-ALL-191424", "label": "MVA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36464", "formula": "C6H11O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-191296", "reactome_id": "R-ALL-191296", "label": "MVA5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58146", "formula": "C6H10O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "mevalonate kinase activity of MVK dimer [cytosol]", "activity_id": "191389", "ec_numbers": [ "2.7.1.36" ], "accessions": [] } ], "citations": [ { "id": "PMID:14730012", "title": "Mevalonate kinase is a cytosolic enzyme in humans", "journal": "J Cell Sci", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14730012/" }, { "id": "PMID:11111075", "title": "Biochemical and genetic aspects of mevalonate kinase and its deficiency", "journal": "Biochim Biophys Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11111075/" }, { "id": "PMID:1377680", "title": "Molecular cloning of human mevalonate kinase and identification of a missense mutation in the genetic disease mevalonic aciduria", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1377680/" } ], "x": 2660, "y": 2050.9, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191382", "label": "Isopentenyl pyrophosphate rearranges to dimethylallyl pyrophosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23285" ], "source_url": "https://reactome.org/content/detail/R-HSA-191382", "summary": "Isopentenyl pyrophosphate rearranges to dimethylallyl pyrophosphate is a Reactome release 97 curated reaction event. The source record connects IPPP to DMAPP in cytosol and cross-references RHEA:23285.", "equation": "IPPP → DMAPP", "inputs": [ { "node_id": "R-ALL-191362", "reactome_id": "R-ALL-191362", "label": "IPPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:128769", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-191324", "reactome_id": "R-ALL-191324", "label": "DMAPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57623", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "isopentenyl-diphosphate delta-isomerase activity of IDI1 or 2 [cytosol]", "activity_id": "191346", "ec_numbers": [ "5.3.3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8806705", "title": "Human isopentenyl diphosphate: dimethylallyl diphosphate isomerase: overproduction, purification, and characterization", "journal": "Arch Biochem Biophys", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8806705/" } ], "x": 2660, "y": 2258.366666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191402", "label": "Reduction of presqualene diphosphate to form squalene", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:22233" ], "source_url": "https://reactome.org/content/detail/R-HSA-191402", "summary": "Reduction of presqualene diphosphate to form squalene is a Reactome release 97 curated reaction event. The source record connects PSQPP, H+, NADPH to SQNE, NADP+, PPi in cytosol · endoplasmic reticulum membrane and cross-references RHEA:22233.", "equation": "PSQPP + H+ + NADPH → SQNE + NADP+ + PPi", "inputs": [ { "node_id": "R-ALL-191265", "reactome_id": "R-ALL-191265", "label": "PSQPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57310", "formula": "C30H49O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-191269", "reactome_id": "R-ALL-191269", "label": "SQNE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15440", "formula": "C30H50", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "squalene synthase [NAD(P)H] activity of FDFT:Mg2+ [endoplasmic reticulum membrane]", "activity_id": "191305", "ec_numbers": [ "2.5.1.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:10896663", "title": "Crystal structure of human squalene synthase. A key enzyme in cholesterol biosynthesis.", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10896663/" } ], "x": 2381.3333333333335, "y": 2050.9, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191405", "label": "Two FAPP molecules dimerize to form presqualene diphosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:22673" ], "source_url": "https://reactome.org/content/detail/R-HSA-191405", "summary": "Two FAPP molecules dimerize to form presqualene diphosphate is a Reactome release 97 curated reaction event. The source record connects FAPP to PPi, PSQPP in cytosol · endoplasmic reticulum membrane and cross-references RHEA:22673.", "equation": "2 FAPP → PPi + PSQPP", "inputs": [ { "node_id": "R-ALL-191385", "reactome_id": "R-ALL-191385", "label": "FAPP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:175763", "formula": "C15H25O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-191265", "reactome_id": "R-ALL-191265", "label": "PSQPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57310", "formula": "C30H49O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "squalene synthase [NAD(P)H] activity of FDFT:Mg2+ [endoplasmic reticulum membrane]", "activity_id": "191305", "ec_numbers": [ "2.5.1.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:10896663", "title": "Crystal structure of human squalene synthase. A key enzyme in cholesterol biosynthesis.", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10896663/" } ], "x": 2938.666666666667, "y": 2232.4333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191414", "label": "MVD decarboxylates MVA5PP to IPPP", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23733" ], "source_url": "https://reactome.org/content/detail/R-HSA-191414", "summary": "MVD decarboxylates MVA5PP to IPPP is a Reactome release 97 curated reaction event. The source record connects MVA5PP, ATP to IPPP, ADP, Pi in cytosol and cross-references RHEA:23733.", "equation": "MVA5PP + ATP → IPPP + ADP + Pi", "inputs": [ { "node_id": "R-ALL-191403", "reactome_id": "R-ALL-191403", "label": "MVA5PP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57557", "formula": "C6H10O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-191362", "reactome_id": "R-ALL-191362", "label": "IPPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:128769", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "diphosphomevalonate decarboxylase activity of MVD dimer [cytosol]", "activity_id": "191297", "ec_numbers": [ "4.1.1.33" ], "accessions": [] } ], "citations": [ { "id": "PMID:8626466", "title": "Molecular cloning and expression of the cDNAs encoding human and yeast mevalonate pyrophosphate decarboxylase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8626466/" } ], "x": 2938.666666666667, "y": 2050.9, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191422", "label": "Mevalonate-5-phosphate is further phosphorylated", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16342" ], "source_url": "https://reactome.org/content/detail/R-HSA-191422", "summary": "Mevalonate-5-phosphate is further phosphorylated is a Reactome release 97 curated reaction event. The source record connects MVA5P, ATP to MVA5PP, ADP in cytosol and cross-references RHEA:16342.", "equation": "MVA5P + ATP → MVA5PP + ADP", "inputs": [ { "node_id": "R-ALL-191296", "reactome_id": "R-ALL-191296", "label": "MVA5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58146", "formula": "C6H10O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-191403", "reactome_id": "R-ALL-191403", "label": "MVA5PP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57557", "formula": "C6H10O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphomevalonate kinase activity of PMVK [cytosol]", "activity_id": "191287", "ec_numbers": [ "2.7.4.2" ], "accessions": [ "Q15126" ] } ], "citations": [ { "id": "PMID:16519518", "title": "Phosphomevalonate kinase: functional investigation of the recombinant human enzyme", "journal": "Biochemistry", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16519518/" }, { "id": "PMID:14729858", "title": "Phosphomevalonate kinase is a cytosolic protein in humans", "journal": "J Lipid Res", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14729858/" } ], "x": 2660, "y": 2206.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191971", "label": "THCA is translocated from the mitochondrial matrix to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-191971", "summary": "THCA is translocated from the mitochondrial matrix to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects THCA to THCA in mitochondrial inner membrane.", "equation": "THCA → THCA", "inputs": [ { "node_id": "R-ALL-192161", "reactome_id": "R-ALL-192161", "label": "THCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18402", "formula": "C27H46O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192013", "reactome_id": "R-ALL-192013", "label": "THCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18402", "formula": "C27H46O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 4730, "y": 1836.5378787878788, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-191972", "label": "27-hydroxycholesterol is 7alpha-hydroxylated", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-191972", "summary": "27-hydroxycholesterol is 7alpha-hydroxylated is a Reactome release 97 curated reaction event. The source record connects 27HCHOL, NADPH, H+ to Cholest-5-ene-3beta,7alpha,27-triol, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "27HCHOL + NADPH + H+ + O2 → Cholest-5-ene-3beta,7alpha,27-triol + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-176617", "reactome_id": "R-ALL-176617", "label": "27HCHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17703", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192194", "reactome_id": "R-ALL-192194", "label": "Cholest-5-ene-3beta,7alpha,27-triol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18431", "formula": "C27H46O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxysterol 7-alpha-hydroxylase activity of Cytochrome P450 (CYP7B1 based) [endoplasmic reticulum membrane]", "activity_id": "192095", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10588945", "title": "Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1", "journal": "J Lipid Res", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10588945/" } ], "x": 4730, "y": 1642.0378787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191983", "label": "Cholesterol is hydroxylated to 25-hydroxycholesterol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:46133" ], "source_url": "https://reactome.org/content/detail/R-HSA-191983", "summary": "Cholesterol is hydroxylated to 25-hydroxycholesterol is a Reactome release 97 curated reaction event. The source record connects CHOL, NADPH, H+ to H2O, NADP+, 25OH-CHOL in cytosol · endoplasmic reticulum membrane and cross-references RHEA:46133.", "equation": "CHOL + NADPH + H+ + O2 → H2O + NADP+ + 25OH-CHOL", "inputs": [ { "node_id": "R-ALL-176478", "reactome_id": "R-ALL-176478", "label": "CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-192148", "reactome_id": "R-ALL-192148", "label": "25OH-CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:42977", "formula": "C27H46O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid hydroxylase activity of CH25H (Fe2+ cofactor) [endoplasmic reticulum membrane]", "activity_id": "192119", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9852097", "title": "cDNA cloning of mouse and human cholesterol 25-hydroxylases, polytopic membrane proteins that synthesize a potent oxysterol regulator of lipid metabolism", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9852097/" } ], "x": 5008.666666666667, "y": 1547.7348484848485, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-191999", "label": "CYP27A1 27-hydroxylates 5bCHOL3a,7a,12a-triol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-191999", "summary": "CYP27A1 27-hydroxylates 5bCHOL3a,7a,12a-triol is a Reactome release 97 curated reaction event. The source record connects 5bCHOL3a,7a,12a-triol, NADPH, H+ to 5bCHOL3a,7a,12a,27-tetrol, H2O, NADP+ in mitochondrial matrix.", "equation": "5bCHOL3a,7a,12a-triol + NADPH + H+ + O2 → 5bCHOL3a,7a,12a,27-tetrol + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-192078", "reactome_id": "R-ALL-192078", "label": "5bCHOL3a,7a,12a-triol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16496", "formula": "C27H48O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192201", "reactome_id": "R-ALL-192201", "label": "5bCHOL3a,7a,12a,27-tetrol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17278", "formula": "C27H48O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:2019602", "title": "Mutations in the bile acid biosynthetic enzyme sterol 27-hydroxylase underlie cerebrotendinous xanthomatosis", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2019602/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 5008.666666666667, "y": 1642.0378787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192010", "label": "5beta-cholestan-3alpha, 7alpha, 12alpha-triol is translocated from the cytosol to the mitochondrial matrix", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-192010", "summary": "5beta-cholestan-3alpha, 7alpha, 12alpha-triol is translocated from the cytosol to the mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 5beta-cholestan-3alpha,7alpha,12alpha-triol to 5bCHOL3a,7a,12a-triol in mitochondrial inner membrane.", "equation": "5beta-cholestan-3alpha,7alpha,12alpha-triol → 5bCHOL3a,7a,12a-triol", "inputs": [ { "node_id": "R-ALL-192087", "reactome_id": "R-ALL-192087", "label": "5beta-cholestan-3alpha,7alpha,12alpha-triol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16496", "formula": "C27H48O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192078", "reactome_id": "R-ALL-192078", "label": "5bCHOL3a,7a,12a-triol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16496", "formula": "C27H48O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol transmembrane transporter activity of unknown START protein [mitochondrial inner membrane]", "activity_id": "9948409", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 4451.333333333333, "y": 1724.5530303030303, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-192033", "label": "4-cholesten-7alpha-ol-3-one is reduced to 5beta-cholestan-7alpha-ol-3-one", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:46640", "RHEA:46641" ], "source_url": "https://reactome.org/content/detail/R-HSA-192033", "summary": "4-cholesten-7alpha-ol-3-one is reduced to 5beta-cholestan-7alpha-ol-3-one is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 4CHOL7aOLONE, NADPH, H+ to 5beta-cholestan-7alpha-ol-3-one, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:46640, RHEA:46641.", "equation": "4CHOL7aOLONE + NADPH + H+ → 5beta-cholestan-7alpha-ol-3-one + NADP+", "inputs": [ { "node_id": "R-ALL-192136", "reactome_id": "R-ALL-192136", "label": "4CHOL7aOLONE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17899", "formula": "C27H44O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-191980", "reactome_id": "R-ALL-191980", "label": "5beta-cholestan-7alpha-ol-3-one", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:2290", "formula": "C27H46O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of AKR1D1 [cytosol]", "activity_id": "192006", "ec_numbers": [ "1.1.1.2" ], "accessions": [ "P51857" ] } ], "citations": [ { "id": "PMID:7508385", "title": "Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7508385/" } ], "x": 4451.333333333333, "y": 1553.628787878788, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-192036", "label": "5Beta-cholesten-7alpha, 12alpha-diol-3-one is reduced to 5beta-cholestan-3alpha, 7alpha, 12alpha-triol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-192036", "summary": "5Beta-cholesten-7alpha, 12alpha-diol-3-one is reduced to 5beta-cholestan-3alpha, 7alpha, 12alpha-triol is a Reactome release 97 curated reaction event. The source record connects 5beta-cholesten-7alpha, 12alpha-diol-3-one, NADPH, H+ to 5beta-cholestan-3alpha,7alpha,12alpha-triol, NADP+ in cytosol.", "equation": "5beta-cholesten-7alpha, 12alpha-diol-3-one + NADPH + H+ → 5beta-cholestan-3alpha,7alpha,12alpha-triol + NADP+", "inputs": [ { "node_id": "R-ALL-192064", "reactome_id": "R-ALL-192064", "label": "5beta-cholesten-7alpha, 12alpha-diol-3-one", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2288", "formula": "C27H46O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192087", "reactome_id": "R-ALL-192087", "label": "5beta-cholestan-3alpha,7alpha,12alpha-triol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16496", "formula": "C27H48O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of Aldo-keto reductase family [cytosol]", "activity_id": "192098", "ec_numbers": [ "1.1.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11158055", "title": "Human types 1 and 3 3 alpha-hydroxysteroid dehydrogenases: differential lability and tissue distribution", "journal": "J Clin Endocrinol Metab", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11158055/" } ], "x": 4451.333333333333, "y": 1689.189393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192042", "label": "5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-192042", "summary": "5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al is a Reactome release 97 curated reaction event. The source record connects 5bCHOL3a,7a,12a,27-tetrol, NADP+ to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al, NADPH, H+ in mitochondrial matrix.", "equation": "5bCHOL3a,7a,12a,27-tetrol + NADP+ → 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al + NADPH + H+", "inputs": [ { "node_id": "R-ALL-192201", "reactome_id": "R-ALL-192201", "label": "5bCHOL3a,7a,12a,27-tetrol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17278", "formula": "C27H48O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-191968", "reactome_id": "R-ALL-191968", "label": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16466", "formula": "C27H46O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 4730, "y": 1689.189393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192051", "label": "CYP7A1 7-hydroxylates CHOL", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:21813" ], "source_url": "https://reactome.org/content/detail/R-HSA-192051", "summary": "CYP7A1 7-hydroxylates CHOL is a Reactome release 97 curated reaction event. The source record connects CHOL, NADPH, H+ to H2O, NADP+, 7 alpha-hydroxycholesterol in cytosol · endoplasmic reticulum membrane and cross-references RHEA:21813.", "equation": "CHOL + NADPH + H+ + O2 → H2O + NADP+ + 7 alpha-hydroxycholesterol", "inputs": [ { "node_id": "R-ALL-193384", "reactome_id": "R-ALL-193384", "label": "CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-192114", "reactome_id": "R-ALL-192114", "label": "7 alpha-hydroxycholesterol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17500", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cholesterol 7-alpha-monooxygenase activity of CYP7A1 [endoplasmic reticulum membrane]", "activity_id": "192120", "ec_numbers": [ "1.14.14.23" ], "accessions": [ "P22680" ] } ], "citations": [ { "id": "PMID:2384150", "title": "Molecular cloning and sequence analysis of cDNA encoding human cholesterol 7 alpha-hydroxylase", "journal": "FEBS Lett", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2384150/" } ], "x": 4730, "y": 1571.310606060606, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192054", "label": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate (THCA)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-192054", "summary": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoate (THCA) is a Reactome release 97 curated reaction event. The source record connects 3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al, NADPH, H+ to THCA, NADP+, H2O in mitochondrial matrix.", "equation": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al + NADPH + H+ + O2 → THCA + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-191968", "reactome_id": "R-ALL-191968", "label": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholestan-27-al", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16466", "formula": "C27H46O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192161", "reactome_id": "R-ALL-192161", "label": "THCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18402", "formula": "C27H46O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 4451.333333333333, "y": 1647.9318181818182, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192056", "label": "Isomerization of 25(R) THCA-CoA to 25(S) THCA-CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:40456" ], "source_url": "https://reactome.org/content/detail/R-HSA-192056", "summary": "Isomerization of 25(R) THCA-CoA to 25(S) THCA-CoA is a Reactome release 97 curated reaction event. The source record connects 25(R) THCA-CoA to 25(S) THCA-CoA in peroxisomal matrix and cross-references RHEA:40456.", "equation": "25(R) THCA-CoA → 25(S) THCA-CoA", "inputs": [ { "node_id": "R-ALL-192308", "reactome_id": "R-ALL-192308", "label": "25(R) THCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58677", "formula": "C48H76N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192316", "reactome_id": "R-ALL-192316", "label": "25(S) THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77251", "formula": "C48H76N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "racemase and epimerase activity of AMACR [peroxisomal matrix]", "activity_id": "191973", "ec_numbers": [ "5.1" ], "accessions": [ "Q9UHK6" ] } ], "citations": [ { "id": "PMID:7649182", "title": "Purification and characterization of an alpha-methylacyl-CoA racemase from human liver", "journal": "Eur J Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7649182/" }, { "id": "PMID:11060344", "title": "Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA racemase in humans", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11060344/" }, { "id": "PMID:10655068", "title": "Mutations in the gene encoding peroxisomal alpha-methylacyl-CoA racemase cause adult-onset sensory motor neuropathy", "journal": "Nat Genet", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10655068/" } ], "x": 4730, "y": 1565.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192061", "label": "CYP46A1 24-hydroxylates CHOL", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:22717" ], "source_url": "https://reactome.org/content/detail/R-HSA-192061", "summary": "CYP46A1 24-hydroxylates CHOL is a Reactome release 97 curated reaction event. The source record connects CHOL, NADPH, H+ to 24OH-CHOL, NADP+, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:22717.", "equation": "CHOL + NADPH + H+ + O2 → 24OH-CHOL + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-193384", "reactome_id": "R-ALL-193384", "label": "CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192060", "reactome_id": "R-ALL-192060", "label": "24OH-CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:34310", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cholesterol 24-hydroxylase activity of CYP46A1 [endoplasmic reticulum membrane]", "activity_id": "192143", "ec_numbers": [ "1.14.14.25" ], "accessions": [ "Q9Y6A2" ] } ], "citations": [ { "id": "PMID:14640697", "title": "Broad substrate specificity of human cytochrome P450 46A1 which initiates cholesterol degradation in the brain", "journal": "Biochemistry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14640697/" }, { "id": "PMID:10377398", "title": "cDNA cloning of cholesterol 24-hydroxylase, a mediator of cholesterol homeostasis in the brain", "journal": "Proc Natl Acad Sci U S A", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10377398/" } ], "x": 5008.666666666667, "y": 1571.310606060606, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192065", "label": "CYP7B1 7-hydroxylates 25OH-CHOL", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:24309" ], "source_url": "https://reactome.org/content/detail/R-HSA-192065", "summary": "CYP7B1 7-hydroxylates 25OH-CHOL is a Reactome release 97 curated reaction event. The source record connects 25OH-CHOL, NADPH, H+ to CHOL3b,7a,25TRIOL, H2O, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:24309.", "equation": "25OH-CHOL + NADPH + H+ + O2 → CHOL3b,7a,25TRIOL + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-192148", "reactome_id": "R-ALL-192148", "label": "25OH-CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:42977", "formula": "C27H46O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192184", "reactome_id": "R-ALL-192184", "label": "CHOL3b,7a,25TRIOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:37623", "formula": "C27H46O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxysterol 7-alpha-hydroxylase activity of Cytochrome P450 (CYP7B1 based) [endoplasmic reticulum membrane]", "activity_id": "192095", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10588945", "title": "Structure and functions of human oxysterol 7alpha-hydroxylase cDNAs and gene CYP7B1", "journal": "J Lipid Res", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10588945/" } ], "x": 4451.333333333333, "y": 1577.2045454545455, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192067", "label": "4-cholesten-7alpha, 12alpha-diol-3-one is reduced to 5beta-cholesten-7alpha, 12alpha-diol-3-one", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:46632", "RHEA:46633" ], "source_url": "https://reactome.org/content/detail/R-HSA-192067", "summary": "4-cholesten-7alpha, 12alpha-diol-3-one is reduced to 5beta-cholesten-7alpha, 12alpha-diol-3-one is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 4CHOL7a,12aDONE, NADPH, H+ to 5beta-cholesten-7alpha, 12alpha-diol-3-one, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:46632, RHEA:46633.", "equation": "4CHOL7a,12aDONE + NADPH + H+ → 5beta-cholesten-7alpha, 12alpha-diol-3-one + NADP+", "inputs": [ { "node_id": "R-ALL-192195", "reactome_id": "R-ALL-192195", "label": "4CHOL7a,12aDONE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28477", "formula": "C27H44O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192064", "reactome_id": "R-ALL-192064", "label": "5beta-cholesten-7alpha, 12alpha-diol-3-one", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:2288", "formula": "C27H46O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of AKR1D1 [cytosol]", "activity_id": "192006", "ec_numbers": [ "1.1.1.2" ], "accessions": [ "P51857" ] } ], "citations": [ { "id": "PMID:7508385", "title": "Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7508385/" } ], "x": 4730, "y": 1553.628787878788, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-192097", "label": "7alpha-hydroxycholesterol is oxidized and isomerized to 4-cholesten-7alpha-ol-3-one", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:11896" ], "source_url": "https://reactome.org/content/detail/R-HSA-192097", "summary": "7alpha-hydroxycholesterol is oxidized and isomerized to 4-cholesten-7alpha-ol-3-one is a Reactome release 97 curated reaction event. The source record connects 7 alpha-hydroxycholesterol, NAD+ to 4CHOL7aOLONE, NADH, H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:11896.", "equation": "7 alpha-hydroxycholesterol + NAD+ → 4CHOL7aOLONE + NADH + H+", "inputs": [ { "node_id": "R-ALL-192114", "reactome_id": "R-ALL-192114", "label": "7 alpha-hydroxycholesterol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17500", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192136", "reactome_id": "R-ALL-192136", "label": "4CHOL7aOLONE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17899", "formula": "C27H44O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of HSD3B7 [endoplasmic reticulum membrane]", "activity_id": "192106", "ec_numbers": [ "1.1.1" ], "accessions": [ "Q9H2F3" ] } ], "citations": [ { "id": "PMID:12679481", "title": "Molecular genetics of 3beta-hydroxy-Delta5-C27-steroid oxidoreductase deficiency in 16 patients with loss of bile acid synthesis and liver disease", "journal": "J Clin Endocrinol Metab", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12679481/" }, { "id": "PMID:11067870", "title": "The bile acid synthetic gene 3beta-hydroxy-Delta(5)-C(27)-steroid oxidoreductase is mutated in progressive intrahepatic cholestasis", "journal": "J Clin Invest", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11067870/" } ], "x": 4451.333333333333, "y": 1600.780303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192123", "label": "CYP27A1 27-hydroxylates CHOL", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "bile-acids", "biological-oxidations", "phase-one", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:43837" ], "source_url": "https://reactome.org/content/detail/R-HSA-192123", "summary": "CYP27A1 27-hydroxylates CHOL is a Reactome release 97 curated reaction event. The source record connects CHOL, NADPH, H+ to 27OH-CHOL, H2O, NADP+ in mitochondrial matrix and cross-references RHEA:43837.", "equation": "CHOL + NADPH + H+ + O2 → 27OH-CHOL + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-193840", "reactome_id": "R-ALL-193840", "label": "CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193846", "reactome_id": "R-ALL-193846", "label": "27OH-CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17703", "formula": "C27H46O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" } ], "x": 6521.333333333333, "y": 3476.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192137", "label": "THCA is conjugated with Coenzyme A (SLC27A5 BACS)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:22977" ], "source_url": "https://reactome.org/content/detail/R-HSA-192137", "summary": "THCA is conjugated with Coenzyme A (SLC27A5 BACS) is a Reactome release 97 curated reaction event. The source record connects THCA, ATP, CoA-SH to 25(R) THCA-CoA, AMP, PPi in cytosol · endoplasmic reticulum membrane and cross-references RHEA:22977.", "equation": "THCA + ATP + CoA-SH → 25(R) THCA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-192013", "reactome_id": "R-ALL-192013", "label": "THCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18402", "formula": "C27H46O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192096", "reactome_id": "R-ALL-192096", "label": "25(R) THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58677", "formula": "C48H76N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of SLC27A5 [endoplasmic reticulum membrane]", "activity_id": "192026", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "Q9Y2P5" ] } ], "citations": [ { "id": "PMID:10479480", "title": "Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization of a second enzymatically active protein", "journal": "Mol Genet Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10479480/" }, { "id": "PMID:10749848", "title": "The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10749848/" }, { "id": "PMID:11980911", "title": "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980911/" } ], "x": 4730, "y": 1594.8863636363637, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192157", "label": "CYP8B1 12-hydroxylates 4CHOL7aOLONE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:46753" ], "source_url": "https://reactome.org/content/detail/R-HSA-192157", "summary": "CYP8B1 12-hydroxylates 4CHOL7aOLONE is a Reactome release 97 curated reaction event. The source record connects 4CHOL7aOLONE, NADPH, H+ to 4CHOL7a,12aDONE, NADP+, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:46753.", "equation": "4CHOL7aOLONE + NADPH + H+ + O2 → 4CHOL7a,12aDONE + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-192136", "reactome_id": "R-ALL-192136", "label": "4CHOL7aOLONE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17899", "formula": "C27H44O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192195", "reactome_id": "R-ALL-192195", "label": "4CHOL7a,12aDONE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28477", "formula": "C27H44O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sterol 12-alpha-hydroxylase activity of CYP8B1 [endoplasmic reticulum membrane]", "activity_id": "192125", "ec_numbers": [ "1.14.14.139" ], "accessions": [ "Q9UNU6" ] } ], "citations": [ { "id": "PMID:10051404", "title": "Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10051404/" }, { "id": "PMID:1400444", "title": "Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase", "journal": "J. Biol. Chem.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1400444/" } ], "x": 4730, "y": 1577.2045454545455, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192160", "label": "5beta-cholestan-7alpha-ol-3-one is reduced to 5beta-cholestan-3alpha, 7alpha-diol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-192160", "summary": "5beta-cholestan-7alpha-ol-3-one is reduced to 5beta-cholestan-3alpha, 7alpha-diol is a Reactome release 97 curated reaction event. The source record connects 5beta-cholestan-7alpha-ol-3-one, NADPH, H+ to 5beta-cholestan-3alpha,7alpha-diol, NADP+ in cytosol.", "equation": "5beta-cholestan-7alpha-ol-3-one + NADPH + H+ → 5beta-cholestan-3alpha,7alpha-diol + NADP+", "inputs": [ { "node_id": "R-ALL-191980", "reactome_id": "R-ALL-191980", "label": "5beta-cholestan-7alpha-ol-3-one", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2290", "formula": "C27H46O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192030", "reactome_id": "R-ALL-192030", "label": "5beta-cholestan-3alpha,7alpha-diol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28047", "formula": "C27H48O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of Aldo-keto reductase family [cytosol]", "activity_id": "192098", "ec_numbers": [ "1.1.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11158055", "title": "Human types 1 and 3 3 alpha-hydroxysteroid dehydrogenases: differential lability and tissue distribution", "journal": "J Clin Endocrinol Metab", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11158055/" } ], "x": 5008.666666666667, "y": 1689.189393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192178", "label": "CYP39A1 7-hydroxylates 24OH-CHOL", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:46125" ], "source_url": "https://reactome.org/content/detail/R-HSA-192178", "summary": "CYP39A1 7-hydroxylates 24OH-CHOL is a Reactome release 97 curated reaction event. The source record connects 24OH-CHOL, O2, hydrogen donor to CHOL7a,24(S)DIOL, H2O, hydrogen acceptor in cytosol · endoplasmic reticulum membrane and cross-references RHEA:46125.", "equation": "24OH-CHOL + O2 + hydrogen donor → CHOL7a,24(S)DIOL + H2O + hydrogen acceptor + H+", "inputs": [ { "node_id": "R-ALL-192060", "reactome_id": "R-ALL-192060", "label": "24OH-CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:34310", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1131953", "reactome_id": "R-ALL-1131953", "label": "hydrogen donor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17499", "formula": "H2A", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192189", "reactome_id": "R-ALL-192189", "label": "CHOL7a,24(S)DIOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:37640", "formula": "C27H46O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1130886", "reactome_id": "R-ALL-1130886", "label": "hydrogen acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13193", "formula": "A", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxysterol 7-alpha-hydroxylase activity of CYP39A1 [endoplasmic reticulum membrane]", "activity_id": "192129", "ec_numbers": null, "accessions": [ "Q9NYL5" ] } ], "citations": [ { "id": "PMID:10748047", "title": "Expression cloning of an oxysterol 7alpha-hydroxylase selective for 24-hydroxycholesterol", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10748047/" } ], "x": 5008.666666666667, "y": 1577.2045454545455, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192312", "label": "Choloyl CoA reacts with glycine or taurine to form glycocholate or taurocholate", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:47101", "RHEA:14002" ], "source_url": "https://reactome.org/content/detail/R-HSA-192312", "summary": "Choloyl CoA reacts with glycine or taurine to form glycocholate or taurocholate is a Reactome release 97 curated reaction event. The source record connects choloyl-CoA, Gly, TAU to cholate bile salts, CoA-SH, H+ in peroxisomal matrix and cross-references RHEA:47101, RHEA:14002.", "equation": "choloyl-CoA + Gly, TAU → cholate bile salts + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-192356", "reactome_id": "R-ALL-192356", "label": "choloyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57373", "formula": "C45H70N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193463", "reactome_id": "R-ALL-193463", "label": "Gly, TAU", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193404", "reactome_id": "R-ALL-193404", "label": "cholate bile salts", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyltransferase activity, transferring groups other than amino-acyl groups of BAAT [peroxisomal matrix]", "activity_id": "193371", "ec_numbers": [ "2.3.1" ], "accessions": [ "Q14032" ] } ], "citations": [ { "id": "PMID:8034703", "title": "Glycine and taurine conjugation of bile acids by a single enzyme. Molecular cloning and expression of human liver bile acid CoA:amino acid N-acyltransferase.", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8034703/" } ], "x": 4451.333333333333, "y": 1541.840909090909, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192315", "label": "Bile salts are translocated from the peroxisomal matrix to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal membrane", "compartment_ids": [ "GO:0005778" ], "rhea_ids": [ "RHEA:71855", "RHEA:71851", "RHEA:71859", "RHEA:71703" ], "source_url": "https://reactome.org/content/detail/R-HSA-192315", "summary": "Bile salts are translocated from the peroxisomal matrix to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects bile salts to bile salts in peroxisomal membrane and cross-references RHEA:71855, RHEA:71851, RHEA:71859, RHEA:71703.", "equation": "bile salts → bile salts", "inputs": [ { "node_id": "R-ALL-193448", "reactome_id": "R-ALL-193448", "label": "bile salts", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193416", "reactome_id": "R-ALL-193416", "label": "bile salts", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 4730, "y": 1547.7348484848485, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-192325", "label": "25(R) THCA-CoA is translocated from the cytosol to the peroxisome", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal membrane", "compartment_ids": [ "GO:0005778" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-192325", "summary": "25(R) THCA-CoA is translocated from the cytosol to the peroxisome is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 25(R) THCA-CoA, ATP, H2O to 25(R) THCA-CoA, ADP, H+ in peroxisomal membrane.", "equation": "25(R) THCA-CoA + ATP + H2O → 25(R) THCA-CoA + ADP + H+ + Pi", "inputs": [ { "node_id": "R-ALL-192096", "reactome_id": "R-ALL-192096", "label": "25(R) THCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58677", "formula": "C48H76N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192308", "reactome_id": "R-ALL-192308", "label": "25(R) THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58677", "formula": "C48H76N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ATPase-coupled transmembrane transporter activity of ABCD3 dimer [peroxisomal membrane]", "activity_id": "9987431", "ec_numbers": [ "7.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:25168382", "title": "A novel bile acid biosynthesis defect due to a deficiency of peroxisomal ABCD3", "journal": "Hum Mol Genet", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25168382/" }, { "id": "PMID:17609205", "title": "Live cell FRET microscopy: homo- and heterodimerization of two human peroxisomal ABC transporters, the adrenoleukodystrophy protein (ALDP, ABCD1) and PMP70 (ABCD3)", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17609205/" }, { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" }, { "id": "PMID:12966071", "title": "ATP-dependent transport of bile acid intermediates across rat liver peroxisomal membranes", "journal": "J Biochem (Tokyo)", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12966071/" } ], "x": 4730, "y": 1647.9318181818182, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-192331", "label": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA (THCA-CoA) is hydrated to (24R, 25R) 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:18935" ], "source_url": "https://reactome.org/content/detail/R-HSA-192331", "summary": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA (THCA-CoA) is hydrated to (24R, 25R) 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects THCA-CoA, H2O to (24R, 25R) 3a,7a,12a,24-tetrahydroxy-5b-cholestanoyl-CoA in peroxisomal matrix and cross-references RHEA:18935.", "equation": "THCA-CoA + H2O → (24R, 25R) 3a,7a,12a,24-tetrahydroxy-5b-cholestanoyl-CoA", "inputs": [ { "node_id": "R-ALL-192342", "reactome_id": "R-ALL-192342", "label": "THCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59879", "formula": "C48H74N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193422", "reactome_id": "R-ALL-193422", "label": "(24R, 25R) 3a,7a,12a,24-tetrahydroxy-5b-cholestanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59807", "formula": "C48H76N7O21P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "192350", "ec_numbers": [ "4.2.1.107" ], "accessions": [] } ], "citations": [ { "id": "PMID:8902629", "title": "Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase", "journal": "J Biochem (Tokyo)", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8902629/" } ], "x": 5008.666666666667, "y": 1624.3560606060605, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192335", "label": "25(S) THCA-CoA is dehydrogenated to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA (THCA-CoA)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:46729" ], "source_url": "https://reactome.org/content/detail/R-HSA-192335", "summary": "25(S) THCA-CoA is dehydrogenated to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA (THCA-CoA) is a Reactome release 97 curated reaction event. The source record connects 25(S) THCA-CoA, O2 to THCA-CoA, H2O2 in peroxisomal matrix and cross-references RHEA:46729.", "equation": "25(S) THCA-CoA + O2 → THCA-CoA + H2O2", "inputs": [ { "node_id": "R-ALL-192316", "reactome_id": "R-ALL-192316", "label": "25(S) THCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77251", "formula": "C48H76N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192342", "reactome_id": "R-ALL-192342", "label": "THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59879", "formula": "C48H74N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA oxidase activity of ACOX2:FAD [peroxisomal matrix]", "activity_id": "192336", "ec_numbers": [ "1.3.3.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:8943006", "title": "Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8943006/" }, { "id": "PMID:8387517", "title": "The CoA esters of 2-methyl-branched chain fatty acids and of the bile acid intermediates di- and trihydroxycoprostanic acids are oxidized by one single peroxisomal branched chain acyl-CoA oxidase in human liver and kidney", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8387517/" } ], "x": 4730, "y": 1624.3560606060605, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-192341", "label": "Thiolysis of 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-one-CoA yields choloyl-CoA (3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-one-CoA) and propionyl CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:16867" ], "source_url": "https://reactome.org/content/detail/R-HSA-192341", "summary": "Thiolysis of 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-one-CoA yields choloyl-CoA (3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-one-CoA) and propionyl CoA is a Reactome release 97 curated reaction event. The source record connects 3a,7a,12a-trihydroxy-5b-cholest-24-one-CoA, CoA-SH to choloyl-CoA, propionyl CoA in peroxisomal matrix and cross-references RHEA:16867.", "equation": "3a,7a,12a-trihydroxy-5b-cholest-24-one-CoA + CoA-SH → choloyl-CoA + propionyl CoA", "inputs": [ { "node_id": "R-ALL-192339", "reactome_id": "R-ALL-192339", "label": "3a,7a,12a-trihydroxy-5b-cholest-24-one-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58507", "formula": "C48H74N7O21P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192356", "reactome_id": "R-ALL-192356", "label": "choloyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57373", "formula": "C45H70N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192314", "reactome_id": "R-ALL-192314", "label": "propionyl CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57392", "formula": "C24H36N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "propanoyl-CoA C-acyltransferase activity of SCP2-1 [peroxisomal matrix]", "activity_id": "192310", "ec_numbers": [ "2.3.1.176" ], "accessions": [ "P22307" ] } ], "citations": [ { "id": "PMID:1703300", "title": "Cloning and expression of a cDNA encoding human sterol carrier protein 2", "journal": "Proc Natl Acad Sci U S A", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1703300/" }, { "id": "PMID:10706581", "title": "Peroxisomal fatty acid oxidation disorders and 58 kDa sterol carrier protein X (SCPx). Activity measurements in liver and fibroblasts using a newly developed method.", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10706581/" } ], "x": 4451.333333333333, "y": 1565.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193052", "label": "Pregn-5-ene-3,20-dione isomerizes to progesterone", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:43929" ], "source_url": "https://reactome.org/content/detail/R-HSA-193052", "summary": "Pregn-5-ene-3,20-dione isomerizes to progesterone is a Reactome release 97 curated reaction event. The source record connects pregn-5-ene-3,20-dione to P4 in cytosol · endoplasmic reticulum membrane and cross-references RHEA:43929.", "equation": "pregn-5-ene-3,20-dione → P4", "inputs": [ { "node_id": "R-ALL-196344", "reactome_id": "R-ALL-196344", "label": "pregn-5-ene-3,20-dione", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63837", "formula": "C21H30O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193055", "reactome_id": "R-ALL-193055", "label": "P4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17026", "formula": "C21H30O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid Delta-isomerase activity of HSD3B1 dimer, HSD3B2 dimer [endoplasmic reticulum membrane]", "activity_id": "193169", "ec_numbers": [ "5.3.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2243100", "title": "Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2243100/" }, { "id": "PMID:2770297", "title": "Human placental 3 beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5----4-ene-isomerase: purification from mitochondria and kinetic profiles, biophysical characterization of the purified mitochondrial and microsomal enzymes", "journal": "J Steroid Biochem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2770297/" }, { "id": "PMID:1944309", "title": "Structure and expression of a new complementary DNA encoding the almost exclusive 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase in human adrenals and gonads", "journal": "Mol Endocrinol", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1944309/" }, { "id": "PMID:12832414", "title": "Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12832414/" } ], "x": 4451.333333333333, "y": 1630.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193054", "label": "Oxidation of cholesterol to 22beta-hydroxycholesterol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193054", "summary": "Oxidation of cholesterol to 22beta-hydroxycholesterol is a Reactome release 97 curated reaction event. The source record connects CHOL, NADPH, O2 to 22b-HCHOL, H2O, NADP+ in mitochondrial matrix · mitochondrial inner membrane.", "equation": "CHOL + NADPH + O2 + H+ → 22b-HCHOL + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-193156", "reactome_id": "R-ALL-193156", "label": "CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193019", "reactome_id": "R-ALL-193019", "label": "22b-HCHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:1301", "formula": "C27H46O2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cholesterol monooxygenase (side-chain-cleaving) activity of CYP11A1:FDXR:FDX1,FDX1L (red.) [mitochondrial matrix]", "activity_id": "193154", "ec_numbers": [ "1.14.15.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:9578606", "title": "Expression of catalytically active human cytochrome p450scc in Escherichia coli and mutagenesis of isoleucine-462", "journal": "Arch Biochem Biophys", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9578606/" }, { "id": "PMID:3024157", "title": "Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3024157/" } ], "x": 5008.666666666667, "y": 1647.9318181818182, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193060", "label": "CYP19A1 hydroxylates ANDST to E1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:38196" ], "source_url": "https://reactome.org/content/detail/R-HSA-193060", "summary": "CYP19A1 hydroxylates ANDST to E1 is a Reactome release 97 curated reaction event. The source record connects ANDST, NADPH, H+ to H+, E1, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:38196.", "equation": "ANDST + 3 NADPH + 3 H+ + 3 O2 → 4 H+ + E1 + 3 NADP+ + 4 H2O + HCOOH", "inputs": [ { "node_id": "R-ALL-193079", "reactome_id": "R-ALL-193079", "label": "ANDST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16422", "formula": "C19H26O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193066", "reactome_id": "R-ALL-193066", "label": "E1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17263", "formula": "C18H22O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid hydroxylase activity of CYP19A1 [endoplasmic reticulum membrane]", "activity_id": "193170", "ec_numbers": null, "accessions": [ "P11511" ] } ], "citations": [ { "id": "PMID:8076586", "title": "Aromatase cytochrome P450, the enzyme responsible for estrogen biosynthesis", "journal": "Endocr Rev", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8076586/" }, { "id": "PMID:2171939", "title": "Structural and functional characterization of human aromatase P-450 gene", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2171939/" } ], "x": 5008.666666666667, "y": 1565.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193064", "label": "HSD17B3-like proteins reducde ANDST to TEST", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:14983" ], "source_url": "https://reactome.org/content/detail/R-HSA-193064", "summary": "HSD17B3-like proteins reducde ANDST to TEST is a Reactome release 97 curated reaction event. The source record connects ANDST, NADPH, H+ to TEST, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:14983.", "equation": "ANDST + NADPH + H+ → TEST + NADP+", "inputs": [ { "node_id": "R-ALL-193079", "reactome_id": "R-ALL-193079", "label": "ANDST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16422", "formula": "C19H26O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193057", "reactome_id": "R-ALL-193057", "label": "TEST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17347", "formula": "C19H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "testosterone dehydrogenase (NADP+) activity of HSD17B3-like Proteins [endoplasmic reticulum membrane]", "activity_id": "193120", "ec_numbers": [ "1.1.1.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:2197970", "title": "Purification, cloning, complementary DNA structure, and predicted amino acid sequence of human estradiol 17 beta-dehydrogenase", "journal": "Ann N Y Acad Sci", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2197970/" }, { "id": "PMID:8547185", "title": "Characteristics of human types 1, 2 and 3 17 beta-hydroxysteroid dehydrogenase activities: oxidation/reduction and inhibition", "journal": "J Steroid Biochem Mol Biol", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8547185/" }, { "id": "PMID:8075637", "title": "Male pseudohermaphroditism caused by mutations of testicular 17 beta-hydroxysteroid dehydrogenase 3", "journal": "Nat Genet", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8075637/" }, { "id": "PMID:9536209", "title": "A critical review of the origin and control of adrenal androgens", "journal": "Baillieres Clin Obstet Gynaecol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9536209/" } ], "x": 4730, "y": 1600.780303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193065", "label": "Oxidation of 22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193065", "summary": "Oxidation of 22beta-hydroxycholesterol to 20alpha,22beta-hydroxycholesterol is a Reactome release 97 curated reaction event. The source record connects 22b-HCHOL, NADPH, O2 to 20a,22b-DHCHOL, H2O, NADP+ in mitochondrial matrix · mitochondrial inner membrane.", "equation": "22b-HCHOL + NADPH + O2 + H+ → 20a,22b-DHCHOL + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-193019", "reactome_id": "R-ALL-193019", "label": "22b-HCHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:1301", "formula": "C27H46O2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193086", "reactome_id": "R-ALL-193086", "label": "20a,22b-DHCHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:1294", "formula": "C27H46O3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cholesterol monooxygenase (side-chain-cleaving) activity of CYP11A1:FDXR:FDX1,FDX1L (red.) [mitochondrial matrix]", "activity_id": "193154", "ec_numbers": [ "1.14.15.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:3024157", "title": "Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3024157/" }, { "id": "PMID:9578606", "title": "Expression of catalytically active human cytochrome p450scc in Escherichia coli and mutagenesis of isoleucine-462", "journal": "Arch Biochem Biophys", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9578606/" } ], "x": 4451.333333333333, "y": 1653.8257575757575, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193068", "label": "CYP17A1 17-hydroxylates PREG", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:50237" ], "source_url": "https://reactome.org/content/detail/R-HSA-193068", "summary": "CYP17A1 17-hydroxylates PREG is a Reactome release 97 curated reaction event. The source record connects PREG, O2, NADPH to 17aHPREG, NADP+, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:50237.", "equation": "PREG + O2 + NADPH + H+ → 17aHPREG + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-193103", "reactome_id": "R-ALL-193103", "label": "PREG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16581", "formula": "C21H32O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193124", "reactome_id": "R-ALL-193124", "label": "17aHPREG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28750", "formula": "C21H32O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid 17-alpha-monooxygenase activity of CYP17A1 [endoplasmic reticulum membrane]", "activity_id": "193113", "ec_numbers": [ "1.14.14.19" ], "accessions": [ "P05093" ] } ], "citations": [ { "id": "PMID:10406467", "title": "Molecular modeling of human P450c17 (17alpha-hydroxylase/17,20-lyase): insights into reaction mechanisms and effects of mutations", "journal": "Mol Endocrinol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10406467/" } ], "x": 4451.333333333333, "y": 1583.0984848484848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193070", "label": "CYP17A1 cleaves 17aHPREG to DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193070", "summary": "CYP17A1 cleaves 17aHPREG to DHA is a Reactome release 97 curated reaction event. The source record connects 17aHPREG, O2, NADPH to NADP+, H2O, CH3CHO in cytosol · endoplasmic reticulum membrane.", "equation": "17aHPREG + O2 + NADPH + H+ → NADP+ + H2O + CH3CHO + DHEA", "inputs": [ { "node_id": "R-ALL-193124", "reactome_id": "R-ALL-193124", "label": "17aHPREG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28750", "formula": "C21H32O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29510", "reactome_id": "R-ALL-29510", "label": "CH3CHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-176626", "reactome_id": "R-ALL-176626", "label": "DHEA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28689", "formula": "C19H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid 17-alpha-monooxygenase activity of CYP17A1 [endoplasmic reticulum membrane]", "activity_id": "193113", "ec_numbers": [ "1.14.14.19" ], "accessions": [ "P05093" ] } ], "citations": [ { "id": "PMID:10406467", "title": "Molecular modeling of human P450c17 (17alpha-hydroxylase/17,20-lyase): insights into reaction mechanisms and effects of mutations", "journal": "Mol Endocrinol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10406467/" }, { "id": "PMID:9536209", "title": "A critical review of the origin and control of adrenal androgens", "journal": "Baillieres Clin Obstet Gynaecol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9536209/" } ], "x": 4730, "y": 1636.1439393939395, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193072", "label": "CYP17A1 17-hydroxylates P4 to 17aHPROG", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193072", "summary": "CYP17A1 17-hydroxylates P4 to 17aHPROG is a Reactome release 97 curated reaction event. The source record connects P4, O2, NADPH to 17aHPROG, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "P4 + O2 + NADPH + H+ → 17aHPROG + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-193055", "reactome_id": "R-ALL-193055", "label": "P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17026", "formula": "C21H30O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193092", "reactome_id": "R-ALL-193092", "label": "17aHPROG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17252", "formula": "C21H30O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid 17-alpha-monooxygenase activity of CYP17A1 [endoplasmic reticulum membrane]", "activity_id": "193113", "ec_numbers": [ "1.14.14.19" ], "accessions": [ "P05093" ] } ], "citations": [ { "id": "PMID:10406467", "title": "Molecular modeling of human P450c17 (17alpha-hydroxylase/17,20-lyase): insights into reaction mechanisms and effects of mutations", "journal": "Mol Endocrinol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10406467/" } ], "x": 4730, "y": 1653.8257575757575, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193073", "label": "HSD2B1 dimer, HSD3B2 dimer isomerise DHA to ANDST", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193073", "summary": "HSD2B1 dimer, HSD3B2 dimer isomerise DHA to ANDST is a Reactome release 97 curated reaction event. The source record connects NAD+, DHEA to ANDST, NADH, H+ in cytosol · endoplasmic reticulum membrane.", "equation": "NAD+ + DHEA → ANDST + NADH + H+", "inputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-176626", "reactome_id": "R-ALL-176626", "label": "DHEA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28689", "formula": "C19H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193079", "reactome_id": "R-ALL-193079", "label": "ANDST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16422", "formula": "C19H26O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid Delta-isomerase activity of HSD3B1 dimer, HSD3B2 dimer [endoplasmic reticulum membrane]", "activity_id": "193169", "ec_numbers": [ "5.3.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2243100", "title": "Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2243100/" }, { "id": "PMID:9536209", "title": "A critical review of the origin and control of adrenal androgens", "journal": "Baillieres Clin Obstet Gynaecol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9536209/" } ], "x": 4451.333333333333, "y": 1695.0833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193097", "label": "Pregnenolone translocates from the mitochondrial matrix to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "mitochondrial matrix · cytosol", "compartment_ids": [ "GO:0005759", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193097", "summary": "Pregnenolone translocates from the mitochondrial matrix to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PREG to PREG in mitochondrial matrix · cytosol.", "equation": "PREG → PREG", "inputs": [ { "node_id": "R-ALL-193164", "reactome_id": "R-ALL-193164", "label": "PREG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16581", "formula": "C21H32O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193103", "reactome_id": "R-ALL-193103", "label": "PREG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16581", "formula": "C21H32O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:6264249", "title": "Cytosol stimulation of pregnenolone synthesis by isolated adrenal mitochondria", "journal": "Life Sci.", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/6264249/" } ], "x": 5008.666666666667, "y": 1836.5378787878788, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193099", "label": "CYP17A1 cleaves 17aHPROG to ANDST", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193099", "summary": "CYP17A1 cleaves 17aHPROG to ANDST is a Reactome release 97 curated reaction event. The source record connects 17aHPROG, O2, NADPH to ANDST, NADP+, H2O in cytosol · endoplasmic reticulum membrane.", "equation": "17aHPROG + O2 + NADPH + H+ → ANDST + NADP+ + H2O + CH3CHO", "inputs": [ { "node_id": "R-ALL-193092", "reactome_id": "R-ALL-193092", "label": "17aHPROG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17252", "formula": "C21H30O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193079", "reactome_id": "R-ALL-193079", "label": "ANDST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16422", "formula": "C19H26O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29510", "reactome_id": "R-ALL-29510", "label": "CH3CHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid 17-alpha-monooxygenase activity of CYP17A1 [endoplasmic reticulum membrane]", "activity_id": "193113", "ec_numbers": [ "1.14.14.19" ], "accessions": [ "P05093" ] } ], "citations": [ { "id": "PMID:10406467", "title": "Molecular modeling of human P450c17 (17alpha-hydroxylase/17,20-lyase): insights into reaction mechanisms and effects of mutations", "journal": "Mol Endocrinol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10406467/" } ], "x": 5008.666666666667, "y": 1636.1439393939395, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193101", "label": "CYP11A1 cleaves 20a,22b-DHCHOL", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:34344" ], "source_url": "https://reactome.org/content/detail/R-HSA-193101", "summary": "CYP11A1 cleaves 20a,22b-DHCHOL is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 20a,22b-DHCHOL, O2, CYP11A1:FDXR:FDX1,FDX1L (red.) to PREG, ISCAL, H2O in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:34344.", "equation": "20a,22b-DHCHOL + O2 + 2 CYP11A1:FDXR:FDX1,FDX1L (red.) + 2 NADPH + 2 H+ → PREG + ISCAL + 2 H2O + 2 CYP11A1:FDXR:FDX1,FDX1L (ox.) + 2 NADP+", "inputs": [ { "node_id": "R-ALL-193086", "reactome_id": "R-ALL-193086", "label": "20a,22b-DHCHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:1294", "formula": "C27H46O3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-5580249", "reactome_id": "R-HSA-5580249", "label": "CYP11A1:FDXR:FDX1,FDX1L (red.)", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193164", "reactome_id": "R-ALL-193164", "label": "PREG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16581", "formula": "C21H32O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193046", "reactome_id": "R-ALL-193046", "label": "ISCAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17998", "formula": "C6H12O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-5580259", "reactome_id": "R-HSA-5580259", "label": "CYP11A1:FDXR:FDX1,FDX1L (ox.)", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cholesterol monooxygenase (side-chain-cleaving) activity of CYP11A1:FDXR:FDX1,FDX1L (red.) [mitochondrial matrix]", "activity_id": "193154", "ec_numbers": [ "1.14.15.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:9578606", "title": "Expression of catalytically active human cytochrome p450scc in Escherichia coli and mutagenesis of isoleucine-462", "journal": "Arch Biochem Biophys", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9578606/" }, { "id": "PMID:3024157", "title": "Human cholesterol side-chain cleavage enzyme, P450scc: cDNA cloning, assignment of the gene to chromosome 15, and expression in the placenta", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3024157/" }, { "id": "PMID:21636783", "title": "Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase system", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21636783/" } ], "x": 4451.333333333333, "y": 1571.310606060606, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193143", "label": "CYP19A1 hydroxylates TEST to EST17b", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193143", "summary": "CYP19A1 hydroxylates TEST to EST17b is a Reactome release 97 curated reaction event. The source record connects TEST, NADPH, H+ to EST17b, NADP+, HCOOH in cytosol · endoplasmic reticulum membrane.", "equation": "TEST + NADPH + H+ + O2 → EST17b + NADP+ + HCOOH + H2O", "inputs": [ { "node_id": "R-ALL-193057", "reactome_id": "R-ALL-193057", "label": "TEST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17347", "formula": "C19H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193132", "reactome_id": "R-ALL-193132", "label": "EST17b", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16469", "formula": "C18H24O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid hydroxylase activity of CYP19A1 [endoplasmic reticulum membrane]", "activity_id": "193170", "ec_numbers": null, "accessions": [ "P11511" ] } ], "citations": [ { "id": "PMID:8076586", "title": "Aromatase cytochrome P450, the enzyme responsible for estrogen biosynthesis", "journal": "Endocr Rev", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8076586/" }, { "id": "PMID:2171939", "title": "Structural and functional characterization of human aromatase P-450 gene", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2171939/" } ], "x": 4451.333333333333, "y": 1642.0378787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193362", "label": "ABCB11 transports bile salts from cytosol to extracellular region", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:50056", "RHEA:50052", "RHEA:50064", "RHEA:50060" ], "source_url": "https://reactome.org/content/detail/R-HSA-193362", "summary": "ABCB11 transports bile salts from cytosol to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects bile salts, ATP, H2O to bile salts, ADP, Pi in plasma membrane and cross-references RHEA:50056, RHEA:50052, RHEA:50064, RHEA:50060.", "equation": "bile salts + ATP + H2O → bile salts + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-193416", "reactome_id": "R-ALL-193416", "label": "bile salts", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193479", "reactome_id": "R-ALL-193479", "label": "bile salts", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ABC-type bile acid transporter activity of ABCB11 [plasma membrane]", "activity_id": "193367", "ec_numbers": null, "accessions": [ "O95342" ] } ], "citations": [ { "id": "PMID:12404240", "title": "Functional expression of the canalicular bile salt export pump of human liver", "journal": "Gastroenterology", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12404240/" }, { "id": "PMID:12404239", "title": "The human bile salt export pump: characterization of substrate specificity and identification of inhibitors", "journal": "Gastroenterology", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12404239/" } ], "x": 4451.333333333333, "y": 1535.9469696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193369", "label": "25(S) DHCA-CoA is dehydrogenated to 25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193369", "summary": "25(S) DHCA-CoA is dehydrogenated to 25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 25(S) DHCA-CoA, O2 to 25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA, H2O2 in peroxisomal matrix.", "equation": "25(S) DHCA-CoA + O2 → 25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA + H2O2", "inputs": [ { "node_id": "R-ALL-193442", "reactome_id": "R-ALL-193442", "label": "25(S) DHCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15494", "formula": "C48H80N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193459", "reactome_id": "R-ALL-193459", "label": "25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27393", "formula": "C48H78N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA oxidase activity of ACOX2:FAD [peroxisomal matrix]", "activity_id": "192336", "ec_numbers": [ "1.3.3.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:8943006", "title": "Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8943006/" }, { "id": "PMID:8387517", "title": "The CoA esters of 2-methyl-branched chain fatty acids and of the bile acid intermediates di- and trihydroxycoprostanic acids are oxidized by one single peroxisomal branched chain acyl-CoA oxidase in human liver and kidney", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8387517/" } ], "x": 5008.666666666667, "y": 1712.7651515151515, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193385", "label": "Hydrolysis of choloyl-CoA to cholate and CoASH", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:14542" ], "source_url": "https://reactome.org/content/detail/R-HSA-193385", "summary": "Hydrolysis of choloyl-CoA to cholate and CoASH is a Reactome release 97 curated reaction event. The source record connects H2O, choloyl-CoA to CCA, CoA-SH, H+ in peroxisomal matrix and cross-references RHEA:14542.", "equation": "H2O + choloyl-CoA → CCA + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-192356", "reactome_id": "R-ALL-192356", "label": "choloyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57373", "formula": "C45H70N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193451", "reactome_id": "R-ALL-193451", "label": "CCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29747", "formula": "C24H39O5", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "choloyl-CoA hydrolase activity of ACOT8 [peroxisomal matrix]", "activity_id": "193529", "ec_numbers": [ "3.1.2.27" ], "accessions": [ "O14734" ] } ], "citations": [ { "id": "PMID:11673457", "title": "Characterization of an acyl-coA thioesterase that functions as a major regulator of peroxisomal lipid metabolism", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11673457/" }, { "id": "PMID:10092594", "title": "Identification of peroxisomal acyl-CoA thioesterases in yeast and humans", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10092594/" } ], "x": 5008.666666666667, "y": 1553.628787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193393", "label": "5beta-cholestan-3alpha, 7alpha-diol is hydroxylated to 5beta-cholestan-3alpha, 7alpha, 26-triol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193393", "summary": "5beta-cholestan-3alpha, 7alpha-diol is hydroxylated to 5beta-cholestan-3alpha, 7alpha, 26-triol is a Reactome release 97 curated reaction event. The source record connects 5beta-cholestan-3alpha, 7alpha-diol, NADPH, H+ to 5beta-cholestan-3alpha,7alpha,26-triol, H2O, NADP+ in mitochondrial matrix.", "equation": "5beta-cholestan-3alpha, 7alpha-diol + NADPH + H+ + O2 → 5beta-cholestan-3alpha,7alpha,26-triol + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-193436", "reactome_id": "R-ALL-193436", "label": "5beta-cholestan-3alpha, 7alpha-diol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28047", "formula": "C27H48O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193387", "reactome_id": "R-ALL-193387", "label": "5beta-cholestan-3alpha,7alpha,26-triol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28540", "formula": "C27H48O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:2019602", "title": "Mutations in the bile acid biosynthetic enzyme sterol 27-hydroxylase underlie cerebrotendinous xanthomatosis", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2019602/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 5008.666666666667, "y": 1653.8257575757575, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193399", "label": "Cholate is translocated from the peroxisomal matrix to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal membrane", "compartment_ids": [ "GO:0005778" ], "rhea_ids": [ "RHEA:72683" ], "source_url": "https://reactome.org/content/detail/R-HSA-193399", "summary": "Cholate is translocated from the peroxisomal matrix to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CCA to CCA in peroxisomal membrane and cross-references RHEA:72683.", "equation": "CCA → CCA", "inputs": [ { "node_id": "R-ALL-193451", "reactome_id": "R-ALL-193451", "label": "CCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29747", "formula": "C24H39O5", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193473", "reactome_id": "R-ALL-193473", "label": "CCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29747", "formula": "C24H39O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 4451.333333333333, "y": 1636.1439393939395, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193401", "label": "THCA is conjugated with Coenzyme A (SLC27A2 VLCS)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:22977" ], "source_url": "https://reactome.org/content/detail/R-HSA-193401", "summary": "THCA is conjugated with Coenzyme A (SLC27A2 VLCS) is a Reactome release 97 curated reaction event. The source record connects THCA, ATP, CoA-SH to 25(R) THCA-CoA, AMP, PPi in cytosol · endoplasmic reticulum membrane and cross-references RHEA:22977.", "equation": "THCA + ATP + CoA-SH → 25(R) THCA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-192013", "reactome_id": "R-ALL-192013", "label": "THCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18402", "formula": "C27H46O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192096", "reactome_id": "R-ALL-192096", "label": "25(R) THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58677", "formula": "C48H76N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of SLC27A2 [endoplasmic reticulum membrane]", "activity_id": "193506", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "O14975" ] } ], "citations": [ { "id": "PMID:10479480", "title": "Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization of a second enzymatically active protein", "journal": "Mol Genet Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10479480/" }, { "id": "PMID:10749848", "title": "The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10749848/" }, { "id": "PMID:11980911", "title": "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980911/" } ], "x": 5008.666666666667, "y": 1594.8863636363637, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193407", "label": "DHCA is conjugated with Coenzyme A (SLC27A5 BACS)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193407", "summary": "DHCA is conjugated with Coenzyme A (SLC27A5 BACS) is a Reactome release 97 curated reaction event. The source record connects ATP, CoA-SH, DHCA to 25(R) DHCA-CoA, AMP, PPi in cytosol · endoplasmic reticulum membrane.", "equation": "ATP + CoA-SH + DHCA → 25(R) DHCA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193505", "reactome_id": "R-ALL-193505", "label": "DHCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16577", "formula": "C27H46O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193457", "reactome_id": "R-ALL-193457", "label": "25(R) DHCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15494", "formula": "C48H80N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of SLC27A5 [endoplasmic reticulum membrane]", "activity_id": "192026", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "Q9Y2P5" ] } ], "citations": [ { "id": "PMID:10479480", "title": "Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization of a second enzymatically active protein", "journal": "Mol Genet Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10479480/" }, { "id": "PMID:10749848", "title": "The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10749848/" }, { "id": "PMID:11980911", "title": "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980911/" } ], "x": 4451.333333333333, "y": 1683.2954545454545, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193424", "label": "DHCA is conjugated with Coenzyme A (SLC27A2 VLCS)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193424", "summary": "DHCA is conjugated with Coenzyme A (SLC27A2 VLCS) is a Reactome release 97 curated reaction event. The source record connects DHCA, ATP, CoA-SH to 25(R) DHCA-CoA, AMP, PPi in cytosol · endoplasmic reticulum membrane.", "equation": "DHCA + ATP + CoA-SH → 25(R) DHCA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-193505", "reactome_id": "R-ALL-193505", "label": "DHCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16577", "formula": "C27H46O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193457", "reactome_id": "R-ALL-193457", "label": "25(R) DHCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15494", "formula": "C48H80N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of SLC27A2 [endoplasmic reticulum membrane]", "activity_id": "193506", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "O14975" ] } ], "citations": [ { "id": "PMID:10479480", "title": "Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization of a second enzymatically active protein", "journal": "Mol Genet Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10479480/" }, { "id": "PMID:10749848", "title": "The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10749848/" }, { "id": "PMID:11980911", "title": "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980911/" } ], "x": 4730, "y": 1683.2954545454545, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193452", "label": "Isomerization of 25(R) DHCA-CoA to 25(S) DHCA-CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193452", "summary": "Isomerization of 25(R) DHCA-CoA to 25(S) DHCA-CoA is a Reactome release 97 curated reaction event. The source record connects 25(R) DHCA-CoA to 25(S) DHCA-CoA in peroxisomal matrix.", "equation": "25(R) DHCA-CoA → 25(S) DHCA-CoA", "inputs": [ { "node_id": "R-ALL-193429", "reactome_id": "R-ALL-193429", "label": "25(R) DHCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15494", "formula": "C48H80N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193442", "reactome_id": "R-ALL-193442", "label": "25(S) DHCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15494", "formula": "C48H80N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "racemase and epimerase activity of AMACR [peroxisomal matrix]", "activity_id": "191973", "ec_numbers": [ "5.1" ], "accessions": [ "Q9UHK6" ] } ], "citations": [ { "id": "PMID:7649182", "title": "Purification and characterization of an alpha-methylacyl-CoA racemase from human liver", "journal": "Eur J Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7649182/" }, { "id": "PMID:11060344", "title": "Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA racemase in humans", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11060344/" }, { "id": "PMID:10655068", "title": "Mutations in the gene encoding peroxisomal alpha-methylacyl-CoA racemase cause adult-onset sensory motor neuropathy", "journal": "Nat Genet", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10655068/" } ], "x": 4730, "y": 1724.5530303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193455", "label": "(24R, 25R) 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-one-CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:47089", "RHEA:47088" ], "source_url": "https://reactome.org/content/detail/R-HSA-193455", "summary": "(24R, 25R) 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA is oxidized to 3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-one-CoA is a Reactome release 97 curated reaction event. The source record connects (24R, 25R) 3a,7a,12a,24-tetrahydroxy-5b-cholestanoyl-CoA, NAD+ to 3a,7a,12a-trihydroxy-5b-cholest-24-one-CoA, NADH, H+ in peroxisomal matrix and cross-references RHEA:47089, RHEA:47088.", "equation": "(24R, 25R) 3a,7a,12a,24-tetrahydroxy-5b-cholestanoyl-CoA + NAD+ → 3a,7a,12a-trihydroxy-5b-cholest-24-one-CoA + NADH + H+", "inputs": [ { "node_id": "R-ALL-193422", "reactome_id": "R-ALL-193422", "label": "(24R, 25R) 3a,7a,12a,24-tetrahydroxy-5b-cholestanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59807", "formula": "C48H76N7O21P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192307", "reactome_id": "R-ALL-192307", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-192339", "reactome_id": "R-ALL-192339", "label": "3a,7a,12a-trihydroxy-5b-cholest-24-one-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58507", "formula": "C48H74N7O21P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192305", "reactome_id": "R-ALL-192305", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "193365", "ec_numbers": [ "1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:8902629", "title": "Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase", "journal": "J Biochem (Tokyo)", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8902629/" }, { "id": "PMID:10706581", "title": "Peroxisomal fatty acid oxidation disorders and 58 kDa sterol carrier protein X (SCPx). Activity measurements in liver and fibroblasts using a newly developed method.", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10706581/" } ], "x": 4451.333333333333, "y": 1559.5227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193460", "label": "3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193460", "summary": "3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestanoate (DHCA) is a Reactome release 97 curated reaction event. The source record connects 3alpha,7alpha-dihydroxy-5beta-cholestan-26-al, NADPH, H+ to DHCA, NADP+, H2O in mitochondrial matrix.", "equation": "3alpha,7alpha-dihydroxy-5beta-cholestan-26-al + NADPH + H+ + O2 → DHCA + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-193435", "reactome_id": "R-ALL-193435", "label": "3alpha,7alpha-dihydroxy-5beta-cholestan-26-al", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27428", "formula": "C27H46O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193440", "reactome_id": "R-ALL-193440", "label": "DHCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16577", "formula": "C27H46O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 4451.333333333333, "y": 1659.719696969697, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193482", "label": "25(R) DHCA-CoA is translocated from the cytosol to the peroxisome", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal membrane", "compartment_ids": [ "GO:0005778" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193482", "summary": "25(R) DHCA-CoA is translocated from the cytosol to the peroxisome is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 25(R) DHCA-CoA, ATP, H2O to 25(R) DHCA-CoA, ADP, Pi in peroxisomal membrane.", "equation": "25(R) DHCA-CoA + ATP + H2O → 25(R) DHCA-CoA + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-193457", "reactome_id": "R-ALL-193457", "label": "25(R) DHCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15494", "formula": "C48H80N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193429", "reactome_id": "R-ALL-193429", "label": "25(R) DHCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15494", "formula": "C48H80N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ATPase-coupled transmembrane transporter activity of ABCD3 dimer [peroxisomal membrane]", "activity_id": "9987431", "ec_numbers": [ "7.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:25168382", "title": "A novel bile acid biosynthesis defect due to a deficiency of peroxisomal ABCD3", "journal": "Hum Mol Genet", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25168382/" }, { "id": "PMID:17609205", "title": "Live cell FRET microscopy: homo- and heterodimerization of two human peroxisomal ABC transporters, the adrenoleukodystrophy protein (ALDP, ABCD1) and PMP70 (ABCD3)", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17609205/" }, { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" }, { "id": "PMID:12966071", "title": "ATP-dependent transport of bile acid intermediates across rat liver peroxisomal membranes", "journal": "J Biochem (Tokyo)", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12966071/" } ], "x": 4730, "y": 1659.719696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193491", "label": "Chenodeoxycholoyl CoA reacts with glycine or taurine to form glycochenodeoxycholate or taurochenodeoxycholate", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:49789", "RHEA:49785" ], "source_url": "https://reactome.org/content/detail/R-HSA-193491", "summary": "Chenodeoxycholoyl CoA reacts with glycine or taurine to form glycochenodeoxycholate or taurochenodeoxycholate is a Reactome release 97 curated reaction event. The source record connects chenodeoxycholoyl-CoA, Gly, TAU to CoA-SH, chenodeoxycholate bile salts, H+ in peroxisomal matrix and cross-references RHEA:49789, RHEA:49785.", "equation": "chenodeoxycholoyl-CoA + Gly, TAU → CoA-SH + chenodeoxycholate bile salts + H+", "inputs": [ { "node_id": "R-ALL-193472", "reactome_id": "R-ALL-193472", "label": "chenodeoxycholoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62989", "formula": "C45H70N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193463", "reactome_id": "R-ALL-193463", "label": "Gly, TAU", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193453", "reactome_id": "R-ALL-193453", "label": "chenodeoxycholate bile salts", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyltransferase activity, transferring groups other than amino-acyl groups of BAAT [peroxisomal matrix]", "activity_id": "193371", "ec_numbers": [ "2.3.1" ], "accessions": [ "Q14032" ] } ], "citations": [ { "id": "PMID:8034703", "title": "Glycine and taurine conjugation of bile acids by a single enzyme. Molecular cloning and expression of human liver bile acid CoA:amino acid N-acyltransferase.", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8034703/" } ], "x": 4730, "y": 1541.840909090909, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193497", "label": "5beta-cholestan-3alpha, 7alpha, 26-triol is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193497", "summary": "5beta-cholestan-3alpha, 7alpha, 26-triol is oxidized to 3alpha, 7alpha-dihydroxy-5beta-cholestan-26-al is a Reactome release 97 curated reaction event. The source record connects 5beta-cholestan-3alpha,7alpha,26-triol, NADP+ to 3alpha,7alpha-dihydroxy-5beta-cholestan-26-al, H+, NADPH in mitochondrial matrix.", "equation": "5beta-cholestan-3alpha,7alpha,26-triol + NADP+ → 3alpha,7alpha-dihydroxy-5beta-cholestan-26-al + H+ + NADPH", "inputs": [ { "node_id": "R-ALL-193387", "reactome_id": "R-ALL-193387", "label": "5beta-cholestan-3alpha,7alpha,26-triol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28540", "formula": "C27H48O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193435", "reactome_id": "R-ALL-193435", "label": "3alpha,7alpha-dihydroxy-5beta-cholestan-26-al", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27428", "formula": "C27H46O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 4730, "y": 1695.0833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193508", "label": "(24R, 25R) 3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA is oxidized to 3alpha,7alpha-dihydroxy-5beta-cholest-24-one-CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:47405" ], "source_url": "https://reactome.org/content/detail/R-HSA-193508", "summary": "(24R, 25R) 3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA is oxidized to 3alpha,7alpha-dihydroxy-5beta-cholest-24-one-CoA is a Reactome release 97 curated reaction event. The source record connects 3,7,24THCA-CoA, NAD+ to 3a,7a-dihydroxy-5b-cholest-24-one-CoA, NADH, H+ in peroxisomal matrix and cross-references RHEA:47405.", "equation": "3,7,24THCA-CoA + NAD+ → 3a,7a-dihydroxy-5b-cholest-24-one-CoA + NADH + H+", "inputs": [ { "node_id": "R-ALL-193732", "reactome_id": "R-ALL-193732", "label": "3,7,24THCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27403", "formula": "C48H80N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192307", "reactome_id": "R-ALL-192307", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193403", "reactome_id": "R-ALL-193403", "label": "3a,7a-dihydroxy-5b-cholest-24-one-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87704", "formula": "C48H74N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192305", "reactome_id": "R-ALL-192305", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "193365", "ec_numbers": [ "1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:8902629", "title": "Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase", "journal": "J Biochem (Tokyo)", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8902629/" } ], "x": 5008.666666666667, "y": 1600.780303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193519", "label": "DHCA is translocated from the mitochondrial matrix to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193519", "summary": "DHCA is translocated from the mitochondrial matrix to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DHCA to DHCA in mitochondrial inner membrane.", "equation": "DHCA → DHCA", "inputs": [ { "node_id": "R-ALL-193440", "reactome_id": "R-ALL-193440", "label": "DHCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16577", "formula": "C27H46O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193505", "reactome_id": "R-ALL-193505", "label": "DHCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16577", "formula": "C27H46O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 4451.333333333333, "y": 1842.4318181818182, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193533", "label": "Thiolysis of 3alpha,7alpha-dihydroxy-5beta-cholan-24-one-CoA yields chenodeoxycholoyl-CoA (3alpha,7alpha-dihydroxy-5beta-cholan-24-one-CoA) and propionyl CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193533", "summary": "Thiolysis of 3alpha,7alpha-dihydroxy-5beta-cholan-24-one-CoA yields chenodeoxycholoyl-CoA (3alpha,7alpha-dihydroxy-5beta-cholan-24-one-CoA) and propionyl CoA is a Reactome release 97 curated reaction event. The source record connects 3a,7a-dihydroxy-5b-cholest-24-one-CoA, CoA-SH to chenodeoxycholoyl-CoA, propionyl CoA in peroxisomal matrix.", "equation": "3a,7a-dihydroxy-5b-cholest-24-one-CoA + CoA-SH → chenodeoxycholoyl-CoA + propionyl CoA", "inputs": [ { "node_id": "R-ALL-193403", "reactome_id": "R-ALL-193403", "label": "3a,7a-dihydroxy-5b-cholest-24-one-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:87704", "formula": "C48H74N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193472", "reactome_id": "R-ALL-193472", "label": "chenodeoxycholoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62989", "formula": "C45H70N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192314", "reactome_id": "R-ALL-192314", "label": "propionyl CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57392", "formula": "C24H36N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "propanoyl-CoA C-acyltransferase activity of SCP2-1 [peroxisomal matrix]", "activity_id": "192310", "ec_numbers": [ "2.3.1.176" ], "accessions": [ "P22307" ] } ], "citations": [ { "id": "PMID:1703300", "title": "Cloning and expression of a cDNA encoding human sterol carrier protein 2", "journal": "Proc Natl Acad Sci U S A", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1703300/" }, { "id": "PMID:10706581", "title": "Peroxisomal fatty acid oxidation disorders and 58 kDa sterol carrier protein X (SCPx). Activity measurements in liver and fibroblasts using a newly developed method.", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10706581/" } ], "x": 4451.333333333333, "y": 1718.659090909091, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193535", "label": "25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA is hydrated to (24R, 25R) 3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193535", "summary": "25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA is hydrated to (24R, 25R) 3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA, H2O to 3,7,24THCA-CoA in peroxisomal matrix.", "equation": "25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA + H2O → 3,7,24THCA-CoA", "inputs": [ { "node_id": "R-ALL-193459", "reactome_id": "R-ALL-193459", "label": "25(S) 3alpha,7alpha-dihydroxy-5beta-cholest-24-enoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27393", "formula": "C48H78N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193732", "reactome_id": "R-ALL-193732", "label": "3,7,24THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27403", "formula": "C48H80N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase activity of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "192350", "ec_numbers": [ "4.2.1.107" ], "accessions": [] } ], "citations": [ { "id": "PMID:8902629", "title": "Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase", "journal": "J Biochem (Tokyo)", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8902629/" } ], "x": 5008.666666666667, "y": 1718.659090909091, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193537", "label": "5beta-cholestan-3alpha, 7alpha-diol is translocated from the cytosol to the mitochondrial matrix", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193537", "summary": "5beta-cholestan-3alpha, 7alpha-diol is translocated from the cytosol to the mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 5beta-cholestan-3alpha,7alpha-diol to 5beta-cholestan-3alpha, 7alpha-diol in mitochondrial inner membrane.", "equation": "5beta-cholestan-3alpha,7alpha-diol → 5beta-cholestan-3alpha, 7alpha-diol", "inputs": [ { "node_id": "R-ALL-192030", "reactome_id": "R-ALL-192030", "label": "5beta-cholestan-3alpha,7alpha-diol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28047", "formula": "C27H48O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193436", "reactome_id": "R-ALL-193436", "label": "5beta-cholestan-3alpha, 7alpha-diol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28047", "formula": "C27H48O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol transmembrane transporter activity of unknown START protein [mitochondrial inner membrane]", "activity_id": "9948409", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 5008.666666666667, "y": 1724.5530303030303, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193711", "label": "3,7,24THCA is conjugated with Coenzyme A (SLC27A5 BACS)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193711", "summary": "3,7,24THCA is conjugated with Coenzyme A (SLC27A5 BACS) is a Reactome release 97 curated reaction event. The source record connects 3,7,24THCA, ATP, CoA-SH to 3,7,24THCA-CoA, AMP, PPi in cytosol · endoplasmic reticulum membrane.", "equation": "3,7,24THCA + ATP + CoA-SH → 3,7,24THCA-CoA + AMP + PPi + H2O", "inputs": [ { "node_id": "R-ALL-193778", "reactome_id": "R-ALL-193778", "label": "3,7,24THCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48736", "formula": "C27H46O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193739", "reactome_id": "R-ALL-193739", "label": "3,7,24THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27403", "formula": "C48H80N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of SLC27A5 [endoplasmic reticulum membrane]", "activity_id": "192026", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "Q9Y2P5" ] } ], "citations": [ { "id": "PMID:10479480", "title": "Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization of a second enzymatically active protein", "journal": "Mol Genet Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10479480/" }, { "id": "PMID:10749848", "title": "The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10749848/" }, { "id": "PMID:11980911", "title": "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980911/" } ], "x": 5008.666666666667, "y": 1659.719696969697, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193715", "label": "5beta-cholestan-3alpha,7alpha,24(S)-triol is translocated from the cytosol to the mitochondrial matrix", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193715", "summary": "5beta-cholestan-3alpha,7alpha,24(S)-triol is translocated from the cytosol to the mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 5beta-cholestan-3alpha,7alpha,24(S)-triol to 5β-CHOL3α,7α,24(s)-triol in mitochondrial inner membrane.", "equation": "5beta-cholestan-3alpha,7alpha,24(S)-triol → 5β-CHOL3α,7α,24(s)-triol", "inputs": [ { "node_id": "R-ALL-213285", "reactome_id": "R-ALL-213285", "label": "5beta-cholestan-3alpha,7alpha,24(S)-triol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63831", "formula": "C27H48O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193720", "reactome_id": "R-ALL-193720", "label": "5β-CHOL3α,7α,24(s)-triol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63831", "formula": "C27H48O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol transmembrane transporter activity of unknown START protein [mitochondrial inner membrane]", "activity_id": "9948409", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 4451.333333333333, "y": 1730.4469696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193719", "label": "5beta-cholestan-3alpha,7alpha,24(S),27-tetrol is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193719", "summary": "5beta-cholestan-3alpha,7alpha,24(S),27-tetrol is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al is a Reactome release 97 curated reaction event. The source record connects 5bCHOL3a,7a,24(s),27-tetrol, O2, NADPH to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al, H2O, NADP+ in mitochondrial matrix.", "equation": "5bCHOL3a,7a,24(s),27-tetrol + O2 + NADPH + H+ → 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-193708", "reactome_id": "R-ALL-193708", "label": "5bCHOL3a,7a,24(s),27-tetrol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63833", "formula": "C27H48O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193716", "reactome_id": "R-ALL-193716", "label": "3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63848", "formula": "C27H46O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 4451.333333333333, "y": 1665.6136363636363, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193736", "label": "Isomerization of 3,7,24THCA-CoA to (24R, 25R) 3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193736", "summary": "Isomerization of 3,7,24THCA-CoA to (24R, 25R) 3alpha,7alpha,24-trihydroxy-5beta-cholestanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3,7,24THCA-CoA to 3,7,24THCA-CoA in peroxisomal matrix.", "equation": "3,7,24THCA-CoA → 3,7,24THCA-CoA", "inputs": [ { "node_id": "R-ALL-193732", "reactome_id": "R-ALL-193732", "label": "3,7,24THCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27403", "formula": "C48H80N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193732", "reactome_id": "R-ALL-193732", "label": "3,7,24THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27403", "formula": "C48H80N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "racemase and epimerase activity of AMACR [peroxisomal matrix]", "activity_id": "191973", "ec_numbers": [ "5.1" ], "accessions": [ "Q9UHK6" ] } ], "citations": [ { "id": "PMID:7649182", "title": "Purification and characterization of an alpha-methylacyl-CoA racemase from human liver", "journal": "Eur J Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7649182/" }, { "id": "PMID:11060344", "title": "Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA racemase in humans", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11060344/" }, { "id": "PMID:10655068", "title": "Mutations in the gene encoding peroxisomal alpha-methylacyl-CoA racemase cause adult-onset sensory motor neuropathy", "journal": "Nat Genet", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10655068/" } ], "x": 5008.666666666667, "y": 1742.2348484848485, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193737", "label": "3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestanoate (3,7,24THCA)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193737", "summary": "3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al is oxidized to 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestanoate (3,7,24THCA) is a Reactome release 97 curated reaction event. The source record connects 3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al, NADPH, H+ to 3,7,24THCA, NADP+, H2O in mitochondrial matrix.", "equation": "3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al + NADPH + H+ + O2 → 3,7,24THCA + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-193716", "reactome_id": "R-ALL-193716", "label": "3alpha,7alpha,24(S)-trihydroxy-5beta-cholestan-27-al", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63848", "formula": "C27H46O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193757", "reactome_id": "R-ALL-193757", "label": "3,7,24THCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:48736", "formula": "C27H46O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 4730, "y": 1665.6136363636363, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193743", "label": "3,7,24THCA is conjugated with Coenzyme A (SLC27A2 VLCS)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193743", "summary": "3,7,24THCA is conjugated with Coenzyme A (SLC27A2 VLCS) is a Reactome release 97 curated reaction event. The source record connects 3,7,24THCA, ATP, CoA-SH to 3,7,24THCA-CoA, AMP, PPi in cytosol · endoplasmic reticulum membrane.", "equation": "3,7,24THCA + ATP + CoA-SH → 3,7,24THCA-CoA + AMP + PPi + H2O", "inputs": [ { "node_id": "R-ALL-193778", "reactome_id": "R-ALL-193778", "label": "3,7,24THCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48736", "formula": "C27H46O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193739", "reactome_id": "R-ALL-193739", "label": "3,7,24THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27403", "formula": "C48H80N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of SLC27A2 [endoplasmic reticulum membrane]", "activity_id": "193506", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "O14975" ] } ], "citations": [ { "id": "PMID:10479480", "title": "Human liver-specific very-long-chain acyl-coenzyme A synthetase: cDNA cloning and characterization of a second enzymatically active protein", "journal": "Mol Genet Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10479480/" }, { "id": "PMID:10749848", "title": "The human liver-specific homolog of very long-chain acyl-CoA synthetase is cholate:CoA ligase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10749848/" }, { "id": "PMID:11980911", "title": "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11980911/" } ], "x": 5008.666666666667, "y": 1665.6136363636363, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193746", "label": "4-cholesten-7alpha,24(S)-diol-3-one is reduced to 5beta-cholestan-7alpha,24(S)-diol-3-one", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193746", "summary": "4-cholesten-7alpha,24(S)-diol-3-one is reduced to 5beta-cholestan-7alpha,24(S)-diol-3-one is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 4CHOL7a,24(S)DONE, NADPH, H+ to 5beta-cholestan-7alpha,24(S)-diol-3-one, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "4CHOL7a,24(S)DONE + NADPH + H+ → 5beta-cholestan-7alpha,24(S)-diol-3-one + NADP+", "inputs": [ { "node_id": "R-ALL-193724", "reactome_id": "R-ALL-193724", "label": "4CHOL7a,24(S)DONE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63838", "formula": "C27H44O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193760", "reactome_id": "R-ALL-193760", "label": "5beta-cholestan-7alpha,24(S)-diol-3-one", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63829", "formula": "C27H46O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of AKR1D1 [cytosol]", "activity_id": "192006", "ec_numbers": [ "1.1.1.2" ], "accessions": [ "P51857" ] } ], "citations": [ { "id": "PMID:7508385", "title": "Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7508385/" } ], "x": 5008.666666666667, "y": 1695.0833333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193753", "label": "3,7,24THCA-CoA is translocated from the cytosol to the peroxisome", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · peroxisomal matrix · peroxisomal membrane", "compartment_ids": [ "GO:0005829", "GO:0005782", "GO:0005778" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193753", "summary": "3,7,24THCA-CoA is translocated from the cytosol to the peroxisome is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 3,7,24THCA-CoA, ATP, H2O to 3,7,24THCA-CoA, ADP, H+ in cytosol · peroxisomal matrix · peroxisomal membrane.", "equation": "3,7,24THCA-CoA + ATP + H2O → 3,7,24THCA-CoA + ADP + H+ + Pi", "inputs": [ { "node_id": "R-ALL-193739", "reactome_id": "R-ALL-193739", "label": "3,7,24THCA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27403", "formula": "C48H80N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193732", "reactome_id": "R-ALL-193732", "label": "3,7,24THCA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27403", "formula": "C48H80N7O20P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ATPase-coupled transmembrane transporter activity of ABCD3 dimer [peroxisomal membrane]", "activity_id": "9987431", "ec_numbers": [ "7.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:25168382", "title": "A novel bile acid biosynthesis defect due to a deficiency of peroxisomal ABCD3", "journal": "Hum Mol Genet", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25168382/" }, { "id": "PMID:17609205", "title": "Live cell FRET microscopy: homo- and heterodimerization of two human peroxisomal ABC transporters, the adrenoleukodystrophy protein (ALDP, ABCD1) and PMP70 (ABCD3)", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17609205/" }, { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" }, { "id": "PMID:12966071", "title": "ATP-dependent transport of bile acid intermediates across rat liver peroxisomal membranes", "journal": "J Biochem (Tokyo)", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12966071/" } ], "x": 4451.333333333333, "y": 1671.5075757575758, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193758", "label": "5beta-cholestan-7alpha,24(S)-diol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,24(S)-triol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193758", "summary": "5beta-cholestan-7alpha,24(S)-diol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,24(S)-triol is a Reactome release 97 curated reaction event. The source record connects 5beta-cholestan-7alpha,24(S)-diol-3-one, NADPH, H+ to 5beta-cholestan-3alpha,7alpha,24(S)-triol, NADP+ in cytosol.", "equation": "5beta-cholestan-7alpha,24(S)-diol-3-one + NADPH + H+ → 5beta-cholestan-3alpha,7alpha,24(S)-triol + NADP+", "inputs": [ { "node_id": "R-ALL-193760", "reactome_id": "R-ALL-193760", "label": "5beta-cholestan-7alpha,24(S)-diol-3-one", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63829", "formula": "C27H46O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-213285", "reactome_id": "R-ALL-213285", "label": "5beta-cholestan-3alpha,7alpha,24(S)-triol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63831", "formula": "C27H48O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of Aldo-keto reductase family [cytosol]", "activity_id": "192098", "ec_numbers": [ "1.1.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11158055", "title": "Human types 1 and 3 3 alpha-hydroxysteroid dehydrogenases: differential lability and tissue distribution", "journal": "J Clin Endocrinol Metab", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11158055/" } ], "x": 4451.333333333333, "y": 1700.9772727272727, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193767", "label": "Influx of 24-hydroxycholesterol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193767", "summary": "Influx of 24-hydroxycholesterol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 24OH-CHOL to 24OH-CHOL in plasma membrane.", "equation": "24OH-CHOL → 24OH-CHOL", "inputs": [ { "node_id": "R-ALL-193717", "reactome_id": "R-ALL-193717", "label": "24OH-CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:34310", "formula": "C27H46O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192060", "reactome_id": "R-ALL-192060", "label": "24OH-CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:34310", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8790411", "title": "Cholesterol homeostasis in human brain: evidence for an age-dependent flux of 24S-hydroxycholesterol from the brain into the circulation", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8790411/" }, { "id": "PMID:9717719", "title": "Cholesterol homeostasis in human brain: turnover of 24S-hydroxycholesterol and evidence for a cerebral origin of most of this oxysterol in the circulation", "journal": "J Lipid Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9717719/" } ], "x": 4730, "y": 1842.4318181818182, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193776", "label": "Efflux of 24-hydroxycholesterol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193776", "summary": "Efflux of 24-hydroxycholesterol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 24OH-CHOL to 24OH-CHOL in plasma membrane.", "equation": "24OH-CHOL → 24OH-CHOL", "inputs": [ { "node_id": "R-ALL-192060", "reactome_id": "R-ALL-192060", "label": "24OH-CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:34310", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193717", "reactome_id": "R-ALL-193717", "label": "24OH-CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:34310", "formula": "C27H46O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8790411", "title": "Cholesterol homeostasis in human brain: evidence for an age-dependent flux of 24S-hydroxycholesterol from the brain into the circulation", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8790411/" }, { "id": "PMID:9717719", "title": "Cholesterol homeostasis in human brain: turnover of 24S-hydroxycholesterol and evidence for a cerebral origin of most of this oxysterol in the circulation", "journal": "J Lipid Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9717719/" } ], "x": 5008.666666666667, "y": 1842.4318181818182, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193786", "label": "3,7,24THCA is translocated from the mitochondrial matrix to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193786", "summary": "3,7,24THCA is translocated from the mitochondrial matrix to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 3,7,24THCA to 3,7,24THCA in mitochondrial inner membrane.", "equation": "3,7,24THCA → 3,7,24THCA", "inputs": [ { "node_id": "R-ALL-193757", "reactome_id": "R-ALL-193757", "label": "3,7,24THCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48736", "formula": "C27H46O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193778", "reactome_id": "R-ALL-193778", "label": "3,7,24THCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:48736", "formula": "C27H46O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 4451.333333333333, "y": 1848.3257575757575, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193789", "label": "Cholest-5-ene-3beta,7alpha,24(S)-triol is oxidized and isomerized to 4-cholesten-7alpha,24(S)-diol-3-one", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:47200", "RHEA:47201" ], "source_url": "https://reactome.org/content/detail/R-HSA-193789", "summary": "Cholest-5-ene-3beta,7alpha,24(S)-triol is oxidized and isomerized to 4-cholesten-7alpha,24(S)-diol-3-one is a Reactome release 97 curated reaction event. The source record connects CHOL7a,24(S)DIOL, NAD+ to 4CHOL7a,24(S)DONE, NADH, H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:47200, RHEA:47201.", "equation": "CHOL7a,24(S)DIOL + NAD+ → 4CHOL7a,24(S)DONE + NADH + H+", "inputs": [ { "node_id": "R-ALL-192189", "reactome_id": "R-ALL-192189", "label": "CHOL7a,24(S)DIOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37640", "formula": "C27H46O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193724", "reactome_id": "R-ALL-193724", "label": "4CHOL7a,24(S)DONE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63838", "formula": "C27H44O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of HSD3B7 [endoplasmic reticulum membrane]", "activity_id": "192106", "ec_numbers": [ "1.1.1" ], "accessions": [ "Q9H2F3" ] } ], "citations": [ { "id": "PMID:12679481", "title": "Molecular genetics of 3beta-hydroxy-Delta5-C27-steroid oxidoreductase deficiency in 16 patients with loss of bile acid synthesis and liver disease", "journal": "J Clin Endocrinol Metab", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12679481/" }, { "id": "PMID:11067870", "title": "The bile acid synthetic gene 3beta-hydroxy-Delta(5)-C(27)-steroid oxidoreductase is mutated in progressive intrahepatic cholestasis", "journal": "J Clin Invest", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11067870/" } ], "x": 4730, "y": 1559.5227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193792", "label": "CYP27A1 27-hydroxylates 5β-CHOL3α,7α,24(s)-triol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193792", "summary": "CYP27A1 27-hydroxylates 5β-CHOL3α,7α,24(s)-triol is a Reactome release 97 curated reaction event. The source record connects 5β-CHOL3α,7α,24(s)-triol, NADPH, H+ to 5bCHOL3a,7a,24(s),27-tetrol, H2O, NADP+ in mitochondrial matrix.", "equation": "5β-CHOL3α,7α,24(s)-triol + NADPH + H+ + O2 → 5bCHOL3a,7a,24(s),27-tetrol + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-193720", "reactome_id": "R-ALL-193720", "label": "5β-CHOL3α,7α,24(s)-triol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63831", "formula": "C27H48O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193708", "reactome_id": "R-ALL-193708", "label": "5bCHOL3a,7a,24(s),27-tetrol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63833", "formula": "C27H48O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H as acceptor of CYP27A1 [mitochondrial matrix]", "activity_id": "192037", "ec_numbers": [ "1.6.1" ], "accessions": [ "Q02318" ] } ], "citations": [ { "id": "PMID:1708392", "title": "Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1708392/" }, { "id": "PMID:2019602", "title": "Mutations in the bile acid biosynthetic enzyme sterol 27-hydroxylase underlie cerebrotendinous xanthomatosis", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2019602/" }, { "id": "PMID:9660774", "title": "Activities of recombinant human cytochrome P450c27 (CYP27) which produce intermediates of alternative bile acid biosynthetic pathways", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9660774/" } ], "x": 4730, "y": 1671.5075757575758, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193800", "label": "5Beta-cholestan-7alpha,12alpha,27-triol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193800", "summary": "5Beta-cholestan-7alpha,12alpha,27-triol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol is a Reactome release 97 curated reaction event. The source record connects 5beta-cholestan-7alpha,12alpha,27-triol-3-one, NADPH, H+ to 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol, NADP+ in cytosol.", "equation": "5beta-cholestan-7alpha,12alpha,27-triol-3-one + NADPH + H+ → 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol + NADP+", "inputs": [ { "node_id": "R-ALL-193829", "reactome_id": "R-ALL-193829", "label": "5beta-cholestan-7alpha,12alpha,27-triol-3-one", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48834", "formula": "C27H46O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193843", "reactome_id": "R-ALL-193843", "label": "5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17278", "formula": "C27H48O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of Aldo-keto reductase family [cytosol]", "activity_id": "192098", "ec_numbers": [ "1.1.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11158055", "title": "Human types 1 and 3 3 alpha-hydroxysteroid dehydrogenases: differential lability and tissue distribution", "journal": "J Clin Endocrinol Metab", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11158055/" } ], "x": 4730, "y": 1700.9772727272727, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193801", "label": "Influx of 27-hydroxycholesterol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193801", "summary": "Influx of 27-hydroxycholesterol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 27OH-CHOL to 27HCHOL in plasma membrane.", "equation": "27OH-CHOL → 27HCHOL", "inputs": [ { "node_id": "R-ALL-193804", "reactome_id": "R-ALL-193804", "label": "27OH-CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17703", "formula": "C27H46O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-176617", "reactome_id": "R-ALL-176617", "label": "27HCHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17703", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8790411", "title": "Cholesterol homeostasis in human brain: evidence for an age-dependent flux of 24S-hydroxycholesterol from the brain into the circulation", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8790411/" }, { "id": "PMID:9717719", "title": "Cholesterol homeostasis in human brain: turnover of 24S-hydroxycholesterol and evidence for a cerebral origin of most of this oxysterol in the circulation", "journal": "J Lipid Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9717719/" } ], "x": 4730, "y": 1848.3257575757575, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193808", "label": "5beta-cholestan-3alpha,7alpha,27-triol is translocated from the cytosol to the mitochondrial matrix", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193808", "summary": "5beta-cholestan-3alpha,7alpha,27-triol is translocated from the cytosol to the mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 5beta-cholestan-3alpha,7alpha,26-triol to 5beta-cholestan-3alpha,7alpha,26-triol in mitochondrial inner membrane.", "equation": "5beta-cholestan-3alpha,7alpha,26-triol → 5beta-cholestan-3alpha,7alpha,26-triol", "inputs": [ { "node_id": "R-ALL-193842", "reactome_id": "R-ALL-193842", "label": "5beta-cholestan-3alpha,7alpha,26-triol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28540", "formula": "C27H48O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193387", "reactome_id": "R-ALL-193387", "label": "5beta-cholestan-3alpha,7alpha,26-triol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28540", "formula": "C27H48O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol transmembrane transporter activity of unknown START protein [mitochondrial inner membrane]", "activity_id": "9948409", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 4730, "y": 1730.4469696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193812", "label": "Efflux of 27-hydroxycholesterol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193812", "summary": "Efflux of 27-hydroxycholesterol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 27OH-CHOL to 27OH-CHOL in plasma membrane.", "equation": "27OH-CHOL → 27OH-CHOL", "inputs": [ { "node_id": "R-ALL-193846", "reactome_id": "R-ALL-193846", "label": "27OH-CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17703", "formula": "C27H46O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193804", "reactome_id": "R-ALL-193804", "label": "27OH-CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17703", "formula": "C27H46O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [], "x": 4730, "y": 1866.0075757575758, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193816", "label": "Cholest-5-ene-3beta,7alpha,27-triol is oxidized and isomerized to 4-cholesten-7alpha,27-diol-3-one", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193816", "summary": "Cholest-5-ene-3beta,7alpha,27-triol is oxidized and isomerized to 4-cholesten-7alpha,27-diol-3-one is a Reactome release 97 curated reaction event. The source record connects Cholest-5-ene-3beta,7alpha,27-triol, NAD+ to 4CHOL7a,27DONE, NADH, H+ in cytosol · endoplasmic reticulum membrane.", "equation": "Cholest-5-ene-3beta,7alpha,27-triol + NAD+ → 4CHOL7a,27DONE + NADH + H+", "inputs": [ { "node_id": "R-ALL-192194", "reactome_id": "R-ALL-192194", "label": "Cholest-5-ene-3beta,7alpha,27-triol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18431", "formula": "C27H46O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193835", "reactome_id": "R-ALL-193835", "label": "4CHOL7a,27DONE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:48825", "formula": "C27H44O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of HSD3B7 [endoplasmic reticulum membrane]", "activity_id": "192106", "ec_numbers": [ "1.1.1" ], "accessions": [ "Q9H2F3" ] } ], "citations": [ { "id": "PMID:12679481", "title": "Molecular genetics of 3beta-hydroxy-Delta5-C27-steroid oxidoreductase deficiency in 16 patients with loss of bile acid synthesis and liver disease", "journal": "J Clin Endocrinol Metab", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12679481/" }, { "id": "PMID:11067870", "title": "The bile acid synthetic gene 3beta-hydroxy-Delta(5)-C(27)-steroid oxidoreductase is mutated in progressive intrahepatic cholestasis", "journal": "J Clin Invest", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11067870/" } ], "x": 5008.666666666667, "y": 1700.9772727272727, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193821", "label": "4-cholesten-7alpha,12alpha,27-triol-3-one is reduced to 5beta-cholestan-7alpha,12alpha,27-triol-3-one", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193821", "summary": "4-cholesten-7alpha,12alpha,27-triol-3-one is reduced to 5beta-cholestan-7alpha,12alpha,27-triol-3-one is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 4CHOL7a,12a,27TONE, NADPH, H+ to 5beta-cholestan-7alpha,12alpha,27-triol-3-one, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "4CHOL7a,12a,27TONE + NADPH + H+ → 5beta-cholestan-7alpha,12alpha,27-triol-3-one + NADP+", "inputs": [ { "node_id": "R-ALL-9617026", "reactome_id": "R-ALL-9617026", "label": "4CHOL7a,12a,27TONE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48833", "formula": "C27H44O4", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193829", "reactome_id": "R-ALL-193829", "label": "5beta-cholestan-7alpha,12alpha,27-triol-3-one", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:48834", "formula": "C27H46O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of AKR1D1 [cytosol]", "activity_id": "192006", "ec_numbers": [ "1.1.1.2" ], "accessions": [ "P51857" ] } ], "citations": [ { "id": "PMID:7508385", "title": "Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7508385/" } ], "x": 4451.333333333333, "y": 1706.871212121212, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193824", "label": "4-cholesten-7alpha,27-diol-3-one is reduced to 5beta-cholestan-7alpha,27-diol-3-one", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193824", "summary": "4-cholesten-7alpha,27-diol-3-one is reduced to 5beta-cholestan-7alpha,27-diol-3-one is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 4CHOL7a,27DONE, NADPH, H+ to 5beta-cholestan-7alpha,27-diol-3-one, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "4CHOL7a,27DONE + NADPH + H+ → 5beta-cholestan-7alpha,27-diol-3-one + NADP+", "inputs": [ { "node_id": "R-ALL-193835", "reactome_id": "R-ALL-193835", "label": "4CHOL7a,27DONE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48825", "formula": "C27H44O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193830", "reactome_id": "R-ALL-193830", "label": "5beta-cholestan-7alpha,27-diol-3-one", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:48778", "formula": "C27H46O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of AKR1D1 [cytosol]", "activity_id": "192006", "ec_numbers": [ "1.1.1.2" ], "accessions": [ "P51857" ] } ], "citations": [ { "id": "PMID:7508385", "title": "Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7508385/" } ], "x": 4730, "y": 1706.871212121212, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193832", "label": "5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol is translocated from the cytosol to the mitochondrial matrix", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193832", "summary": "5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol is translocated from the cytosol to the mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol to 5bCHOL3a,7a,12a,27-tetrol in mitochondrial inner membrane.", "equation": "5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol → 5bCHOL3a,7a,12a,27-tetrol", "inputs": [ { "node_id": "R-ALL-193843", "reactome_id": "R-ALL-193843", "label": "5beta-cholestan-3alpha,7alpha,12alpha,27-tetrol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17278", "formula": "C27H48O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192201", "reactome_id": "R-ALL-192201", "label": "5bCHOL3a,7a,12a,27-tetrol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17278", "formula": "C27H48O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol transmembrane transporter activity of unknown START protein [mitochondrial inner membrane]", "activity_id": "9948409", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12543708", "title": "The enzymes, regulation, and genetics of bile acid synthesis", "journal": "Annu Rev Biochem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12543708/" } ], "x": 5008.666666666667, "y": 1730.4469696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-193841", "label": "5beta-cholestan-7alpha,27-diol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,27-triol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193841", "summary": "5beta-cholestan-7alpha,27-diol-3-one is reduced to 5beta-cholestan-3alpha,7alpha,27-triol is a Reactome release 97 curated reaction event. The source record connects 5beta-cholestan-7alpha,27-diol-3-one, NADPH, H+ to 5beta-cholestan-3alpha,7alpha,26-triol, NADP+ in cytosol.", "equation": "5beta-cholestan-7alpha,27-diol-3-one + NADPH + H+ → 5beta-cholestan-3alpha,7alpha,26-triol + NADP+", "inputs": [ { "node_id": "R-ALL-193830", "reactome_id": "R-ALL-193830", "label": "5beta-cholestan-7alpha,27-diol-3-one", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48778", "formula": "C27H46O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193842", "reactome_id": "R-ALL-193842", "label": "5beta-cholestan-3alpha,7alpha,26-triol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28540", "formula": "C27H48O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of Aldo-keto reductase family [cytosol]", "activity_id": "192098", "ec_numbers": [ "1.1.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11158055", "title": "Human types 1 and 3 3 alpha-hydroxysteroid dehydrogenases: differential lability and tissue distribution", "journal": "J Clin Endocrinol Metab", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11158055/" } ], "x": 5008.666666666667, "y": 1706.871212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193845", "label": "CYP8B1 12-hydroxylates 4CHOL7a,27DONE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193845", "summary": "CYP8B1 12-hydroxylates 4CHOL7a,27DONE is a Reactome release 97 curated reaction event. The source record connects 4CHOL7a,27DONE, NADPH, H+ to 4CHOL7a,12a,27TONE, NADP+, H2O in cytosol · endoplasmic reticulum membrane.", "equation": "4CHOL7a,27DONE + NADPH + H+ + O2 → 4CHOL7a,12a,27TONE + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-193835", "reactome_id": "R-ALL-193835", "label": "4CHOL7a,27DONE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48825", "formula": "C27H44O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9617026", "reactome_id": "R-ALL-9617026", "label": "4CHOL7a,12a,27TONE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:48833", "formula": "C27H44O4", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sterol 12-alpha-hydroxylase activity of CYP8B1 [endoplasmic reticulum membrane]", "activity_id": "192125", "ec_numbers": [ "1.14.14.139" ], "accessions": [ "Q9UNU6" ] } ], "citations": [ { "id": "PMID:10051404", "title": "Structure and chromosomal assignment of the sterol 12alpha-hydroxylase gene (CYP8B1) in human and mouse: eukaryotic cytochrome P-450 gene devoid of introns", "journal": "Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10051404/" }, { "id": "PMID:1400444", "title": "Purification and characterization of 7 alpha-hydroxy-4-cholesten-3-one 12 alpha-hydroxylase", "journal": "J. Biol. Chem.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1400444/" } ], "x": 5008.666666666667, "y": 1671.5075757575758, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193961", "label": "Pregn-5-ene-3,20-dione-17-ol isomerizes to 17-hydroxyprogesterone", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193961", "summary": "Pregn-5-ene-3,20-dione-17-ol isomerizes to 17-hydroxyprogesterone is a Reactome release 97 curated reaction event. The source record connects pregn-5-ene-3,20-dione-17-ol to 17aHPROG in cytosol · endoplasmic reticulum membrane.", "equation": "pregn-5-ene-3,20-dione-17-ol → 17aHPROG", "inputs": [ { "node_id": "R-ALL-196366", "reactome_id": "R-ALL-196366", "label": "pregn-5-ene-3,20-dione-17-ol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63843", "formula": "C21H30O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193092", "reactome_id": "R-ALL-193092", "label": "17aHPROG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17252", "formula": "C21H30O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid Delta-isomerase activity of HSD3B1 dimer, HSD3B2 dimer [endoplasmic reticulum membrane]", "activity_id": "193169", "ec_numbers": [ "5.3.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2243100", "title": "Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2243100/" } ], "x": 4451.333333333333, "y": 1736.340909090909, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193964", "label": "CYP21A2 21-hydroxylates PROG", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:50305" ], "source_url": "https://reactome.org/content/detail/R-HSA-193964", "summary": "CYP21A2 21-hydroxylates PROG is a Reactome release 97 curated reaction event. The source record connects P4, NADPH, H+ to 11DCORST, NADP+, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:50305.", "equation": "P4 + NADPH + H+ + O2 → 11DCORST + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-193055", "reactome_id": "R-ALL-193055", "label": "P4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17026", "formula": "C21H30O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194037", "reactome_id": "R-ALL-194037", "label": "11DCORST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16973", "formula": "C21H30O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "progesterone 21-hydroxylase activity of CYP21A2 [endoplasmic reticulum membrane]", "activity_id": "194007", "ec_numbers": null, "accessions": [ "P08686" ] } ], "citations": [ { "id": "PMID:3487786", "title": "Structure of human steroid 21-hydroxylase genes", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3487786/" }, { "id": "PMID:3038528", "title": "Molecular characterization of the HLA-linked steroid 21-hydroxylase B gene from an individual with congenital adrenal hyperplasia", "journal": "EMBO J", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3038528/" } ], "x": 4730, "y": 1583.0984848484848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193965", "label": "CYP11B2 oxidises 18HCORST to ALDO", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:50793" ], "source_url": "https://reactome.org/content/detail/R-HSA-193965", "summary": "CYP11B2 oxidises 18HCORST to ALDO is a Reactome release 97 curated reaction event. The source record connects 18HCORST, NADPH, H+ to ALDO, NADP+, H2O in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:50793.", "equation": "18HCORST + 2 NADPH + 2 H+ + O2 → ALDO + 2 NADP+ + 2 H2O", "inputs": [ { "node_id": "R-ALL-194012", "reactome_id": "R-ALL-194012", "label": "18HCORST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16485", "formula": "C21H30O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193982", "reactome_id": "R-ALL-193982", "label": "ALDO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27584", "formula": "C21H28O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid hydroxylase activity of CYP11B2 [mitochondrial inner membrane]", "activity_id": "193967", "ec_numbers": null, "accessions": [ "P19099" ] } ], "citations": [ { "id": "PMID:2592361", "title": "Characterization of two genes encoding human steroid 11 beta-hydroxylase (P-450(11) beta)", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2592361/" }, { "id": "PMID:2256920", "title": "Cloning and expression of a cDNA for human cytochrome P-450aldo as related to primary aldosteronism", "journal": "Biochem Biophys Res Commun", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2256920/" } ], "x": 5008.666666666667, "y": 1583.0984848484848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193981", "label": "CYP21A2 oxidises 17HPROG", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:50309" ], "source_url": "https://reactome.org/content/detail/R-HSA-193981", "summary": "CYP21A2 oxidises 17HPROG is a Reactome release 97 curated reaction event. The source record connects 17aHPROG, NADPH, H+ to 11-deoxycortisol, NADP+, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:50309.", "equation": "17aHPROG + NADPH + H+ + O2 → 11-deoxycortisol + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-193092", "reactome_id": "R-ALL-193092", "label": "17aHPROG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17252", "formula": "C21H30O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193966", "reactome_id": "R-ALL-193966", "label": "11-deoxycortisol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28324", "formula": "C21H30O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "progesterone 21-hydroxylase activity of CYP21A2 [endoplasmic reticulum membrane]", "activity_id": "194007", "ec_numbers": null, "accessions": [ "P08686" ] } ], "citations": [ { "id": "PMID:3487786", "title": "Structure of human steroid 21-hydroxylase genes", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3487786/" }, { "id": "PMID:3038528", "title": "Molecular characterization of the HLA-linked steroid 21-hydroxylase B gene from an individual with congenital adrenal hyperplasia", "journal": "EMBO J", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3038528/" } ], "x": 4451.333333333333, "y": 1588.9924242424242, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193995", "label": "CYP11B2 18-hydroxylates CORST to 18HCORST", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:11873" ], "source_url": "https://reactome.org/content/detail/R-HSA-193995", "summary": "CYP11B2 18-hydroxylates CORST to 18HCORST is a Reactome release 97 curated reaction event. The source record connects CORST, NADPH, H+ to 18HCORST, NADP+, H2O in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:11873.", "equation": "CORST + 2 NADPH + 2 H+ + O2 → 18HCORST + 2 NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-194005", "reactome_id": "R-ALL-194005", "label": "CORST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16827", "formula": "C21H30O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194012", "reactome_id": "R-ALL-194012", "label": "18HCORST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16485", "formula": "C21H30O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid hydroxylase activity of CYP11B2 [mitochondrial inner membrane]", "activity_id": "193967", "ec_numbers": null, "accessions": [ "P19099" ] } ], "citations": [ { "id": "PMID:2592361", "title": "Characterization of two genes encoding human steroid 11 beta-hydroxylase (P-450(11) beta)", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2592361/" }, { "id": "PMID:2256920", "title": "Cloning and expression of a cDNA for human cytochrome P-450aldo as related to primary aldosteronism", "journal": "Biochem Biophys Res Commun", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2256920/" } ], "x": 4730, "y": 1588.9924242424242, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-193996", "label": "11DCORST translocates from cytosol to mitochondrial matrix", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-193996", "summary": "11DCORST translocates from cytosol to mitochondrial matrix is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 11DCORST to 11DCORST in cytosol · mitochondrial matrix.", "equation": "11DCORST → 11DCORST", "inputs": [ { "node_id": "R-ALL-194037", "reactome_id": "R-ALL-194037", "label": "11DCORST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16973", "formula": "C21H30O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193988", "reactome_id": "R-ALL-193988", "label": "11DCORST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16973", "formula": "C21H30O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15583024", "title": "Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones", "journal": "Endocr Rev", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15583024/" } ], "x": 5008.666666666667, "y": 1848.3257575757575, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-193997", "label": "CYP11B1 oxidises 11DCORT", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:46101" ], "source_url": "https://reactome.org/content/detail/R-HSA-193997", "summary": "CYP11B1 oxidises 11DCORT is a Reactome release 97 curated reaction event. The source record connects 11DCORT, NADPH, H+ to CORT, NADP+, H2O in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:46101.", "equation": "11DCORT + 2 NADPH + 2 H+ + O2 → CORT + 2 NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-193998", "reactome_id": "R-ALL-193998", "label": "11DCORT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28324", "formula": "C21H30O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194011", "reactome_id": "R-ALL-194011", "label": "CORT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17650", "formula": "C21H30O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid 11-beta-monooxygenase activity of Cytochrome P450 (CYP11B1 based) [mitochondrial inner membrane]", "activity_id": "193999", "ec_numbers": [ "1.14.15.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:2592361", "title": "Characterization of two genes encoding human steroid 11 beta-hydroxylase (P-450(11) beta)", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2592361/" }, { "id": "PMID:1741400", "title": "Role of steroid 11 beta-hydroxylase and steroid 18-hydroxylase in the biosynthesis of glucocorticoids and mineralocorticoids in humans", "journal": "Proc Natl Acad Sci U S A", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1741400/" }, { "id": "PMID:9536209", "title": "A critical review of the origin and control of adrenal androgens", "journal": "Baillieres Clin Obstet Gynaecol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9536209/" } ], "x": 5008.666666666667, "y": 1588.9924242424242, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194017", "label": "CYP11B2 oxidises 11DCORST to CORST", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:46105" ], "source_url": "https://reactome.org/content/detail/R-HSA-194017", "summary": "CYP11B2 oxidises 11DCORST to CORST is a Reactome release 97 curated reaction event. The source record connects 11DCORST, NADPH, H+ to CORST, H2O, NADP+ in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:46105.", "equation": "11DCORST + 2 NADPH + 2 H+ + O2 → CORST + H2O + 2 NADP+", "inputs": [ { "node_id": "R-ALL-193988", "reactome_id": "R-ALL-193988", "label": "11DCORST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16973", "formula": "C21H30O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194005", "reactome_id": "R-ALL-194005", "label": "CORST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16827", "formula": "C21H30O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "steroid 11-beta-monooxygenase activity of CYP11B2 [mitochondrial inner membrane]", "activity_id": "5600617", "ec_numbers": [ "1.14.15.4" ], "accessions": [ "P19099" ] } ], "citations": [ { "id": "PMID:2592361", "title": "Characterization of two genes encoding human steroid 11 beta-hydroxylase (P-450(11) beta)", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2592361/" }, { "id": "PMID:1741400", "title": "Role of steroid 11 beta-hydroxylase and steroid 18-hydroxylase in the biosynthesis of glucocorticoids and mineralocorticoids in humans", "journal": "Proc Natl Acad Sci U S A", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1741400/" } ], "x": 4451.333333333333, "y": 1594.8863636363637, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194023", "label": "HSD11B2,HSD11B1 dimer oxidise CORT to COR", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [ "RHEA:68618" ], "source_url": "https://reactome.org/content/detail/R-HSA-194023", "summary": "HSD11B2,HSD11B1 dimer oxidise CORT to COR is a Reactome release 97 curated reaction event. The source record connects CORT, NADP+ to COR, H+, NADPH in endoplasmic reticulum lumen and cross-references RHEA:68618.", "equation": "CORT + NADP+ → COR + H+ + NADPH", "inputs": [ { "node_id": "R-ALL-193977", "reactome_id": "R-ALL-193977", "label": "CORT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17650", "formula": "C21H30O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194035", "reactome_id": "R-ALL-194035", "label": "COR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16962", "formula": "C21H28O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "11-beta-hydroxysteroid dehydrogenase (NAD+) activity of HSD11B2, HSD11B1 dimer [endoplasmic reticulum membrane]", "activity_id": "193976", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:1885595", "title": "The human gene for 11 beta-hydroxysteroid dehydrogenase. Structure, tissue distribution, and chromosomal localization.", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1885595/" }, { "id": "PMID:2889032", "title": "Mineralocorticoid activity of liquorice: 11-beta-hydroxysteroid dehydrogenase deficiency comes of age", "journal": "Lancet", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2889032/" } ], "x": 5008.666666666667, "y": 1559.5227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194025", "label": "11-deoxycortisol translocates to the mitochondrion", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-194025", "summary": "11-deoxycortisol translocates to the mitochondrion is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 11-deoxycortisol to 11DCORT in cytosol · mitochondrial matrix.", "equation": "11-deoxycortisol → 11DCORT", "inputs": [ { "node_id": "R-ALL-193966", "reactome_id": "R-ALL-193966", "label": "11-deoxycortisol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28324", "formula": "C21H30O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193998", "reactome_id": "R-ALL-193998", "label": "11DCORT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28324", "formula": "C21H30O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15583024", "title": "Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones", "journal": "Endocr Rev", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15583024/" } ], "x": 4451.333333333333, "y": 1854.219696969697, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-194036", "label": "Cortisol translocates from the mitochondrial matrix to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "mitochondrial matrix · cytosol", "compartment_ids": [ "GO:0005759", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-194036", "summary": "Cortisol translocates from the mitochondrial matrix to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CORT to CORT in mitochondrial matrix · cytosol.", "equation": "CORT → CORT", "inputs": [ { "node_id": "R-ALL-194011", "reactome_id": "R-ALL-194011", "label": "CORT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17650", "formula": "C21H30O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193977", "reactome_id": "R-ALL-193977", "label": "CORT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17650", "formula": "C21H30O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19202080", "title": "Dynamic regulation of mitochondrial function by glucocorticoids", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19202080/" } ], "x": 4730, "y": 1854.219696969697, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-194079", "label": "SLCO1B3 transports ALB:(GCCA, TCCA) from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:71851", "RHEA:71703", "RHEA:71704", "RHEA:71852" ], "source_url": "https://reactome.org/content/detail/R-HSA-194079", "summary": "SLCO1B3 transports ALB:(GCCA, TCCA) from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects GCCA, TCCA to GCCA, TCCA in plasma membrane and cross-references RHEA:71851, RHEA:71703, RHEA:71704, RHEA:71852.", "equation": "GCCA, TCCA → GCCA, TCCA", "inputs": [ { "node_id": "R-ALL-194117", "reactome_id": "R-ALL-194117", "label": "GCCA, TCCA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194097", "reactome_id": "R-ALL-194097", "label": "GCCA, TCCA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "bile acid transmembrane transporter activity of SLCO1B3 [plasma membrane]", "activity_id": "194088", "ec_numbers": null, "accessions": [ "Q9NPD5" ] } ], "citations": [ { "id": "PMID:11159893", "title": "Organic anion-transporting polypeptide B (OATP-B) and its functional comparison with three other OATPs of human liver", "journal": "Gastroenterology", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11159893/" } ], "x": 5008.666666666667, "y": 1541.840909090909, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-194083", "label": "SLCO1B1 transports ALB:(GCCA,TCCA) from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:71851", "RHEA:71703", "RHEA:71704", "RHEA:71852" ], "source_url": "https://reactome.org/content/detail/R-HSA-194083", "summary": "SLCO1B1 transports ALB:(GCCA,TCCA) from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects GCCA, TCCA to GCCA, TCCA in plasma membrane and cross-references RHEA:71851, RHEA:71703, RHEA:71704, RHEA:71852.", "equation": "GCCA, TCCA → GCCA, TCCA", "inputs": [ { "node_id": "R-ALL-194117", "reactome_id": "R-ALL-194117", "label": "GCCA, TCCA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194097", "reactome_id": "R-ALL-194097", "label": "GCCA, TCCA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "bile acid transmembrane transporter activity of SLCO1B1 [plasma membrane]", "activity_id": "194120", "ec_numbers": null, "accessions": [ "Q9Y6L6" ] } ], "citations": [ { "id": "PMID:10358072", "title": "Identification of a novel gene family encoding human liver-specific organic anion transporter LST-1", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10358072/" }, { "id": "PMID:10601278", "title": "A novel human hepatic organic anion transporting polypeptide (OATP2). Identification of a liver-specific human organic anion transporting polypeptide and identification of rat and human hydroxymethylglutaryl-CoA reductase inhibitor transporters", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10601278/" }, { "id": "PMID:11159893", "title": "Organic anion-transporting polypeptide B (OATP-B) and its functional comparison with three other OATPs of human liver", "journal": "Gastroenterology", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11159893/" } ], "x": 4451.333333333333, "y": 1547.7348484848485, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-194121", "label": "SLC10A1 co-transport bile salts and Na+ from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-194121", "summary": "SLC10A1 co-transport bile salts and Na+ from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects Na+, bile salts and acids to Na+, bile salts and acids in plasma membrane.", "equation": "2 Na+ + bile salts and acids → 2 Na+ + bile salts and acids", "inputs": [ { "node_id": "R-ALL-74113", "reactome_id": "R-ALL-74113", "label": "Na+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192483", "reactome_id": "R-ALL-192483", "label": "bile salts and acids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-83910", "reactome_id": "R-ALL-83910", "label": "Na+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194156", "reactome_id": "R-ALL-194156", "label": "bile salts and acids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "bile acid:sodium symporter activity of SLC10A1 [plasma membrane]", "activity_id": "194135", "ec_numbers": null, "accessions": [ "Q14973" ] } ], "citations": [ { "id": "PMID:8132774", "title": "Molecular cloning, chromosomal localization, and functional characterization of a human liver Na+/bile acid cotransporter", "journal": "J Clin Invest", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8132774/" } ], "x": 4730, "y": 1736.340909090909, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-194130", "label": "Transport (influx) of bile salts and acids by OATP-A", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:75051", "RHEA:71703", "RHEA:72683", "RHEA:71851", "RHEA:71855", "RHEA:71859", "RHEA:71704", "RHEA:71852", "RHEA:71856", "RHEA:71860", "RHEA:72684", "RHEA:75052" ], "source_url": "https://reactome.org/content/detail/R-HSA-194130", "summary": "Transport (influx) of bile salts and acids by OATP-A is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects bile salts and acids to bile salts and acids in plasma membrane and cross-references RHEA:75051, RHEA:71703, RHEA:72683, RHEA:71851, RHEA:71855, RHEA:71859, RHEA:71704, RHEA:71852, RHEA:71856, RHEA:71860, RHEA:72684, RHEA:75052.", "equation": "bile salts and acids → bile salts and acids", "inputs": [ { "node_id": "R-ALL-192483", "reactome_id": "R-ALL-192483", "label": "bile salts and acids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194156", "reactome_id": "R-ALL-194156", "label": "bile salts and acids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "bile acid transmembrane transporter activity of SLCO1A2 [plasma membrane]", "activity_id": "194128", "ec_numbers": null, "accessions": [ "P46721" ] } ], "citations": [ { "id": "PMID:7557095", "title": "Molecular and functional characterization of an organic anion transporting polypeptide cloned from human liver", "journal": "Gastroenterology", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7557095/" } ], "x": 5391.333333333333, "y": 1727.5, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-194153", "label": "ABCC3 transports bile salts from cytosol to extracellular region", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane · cytosol · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005829", "GO:0005576" ], "rhea_ids": [ "RHEA:50056", "RHEA:50052", "RHEA:50060", "RHEA:50064", "RHEA:50048" ], "source_url": "https://reactome.org/content/detail/R-HSA-194153", "summary": "ABCC3 transports bile salts from cytosol to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects bile salts and acids, ATP to ADP, Pi, bile salts and acids in plasma membrane · cytosol · extracellular region and cross-references RHEA:50056, RHEA:50052, RHEA:50060, RHEA:50064, RHEA:50048.", "equation": "bile salts and acids + ATP → ADP + Pi + bile salts and acids", "inputs": [ { "node_id": "R-ALL-194156", "reactome_id": "R-ALL-194156", "label": "bile salts and acids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192483", "reactome_id": "R-ALL-192483", "label": "bile salts and acids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "ABC-type bile acid transporter activity of ABCC3 [plasma membrane]", "activity_id": "194154", "ec_numbers": null, "accessions": [ "O15438" ] } ], "citations": [ { "id": "PMID:12704183", "title": "Differential modulation of the human liver conjugate transporters MRP2 and MRP3 by bile acids and organic anions", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12704183/" }, { "id": "PMID:10987286", "title": "Transport of amphipathic anions by human multidrug resistance protein 3", "journal": "Cancer Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10987286/" }, { "id": "PMID:12220224", "title": "Transport of bile acids in multidrug-resistance-protein 3-overexpressing cells co-transfected with the ileal Na+-dependent bile-acid transporter", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12220224/" } ], "x": 5008.666666666667, "y": 1535.9469696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-194187", "label": "SLC10A2 transports bile salts and acids and Na+ from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-194187", "summary": "SLC10A2 transports bile salts and acids and Na+ from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects Na+, bile salts and acids to bile salts and acids, Na+ in plasma membrane.", "equation": "Na+ + bile salts and acids → bile salts and acids + Na+", "inputs": [ { "node_id": "R-ALL-74113", "reactome_id": "R-ALL-74113", "label": "Na+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192483", "reactome_id": "R-ALL-192483", "label": "bile salts and acids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194156", "reactome_id": "R-ALL-194156", "label": "bile salts and acids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-83910", "reactome_id": "R-ALL-83910", "label": "Na+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "bile acid:sodium symporter activity of SLC10A2 [plasma membrane]", "activity_id": "194178", "ec_numbers": null, "accessions": [ "Q12908" ] } ], "citations": [ { "id": "PMID:12663868", "title": "Bile salt transporters: molecular characterization, function, and regulation", "journal": "Physiol Rev", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12663868/" }, { "id": "PMID:14699511", "title": "Enterohepatic bile salt transporters in normal physiology and liver disease", "journal": "Gastroenterology", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14699511/" }, { "id": "PMID:12486725", "title": "Insights into the bile acid transportation system: the human ileal lipid-binding protein-cholyltaurine complex and its comparison with homologous structures", "journal": "Proteins", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12486725/" } ], "x": 5008.666666666667, "y": 1736.340909090909, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-194632", "label": "Zymosterone (cholesta-8(9),24-dien-3-one) is reduced to zymosterol (cholesta-8(9),24-dien-3beta-ol)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:33459", "RHEA:33460" ], "source_url": "https://reactome.org/content/detail/R-HSA-194632", "summary": "Zymosterone (cholesta-8(9),24-dien-3-one) is reduced to zymosterol (cholesta-8(9),24-dien-3beta-ol) is a Reactome release 97 curated reaction event. The source record connects ZYMONE, NADPH, H+ to ZYMOL, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:33459, RHEA:33460.", "equation": "ZYMONE + NADPH + H+ → ZYMOL + NADP+", "inputs": [ { "node_id": "R-ALL-194661", "reactome_id": "R-ALL-194661", "label": "ZYMONE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:52386", "formula": "C27H42O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194691", "reactome_id": "R-ALL-194691", "label": "ZYMOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18252", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity of HSD17B7 [endoplasmic reticulum membrane]", "activity_id": "194731", "ec_numbers": [ "1.1.1.270" ], "accessions": [ "P56937" ] } ], "citations": [ { "id": "PMID:12829805", "title": "Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis", "journal": "Mol. Endocrinol.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12829805/" } ], "x": 2381.3333333333335, "y": 2076.8333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194641", "label": "4,4-dimethylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:55245" ], "source_url": "https://reactome.org/content/detail/R-HSA-194641", "summary": "4,4-dimethylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol is a Reactome release 97 curated reaction event. The source record connects H+, NADPH, O2 to 4C4MZYMOL, H2O, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:55245.", "equation": "3 H+ + 3 NADPH + 3 O2 + 14DMLANOL → 4C4MZYMOL + 4 H2O + 3 NADP+", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194633", "reactome_id": "R-ALL-194633", "label": "14DMLANOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18364", "formula": "C29H48O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194735", "reactome_id": "R-ALL-194735", "label": "4C4MZYMOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64925", "formula": "C29H45O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "C-4 methylsterol oxidase activity of MSMO1 [endoplasmic reticulum membrane]", "activity_id": "194699", "ec_numbers": [ "1.14.18.9" ], "accessions": [ "Q15800" ] } ], "citations": [ { "id": "PMID:8663358", "title": "Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8663358/" } ], "x": 2660, "y": 2310.2333333333336, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194642", "label": "4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form 4-methylcholesta-8(9),24-dien-3-one", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:33448" ], "source_url": "https://reactome.org/content/detail/R-HSA-194642", "summary": "4-methyl,4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form 4-methylcholesta-8(9),24-dien-3-one is a Reactome release 97 curated reaction event. The source record connects 4C4MZYMOL, NADP+ to dh4MZYMOL, H+, CO2 in cytosol · endoplasmic reticulum membrane and cross-references RHEA:33448.", "equation": "4C4MZYMOL + NADP+ → dh4MZYMOL + H+ + CO2 + NADPH", "inputs": [ { "node_id": "R-ALL-194735", "reactome_id": "R-ALL-194735", "label": "4C4MZYMOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64925", "formula": "C29H45O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194667", "reactome_id": "R-ALL-194667", "label": "dh4MZYMOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:50593", "formula": "C28H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity of NSDHL [endoplasmic reticulum membrane]", "activity_id": "194724", "ec_numbers": [ "1.1.1.170" ], "accessions": [ "Q15738" ] } ], "citations": [ { "id": "PMID:14506130", "title": "NSDHL, an enzyme involved in cholesterol biosynthesis, traffics through the Golgi and accumulates on ER membranes and on the surface of lipid droplets", "journal": "Hum Mol Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14506130/" } ], "x": 2381.3333333333335, "y": 2336.1666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194669", "label": "4-methylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-carboxycholesta-8(9),24-dien-3beta-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:47057" ], "source_url": "https://reactome.org/content/detail/R-HSA-194669", "summary": "4-methylcholesta-8(9),24-dien-3beta-ol is oxidized to 4-carboxycholesta-8(9),24-dien-3beta-ol is a Reactome release 97 curated reaction event. The source record connects 4MZYMOL, H+, NADPH to 4CZYMOL, H2O, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:47057.", "equation": "4MZYMOL + 3 H+ + 3 NADPH + 3 O2 → 4CZYMOL + 4 H2O + 3 NADP+", "inputs": [ { "node_id": "R-ALL-194723", "reactome_id": "R-ALL-194723", "label": "4MZYMOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:1949", "formula": "C28H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194657", "reactome_id": "R-ALL-194657", "label": "4CZYMOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:143575", "formula": "C28H43O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "C-4 methylsterol oxidase activity of MSMO1 [endoplasmic reticulum membrane]", "activity_id": "194699", "ec_numbers": [ "1.14.18.9" ], "accessions": [ "Q15800" ] } ], "citations": [ { "id": "PMID:8663358", "title": "Characterization of yeast methyl sterol oxidase (ERG25) and identification of a human homologue", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8663358/" } ], "x": 2938.666666666667, "y": 2310.2333333333336, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194674", "label": "4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol is reduced to 4,4-dimethylcholesta-8(9),24-dien-3beta-ol [LBR]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "nuclear envelope", "compartment_ids": [ "GO:0005635" ], "rhea_ids": [ "RHEA:18563" ], "source_url": "https://reactome.org/content/detail/R-HSA-194674", "summary": "4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol is reduced to 4,4-dimethylcholesta-8(9),24-dien-3beta-ol [LBR] is a Reactome release 97 curated reaction event. The source record connects 4,4DMCHtOL, NADPH, H+ to 14DMLANOL, NADP+ in nuclear envelope and cross-references RHEA:18563.", "equation": "4,4DMCHtOL + NADPH + H+ → 14DMLANOL + NADP+", "inputs": [ { "node_id": "R-ALL-194681", "reactome_id": "R-ALL-194681", "label": "4,4DMCHtOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17813", "formula": "C29H46O", "compartment": "nuclear envelope", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194725", "reactome_id": "R-ALL-194725", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "nuclear envelope", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-194688", "reactome_id": "R-ALL-194688", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nuclear envelope", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194704", "reactome_id": "R-ALL-194704", "label": "14DMLANOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18364", "formula": "C29H48O", "compartment": "nuclear envelope", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194668", "reactome_id": "R-ALL-194668", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "nuclear envelope", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "Delta14-sterol reductase activity of LBR [nuclear envelope]", "activity_id": "194706", "ec_numbers": [ "1.3.1.70" ], "accessions": [ "Q14739" ] } ], "citations": [ { "id": "PMID:12618959", "title": "Autosomal recessive HEM/Greenberg skeletal dysplasia is caused by 3 beta-hydroxysterol delta 14-reductase deficiency due to mutations in the lamin B receptor gene", "journal": "Am J Hum Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12618959/" } ], "x": 2660, "y": 2076.8333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194678", "label": "CYP51A1 demethylates LAN", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "biological-oxidations", "cholesterol", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:25287" ], "source_url": "https://reactome.org/content/detail/R-HSA-194678", "summary": "CYP51A1 demethylates LAN is a Reactome release 97 curated reaction event. The source record connects LAN, H+, NADPH to 4,4DMCHtOL, HCOOH, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:25287.", "equation": "LAN + 3 H+ + 3 NADPH + 3 O2 → 4,4DMCHtOL + HCOOH + 4 H2O + 3 NADP+", "inputs": [ { "node_id": "R-ALL-194634", "reactome_id": "R-ALL-194634", "label": "LAN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16521", "formula": "C30H50O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194644", "reactome_id": "R-ALL-194644", "label": "4,4DMCHtOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17813", "formula": "C29H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom…", "activity_id": "194693", "ec_numbers": [ "1.14.14.1" ], "accessions": [ "Q16850" ] } ], "citations": [ { "id": "PMID:8619637", "title": "The ubiquitously expressed human CYP51 encodes lanosterol 14 alpha-demethylase, a cytochrome P450 whose expression is regulated by oxysterols", "journal": "Arch Biochem Biophys", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8619637/" }, { "id": "PMID:20149798", "title": "Structural basis of human CYP51 inhibition by antifungal azoles", "journal": "J. Mol. Biol.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20149798/" } ], "x": 2938.666666666667, "y": 2284.3, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194689", "label": "4-methylcholesta-8(9),24-dien-3-one is reduced to 4-methylcholesta-8(9),24-dien-3beta-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:36381" ], "source_url": "https://reactome.org/content/detail/R-HSA-194689", "summary": "4-methylcholesta-8(9),24-dien-3-one is reduced to 4-methylcholesta-8(9),24-dien-3beta-ol is a Reactome release 97 curated reaction event. The source record connects dh4MZYMOL, NADPH, H+ to 4MZYMOL, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:36381.", "equation": "dh4MZYMOL + NADPH + H+ → 4MZYMOL + NADP+", "inputs": [ { "node_id": "R-ALL-194667", "reactome_id": "R-ALL-194667", "label": "dh4MZYMOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:50593", "formula": "C28H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194723", "reactome_id": "R-ALL-194723", "label": "4MZYMOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:1949", "formula": "C28H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity of HSD17B7 [endoplasmic reticulum membrane]", "activity_id": "194731", "ec_numbers": [ "1.1.1.270" ], "accessions": [ "P56937" ] } ], "citations": [ { "id": "PMID:12829805", "title": "Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis", "journal": "Mol. Endocrinol.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12829805/" } ], "x": 2381.3333333333335, "y": 2362.1, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194698", "label": "4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol is reduced to 4,4-dimethylcholesta-8(9),24-dien-3beta-ol [TM7SF2]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:18561", "RHEA:18563" ], "source_url": "https://reactome.org/content/detail/R-HSA-194698", "summary": "4,4-dimethylcholesta-8(9),14,24-trien-3beta-ol is reduced to 4,4-dimethylcholesta-8(9),24-dien-3beta-ol [TM7SF2] is a Reactome release 97 curated reaction event. The source record connects 4,4DMCHtOL, NADPH, H+ to 14DMLANOL, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:18561, RHEA:18563.", "equation": "4,4DMCHtOL + NADPH + H+ → 14DMLANOL + NADP+", "inputs": [ { "node_id": "R-ALL-194644", "reactome_id": "R-ALL-194644", "label": "4,4DMCHtOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17813", "formula": "C29H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194633", "reactome_id": "R-ALL-194633", "label": "14DMLANOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18364", "formula": "C29H48O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "Delta14-sterol reductase activity of TM7SF2 [endoplasmic reticulum membrane]", "activity_id": "194714", "ec_numbers": [ "1.3.1.70" ], "accessions": [ "O76062" ] } ], "citations": [ { "id": "PMID:16784888", "title": "Sterol dependent regulation of human TM7SF2 gene expression: role of the encoded 3beta-hydroxysterol Delta14-reductase in human cholesterol biosynthesis", "journal": "Biochim Biophys Acta", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16784888/" } ], "x": 2938.666666666667, "y": 2076.8333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-194718", "label": "4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form cholesta-8(9),24-dien-3-one (zymosterone)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:33455" ], "source_url": "https://reactome.org/content/detail/R-HSA-194718", "summary": "4-carboxycholesta-8(9),24-dien-3beta-ol is decarboxylated and oxidized to form cholesta-8(9),24-dien-3-one (zymosterone) is a Reactome release 97 curated reaction event. The source record connects 4CZYMOL, NAD+ to ZYMONE, CO2, H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:33455.", "equation": "4CZYMOL + NAD+ → ZYMONE + CO2 + H+ + NADH", "inputs": [ { "node_id": "R-ALL-194657", "reactome_id": "R-ALL-194657", "label": "4CZYMOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:143575", "formula": "C28H43O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-194661", "reactome_id": "R-ALL-194661", "label": "ZYMONE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:52386", "formula": "C27H42O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity of NSDHL [endoplasmic reticulum membrane]", "activity_id": "194724", "ec_numbers": [ "1.1.1.170" ], "accessions": [ "Q15738" ] } ], "citations": [ { "id": "PMID:14506130", "title": "NSDHL, an enzyme involved in cholesterol biosynthesis, traffics through the Golgi and accumulates on ER membranes and on the surface of lipid droplets", "journal": "Hum Mol Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14506130/" } ], "x": 2660, "y": 2336.1666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-195664", "label": "Cholesta-7,24-dien-3beta-ol is desaturated to form cholesta-5,7,24-trien-3beta-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:34003", "RHEA:34004" ], "source_url": "https://reactome.org/content/detail/R-HSA-195664", "summary": "Cholesta-7,24-dien-3beta-ol is desaturated to form cholesta-5,7,24-trien-3beta-ol is a Reactome release 97 curated reaction event. The source record connects CHdOL, NADPH, H+ to 7dhDESOL, NADP+, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:34003, RHEA:34004.", "equation": "CHdOL + NADPH + H+ + O2 → 7dhDESOL + NADP+ + 2 H2O", "inputs": [ { "node_id": "R-ALL-195691", "reactome_id": "R-ALL-195691", "label": "CHdOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16290", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-195655", "reactome_id": "R-ALL-195655", "label": "7dhDESOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27910", "formula": "C27H42O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "C-5 sterol desaturase activity of SC5D [endoplasmic reticulum membrane]", "activity_id": "195666", "ec_numbers": null, "accessions": [ "O75845" ] } ], "citations": [ { "id": "PMID:12189593", "title": "Lathosterolosis, a novel multiple-malformation/mental retardation syndrome due to deficiency of 3beta-hydroxysteroid-delta5-desaturase", "journal": "Am J Hum Genet", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12189593/" }, { "id": "PMID:12812989", "title": "Lathosterolosis: an inborn error of human and murine cholesterol synthesis due to lathosterol 5-desaturase deficiency", "journal": "Hum Mol Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12812989/" } ], "x": 2381.3333333333335, "y": 2024.9666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-195690", "label": "Zymosterol is isomerized to cholesta-7,24-dien-3beta-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [ "RHEA:34000" ], "source_url": "https://reactome.org/content/detail/R-HSA-195690", "summary": "Zymosterol is isomerized to cholesta-7,24-dien-3beta-ol is a Reactome release 97 curated reaction event. The source record connects ZYMOL to CHdOL in endoplasmic reticulum membrane and cross-references RHEA:34000.", "equation": "ZYMOL → CHdOL", "inputs": [ { "node_id": "R-ALL-194691", "reactome_id": "R-ALL-194691", "label": "ZYMOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18252", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-195691", "reactome_id": "R-ALL-195691", "label": "CHdOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16290", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid Delta-isomerase activity of EBP [endoplasmic reticulum membrane]", "activity_id": "195674", "ec_numbers": [ "5.3.3.1" ], "accessions": [ "Q15125" ] } ], "citations": [ { "id": "PMID:10391218", "title": "Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com.", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10391218/" }, { "id": "PMID:10391219", "title": "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hunermann syndrome", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10391219/" } ], "x": 2938.666666666667, "y": 2258.366666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196060", "label": "Reduction of isocaproaldehyde to 4-methylpentan-1-ol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:58438" ], "source_url": "https://reactome.org/content/detail/R-HSA-196060", "summary": "Reduction of isocaproaldehyde to 4-methylpentan-1-ol is a Reactome release 97 curated reaction event. The source record connects H+, ISCAL, NADPH to MePeOH, NADP+ in cytosol and cross-references RHEA:58438.", "equation": "H+ + ISCAL + NADPH → MePeOH + NADP+", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-196095", "reactome_id": "R-ALL-196095", "label": "ISCAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17998", "formula": "C6H12O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196066", "reactome_id": "R-ALL-196066", "label": "MePeOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63910", "formula": "C6H14O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldose reductase (NADPH) activity of AKR1B1 [cytosol]", "activity_id": "196106", "ec_numbers": [ "1.1.1.21" ], "accessions": [ "P15121" ] } ], "citations": [ { "id": "PMID:8645003", "title": "Aldose reductase is a major reductase for isocaproaldehyde, a product of side-chain cleavage of cholesterol, in human and animal adrenal glands", "journal": "Arch Biochem Biophys", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8645003/" } ], "x": 4451.333333333333, "y": 1606.6742424242425, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196125", "label": "Translocation of isocaproaldehyde from the mitochondrial matrix to the cytosol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-196125", "summary": "Translocation of isocaproaldehyde from the mitochondrial matrix to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ISCAL to ISCAL in mitochondrial inner membrane.", "equation": "ISCAL → ISCAL", "inputs": [ { "node_id": "R-ALL-193046", "reactome_id": "R-ALL-193046", "label": "ISCAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17998", "formula": "C6H12O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-196095", "reactome_id": "R-ALL-196095", "label": "ISCAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17998", "formula": "C6H12O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [], "x": 4451.333333333333, "y": 1824.75, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-196126", "label": "STAR mediates CHOL translocation from cytosol to mitochondrial intermembrane space", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "mitochondrial outer membrane · cytosol · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005741", "GO:0005829", "GO:0005758" ], "rhea_ids": [ "RHEA:39747" ], "source_url": "https://reactome.org/content/detail/R-HSA-196126", "summary": "STAR mediates CHOL translocation from cytosol to mitochondrial intermembrane space is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects STARD3:STARD3NL, STARD4,6:CHOL to STARD3:STARD3NL, STARD4,6, CHOL in mitochondrial outer membrane · cytosol · mitochondrial intermembrane space and cross-references RHEA:39747.", "equation": "STARD3:STARD3NL, STARD4,6:CHOL → STARD3:STARD3NL, STARD4,6 + CHOL", "inputs": [ { "node_id": "R-HSA-196124", "reactome_id": "R-HSA-196124", "label": "STARD3:STARD3NL, STARD4,6:CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-196094", "reactome_id": "R-HSA-196094", "label": "STARD3:STARD3NL, STARD4,6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "CandidateSet", "currency": false }, { "node_id": "R-ALL-193156", "reactome_id": "R-ALL-193156", "label": "CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of STAR [mitochondrial intermembrane space]", "activity_id": "9954142", "ec_numbers": null, "accessions": [ "P49675" ] } ], "citations": [ { "id": "PMID:25542846", "title": "The binding site specificity of STARD4 subfamily: Breaking the cholesterol paradigm", "journal": "Mol. Cell. Endocrinol.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25542846/" } ], "x": 5008.666666666667, "y": 1630.25, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-196350", "label": "Pregnenolone is dehydrogenated to form pregn-5-ene-3,20-dione", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:43925" ], "source_url": "https://reactome.org/content/detail/R-HSA-196350", "summary": "Pregnenolone is dehydrogenated to form pregn-5-ene-3,20-dione is a Reactome release 97 curated reaction event. The source record connects PREG, NAD+ to pregn-5-ene-3,20-dione, NADH, H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:43925.", "equation": "PREG + NAD+ → pregn-5-ene-3,20-dione + NADH + H+", "inputs": [ { "node_id": "R-ALL-193103", "reactome_id": "R-ALL-193103", "label": "PREG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16581", "formula": "C21H32O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196344", "reactome_id": "R-ALL-196344", "label": "pregn-5-ene-3,20-dione", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63837", "formula": "C21H30O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity of HSD3B1 dimer, HSD3B2 dimer [endoplasmic reticulum membrane]", "activity_id": "196330", "ec_numbers": [ "1.1.1.145" ], "accessions": [] } ], "citations": [ { "id": "PMID:2243100", "title": "Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2243100/" }, { "id": "PMID:2770297", "title": "Human placental 3 beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5----4-ene-isomerase: purification from mitochondria and kinetic profiles, biophysical characterization of the purified mitochondrial and microsomal enzymes", "journal": "J Steroid Biochem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2770297/" }, { "id": "PMID:1944309", "title": "Structure and expression of a new complementary DNA encoding the almost exclusive 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase in human adrenals and gonads", "journal": "Mol Endocrinol", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1944309/" }, { "id": "PMID:12832414", "title": "Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12832414/" } ], "x": 4730, "y": 1606.6742424242425, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196372", "label": "17-Hydroxypregnenolone is dehydrogenated to form pregn-5-ene-3,20-dione-17-ol", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-196372", "summary": "17-Hydroxypregnenolone is dehydrogenated to form pregn-5-ene-3,20-dione-17-ol is a Reactome release 97 curated reaction event. The source record connects 17aHPREG, NAD+ to pregn-5-ene-3,20-dione-17-ol, NADH, H+ in cytosol · endoplasmic reticulum membrane.", "equation": "17aHPREG + NAD+ → pregn-5-ene-3,20-dione-17-ol + NADH + H+", "inputs": [ { "node_id": "R-ALL-193124", "reactome_id": "R-ALL-193124", "label": "17aHPREG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28750", "formula": "C21H32O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196366", "reactome_id": "R-ALL-196366", "label": "pregn-5-ene-3,20-dione-17-ol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63843", "formula": "C21H30O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxy-Delta5-steroid dehydrogenase (NAD+) activity of HSD3B1 dimer, HSD3B2 dimer [endoplasmic reticulum membrane]", "activity_id": "196330", "ec_numbers": [ "1.1.1.145" ], "accessions": [] } ], "citations": [ { "id": "PMID:2243100", "title": "Characterization of human 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase gene and its expression in mammalian cells", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2243100/" }, { "id": "PMID:2770297", "title": "Human placental 3 beta-hydroxy-5-ene-steroid dehydrogenase and steroid 5----4-ene-isomerase: purification from mitochondria and kinetic profiles, biophysical characterization of the purified mitochondrial and microsomal enzymes", "journal": "J Steroid Biochem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2770297/" }, { "id": "PMID:1944309", "title": "Structure and expression of a new complementary DNA encoding the almost exclusive 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase in human adrenals and gonads", "journal": "Mol Endocrinol", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1944309/" }, { "id": "PMID:12832414", "title": "Structure/function relationships responsible for coenzyme specificity and the isomerase activity of human type 1 3 beta-hydroxysteroid dehydrogenase/isomerase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12832414/" } ], "x": 4451.333333333333, "y": 1712.7651515151515, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196402", "label": "Cholesta-5,7,24-trien-3beta-ol is reduced to desmosterol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:46740", "RHEA:46741" ], "source_url": "https://reactome.org/content/detail/R-HSA-196402", "summary": "Cholesta-5,7,24-trien-3beta-ol is reduced to desmosterol is a Reactome release 97 curated reaction event. The source record connects 7dhDESOL, NADPH, H+ to DESMOL, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:46740, RHEA:46741.", "equation": "7dhDESOL + NADPH + H+ → DESMOL + NADP+", "inputs": [ { "node_id": "R-ALL-195655", "reactome_id": "R-ALL-195655", "label": "7dhDESOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27910", "formula": "C27H42O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196420", "reactome_id": "R-ALL-196420", "label": "DESMOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17737", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "7-dehydrocholesterol reductase activity of DHCR7 [endoplasmic reticulum membrane]", "activity_id": "196403", "ec_numbers": [ "1.3.1.21" ], "accessions": [ "Q9UBM7" ] } ], "citations": [ { "id": "PMID:9465114", "title": "Molecular cloning and expression of the human delta7-sterol reductase", "journal": "Proc Natl Acad Sci U S A", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9465114/" } ], "x": 2381.3333333333335, "y": 2102.766666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196417", "label": "Reduction of desmosterol to cholesterol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:36391", "RHEA:36393" ], "source_url": "https://reactome.org/content/detail/R-HSA-196417", "summary": "Reduction of desmosterol to cholesterol is a Reactome release 97 curated reaction event. The source record connects DESMOL, NADPH, H+ to CHOL, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:36391, RHEA:36393.", "equation": "DESMOL + NADPH + H+ → CHOL + NADP+", "inputs": [ { "node_id": "R-ALL-196420", "reactome_id": "R-ALL-196420", "label": "DESMOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17737", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193384", "reactome_id": "R-ALL-193384", "label": "CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "Delta24-sterol reductase activity of DHCR24 [endoplasmic reticulum membrane]", "activity_id": "196404", "ec_numbers": [ "1.3.1.72" ], "accessions": [ "Q15392" ] } ], "citations": [ { "id": "PMID:11519011", "title": "Mutations in the 3beta-hydroxysterol Delta24-reductase gene cause desmosterolosis, an autosomal recessive disorder of cholesterol biosynthesis", "journal": "Am J Hum Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11519011/" } ], "x": 2660, "y": 2102.766666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196753", "label": "2xPPCS ligates PPanK with Cys", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25157" ], "source_url": "https://reactome.org/content/detail/R-HSA-196753", "summary": "2xPPCS ligates PPanK with Cys is a Reactome release 97 curated reaction event. The source record connects PPanK, L-Cys, ATP to PPC, AMP, PPi in cytosol and cross-references RHEA:25157.", "equation": "PPanK + L-Cys + ATP → PPC + AMP + PPi + H+", "inputs": [ { "node_id": "R-ALL-196786", "reactome_id": "R-ALL-196786", "label": "PPanK", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:10986", "formula": "C9H15NO8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196834", "reactome_id": "R-ALL-196834", "label": "PPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59458", "formula": "C12H20N2O9PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphopantothenate--cysteine ligase activity of 2xPPCS [cytosol]", "activity_id": "196734", "ec_numbers": [ "6.3.2.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:11923312", "title": "Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11923312/" }, { "id": "PMID:12906824", "title": "Structure of human phosphopantothenoylcysteine synthetase at 2.3 A resolution.", "journal": "Structure", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12906824/" } ], "x": 5391.333333333333, "y": 2890.0131578947367, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196754", "label": "COASY transfers an adenylyl group from ATP to PPANT", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:19802" ], "source_url": "https://reactome.org/content/detail/R-HSA-196754", "summary": "COASY transfers an adenylyl group from ATP to PPANT is a Reactome release 97 curated reaction event. The source record connects ATP, H+, PPANT to DP-CoA, PPi in cytosol · mitochondrial outer membrane and cross-references RHEA:19802.", "equation": "ATP + H+ + PPANT → DP-CoA + PPi", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-196767", "reactome_id": "R-ALL-196767", "label": "PPANT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61723", "formula": "C11H21N2O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-196778", "reactome_id": "R-ALL-196778", "label": "DP-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57328", "formula": "C21H33N7O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pantetheine-phosphate adenylyltransferase activity of COASY [mitochondrial outer membrane]", "activity_id": "196830", "ec_numbers": [ "2.7.7.3" ], "accessions": [ "Q13057" ] } ], "citations": [ { "id": "PMID:11994049", "title": "Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase)", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11994049/" }, { "id": "PMID:14514684", "title": "Subcellular localization and regulation of coenzyme A synthase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14514684/" } ], "x": 5391.333333333333, "y": 2910.4868421052633, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196761", "label": "2xTPK1:Mg2+ phosphorylates THMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11577" ], "source_url": "https://reactome.org/content/detail/R-HSA-196761", "summary": "2xTPK1:Mg2+ phosphorylates THMN is a Reactome release 97 curated reaction event. The source record connects THMN, ATP to ThDP, AMP in cytosol and cross-references RHEA:11577.", "equation": "THMN + ATP → ThDP + AMP", "inputs": [ { "node_id": "R-ALL-196934", "reactome_id": "R-ALL-196934", "label": "THMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18385", "formula": "C12H17N4OS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29480", "reactome_id": "R-ALL-29480", "label": "ThDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58937", "formula": "C12H16N4O7P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "thiamine diphosphokinase activity of 2xTPK1:Mg2+ [cytosol]", "activity_id": "196957", "ec_numbers": [ "2.7.6.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11342111", "title": "Isolation and characterization of a human thiamine pyrophosphokinase cDNA", "journal": "Biochim Biophys Acta", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11342111/" }, { "id": "PMID:11342117", "title": "Molecular cloning of human thiamin pyrophosphokinase", "journal": "Biochim Biophys Acta", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11342117/" } ], "x": 5670, "y": 2951.434210526316, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196773", "label": "COASY phosphorylates DP-CoA", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:18246" ], "source_url": "https://reactome.org/content/detail/R-HSA-196773", "summary": "COASY phosphorylates DP-CoA is a Reactome release 97 curated reaction event. The source record connects ATP, DP-CoA to ADP, CoA-SH, H+ in cytosol · mitochondrial outer membrane and cross-references RHEA:18246.", "equation": "ATP + DP-CoA → ADP + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-196778", "reactome_id": "R-ALL-196778", "label": "DP-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57328", "formula": "C21H33N7O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dephospho-CoA kinase activity of COASY [mitochondrial outer membrane]", "activity_id": "196818", "ec_numbers": [ "2.7.1.24" ], "accessions": [ "Q13057" ] } ], "citations": [ { "id": "PMID:11994049", "title": "Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase)", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11994049/" }, { "id": "PMID:14514684", "title": "Subcellular localization and regulation of coenzyme A synthase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14514684/" } ], "x": 5670, "y": 2910.4868421052633, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196840", "label": "3xPPCDC:3FMN decarboxylates PPC", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16794" ], "source_url": "https://reactome.org/content/detail/R-HSA-196840", "summary": "3xPPCDC:3FMN decarboxylates PPC is a Reactome release 97 curated reaction event. The source record connects PPC, H+ to PPANT, CO2 in cytosol and cross-references RHEA:16794.", "equation": "PPC + H+ → PPANT + CO2", "inputs": [ { "node_id": "R-ALL-196834", "reactome_id": "R-ALL-196834", "label": "PPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59458", "formula": "C12H20N2O9PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196767", "reactome_id": "R-ALL-196767", "label": "PPANT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61723", "formula": "C11H21N2O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphopantothenoylcysteine decarboxylase activity of 3xPPCDC:3xFMN [cytosol]", "activity_id": "196842", "ec_numbers": [ "4.1.1.36" ], "accessions": [] } ], "citations": [ { "id": "PMID:11923312", "title": "Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11923312/" }, { "id": "PMID:15581364", "title": "Mechanistic studies on phosphopantothenoylcysteine decarboxylase: trapping of an enethiolate intermediate with a mechanism-based inactivating agent", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15581364/" } ], "x": 5948.666666666667, "y": 2951.434210526316, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196857", "label": "PANK2 phosphorylates PanK", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005829", "GO:0005758" ], "rhea_ids": [ "RHEA:16374" ], "source_url": "https://reactome.org/content/detail/R-HSA-196857", "summary": "PANK2 phosphorylates PanK is a Reactome release 97 curated reaction event. The source record connects PanK, ATP to PPanK, ADP, H+ in cytosol · mitochondrial intermembrane space and cross-references RHEA:16374.", "equation": "PanK + ATP → PPanK + ADP + H+", "inputs": [ { "node_id": "R-ALL-196820", "reactome_id": "R-ALL-196820", "label": "PanK", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29032", "formula": "C9H16NO5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196786", "reactome_id": "R-ALL-196786", "label": "PPanK", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:10986", "formula": "C9H15NO8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pantothenate kinase activity of PANK2(141-570) [mitochondrial intermembrane space]", "activity_id": "196799", "ec_numbers": [ "2.7.1.33" ], "accessions": [ "Q9BZ23" ] } ], "citations": [ { "id": "PMID:12554685", "title": "An isoform of hPANK2, deficient in pantothenate kinase-associated neurodegeneration, localizes to mitochondria", "journal": "Hum Mol Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12554685/" }, { "id": "PMID:15105273", "title": "Mitochondrial localization of human PANK2 and hypotheses of secondary iron accumulation in pantothenate kinase-associated neurodegeneration", "journal": "Ann N Y Acad Sci", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15105273/" }, { "id": "PMID:17242360", "title": "Activation of human mitochondrial pantothenate kinase 2 by palmitoylcarnitine", "journal": "Proc Natl Acad Sci U S A", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17242360/" }, { "id": "PMID:16040613", "title": "Feedback regulation of murine pantothenate kinase 3 by coenzyme A and coenzyme A thioesters", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16040613/" }, { "id": "PMID:16272150", "title": "Biochemical properties of human pantothenate kinase 2 isoforms and mutations linked to pantothenate kinase-associated neurodegeneration", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16272150/" }, { "id": "PMID:11479594", "title": "A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome", "journal": "Nat Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11479594/" } ], "x": 5948.666666666667, "y": 2910.4868421052633, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196929", "label": "FLAD1 phosphorylates FMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17238" ], "source_url": "https://reactome.org/content/detail/R-HSA-196929", "summary": "FLAD1 phosphorylates FMN is a Reactome release 97 curated reaction event. The source record connects FMN, ATP, H+ to FAD, PPi in cytosol and cross-references RHEA:17238.", "equation": "FMN + ATP + H+ → FAD + PPi", "inputs": [ { "node_id": "R-ALL-73524", "reactome_id": "R-ALL-73524", "label": "FMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58210", "formula": "C17H18N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29386", "reactome_id": "R-ALL-29386", "label": "FAD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "FMN adenylyltransferase activity of FLAD1 [cytosol]", "activity_id": "196924", "ec_numbers": [ "2.7.7.2" ], "accessions": [ "Q8NFF5" ] } ], "citations": [ { "id": "PMID:16643857", "title": "Over-expression in Escherichia coli and characterization of two recombinant isoforms of human FAD synthetase", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16643857/" } ], "x": 5391.333333333333, "y": 2920.7236842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196950", "label": "2xTRAP hydrolyzes FMN to RIB", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:35588" ], "source_url": "https://reactome.org/content/detail/R-HSA-196950", "summary": "2xTRAP hydrolyzes FMN to RIB is a Reactome release 97 curated reaction event. The source record connects FMN, H2O to RIB, Pi in cytosol and cross-references RHEA:35588.", "equation": "FMN + H2O → RIB + Pi", "inputs": [ { "node_id": "R-ALL-73524", "reactome_id": "R-ALL-73524", "label": "FMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58210", "formula": "C17H18N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196931", "reactome_id": "R-ALL-196931", "label": "RIB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57986", "formula": "C17H19N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acid phosphatase activity of 2xTRAP [cytosol]", "activity_id": "196922", "ec_numbers": [ "3.1.3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:2775236", "title": "Purification and characterization of a tartrate-resistant acid phosphatase from human osteoclastomas", "journal": "Biochem J", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2775236/" } ], "x": 5391.333333333333, "y": 2961.6710526315787, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196955", "label": "2xENPP1 hydrolyzes FAD to FMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [ "RHEA:13890" ], "source_url": "https://reactome.org/content/detail/R-HSA-196955", "summary": "2xENPP1 hydrolyzes FAD to FMN is a Reactome release 97 curated reaction event. The source record connects FAD, H2O to AMP, FMN, H+ in cytosol · plasma membrane and cross-references RHEA:13890.", "equation": "FAD + H2O → AMP + FMN + 2 H+", "inputs": [ { "node_id": "R-ALL-29386", "reactome_id": "R-ALL-29386", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73524", "reactome_id": "R-ALL-73524", "label": "FMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58210", "formula": "C17H18N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dinucleotide phosphatase activity of 2xENPP1 [plasma membrane]", "activity_id": "196942", "ec_numbers": [ "3.6.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:8001561", "title": "Biochemical characterization of human PC-1, an enzyme possessing alkaline phosphodiesterase I and nucleotide pyrophosphatase activities", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8001561/" } ], "x": 5670, "y": 2920.7236842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-196964", "label": "RFK:Mg2+ phosphorylates RIB", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14358" ], "source_url": "https://reactome.org/content/detail/R-HSA-196964", "summary": "RFK:Mg2+ phosphorylates RIB is a Reactome release 97 curated reaction event. The source record connects RIB, ATP to FMN, ADP, H+ in cytosol and cross-references RHEA:14358.", "equation": "RIB + ATP → FMN + ADP + H+", "inputs": [ { "node_id": "R-ALL-196931", "reactome_id": "R-ALL-196931", "label": "RIB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57986", "formula": "C17H19N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73524", "reactome_id": "R-ALL-73524", "label": "FMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58210", "formula": "C17H18N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "riboflavin kinase activity of RFK:Mg2+ [cytosol]", "activity_id": "196939", "ec_numbers": [ "2.7.1.26" ], "accessions": [] } ], "citations": [ { "id": "PMID:12623014", "title": "Crystal structure of human riboflavin kinase reveals a beta barrel fold and a novel active site arch", "journal": "Structure", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12623014/" } ], "x": 5948.666666666667, "y": 2920.7236842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1971482", "label": "CSGALNACTs transfer GalNAc to CS linker glycan", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:23465" ], "source_url": "https://reactome.org/content/detail/R-HSA-1971482", "summary": "CSGALNACTs transfer GalNAc to CS linker glycan is a Reactome release 97 curated reaction event. The source record connects UDP-GalNAc, GlcA-Gal-Gal-Xyl-CS proteins to UDP, chondroitin(1)-core proteins, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:23465.", "equation": "UDP-GalNAc + GlcA-Gal-Gal-Xyl-CS proteins → UDP + chondroitin(1)-core proteins + H+", "inputs": [ { "node_id": "R-ALL-735700", "reactome_id": "R-ALL-735700", "label": "UDP-GalNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57847", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064233", "reactome_id": "R-HSA-2064233", "label": "GlcA-Gal-Gal-Xyl-CS proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064172", "reactome_id": "R-HSA-2064172", "label": "chondroitin(1)-core proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity of CSGALNACT [Golgi membrane]", "activity_id": "1971478", "ec_numbers": [ "2.4.1.175" ], "accessions": [] } ], "citations": [ { "id": "PMID:11788602", "title": "Molecular cloning and expression of human chondroitin N-acetylgalactosaminyltransferase: the key enzyme for chain initiation and elongation of chondroitin/dermatan sulfate on the protein linkage region tetrasaccharide shared by heparin/heparan sulfate", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11788602/" }, { "id": "PMID:12163485", "title": "Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that transfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12163485/" }, { "id": "PMID:27599773", "title": "Chondroitin Sulfate N-acetylgalactosaminyltransferase-1 (CSGalNAcT-1) Deficiency Results in a Mild Skeletal Dysplasia and Joint Laxity", "journal": "Hum Mutat", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/27599773/" } ], "x": 2938.666666666667, "y": 806.8333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1971483", "label": "CHST11,12,13,9 transfer sulfate to (4-)GalNAc", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:16102" ], "source_url": "https://reactome.org/content/detail/R-HSA-1971483", "summary": "CHST11,12,13,9 transfer sulfate to (4-)GalNAc is a Reactome release 97 curated reaction event. The source record connects chondroitin(3)-core proteins, PAPS to C4S-PG, PAP, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:16102.", "equation": "chondroitin(3)-core proteins + PAPS → C4S-PG + PAP + H+", "inputs": [ { "node_id": "R-HSA-2064075", "reactome_id": "R-HSA-2064075", "label": "chondroitin(3)-core proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2064226", "reactome_id": "R-HSA-2064226", "label": "C4S-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "chondroitin 4-sulfotransferase activity of CHST9,11,12,13 [Golgi membrane]", "activity_id": "1971529", "ec_numbers": [ "2.8.2.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:11139592", "title": "Molecular cloning and expression of an N-acetylgalactosamine-4-O-sulfotransferase that transfers sulfate to terminal and non-terminal beta 1,4-linked N-acetylgalactosamine", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11139592/" }, { "id": "PMID:11056388", "title": "Molecular cloning, expression, and chromosomal mapping of human chondroitin 4-sulfotransferase, whose expression pattern in human tissues is different from that of chondroitin 6-sulfotransferase", "journal": "J Biochem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11056388/" }, { "id": "PMID:10781601", "title": "Molecular cloning and expression of two distinct human chondroitin 4-O-sulfotransferases that belong to the HNK-1 sulfotransferase gene family", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10781601/" }, { "id": "PMID:12080076", "title": "Molecular cloning and characterization of chondroitin-4-O-sulfotransferase-3. A novel member of the HNK-1 family of sulfotransferases", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12080076/" }, { "id": "PMID:29514872", "title": "Homozygous CHST11 mutation in chondrodysplasia, brachydactyly, overriding digits, clino-symphalangism and synpolydactyly", "journal": "J Med Genet", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29514872/" } ], "x": 2381.3333333333335, "y": 818.6212121212121, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1971487", "label": "Chondroitin polymerase complex transfers GalNAc to CS chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1971487", "summary": "Chondroitin polymerase complex transfers GalNAc to CS chain is a Reactome release 97 curated reaction event. The source record connects chondroitin(2)-core proteins, UDP-GalNAc to UDP, chondroitin(3)-core proteins, H+ in Golgi membrane · Golgi lumen.", "equation": "chondroitin(2)-core proteins + UDP-GalNAc → UDP + chondroitin(3)-core proteins + H+", "inputs": [ { "node_id": "R-HSA-2064050", "reactome_id": "R-HSA-2064050", "label": "chondroitin(2)-core proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-735700", "reactome_id": "R-ALL-735700", "label": "UDP-GalNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57847", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064075", "reactome_id": "R-HSA-2064075", "label": "chondroitin(3)-core proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity of Chondroitin polymerase complex [Golgi membrane]", "activity_id": "1971459", "ec_numbers": [ "2.4.1.175" ], "accessions": [] } ], "citations": [ { "id": "PMID:12761225", "title": "Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12761225/" }, { "id": "PMID:12907687", "title": "Chondroitin sulfate synthase-3. Molecular cloning and characterization", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12907687/" }, { "id": "PMID:11514575", "title": "Molecular cloning and expression of a human chondroitin synthase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11514575/" }, { "id": "PMID:21129727", "title": "Loss of CHSY1, a secreted FRINGE enzyme, causes syndromic brachydactyly in humans via increased NOTCH signaling", "journal": "Am J Hum Genet", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21129727/" }, { "id": "PMID:21129728", "title": "Temtamy preaxial brachydactyly syndrome is caused by loss-of-function mutations in chondroitin synthase 1, a potential target of BMP signaling", "journal": "Am J Hum Genet", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21129728/" }, { "id": "PMID:23774590", "title": "Biosynthesis and function of chondroitin sulfate", "journal": "Biochim Biophys Acta", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23774590/" } ], "x": 2381.3333333333335, "y": 995.439393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-1971491", "label": "Chondroitin polymerase complex transfers GlcA to CS chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-1971491", "summary": "Chondroitin polymerase complex transfers GlcA to CS chain is a Reactome release 97 curated reaction event. The source record connects UDP-GlcA, chondroitin(1)-core proteins to UDP, chondroitin(2)-core proteins, H+ in Golgi membrane · Golgi lumen.", "equation": "UDP-GlcA + chondroitin(1)-core proteins → UDP + chondroitin(2)-core proteins + H+", "inputs": [ { "node_id": "R-ALL-1889970", "reactome_id": "R-ALL-1889970", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064172", "reactome_id": "R-HSA-2064172", "label": "chondroitin(1)-core proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064050", "reactome_id": "R-HSA-2064050", "label": "chondroitin(2)-core proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity of Chondroitin polymerase complex [Golgi membrane]", "activity_id": "1971466", "ec_numbers": [ "2.4.1.226" ], "accessions": [] } ], "citations": [ { "id": "PMID:11514575", "title": "Molecular cloning and expression of a human chondroitin synthase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11514575/" }, { "id": "PMID:12761225", "title": "Chondroitin sulfate synthase-2. Molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase, which has dual enzymatic activities", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12761225/" }, { "id": "PMID:12907687", "title": "Chondroitin sulfate synthase-3. Molecular cloning and characterization", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12907687/" }, { "id": "PMID:18316376", "title": "Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: chondroitin polymerization is achieved by multiple enzyme complexes consisting of chondroitin synthase family members", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18316376/" }, { "id": "PMID:12145278", "title": "Molecular cloning and characterization of a novel chondroitin sulfate glucuronyltransferase that transfers glucuronic acid to N-acetylgalactosamine", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12145278/" }, { "id": "PMID:21129727", "title": "Loss of CHSY1, a secreted FRINGE enzyme, causes syndromic brachydactyly in humans via increased NOTCH signaling", "journal": "Am J Hum Genet", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21129727/" } ], "x": 2660, "y": 995.439393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197186", "label": "NAPRT1 dimer transfers PRIB to NCA to form NAMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:36164" ], "source_url": "https://reactome.org/content/detail/R-HSA-197186", "summary": "NAPRT1 dimer transfers PRIB to NCA to form NAMN is a Reactome release 97 curated reaction event. The source record connects NCA, PRPP, ATP to PPi, NAMN, ADP in cytosol and cross-references RHEA:36164.", "equation": "NCA + PRPP + ATP + H2O → PPi + NAMN + ADP + Pi", "inputs": [ { "node_id": "R-ALL-197230", "reactome_id": "R-ALL-197230", "label": "NCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32544", "formula": "C6H4NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8869359", "reactome_id": "R-ALL-8869359", "label": "NAMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57502", "formula": "C11H12NO9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinate phosphoribosyltransferase activity of NAPRT1 dimer [cytosol]", "activity_id": "197236", "ec_numbers": [ "6.3.4.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:4349867", "title": "Nicotinate phosphoribosyltransferase of human erythrocytes. Purification and properties.", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4349867/" }, { "id": "PMID:13563527", "title": "Biosynthesis of diphosphopyridine nucleotide. II. Enzymatic aspects.", "journal": "J Biol Chem", "year": 1958, "url": "https://pubmed.ncbi.nlm.nih.gov/13563527/" }, { "id": "PMID:17604275", "title": "Elevation of cellular NAD levels by nicotinic acid and involvement of nicotinic acid phosphoribosyltransferase in human cells", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17604275/" } ], "x": 5948.666666666667, "y": 2808.1184210526317, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197187", "label": "ACS transforms to QUIN non-enzymatically", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25148" ], "source_url": "https://reactome.org/content/detail/R-HSA-197187", "summary": "ACS transforms to QUIN non-enzymatically is a Reactome release 97 curated reaction event. The source record connects ACS to QUIN, H2O, H+ in cytosol and cross-references RHEA:25148.", "equation": "ACS → QUIN + H2O + H+", "inputs": [ { "node_id": "R-ALL-36027", "reactome_id": "R-ALL-36027", "label": "ACS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77612", "formula": "C7H6NO5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-197238", "reactome_id": "R-ALL-197238", "label": "QUIN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29959", "formula": "C7H3NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:15656614", "title": "The pyridine ring of NAD is formed by a nonenzymatic pericyclic reaction", "journal": "J Am Chem Soc", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15656614/" } ], "x": 5948.666666666667, "y": 2879.7763157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197198", "label": "NADK:Zn2+ tetramer phosphorylates NAD+ to NADP+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18630" ], "source_url": "https://reactome.org/content/detail/R-HSA-197198", "summary": "NADK:Zn2+ tetramer phosphorylates NAD+ to NADP+ is a Reactome release 97 curated reaction event. The source record connects NAD+, ATP to NADP+, ADP, H+ in cytosol and cross-references RHEA:18630.", "equation": "NAD+ + ATP → NADP+ + ADP + H+", "inputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NAD+ kinase activity of NADK:Zn2+ tetramer [cytosol]", "activity_id": "197237", "ec_numbers": [ "2.7.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:11594753", "title": "Structural and functional characterization of human NAD kinase", "journal": "Biochem Biophys Res Commun", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11594753/" } ], "x": 5670, "y": 2818.3552631578946, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197235", "label": "NMNAT2 transfers an adenylyl group from ATP to NAMN to yield NAAD", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · Golgi membrane", "compartment_ids": [ "GO:0005829", "GO:0000139" ], "rhea_ids": [ "RHEA:22861" ], "source_url": "https://reactome.org/content/detail/R-HSA-197235", "summary": "NMNAT2 transfers an adenylyl group from ATP to NAMN to yield NAAD is a Reactome release 97 curated reaction event. The source record connects ATP, NAMN, H+ to NAAD, PPi in cytosol · Golgi membrane and cross-references RHEA:22861.", "equation": "ATP + NAMN + H+ → NAAD + PPi", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8869359", "reactome_id": "R-ALL-8869359", "label": "NAMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57502", "formula": "C11H12NO9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-197256", "reactome_id": "R-ALL-197256", "label": "NAAD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58437", "formula": "C21H24N6O15P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinate-nucleotide adenylyltransferase activity of NMNAT2:Mg2+ [Golgi membrane]", "activity_id": "197217", "ec_numbers": [ "2.7.7.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:14516279", "title": "Characterization of human brain nicotinamide 5'-mononucleotide adenylyltransferase-2 and expression in human pancreas", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14516279/" }, { "id": "PMID:12359228", "title": "Identification of a novel human nicotinamide mononucleotide adenylyltransferase", "journal": "Biochem Biophys Res Commun", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359228/" }, { "id": "PMID:16118205", "title": "Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16118205/" }, { "id": "PMID:17402747", "title": "Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17402747/" } ], "x": 5948.666666666667, "y": 2818.3552631578946, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197250", "label": "NAMPT transfers PRIB to NAM to form NAMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16151" ], "source_url": "https://reactome.org/content/detail/R-HSA-197250", "summary": "NAMPT transfers PRIB to NAM to form NAMN is a Reactome release 97 curated reaction event. The source record connects NAM, PRPP, H+ to PPi, NAMN in cytosol and cross-references RHEA:16151.", "equation": "NAM + PRPP + H+ → PPi + NAMN", "inputs": [ { "node_id": "R-ALL-197277", "reactome_id": "R-ALL-197277", "label": "NAM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8869359", "reactome_id": "R-ALL-8869359", "label": "NAMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57502", "formula": "C11H12NO9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinamide phosphoribosyltransferase activity of NAMPT [cytosol]", "activity_id": "197240", "ec_numbers": [ "2.4.2.12" ], "accessions": [ "P43490" ] } ], "citations": [ { "id": "PMID:15381699", "title": "The NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase regulates Sir2 activity in mammalian cells", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15381699/" }, { "id": "PMID:8289818", "title": "Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor", "journal": "Mol Cell Biol", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8289818/" } ], "x": 5391.333333333333, "y": 2930.9605263157896, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197268", "label": "QPRT transfers PRIB to QUIN to form NAMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12735" ], "source_url": "https://reactome.org/content/detail/R-HSA-197268", "summary": "QPRT transfers PRIB to QUIN to form NAMN is a Reactome release 97 curated reaction event. The source record connects QUIN, PRPP, H+ to PPi, CO2, NAMN in cytosol and cross-references RHEA:12735.", "equation": "QUIN + PRPP + 2 H+ → PPi + CO2 + NAMN", "inputs": [ { "node_id": "R-ALL-197238", "reactome_id": "R-ALL-197238", "label": "QUIN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29959", "formula": "C7H3NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8869359", "reactome_id": "R-ALL-8869359", "label": "NAMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57502", "formula": "C11H12NO9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinate-nucleotide diphosphorylase (carboxylating) activity of QPRT [cytosol]", "activity_id": "197214", "ec_numbers": [ "2.4.2.19" ], "accessions": [ "Q15274" ] } ], "citations": [ { "id": "PMID:9473669", "title": "Characterization and functional expression of the cDNA encoding human brain quinolinate phosphoribosyltransferase", "journal": "Biochim Biophys Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9473669/" }, { "id": "PMID:17868694", "title": "Structural and kinetic characterization of quinolinate phosphoribosyltransferase (hQPRTase) from homo sapiens", "journal": "J Mol Biol", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17868694/" } ], "x": 5391.333333333333, "y": 2818.3552631578946, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197271", "label": "NADSYN1 hexamer amidates NAAD to NAD+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24385" ], "source_url": "https://reactome.org/content/detail/R-HSA-197271", "summary": "NADSYN1 hexamer amidates NAAD to NAD+ is a Reactome release 97 curated reaction event. The source record connects NAAD, ATP, H2O to NAD+, AMP, PPi in cytosol and cross-references RHEA:24385.", "equation": "NAAD + ATP + H2O + L-Gln → NAD+ + AMP + PPi + L-Glu + H+", "inputs": [ { "node_id": "R-ALL-197256", "reactome_id": "R-ALL-197256", "label": "NAAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58437", "formula": "C21H24N6O15P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NAD+ synthase (glutamine-hydrolyzing) activity of NADSYN1 hexamer [cytosol]", "activity_id": "197281", "ec_numbers": [ "6.3.5.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12547821", "title": "Molecular identification of human glutamine- and ammonia-dependent NAD synthetases. Carbon-nitrogen hydrolase domain confers glutamine dependency.", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12547821/" } ], "x": 5670, "y": 2808.1184210526317, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197958", "label": "FPGS-2 transforms THF to THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-197958", "summary": "FPGS-2 transforms THF to THFPG is a Reactome release 97 curated reaction event. The source record connects THF, ATP, L-Glu to THFPG, ADP, Pi in cytosol.", "equation": "THF + 5 ATP + 5 L-Glu → THFPG + 5 ADP + 5 Pi", "inputs": [ { "node_id": "R-ALL-111355", "reactome_id": "R-ALL-111355", "label": "THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 5, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 5, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-197969", "reactome_id": "R-ALL-197969", "label": "THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28624", "formula": "(C5H7NO3)n.C19H23N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 5, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 5, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "tetrahydrofolylpolyglutamate synthase activity of FPGS-2 [cytosol]", "activity_id": "197957", "ec_numbers": [ "6.3.2.17" ], "accessions": [ "Q05932" ] } ], "citations": [ { "id": "PMID:1409616", "title": "Expression cloning of a human cDNA encoding folylpoly(gamma-glutamate) synthetase and determination of its primary structure", "journal": "Proc Natl Acad Sci U S A", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1409616/" }, { "id": "PMID:8662720", "title": "Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662720/" } ], "x": 4730, "y": 2926.7727272727275, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197963", "label": "DHFR2 reduces FOLA to DHF", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:31105" ], "source_url": "https://reactome.org/content/detail/R-HSA-197963", "summary": "DHFR2 reduces FOLA to DHF is a Reactome release 97 curated reaction event. The source record connects FOLA, NADPH, H+ to DHF, NADP+ in cytosol and cross-references RHEA:31105.", "equation": "FOLA + NADPH + H+ → DHF + NADP+", "inputs": [ { "node_id": "R-ALL-197965", "reactome_id": "R-ALL-197965", "label": "FOLA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62501", "formula": "C19H17N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73604", "reactome_id": "R-ALL-73604", "label": "DHF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57451", "formula": "C19H19N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "folate reductase activity of DHFR2 [cytosol]", "activity_id": "197966", "ec_numbers": null, "accessions": [ "Q86XF0" ] } ], "citations": [ { "id": "PMID:6323448", "title": "The functional human dihydrofolate reductase gene", "journal": "J Biol Chem", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6323448/" } ], "x": 4451.333333333333, "y": 2820.681818181818, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-197972", "label": "DHF is reduced to tetrahydrofolate (THF)", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15011" ], "source_url": "https://reactome.org/content/detail/R-HSA-197972", "summary": "DHF is reduced to tetrahydrofolate (THF) is a Reactome release 97 curated reaction event. The source record connects DHF, NADPH, H+ to THF, NADP+ in cytosol and cross-references RHEA:15011.", "equation": "DHF + NADPH + H+ → THF + NADP+", "inputs": [ { "node_id": "R-ALL-73604", "reactome_id": "R-ALL-73604", "label": "DHF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57451", "formula": "C19H19N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111355", "reactome_id": "R-ALL-111355", "label": "THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "folate reductase activity of DHFR2 [cytosol]", "activity_id": "197966", "ec_numbers": null, "accessions": [ "Q86XF0" ] } ], "citations": [ { "id": "PMID:6323448", "title": "The functional human dihydrofolate reductase gene", "journal": "J Biol Chem", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6323448/" }, { "id": "PMID:2248959", "title": "Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate", "journal": "Biochemistry", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2248959/" } ], "x": 4730, "y": 2820.681818181818, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-198458", "label": "Efflux of Glc from the endoplasmic reticulum", "schema_class": "BlackBoxEvent", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane · cytosol · plasma membrane · extracellular region", "compartment_ids": [ "GO:0005788", "GO:0005789", "GO:0005829", "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:60376" ], "source_url": "https://reactome.org/content/detail/R-HSA-198458", "summary": "Efflux of Glc from the endoplasmic reticulum is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Glc to Glc in endoplasmic reticulum lumen · endoplasmic reticulum membrane · cytosol · plasma membrane · extracellular region and cross-references RHEA:60376.", "equation": "Glc → Glc", "inputs": [ { "node_id": "R-ALL-113780", "reactome_id": "R-ALL-113780", "label": "Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70115", "reactome_id": "R-ALL-70115", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16314530", "title": "Evidence for high-capacity bidirectional glucose transport across the endoplasmic reticulum membrane by genetically encoded fluoresence resonance energy transfer nanosensors", "journal": "Mol Cell Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16314530/" }, { "id": "PMID:11882499", "title": "Glucose release from GLUT2-null hepatocytes: characterization of a major and a minor pathway", "journal": "Am J Physiol Endocrinol Metab", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11882499/" }, { "id": "PMID:11879177", "title": "The glucose-6-phosphatase system", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11879177/" } ], "x": 1530, "y": 619.5833333333333, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-198513", "label": "SLC7A4 exchanges G6P for Pi across the ER membrane", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol · endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005829", "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:71537" ], "source_url": "https://reactome.org/content/detail/R-HSA-198513", "summary": "SLC7A4 exchanges G6P for Pi across the ER membrane is a Reactome release 97 curated reaction event. The source record connects G6P, Pi to G6P, Pi in cytosol · endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:71537.", "equation": "G6P + Pi → G6P + Pi", "inputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113551", "reactome_id": "R-ALL-113551", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113782", "reactome_id": "R-ALL-113782", "label": "G6P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucose 6-phosphate:phosphate antiporter activity of SLC37A4 [endoplasmic reticulum membrane]", "activity_id": "198473", "ec_numbers": null, "accessions": [ "O43826" ] } ], "citations": [ { "id": "PMID:18337460", "title": "The glucose-6-phosphate transporter is a phosphate-linked antiporter deficient in glycogen storage disease type Ib and Ic", "journal": "FASEB J.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18337460/" }, { "id": "PMID:9428641", "title": "Sequence of a putative glucose 6-phosphate translocase, mutated in glycogen storage disease type Ib", "journal": "FEBS Lett", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9428641/" }, { "id": "PMID:9758626", "title": "A gene on chromosome 11q23 coding for a putative glucose- 6-phosphate translocase is mutated in glycogen-storage disease types Ib and Ic", "journal": "Am. J. Hum. Genet.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9758626/" } ], "x": 1808.6666666666667, "y": 489.91666666666663, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-198563", "label": "IDO1 dioxygenates L-Trp to NFK", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24537" ], "source_url": "https://reactome.org/content/detail/R-HSA-198563", "summary": "IDO1 dioxygenates L-Trp to NFK is a Reactome release 97 curated reaction event. The source record connects L-Trp, O2 to NFK in cytosol and cross-references RHEA:24537.", "equation": "L-Trp + O2 → NFK", "inputs": [ { "node_id": "R-ALL-29500", "reactome_id": "R-ALL-29500", "label": "L-Trp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57912", "formula": "C11H12N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-33179", "reactome_id": "R-ALL-33179", "label": "NFK", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58629", "formula": "C11H12N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-tryptophan 2,3-dioxygenase activity of IDO1 [cytosol]", "activity_id": "198570", "ec_numbers": [ "1.13.11.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:16477023", "title": "Crystal structure of human indoleamine 2,3-dioxygenase: catalytic mechanism of O2 incorporation by a heme-containing dioxygenase", "journal": "Proc Natl Acad Sci USA", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16477023/" } ], "x": 8018.666666666667, "y": 1834.7916666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-198813", "label": "GSTO dimers reduce DeHA to AscH-", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24425" ], "source_url": "https://reactome.org/content/detail/R-HSA-198813", "summary": "GSTO dimers reduce DeHA to AscH- is a Reactome release 97 curated reaction event. The source record connects DeHA, GSH to AscH-, GSSG in cytosol and cross-references RHEA:24425.", "equation": "DeHA + 2 GSH → AscH- + GSSG", "inputs": [ { "node_id": "R-ALL-198827", "reactome_id": "R-ALL-198827", "label": "DeHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58539", "formula": "C6H5O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-198816", "reactome_id": "R-ALL-198816", "label": "AscH-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:38290", "formula": "C6H7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione dehydrogenase (ascorbate) activity of GSTO dimers [cytosol]", "activity_id": "198791", "ec_numbers": [ "1.8.5.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:15970797", "title": "Characterization of the monomethylarsonate reductase and dehydroascorbate reductase activities of Omega class glutathione transferase variants: implications for arsenic metabolism and the age-at-onset of Alzheimer's and Parkinson's diseases", "journal": "Pharmacogenet Genomics", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15970797/" }, { "id": "PMID:10783391", "title": "Identification, characterization, and crystal structure of the Omega class glutathione transferases", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10783391/" } ], "x": 5670, "y": 2961.6710526315787, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-198818", "label": "SLC2A1,3 transports DeHA from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:60380" ], "source_url": "https://reactome.org/content/detail/R-HSA-198818", "summary": "SLC2A1,3 transports DeHA from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects DeHA to DeHA in plasma membrane and cross-references RHEA:60380.", "equation": "DeHA → DeHA", "inputs": [ { "node_id": "R-ALL-198852", "reactome_id": "R-ALL-198852", "label": "DeHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58539", "formula": "C6H5O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-198827", "reactome_id": "R-ALL-198827", "label": "DeHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58539", "formula": "C6H5O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dehydroascorbic acid transmembrane transporter activity of SLC2A1,3 [plasma membrane]", "activity_id": "198847", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9228080", "title": "Glucose transporter isoforms GLUT1 and GLUT3 transport dehydroascorbic acid", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9228080/" }, { "id": "PMID:10862609", "title": "Dehydroascorbic acid transport by GLUT4 in Xenopus oocytes and isolated rat adipocytes", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10862609/" } ], "x": 5948.666666666667, "y": 3012.8552631578946, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-198824", "label": "CYB5R3:FAD reduces CYB5A:ferriheme to CYB5A:heme", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-198824", "summary": "CYB5R3:FAD reduces CYB5A:ferriheme to CYB5A:heme is a Reactome release 97 curated reaction event. The source record connects CYB5A:ferriheme, NADH, H+ to CYB5A:heme, NAD+ in cytosol · mitochondrial outer membrane.", "equation": "CYB5A:ferriheme + NADH + H+ → CYB5A:heme + NAD+", "inputs": [ { "node_id": "R-HSA-198772", "reactome_id": "R-HSA-198772", "label": "CYB5A:ferriheme", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-198808", "reactome_id": "R-HSA-198808", "label": "CYB5A:heme", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cytochrome-b5 reductase activity, acting on NAD(P)H of CYB5R3:FAD [mitochondrial outer membrane]", "activity_id": "198863", "ec_numbers": [ "1.6.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8513896", "title": "NADH-cytochrome b5 reductase and cytochrome b5 isoforms as models for the study of post-translational targeting to the endoplasmic reticulum", "journal": "FEBS Lett", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8513896/" }, { "id": "PMID:7668255", "title": "A novel point mutation in a 3' splice site of the NADH-cytochrome b5 reductase gene results in immunologically undetectable enzyme and impaired NADH-dependent ascorbate regeneration in cultured fibroblasts of a patient with type II hereditary methemoglobinemia", "journal": "Am J Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7668255/" } ], "x": 5670, "y": 3084.5131578947367, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-198845", "label": "CYB5A:heme reduces Asc.- to AscH-", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-198845", "summary": "CYB5A:heme reduces Asc.- to AscH- is a Reactome release 97 curated reaction event. The source record connects Asc.-, CYB5A:heme to AscH-, CYB5A:ferriheme in cytosol · mitochondrial outer membrane.", "equation": "Asc.- + CYB5A:heme → AscH- + CYB5A:ferriheme", "inputs": [ { "node_id": "R-ALL-1497805", "reactome_id": "R-ALL-1497805", "label": "Asc.-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59513", "formula": "C6H6O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-198808", "reactome_id": "R-HSA-198808", "label": "CYB5A:heme", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-198816", "reactome_id": "R-ALL-198816", "label": "AscH-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:38290", "formula": "C6H7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-198772", "reactome_id": "R-HSA-198772", "label": "CYB5A:ferriheme", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of CYB5A:heme [mitochondrial outer membrane]", "activity_id": "198774", "ec_numbers": [ "1.1.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:7668255", "title": "A novel point mutation in a 3' splice site of the NADH-cytochrome b5 reductase gene results in immunologically undetectable enzyme and impaired NADH-dependent ascorbate regeneration in cultured fibroblasts of a patient with type II hereditary methemoglobinemia", "journal": "Am J Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7668255/" } ], "x": 5948.666666666667, "y": 3094.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-198870", "label": "SLC23A1,2 cotransports AscH-, 2Na+ from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:69883" ], "source_url": "https://reactome.org/content/detail/R-HSA-198870", "summary": "SLC23A1,2 cotransports AscH-, 2Na+ from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects AscH-, Na+ to AscH-, Na+ in plasma membrane and cross-references RHEA:69883.", "equation": "AscH- + 2 Na+ → AscH- + 2 Na+", "inputs": [ { "node_id": "R-ALL-198836", "reactome_id": "R-ALL-198836", "label": "AscH-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:38290", "formula": "C6H7O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-74113", "reactome_id": "R-ALL-74113", "label": "Na+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-198816", "reactome_id": "R-ALL-198816", "label": "AscH-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:38290", "formula": "C6H7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-83910", "reactome_id": "R-ALL-83910", "label": "Na+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-ascorbic acid transmembrane transporter activity of SLC23A1,2 [plasma membrane]", "activity_id": "198796", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11396616", "title": "Vitamin C transport systems of mammalian cells", "journal": "Mol Membr Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11396616/" }, { "id": "PMID:10471399", "title": "Human placental sodium-dependent vitamin C transporter (SVCT2): molecular cloning and transport function", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10471399/" }, { "id": "PMID:10556521", "title": "Cloning and functional characterization of the human sodium-dependent vitamin C transporters hSVCT1 and hSVCT2", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10556521/" }, { "id": "PMID:10556483", "title": "Human Na+-dependent vitamin C transporter 1 (hSVCT1): primary structure, functional characteristics and evidence for a non-functional splice variant", "journal": "Biochim Biophys Acta", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10556483/" } ], "x": 5948.666666666667, "y": 2961.6710526315787, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-199202", "label": "Phosphopantetheine conjugation of the ACP domain of FAS", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-199202", "summary": "Phosphopantetheine conjugation of the ACP domain of FAS is a Reactome release 97 curated reaction event. The source record connects CoA-SH, FASN to FASN, PAP in cytosol.", "equation": "CoA-SH + FASN → FASN + PAP", "inputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-199233", "reactome_id": "R-HSA-199233", "label": "FASN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-54659", "reactome_id": "R-HSA-54659", "label": "FASN", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "holo-[acyl-carrier-protein] synthase activity of AASDHPPT [cytosol]", "activity_id": "199214", "ec_numbers": [ "2.7.8.7" ], "accessions": [ "Q9NRN7" ] } ], "citations": [ { "id": "PMID:12815048", "title": "Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12815048/" } ], "x": 5391.333333333333, "y": 3104.9868421052633, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-199203", "label": "PANK1/3 phosphorylate PanK", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16374" ], "source_url": "https://reactome.org/content/detail/R-HSA-199203", "summary": "PANK1/3 phosphorylate PanK is a Reactome release 97 curated reaction event. The source record connects PanK, ATP to PPanK, ADP, H+ in cytosol and cross-references RHEA:16374.", "equation": "PanK + ATP → PPanK + ADP + H+", "inputs": [ { "node_id": "R-ALL-196820", "reactome_id": "R-ALL-196820", "label": "PanK", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29032", "formula": "C9H16NO5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-196786", "reactome_id": "R-ALL-196786", "label": "PPanK", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:10986", "formula": "C9H15NO8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pantothenate kinase activity of PANK1/3 [cytosol]", "activity_id": "199185", "ec_numbers": [ "2.7.1.33" ], "accessions": [] } ], "citations": [ { "id": "PMID:36983025", "title": "Inherited Disorders of Coenzyme A Biosynthesis: Models, Mechanisms, and Treatments", "journal": "Int J Mol Sci", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36983025/" }, { "id": "PMID:15893380", "title": "Coenzyme A: back in action", "journal": "Prog Lipid Res", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15893380/" }, { "id": "PMID:14523052", "title": "PPARalpha controls the intracellular coenzyme A concentration via regulation of PANK1alpha gene expression", "journal": "J Lipid Res", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14523052/" }, { "id": "PMID:12095677", "title": "The murine pantothenate kinase (Pank1) gene encodes two differentially regulated pantothenate kinase isozymes", "journal": "Gene", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12095677/" }, { "id": "PMID:11479594", "title": "A novel pantothenate kinase gene (PANK2) is defective in Hallervorden-Spatz syndrome", "journal": "Nat Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11479594/" } ], "x": 5670, "y": 2930.9605263157896, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-199206", "label": "SLC5A6 cotransports extracellular PanK and 2Na+ to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:73371" ], "source_url": "https://reactome.org/content/detail/R-HSA-199206", "summary": "SLC5A6 cotransports extracellular PanK and 2Na+ to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PanK, Na+ to PanK, Na+ in plasma membrane and cross-references RHEA:73371.", "equation": "PanK + 2 Na+ → PanK + 2 Na+", "inputs": [ { "node_id": "R-ALL-199192", "reactome_id": "R-ALL-199192", "label": "PanK", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29032", "formula": "C9H16NO5", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-74113", "reactome_id": "R-ALL-74113", "label": "Na+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-196820", "reactome_id": "R-ALL-196820", "label": "PanK", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29032", "formula": "C9H16NO5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-83910", "reactome_id": "R-ALL-83910", "label": "Na+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sodium-dependent multivitamin transmembrane transporter activity of SLC5A6:PDZD11 [plasma membrane]", "activity_id": "199212", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:21183659", "title": "Association of PDZ-containing protein PDZD11 with the human sodium-dependent multivitamin transporter", "journal": "Am. J. Physiol. Gastrointest. Liver Physiol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21183659/" }, { "id": "PMID:9516450", "title": "Cloning and functional expression of a cDNA encoding a mammalian sodium-dependent vitamin transporter mediating the uptake of pantothenate, biotin, and lipoate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9516450/" }, { "id": "PMID:10334869", "title": "Molecular and functional characterization of the intestinal Na+-dependent multivitamin transporter", "journal": "Arch Biochem Biophys", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10334869/" }, { "id": "PMID:10329687", "title": "Human placental Na+-dependent multivitamin transporter. Cloning, functional expression, gene structure, and chromosomal localization.", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10329687/" } ], "x": 5391.333333333333, "y": 2971.907894736842, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-199216", "label": "SLC25A16 transports cytosolic CoA-SH to mitochondrial matrix", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-199216", "summary": "SLC25A16 transports cytosolic CoA-SH to mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CoA-SH to CoA-SH in mitochondrial inner membrane.", "equation": "CoA-SH → CoA-SH", "inputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "coenzyme A transmembrane transporter activity of SLC25A16 [mitochondrial inner membrane]", "activity_id": "199228", "ec_numbers": null, "accessions": [ "P16260" ] } ], "citations": [ { "id": "PMID:17242360", "title": "Activation of human mitochondrial pantothenate kinase 2 by palmitoylcarnitine", "journal": "Proc Natl Acad Sci U S A", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17242360/" }, { "id": "PMID:11158296", "title": "The yeast mitochondrial carrier Leu5p and its human homologue Graves' disease protein are required for accumulation of coenzyme A in the matrix", "journal": "Mol Cell Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11158296/" }, { "id": "PMID:32234503", "title": "Regulation of coenzyme A levels by degradation: the 'Ins and Outs'", "journal": "Prog Lipid Res", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32234503/" } ], "x": 5670, "y": 3104.9868421052633, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-199219", "label": "SLC5A6 cotransports extracellular Btn and 2xNa+ to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:73375" ], "source_url": "https://reactome.org/content/detail/R-HSA-199219", "summary": "SLC5A6 cotransports extracellular Btn and 2xNa+ to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects Btn, Na+ to Btn, Na+ in plasma membrane and cross-references RHEA:73375.", "equation": "Btn + 2 Na+ → Btn + 2 Na+", "inputs": [ { "node_id": "R-ALL-199238", "reactome_id": "R-ALL-199238", "label": "Btn", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-74113", "reactome_id": "R-ALL-74113", "label": "Na+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-199207", "reactome_id": "R-ALL-199207", "label": "Btn", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-83910", "reactome_id": "R-ALL-83910", "label": "Na+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sodium-dependent multivitamin transmembrane transporter activity of SLC5A6:PDZD11 [plasma membrane]", "activity_id": "199212", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10329687", "title": "Human placental Na+-dependent multivitamin transporter. Cloning, functional expression, gene structure, and chromosomal localization.", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10329687/" }, { "id": "PMID:10334869", "title": "Molecular and functional characterization of the intestinal Na+-dependent multivitamin transporter", "journal": "Arch Biochem Biophys", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10334869/" }, { "id": "PMID:12646417", "title": "Biotin uptake by human intestinal and liver epithelial cells: role of the SMVT system", "journal": "Am. J. Physiol. Gastrointest. Liver Physiol.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12646417/" }, { "id": "PMID:21183659", "title": "Association of PDZ-containing protein PDZD11 with the human sodium-dependent multivitamin transporter", "journal": "Am. J. Physiol. Gastrointest. Liver Physiol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21183659/" } ], "x": 5670, "y": 2971.907894736842, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-199456", "label": "PTEN dephosphorylates PIP3", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [ "RHEA:25017" ], "source_url": "https://reactome.org/content/detail/R-HSA-199456", "summary": "PTEN dephosphorylates PIP3 is a Reactome release 97 curated reaction event. The source record connects PI(3,4,5)P3, H2O to PI(4,5)P2, Pi in cytosol · plasma membrane and cross-references RHEA:25017.", "equation": "PI(3,4,5)P3 + H2O → PI(4,5)P2 + Pi", "inputs": [ { "node_id": "R-ALL-179838", "reactome_id": "R-ALL-179838", "label": "PI(3,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57836", "formula": "C11H13O22P4R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-179856", "reactome_id": "R-ALL-179856", "label": "PI(4,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity of PTEN [cytosol]", "activity_id": "199445", "ec_numbers": [ "3.1.3.67" ], "accessions": [ "P60484" ] } ], "citations": [ { "id": "PMID:12808147", "title": "Membrane-binding and activation mechanism of PTEN", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12808147/" }, { "id": "PMID:9593664", "title": "The tumor suppressor, PTEN/MMAC1, dephosphorylates the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9593664/" }, { "id": "PMID:9811831", "title": "The lipid phosphatase activity of PTEN is critical for its tumor supressor function", "journal": "Proc Natl Acad Sci U S A", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9811831/" }, { "id": "PMID:10555148", "title": "Crystal structure of the PTEN tumor suppressor: implications for its phosphoinositide phosphatase activity and membrane association", "journal": "Cell", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10555148/" }, { "id": "PMID:8681945", "title": "Purification and characterization of the phosphatidylinositol-3,4,5-trisphosphate phosphatase in bovine thymus", "journal": "Eur. J. Biochem.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8681945/" } ], "x": 2938.666666666667, "y": 1631.5821917808219, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-199626", "label": "SLC19A2/3 transport extracellular THMN to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:34919" ], "source_url": "https://reactome.org/content/detail/R-HSA-199626", "summary": "SLC19A2/3 transport extracellular THMN to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects THMN to THMN in plasma membrane and cross-references RHEA:34919.", "equation": "THMN → THMN", "inputs": [ { "node_id": "R-ALL-199622", "reactome_id": "R-ALL-199622", "label": "THMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18385", "formula": "C12H17N4OS", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-196934", "reactome_id": "R-ALL-196934", "label": "THMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18385", "formula": "C12H17N4OS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "thiamine transmembrane transporter activity of SLC19A2/3 [plasma membrane]", "activity_id": "199654", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:16705148", "title": "Thiamin uptake by the human-derived renal epithelial (HEK-293) cells: cellular and molecular mechanisms", "journal": "Am J Physiol Renal Physiol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16705148/" }, { "id": "PMID:10391223", "title": "Mutations in a new gene encoding a thiamine transporter cause thiamine-responsive megaloblastic anaemia syndrome", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10391223/" }, { "id": "PMID:10391222", "title": "The gene mutated in thiamine-responsive anaemia with diabetes and deafness (TRMA) encodes a functional thiamine transporter", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10391222/" }, { "id": "PMID:10391221", "title": "Mutations in SLC19A2 cause thiamine-responsive megaloblastic anaemia associated with diabetes mellitus and deafness", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10391221/" }, { "id": "PMID:14615284", "title": "Expression and functional contribution of hTHTR-2 in thiamin absorption in human intestine", "journal": "Am J Physiol Gastrointest Liver Physiol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14615284/" }, { "id": "PMID:16371350", "title": "Targeting and trafficking of the human thiamine transporter-2 in epithelial cells", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16371350/" } ], "x": 5391.333333333333, "y": 3023.092105263158, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-199803", "label": "RPE dimers isomerize XY5P to RU5P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13679" ], "source_url": "https://reactome.org/content/detail/R-HSA-199803", "summary": "RPE dimers isomerize XY5P to RU5P is a Reactome release 97 curated reaction event. The source record connects XY5P to RU5P in cytosol and cross-references RHEA:13679.", "equation": "XY5P → RU5P", "inputs": [ { "node_id": "R-ALL-29790", "reactome_id": "R-ALL-29790", "label": "XY5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57737", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29732", "reactome_id": "R-ALL-29732", "label": "RU5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58121", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "D-ribulose-phosphate 3-epimerase activity of RPE:Fe2+ dimers [cytosol]", "activity_id": "71302", "ec_numbers": [ "5.1.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2581946", "title": "Phosphoribosylpyrophosphate synthesis from glucose decreases during amino acid starvation of human lymphoblasts", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2581946/" }, { "id": "PMID:7396409", "title": "Biochemical genetics of the pentose phosphate cycle: human ribose 5-phosphate isomerase (RPI) and ribulose 5-phosphate 3-epimerase (RPE)", "journal": "Ann Hum Genet", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/7396409/" } ], "x": 590, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200406", "label": "CPT1A transfers PALM to CAR", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "cytosol · mitochondrial outer membrane · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005829", "GO:0005741", "GO:0005758" ], "rhea_ids": [ "RHEA:12661", "RHEA:12662" ], "source_url": "https://reactome.org/content/detail/R-HSA-200406", "summary": "CPT1A transfers PALM to CAR is a Reactome release 97 curated reaction event. The source record connects CAR, PALM-CoA to CoA-SH, L-PCAR in cytosol · mitochondrial outer membrane · mitochondrial intermembrane space and cross-references RHEA:12661, RHEA:12662.", "equation": "CAR + PALM-CoA → CoA-SH + L-PCAR", "inputs": [ { "node_id": "R-ALL-549207", "reactome_id": "R-ALL-549207", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-200993", "reactome_id": "R-ALL-200993", "label": "PALM-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57379", "formula": "C37H62N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-201034", "reactome_id": "R-ALL-201034", "label": "L-PCAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17490", "formula": "C23H45NO4", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carnitine O-palmitoyltransferase activity of CPT1A [mitochondrial outer membrane]", "activity_id": "201041", "ec_numbers": [ "2.3.1.21" ], "accessions": [ "P50416" ] } ], "citations": [ { "id": "PMID:14517221", "title": "Functional and structural basis of carnitine palmitoyltransferase 1A deficiency", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14517221/" }, { "id": "PMID:9691089", "title": "Molecular basis of hepatic carnitine palmitoyltransferase I deficiency", "journal": "J Clin Invest", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9691089/" }, { "id": "PMID:11790793", "title": "Structural model of a malonyl-CoA-binding site of carnitine octanoyltransferase and carnitine palmitoyltransferase I: mutational analysis of a malonyl-CoA affinity domain", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11790793/" }, { "id": "PMID:14711372", "title": "Structural model of carnitine palmitoyltransferase I based on the carnitine acetyltransferase crystal", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14711372/" }, { "id": "PMID:11356169", "title": "Structure-function relationships of the liver and muscle isoforms of carnitine palmitoyltransferase I", "journal": "Biochem Soc Trans", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11356169/" }, { "id": "PMID:9344464", "title": "Functional studies of yeast-expressed human heart muscle carnitine palmitoyltransferase I", "journal": "Arch Biochem Biophys", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9344464/" } ], "x": 868.6666666666667, "y": 1573.7368421052631, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200410", "label": "CPT2 converts acylcarnitine to acyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:12663", "RHEA:40281" ], "source_url": "https://reactome.org/content/detail/R-HSA-200410", "summary": "CPT2 converts acylcarnitine to acyl-CoA is a Reactome release 97 curated reaction event. The source record connects CoA-SH, C8-C18 Acyl-CAR to CAR, C8-C18 Acyl-CoA in mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:12663, RHEA:40281.", "equation": "CoA-SH + C8-C18 Acyl-CAR → CAR + C8-C18 Acyl-CoA", "inputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971105", "reactome_id": "R-ALL-9971105", "label": "C8-C18 Acyl-CAR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-201023", "reactome_id": "R-ALL-201023", "label": "CAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9971103", "reactome_id": "R-ALL-9971103", "label": "C8-C18 Acyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "carnitine O-acyltransferase activity of CPT2 [mitochondrial inner membrane]", "activity_id": "201039", "ec_numbers": [ "2.3.1" ], "accessions": [ "P23786" ] } ], "citations": [ { "id": "PMID:7711730", "title": "Carnitine palmitoyltransferase II deficiency: structure of the gene and characterization of two novel disease-causing mutations", "journal": "Hum Mol Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7711730/" }, { "id": "PMID:20538056", "title": "Carnitine palmitoyltransferase 2: New insights on the substrate specificity and implications for acylcarnitine profiling", "journal": "Biochim Biophys Acta", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20538056/" } ], "x": 311.33333333333337, "y": 1967, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200423", "label": "pAMPK inactivates ACACB, inhibiting malonyl-CoA synthesis", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:17990" ], "source_url": "https://reactome.org/content/detail/R-HSA-200423", "summary": "pAMPK inactivates ACACB, inhibiting malonyl-CoA synthesis is a Reactome release 97 curated reaction event. The source record connects Btn-ACACB:2Mn2+ polymer, ATP to Btn-p-S222-ACACB:2Mn2+ polymer, ADP, H+ in cytosol · mitochondrial outer membrane and cross-references RHEA:17990.", "equation": "Btn-ACACB:2Mn2+ polymer + ATP → Btn-p-S222-ACACB:2Mn2+ polymer + ADP + H+", "inputs": [ { "node_id": "R-HSA-8867341", "reactome_id": "R-HSA-8867341", "label": "Btn-ACACB:2Mn2+ polymer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Polymer", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-8876893", "reactome_id": "R-HSA-8876893", "label": "Btn-p-S222-ACACB:2Mn2+ polymer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Polymer", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "AMP-activated protein kinase activity of AMPK heterotrimer (active) [cytosol]", "activity_id": "200412", "ec_numbers": [ "2.7.11.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:15060529", "title": "AMP kinase and malonyl-CoA: targets for therapy of the metabolic syndrome", "journal": "Nat Rev Drug Discov", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15060529/" } ], "x": 311.33333333333337, "y": 1685.3859649122808, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200424", "label": "Exchange of acylcarnitine and carnitine across the inner mitochondrial membrane", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "mitochondrial intermembrane space · mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005758", "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:49916", "RHEA:49926" ], "source_url": "https://reactome.org/content/detail/R-HSA-200424", "summary": "Exchange of acylcarnitine and carnitine across the inner mitochondrial membrane is a Reactome release 97 curated reaction event. The source record connects CAR, Acyl-CAR to CAR, Acyl-CAR in mitochondrial intermembrane space · mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:49916, RHEA:49926.", "equation": "CAR + Acyl-CAR → CAR + Acyl-CAR", "inputs": [ { "node_id": "R-ALL-201023", "reactome_id": "R-ALL-201023", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9971099", "reactome_id": "R-ALL-9971099", "label": "Acyl-CAR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-549207", "reactome_id": "R-ALL-549207", "label": "CAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9971097", "reactome_id": "R-ALL-9971097", "label": "Acyl-CAR", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "carnitine:O-acyl-L-carnitine antiporter activity of SLC25A20 [mitochondrial inner membrane]", "activity_id": "200567", "ec_numbers": null, "accessions": [ "O43772" ] } ], "citations": [ { "id": "PMID:9399886", "title": "Cloning of the human carnitine-acylcarnitine carrier cDNA and identification of the molecular defect in a patient", "journal": "Am J Hum Genet", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9399886/" } ], "x": 590, "y": 1625.0350877192982, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200474", "label": "NMNAT3 transfers an adenylyl group from ATP to NAMN to yield NAAD", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:22861", "RHEA:21361" ], "source_url": "https://reactome.org/content/detail/R-HSA-200474", "summary": "NMNAT3 transfers an adenylyl group from ATP to NAMN to yield NAAD is a Reactome release 97 curated reaction event. The source record connects NAMN, ATP, H+ to NAAD, PPi in mitochondrial matrix and cross-references RHEA:22861, RHEA:21361.", "equation": "NAMN + ATP + H+ → NAAD + PPi", "inputs": [ { "node_id": "R-ALL-8938083", "reactome_id": "R-ALL-8938083", "label": "NAMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57502", "formula": "C11H12NO9P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8938090", "reactome_id": "R-ALL-8938090", "label": "NAAD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58437", "formula": "C21H24N6O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinate-nucleotide adenylyltransferase activity of 4xNMNAT3:4xMg2+ [mitochondrial matrix]", "activity_id": "200494", "ec_numbers": [ "2.7.7.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:16118205", "title": "Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16118205/" }, { "id": "PMID:17402747", "title": "Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17402747/" }, { "id": "PMID:12574164", "title": "Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12574164/" } ], "x": 5391.333333333333, "y": 2828.592105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200512", "label": "NMNAT1 transfers an adenylyl group from ATP to NAMN to yield NAAD", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:22861", "RHEA:21361" ], "source_url": "https://reactome.org/content/detail/R-HSA-200512", "summary": "NMNAT1 transfers an adenylyl group from ATP to NAMN to yield NAAD is a Reactome release 97 curated reaction event. The source record connects NAMN, ATP, H+ to NAAD, PPi in nucleoplasm and cross-references RHEA:22861, RHEA:21361.", "equation": "NAMN + ATP + H+ → NAAD + PPi", "inputs": [ { "node_id": "R-ALL-200492", "reactome_id": "R-ALL-200492", "label": "NAMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57502", "formula": "C11H12NO9P", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-200499", "reactome_id": "R-ALL-200499", "label": "NAAD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58437", "formula": "C21H24N6O15P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113541", "reactome_id": "R-ALL-113541", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinate-nucleotide adenylyltransferase activity of 6xNMNAT1:6xZn2+ [nucleoplasm]", "activity_id": "200481", "ec_numbers": [ "2.7.7.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:16118205", "title": "Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16118205/" }, { "id": "PMID:17402747", "title": "Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17402747/" }, { "id": "PMID:11248244", "title": "Characterization of recombinant human nicotinamide mononucleotide adenylyl transferase (NMNAT), a nuclear enzyme essential for NAD synthesis", "journal": "FEBS Lett", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11248244/" }, { "id": "PMID:11788603", "title": "Structure of human nicotinamide/nicotinic acid mononucleotide adenylyltransferase. Basis for the dual substrate specificity and activation of the oncolytic agent tiazofurin.", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11788603/" } ], "x": 5670, "y": 2828.592105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200555", "label": "Formation of Malonyl-CoA from Acetyl-CoA (liver)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11309" ], "source_url": "https://reactome.org/content/detail/R-HSA-200555", "summary": "Formation of Malonyl-CoA from Acetyl-CoA (liver) is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, ATP, HCO3- to ADP, Pi, Mal-CoA in cytosol and cross-references RHEA:11309.", "equation": "Ac-CoA + ATP + HCO3- → ADP + Pi + Mal-CoA + H+", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111627", "reactome_id": "R-ALL-111627", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29508", "reactome_id": "R-ALL-29508", "label": "Mal-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acetyl-CoA carboxylase activity of Btn-ACACA [cytosol]", "activity_id": "200565", "ec_numbers": [ "6.4.1.2" ], "accessions": [ "Q13085" ] } ], "citations": [ { "id": "PMID:7732023", "title": "Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7732023/" }, { "id": "PMID:16854592", "title": "Expression, purification, and characterization of human and rat acetyl coenzyme A carboxylase (ACC) isozymes", "journal": "Protein Expr Purif", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/16854592/" }, { "id": "PMID:18455495", "title": "Differential activation of recombinant human acetyl-CoA carboxylases 1 and 2 by citrate", "journal": "Arch Biochem Biophys", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18455495/" } ], "x": 311.33333333333337, "y": 1643.140350877193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200644", "label": "5,10-methyleneTHF polyglutamate + NADP+ 5,10-methenylTHF polyglutamate + NADPH + H+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200644", "summary": "5,10-methyleneTHF polyglutamate + NADP+ 5,10-methenylTHF polyglutamate + NADPH + H+ is a Reactome release 97 curated reaction event. The source record connects 5,10-methylene-THFPG, NADP+ to 5,10-methenyl-THFPG, NADPH, H+ in cytosol.", "equation": "5,10-methylene-THFPG + NADP+ → 5,10-methenyl-THFPG + NADPH + H+", "inputs": [ { "node_id": "R-ALL-200739", "reactome_id": "R-ALL-200739", "label": "5,10-methylene-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60473", "formula": "(C5H6NO3)n.C15H15N6O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-200640", "reactome_id": "R-ALL-200640", "label": "5,10-methenyl-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65049", "formula": "(C5H6NO3)n.C25H26N8O9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "methylenetetrahydrofolate dehydrogenase (NADP+) activity of MTHFD1 dimer [cytosol]", "activity_id": "200717", "ec_numbers": [ "1.5.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:3871768", "title": "C1-Tetrahydrofolate synthase from rabbit liver. Structural and kinetic properties of the enzyme and its two domains.", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3871768/" }, { "id": "PMID:3053686", "title": "Primary structure of a human trifunctional enzyme. Isolation of a cDNA encoding methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase.", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3053686/" } ], "x": 5008.666666666667, "y": 2997.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200646", "label": "Cytosolic folate export across the plasma membrane", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:70159" ], "source_url": "https://reactome.org/content/detail/R-HSA-200646", "summary": "Cytosolic folate export across the plasma membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects FOLA, H+ to FOLA, H+ in plasma membrane and cross-references RHEA:70159.", "equation": "FOLA + H+ → FOLA + H+", "inputs": [ { "node_id": "R-ALL-197965", "reactome_id": "R-ALL-197965", "label": "FOLA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62501", "formula": "C19H17N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-200716", "reactome_id": "R-ALL-200716", "label": "FOLA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62501", "formula": "C19H17N7O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "folic acid:proton symporter activity of SLC46A1 [plasma membrane]", "activity_id": "200706", "ec_numbers": null, "accessions": [ "Q96NT5" ] } ], "citations": [ { "id": "PMID:8662720", "title": "Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662720/" }, { "id": "PMID:17129779", "title": "Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption", "journal": "Cell", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17129779/" }, { "id": "PMID:17962486", "title": "Cloning and functional characterization of the proton-coupled electrogenic folate transporter and analysis of its expression in retinal cell types", "journal": "Invest Ophthalmol Vis Sci", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17962486/" } ], "x": 4730, "y": 2856.0454545454545, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-200651", "label": "5,10-methyleneTHF polyglutamate + glycine tetrahydrofolate polyglutamate (THF polyglutamate) + serine", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200651", "summary": "5,10-methyleneTHF polyglutamate + glycine tetrahydrofolate polyglutamate (THF polyglutamate) + serine is a Reactome release 97 curated reaction event. The source record connects 5,10-methylene-THFPG, Gly to THFPG, L-Ser in cytosol.", "equation": "5,10-methylene-THFPG + Gly → THFPG + L-Ser", "inputs": [ { "node_id": "R-ALL-200739", "reactome_id": "R-ALL-200739", "label": "5,10-methylene-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60473", "formula": "(C5H6NO3)n.C15H15N6O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-197969", "reactome_id": "R-ALL-197969", "label": "THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28624", "formula": "(C5H7NO3)n.C19H23N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aldehyde-lyase activity of SHMT1 tetramer [cytosol]", "activity_id": "71248", "ec_numbers": [ "4.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9753690", "title": "The crystal structure of human cytosolic serine hydroxymethyltransferase: a target for cancer chemotherapy", "journal": "Structure", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9753690/" } ], "x": 5008.666666666667, "y": 3032.8636363636365, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200652", "label": "SLC19A1 transports 5-methyl-THF from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200652", "summary": "SLC19A1 transports 5-methyl-THF from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 5-methyl-THF to 5-methyl-THF in plasma membrane.", "equation": "5-methyl-THF → 5-methyl-THF", "inputs": [ { "node_id": "R-ALL-200709", "reactome_id": "R-ALL-200709", "label": "5-methyl-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18608", "formula": "C20H23N7O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-200665", "reactome_id": "R-ALL-200665", "label": "5-methyl-THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18608", "formula": "C20H23N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "folic acid transmembrane transporter activity of SLC19A1 [plasma membrane]", "activity_id": "200705", "ec_numbers": null, "accessions": [ "P41440" ] } ], "citations": [ { "id": "PMID:7852378", "title": "Isolation of a human cDNA that complements a mutant hamster cell defective in methotrexate uptake", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7852378/" }, { "id": "PMID:10347183", "title": "Topological and functional analysis of the human reduced folate carrier by hemagglutinin epitope insertion", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10347183/" } ], "x": 4730, "y": 3103.590909090909, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-200661", "label": "MTHFD1 dimer transforms 10-formyl-THFPG to 5,10-methenyl-THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200661", "summary": "MTHFD1 dimer transforms 10-formyl-THFPG to 5,10-methenyl-THFPG is a Reactome release 97 curated reaction event. The source record connects 10-formyl-THFPG to 5,10-methenyl-THFPG, H2O in cytosol.", "equation": "10-formyl-THFPG → 5,10-methenyl-THFPG + H2O", "inputs": [ { "node_id": "R-ALL-111353", "reactome_id": "R-ALL-111353", "label": "10-formyl-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28010", "formula": "(C5H7NO3)n.C25H30N8O10", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-200640", "reactome_id": "R-ALL-200640", "label": "5,10-methenyl-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65049", "formula": "(C5H6NO3)n.C25H26N8O9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "methenyltetrahydrofolate cyclohydrolase activity of MTHFD1 dimer [cytosol]", "activity_id": "200745", "ec_numbers": [ "3.5.4.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:3871768", "title": "C1-Tetrahydrofolate synthase from rabbit liver. Structural and kinetic properties of the enzyme and its two domains.", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3871768/" }, { "id": "PMID:3053686", "title": "Primary structure of a human trifunctional enzyme. Isolation of a cDNA encoding methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase.", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3053686/" } ], "x": 4730, "y": 3068.227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200676", "label": "MTHFR dimer reduces 5,10-methylene-THFPG to 5-methyl-THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200676", "summary": "MTHFR dimer reduces 5,10-methylene-THFPG to 5-methyl-THFPG is a Reactome release 97 curated reaction event. The source record connects 5,10-methylene-THFPG, NADPH, H+ to 5-methyl-THFPG, NADP+ in cytosol.", "equation": "5,10-methylene-THFPG + NADPH + H+ → 5-methyl-THFPG + NADP+", "inputs": [ { "node_id": "R-ALL-200739", "reactome_id": "R-ALL-200739", "label": "5,10-methylene-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60473", "formula": "(C5H6NO3)n.C15H15N6O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-174404", "reactome_id": "R-ALL-174404", "label": "5-methyl-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63907", "formula": "(C5H7NO3)n.C25H32N8O9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "methylenetetrahydrofolate reductase [NAD(P)H] activity of MTHFR dimer [cytosol]", "activity_id": "200678", "ec_numbers": [ "1.5.1.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:7726158", "title": "Seven novel mutations in the methylenetetrahydrofolate reductase gene and genotype/phenotype correlations in severe methylenetetrahydrofolate reductase deficiency", "journal": "Am J Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7726158/" } ], "x": 4451.333333333333, "y": 3032.8636363636365, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200680", "label": "SLC25A32 transports THF from cytosol to mitochondrial matrix", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200680", "summary": "SLC25A32 transports THF from cytosol to mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects THF to THF in mitochondrial inner membrane.", "equation": "THF → THF", "inputs": [ { "node_id": "R-ALL-111355", "reactome_id": "R-ALL-111355", "label": "THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-200671", "reactome_id": "R-ALL-200671", "label": "THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "folic acid transmembrane transporter activity of SLC25A32 [mitochondrial inner membrane]", "activity_id": "200687", "ec_numbers": null, "accessions": [ "Q9H2D1" ] } ], "citations": [ { "id": "PMID:10978331", "title": "Retrovirally mediated complementation of the glyB phenotype. Cloning of a human gene encoding the carrier for entry of folates into mitochondria.", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10978331/" }, { "id": "PMID:8662720", "title": "Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662720/" } ], "x": 5008.666666666667, "y": 3103.590909090909, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-200681", "label": "FPGS-2 transforms 5-methyl-THF to 5-methyl-THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200681", "summary": "FPGS-2 transforms 5-methyl-THF to 5-methyl-THFPG is a Reactome release 97 curated reaction event. The source record connects 5-methyl-THF, ATP, L-Glu to 5-methyl-THFPG, ADP, Pi in cytosol.", "equation": "5-methyl-THF + 5 ATP + 5 L-Glu → 5-methyl-THFPG + 5 ADP + 5 Pi", "inputs": [ { "node_id": "R-ALL-200665", "reactome_id": "R-ALL-200665", "label": "5-methyl-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18608", "formula": "C20H23N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 5, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 5, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-174404", "reactome_id": "R-ALL-174404", "label": "5-methyl-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63907", "formula": "(C5H7NO3)n.C25H32N8O9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 5, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 5, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "tetrahydrofolylpolyglutamate synthase activity of FPGS-2 [cytosol]", "activity_id": "197957", "ec_numbers": [ "6.3.2.17" ], "accessions": [ "Q05932" ] } ], "citations": [ { "id": "PMID:1409616", "title": "Expression cloning of a human cDNA encoding folylpoly(gamma-glutamate) synthetase and determination of its primary structure", "journal": "Proc Natl Acad Sci U S A", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1409616/" }, { "id": "PMID:8662720", "title": "Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662720/" } ], "x": 5008.666666666667, "y": 2926.7727272727275, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200682", "label": "Mitochondrial FPGS-1 transforms THF to THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200682", "summary": "Mitochondrial FPGS-1 transforms THF to THFPG is a Reactome release 97 curated reaction event. The source record connects THF, ATP, L-Glu to THFPG, ADP, Pi in mitochondrial matrix.", "equation": "THF + 6 ATP + 6 L-Glu → THFPG + 6 ADP + 6 Pi", "inputs": [ { "node_id": "R-ALL-200671", "reactome_id": "R-ALL-200671", "label": "THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 6, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "input", "stoichiometry": 6, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-200679", "reactome_id": "R-ALL-200679", "label": "THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28624", "formula": "(C5H7NO3)n.C19H23N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 6, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 6, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "tetrahydrofolylpolyglutamate synthase activity of FPGS-1 [mitochondrial matrix]", "activity_id": "200730", "ec_numbers": [ "6.3.2.17" ], "accessions": [ "Q05932" ] } ], "citations": [ { "id": "PMID:1409616", "title": "Expression cloning of a human cDNA encoding folylpoly(gamma-glutamate) synthetase and determination of its primary structure", "journal": "Proc Natl Acad Sci U S A", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1409616/" }, { "id": "PMID:8662720", "title": "Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662720/" } ], "x": 4451.333333333333, "y": 2962.1363636363635, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200711", "label": "MTHFD1 dimer ligates HCOOH to THFPG to form 10-formyl-THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200711", "summary": "MTHFD1 dimer ligates HCOOH to THFPG to form 10-formyl-THFPG is a Reactome release 97 curated reaction event. The source record connects THFPG, HCOOH, ATP to 10-formyl-THFPG, ADP, Pi in cytosol.", "equation": "THFPG + HCOOH + ATP → 10-formyl-THFPG + ADP + Pi", "inputs": [ { "node_id": "R-ALL-197969", "reactome_id": "R-ALL-197969", "label": "THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28624", "formula": "(C5H7NO3)n.C19H23N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111353", "reactome_id": "R-ALL-111353", "label": "10-formyl-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28010", "formula": "(C5H7NO3)n.C25H30N8O10", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "formate-tetrahydrofolate ligase activity of MTHFD1 dimer [cytosol]", "activity_id": "200692", "ec_numbers": [ "6.3.4.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:3871768", "title": "C1-Tetrahydrofolate synthase from rabbit liver. Structural and kinetic properties of the enzyme and its two domains.", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3871768/" }, { "id": "PMID:3053686", "title": "Primary structure of a human trifunctional enzyme. Isolation of a cDNA encoding methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase.", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3053686/" } ], "x": 4730, "y": 2962.1363636363635, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200718", "label": "MTHFD1 dimer dehydrogenates 5,10-methenyl-THFPG to 5,10-methylene-THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200718", "summary": "MTHFD1 dimer dehydrogenates 5,10-methenyl-THFPG to 5,10-methylene-THFPG is a Reactome release 97 curated reaction event. The source record connects 5,10-methenyl-THFPG, NADPH, H+ to 5,10-methylene-THFPG, NADP+ in cytosol.", "equation": "5,10-methenyl-THFPG + NADPH + H+ → 5,10-methylene-THFPG + NADP+", "inputs": [ { "node_id": "R-ALL-200640", "reactome_id": "R-ALL-200640", "label": "5,10-methenyl-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65049", "formula": "(C5H6NO3)n.C25H26N8O9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-200739", "reactome_id": "R-ALL-200739", "label": "5,10-methylene-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60473", "formula": "(C5H6NO3)n.C15H15N6O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "methylenetetrahydrofolate dehydrogenase (NADP+) activity of MTHFD1 dimer [cytosol]", "activity_id": "200717", "ec_numbers": [ "1.5.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:3871768", "title": "C1-Tetrahydrofolate synthase from rabbit liver. Structural and kinetic properties of the enzyme and its two domains.", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3871768/" }, { "id": "PMID:3053686", "title": "Primary structure of a human trifunctional enzyme. Isolation of a cDNA encoding methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase.", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3053686/" } ], "x": 4730, "y": 3032.8636363636365, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200720", "label": "SLC25A32 transports THF from mitochondrial matrix to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200720", "summary": "SLC25A32 transports THF from mitochondrial matrix to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects THF to THF in mitochondrial inner membrane.", "equation": "THF → THF", "inputs": [ { "node_id": "R-ALL-200671", "reactome_id": "R-ALL-200671", "label": "THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111355", "reactome_id": "R-ALL-111355", "label": "THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "folic acid transmembrane transporter activity of SLC25A32 [mitochondrial inner membrane]", "activity_id": "200687", "ec_numbers": null, "accessions": [ "Q9H2D1" ] } ], "citations": [ { "id": "PMID:10978331", "title": "Retrovirally mediated complementation of the glyB phenotype. Cloning of a human gene encoding the carrier for entry of folates into mitochondria.", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10978331/" }, { "id": "PMID:8662720", "title": "Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662720/" } ], "x": 4451.333333333333, "y": 3138.9545454545455, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-200729", "label": "SLC46A1 transports FOLA from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:70159" ], "source_url": "https://reactome.org/content/detail/R-HSA-200729", "summary": "SLC46A1 transports FOLA from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects FOLA, H+ to FOLA, H+ in plasma membrane and cross-references RHEA:70159.", "equation": "FOLA + H+ → FOLA + H+", "inputs": [ { "node_id": "R-ALL-200716", "reactome_id": "R-ALL-200716", "label": "FOLA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62501", "formula": "C19H17N7O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-197965", "reactome_id": "R-ALL-197965", "label": "FOLA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62501", "formula": "C19H17N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "folic acid:proton symporter activity of SLC46A1 [plasma membrane]", "activity_id": "200706", "ec_numbers": null, "accessions": [ "Q96NT5" ] } ], "citations": [ { "id": "PMID:8662720", "title": "Purification and properties of human cytosolic folylpoly-gamma-glutamate synthetase and organization, localization, and differential splicing of its gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8662720/" }, { "id": "PMID:17129779", "title": "Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption", "journal": "Cell", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17129779/" }, { "id": "PMID:17962486", "title": "Cloning and functional characterization of the proton-coupled electrogenic folate transporter and analysis of its expression in retinal cell types", "journal": "Invest Ophthalmol Vis Sci", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17962486/" } ], "x": 5008.666666666667, "y": 2856.0454545454545, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-200735", "label": "SHMT1 tetramer transfers hydroxymethyl group to THFPG to form 5,10-methylene-THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200735", "summary": "SHMT1 tetramer transfers hydroxymethyl group to THFPG to form 5,10-methylene-THFPG is a Reactome release 97 curated reaction event. The source record connects THFPG, L-Ser to 5,10-methylene-THFPG, Gly in cytosol.", "equation": "THFPG + L-Ser → 5,10-methylene-THFPG + Gly", "inputs": [ { "node_id": "R-ALL-197969", "reactome_id": "R-ALL-197969", "label": "THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28624", "formula": "(C5H7NO3)n.C19H23N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-200739", "reactome_id": "R-ALL-200739", "label": "5,10-methylene-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60473", "formula": "(C5H6NO3)n.C15H15N6O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aldehyde-lyase activity of SHMT1 tetramer [cytosol]", "activity_id": "71248", "ec_numbers": [ "4.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9753690", "title": "The crystal structure of human cytosolic serine hydroxymethyltransferase: a target for cancer chemotherapy", "journal": "Structure", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9753690/" } ], "x": 4451.333333333333, "y": 3068.227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-200740", "label": "5,10-methenylTHF polyglutamate + H2O 10-formylTHF polyglutamate", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-200740", "summary": "5,10-methenylTHF polyglutamate + H2O 10-formylTHF polyglutamate is a Reactome release 97 curated reaction event. The source record connects 5,10-methenyl-THFPG, H2O to 10-formyl-THFPG in cytosol.", "equation": "5,10-methenyl-THFPG + H2O → 10-formyl-THFPG", "inputs": [ { "node_id": "R-ALL-200640", "reactome_id": "R-ALL-200640", "label": "5,10-methenyl-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65049", "formula": "(C5H6NO3)n.C25H26N8O9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111353", "reactome_id": "R-ALL-111353", "label": "10-formyl-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28010", "formula": "(C5H7NO3)n.C25H30N8O10", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methenyltetrahydrofolate cyclohydrolase activity of MTHFD1 dimer [cytosol]", "activity_id": "200745", "ec_numbers": [ "3.5.4.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:3871768", "title": "C1-Tetrahydrofolate synthase from rabbit liver. Structural and kinetic properties of the enzyme and its two domains.", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3871768/" }, { "id": "PMID:3053686", "title": "Primary structure of a human trifunctional enzyme. Isolation of a cDNA encoding methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase.", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3053686/" } ], "x": 5008.666666666667, "y": 3068.227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-201035", "label": "ACSL1,3,5,6 ligate CoA to PALM to form PALM-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:30752" ], "source_url": "https://reactome.org/content/detail/R-HSA-201035", "summary": "ACSL1,3,5,6 ligate CoA to PALM to form PALM-CoA is a Reactome release 97 curated reaction event. The source record connects ATP, CoA-SH, PALM to AMP, H2O, PALM-CoA in cytosol · endoplasmic reticulum membrane and cross-references RHEA:30752.", "equation": "ATP + CoA-SH + PALM → AMP + H2O + PALM-CoA + PPi", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-3632881", "reactome_id": "R-ALL-3632881", "label": "PALM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:7896", "formula": "C16H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-200993", "reactome_id": "R-ALL-200993", "label": "PALM-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57379", "formula": "C37H62N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of ACSL1,3,5,6 [endoplasmic reticulum membrane]", "activity_id": "200992", "ec_numbers": [ "6.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:10548543", "title": "Identification and molecular characterization of acyl-CoA synthetase in human erythrocytes and erythroid precursors", "journal": "Biochem J", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10548543/" }, { "id": "PMID:17379924", "title": "Involvement of ACSL in local synthesis of neutral lipids in cytoplasmic lipid droplets in human hepatocyte HuH7", "journal": "J Lipid Res", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17379924/" }, { "id": "PMID:17681178", "title": "Regulation of enterocyte apoptosis by acyl-CoA synthetase 5 splicing", "journal": "Gastroenterology", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17681178/" } ], "x": 590, "y": 1643.140350877193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2018659", "label": "CHST15 transfers sulfate to (6-)GalNAc-4-sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:54301" ], "source_url": "https://reactome.org/content/detail/R-HSA-2018659", "summary": "CHST15 transfers sulfate to (6-)GalNAc-4-sulfate is a Reactome release 97 curated reaction event. The source record connects C4S-PG, PAPS to PAP, H+, CSE-PG in Golgi membrane · Golgi lumen and cross-references RHEA:54301.", "equation": "C4S-PG + PAPS → PAP + H+ + CSE-PG", "inputs": [ { "node_id": "R-HSA-2064226", "reactome_id": "R-HSA-2064226", "label": "C4S-PG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2064101", "reactome_id": "R-HSA-2064101", "label": "CSE-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity of CHST15 [Golgi membrane]", "activity_id": "2018675", "ec_numbers": [ "2.8.2.33" ], "accessions": [ "Q7LFX5" ] } ], "citations": [ { "id": "PMID:12874280", "title": "A unique nonreducing terminal modification of chondroitin sulfate by N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12874280/" } ], "x": 2660, "y": 818.6212121212121, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2018682", "label": "CHST3,7 transfer SO4(2-) to position 6 of GalNAc on chondroitin chains", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:11109" ], "source_url": "https://reactome.org/content/detail/R-HSA-2018682", "summary": "CHST3,7 transfer SO4(2-) to position 6 of GalNAc on chondroitin chains is a Reactome release 97 curated reaction event. The source record connects chondroitin(3)-core proteins, PAPS to PAP, C6S-PG, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:11109.", "equation": "chondroitin(3)-core proteins + PAPS → PAP + C6S-PG + H+", "inputs": [ { "node_id": "R-HSA-2064075", "reactome_id": "R-HSA-2064075", "label": "chondroitin(3)-core proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064219", "reactome_id": "R-HSA-2064219", "label": "C6S-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "chondroitin 6-sulfotransferase activity of CHST3,7 [Golgi membrane]", "activity_id": "2018681", "ec_numbers": [ "2.8.2.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:9883891", "title": "Functional expression and genomic structure of human chondroitin 6-sulfotransferase", "journal": "FEBS Lett", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9883891/" }, { "id": "PMID:10781596", "title": "Molecular cloning and expression of a novel chondroitin 6-O-sulfotransferase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10781596/" } ], "x": 2938.666666666667, "y": 818.6212121212121, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2022052", "label": "DSE converts GlcA to IdoA", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:21085" ], "source_url": "https://reactome.org/content/detail/R-HSA-2022052", "summary": "DSE converts GlcA to IdoA is a Reactome release 97 curated reaction event. The source record connects DSE,DSEL substrates to DSE,DSEL products in Golgi membrane · Golgi lumen and cross-references RHEA:21085.", "equation": "DSE,DSEL substrates → DSE,DSEL products", "inputs": [ { "node_id": "R-HSA-5607547", "reactome_id": "R-HSA-5607547", "label": "DSE,DSEL substrates", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5607545", "reactome_id": "R-HSA-5607545", "label": "DSE,DSEL products", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "chondroitin-glucuronate 5-epimerase activity of DSE,DSEL [Golgi membrane]", "activity_id": "2022049", "ec_numbers": [ "5.1.3.19" ], "accessions": [] } ], "citations": [ { "id": "PMID:16505484", "title": "Biosynthesis of dermatan sulfate: chondroitin-glucuronate C5-epimerase is identical to SART2", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16505484/" }, { "id": "PMID:11414686", "title": "The glucuronyl C5-epimerase activity is the limiting factor in the dermatan sulfate biosynthesis", "journal": "Arch Biochem Biophys", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11414686/" }, { "id": "PMID:12556911", "title": "A novel CpG-associated brain-expressed candidate gene for chromosome 18q-linked bipolar disorder", "journal": "Mol. Psychiatry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12556911/" }, { "id": "PMID:19188366", "title": "Two dermatan sulfate epimerases form iduronic acid domains in dermatan sulfate", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19188366/" }, { "id": "PMID:23704329", "title": "Loss of dermatan sulfate epimerase (DSE) function results in musculocontractural Ehlers-Danlos syndrome", "journal": "Hum Mol Genet", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23704329/" } ], "x": 2938.666666666667, "y": 901.1363636363636, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2022056", "label": "DSPG and CSPG translocate to the lysosome for degradation", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "extracellular region · lysosomal lumen", "compartment_ids": [ "GO:0005576", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2022056", "summary": "DSPG and CSPG translocate to the lysosome for degradation is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CSPGs,DSPGs to CSPGs,DSPGs in extracellular region · lysosomal lumen.", "equation": "CSPGs,DSPGs → CSPGs,DSPGs", "inputs": [ { "node_id": "R-HSA-9941288", "reactome_id": "R-HSA-9941288", "label": "CSPGs,DSPGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9941002", "reactome_id": "R-HSA-9941002", "label": "CSPGs,DSPGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15647514", "title": "Lysosomal metabolism of glycoproteins", "journal": "Glycobiology", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15647514/" } ], "x": 2660, "y": 1089.7424242424242, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2022061", "label": "Dermatan sulfate can be further sulfated on position 2 of iduronate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2022061", "summary": "Dermatan sulfate can be further sulfated on position 2 of iduronate is a Reactome release 97 curated reaction event. The source record connects D4S-PGs, PAPS to PAP, D2,4(S)2-PG, H+ in Golgi membrane · Golgi lumen.", "equation": "D4S-PGs + PAPS → PAP + D2,4(S)2-PG + H+", "inputs": [ { "node_id": "R-HSA-2065135", "reactome_id": "R-HSA-2065135", "label": "D4S-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2065145", "reactome_id": "R-HSA-2065145", "label": "D2,4(S)2-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sulfotransferase activity of UST [Golgi membrane]", "activity_id": "2022062", "ec_numbers": [ "2.8.2" ], "accessions": [ "Q9Y2C2" ] } ], "citations": [ { "id": "PMID:10187838", "title": "Molecular cloning and characterization of a human uronyl 2-sulfotransferase that sulfates iduronyl and glucuronyl residues in dermatan/chondroitin sulfate", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10187838/" } ], "x": 2938.666666666667, "y": 995.439393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2022063", "label": "CHST14 transfers SO4(2-) to GalNAc in dermatan or DS", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:48053" ], "source_url": "https://reactome.org/content/detail/R-HSA-2022063", "summary": "CHST14 transfers SO4(2-) to GalNAc in dermatan or DS is a Reactome release 97 curated reaction event. The source record connects CHST14 substrates, PAPS to PAP, CHST14 products, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:48053.", "equation": "CHST14 substrates + PAPS → PAP + CHST14 products + H+", "inputs": [ { "node_id": "R-HSA-5607518", "reactome_id": "R-HSA-5607518", "label": "CHST14 substrates", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-5607524", "reactome_id": "R-HSA-5607524", "label": "CHST14 products", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dermatan 4-sulfotransferase activity of CHST14 [Golgi membrane]", "activity_id": "2022068", "ec_numbers": [ "2.8.2.35" ], "accessions": [ "Q8NCH0" ] } ], "citations": [ { "id": "PMID:19661164", "title": "Dermatan 4-O-sulfotransferase 1 is pivotal in the formation of iduronic acid blocks in dermatan sulfate", "journal": "Glycobiology", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19661164/" }, { "id": "PMID:11470797", "title": "Molecular cloning and characterization of a dermatan-specific N-acetylgalactosamine 4-O-sulfotransferase", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11470797/" }, { "id": "PMID:20533528", "title": "Loss-of-function mutations of CHST14 in a new type of Ehlers-Danlos syndrome", "journal": "Hum. Mutat.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20533528/" } ], "x": 2381.3333333333335, "y": 830.4090909090909, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2022065", "label": "DSPGs are secreted", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi lumen · extracellular region", "compartment_ids": [ "GO:0005796", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2022065", "summary": "DSPGs are secreted is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DSPGs to DSPGs in Golgi lumen · extracellular region.", "equation": "DSPGs → DSPGs", "inputs": [ { "node_id": "R-HSA-2065267", "reactome_id": "R-HSA-2065267", "label": "DSPGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2065240", "reactome_id": "R-HSA-2065240", "label": "DSPGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12512856", "title": "Biosynthesis of chondroitin/dermatan sulfate", "journal": "IUBMB Life", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12512856/" } ], "x": 2938.666666666667, "y": 1089.7424242424242, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2022851", "label": "EXT1:EXT2 transfers GlcNAc to the heparan chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:16214" ], "source_url": "https://reactome.org/content/detail/R-HSA-2022851", "summary": "EXT1:EXT2 transfers GlcNAc to the heparan chain is a Reactome release 97 curated reaction event. The source record connects UDP-GlcNAc, Heparan(1)-PGs to UDP, Heparan(2)-PGs, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:16214.", "equation": "UDP-GlcNAc + Heparan(1)-PGs → UDP + Heparan(2)-PGs + H+", "inputs": [ { "node_id": "R-ALL-914003", "reactome_id": "R-ALL-914003", "label": "UDP-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076416", "reactome_id": "R-HSA-2076416", "label": "Heparan(1)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076412", "reactome_id": "R-HSA-2076412", "label": "Heparan(2)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucuronosyl-N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity of EXT1:EXT2 [Golgi membrane]", "activity_id": "2022845", "ec_numbers": [ "2.4.1.224" ], "accessions": [] } ], "citations": [ { "id": "PMID:11169766", "title": "Diminished levels of the putative tumor suppressor proteins EXT1 and EXT2 in exostosis chondrocytes", "journal": "Cell Motil Cytoskeleton", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11169766/" }, { "id": "PMID:9463333", "title": "Mutations in the EXT1 and EXT2 genes in hereditary multiple exostoses", "journal": "Am J Hum Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9463333/" }, { "id": "PMID:10639137", "title": "The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10639137/" } ], "x": 2660, "y": 830.4090909090909, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2022856", "label": "EXT1:EXT2 transfers GlcA to heparan", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:20909" ], "source_url": "https://reactome.org/content/detail/R-HSA-2022856", "summary": "EXT1:EXT2 transfers GlcA to heparan is a Reactome release 97 curated reaction event. The source record connects UDP-GlcA, Heparan-PGs to UDP, Heparan(1)-PGs, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:20909.", "equation": "UDP-GlcA + Heparan-PGs → UDP + Heparan(1)-PGs + H+", "inputs": [ { "node_id": "R-ALL-1889970", "reactome_id": "R-ALL-1889970", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076465", "reactome_id": "R-HSA-2076465", "label": "Heparan-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076416", "reactome_id": "R-HSA-2076416", "label": "Heparan(1)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity of EXT1:EXT2 [Golgi membrane]", "activity_id": "2022849", "ec_numbers": [ "2.4.1.225" ], "accessions": [] } ], "citations": [ { "id": "PMID:10639137", "title": "The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10639137/" }, { "id": "PMID:11169766", "title": "Diminished levels of the putative tumor suppressor proteins EXT1 and EXT2 in exostosis chondrocytes", "journal": "Cell Motil Cytoskeleton", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11169766/" }, { "id": "PMID:9463333", "title": "Mutations in the EXT1 and EXT2 genes in hereditary multiple exostoses", "journal": "Am J Hum Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9463333/" } ], "x": 2938.666666666667, "y": 830.4090909090909, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2022860", "label": "NDST1-4 can sulfate a glucosamine residue in heparan to form heparan sulfate (HS)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:21981" ], "source_url": "https://reactome.org/content/detail/R-HSA-2022860", "summary": "NDST1-4 can sulfate a glucosamine residue in heparan to form heparan sulfate (HS) is a Reactome release 97 curated reaction event. The source record connects PAPS, Heparan(4)-PGs to PAP, HS(1)-PGs, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:21981.", "equation": "2 PAPS + Heparan(4)-PGs → 2 PAP + HS(1)-PGs + H+", "inputs": [ { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076346", "reactome_id": "R-HSA-2076346", "label": "Heparan(4)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076452", "reactome_id": "R-HSA-2076452", "label": "HS(1)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "heparan sulfate N-sulfotransferase activity of NDSTs [Golgi membrane]", "activity_id": "2022920", "ec_numbers": [ "2.8.2.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:16343444", "title": "Characterization of the N-deacetylase domain from the heparan sulfate N-deacetylase/N-sulfotransferase 2", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16343444/" }, { "id": "PMID:7601448", "title": "Cloning of the human heparan sulfate-N-deacetylase/N-sulfotransferase gene from the Treacher Collins syndrome candidate region at 5q32-q33.1", "journal": "Genomics", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7601448/" }, { "id": "PMID:9915799", "title": "Molecular cloning and expression of a third member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9915799/" }, { "id": "PMID:11087757", "title": "Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase. Structure and activity of the fourth member, NDST4", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11087757/" } ], "x": 2381.3333333333335, "y": 842.1969696969697, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2022887", "label": "NDST1-4 N-deacetylates GlcNAc residues in heparan", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:70588" ], "source_url": "https://reactome.org/content/detail/R-HSA-2022887", "summary": "NDST1-4 N-deacetylates GlcNAc residues in heparan is a Reactome release 97 curated reaction event. The source record connects H2O, Heparan(3)-PGs to CH3COO-, Heparan(4)-PGs in Golgi membrane · Golgi lumen and cross-references RHEA:70588.", "equation": "H2O + Heparan(3)-PGs → CH3COO- + Heparan(4)-PGs", "inputs": [ { "node_id": "R-ALL-2022884", "reactome_id": "R-ALL-2022884", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2076535", "reactome_id": "R-HSA-2076535", "label": "Heparan(3)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2022890", "reactome_id": "R-ALL-2022890", "label": "CH3COO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076346", "reactome_id": "R-HSA-2076346", "label": "Heparan(4)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "N-acetylglucosamine deacetylase activity of NDSTs [Golgi membrane]", "activity_id": "2022873", "ec_numbers": [ "3.5.1.33" ], "accessions": [] } ], "citations": [ { "id": "PMID:7601448", "title": "Cloning of the human heparan sulfate-N-deacetylase/N-sulfotransferase gene from the Treacher Collins syndrome candidate region at 5q32-q33.1", "journal": "Genomics", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7601448/" }, { "id": "PMID:16343444", "title": "Characterization of the N-deacetylase domain from the heparan sulfate N-deacetylase/N-sulfotransferase 2", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16343444/" }, { "id": "PMID:9915799", "title": "Molecular cloning and expression of a third member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9915799/" }, { "id": "PMID:11087757", "title": "Multiple isozymes of heparan sulfate/heparin GlcNAc N-deacetylase/GlcN N-sulfotransferase. Structure and activity of the fourth member, NDST4", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11087757/" } ], "x": 2938.666666666667, "y": 889.3484848484849, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2022911", "label": "CSPG is secreted", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi lumen · extracellular region", "compartment_ids": [ "GO:0005796", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2022911", "summary": "CSPG is secreted is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CSPGs to CSPGs in Golgi lumen · extracellular region.", "equation": "CSPGs → CSPGs", "inputs": [ { "node_id": "R-HSA-2024088", "reactome_id": "R-HSA-2024088", "label": "CSPGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2064124", "reactome_id": "R-HSA-2064124", "label": "CSPGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10963998", "title": "Biosynthesis of decorin and glypican", "journal": "Matrix Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10963998/" } ], "x": 2381.3333333333335, "y": 1101.530303030303, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2023971", "label": "1/3 PP-IP5 is dephosphorylated to IP6 by NUDT(1) in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2023971", "summary": "1/3 PP-IP5 is dephosphorylated to IP6 by NUDT(1) in the cytosol is a Reactome release 97 curated reaction event. The source record connects 1/3-PP-IP5, H2O to IP6, Pi in cytosol.", "equation": "1/3-PP-IP5 + H2O → IP6 + Pi", "inputs": [ { "node_id": "R-ALL-2023974", "reactome_id": "R-ALL-2023974", "label": "1/3-PP-IP5", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1629770", "reactome_id": "R-ALL-1629770", "label": "IP6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58130", "formula": "C6H6O24P6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol diphosphate pentakisphosphate diphosphatase activity of NUDT(1) [cytosol]", "activity_id": "2023976", "ec_numbers": [ "3.6.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:10777568", "title": "Discovery of molecular and catalytic diversity among human diphosphoinositol-polyphosphate phosphohydrolases. An expanding Nudt family", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777568/" }, { "id": "PMID:12121577", "title": "Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases", "journal": "BMC Biochem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12121577/" }, { "id": "PMID:12105228", "title": "An adjacent pair of human NUDT genes on chromosome X are preferentially expressed in testis and encode two new isoforms of diphosphoinositol polyphosphate phosphohydrolase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12105228/" }, { "id": "PMID:10419486", "title": "The diadenosine hexaphosphate hydrolases from Schizosaccharomyces pombe and Saccharomyces cerevisiae are homologues of the human diphosphoinositol polyphosphate phosphohydrolase. Overlapping substrate specificities in a MutT-type protein", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419486/" }, { "id": "PMID:9822604", "title": "A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase", "journal": "EMBO J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9822604/" }, { "id": "PMID:10585413", "title": "Site-directed mutagenesis of diphosphoinositol polyphosphate phosphohydrolase, a dual specificity NUDT enzyme that attacks diadenosine polyphosphates and diphosphoinositol polyphosphates", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10585413/" } ], "x": 7461.333333333333, "y": 1025.6458333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2023973", "label": "(PP)2-IP4 is dephosphorylated to 1/3-PP-IP5 by NUDT(1) in the cytosol", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2023973", "summary": "(PP)2-IP4 is dephosphorylated to 1/3-PP-IP5 by NUDT(1) in the cytosol is a Reactome release 97 curated reaction event. The source record connects (PP)2-IP4, H2O to 1/3-PP-IP5, Pi in cytosol.", "equation": "(PP)2-IP4 + H2O → 1/3-PP-IP5 + Pi", "inputs": [ { "node_id": "R-ALL-2023968", "reactome_id": "R-ALL-2023968", "label": "(PP)2-IP4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2023974", "reactome_id": "R-ALL-2023974", "label": "1/3-PP-IP5", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol bisdiphosphate tetrakisphosphate diphosphatase activity of NUDT(1) [cytosol]", "activity_id": "2023955", "ec_numbers": [ "3.6.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:12105228", "title": "An adjacent pair of human NUDT genes on chromosome X are preferentially expressed in testis and encode two new isoforms of diphosphoinositol polyphosphate phosphohydrolase", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12105228/" }, { "id": "PMID:10419486", "title": "The diadenosine hexaphosphate hydrolases from Schizosaccharomyces pombe and Saccharomyces cerevisiae are homologues of the human diphosphoinositol polyphosphate phosphohydrolase. Overlapping substrate specificities in a MutT-type protein", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419486/" }, { "id": "PMID:9822604", "title": "A novel context for the 'MutT' module, a guardian of cell integrity, in a diphosphoinositol polyphosphate phosphohydrolase", "journal": "EMBO J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9822604/" }, { "id": "PMID:10585413", "title": "Site-directed mutagenesis of diphosphoinositol polyphosphate phosphohydrolase, a dual specificity NUDT enzyme that attacks diadenosine polyphosphates and diphosphoinositol polyphosphates", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10585413/" }, { "id": "PMID:10777568", "title": "Discovery of molecular and catalytic diversity among human diphosphoinositol-polyphosphate phosphohydrolases. An expanding Nudt family", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777568/" }, { "id": "PMID:12121577", "title": "Cloning and characterisation of hAps1 and hAps2, human diadenosine polyphosphate-metabolising Nudix hydrolases", "journal": "BMC Biochem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12121577/" } ], "x": 7740, "y": 1090.4791666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2024084", "label": "HS-GAGs translocate to the lysosome for degradation", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane · lysosomal lumen", "compartment_ids": [ "GO:0005886", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2024084", "summary": "HS-GAGs translocate to the lysosome for degradation is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects HS/HPIN-PGs to HS/HPIN-PGs in plasma membrane · lysosomal lumen.", "equation": "HS/HPIN-PGs → HS/HPIN-PGs", "inputs": [ { "node_id": "R-HSA-2076639", "reactome_id": "R-HSA-2076639", "label": "HS/HPIN-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2090050", "reactome_id": "R-HSA-2090050", "label": "HS/HPIN-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15647514", "title": "Lysosomal metabolism of glycoproteins", "journal": "Glycobiology", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15647514/" }, { "id": "PMID:20576607", "title": "Heparanase 2 interacts with heparan sulfate with high affinity and inhibits heparanase activity", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20576607/" } ], "x": 2660, "y": 1101.530303030303, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2024100", "label": "GLCE epimerises GlcA to IdoA", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:20198" ], "source_url": "https://reactome.org/content/detail/R-HSA-2024100", "summary": "GLCE epimerises GlcA to IdoA is a Reactome release 97 curated reaction event. The source record connects HS(1)-PGs to HS(2)-PGs in Golgi membrane · Golgi lumen and cross-references RHEA:20198.", "equation": "HS(1)-PGs → HS(2)-PGs", "inputs": [ { "node_id": "R-HSA-2076452", "reactome_id": "R-HSA-2076452", "label": "HS(1)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2076425", "reactome_id": "R-HSA-2076425", "label": "HS(2)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "heparosan-N-sulfate-glucuronate 5-epimerase activity of GLCE dimer [Golgi membrane]", "activity_id": "2024110", "ec_numbers": [ "5.1.3.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:11274177", "title": "Characterization of the D-glucuronyl C5-epimerase involved in the biosynthesis of heparin and heparan sulfate", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11274177/" }, { "id": "PMID:11279150", "title": "Cloning, Golgi localization, and enzyme activity of the full-length heparin/heparan sulfate-glucuronic acid C5-epimerase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11279150/" } ], "x": 2381.3333333333335, "y": 912.9242424242424, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2024108", "label": "Some HSPGs are secreted to the plasma membrane", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi lumen · plasma membrane", "compartment_ids": [ "GO:0005796", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2024108", "summary": "Some HSPGs are secreted to the plasma membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects HSPGs to HSPGs in Golgi lumen · plasma membrane.", "equation": "HSPGs → HSPGs", "inputs": [ { "node_id": "R-HSA-2076619", "reactome_id": "R-HSA-2076619", "label": "HSPGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2076618", "reactome_id": "R-HSA-2076618", "label": "HSPGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10716625", "title": "Heparan sulfate proteoglycans on the cell surface: versatile coordinators of cellular functions", "journal": "Int J Biochem Cell Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10716625/" } ], "x": 2938.666666666667, "y": 1101.530303030303, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2025723", "label": "B4GALTs transfer Gal to the N-glycan precursor", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:22933" ], "source_url": "https://reactome.org/content/detail/R-HSA-2025723", "summary": "B4GALTs transfer Gal to the N-glycan precursor is a Reactome release 97 curated reaction event. The source record connects UDP-Gal, N-glycan-protein to UDP, Gal-glycan-protein, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:22933.", "equation": "UDP-Gal + N-glycan-protein → UDP + Gal-glycan-protein + H+", "inputs": [ { "node_id": "R-ALL-735682", "reactome_id": "R-ALL-735682", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2046280", "reactome_id": "R-HSA-2046280", "label": "N-glycan-protein", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2046271", "reactome_id": "R-HSA-2046271", "label": "Gal-glycan-protein", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity of B4GALT1-6 homodimers [Golgi membrane]", "activity_id": "975894", "ec_numbers": [ "2.4.1.38" ], "accessions": [] } ], "citations": [ { "id": "PMID:11588157", "title": "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells", "journal": "Glycobiology", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11588157/" }, { "id": "PMID:9597550", "title": "The expanding beta 4-galactosyltransferase gene family: messages from the databanks", "journal": "Glycobiology", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9597550/" }, { "id": "PMID:11901181", "title": "Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId", "journal": "J Clin Invest", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11901181/" }, { "id": "PMID:1714903", "title": "Evidence for a molecular distinction between Golgi and cell surface forms of beta 1,4-galactosyltransferase", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1714903/" }, { "id": "PMID:17021253", "title": "Transition of galactosyltransferase 1 from trans-Golgi cisterna to the trans-Golgi network is signal mediated", "journal": "Mol Biol Cell", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17021253/" }, { "id": "PMID:38034954", "title": "Exploiting Substrate Specificities of 6- O -Sulfotransferases to Enzymatically Synthesize Keratan Sulfate Oligosaccharides", "journal": "JACS Au", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/38034954/" } ], "x": 2660, "y": 842.1969696969697, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2025724", "label": "B3GNT1,2,3,4,7 add GlcNAc to form Keratan-PG", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:14390" ], "source_url": "https://reactome.org/content/detail/R-HSA-2025724", "summary": "B3GNT1,2,3,4,7 add GlcNAc to form Keratan-PG is a Reactome release 97 curated reaction event. The source record connects UDP-GlcNAc, Gal-glycan-protein to UDP, Keratan(1)-PG, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:14390.", "equation": "UDP-GlcNAc + Gal-glycan-protein → UDP + Keratan(1)-PG + H+", "inputs": [ { "node_id": "R-ALL-914003", "reactome_id": "R-ALL-914003", "label": "UDP-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2046271", "reactome_id": "R-HSA-2046271", "label": "Gal-glycan-protein", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2046187", "reactome_id": "R-HSA-2046187", "label": "Keratan(1)-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity of B3GNT1,2,3,4,7 [Golgi membrane]", "activity_id": "2046204", "ec_numbers": [ "2.4.1.149" ], "accessions": [] } ], "citations": [ { "id": "PMID:11042166", "title": "Identification and characterization of three novel beta 1,3-N-acetylglucosaminyltransferases structurally related to the beta 1,3-galactosyltransferase family", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11042166/" }, { "id": "PMID:9417100", "title": "Cloning of a human UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase catalyzing the formation of type 1 chains", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9417100/" }, { "id": "PMID:11283017", "title": "Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11283017/" }, { "id": "PMID:11821425", "title": "Molecular cloning and characterization of a novel UDP-GlcNAc:GalNAc-peptide beta1,3-N-acetylglucosaminyltransferase (beta 3Gn-T6), an enzyme synthesizing the core 3 structure of O-glycans", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11821425/" }, { "id": "PMID:15486459", "title": "Cloning and tissue distribution of the human B3GALT7 gene, a member of the beta1,3-Glycosyltransferase family", "journal": "Glycoconj J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15486459/" }, { "id": "PMID:15620693", "title": "A novel beta1,3-N-acetylglucosaminyltransferase (beta3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer", "journal": "FEBS Lett", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15620693/" } ], "x": 2938.666666666667, "y": 842.1969696969697, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-203946", "label": "PDK isozymes phosphorylate PDHC subunit E1", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:23053" ], "source_url": "https://reactome.org/content/detail/R-HSA-203946", "summary": "PDK isozymes phosphorylate PDHC subunit E1 is a Reactome release 97 curated reaction event. The source record connects PDH complex, ATP to p-lipo-PDH, ADP, H+ in mitochondrial matrix and cross-references RHEA:23053.", "equation": "PDH complex + 2 ATP → p-lipo-PDH + 2 ADP + H+", "inputs": [ { "node_id": "R-HSA-70070", "reactome_id": "R-HSA-70070", "label": "PDH complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-210342", "reactome_id": "R-HSA-210342", "label": "p-lipo-PDH", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyruvate dehydrogenase (acetyl-transferring) kinase activity of PDK isozyme dimers [mitochondrial matrix]", "activity_id": "203942", "ec_numbers": [ "2.7.11.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9405293", "title": "Evidence for existence of tissue-specific regulation of the mammalian pyruvate dehydrogenase complex", "journal": "Biochem J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9405293/" }, { "id": "PMID:7499431", "title": "Diversity of the pyruvate dehydrogenase kinase gene family in humans", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7499431/" }, { "id": "PMID:11485553", "title": "Regulation of pyruvate dehydrogenase activity through phosphorylation at multiple sites", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11485553/" }, { "id": "PMID:11486000", "title": "Site specificity of four pyruvate dehydrogenase kinase isoenzymes toward the three phosphorylation sites of human pyruvate dehydrogenase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11486000/" }, { "id": "PMID:8798399", "title": "Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8798399/" }, { "id": "PMID:33739396", "title": "Pyruvate dehydrogenase kinases (PDKs): an overview toward clinical applications", "journal": "Biosci Rep", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33739396/" } ], "x": 4730, "y": 438.04999999999995, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-204169", "label": "PDP1,2 dephosphorylate p-lipo-PDH", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12670" ], "source_url": "https://reactome.org/content/detail/R-HSA-204169", "summary": "PDP1,2 dephosphorylate p-lipo-PDH is a Reactome release 97 curated reaction event. The source record connects H2O, p-lipo-PDH to PDH complex, Pi in mitochondrial matrix and cross-references RHEA:12670.", "equation": "2 H2O + p-lipo-PDH → PDH complex + 2 Pi", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-210342", "reactome_id": "R-HSA-210342", "label": "p-lipo-PDH", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-70070", "reactome_id": "R-HSA-70070", "label": "PDH complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "[pyruvate dehydrogenase (acetyl-transferring)]-phosphatase activity of PDP [mitochondrial matrix]", "activity_id": "204160", "ec_numbers": [ "3.1.3.43" ], "accessions": [] } ], "citations": [ { "id": "PMID:9651365", "title": "Isoenzymes of pyruvate dehydrogenase phosphatase. DNA-derived amino acid sequences, expression, and regulation", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9651365/" }, { "id": "PMID:9395502", "title": "Cloning, expression, and properties of the regulatory subunit of bovine pyruvate dehydrogenase phosphatase", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9395502/" }, { "id": "PMID:15855260", "title": "Pyruvate dehydrogenase phosphatase deficiency: identification of the first mutation in two brothers and restoration of activity by protein complementation", "journal": "J Clin Endocrinol Metab", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15855260/" }, { "id": "PMID:17532339", "title": "Crystal structure of pyruvate dehydrogenase phosphatase 1 and its functional implications", "journal": "J Mol Biol", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17532339/" }, { "id": "PMID:16574315", "title": "Pyruvate dehydrogenase phosphatase deficiency: orphan disease or an under-diagnosed condition?", "journal": "Mol Cell Endocrinol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16574315/" } ], "x": 4451.333333333333, "y": 515.85, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046083", "label": "Elongation of arachidonyl-CoA to docosatetraenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046083", "summary": "Elongation of arachidonyl-CoA to docosatetraenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects AA-CoA, Malonyl-CoA, NADPH to DTA-CoA, CO2, CoA-SH in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "AA-CoA + Malonyl-CoA + 2 NADPH → DTA-CoA + CO2 + CoA-SH + H2O", "inputs": [ { "node_id": "R-ALL-2046053", "reactome_id": "R-ALL-2046053", "label": "AA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57368", "formula": "C41H62N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046066", "reactome_id": "R-ALL-2046066", "label": "Malonyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046060", "reactome_id": "R-ALL-2046060", "label": "DTA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63544", "formula": "C43H70N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159751", "reactome_id": "R-ALL-159751", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL5/ELOVL2 [endoplasmic reticulum membrane]", "activity_id": "2046081", "ec_numbers": [ "2.3.1.199" ], "accessions": [] } ], "citations": [ { "id": "PMID:10970790", "title": "Cloning of a human cDNA encoding a novel enzyme involved in the elongation of long-chain polyunsaturated fatty acids", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10970790/" } ], "x": 868.6666666666667, "y": 1697.4561403508771, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046084", "label": "Desaturation of alpha-linoleoyl-CoA to Stearidonoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046084", "summary": "Desaturation of alpha-linoleoyl-CoA to Stearidonoyl-CoA is a Reactome release 97 curated reaction event. The source record connects ALA-CoA, O2, NADH to SDA-CoA, H2O, NAD+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "ALA-CoA + O2 + 2 NADH → SDA-CoA + 2 H2O + 2 NAD+", "inputs": [ { "node_id": "R-ALL-2046062", "reactome_id": "R-ALL-2046062", "label": "ALA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:51985", "formula": "C39H64N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194697", "reactome_id": "R-ALL-194697", "label": "NADH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046052", "reactome_id": "R-ALL-2046052", "label": "SDA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63545", "formula": "C39H62N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-194653", "reactome_id": "R-ALL-194653", "label": "NAD+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyl-CoA 6-desaturase activity of FADS2 [endoplasmic reticulum membrane]", "activity_id": "2046082", "ec_numbers": [ "1.14.19.3" ], "accessions": [ "O95864" ] } ], "citations": [ { "id": "PMID:12713571", "title": "Identification of the delta-6 desaturase of human sebaceous glands: expression and enzyme activity", "journal": "J Invest Dermatol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12713571/" }, { "id": "PMID:12562861", "title": "Regulation of human delta-6 desaturase gene transcription: identification of a functional direct repeat-1 element", "journal": "J Lipid Res", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12562861/" }, { "id": "PMID:1346092", "title": "Comparison of the conversion rates of alpha-linolenic acid (18:3(n - 3)) and stearidonic acid (18:4(n - 3)) to longer polyunsaturated fatty acids in rats", "journal": "Biochim Biophys Acta", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1346092/" }, { "id": "PMID:11988075", "title": "The same rat Delta6-desaturase not only acts on 18- but also on 24-carbon fatty acids in very-long-chain polyunsaturated fatty acid biosynthesis", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11988075/" }, { "id": "PMID:19056654", "title": "Dietary stearidonic acid is a long chain (n-3) polyunsaturated fatty acid with potential health benefits", "journal": "J Nutr", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19056654/" } ], "x": 590, "y": 1700.4736842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046085", "label": "Activation of alpha-linolenic acid to alpha-linolenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:44936" ], "source_url": "https://reactome.org/content/detail/R-HSA-2046085", "summary": "Activation of alpha-linolenic acid to alpha-linolenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects ALA, ATP, CoA-SH to ALA-CoA, PPi, adenosine 5'-monophosphate in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:44936.", "equation": "ALA + ATP + CoA-SH → ALA-CoA + PPi + adenosine 5'-monophosphate", "inputs": [ { "node_id": "R-ALL-2046056", "reactome_id": "R-ALL-2046056", "label": "ALA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27432", "formula": "C18H30O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-211579", "reactome_id": "R-ALL-211579", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046062", "reactome_id": "R-ALL-2046062", "label": "ALA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:51985", "formula": "C39H64N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046049", "reactome_id": "R-ALL-2046049", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046064", "reactome_id": "R-ALL-2046064", "label": "adenosine 5'-monophosphate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of ACSL1 [endoplasmic reticulum membrane]", "activity_id": "2046080", "ec_numbers": [ "6.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:7061494", "title": "Discovery of an arachidonoyl coenzyme A synthetase in human platelets", "journal": "J Biol Chem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7061494/" } ], "x": 868.6666666666667, "y": 1661.2456140350878, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046087", "label": "Translocation of tetracosahexaenoyl-CoA to peroxisomes", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane · peroxisomal membrane · peroxisomal matrix", "compartment_ids": [ "GO:0005788", "GO:0005789", "GO:0005778", "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046087", "summary": "Translocation of tetracosahexaenoyl-CoA to peroxisomes is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATP, THA-CoA to ADP, PI, THA-CoA in endoplasmic reticulum lumen · endoplasmic reticulum membrane · peroxisomal membrane · peroxisomal matrix.", "equation": "ATP + THA-CoA → ADP + PI + THA-CoA", "inputs": [ { "node_id": "R-ALL-211579", "reactome_id": "R-ALL-211579", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2046054", "reactome_id": "R-ALL-2046054", "label": "THA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63540", "formula": "C45H70N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-211606", "reactome_id": "R-ALL-211606", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046047", "reactome_id": "R-ALL-2046047", "label": "THA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63540", "formula": "C45H70N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid transmembrane transporter activity of ABCD1 [peroxisomal membrane]", "activity_id": "2046078", "ec_numbers": [ "7.6.2.4" ], "accessions": [ "P33897" ] } ], "citations": [ { "id": "PMID:8656081", "title": "Docosahexaenoic acid synthesis in human skin fibroblasts involves peroxisomal retroconversion of tetracosahexaenoic acid", "journal": "J Lipid Res", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8656081/" }, { "id": "PMID:11500517", "title": "Peroxisomal straight-chain Acyl-CoA oxidase and D-bifunctional protein are essential for the retroconversion step in docosahexaenoic acid synthesis", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11500517/" }, { "id": "PMID:12897190", "title": "Studies on the metabolic fate of n-3 polyunsaturated fatty acids", "journal": "J Lipid Res", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12897190/" } ], "x": 868.6666666666667, "y": 1706.5087719298244, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-2046088", "label": "Elongation of stearidonoyl-CoA to eicosatetraenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046088", "summary": "Elongation of stearidonoyl-CoA to eicosatetraenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects SDA-CoA, Malonyl-CoA, NADPH to ETA-CoA, H2O, CO2 in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "SDA-CoA + Malonyl-CoA + 2 NADPH → ETA-CoA + H2O + CO2 + 2 NADP+ + CoA-SH", "inputs": [ { "node_id": "R-ALL-2046052", "reactome_id": "R-ALL-2046052", "label": "SDA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63545", "formula": "C39H62N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046066", "reactome_id": "R-ALL-2046066", "label": "Malonyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046051", "reactome_id": "R-ALL-2046051", "label": "ETA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63542", "formula": "C41H66N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159751", "reactome_id": "R-ALL-159751", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL1,2,3,5 [endoplasmic reticulum membrane]", "activity_id": "548806", "ec_numbers": [ "2.3.1.199" ], "accessions": [] } ], "citations": [ { "id": "PMID:10899997", "title": "Identification and characterization of an enzyme involved in the elongation of n-6 and n-3 polyunsaturated fatty acids", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10899997/" }, { "id": "PMID:12716664", "title": "Metabolism of stearidonic acid in human subjects: comparison with the metabolism of other n-3 fatty acids", "journal": "Am J Clin Nutr", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12716664/" } ], "x": 590, "y": 1694.438596491228, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046089", "label": "Desaturation of eicosatetraenoyl-CoA to eicosapentaenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046089", "summary": "Desaturation of eicosatetraenoyl-CoA to eicosapentaenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects ETA-CoA, O2, NADH to EPA-CoA, H2O, NAD+ in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "ETA-CoA + O2 + 2 NADH → EPA-CoA + 2 H2O + 2 NAD+", "inputs": [ { "node_id": "R-ALL-2046051", "reactome_id": "R-ALL-2046051", "label": "ETA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63542", "formula": "C41H66N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194697", "reactome_id": "R-ALL-194697", "label": "NADH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046059", "reactome_id": "R-ALL-2046059", "label": "EPA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63539", "formula": "C41H64N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-194653", "reactome_id": "R-ALL-194653", "label": "NAD+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "omega-6 fatty acid desaturase activity of FADS1 [endoplasmic reticulum membrane]", "activity_id": "2046077", "ec_numbers": null, "accessions": [ "O60427" ] } ], "citations": [ { "id": "PMID:10601301", "title": "Cloning, expression, and fatty acid regulation of the human delta-5 desaturase", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10601301/" }, { "id": "PMID:8096621", "title": "Comparison of the metabolism of alpha-linolenic acid and its delta 6 desaturation product, stearidonic acid, in cultured NIH-3T3 cells", "journal": "Lipids", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8096621/" } ], "x": 868.6666666666667, "y": 1700.4736842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046090", "label": "Elongation of docosapentaenoyl-CoA to tetracosapentaenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046090", "summary": "Elongation of docosapentaenoyl-CoA to tetracosapentaenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects DPA-CoA, Malonyl-CoA, NADPH to TPA-CoA, CO2, CoA-SH in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "DPA-CoA + Malonyl-CoA + 2 NADPH → TPA-CoA + CO2 + CoA-SH + H2O + 2 NADP+", "inputs": [ { "node_id": "R-ALL-2046057", "reactome_id": "R-ALL-2046057", "label": "DPA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63541", "formula": "C43H68N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046066", "reactome_id": "R-ALL-2046066", "label": "Malonyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046065", "reactome_id": "R-ALL-2046065", "label": "TPA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63543", "formula": "C45H72N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159751", "reactome_id": "R-ALL-159751", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL2 [endoplasmic reticulum membrane]", "activity_id": "548822", "ec_numbers": [ "2.3.1.199" ], "accessions": [ "Q9NXB9" ] } ], "citations": [ { "id": "PMID:14636670", "title": "Elongation of long-chain fatty acids", "journal": "Prog Lipid Res", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14636670/" }, { "id": "PMID:1834642", "title": "The metabolism of 7,10,13,16,19-docosapentaenoic acid to 4,7,10,13,16,19-docosahexaenoic acid in rat liver is independent of a 4-desaturase", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1834642/" } ], "x": 868.6666666666667, "y": 1694.438596491228, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046092", "label": "Desaturation of dihomo-gamma-lenolenoyl-CoA to arachidonoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046092", "summary": "Desaturation of dihomo-gamma-lenolenoyl-CoA to arachidonoyl-CoA is a Reactome release 97 curated reaction event. The source record connects DGL-CoA, O2, NADH to AA-CoA, H2O, NAD+ in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "DGL-CoA + O2 + 2 NADH → AA-CoA + 2 H2O + 2 NAD+", "inputs": [ { "node_id": "R-ALL-2046045", "reactome_id": "R-ALL-2046045", "label": "DGL-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27979", "formula": "C41H68N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194697", "reactome_id": "R-ALL-194697", "label": "NADH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046053", "reactome_id": "R-ALL-2046053", "label": "AA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57368", "formula": "C41H62N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-194653", "reactome_id": "R-ALL-194653", "label": "NAD+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "omega-6 fatty acid desaturase activity of FADS1 [endoplasmic reticulum membrane]", "activity_id": "2046077", "ec_numbers": null, "accessions": [ "O60427" ] } ], "citations": [ { "id": "PMID:10601301", "title": "Cloning, expression, and fatty acid regulation of the human delta-5 desaturase", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10601301/" }, { "id": "PMID:10769175", "title": "cDNA cloning and characterization of human Delta5-desaturase involved in the biosynthesis of arachidonic acid", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10769175/" } ], "x": 311.33333333333337, "y": 1703.4912280701753, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046093", "label": "Translocation of tetracosapentaenoyl-CoA to peroxisomes", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen · peroxisomal membrane · peroxisomal matrix", "compartment_ids": [ "GO:0005789", "GO:0005788", "GO:0005778", "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046093", "summary": "Translocation of tetracosapentaenoyl-CoA to peroxisomes is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATP, TPA-CoA to ADP, TPA-CoA, Pi in endoplasmic reticulum membrane · endoplasmic reticulum lumen · peroxisomal membrane · peroxisomal matrix.", "equation": "ATP + TPA-CoA → ADP + TPA-CoA + Pi", "inputs": [ { "node_id": "R-ALL-211579", "reactome_id": "R-ALL-211579", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2046050", "reactome_id": "R-ALL-2046050", "label": "TPA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:74084", "formula": "C45H68N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-211606", "reactome_id": "R-ALL-211606", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2046055", "reactome_id": "R-ALL-2046055", "label": "TPA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:74084", "formula": "C45H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5228339", "reactome_id": "R-ALL-5228339", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid transmembrane transporter activity of ABCD1 [peroxisomal membrane]", "activity_id": "2046078", "ec_numbers": [ "7.6.2.4" ], "accessions": [ "P33897" ] } ], "citations": [ { "id": "PMID:9684854", "title": "Analysis of the putative role of 24-carbon polyunsaturated fatty acids in the biosynthesis of docosapentaenoic (22:5n-6) and docosahexaenoic (22:6n-3) acids", "journal": "FEBS Lett", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9684854/" }, { "id": "PMID:11500517", "title": "Peroxisomal straight-chain Acyl-CoA oxidase and D-bifunctional protein are essential for the retroconversion step in docosahexaenoic acid synthesis", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11500517/" }, { "id": "PMID:12897190", "title": "Studies on the metabolic fate of n-3 polyunsaturated fatty acids", "journal": "J Lipid Res", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12897190/" } ], "x": 311.33333333333337, "y": 1709.5263157894738, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-2046094", "label": "Elongation of gamma-lenolenoyl-CoA to dihomo-gamma-lenolenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046094", "summary": "Elongation of gamma-lenolenoyl-CoA to dihomo-gamma-lenolenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects GLA-CoA, Malonyl-CoA, NADPH to DGL-CoA, H2O, CO2 in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "GLA-CoA + Malonyl-CoA + 2 NADPH → DGL-CoA + H2O + CO2 + 2 NADP+ + CoA-SH", "inputs": [ { "node_id": "R-ALL-2046042", "reactome_id": "R-ALL-2046042", "label": "GLA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15508", "formula": "C39H64N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046066", "reactome_id": "R-ALL-2046066", "label": "Malonyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046045", "reactome_id": "R-ALL-2046045", "label": "DGL-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27979", "formula": "C41H68N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159751", "reactome_id": "R-ALL-159751", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL1,2,3,5 [endoplasmic reticulum membrane]", "activity_id": "548806", "ec_numbers": [ "2.3.1.199" ], "accessions": [] } ], "citations": [ { "id": "PMID:8609415", "title": "Metabolism of gammalinolenic acid in human neutrophils", "journal": "J Immunol", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8609415/" }, { "id": "PMID:9732298", "title": "Importance of dietary gamma-linolenic acid in human health and nutrition", "journal": "J Nutr", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9732298/" }, { "id": "PMID:10970790", "title": "Cloning of a human cDNA encoding a novel enzyme involved in the elongation of long-chain polyunsaturated fatty acids", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10970790/" } ], "x": 311.33333333333337, "y": 1697.4561403508771, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046095", "label": "Elongation of docosatetraenoyl-CoA to tetracosatetraenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046095", "summary": "Elongation of docosatetraenoyl-CoA to tetracosatetraenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects DTA-CoA, Malonyl-CoA, NADPH to TTA-CoA, CO2, CoA-SH in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "DTA-CoA + Malonyl-CoA + 2 NADPH → TTA-CoA + CO2 + CoA-SH + H2O", "inputs": [ { "node_id": "R-ALL-2046060", "reactome_id": "R-ALL-2046060", "label": "DTA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63544", "formula": "C43H70N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046066", "reactome_id": "R-ALL-2046066", "label": "Malonyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046048", "reactome_id": "R-ALL-2046048", "label": "TTA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63548", "formula": "C45H74N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159751", "reactome_id": "R-ALL-159751", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL2 [endoplasmic reticulum membrane]", "activity_id": "548822", "ec_numbers": [ "2.3.1.199" ], "accessions": [ "Q9NXB9" ] } ], "citations": [ { "id": "PMID:5411545", "title": "The biosynthesis of delta-9,12,15,18-tetracosatetraenoic and of delta-6,9,12,15,18-tetracosapentaenoic acids by rat testes", "journal": "J Biol Chem", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/5411545/" }, { "id": "PMID:14636670", "title": "Elongation of long-chain fatty acids", "journal": "Prog Lipid Res", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14636670/" }, { "id": "PMID:9704073", "title": "Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles", "journal": "J Lipid Res", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/9704073/" } ], "x": 311.33333333333337, "y": 1700.4736842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046096", "label": "Desaturation of Linoleoyl-CoA to gamma-linolenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046096", "summary": "Desaturation of Linoleoyl-CoA to gamma-linolenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects LA-CoA, O2, NADH to GLA-CoA, H2O, NAD+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "LA-CoA + O2 + 2 NADH → GLA-CoA + 2 H2O + 2 NAD+", "inputs": [ { "node_id": "R-ALL-2046063", "reactome_id": "R-ALL-2046063", "label": "LA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57383", "formula": "C39H62N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194697", "reactome_id": "R-ALL-194697", "label": "NADH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046042", "reactome_id": "R-ALL-2046042", "label": "GLA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15508", "formula": "C39H64N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-194653", "reactome_id": "R-ALL-194653", "label": "NAD+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyl-CoA 6-desaturase activity of FADS2 [endoplasmic reticulum membrane]", "activity_id": "2046082", "ec_numbers": [ "1.14.19.3" ], "accessions": [ "O95864" ] } ], "citations": [ { "id": "PMID:12713571", "title": "Identification of the delta-6 desaturase of human sebaceous glands: expression and enzyme activity", "journal": "J Invest Dermatol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12713571/" }, { "id": "PMID:12562861", "title": "Regulation of human delta-6 desaturase gene transcription: identification of a functional direct repeat-1 element", "journal": "J Lipid Res", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12562861/" }, { "id": "PMID:11988075", "title": "The same rat Delta6-desaturase not only acts on 18- but also on 24-carbon fatty acids in very-long-chain polyunsaturated fatty acid biosynthesis", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11988075/" } ], "x": 590, "y": 1703.4912280701753, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046097", "label": "Desaturation of tetracosatetraenoyl-CoA to tetracosapentaenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046097", "summary": "Desaturation of tetracosatetraenoyl-CoA to tetracosapentaenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects TTA-CoA, O2, NADH to TPA-CoA, H2O, NAD+ in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "TTA-CoA + O2 + 2 NADH → TPA-CoA + 2 H2O + 2 NAD+", "inputs": [ { "node_id": "R-ALL-2046048", "reactome_id": "R-ALL-2046048", "label": "TTA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63548", "formula": "C45H74N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194697", "reactome_id": "R-ALL-194697", "label": "NADH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046050", "reactome_id": "R-ALL-2046050", "label": "TPA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:74084", "formula": "C45H68N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-194653", "reactome_id": "R-ALL-194653", "label": "NAD+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyl-CoA 6-desaturase activity of FADS2 [endoplasmic reticulum membrane]", "activity_id": "2046082", "ec_numbers": [ "1.14.19.3" ], "accessions": [ "O95864" ] } ], "citations": [ { "id": "PMID:1834642", "title": "The metabolism of 7,10,13,16,19-docosapentaenoic acid to 4,7,10,13,16,19-docosahexaenoic acid in rat liver is independent of a 4-desaturase", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1834642/" }, { "id": "PMID:11988075", "title": "The same rat Delta6-desaturase not only acts on 18- but also on 24-carbon fatty acids in very-long-chain polyunsaturated fatty acid biosynthesis", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11988075/" } ], "x": 868.6666666666667, "y": 1703.4912280701753, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046098", "label": "Activation of linoleic acid to linoleoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:33651" ], "source_url": "https://reactome.org/content/detail/R-HSA-2046098", "summary": "Activation of linoleic acid to linoleoyl-CoA is a Reactome release 97 curated reaction event. The source record connects LINA, ATP, CoA-SH to LA-CoA, PPi, adenosine 5'-monophosphate in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:33651.", "equation": "LINA + ATP + CoA-SH → LA-CoA + PPi + adenosine 5'-monophosphate", "inputs": [ { "node_id": "R-ALL-2046044", "reactome_id": "R-ALL-2046044", "label": "LINA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17351", "formula": "C18H32O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-211579", "reactome_id": "R-ALL-211579", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046063", "reactome_id": "R-ALL-2046063", "label": "LA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57383", "formula": "C39H62N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046049", "reactome_id": "R-ALL-2046049", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046064", "reactome_id": "R-ALL-2046064", "label": "adenosine 5'-monophosphate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of ACSL1 [endoplasmic reticulum membrane]", "activity_id": "2046080", "ec_numbers": [ "6.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:7061494", "title": "Discovery of an arachidonoyl coenzyme A synthetase in human platelets", "journal": "J Biol Chem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7061494/" } ], "x": 311.33333333333337, "y": 1664.2631578947369, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046099", "label": "Desaturation of tetracosapentaenoyl-CoA to tetracosahexaenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046099", "summary": "Desaturation of tetracosapentaenoyl-CoA to tetracosahexaenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects TPA-CoA, O2, NADH to THA-CoA, H2O, NAD+ in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "TPA-CoA + O2 + 2 NADH → THA-CoA + 2 H2O + 2 NAD+", "inputs": [ { "node_id": "R-ALL-2046065", "reactome_id": "R-ALL-2046065", "label": "TPA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63543", "formula": "C45H72N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194697", "reactome_id": "R-ALL-194697", "label": "NADH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046054", "reactome_id": "R-ALL-2046054", "label": "THA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63540", "formula": "C45H70N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-194653", "reactome_id": "R-ALL-194653", "label": "NAD+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acyl-CoA 6-desaturase activity of FADS2 [endoplasmic reticulum membrane]", "activity_id": "2046082", "ec_numbers": [ "1.14.19.3" ], "accessions": [ "O95864" ] } ], "citations": [], "x": 311.33333333333337, "y": 1706.5087719298244, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046100", "label": "Elongation of eicosapentaenoyl-CoA to docosapentaenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046100", "summary": "Elongation of eicosapentaenoyl-CoA to docosapentaenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects EPA-CoA, Malonyl-CoA, NADPH to DPA-CoA, CO2, CoA-SH in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "EPA-CoA + Malonyl-CoA + 2 NADPH → DPA-CoA + CO2 + CoA-SH + H2O + 2 NADP+", "inputs": [ { "node_id": "R-ALL-2046059", "reactome_id": "R-ALL-2046059", "label": "EPA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63539", "formula": "C41H64N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2046066", "reactome_id": "R-ALL-2046066", "label": "Malonyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2046057", "reactome_id": "R-ALL-2046057", "label": "DPA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63541", "formula": "C43H68N7O17P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159751", "reactome_id": "R-ALL-159751", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL5/ELOVL2 [endoplasmic reticulum membrane]", "activity_id": "2046081", "ec_numbers": [ "2.3.1.199" ], "accessions": [] } ], "citations": [ { "id": "PMID:12323085", "title": "Eicosapentaenoic and docosapentaenoic acids are the principal products of alpha-linolenic acid metabolism in young men*", "journal": "Br J Nutr", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12323085/" }, { "id": "PMID:12323090", "title": "Conversion of alpha-linolenic acid to eicosapentaenoic, docosapentaenoic and docosahexaenoic acids in young women", "journal": "Br J Nutr", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12323090/" }, { "id": "PMID:8832760", "title": "Docosapentaenoic acid (22:5, n-3), an elongation metabolite of eicosapentaenoic acid (20:5, n-3), is a potent stimulator of endothelial cell migration on pretreatment in vitro", "journal": "Prostaglandins Leukot Essent Fatty Acids", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8832760/" } ], "x": 590, "y": 1697.4561403508771, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046101", "label": "Peroxisomal beta-oxidation of tetracosapentaenoyl-CoA to Docosapentaenoyl-CoA", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046101", "summary": "Peroxisomal beta-oxidation of tetracosapentaenoyl-CoA to Docosapentaenoyl-CoA is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects TPA-CoA to DPA in peroxisomal matrix.", "equation": "TPA-CoA → DPA", "inputs": [ { "node_id": "R-ALL-2046055", "reactome_id": "R-ALL-2046055", "label": "TPA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:74084", "formula": "C45H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2066764", "reactome_id": "R-ALL-2066764", "label": "DPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77226", "formula": "C22H33O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9684854", "title": "Analysis of the putative role of 24-carbon polyunsaturated fatty acids in the biosynthesis of docosapentaenoic (22:5n-6) and docosahexaenoic (22:6n-3) acids", "journal": "FEBS Lett", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9684854/" } ], "x": 590, "y": 1754.7894736842104, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-2046175", "label": "CHST1 transfers sulfate to Gal on keratan chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046175", "summary": "CHST1 transfers sulfate to Gal on keratan chain is a Reactome release 97 curated reaction event. The source record connects KSPG(1), PAPS to KSPG(2), PAP in Golgi membrane · Golgi lumen.", "equation": "KSPG(1) + PAPS → KSPG(2) + PAP", "inputs": [ { "node_id": "R-HSA-2046166", "reactome_id": "R-HSA-2046166", "label": "KSPG(1)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2046288", "reactome_id": "R-HSA-2046288", "label": "KSPG(2)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "keratan sulfotransferase activity of CHST1 [Golgi membrane]", "activity_id": "2046178", "ec_numbers": [ "2.8.2.21" ], "accessions": [ "O43916" ] } ], "citations": [ { "id": "PMID:9405439", "title": "Molecular cloning and characterization of human keratan sulfate Gal-6-sulfotransferase", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9405439/" } ], "x": 2660, "y": 1030.8030303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046180", "label": "KSPG is secreted from the cell", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi lumen · extracellular region", "compartment_ids": [ "GO:0005796", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046180", "summary": "KSPG is secreted from the cell is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects KSPG(2) to KSPG(2) in Golgi lumen · extracellular region.", "equation": "KSPG(2) → KSPG(2)", "inputs": [ { "node_id": "R-HSA-2046288", "reactome_id": "R-HSA-2046288", "label": "KSPG(2)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2046244", "reactome_id": "R-HSA-2046244", "label": "KSPG(2)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11030741", "title": "Keratan sulfate: structure, biosynthesis, and function", "journal": "Glycobiology", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11030741/" } ], "x": 2381.3333333333335, "y": 1113.3181818181818, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2046222", "label": "CHST2,3,5,6 transfer sulfate to GlcNAc on keratan chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:67857" ], "source_url": "https://reactome.org/content/detail/R-HSA-2046222", "summary": "CHST2,3,5,6 transfer sulfate to GlcNAc on keratan chain is a Reactome release 97 curated reaction event. The source record connects Keratan(4)-PG, PAPS to PAP, KSPG(1), H+ in Golgi membrane · Golgi lumen and cross-references RHEA:67857.", "equation": "Keratan(4)-PG + PAPS → PAP + KSPG(1) + H+", "inputs": [ { "node_id": "R-HSA-2046203", "reactome_id": "R-HSA-2046203", "label": "Keratan(4)-PG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2046166", "reactome_id": "R-HSA-2046166", "label": "KSPG(1)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N-acetylglucosamine 6-O-sulfotransferase activity of CHST2,3,5,6 [Golgi membrane]", "activity_id": "2046153", "ec_numbers": [ "2.8.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11042394", "title": "Functional expression and genomic structure of human N-acetylglucosamine-6-O-sulfotransferase that transfers sulfate to beta-N-acetylglucosamine at the nonreducing end of an N-acetyllactosamine sequence", "journal": "Biochim Biophys Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11042394/" }, { "id": "PMID:10491328", "title": "Cloning and characterization of a mammalian N-acetylglucosamine-6-sulfotransferase that is highly restricted to intestinal tissue", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10491328/" }, { "id": "PMID:12218059", "title": "Enzymatic synthesis in vitro of the disulfated disaccharide unit of corneal keratan sulfate", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12218059/" } ], "x": 2381.3333333333335, "y": 853.9848484848485, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046239", "label": "Extracellular KSPG translocates to the lysosome for degradation", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "extracellular region · lysosomal lumen", "compartment_ids": [ "GO:0005576", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2046239", "summary": "Extracellular KSPG translocates to the lysosome for degradation is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects KSPG(2) to KSPG(2) in extracellular region · lysosomal lumen.", "equation": "KSPG(2) → KSPG(2)", "inputs": [ { "node_id": "R-HSA-2046244", "reactome_id": "R-HSA-2046244", "label": "KSPG(2)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2046191", "reactome_id": "R-HSA-2046191", "label": "KSPG(2)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15647514", "title": "Lysosomal metabolism of glycoproteins", "journal": "Glycobiology", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15647514/" } ], "x": 2660, "y": 1113.3181818181818, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2046265", "label": "B4GALTs transfer Gal to the keratan chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:22933" ], "source_url": "https://reactome.org/content/detail/R-HSA-2046265", "summary": "B4GALTs transfer Gal to the keratan chain is a Reactome release 97 curated reaction event. The source record connects Keratan(1)-PG, UDP-Gal to UDP, Keratan(2)-PG, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:22933.", "equation": "Keratan(1)-PG + UDP-Gal → UDP + Keratan(2)-PG + H+", "inputs": [ { "node_id": "R-HSA-2046187", "reactome_id": "R-HSA-2046187", "label": "Keratan(1)-PG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-735682", "reactome_id": "R-ALL-735682", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2046206", "reactome_id": "R-HSA-2046206", "label": "Keratan(2)-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity of B4GALT1-6 homodimers [Golgi membrane]", "activity_id": "975894", "ec_numbers": [ "2.4.1.38" ], "accessions": [] } ], "citations": [ { "id": "PMID:11901181", "title": "Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId", "journal": "J Clin Invest", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11901181/" }, { "id": "PMID:1714903", "title": "Evidence for a molecular distinction between Golgi and cell surface forms of beta 1,4-galactosyltransferase", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1714903/" }, { "id": "PMID:11588157", "title": "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells", "journal": "Glycobiology", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11588157/" }, { "id": "PMID:9597550", "title": "The expanding beta 4-galactosyltransferase gene family: messages from the databanks", "journal": "Glycobiology", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9597550/" }, { "id": "PMID:17021253", "title": "Transition of galactosyltransferase 1 from trans-Golgi cisterna to the trans-Golgi network is signal mediated", "journal": "Mol Biol Cell", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17021253/" } ], "x": 2660, "y": 853.9848484848485, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046285", "label": "ST3GAL1-4,6 optionally cap keratan chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:52317" ], "source_url": "https://reactome.org/content/detail/R-HSA-2046285", "summary": "ST3GAL1-4,6 optionally cap keratan chain is a Reactome release 97 curated reaction event. The source record connects Keratan(3)-PG, CMP-Neu5Ac to CMP, Keratan(4)-PG, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:52317.", "equation": "Keratan(3)-PG + CMP-Neu5Ac → CMP + Keratan(4)-PG + H+", "inputs": [ { "node_id": "R-HSA-2046314", "reactome_id": "R-HSA-2046314", "label": "Keratan(3)-PG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-727815", "reactome_id": "R-ALL-727815", "label": "CMP-Neu5Ac", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57812", "formula": "C20H29N4O16P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-727810", "reactome_id": "R-ALL-727810", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2046203", "reactome_id": "R-HSA-2046203", "label": "Keratan(4)-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "beta-galactoside (CMP) alpha-2,3-sialyltransferase activity of ST3GAL1-4,6 [Golgi membrane]", "activity_id": "2046209", "ec_numbers": [ "2.4.3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:8611500", "title": "Characterization of two glycolipid: alpha 2-3sialyltransferases, SAT-3 (CMP-NeuAc:nLcOse4Cer alpha 2-3sialyltransferase) and SAT-4 (CMP-NeuAc:GgOse4Cer alpha 2-3sialyltransferase), from human colon carcinoma (Colo 205) cell line", "journal": "Biochemistry", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8611500/" }, { "id": "PMID:8333853", "title": "Cloning and expression of human Gal beta 1,3(4)GlcNAc alpha 2,3-sialyltransferase", "journal": "Biochem Biophys Res Commun", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8333853/" }, { "id": "PMID:10504389", "title": "Molecular cloning and expression of Galbeta1,3GalNAc alpha2, 3-sialyltransferase from human fetal liver", "journal": "Eur J Biochem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10504389/" }, { "id": "PMID:8920913", "title": "Molecular cloning and expression of human Gal beta 1,3GalNAc alpha 2,3-sialytransferase (hST3Gal II)", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8920913/" }, { "id": "PMID:10206952", "title": "Molecular cloning of a novel alpha2,3-sialyltransferase (ST3Gal VI) that sialylates type II lactosamine structures on glycoproteins and glycolipids", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10206952/" } ], "x": 2938.666666666667, "y": 853.9848484848485, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2046298", "label": "B4GALTs transfer Gal to a branch of keratan", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:22933" ], "source_url": "https://reactome.org/content/detail/R-HSA-2046298", "summary": "B4GALTs transfer Gal to a branch of keratan is a Reactome release 97 curated reaction event. The source record connects Keratan(2)-PG, UDP-Gal to UDP, Keratan(3)-PG, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:22933.", "equation": "Keratan(2)-PG + UDP-Gal → UDP + Keratan(3)-PG + H+", "inputs": [ { "node_id": "R-HSA-2046206", "reactome_id": "R-HSA-2046206", "label": "Keratan(2)-PG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-735682", "reactome_id": "R-ALL-735682", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2046314", "reactome_id": "R-HSA-2046314", "label": "Keratan(3)-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity of B4GALT1-6 homodimers [Golgi membrane]", "activity_id": "975894", "ec_numbers": [ "2.4.1.38" ], "accessions": [] } ], "citations": [ { "id": "PMID:11901181", "title": "Deficiency of UDP-galactose:N-acetylglucosamine beta-1,4-galactosyltransferase I causes the congenital disorder of glycosylation type IId", "journal": "J Clin Invest", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11901181/" }, { "id": "PMID:1714903", "title": "Evidence for a molecular distinction between Golgi and cell surface forms of beta 1,4-galactosyltransferase", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1714903/" }, { "id": "PMID:11588157", "title": "Galactosylation of N-linked oligosaccharides by human beta-1,4-galactosyltransferases I, II, III, IV, V, and VI expressed in Sf-9 cells", "journal": "Glycobiology", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11588157/" }, { "id": "PMID:9597550", "title": "The expanding beta 4-galactosyltransferase gene family: messages from the databanks", "journal": "Glycobiology", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9597550/" }, { "id": "PMID:17021253", "title": "Transition of galactosyltransferase 1 from trans-Golgi cisterna to the trans-Golgi network is signal mediated", "journal": "Mol Biol Cell", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17021253/" } ], "x": 2381.3333333333335, "y": 865.7727272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2065233", "label": "CS is cleaved from its proteoglycan", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2065233", "summary": "CS is cleaved from its proteoglycan is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects H2O, CSPGs to CS/DS core proteins, C4S/C6S chains in lysosomal lumen.", "equation": "H2O + CSPGs → CS/DS core proteins + C4S/C6S chains", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2065110", "reactome_id": "R-HSA-2065110", "label": "CSPGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2065248", "reactome_id": "R-HSA-2065248", "label": "CS/DS core proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-2065251", "reactome_id": "R-ALL-2065251", "label": "C4S/C6S chains", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "hydrolase activity, acting on glycosyl bonds of beta-xylosidase [lysosomal lumen]", "activity_id": "2065101", "ec_numbers": [ "3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:3134052", "title": "Demonstration of an endo-beta-galactosidase and an endo-beta-xylosidase that degrade the proteoglycan linkage region", "journal": "Biochim Biophys Acta", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3134052/" } ], "x": 2938.666666666667, "y": 1066.1666666666665, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-2065539", "label": "SREBP1A,1C,2:Importin beta-1 dissociates", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2065539", "summary": "SREBP1A,1C,2:Importin beta-1 dissociates is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C,2:Importin beta-1, RAN:GTP to SREBP1A,1C,2 dimer, Importin-beta:Ran GTP complex in nucleoplasm.", "equation": "SREBP1A,1C,2:Importin beta-1 + RAN:GTP → SREBP1A,1C,2 dimer + Importin-beta:Ran GTP complex", "inputs": [ { "node_id": "R-HSA-2065543", "reactome_id": "R-HSA-2065543", "label": "SREBP1A,1C,2:Importin beta-1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-180738", "reactome_id": "R-HSA-180738", "label": "RAN:GTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655744", "reactome_id": "R-HSA-1655744", "label": "SREBP1A,1C,2 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-180719", "reactome_id": "R-HSA-180719", "label": "Importin-beta:Ran GTP complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "importin-alpha family protein binding activity of RAN:GTP [nucleoplasm]", "activity_id": "9959904", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10397761", "title": "Nuclear import of sterol regulatory element-binding protein-2, a basic helix-loop-helix-leucine zipper (bHLH-Zip)-containing transcription factor, occurs through the direct interaction of importin beta with HLH-Zip", "journal": "Mol Biol Cell", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10397761/" } ], "x": 5008.666666666667, "y": 1759.9166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2065549", "label": "SREBP1A,1C,2 binds SREBP1A,1C,2 forming dimers", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2065549", "summary": "SREBP1A,1C,2 binds SREBP1A,1C,2 forming dimers is a Reactome release 97 curated reaction event. The source record connects SREBF1A,1C,2 cleaved by S2P to SREBP1A,1C,2 dimer in cytosol.", "equation": "2 SREBF1A,1C,2 cleaved by S2P → SREBP1A,1C,2 dimer", "inputs": [ { "node_id": "R-HSA-1655732", "reactome_id": "R-HSA-1655732", "label": "SREBF1A,1C,2 cleaved by S2P", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2065548", "reactome_id": "R-HSA-2065548", "label": "SREBP1A,1C,2 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11283257", "title": "Dimerization of sterol regulatory element-binding protein 2 via the helix-loop-helix-leucine zipper domain is a prerequisite for its nuclear localization mediated by importin beta", "journal": "Mol Cell Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11283257/" } ], "x": 5008.666666666667, "y": 1824.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2065550", "label": "SREBP1A,1C,2 binds Importin beta-1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2065550", "summary": "SREBP1A,1C,2 binds Importin beta-1 is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C,2 dimer, KPNB1 to SREBP1A,1C,2:Importin beta-1 in cytosol.", "equation": "SREBP1A,1C,2 dimer + KPNB1 → SREBP1A,1C,2:Importin beta-1", "inputs": [ { "node_id": "R-HSA-2065548", "reactome_id": "R-HSA-2065548", "label": "SREBP1A,1C,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-180733", "reactome_id": "R-HSA-180733", "label": "KPNB1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2065556", "reactome_id": "R-HSA-2065556", "label": "SREBP1A,1C,2:Importin beta-1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "nuclear import signal receptor activity of KPNB1 [cytosol]", "activity_id": "9959902", "ec_numbers": null, "accessions": [ "Q14974" ] } ], "citations": [ { "id": "PMID:10397761", "title": "Nuclear import of sterol regulatory element-binding protein-2, a basic helix-loop-helix-leucine zipper (bHLH-Zip)-containing transcription factor, occurs through the direct interaction of importin beta with HLH-Zip", "journal": "Mol Biol Cell", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10397761/" }, { "id": "PMID:31875875", "title": "Mutant huntingtin interacts with the sterol regulatory element-binding proteins and impairs their nuclear import", "journal": "Hum Mol Genet", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/31875875/" } ], "x": 4451.333333333333, "y": 1765.810606060606, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2065966", "label": "SREBP1A,1C,2 binds the TM7SF2 promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2065966", "summary": "SREBP1A,1C,2 binds the TM7SF2 promoter is a Reactome release 97 curated reaction event. The source record connects NF-Y, TM7SF2 gene, SREBP1A,1C,2 dimer to SREBP1A,1C,2:NF-Y:TM7SF2 gene in nucleoplasm.", "equation": "NF-Y + TM7SF2 gene + SREBP1A,1C,2 dimer → SREBP1A,1C,2:NF-Y:TM7SF2 gene", "inputs": [ { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393984", "reactome_id": "R-HSA-2393984", "label": "TM7SF2 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-1655744", "reactome_id": "R-HSA-1655744", "label": "SREBP1A,1C,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655714", "reactome_id": "R-HSA-1655714", "label": "SREBP1A,1C,2:NF-Y:TM7SF2 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of NF-Y,SREBP1A,1C,2 [nucleoplasm]", "activity_id": "9987240", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:20138239", "title": "Activation of TM7SF2 promoter by SREBP-2 depends on a new sterol regulatory element, a GC-box, and an inverted CCAAT-box", "journal": "Biochim Biophys Acta", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20138239/" }, { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" } ], "x": 4730, "y": 1765.810606060606, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2066778", "label": "Hydration of delta2-tetracosaheptaenoyl-CoA to 3-hydroxy tetracosahexaenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2066778", "summary": "Hydration of delta2-tetracosaheptaenoyl-CoA to 3-hydroxy tetracosahexaenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects delta2-THA-CoA, H2O to 1-3-hydroxy-THA-CoA in peroxisomal matrix.", "equation": "delta2-THA-CoA + H2O → 1-3-hydroxy-THA-CoA", "inputs": [ { "node_id": "R-ALL-2066767", "reactome_id": "R-ALL-2066767", "label": "delta2-THA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65139", "formula": "C45H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2066766", "reactome_id": "R-ALL-2066766", "label": "1-3-hydroxy-THA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65130", "formula": "C45H70N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-hydroxyacyl-CoA dehydratase activity of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "389989", "ec_numbers": [ "4.2.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:8656081", "title": "Docosahexaenoic acid synthesis in human skin fibroblasts involves peroxisomal retroconversion of tetracosahexaenoic acid", "journal": "J Lipid Res", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8656081/" }, { "id": "PMID:11734571", "title": "Identification of the peroxisomal beta-oxidation enzymes involved in the biosynthesis of docosahexaenoic acid", "journal": "J Lipid Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11734571/" }, { "id": "PMID:11500517", "title": "Peroxisomal straight-chain Acyl-CoA oxidase and D-bifunctional protein are essential for the retroconversion step in docosahexaenoic acid synthesis", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11500517/" } ], "x": 590, "y": 1718.578947368421, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2066779", "label": "Conversion of DHA-CoA to docosahexaenoic acid (DHA)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:67701" ], "source_url": "https://reactome.org/content/detail/R-HSA-2066779", "summary": "Conversion of DHA-CoA to docosahexaenoic acid (DHA) is a Reactome release 97 curated reaction event. The source record connects DHA-CoA, H2O to DHA, CoA-SH in peroxisomal matrix and cross-references RHEA:67701.", "equation": "DHA-CoA + H2O → DHA + CoA-SH", "inputs": [ { "node_id": "R-ALL-2066765", "reactome_id": "R-ALL-2066765", "label": "DHA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65132", "formula": "C43H66N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2066763", "reactome_id": "R-ALL-2066763", "label": "DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acetyl-CoA hydrolase activity of ACOT8 [peroxisomal matrix]", "activity_id": "390301", "ec_numbers": [ "3.1.2.1" ], "accessions": [ "O14734" ] } ], "citations": [ { "id": "PMID:18538142", "title": "Novel functions of acyl-CoA thioesterases and acyltransferases as auxiliary enzymes in peroxisomal lipid metabolism", "journal": "Prog Lipid Res", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18538142/" } ], "x": 590, "y": 1676.3333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2066780", "label": "Dehydrogenation of 3-hydroxy tetracosahexaenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2066780", "summary": "Dehydrogenation of 3-hydroxy tetracosahexaenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 1-3-hydroxy-THA-CoA to 1-3-oxo-THA-CoA in peroxisomal matrix.", "equation": "1-3-hydroxy-THA-CoA → 1-3-oxo-THA-CoA", "inputs": [ { "node_id": "R-ALL-2066766", "reactome_id": "R-ALL-2066766", "label": "1-3-hydroxy-THA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65130", "formula": "C45H70N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2066762", "reactome_id": "R-ALL-2066762", "label": "1-3-oxo-THA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65131", "formula": "C45H68N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-hydroxyacyl-CoA dehydratase activity of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "389989", "ec_numbers": [ "4.2.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:8656081", "title": "Docosahexaenoic acid synthesis in human skin fibroblasts involves peroxisomal retroconversion of tetracosahexaenoic acid", "journal": "J Lipid Res", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8656081/" }, { "id": "PMID:11734571", "title": "Identification of the peroxisomal beta-oxidation enzymes involved in the biosynthesis of docosahexaenoic acid", "journal": "J Lipid Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11734571/" } ], "x": 311.33333333333337, "y": 1730.6491228070174, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2066781", "label": "Formation of DHA-CoA catalysed by sterol carrier protein X (SCPx)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2066781", "summary": "Formation of DHA-CoA catalysed by sterol carrier protein X (SCPx) is a Reactome release 97 curated reaction event. The source record connects 1-3-oxo-THA-CoA, CoA-SH to DHA-CoA, propionyl CoA in peroxisomal matrix.", "equation": "1-3-oxo-THA-CoA + CoA-SH → DHA-CoA + propionyl CoA", "inputs": [ { "node_id": "R-ALL-2066762", "reactome_id": "R-ALL-2066762", "label": "1-3-oxo-THA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65131", "formula": "C45H68N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2066765", "reactome_id": "R-ALL-2066765", "label": "DHA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65132", "formula": "C43H66N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192314", "reactome_id": "R-ALL-192314", "label": "propionyl CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57392", "formula": "C24H36N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "propionyl-CoA C2-trimethyltridecanoyltransferase activity of SCP2 [peroxisomal matrix]", "activity_id": "2066783", "ec_numbers": [ "2.3.1.176" ], "accessions": [ "P22307" ] } ], "citations": [ { "id": "PMID:8656081", "title": "Docosahexaenoic acid synthesis in human skin fibroblasts involves peroxisomal retroconversion of tetracosahexaenoic acid", "journal": "J Lipid Res", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8656081/" }, { "id": "PMID:11734571", "title": "Identification of the peroxisomal beta-oxidation enzymes involved in the biosynthesis of docosahexaenoic acid", "journal": "J Lipid Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11734571/" } ], "x": 590, "y": 1712.5438596491229, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2066782", "label": "Translocation of DPA to the ER", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "peroxisomal matrix · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005782", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2066782", "summary": "Translocation of DPA to the ER is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DPA to DPA in peroxisomal matrix · endoplasmic reticulum lumen.", "equation": "DPA → DPA", "inputs": [ { "node_id": "R-ALL-2066764", "reactome_id": "R-ALL-2066764", "label": "DPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77226", "formula": "C22H33O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2066768", "reactome_id": "R-ALL-2066768", "label": "DPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77226", "formula": "C22H33O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20480548", "title": "Acyl-coenzyme A synthetases in metabolic control", "journal": "Curr Opin Lipidol", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20480548/" }, { "id": "PMID:11500517", "title": "Peroxisomal straight-chain Acyl-CoA oxidase and D-bifunctional protein are essential for the retroconversion step in docosahexaenoic acid synthesis", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11500517/" } ], "x": 868.6666666666667, "y": 1754.7894736842104, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2066785", "label": "Retranslocation of DHA back to ER", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · peroxisomal matrix", "compartment_ids": [ "GO:0005788", "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2066785", "summary": "Retranslocation of DHA back to ER is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DHA to DHA in endoplasmic reticulum lumen · peroxisomal matrix.", "equation": "DHA → DHA", "inputs": [ { "node_id": "R-ALL-2066763", "reactome_id": "R-ALL-2066763", "label": "DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2066770", "reactome_id": "R-ALL-2066770", "label": "DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:18538142", "title": "Novel functions of acyl-CoA thioesterases and acyltransferases as auxiliary enzymes in peroxisomal lipid metabolism", "journal": "Prog Lipid Res", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18538142/" } ], "x": 311.33333333333337, "y": 1757.8070175438597, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2066787", "label": "Oxidation of tetracosapentaenoyl-CoA to delta2-tetracosaheptaenoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:39120" ], "source_url": "https://reactome.org/content/detail/R-HSA-2066787", "summary": "Oxidation of tetracosapentaenoyl-CoA to delta2-tetracosaheptaenoyl-CoA is a Reactome release 97 curated reaction event. The source record connects THA-CoA, O2 to delta2-THA-CoA, H2O2 in peroxisomal matrix and cross-references RHEA:39120.", "equation": "THA-CoA + O2 → delta2-THA-CoA + H2O2", "inputs": [ { "node_id": "R-ALL-2046047", "reactome_id": "R-ALL-2046047", "label": "THA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63540", "formula": "C45H70N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2066767", "reactome_id": "R-ALL-2066767", "label": "delta2-THA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65139", "formula": "C45H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA oxidase activity of ACOX1 dimer [peroxisomal matrix]", "activity_id": "390231", "ec_numbers": [ "1.3.3.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:8656081", "title": "Docosahexaenoic acid synthesis in human skin fibroblasts involves peroxisomal retroconversion of tetracosahexaenoic acid", "journal": "J Lipid Res", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8656081/" }, { "id": "PMID:11734571", "title": "Identification of the peroxisomal beta-oxidation enzymes involved in the biosynthesis of docosahexaenoic acid", "journal": "J Lipid Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11734571/" }, { "id": "PMID:11500517", "title": "Peroxisomal straight-chain Acyl-CoA oxidase and D-bifunctional protein are essential for the retroconversion step in docosahexaenoic acid synthesis", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11500517/" } ], "x": 868.6666666666667, "y": 1676.3333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2066788", "label": "Formation of DHA-CoA catalysed by 3-ketoacyl-CoA thiolase", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:39132" ], "source_url": "https://reactome.org/content/detail/R-HSA-2066788", "summary": "Formation of DHA-CoA catalysed by 3-ketoacyl-CoA thiolase is a Reactome release 97 curated reaction event. The source record connects 1-3-oxo-THA-CoA, CoA-SH to DHA-CoA, Ac-CoA in peroxisomal matrix and cross-references RHEA:39132.", "equation": "1-3-oxo-THA-CoA + CoA-SH → DHA-CoA + Ac-CoA", "inputs": [ { "node_id": "R-ALL-2066762", "reactome_id": "R-ALL-2066762", "label": "1-3-oxo-THA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65131", "formula": "C45H68N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2066765", "reactome_id": "R-ALL-2066765", "label": "DHA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65132", "formula": "C43H66N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-353123", "reactome_id": "R-ALL-353123", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetate CoA-transferase activity of ACAA1 [peroxisomal matrix]", "activity_id": "2066786", "ec_numbers": [ "2.8.3.8" ], "accessions": [ "P09110" ] } ], "citations": [ { "id": "PMID:8656081", "title": "Docosahexaenoic acid synthesis in human skin fibroblasts involves peroxisomal retroconversion of tetracosahexaenoic acid", "journal": "J Lipid Res", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8656081/" }, { "id": "PMID:11734571", "title": "Identification of the peroxisomal beta-oxidation enzymes involved in the biosynthesis of docosahexaenoic acid", "journal": "J Lipid Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11734571/" } ], "x": 311.33333333333337, "y": 1679.3508771929824, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2076371", "label": "GLCE epimerises more GlcA to IdoA as sulfate content rises", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:20198" ], "source_url": "https://reactome.org/content/detail/R-HSA-2076371", "summary": "GLCE epimerises more GlcA to IdoA as sulfate content rises is a Reactome release 97 curated reaction event. The source record connects HS(4)-PGs to HS(5)-PGs in Golgi membrane · Golgi lumen and cross-references RHEA:20198.", "equation": "HS(4)-PGs → HS(5)-PGs", "inputs": [ { "node_id": "R-HSA-2076491", "reactome_id": "R-HSA-2076491", "label": "HS(4)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2076461", "reactome_id": "R-HSA-2076461", "label": "HS(5)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "heparosan-N-sulfate-glucuronate 5-epimerase activity of GLCE dimer [Golgi membrane]", "activity_id": "2024110", "ec_numbers": [ "5.1.3.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:11274177", "title": "Characterization of the D-glucuronyl C5-epimerase involved in the biosynthesis of heparin and heparan sulfate", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11274177/" }, { "id": "PMID:11279150", "title": "Cloning, Golgi localization, and enzyme activity of the full-length heparin/heparan sulfate-glucuronic acid C5-epimerase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11279150/" } ], "x": 2660, "y": 912.9242424242424, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2076383", "label": "HS3ST1 sulfates GlcN at C3 in heparan sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi lumen", "compartment_ids": [ "GO:0005796" ], "rhea_ids": [ "RHEA:15462" ], "source_url": "https://reactome.org/content/detail/R-HSA-2076383", "summary": "HS3ST1 sulfates GlcN at C3 in heparan sulfate is a Reactome release 97 curated reaction event. The source record connects HS(3)-PGs, PAPS to PAP, HS(4)-PGs, H+ in Golgi lumen and cross-references RHEA:15462.", "equation": "HS(3)-PGs + PAPS → PAP + HS(4)-PGs + H+", "inputs": [ { "node_id": "R-HSA-2076297", "reactome_id": "R-HSA-2076297", "label": "HS(3)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076491", "reactome_id": "R-HSA-2076491", "label": "HS(4)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "[heparan sulfate]-glucosamine 3-sulfotransferase activity of HS3ST1 [Golgi lumen]", "activity_id": "2076328", "ec_numbers": [ "2.8.2.23" ], "accessions": [ "O14792" ] } ], "citations": [ { "id": "PMID:9346953", "title": "Molecular cloning and expression of mouse and human cDNAs encoding heparan sulfate D-glucosaminyl 3-O-sulfotransferase", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9346953/" } ], "x": 2660, "y": 865.7727272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2076392", "label": "EXT1:EXT2 transfers GlcA to heparan", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:20909" ], "source_url": "https://reactome.org/content/detail/R-HSA-2076392", "summary": "EXT1:EXT2 transfers GlcA to heparan is a Reactome release 97 curated reaction event. The source record connects UDP-GlcA, Heparan(2)-PGs to UDP, Heparan(3)-PGs, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:20909.", "equation": "UDP-GlcA + Heparan(2)-PGs → UDP + Heparan(3)-PGs + H+", "inputs": [ { "node_id": "R-ALL-1889970", "reactome_id": "R-ALL-1889970", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076412", "reactome_id": "R-HSA-2076412", "label": "Heparan(2)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076535", "reactome_id": "R-HSA-2076535", "label": "Heparan(3)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase activity of EXT1:EXT2 [Golgi membrane]", "activity_id": "2022849", "ec_numbers": [ "2.4.1.225" ], "accessions": [] } ], "citations": [ { "id": "PMID:11169766", "title": "Diminished levels of the putative tumor suppressor proteins EXT1 and EXT2 in exostosis chondrocytes", "journal": "Cell Motil Cytoskeleton", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11169766/" }, { "id": "PMID:10639137", "title": "The putative tumor suppressors EXT1 and EXT2 form a stable complex that accumulates in the Golgi apparatus and catalyzes the synthesis of heparan sulfate", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10639137/" }, { "id": "PMID:9463333", "title": "Mutations in the EXT1 and EXT2 genes in hereditary multiple exostoses", "journal": "Am J Hum Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9463333/" } ], "x": 2938.666666666667, "y": 865.7727272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2076419", "label": "HS6STs sulfate GlcN at C6 in heparan sulfate/heparin", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:56605" ], "source_url": "https://reactome.org/content/detail/R-HSA-2076419", "summary": "HS6STs sulfate GlcN at C6 in heparan sulfate/heparin is a Reactome release 97 curated reaction event. The source record connects HS(5)-PGs, PAPS to PAP, HS/HPIN-PGs, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:56605.", "equation": "HS(5)-PGs + PAPS → PAP + HS/HPIN-PGs + H+", "inputs": [ { "node_id": "R-HSA-2076461", "reactome_id": "R-HSA-2076461", "label": "HS(5)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076357", "reactome_id": "R-HSA-2076357", "label": "HS/HPIN-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "heparan sulfate 6-sulfotransferase activity of HS6STs [Golgi membrane]", "activity_id": "2076304", "ec_numbers": [ "2.8.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9535912", "title": "Molecular characterization and expression of heparan-sulfate 6-sulfotransferase. Complete cDNA cloning in human and partial cloning in Chinese hamster ovary cells", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9535912/" }, { "id": "PMID:12492399", "title": "Biosynthesis of heparan sulphate with diverse structures and functions: two alternatively spliced forms of human heparan sulphate 6-O-sulphotransferase-2 having different expression patterns and properties", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12492399/" }, { "id": "PMID:10644753", "title": "The occurrence of three isoforms of heparan sulfate 6-O-sulfotransferase having different specificities for hexuronic acid adjacent to the targeted N-sulfoglucosamine", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10644753/" }, { "id": "PMID:21700882", "title": "Heparan sulfate 6-O-sulfotransferase 1, a gene involved in extracellular sugar modifications, is mutated in patients with idiopathic hypogonadotrophic hypogonadism", "journal": "Proc Natl Acad Sci U S A", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21700882/" }, { "id": "PMID:30471091", "title": "A HS6ST2 gene variant associated with X-linked intellectual disability and severe myopia in two male twins", "journal": "Clin Genet", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30471091/" } ], "x": 2381.3333333333335, "y": 877.560606060606, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2076508", "label": "HS2ST1 trimer sulfates IdoA at C2 in heparan sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2076508", "summary": "HS2ST1 trimer sulfates IdoA at C2 in heparan sulfate is a Reactome release 97 curated reaction event. The source record connects HS(2)-PGs, PAPS to PAP, HS(3)-PGs, H+ in Golgi membrane · Golgi lumen.", "equation": "HS(2)-PGs + PAPS → PAP + HS(3)-PGs + H+", "inputs": [ { "node_id": "R-HSA-2076425", "reactome_id": "R-HSA-2076425", "label": "HS(2)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076297", "reactome_id": "R-HSA-2076297", "label": "HS(3)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "heparan sulfate 2-sulfotransferase activity of HS2ST1 trimer [Golgi membrane]", "activity_id": "2076430", "ec_numbers": [ "2.8.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:10677367", "title": "Expression of heparan sulphate L-iduronyl 2-O-sulphotransferase in human kidney 293 cells results in increased D-glucuronyl 2-O-sulphation", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10677367/" }, { "id": "PMID:20554947", "title": "Target selection of heparan sulfate hexuronic acid 2-O-sulfotransferase", "journal": "Glycobiology", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20554947/" }, { "id": "PMID:33159882", "title": "Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome Characterized by Developmental Delay and Corpus Callosum, Skeletal, and Renal Abnormalities", "journal": "Am J Hum Genet", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33159882/" } ], "x": 2381.3333333333335, "y": 1007.2272727272727, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2076611", "label": "HS3ST2-6 sulfate GlcN at C3 in heparan sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:15462" ], "source_url": "https://reactome.org/content/detail/R-HSA-2076611", "summary": "HS3ST2-6 sulfate GlcN at C3 in heparan sulfate is a Reactome release 97 curated reaction event. The source record connects HS(3)-PGs, PAPS to PAP, HS(4)-PGs, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:15462.", "equation": "HS(3)-PGs + PAPS → PAP + HS(4)-PGs + H+", "inputs": [ { "node_id": "R-HSA-2076297", "reactome_id": "R-HSA-2076297", "label": "HS(3)-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076491", "reactome_id": "R-HSA-2076491", "label": "HS(4)-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "[heparan sulfate]-glucosamine 3-sulfotransferase activity of HS3ST2-6 [Golgi membrane]", "activity_id": "2076583", "ec_numbers": [ "2.8.2.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:9988767", "title": "Multiple isoforms of heparan sulfate D-glucosaminyl 3-O-sulfotransferase. Isolation, characterization, and expression of human cDNAs and identification of distinct genomic loci", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9988767/" }, { "id": "PMID:12138164", "title": "Heparan sulfate 3-O-sulfotransferase isoform 5 generates both an antithrombin-binding site and an entry receptor for herpes simplex virus, type 1", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12138164/" }, { "id": "PMID:9346953", "title": "Molecular cloning and expression of mouse and human cDNAs encoding heparan sulfate D-glucosaminyl 3-O-sulfotransferase", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9346953/" } ], "x": 2660, "y": 877.560606060606, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2090037", "label": "IDUA hydrolyses Heparan sulfate chain(6)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2090037", "summary": "IDUA hydrolyses Heparan sulfate chain(6) is a Reactome release 97 curated reaction event. The source record connects H2O, IdoA-GlcNS3S- group to aldehydo-L-iduronic acid, GlcNS3S- group in lysosomal lumen.", "equation": "H2O + IdoA-GlcNS3S- group → aldehydo-L-iduronic acid + GlcNS3S- group", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090055", "reactome_id": "R-ALL-2090055", "label": "IdoA-GlcNS3S- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63809", "formula": "C35H56NO36S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1678617", "reactome_id": "R-ALL-1678617", "label": "aldehydo-L-iduronic acid", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:21338", "formula": "C6H9O7", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090070", "reactome_id": "R-ALL-2090070", "label": "GlcNS3S- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63810", "formula": "C29H48NO30S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-iduronidase activity of IDUA [lysosomal lumen]", "activity_id": "1678683", "ec_numbers": [ "3.2.1.76" ], "accessions": [ "P35475" ] } ], "citations": [ { "id": "PMID:1946389", "title": "Human alpha-L-iduronidase: cDNA isolation and expression", "journal": "Proc Natl Acad Sci U S A", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1946389/" }, { "id": "PMID:10466419", "title": "Mucopolysaccharidosis type I: characterization of novel mutations affecting alpha-L-iduronidase activity", "journal": "Clin Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10466419/" } ], "x": 2660, "y": 960.0757575757575, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2090038", "label": "NAGLU hydrolyses heparan chain(2)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2090038", "summary": "NAGLU hydrolyses heparan chain(2) is a Reactome release 97 curated reaction event. The source record connects H2O, GlcNAc- group to GlcNAc, -R in lysosomal lumen.", "equation": "H2O + GlcNAc- group → GlcNAc + -R", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090053", "reactome_id": "R-ALL-2090053", "label": "GlcNAc- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747401", "formula": "C31H50NO26", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1678743", "reactome_id": "R-ALL-1678743", "label": "GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17411", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9941448", "reactome_id": "R-ALL-9941448", "label": "-R", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:24433", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alpha-N-acetylglucosaminidase activity of NAGLU(59-743) [lysosomal lumen]", "activity_id": "1678739", "ec_numbers": [ "3.2.1.50" ], "accessions": [ "P54802" ] } ], "citations": [ { "id": "PMID:8776591", "title": "Cloning and expression of the gene involved in Sanfilippo B syndrome (mucopolysaccharidosis III B)", "journal": "Hum Mol Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8776591/" }, { "id": "PMID:16151907", "title": "Molecular defects in Sanfilippo syndrome type B (mucopolysaccharidosis IIIB)", "journal": "J Inherit Metab Dis", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16151907/" } ], "x": 2938.666666666667, "y": 960.0757575757575, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2090043", "label": "SGSH hydrolyses Heparan sulfate chain(7)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2090043", "summary": "SGSH hydrolyses Heparan sulfate chain(7) is a Reactome release 97 curated reaction event. The source record connects H2O, GlcNS3S- group to SO4(2-), GlcN- group in lysosomal lumen.", "equation": "2 H2O + GlcNS3S- group → 2 SO4(2-) + GlcN- group", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090070", "reactome_id": "R-ALL-2090070", "label": "GlcNS3S- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63810", "formula": "C29H48NO30S2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090045", "reactome_id": "R-ALL-2090045", "label": "GlcN- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63811", "formula": "C29H48NO24", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-sulfoglucosamine sulfohydrolase activity of SGSH:Ca2+ [lysosomal lumen]", "activity_id": "1678719", "ec_numbers": [ "3.10.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:7493035", "title": "Cloning of the sulphamidase gene and identification of mutations in Sanfilippo A syndrome", "journal": "Nat Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7493035/" }, { "id": "PMID:9285796", "title": "Novel mutations in Sanfilippo A syndrome: implications for enzyme function", "journal": "Hum Mol Genet", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9285796/" } ], "x": 2381.3333333333335, "y": 971.8636363636364, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2090079", "label": "GLB1 hydrolyses linker chain(2)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2090079", "summary": "GLB1 hydrolyses linker chain(2) is a Reactome release 97 curated reaction event. The source record connects H2O, linker chain(2) to Gal, D-xylose in lysosomal lumen.", "equation": "2 H2O + linker chain(2) → 2 Gal + D-xylose", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090075", "reactome_id": "R-ALL-2090075", "label": "linker chain(2)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:154964", "formula": "C17H30O15", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605637", "reactome_id": "R-ALL-1605637", "label": "Gal", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090063", "reactome_id": "R-ALL-2090063", "label": "D-xylose", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15936", "formula": "C5H10O5", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-galactosidase activity of BGAL [lysosomal lumen]", "activity_id": "1605789", "ec_numbers": [ "3.2.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:5822067", "title": "Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase", "journal": "Biochem J", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5822067/" }, { "id": "PMID:1907800", "title": "Human beta-galactosidase gene mutations in GM1-gangliosidosis: a common mutation among Japanese adult/chronic cases", "journal": "Am J Hum Genet", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1907800/" }, { "id": "PMID:1928092", "title": "Human beta-galactosidase gene mutations in morquio B disease", "journal": "Am J Hum Genet", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1928092/" } ], "x": 2660, "y": 971.8636363636364, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2090085", "label": "HGSNAT oligomer acetylates Heparan chain(1)", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal membrane · cytosol · lysosomal lumen", "compartment_ids": [ "GO:0005765", "GO:0005829", "GO:0043202" ], "rhea_ids": [ "RHEA:15126" ], "source_url": "https://reactome.org/content/detail/R-HSA-2090085", "summary": "HGSNAT oligomer acetylates Heparan chain(1) is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, GlcN- group to CoA-SH, GlcNAc- group, H+ in lysosomal membrane · cytosol · lysosomal lumen and cross-references RHEA:15126.", "equation": "Ac-CoA + GlcN- group → CoA-SH + GlcNAc- group + H+", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2090045", "reactome_id": "R-ALL-2090045", "label": "GlcN- group", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63811", "formula": "C29H48NO24", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2090053", "reactome_id": "R-ALL-2090053", "label": "GlcNAc- group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747401", "formula": "C31H50NO26", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428040", "reactome_id": "R-ALL-428040", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "heparan-alpha-glucosaminide N-acetyltransferase activity of HGSNAT oligomer [lysosomal membrane]", "activity_id": "1678732", "ec_numbers": [ "2.3.1.78" ], "accessions": [] } ], "citations": [ { "id": "PMID:20650889", "title": "Analysis of the biogenesis of heparan sulfate acetyl-CoA:alpha-glucosaminide N-acetyltransferase provides insights into the mechanism underlying its complete deficiency in mucopolysaccharidosis IIIC", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20650889/" }, { "id": "PMID:16960811", "title": "Identification of the gene encoding the enzyme deficient in mucopolysaccharidosis IIIC (Sanfilippo disease type C)", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16960811/" }, { "id": "PMID:17033958", "title": "Mutations in TMEM76* cause mucopolysaccharidosis IIIC (Sanfilippo C syndrome)", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17033958/" } ], "x": 2381.3333333333335, "y": 783.2575757575758, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-209738", "label": "VD3 binds GC", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "plasma membrane · cytosol · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-209738", "summary": "VD3 binds GC is a Reactome release 97 curated reaction event. The source record connects GC, VD3 to GC:VD3 in plasma membrane · cytosol · extracellular region.", "equation": "GC + VD3 → GC:VD3", "inputs": [ { "node_id": "R-HSA-209886", "reactome_id": "R-HSA-209886", "label": "GC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-209767", "reactome_id": "R-ALL-209767", "label": "VD3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28940", "formula": "C27H44O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-352339", "reactome_id": "R-HSA-352339", "label": "GC:VD3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of GC [extracellular region]", "activity_id": "9959976", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11799400", "title": "A structural basis for the unique binding features of the human vitamin D-binding protein", "journal": "Nat Struct Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11799400/" }, { "id": "PMID:27250744", "title": "Comparison of Two ELISA Methods and Mass Spectrometry for Measurement of Vitamin D-Binding Protein: Implications for the Assessment of Bioavailable Vitamin D Concentrations Across Genotypes", "journal": "J. Bone Miner. Res.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27250744/" } ], "x": 4451.333333333333, "y": 1748.128787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-209754", "label": "Photolytic cleavage and thermal isomerization of 7-dehydroCHOL", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-209754", "summary": "Photolytic cleavage and thermal isomerization of 7-dehydroCHOL is a Reactome release 97 curated reaction event. The source record connects 7-dehydroCHOL to VD3 in endoplasmic reticulum membrane.", "equation": "7-dehydroCHOL → VD3", "inputs": [ { "node_id": "R-ALL-6807057", "reactome_id": "R-ALL-6807057", "label": "7-dehydroCHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17759", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8963870", "reactome_id": "R-ALL-8963870", "label": "VD3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28940", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [], "x": 4730, "y": 1824.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-209760", "label": "Endocytic translocation of CUBN:GC:25(OH)D to lysosomal lumen", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · lysosomal lumen", "compartment_ids": [ "GO:0005829", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-209760", "summary": "Endocytic translocation of CUBN:GC:25(OH)D to lysosomal lumen is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CUBN:GC:25(OH)D to CUBN:GC:25(OH)D in cytosol · lysosomal lumen.", "equation": "CUBN:GC:25(OH)D → CUBN:GC:25(OH)D", "inputs": [ { "node_id": "R-HSA-350092", "reactome_id": "R-HSA-350092", "label": "CUBN:GC:25(OH)D", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-350085", "reactome_id": "R-HSA-350085", "label": "CUBN:GC:25(OH)D", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9821970", "title": "Autocatalytic activation of human legumain at aspartic acid residues", "journal": "FEBS Lett", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9821970/" }, { "id": "PMID:11085925", "title": "Activation of human prolegumain by cleavage at a C-terminal asparagine residue", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11085925/" } ], "x": 4451.333333333333, "y": 1913.159090909091, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-209765", "label": "CYP24A1 hydroxylates 1,25(OH)2D, inactivating it", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "mitochondrial inner membrane · cytosol", "compartment_ids": [ "GO:0005743", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-209765", "summary": "CYP24A1 hydroxylates 1,25(OH)2D, inactivating it is a Reactome release 97 curated reaction event. The source record connects 1,25(OH)2D, NADPH, H+ to CTA, NADP+, H2O in mitochondrial inner membrane · cytosol.", "equation": "1,25(OH)2D + NADPH + H+ + O2 → CTA + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-209800", "reactome_id": "R-ALL-209800", "label": "1,25(OH)2D", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17823", "formula": "C27H44O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-350159", "reactome_id": "R-ALL-350159", "label": "CTA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:47828", "formula": "C23H34O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity of CYP24A1 [mitochondrial inner membrane]", "activity_id": "215644", "ec_numbers": null, "accessions": [ "Q07973" ] } ], "citations": [ { "id": "PMID:8506296", "title": "Isolation and expression of human 1,25-dihydroxyvitamin D3 24-hydroxylase cDNA", "journal": "Proc Natl Acad Sci U S A", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8506296/" }, { "id": "PMID:8679605", "title": "Human 25-hydroxyvitamin D3-24-hydroxylase, a multicatalytic enzyme", "journal": "Biochemistry", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8679605/" }, { "id": "PMID:11012668", "title": "Dual metabolic pathway of 25-hydroxyvitamin D3 catalyzed by human CYP24", "journal": "Eur J Biochem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11012668/" } ], "x": 4451.333333333333, "y": 1677.4015151515152, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-209766", "label": "25(OH)D translocates from lysosomal lumen to cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · lysosomal lumen", "compartment_ids": [ "GO:0005829", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-209766", "summary": "25(OH)D translocates from lysosomal lumen to cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 25(OH)D to 25(OH)D in cytosol · lysosomal lumen.", "equation": "25(OH)D → 25(OH)D", "inputs": [ { "node_id": "R-ALL-350136", "reactome_id": "R-ALL-350136", "label": "25(OH)D", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17933", "formula": "C27H44O2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-209804", "reactome_id": "R-ALL-209804", "label": "25(OH)D", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17933", "formula": "C27H44O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:26865365", "title": "The human HSP70 family of chaperones: where do we stand?", "journal": "Cell Stress Chaperones", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26865365/" } ], "x": 5008.666666666667, "y": 1854.219696969697, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-209845", "label": "CYP2R1 25-hydroxylates VD3 to 25(OH)D", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-209845", "summary": "CYP2R1 25-hydroxylates VD3 to 25(OH)D is a Reactome release 97 curated reaction event. The source record connects VD3, O2, H+ to 25(OH)D, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "VD3 + O2 + H+ + NADPH → 25(OH)D + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-8963870", "reactome_id": "R-ALL-8963870", "label": "VD3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28940", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8963879", "reactome_id": "R-ALL-8963879", "label": "25(OH)D", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17933", "formula": "C27H44O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "vitamin D3 25-hydroxylase activity of CYP2R1 [endoplasmic reticulum membrane]", "activity_id": "209900", "ec_numbers": null, "accessions": [ "Q6VVX0" ] } ], "citations": [ { "id": "PMID:15465040", "title": "Metabolism of vitamin D by human microsomal CYP2R1", "journal": "Biochem Biophys Res Commun", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15465040/" }, { "id": "PMID:12867411", "title": "De-orphanization of cytochrome P450 2R1: a microsomal vitamin D 25-hydroxilase", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12867411/" } ], "x": 4730, "y": 1677.4015151515152, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-209868", "label": "CYP27B1 hydroxylates 25(OH)D to 1,25(OH)2D", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "biological-oxidations", "phase-one", "steroids" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-209868", "summary": "CYP27B1 hydroxylates 25(OH)D to 1,25(OH)2D is a Reactome release 97 curated reaction event. The source record connects 25(OH)D, H+, NADPH to 1,25(OH)2D, H2O, NADP+ in cytosol · mitochondrial outer membrane.", "equation": "25(OH)D + H+ + NADPH + O2 → 1,25(OH)2D + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-209804", "reactome_id": "R-ALL-209804", "label": "25(OH)D", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17933", "formula": "C27H44O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-209800", "reactome_id": "R-ALL-209800", "label": "1,25(OH)2D", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17823", "formula": "C27H44O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "calcidiol 1-monooxygenase activity of CYP27B1(?-508) [mitochondrial outer membrane]", "activity_id": "209953", "ec_numbers": [ "1.14.15.18" ], "accessions": [ "O15528" ] } ], "citations": [ { "id": "PMID:11856765", "title": "Synthesis of 1,25-dihydroxyvitamin D(3) by human endothelial cells is regulated by inflammatory cytokines: a novel autocrine determinant of vascular cell adhesion", "journal": "J Am Soc Nephrol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11856765/" }, { "id": "PMID:12855575", "title": "Regulation of 25-hydroxyvitamin D3-1 alpha-hydroxylase and production of 1 alpha,25-dihydroxyvitamin D3 by human dendritic cells", "journal": "Blood", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12855575/" }, { "id": "PMID:10518789", "title": "Enzymatic properties of human 25-hydroxyvitamin D3 1alpha-hydroxylase coexpression with adrenodoxin and NADPH-adrenodoxin reductase in Escherichia coli", "journal": "Eur. J. Biochem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10518789/" } ], "x": 5008.666666666667, "y": 1677.4015151515152, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-209944", "label": "25(OH)D binds GC", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-209944", "summary": "25(OH)D binds GC is a Reactome release 97 curated reaction event. The source record connects 25(OH)D, GC to GC:25(OH)D in extracellular region.", "equation": "25(OH)D + GC → GC:25(OH)D", "inputs": [ { "node_id": "R-ALL-209906", "reactome_id": "R-ALL-209906", "label": "25(OH)D", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17933", "formula": "C27H44O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-209886", "reactome_id": "R-HSA-209886", "label": "GC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-209892", "reactome_id": "R-HSA-209892", "label": "GC:25(OH)D", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of GC [extracellular region]", "activity_id": "9959976", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11799400", "title": "A structural basis for the unique binding features of the human vitamin D-binding protein", "journal": "Nat Struct Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11799400/" } ], "x": 4730, "y": 1748.128787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2105001", "label": "HEXA cleaves the terminal GalNAc from DS", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2105001", "summary": "HEXA cleaves the terminal GalNAc from DS is a Reactome release 97 curated reaction event. The source record connects H2O, ChEBI:63516 chain to GalNAc, IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl in lysosomal lumen.", "equation": "H2O + ChEBI:63516 chain → GalNAc + IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2065269", "reactome_id": "R-ALL-2065269", "label": "ChEBI:63516 chain", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747395", "formula": "C45H69N2O40S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605658", "reactome_id": "R-ALL-1605658", "label": "GalNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28037", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2105025", "reactome_id": "R-ALL-2105025", "label": "IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63873", "formula": "C37H59NO35S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXA [lysosomal lumen]", "activity_id": "1605773", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:2971395", "title": "Oligosaccharide structure and amino acid sequence of the major glycopeptides of mature human beta-hexosaminidase", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2971395/" } ], "x": 2938.666666666667, "y": 971.8636363636364, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211873", "label": "CYP4F2, 4F3 20-hydroxylate LTB4", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "biological-oxidations", "fatty-acid-metabolism", "phase-one" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:22177" ], "source_url": "https://reactome.org/content/detail/R-HSA-211873", "summary": "CYP4F2, 4F3 20-hydroxylate LTB4 is a Reactome release 97 curated reaction event. The source record connects LTB4, NADPH, H+ to 20OH-LTB4, H2O, NADP+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:22177.", "equation": "LTB4 + NADPH + H+ + O2 → 20OH-LTB4 + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-266056", "reactome_id": "R-ALL-266056", "label": "LTB4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57461", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2161636", "reactome_id": "R-ALL-2161636", "label": "20OH-LTB4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57460", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "leukotriene-B4 20-monooxygenase activity of Cytochrome P450 (CYP4F2/4F3 based) [endoplasmic reticulum membrane]", "activity_id": "2162138", "ec_numbers": [ "1.14.14.94" ], "accessions": [] } ], "citations": [ { "id": "PMID:9799565", "title": "Role of human CYP4F2 in hepatic catabolism of the proinflammatory agent leukotriene B4", "journal": "Arch Biochem Biophys", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9799565/" }, { "id": "PMID:9539102", "title": "Human leukotriene B4 omega-hydroxylase (CYP4F3) gene: molecular cloning and chromosomal localization", "journal": "DNA Cell Biol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9539102/" } ], "x": 868.6666666666667, "y": 1643.140350877193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211874", "label": "CYP2S1 4-hydroxylates atRA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211874", "summary": "CYP2S1 4-hydroxylates atRA is a Reactome release 97 curated reaction event. The source record connects atRA, NADPH, H+ to 4OH-atRA, NADP+, H2O in smooth endoplasmic reticulum.", "equation": "atRA + NADPH + H+ + O2 → 4OH-atRA + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-215573", "reactome_id": "R-ALL-215573", "label": "atRA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15367", "formula": "C20H28O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-215584", "reactome_id": "R-ALL-215584", "label": "4OH-atRA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63795", "formula": "C20H28O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "retinoic acid 4-hydroxylase activity of CYP2S1 [endoplasmic reticulum membrane]", "activity_id": "216562", "ec_numbers": null, "accessions": [ "Q96SQ9" ] } ], "citations": [ { "id": "PMID:12711469", "title": "Cutaneous expression of cytochrome P450 CYP2S1: individuality in regulation by therapeutic agents for psoriasis and other skin diseases", "journal": "Lancet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12711469/" } ], "x": 8018.666666666667, "y": 2951.735294117647, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211881", "label": "Coumarin is 7-hydroxylated by CYP2A13", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen · smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005789", "GO:0005788", "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211881", "summary": "Coumarin is 7-hydroxylated by CYP2A13 is a Reactome release 97 curated reaction event. The source record connects COUM, O2, NADPH to HCOU, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen · smooth endoplasmic reticulum.", "equation": "COUM + O2 + NADPH + H+ → HCOU + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-76328", "reactome_id": "R-ALL-76328", "label": "COUM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28794", "formula": "C9H6O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76452", "reactome_id": "R-ALL-76452", "label": "HCOU", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27510", "formula": "C9H6O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "coumarin 7-hydroxylase activity of Cytochrome P450 (CYP2A13 based) [endoplasmic reticulum membrane]", "activity_id": "211854", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15196988", "title": "Identification of Val117 and Arg372 as critical amino acid residues for the activity difference between human CYP2A6 and CYP2A13 in coumarin 7-hydroxylation", "journal": "Arch Biochem Biophys", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15196988/" } ], "x": 7461.333333333333, "y": 2959.3627450980393, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211882", "label": "CYP3A7 can 6beta-hydroxylate testosterone", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211882", "summary": "CYP3A7 can 6beta-hydroxylate testosterone is a Reactome release 97 curated reaction event. The source record connects TES, NADPH, H+ to 6BHT, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "TES + NADPH + H+ + O2 → 6BHT + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-30321", "reactome_id": "R-ALL-30321", "label": "TES", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17347", "formula": "C19H28O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-213144", "reactome_id": "R-ALL-213144", "label": "6BHT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:34477", "formula": "C19H28O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP3A7 based) [endoplasmic reticulum membrane]", "activity_id": "213160", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9644715", "title": "Steroid hydroxylation by human fetal CYP3A7 and human NADPH-cytochrome P450 reductase coexpressed in insect cells using baculovirus", "journal": "Res Commun Mol Pathol Pharmacol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9644715/" } ], "x": 7740, "y": 2959.3627450980393, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211904", "label": "CYP4F12 18-hydroxylates ARA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211904", "summary": "CYP4F12 18-hydroxylates ARA is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to 18OH-ARA, H2O, NADP+ in smooth endoplasmic reticulum.", "equation": "AA + O2 + NADPH + H+ → 18OH-ARA + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-352318", "reactome_id": "R-ALL-352318", "label": "18OH-ARA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63590", "formula": "C20H31O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP4F12 based) [endoplasmic reticulum membrane]", "activity_id": "215063", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11162607", "title": "cDna cloning and expression of CYP4F12, a novel human cytochrome P450", "journal": "Biochem Biophys Res Commun", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11162607/" } ], "x": 8018.666666666667, "y": 2959.3627450980393, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211910", "label": "CYP2C8 inactivates paclitaxel by 6alpha-hydroxylation", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211910", "summary": "CYP2C8 inactivates paclitaxel by 6alpha-hydroxylation is a Reactome release 97 curated reaction event. The source record connects PTXL, O2, NADPH to HPTXL, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "PTXL + O2 + NADPH + H+ → HPTXL + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-211906", "reactome_id": "R-ALL-211906", "label": "PTXL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:45863", "formula": "C47H51NO14", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-211894", "reactome_id": "R-ALL-211894", "label": "HPTXL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63859", "formula": "C47H51NO15", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2C8 based) [endoplasmic reticulum membrane]", "activity_id": "211888", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9842986", "title": "Human liver microsomal metabolism of paclitaxel and drug interactions", "journal": "Eur J Drug Metab Pharmacokinet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9842986/" } ], "x": 7461.333333333333, "y": 2966.9901960784314, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211919", "label": "CYP4F8 19-hydroxylates PGH2", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211919", "summary": "CYP4F8 19-hydroxylates PGH2 is a Reactome release 97 curated reaction event. The source record connects PGH2, NADPH, H+ to NADP+, H2O, 19OH-PGH2 in smooth endoplasmic reticulum.", "equation": "PGH2 + NADPH + H+ + O2 → NADP+ + H2O + 19OH-PGH2", "inputs": [ { "node_id": "R-ALL-30138", "reactome_id": "R-ALL-30138", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-215545", "reactome_id": "R-ALL-215545", "label": "19OH-PGH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63912", "formula": "C20H32O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP4F8 based) [endoplasmic reticulum membrane]", "activity_id": "215524", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10791960", "title": "Identification of CYP4F8 in human seminal vesicles as a prominent 19-hydroxylase of prostaglandin endoperoxides", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10791960/" }, { "id": "PMID:10405341", "title": "Gene expression of a novel cytochrome P450 of the CYP4F subfamily in human seminal vesicles", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10405341/" } ], "x": 7740, "y": 2966.9901960784314, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211923", "label": "CYP26C1 4-hydroxylates 9cRA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211923", "summary": "CYP26C1 4-hydroxylates 9cRA is a Reactome release 97 curated reaction event. The source record connects 9cRA, NADPH, H+ to 4OH-9cRA, NADP+, H2O in endoplasmic reticulum membrane · cytosol.", "equation": "9cRA + NADPH + H+ + O2 → 4OH-9cRA + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-5362520", "reactome_id": "R-ALL-5362520", "label": "9cRA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:50648", "formula": "C20H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5362524", "reactome_id": "R-ALL-5362524", "label": "4OH-9cRA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63802", "formula": "C20H28O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "retinoic acid 4-hydroxylase activity of CYP26C1 [endoplasmic reticulum membrane]", "activity_id": "215633", "ec_numbers": null, "accessions": [ "Q6V0L0" ] } ], "citations": [ { "id": "PMID:14532297", "title": "A novel human cytochrome P450, CYP26C1, involved in metabolism of 9-cis and all-trans isomers of retinoic acid", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14532297/" } ], "x": 8018.666666666667, "y": 2966.9901960784314, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211924", "label": "CYP4B1 12-hydroxylates ARA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211924", "summary": "CYP4B1 12-hydroxylates ARA is a Reactome release 97 curated reaction event. The source record connects AA, H+, NADPH to 12-HETE, NADP+, H2O in smooth endoplasmic reticulum.", "equation": "AA + H+ + NADPH + O2 → 12-HETE + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-215075", "reactome_id": "R-ALL-215075", "label": "12-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:19138", "formula": "C20H32O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP4B1 based) [endoplasmic reticulum membrane]", "activity_id": "215079", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15006160", "title": "Retinoic acid induces corneal epithelial CYP4B1 gene expression and stimulates the synthesis of inflammatory 12-hydroxyeicosanoids", "journal": "J Ocul Pharmacol Ther", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15006160/" } ], "x": 7461.333333333333, "y": 2974.6176470588234, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211929", "label": "CYP2C19 5-hydroxylates omeprazole", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211929", "summary": "CYP2C19 5-hydroxylates omeprazole is a Reactome release 97 curated reaction event. The source record connects Losec, NADPH, H+ to HOMZ, NADP+, H2O in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "Losec + NADPH + H+ + O2 → HOMZ + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-211892", "reactome_id": "R-ALL-211892", "label": "Losec", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:7772", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-211944", "reactome_id": "R-ALL-211944", "label": "HOMZ", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63840", "formula": "C17H19N3O4S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2C19 based) [endoplasmic reticulum membrane]", "activity_id": "211869", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8647857", "title": "Identification of residues 99, 220, and 221 of human cytochrome P450 2C19 as key determinants of omeprazole activity", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8647857/" } ], "x": 7740, "y": 2974.6176470588234, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211948", "label": "CYP3A4 can N-demethylate loperamide", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211948", "summary": "CYP3A4 can N-demethylate loperamide is a Reactome release 97 curated reaction event. The source record connects LPAM, H+, NADPH to N-demethylated loperamide, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "LPAM + H+ + NADPH + O2 → N-demethylated loperamide + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-213155", "reactome_id": "R-ALL-213155", "label": "LPAM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:6532", "formula": "C29H33ClN2O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-213151", "reactome_id": "R-ALL-213151", "label": "N-demethylated loperamide", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64043", "formula": "C28H31ClN2O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYPIIIA4 based) [endoplasmic reticulum membrane]", "activity_id": "213172", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15365656", "title": "Identification of cytochrome P450 isoforms involved in the metabolism of loperamide in human liver microsomes", "journal": "Eur J Clin Pharmacol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15365656/" } ], "x": 8018.666666666667, "y": 2974.6176470588234, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211950", "label": "CYP24A1 24-hydroxylates CTL", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211950", "summary": "CYP24A1 24-hydroxylates CTL is a Reactome release 97 curated reaction event. The source record connects CTL, NADPH, H+ to CTLA, NADP+, H2O in mitochondrial matrix · mitochondrial inner membrane.", "equation": "CTL + NADPH + H+ + O2 → CTLA + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-215607", "reactome_id": "R-ALL-215607", "label": "CTL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17823", "formula": "C27H44O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-215622", "reactome_id": "R-ALL-215622", "label": "CTLA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63911", "formula": "C27H44O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity of CYP24A1 [mitochondrial inner membrane]", "activity_id": "215644", "ec_numbers": null, "accessions": [ "Q07973" ] } ], "citations": [ { "id": "PMID:17646648", "title": "Single A326G mutation converts human CYP24A1 from 25-OH-D3-24-hydroxylase into -23-hydroxylase, generating 1alpha,25-(OH)2D3-26,23-lactone", "journal": "Proc Natl Acad Sci U S A", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17646648/" }, { "id": "PMID:8679605", "title": "Human 25-hydroxyvitamin D3-24-hydroxylase, a multicatalytic enzyme", "journal": "Biochemistry", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8679605/" }, { "id": "PMID:11012668", "title": "Dual metabolic pathway of 25-hydroxyvitamin D3 catalyzed by human CYP24", "journal": "Eur J Biochem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11012668/" } ], "x": 7461.333333333333, "y": 2982.245098039216, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211951", "label": "CYP1B1 4-hydroxylates EST17b", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:47281" ], "source_url": "https://reactome.org/content/detail/R-HSA-211951", "summary": "CYP1B1 4-hydroxylates EST17b is a Reactome release 97 curated reaction event. The source record connects EST17b, H+, NADPH to 4OH-EST17b, H2O, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:47281.", "equation": "EST17b + H+ + NADPH + O2 → 4OH-EST17b + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-193132", "reactome_id": "R-ALL-193132", "label": "EST17b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16469", "formula": "C18H24O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-211973", "reactome_id": "R-ALL-211973", "label": "4OH-EST17b", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62845", "formula": "C18H24O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen of CYP1B1 [e…", "activity_id": "211893", "ec_numbers": [ "1.14.13" ], "accessions": [ "Q16678" ] } ], "citations": [ { "id": "PMID:11555828", "title": "Role of human cytochrome P450 1A1, 1A2, 1B1, and 3A4 in the 2-, 4-, and 16alpha-hydroxylation of 17beta-estradiol", "journal": "Metabolism", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11555828/" } ], "x": 8018.666666666667, "y": 2814.4411764705883, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211959", "label": "CYP3A43 6b-hydroxylates TEST", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211959", "summary": "CYP3A43 6b-hydroxylates TEST is a Reactome release 97 curated reaction event. The source record connects TES, NADPH, H+ to H2O, NADP+, 6BHT in smooth endoplasmic reticulum.", "equation": "TES + NADPH + H+ + O2 → H2O + NADP+ + 6BHT", "inputs": [ { "node_id": "R-ALL-30321", "reactome_id": "R-ALL-30321", "label": "TES", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17347", "formula": "C19H28O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-213144", "reactome_id": "R-ALL-213144", "label": "6BHT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:34477", "formula": "C19H28O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP3A43 [endoplasmic reticulum membrane]", "activity_id": "216569", "ec_numbers": null, "accessions": [ "Q9HB55" ] } ], "citations": [ { "id": "PMID:11160876", "title": "cDNA cloning and initial characterization of CYP3A43, a novel human cytochrome P450", "journal": "Mol Pharmacol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11160876/" }, { "id": "PMID:15548719", "title": "CYP3A4, CYP3A5, and CYP3A43 genotypes and haplotypes in the etiology and severity of prostate cancer", "journal": "Cancer Res", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15548719/" } ], "x": 7740, "y": 2982.245098039216, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211960", "label": "CYP2U1 19-hydroxylates ARA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211960", "summary": "CYP2U1 19-hydroxylates ARA is a Reactome release 97 curated reaction event. The source record connects AA, NADPH, H+ to 19HETE, H2O, NADP+ in smooth endoplasmic reticulum.", "equation": "AA + NADPH + H+ + O2 → 19HETE + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-216620", "reactome_id": "R-ALL-216620", "label": "19HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76627", "formula": "C20H31O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP2U1 [endoplasmic reticulum membrane]", "activity_id": "216595", "ec_numbers": null, "accessions": [ "Q7Z449" ] } ], "citations": [ { "id": "PMID:14660610", "title": "CYP2U1, a novel human thymus- and brain-specific cytochrome P450, catalyzes omega- and (omega-1)-hydroxylation of fatty acids", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14660610/" } ], "x": 8018.666666666667, "y": 2982.245098039216, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211962", "label": "CYP4F11 16-hydroxylates 3OH-PALM", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211962", "summary": "CYP4F11 16-hydroxylates 3OH-PALM is a Reactome release 97 curated reaction event. The source record connects 3OH-PALM, NADPH, H+ to 3,16DOH-PALM, NADP+, H2O in smooth endoplasmic reticulum.", "equation": "3OH-PALM + NADPH + H+ + O2 → 3,16DOH-PALM + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-216621", "reactome_id": "R-ALL-216621", "label": "3OH-PALM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37248", "formula": "C16H32O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-216612", "reactome_id": "R-ALL-216612", "label": "3,16DOH-PALM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63904", "formula": "C16H31O4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP4F11 based) [endoplasmic reticulum membrane]", "activity_id": "216629", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18065749", "title": "Omega oxidation of 3-hydroxy fatty acids by the human CYP4F gene subfamily enzyme CYP4F11", "journal": "J Lipid Res", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18065749/" } ], "x": 7461.333333333333, "y": 2989.872549019608, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211966", "label": "CYP2D6 4-hydroxylates debrisoquine", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211966", "summary": "CYP2D6 4-hydroxylates debrisoquine is a Reactome release 97 curated reaction event. The source record connects DSQ, O2, NADPH to HDBQ, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "DSQ + O2 + NADPH + H+ → HDBQ + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-211993", "reactome_id": "R-ALL-211993", "label": "DSQ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:34665", "formula": "C10H13N3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-211952", "reactome_id": "R-ALL-211952", "label": "HDBQ", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63800", "formula": "C10H13N3O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom…", "activity_id": "212002", "ec_numbers": [ "1.14.14.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:7903454", "title": "Inherited amplification of an active gene in the cytochrome P450 CYP2D locus as a cause of ultrarapid metabolism of debrisoquine", "journal": "Proc Natl Acad Sci U S A", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7903454/" }, { "id": "PMID:6220203", "title": "Substrate specificity of the form of cytochrome P-450 catalyzing the 4-hydroxylation of debrisoquine in man", "journal": "Mol Pharmacol", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6220203/" } ], "x": 7740, "y": 2989.872549019608, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211968", "label": "CYP2W1 oxidises INDOL", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211968", "summary": "CYP2W1 oxidises INDOL is a Reactome release 97 curated reaction event. The source record connects INDOL, NADPH, H+ to Oxindole, NADP+, H2O in smooth endoplasmic reticulum.", "equation": "INDOL + NADPH + H+ + O2 → Oxindole + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-216547", "reactome_id": "R-ALL-216547", "label": "INDOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16881", "formula": "C8H7N", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-216598", "reactome_id": "R-ALL-216598", "label": "Oxindole", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:31697", "formula": "C8H7NO", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2W1 based) [endoplasmic reticulum membrane]", "activity_id": "216599", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:16677611", "title": "Enzymatic properties of human CYP2W1 expressed in Escherichia coli", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16677611/" } ], "x": 8018.666666666667, "y": 2989.872549019608, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211983", "label": "CYP2J2 oxidises ARA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211983", "summary": "CYP2J2 oxidises ARA is a Reactome release 97 curated reaction event. The source record connects AA, NADPH, H+ to cis-EET, NADP+, H2O in smooth endoplasmic reticulum.", "equation": "AA + NADPH + H+ + O2 → cis-EET + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-215062", "reactome_id": "R-ALL-215062", "label": "cis-EET", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "arachidonate epoxygenase activity of Cytochrome P450 (CYP2J2 based) [endoplasmic reticulum membrane]", "activity_id": "215088", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8913342", "title": "CYP2J subfamily P450s in the lung: expression, localization, and potential functional significance", "journal": "Mol Pharmacol", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8913342/" }, { "id": "PMID:8631948", "title": "Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631948/" } ], "x": 7461.333333333333, "y": 3050.892156862745, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211988", "label": "CYP2C9 inactivates tolbutamide by 4methyl-hydroxylation", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211988", "summary": "CYP2C9 inactivates tolbutamide by 4methyl-hydroxylation is a Reactome release 97 curated reaction event. The source record connects O2, NADPH, H+ to HTOLB, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "O2 + NADPH + H+ + TBM → HTOLB + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9838902", "reactome_id": "R-ALL-9838902", "label": "TBM", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "ChemicalDrug", "currency": false } ], "outputs": [ { "node_id": "R-ALL-211937", "reactome_id": "R-ALL-211937", "label": "HTOLB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63799", "formula": "C12H18N2O4S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2C9 based) [endoplasmic reticulum membrane]", "activity_id": "211992", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:2025243", "title": "Tolbutamide and phenytoin hydroxylations by cDNA-expressed human liver cytochrome P4502C9", "journal": "Biochem Biophys Res Commun", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2025243/" } ], "x": 7740, "y": 3050.892156862745, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-211991", "label": "Cyclophosphamide is 4-hydroxylated by CYP2B6", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-211991", "summary": "Cyclophosphamide is 4-hydroxylated by CYP2B6 is a Reactome release 97 curated reaction event. The source record connects CPM, O2, NADPH to HCPM, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "CPM + O2 + NADPH + H+ → HCPM + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-211977", "reactome_id": "R-ALL-211977", "label": "CPM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:4026", "formula": "C7H15Cl2N2O2P.H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-211967", "reactome_id": "R-ALL-211967", "label": "HCPM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:1864", "formula": "C7H15Cl2N2O3P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2B6 based) [endoplasmic reticulum membrane]", "activity_id": "211938", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12629583", "title": "Role of polymorphic human CYP2B6 in cyclophosphamide bioactivation", "journal": "Pharmacogenomics J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12629583/" } ], "x": 7461.333333333333, "y": 2997.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-212004", "label": "CYP2C18 initiates bioactivation of phenytoin by 4-hydroxylation", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-212004", "summary": "CYP2C18 initiates bioactivation of phenytoin by 4-hydroxylation is a Reactome release 97 curated reaction event. The source record connects Phenytoin, O2, NADPH to HPHT, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "Phenytoin + O2 + NADPH + H+ → HPHT + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-211939", "reactome_id": "R-ALL-211939", "label": "Phenytoin", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:8107", "formula": "C15H12N2O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-211927", "reactome_id": "R-ALL-211927", "label": "HPHT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63804", "formula": "C15H12N2O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2C18 based) [endoplasmic reticulum membrane]", "activity_id": "211871", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:16359177", "title": "P450 2C18 catalyzes the metabolic bioactivation of phenytoin", "journal": "Chem Res Toxicol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16359177/" } ], "x": 7740, "y": 2997.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-212005", "label": "CYP2F1 dehydrogenates 3-methylindole", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-212005", "summary": "CYP2F1 dehydrogenates 3-methylindole is a Reactome release 97 curated reaction event. The source record connects MIND, H+, NADPH to 3MEIN, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "MIND + H+ + NADPH + O2 → 3MEIN + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-213162", "reactome_id": "R-ALL-213162", "label": "MIND", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:9171", "formula": "C9H9N", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-213163", "reactome_id": "R-ALL-213163", "label": "3MEIN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63905", "formula": "C9H7N", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2F1 based) [endoplasmic reticulum membrane]", "activity_id": "213146", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10383923", "title": "Specific dehydrogenation of 3-methylindole and epoxidation of naphthalene by recombinant human CYP2F1 expressed in lymphoblastoid cells", "journal": "Drug Metab Dispos", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10383923/" } ], "x": 8018.666666666667, "y": 2997.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-212007", "label": "CYP26A1,B1 4-hydroxylate atRA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-212007", "summary": "CYP26A1,B1 4-hydroxylate atRA is a Reactome release 97 curated reaction event. The source record connects atRA, NADPH, H+ to 4OH-atRA, H2O, NADP+ in endoplasmic reticulum membrane.", "equation": "atRA + NADPH + H+ + O2 → 4OH-atRA + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-215573", "reactome_id": "R-ALL-215573", "label": "atRA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15367", "formula": "C20H28O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-215584", "reactome_id": "R-ALL-215584", "label": "4OH-atRA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63795", "formula": "C20H28O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "retinoic acid 4-hydroxylase activity of CYP26A1,B1,C1 [endoplasmic reticulum membrane]", "activity_id": "5362526", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9716180", "title": "Human retinoic acid (RA) 4-hydroxylase (CYP26) is highly specific for all-trans-RA and can be induced through RA receptors in human breast and colon carcinoma cells", "journal": "Cell Growth Differ", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9716180/" }, { "id": "PMID:10823918", "title": "Identification of the human cytochrome P450, P450RAI-2, which is predominantly expressed in the adult cerebellum and is responsible for all-trans-retinoic acid metabolism", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10823918/" } ], "x": 7461.333333333333, "y": 3005.127450980392, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-213175", "label": "CYP3A4,5 oxidise AFB1 to AFXBO", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-213175", "summary": "CYP3A4,5 oxidise AFB1 to AFXBO is a Reactome release 97 curated reaction event. The source record connects AFB1, O2, NADPH to AFXBO, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "AFB1 + O2 + NADPH + H+ → AFXBO + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-156522", "reactome_id": "R-ALL-156522", "label": "AFB1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2504", "formula": "C17H12O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-156523", "reactome_id": "R-ALL-156523", "label": "AFXBO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30725", "formula": "C17H12O7", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP3A5 based) [endoplasmic reticulum membrane]", "activity_id": "213161", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15454734", "title": "Increased levels of aflatoxin-albumin adducts are associated with CYP3A5 polymorphisms in The Gambia, West Africa", "journal": "Pharmacogenetics", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15454734/" }, { "id": "PMID:8975785", "title": "The kinetics of aflatoxin B1 oxidation by human cDNA-expressed and human liver microsomal cytochromes P450 1A2 and 3A4", "journal": "Toxicol. Appl. Pharmacol.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8975785/" } ], "x": 7740, "y": 3005.127450980392, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2142859", "label": "Growing HA is extruded from the cell by ABCC5", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane · cytosol · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2142859", "summary": "Growing HA is extruded from the cell by ABCC5 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects HA polymer to HA in plasma membrane · cytosol · extracellular region.", "equation": "HA polymer → HA", "inputs": [ { "node_id": "R-ALL-2142896", "reactome_id": "R-ALL-2142896", "label": "HA polymer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Polymer", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false } ], "catalysts": [ { "label": "transmembrane transporter activity of ABCC5 [plasma membrane]", "activity_id": "2142862", "ec_numbers": null, "accessions": [ "O15440" ] } ], "citations": [ { "id": "PMID:17540771", "title": "Hyaluronan export by the ABC transporter MRP5 and its modulation by intracellular cGMP", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17540771/" }, { "id": "PMID:25699059", "title": "Hyaluronan synthase 1: a mysterious enzyme with unexpected functions", "journal": "Front Immunol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25699059/" } ], "x": 2381.3333333333335, "y": 1042.590909090909, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-215526", "label": "CYP4F3 20-hydroxylates LTB4", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-215526", "summary": "CYP4F3 20-hydroxylates LTB4 is a Reactome release 97 curated reaction event. The source record connects LTB4, NADPH, H+ to 20OH-LTB4, H2O, NADP+ in smooth endoplasmic reticulum.", "equation": "LTB4 + NADPH + H+ + O2 → 20OH-LTB4 + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-215557", "reactome_id": "R-ALL-215557", "label": "LTB4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57461", "formula": "C20H31O4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-571314", "reactome_id": "R-ALL-571314", "label": "20OH-LTB4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57460", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP4F3 based) [endoplasmic reticulum membrane]", "activity_id": "215534", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9539102", "title": "Human leukotriene B4 omega-hydroxylase (CYP4F3) gene: molecular cloning and chromosomal localization", "journal": "DNA Cell Biol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9539102/" } ], "x": 8018.666666666667, "y": 3005.127450980392, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2160492", "label": "IL4I1:FAD oxidises L-Phe to kPPV", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [ "RHEA:61241" ], "source_url": "https://reactome.org/content/detail/R-HSA-2160492", "summary": "IL4I1:FAD oxidises L-Phe to kPPV is a Reactome release 97 curated reaction event. The source record connects L-Phe, H2O, O2 to kPPV, H2O2, NH4+ in extracellular region and cross-references RHEA:61241.", "equation": "L-Phe + H2O + O2 → kPPV + H2O2 + NH4+", "inputs": [ { "node_id": "R-ALL-351993", "reactome_id": "R-ALL-351993", "label": "L-Phe", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58095", "formula": "C9H11NO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-352327", "reactome_id": "R-ALL-352327", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2160466", "reactome_id": "R-ALL-2160466", "label": "kPPV", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18005", "formula": "C9H7O3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351629", "reactome_id": "R-ALL-351629", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-140912", "reactome_id": "R-ALL-140912", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-amino-acid oxidase activity of IL4I1:FAD [extracellular region]", "activity_id": "2160498", "ec_numbers": [ "1.4.3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:17356132", "title": "Human IL4I1 is a secreted L-phenylalanine oxidase expressed by mature dendritic cells that inhibits T-lymphocyte proliferation", "journal": "Blood", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17356132/" } ], "x": 6521.333333333333, "y": 1787.45, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2160851", "label": "HAS1,2,3 mediate the polymerization and export of HA", "schema_class": "Polymerisation", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:12529", "RHEA:20466" ], "source_url": "https://reactome.org/content/detail/R-HSA-2160851", "summary": "HAS1,2,3 mediate the polymerization and export of HA is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects UDP-GlcA, UDP-GlcNAc to H+, UDP, HA in plasma membrane · cytosol and cross-references RHEA:12529, RHEA:20466.", "equation": "UDP-GlcA + UDP-GlcNAc → 2 H+ + 2 UDP + HA", "inputs": [ { "node_id": "R-ALL-158601", "reactome_id": "R-ALL-158601", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-162756", "reactome_id": "R-ALL-162756", "label": "UDP-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-110096", "reactome_id": "R-ALL-110096", "label": "UDP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false } ], "catalysts": [ { "label": "hyaluronan synthase activity of HAS1,2,3:Mg2+ [plasma membrane]", "activity_id": "2142876", "ec_numbers": [ "2.4.1.212" ], "accessions": [] } ], "citations": [ { "id": "PMID:9083017", "title": "Molecular cloning and characterization of a cDNA encoding the third putative mammalian hyaluronan synthase", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9083017/" }, { "id": "PMID:8798544", "title": "Functional cloning of the cDNA for a human hyaluronan synthase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8798544/" }, { "id": "PMID:8798477", "title": "Molecular identification of a putative human hyaluronan synthase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8798477/" }, { "id": "PMID:35355017", "title": "Structure, substrate recognition and initiation of hyaluronan synthase", "journal": "Nature", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35355017/" }, { "id": "PMID:37769351", "title": "Hyaluronan synthases; mechanisms, myths, \u0026 mysteries of three types of unique bifunctional glycosyltransferases", "journal": "Glycobiology", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37769351/" }, { "id": "PMID:22343360", "title": "The hyaluronan synthase catalyzes the synthesis and membrane translocation of hyaluronan", "journal": "J Mol Biol", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22343360/" } ], "x": 2660, "y": 771.469696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-2160874", "label": "HYAL1 hydrolyses (HA)50", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2160874", "summary": "HYAL1 hydrolyses (HA)50 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects (HA)50, H2O to (HA)2 in lysosomal lumen.", "equation": "(HA)50 + 25 H2O → 25 (HA)2", "inputs": [ { "node_id": "R-ALL-2160864", "reactome_id": "R-ALL-2160864", "label": "(HA)50", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Polymer", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 25, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2160866", "reactome_id": "R-ALL-2160866", "label": "(HA)2", "role": "output", "stoichiometry": 25, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Polymer", "currency": false } ], "catalysts": [ { "label": "hyalurononglucosaminidase activity of HYAL1-like proteins [lysosomal lumen]", "activity_id": "1793220", "ec_numbers": [ "3.2.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:16104017", "title": "Structures of vertebrate hyaluronidases and their unique enzymatic mechanism of hydrolysis", "journal": "Proteins", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16104017/" } ], "x": 2381.3333333333335, "y": 1077.9545454545455, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-2160884", "label": "HA:HAR:HYAL2 binds to SLC9A1:p-CHP:Ca2+", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2160884", "summary": "HA:HAR:HYAL2 binds to SLC9A1:p-CHP:Ca2+ is a Reactome release 97 curated reaction event. The source record connects HA:HAR:HYAL2, SLC9A1:p-CHP:Ca2+ to HA:HAR:HYAL2:SLC9A1:pCHP:Ca2+ in plasma membrane.", "equation": "HA:HAR:HYAL2 + SLC9A1:p-CHP:Ca2+ → HA:HAR:HYAL2:SLC9A1:pCHP:Ca2+", "inputs": [ { "node_id": "R-HSA-2160889", "reactome_id": "R-HSA-2160889", "label": "HA:HAR:HYAL2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-5333682", "reactome_id": "R-HSA-5333682", "label": "SLC9A1:p-CHP:Ca2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2160891", "reactome_id": "R-HSA-2160891", "label": "HA:HAR:HYAL2:SLC9A1:pCHP:Ca2+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15090545", "title": "CD44 interaction with Na+-H+ exchanger (NHE1) creates acidic microenvironments leading to hyaluronidase-2 and cathepsin B activation and breast tumor cell invasion", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15090545/" }, { "id": "PMID:8901634", "title": "A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8901634/" }, { "id": "PMID:15035633", "title": "Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15035633/" }, { "id": "PMID:17050540", "title": "Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17050540/" }, { "id": "PMID:11350981", "title": "Calcineurin homologous protein as an essential cofactor for Na+/H+ exchangers", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11350981/" } ], "x": 2381.3333333333335, "y": 1054.378787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2160892", "label": "HYAL2 hydrolyses HA into 20kDa fragments", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2160892", "summary": "HYAL2 hydrolyses HA into 20kDa fragments is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects HA:HAR:HYAL2, H2O to (HA)50, HAR:HYAL2 in plasma membrane.", "equation": "HA:HAR:HYAL2 + H2O → (HA)50 + HAR:HYAL2", "inputs": [ { "node_id": "R-HSA-2160889", "reactome_id": "R-HSA-2160889", "label": "HA:HAR:HYAL2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2160894", "reactome_id": "R-ALL-2160894", "label": "(HA)50", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Polymer", "currency": false }, { "node_id": "R-HSA-9954181", "reactome_id": "R-HSA-9954181", "label": "HAR:HYAL2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "hyalurononglucosaminidase activity of HA:HAR:HYAL2:SLC9A1:pCHP:Ca2+ [plasma membrane]", "activity_id": "2160850", "ec_numbers": [ "3.2.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:15090545", "title": "CD44 interaction with Na+-H+ exchanger (NHE1) creates acidic microenvironments leading to hyaluronidase-2 and cathepsin B activation and breast tumor cell invasion", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15090545/" }, { "id": "PMID:16104017", "title": "Structures of vertebrate hyaluronidases and their unique enzymatic mechanism of hydrolysis", "journal": "Proteins", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16104017/" }, { "id": "PMID:9712871", "title": "HYAL2, a human gene expressed in many cells, encodes a lysosomal hyaluronidase with a novel type of specificity", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9712871/" }, { "id": "PMID:8901634", "title": "A calcineurin homologous protein inhibits GTPase-stimulated Na-H exchange", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8901634/" }, { "id": "PMID:17050540", "title": "Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17050540/" }, { "id": "PMID:15035633", "title": "Role of calcineurin B homologous protein in pH regulation by the Na+/H+ exchanger 1: tightly bound Ca2+ ions as important structural elements", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15035633/" } ], "x": 2660, "y": 1077.9545454545455, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2160906", "label": "20kDa HA fragments are translocated to lysosomes", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen · plasma membrane", "compartment_ids": [ "GO:0043202", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2160906", "summary": "20kDa HA fragments are translocated to lysosomes is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects (HA)50 to (HA)50 in lysosomal lumen · plasma membrane.", "equation": "(HA)50 → (HA)50", "inputs": [ { "node_id": "R-ALL-2160894", "reactome_id": "R-ALL-2160894", "label": "(HA)50", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Polymer", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2160864", "reactome_id": "R-ALL-2160864", "label": "(HA)50", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Polymer", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:22216413", "title": "Chain gangs: new aspects of hyaluronan metabolism", "journal": "Biochem Res Int", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22216413/" }, { "id": "PMID:11827788", "title": "CD44-mediated uptake and degradation of hyaluronan", "journal": "Matrix Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11827788/" } ], "x": 2938.666666666667, "y": 1113.3181818181818, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2160915", "label": "Receptor-mediated uptake of HA", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2160915", "summary": "Receptor-mediated uptake of HA is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects HA, HYAL2, HARs to HA:HAR:HYAL2 in plasma membrane · extracellular region.", "equation": "HA + HYAL2 + HARs → HA:HAR:HYAL2", "inputs": [ { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false }, { "node_id": "R-HSA-2160919", "reactome_id": "R-HSA-2160919", "label": "HYAL2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2160926", "reactome_id": "R-HSA-2160926", "label": "HARs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2160889", "reactome_id": "R-HSA-2160889", "label": "HA:HAR:HYAL2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of HARs [plasma membrane]", "activity_id": "9954862", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:1703543", "title": "The hyaluronate receptor is a member of the CD44 (H-CAM) family of cell surface glycoproteins", "journal": "J Cell Biol", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/1703543/" }, { "id": "PMID:1426053", "title": "Hyaluronate binding and CD44 expression in human glioblastoma cells and astrocytes", "journal": "Exp Cell Res", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1426053/" }, { "id": "PMID:9601098", "title": "The human hyaluronan receptor RHAMM is expressed as an intracellular protein in breast cancer cells", "journal": "J Cell Sci", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9601098/" }, { "id": "PMID:8890751", "title": "The characterization of a human RHAMM cDNA: conservation of the hyaluronan-binding domains", "journal": "Gene", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8890751/" }, { "id": "PMID:17145755", "title": "Expression, processing, and glycosaminoglycan binding activity of the recombinant human 315-kDa hyaluronic acid receptor for endocytosis (HARE)", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17145755/" }, { "id": "PMID:18434317", "title": "The human hyaluronan receptor for endocytosis (HARE/Stabilin-2) is a systemic clearance receptor for heparin", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18434317/" } ], "x": 2660, "y": 1042.590909090909, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-2161187", "label": "MAPDA hydrolyzes N6-methyl-AMP to IMP and methylamine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16002" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161187", "summary": "MAPDA hydrolyzes N6-methyl-AMP to IMP and methylamine is a Reactome release 97 curated reaction event. The source record connects H2O, N(6)-methyl-AMP(2-), H+ to methylamine, IMP in cytosol and cross-references RHEA:16002.", "equation": "H2O + N(6)-methyl-AMP(2-) + H+ → methylamine + IMP", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731626", "reactome_id": "R-ALL-9731626", "label": "N(6)-methyl-AMP(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:144842", "formula": "C11H14N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9731652", "reactome_id": "R-ALL-9731652", "label": "methylamine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59338", "formula": "CH6N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29602", "reactome_id": "R-ALL-29602", "label": "IMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58053", "formula": "C10H11N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N6-methyl-AMP deaminase activity of MAPDA:Zn [cytosol]", "activity_id": "2161186", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:21755941", "title": "Adenosine deaminase-like protein 1 (ADAL1): characterization and substrate specificity in the hydrolysis of N(6)- or O(6)-substituted purine or 2-aminopurine nucleoside monophosphates", "journal": "J Med Chem", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21755941/" }, { "id": "PMID:32144205", "title": "MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool", "journal": "J Biol Chem", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32144205/" } ], "x": 3321.3333333333335, "y": 2941.8214285714284, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161549", "label": "PGH2 is reduced to PGF2a by AKR1C3", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:45314" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161549", "summary": "PGH2 is reduced to PGF2a by AKR1C3 is a Reactome release 97 curated reaction event. The source record connects PGH2, NADPH, H+ to PGF2a, NADP+ in cytosol and cross-references RHEA:45314.", "equation": "PGH2 + NADPH + H+ → PGF2a + NADP+", "inputs": [ { "node_id": "R-ALL-265283", "reactome_id": "R-ALL-265283", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-879535", "reactome_id": "R-ALL-879535", "label": "PGF2a", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57404", "formula": "C20H33O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "prostaglandin H2 endoperoxidase reductase activity of AKR1C3 [cytosol]", "activity_id": "2161558", "ec_numbers": null, "accessions": [ "P42330" ] } ], "citations": [ { "id": "PMID:10622721", "title": "cDNA cloning, expression and characterization of human prostaglandin F synthase", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10622721/" }, { "id": "PMID:14979715", "title": "Crystal structure of human prostaglandin F synthase (AKR1C3)", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14979715/" }, { "id": "PMID:16475787", "title": "Prostaglandin F2alpha formation from prostaglandin H2 by prostaglandin F synthase (PGFS): crystal structure of PGFS containing bimatoprost", "journal": "Biochemistry", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16475787/" } ], "x": 311.33333333333337, "y": 1546.578947368421, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161563", "label": "PGJ2 is isomerised to delta12-PGJ2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [ "RHEA:53549" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161563", "summary": "PGJ2 is isomerised to delta12-PGJ2 is a Reactome release 97 curated reaction event. The source record connects PGJ2 to delta12-PGJ2 in endoplasmic reticulum lumen and cross-references RHEA:53549.", "equation": "PGJ2 → delta12-PGJ2", "inputs": [ { "node_id": "R-ALL-2142674", "reactome_id": "R-ALL-2142674", "label": "PGJ2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133396", "formula": "C20H29O4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142821", "reactome_id": "R-ALL-2142821", "label": "delta12-PGJ2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133424", "formula": "C20H29O4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "intramolecular oxidoreductase activity, transposing C=C bonds of oxidoreductase [cytosol]", "activity_id": "9959910", "ec_numbers": [ "5.3.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:11907120", "title": "15-Deoxy-delta 12,14-prostaglandins D2 and J2 are potent activators of human eosinophils", "journal": "J Immunol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11907120/" } ], "x": 868.6666666666667, "y": 1625.0350877192982, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161567", "label": "LTB4 is oxidised to 12-oxoLTB4 by PTGR1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50609" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161567", "summary": "LTB4 is oxidised to 12-oxoLTB4 by PTGR1 is a Reactome release 97 curated reaction event. The source record connects LTB4, NADP+ to 12-oxoLTB4, NADPH, H+ in cytosol and cross-references RHEA:50609.", "equation": "LTB4 + NADP+ → 12-oxoLTB4 + NADPH + H+", "inputs": [ { "node_id": "R-ALL-266056", "reactome_id": "R-ALL-266056", "label": "LTB4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57461", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142810", "reactome_id": "R-ALL-2142810", "label": "12-oxoLTB4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133309", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "leukotriene B4 12-hydroxy dehydrogenase activity of PTGR1 [cytosol]", "activity_id": "2161632", "ec_numbers": null, "accessions": [ "Q14914" ] } ], "citations": [ { "id": "PMID:8576264", "title": "cDNA cloning, expression, and mutagenesis study of leukotriene B4 12-hydroxydehydrogenase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8576264/" }, { "id": "PMID:8394361", "title": "Enzymatic inactivation of leukotriene B4 by a novel enzyme found in the porcine kidney. Purification and properties of leukotriene B4 12-hydroxydehydrogenase", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8394361/" }, { "id": "PMID:9461497", "title": "Purification, cDNA cloning and expression of 15-oxoprostaglandin 13-reductase from pig lung", "journal": "Biochem J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9461497/" } ], "x": 590, "y": 1546.578947368421, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161588", "label": "Delta12-PGJ2 is dehydrated to 15d-PGJ2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161588", "summary": "Delta12-PGJ2 is dehydrated to 15d-PGJ2 is a Reactome release 97 curated reaction event. The source record connects delta12-PGJ2 to 15d-PGJ2, H2O in endoplasmic reticulum lumen.", "equation": "delta12-PGJ2 → 15d-PGJ2 + H2O", "inputs": [ { "node_id": "R-ALL-2142821", "reactome_id": "R-ALL-2142821", "label": "delta12-PGJ2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133424", "formula": "C20H29O4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142702", "reactome_id": "R-ALL-2142702", "label": "15d-PGJ2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:85236", "formula": "C20H27O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:11907120", "title": "15-Deoxy-delta 12,14-prostaglandins D2 and J2 are potent activators of human eosinophils", "journal": "J Immunol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11907120/" } ], "x": 311.33333333333337, "y": 1736.6842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161612", "label": "PGH2 is reduced to PGF2a by FAM213B", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:28215" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161612", "summary": "PGH2 is reduced to PGF2a by FAM213B is a Reactome release 97 curated reaction event. The source record connects PGH2, TXN-S2H2 to PGF2a, TXN-S2 in cytosol and cross-references RHEA:28215.", "equation": "PGH2 + TXN-S2H2 → PGF2a + TXN-S2", "inputs": [ { "node_id": "R-ALL-265283", "reactome_id": "R-ALL-265283", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2142774", "reactome_id": "R-ALL-2142774", "label": "TXN-S2H2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15967", "formula": "C6H9NO2S2R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-879535", "reactome_id": "R-ALL-879535", "label": "PGF2a", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57404", "formula": "C20H33O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2142833", "reactome_id": "R-ALL-2142833", "label": "TXN-S2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18191", "formula": "C6H8N2O2S2R4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of FAM213B [cytosol]", "activity_id": "2161731", "ec_numbers": [ "1" ], "accessions": [ "Q8TBF2" ] } ], "citations": [ { "id": "PMID:18006499", "title": "Molecular characterization of a novel type of prostamide/prostaglandin F synthase, belonging to the thioredoxin-like superfamily", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18006499/" }, { "id": "PMID:20950588", "title": "Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system", "journal": "Brain Res", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/20950588/" } ], "x": 590, "y": 1679.3508771929824, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161613", "label": "PGH2 is degraded to 12S-HHT and MDA by TBXAS1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:48645" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161613", "summary": "PGH2 is degraded to 12S-HHT and MDA by TBXAS1 is a Reactome release 97 curated reaction event. The source record connects PGH2 to 12S-HHT, MDA in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:48645.", "equation": "PGH2 → 12S-HHT + MDA", "inputs": [ { "node_id": "R-ALL-30138", "reactome_id": "R-ALL-30138", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142797", "reactome_id": "R-ALL-2142797", "label": "12S-HHT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90694", "formula": "C17H27O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2161562", "reactome_id": "R-ALL-2161562", "label": "MDA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:566274", "formula": "C3H4O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "12-hydroxyheptadecatrienoic acid synthase activity of TBXAS1 [endoplasmic reticulum membrane]", "activity_id": "2161594", "ec_numbers": null, "accessions": [ "P24557" ] } ], "citations": [ { "id": "PMID:11297515", "title": "Substrate binding is the rate-limiting step in thromboxane synthase catalysis", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11297515/" } ], "x": 590, "y": 1597.877192982456, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161614", "label": "PGD2 is reduced to 11-epi-PGF2a by AKRIC3", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:45317" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161614", "summary": "PGD2 is reduced to 11-epi-PGF2a by AKRIC3 is a Reactome release 97 curated reaction event. The source record connects PGD2, H+, NADPH to 11epi-PGF2a, NADP+ in cytosol and cross-references RHEA:45317.", "equation": "PGD2 + H+ + NADPH → 11epi-PGF2a + NADP+", "inputs": [ { "node_id": "R-ALL-879629", "reactome_id": "R-ALL-879629", "label": "PGD2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57406", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142710", "reactome_id": "R-ALL-2142710", "label": "11epi-PGF2a", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:85173", "formula": "C20H33O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "prostaglandin D2 11-ketoreductase activity of AKR1C3 [cytosol]", "activity_id": "2161608", "ec_numbers": [ "1.1.1.188" ], "accessions": [ "P42330" ] } ], "citations": [ { "id": "PMID:3862115", "title": "Transformation of prostaglandin D2 to 9 alpha, 11 beta-(15S)-trihydroxyprosta-(5Z,13E)-dien-1-oic acid (9 alpha, 11 beta-prostaglandin F2): a unique biologically active prostaglandin produced enzymatically in vivo in humans", "journal": "Proc Natl Acad Sci U S A", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3862115/" }, { "id": "PMID:15047184", "title": "Synthesis of prostaglandin F ethanolamide by prostaglandin F synthase and identification of Bimatoprost as a potent inhibitor of the enzyme: new enzyme assay method using LC/ESI/MS", "journal": "Arch Biochem Biophys", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15047184/" }, { "id": "PMID:20036328", "title": "Aldo-keto reductase 1C3 expression in MCF-7 cells reveals roles in steroid hormone and prostaglandin metabolism that may explain its over-expression in breast cancer", "journal": "J Steroid Biochem Mol Biol", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20036328/" } ], "x": 868.6666666666667, "y": 1546.578947368421, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161619", "label": "PGI2 is hydrolysed to 6k-PGF1a", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161619", "summary": "PGI2 is hydrolysed to 6k-PGF1a is a Reactome release 97 curated reaction event. The source record connects PGI2, H2O to 6k-PGF1a in endoplasmic reticulum lumen.", "equation": "PGI2 + H2O → 6k-PGF1a", "inputs": [ { "node_id": "R-ALL-31593", "reactome_id": "R-ALL-31593", "label": "PGI2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57403", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142786", "reactome_id": "R-ALL-2142786", "label": "6k-PGF1a", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133451", "formula": "C20H33O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15115769", "title": "Purification and characterization of recombinant human prostacyclin synthase", "journal": "J Biochem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15115769/" } ], "x": 590, "y": 1736.6842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161620", "label": "PGH2 is isomerised to PGD2 by PTGDS", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:10601" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161620", "summary": "PGH2 is isomerised to PGD2 by PTGDS is a Reactome release 97 curated reaction event. The source record connects PGH2 to PGD2 in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:10601.", "equation": "PGH2 → PGD2", "inputs": [ { "node_id": "R-ALL-30138", "reactome_id": "R-ALL-30138", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2161634", "reactome_id": "R-ALL-2161634", "label": "PGD2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57406", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "prostaglandin-D synthase activity of PTGDS [endoplasmic reticulum membrane]", "activity_id": "2161647", "ec_numbers": [ "5.3.99.2" ], "accessions": [ "P41222" ] } ], "citations": [ { "id": "PMID:20667974", "title": "Structure-function analysis of human l-prostaglandin D synthase bound with fatty acid molecules", "journal": "FASEB J", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20667974/" } ], "x": 311.33333333333337, "y": 1628.0526315789473, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161651", "label": "PGE2 is converted to PGF2a by CBR1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24510" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161651", "summary": "PGE2 is converted to PGF2a by CBR1 is a Reactome release 97 curated reaction event. The source record connects PGE2, NADPH, H+ to PGF2a, NADP+ in cytosol and cross-references RHEA:24510.", "equation": "PGE2 + NADPH + H+ → PGF2a + NADP+", "inputs": [ { "node_id": "R-ALL-265287", "reactome_id": "R-ALL-265287", "label": "PGE2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:606564", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-879535", "reactome_id": "R-ALL-879535", "label": "PGF2a", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57404", "formula": "C20H33O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "prostaglandin E2 9-reductase activity of CBR1 [cytosol]", "activity_id": "2161655", "ec_numbers": [ "1.1.1.189" ], "accessions": [ "P16152" ] } ], "citations": [ { "id": "PMID:7005231", "title": "Purification and properties of an NADPH-dependent carbonyl reductase from human brain. Relationship to prostaglandin 9-ketoreductase and xenobiotic ketone reductase", "journal": "J Biol Chem", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/7005231/" }, { "id": "PMID:18493841", "title": "Different functions between human monomeric carbonyl reductase 3 and carbonyl reductase 1", "journal": "Mol Cell Biochem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18493841/" } ], "x": 311.33333333333337, "y": 1549.5964912280701, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161659", "label": "PGE2 is dehydrated to PGA2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50537" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161659", "summary": "PGE2 is dehydrated to PGA2 is a Reactome release 97 curated reaction event. The source record connects PGE2 to H2O, PGA2 in cytosol and cross-references RHEA:50537.", "equation": "PGE2 → H2O + PGA2", "inputs": [ { "node_id": "R-ALL-265287", "reactome_id": "R-ALL-265287", "label": "PGE2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:606564", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2299726", "reactome_id": "R-ALL-2299726", "label": "PGA2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133370", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydro-lyase activity of hydro-lyase [cytosol]", "activity_id": "9915657", "ec_numbers": [ "4.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:5903721", "title": "Prostaglandins in human seminal plasma. Prostaglandins and related factors 46", "journal": "J Biol Chem", "year": 1966, "url": "https://pubmed.ncbi.nlm.nih.gov/5903721/" }, { "id": "PMID:2717650", "title": "Simultaneous determination of prostaglandins (PG) E2, A2 and B2 and stability studies of PGE2 in pharmaceutical preparations by ion-pair reversed phase HPLC", "journal": "Pharm Acta Helv", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2717650/" } ], "x": 868.6666666666667, "y": 1597.877192982456, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161660", "label": "PGH2 is isomerised to PGE2 by PTGES", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:12894" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161660", "summary": "PGH2 is isomerised to PGE2 by PTGES is a Reactome release 97 curated reaction event. The source record connects PGH2 to PGE2 in endoplasmic reticulum membrane · cytosol and cross-references RHEA:12894.", "equation": "PGH2 → PGE2", "inputs": [ { "node_id": "R-ALL-265283", "reactome_id": "R-ALL-265283", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-265287", "reactome_id": "R-ALL-265287", "label": "PGE2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:606564", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "prostaglandin-E synthase activity of PTGES trimer [endoplasmic reticulum membrane]", "activity_id": "2161577", "ec_numbers": [ "5.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:18682561", "title": "Structural basis for induced formation of the inflammatory mediator prostaglandin E2", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18682561/" } ], "x": 590, "y": 1628.0526315789473, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161662", "label": "PGD2/E2/F2a is oxidised to 15k-PGD2/E2/F2a by HPGD", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161662", "summary": "PGD2/E2/F2a is oxidised to 15k-PGD2/E2/F2a by HPGD is a Reactome release 97 curated reaction event. The source record connects PGD2/E2/F2a, NAD+ to 15k-PGD2,15k-PGE2,15k-PGF2a, NADH, H+ in cytosol.", "equation": "PGD2/E2/F2a + NAD+ → 15k-PGD2,15k-PGE2,15k-PGF2a + NADH + H+", "inputs": [ { "node_id": "R-ALL-2161661", "reactome_id": "R-ALL-2161661", "label": "PGD2/E2/F2a", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2161597", "reactome_id": "R-ALL-2161597", "label": "15k-PGD2,15k-PGE2,15k-PGF2a", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "15-hydroxyprostaglandin dehydrogenase (NAD+) activity of HPGD dimer [cytosol]", "activity_id": "2161623", "ec_numbers": [ "1.1.1.141" ], "accessions": [] } ], "citations": [ { "id": "PMID:16828555", "title": "Role of glutamine 148 of human 15-hydroxyprostaglandin dehydrogenase in catalytic oxidation of prostaglandin E2", "journal": "Bioorg Med Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16828555/" }, { "id": "PMID:3954355", "title": "Isolation and properties of lung 15-hydroxyprostaglandin dehydrogenase from pregnant rabbits", "journal": "Arch Biochem Biophys", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3954355/" } ], "x": 868.6666666666667, "y": 1718.578947368421, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161666", "label": "PGA2 is isomerised to PGC2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50541" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161666", "summary": "PGA2 is isomerised to PGC2 is a Reactome release 97 curated reaction event. The source record connects PGA2 to PGC2 in cytosol and cross-references RHEA:50541.", "equation": "PGA2 → PGC2", "inputs": [ { "node_id": "R-ALL-2299726", "reactome_id": "R-ALL-2299726", "label": "PGA2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133370", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2299724", "reactome_id": "R-ALL-2299724", "label": "PGC2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133392", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "isomerase activity of isomerase [cytosol]", "activity_id": "9915637", "ec_numbers": [ "5" ], "accessions": [] } ], "citations": [ { "id": "PMID:11301410", "title": "Cyclopentenone prostaglandins: new insights on biological activities and cellular targets", "journal": "Med Res Rev", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11301410/" } ], "x": 868.6666666666667, "y": 1628.0526315789473, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161668", "label": "PGA2 is dehydrated to 15d-PGA2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161668", "summary": "PGA2 is dehydrated to 15d-PGA2 is a Reactome release 97 curated reaction event. The source record connects PGA2 to 15d-PGA2, H2O in cytosol.", "equation": "PGA2 → 15d-PGA2 + H2O", "inputs": [ { "node_id": "R-ALL-2299726", "reactome_id": "R-ALL-2299726", "label": "PGA2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133370", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2299722", "reactome_id": "R-ALL-2299722", "label": "15d-PGA2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63975", "formula": "C20H28O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:10200320", "title": "Cyclopentenone prostaglandins suppress activation of microglia: down-regulation of inducible nitric-oxide synthase by 15-deoxy-Delta12,14-prostaglandin J2", "journal": "Proc Natl Acad Sci U S A", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10200320/" } ], "x": 868.6666666666667, "y": 1736.6842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161673", "label": "PGD2 is dehydrated to 15d-PGD2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161673", "summary": "PGD2 is dehydrated to 15d-PGD2 is a Reactome release 97 curated reaction event. The source record connects PGD2 to 15d-PGD2, H2O in endoplasmic reticulum lumen.", "equation": "PGD2 → 15d-PGD2 + H2O", "inputs": [ { "node_id": "R-ALL-2161634", "reactome_id": "R-ALL-2161634", "label": "PGD2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57406", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142817", "reactome_id": "R-ALL-2142817", "label": "15d-PGD2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63999", "formula": "C20H30O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:11907120", "title": "15-Deoxy-delta 12,14-prostaglandins D2 and J2 are potent activators of human eosinophils", "journal": "J Immunol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11907120/" } ], "x": 311.33333333333337, "y": 1739.701754385965, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161692", "label": "15k-PGE2/F2a is reduced to dhk-PGE2/F2a by PTGR1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11914", "RHEA:50590" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161692", "summary": "15k-PGE2/F2a is reduced to dhk-PGE2/F2a by PTGR1 is a Reactome release 97 curated reaction event. The source record connects 15k-PGE2,15k-PGF2a, NADPH, H+ to dhk-PGE2,dhk-PGF2a, NADP+ in cytosol and cross-references RHEA:11914, RHEA:50590.", "equation": "15k-PGE2,15k-PGF2a + NADPH + H+ → dhk-PGE2,dhk-PGF2a + NADP+", "inputs": [ { "node_id": "R-ALL-2161650", "reactome_id": "R-ALL-2161650", "label": "15k-PGE2,15k-PGF2a", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2161684", "reactome_id": "R-ALL-2161684", "label": "dhk-PGE2,dhk-PGF2a", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "15-oxoprostaglandin 13-reductase [NAD(P)+] activity of PTGR2 [cytosol]", "activity_id": "2161566", "ec_numbers": [ "1.3.1.48" ], "accessions": [ "Q8N8N7" ] } ], "citations": [ { "id": "PMID:17449869", "title": "Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of peroxisome proliferator-activated receptor gamma activation", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17449869/" }, { "id": "PMID:19000823", "title": "Structural basis for catalytic and inhibitory mechanisms of human prostaglandin reductase PTGR2", "journal": "Structure", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/19000823/" } ], "x": 311.33333333333337, "y": 1600.8947368421052, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161701", "label": "PGH2 is isomerised to PGD2 by HPGDS", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10601" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161701", "summary": "PGH2 is isomerised to PGD2 by HPGDS is a Reactome release 97 curated reaction event. The source record connects PGH2 to PGD2 in cytosol and cross-references RHEA:10601.", "equation": "PGH2 → PGD2", "inputs": [ { "node_id": "R-ALL-265283", "reactome_id": "R-ALL-265283", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-879629", "reactome_id": "R-ALL-879629", "label": "PGD2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57406", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "prostaglandin-D synthase activity of HPGDS dimer [cytosol]", "activity_id": "2161729", "ec_numbers": [ "5.3.99.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11672424", "title": "Mammalian class Sigma glutathione S-transferases: catalytic properties and tissue-specific expression of human and rat GSH-dependent prostaglandin D2 synthases", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11672424/" }, { "id": "PMID:12627223", "title": "Mechanism of metal activation of human hematopoietic prostaglandin D synthase", "journal": "Nat Struct Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12627223/" } ], "x": 311.33333333333337, "y": 1631.0701754385964, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161732", "label": "TXB2 is converted to 11dh-TXB2 by TXDH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [ "RHEA:52313" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161732", "summary": "TXB2 is converted to 11dh-TXB2 by TXDH is a Reactome release 97 curated reaction event. The source record connects TXB2, NAD+ to 11dh-TXB2, NADH, H+ in endoplasmic reticulum lumen and cross-references RHEA:52313.", "equation": "TXB2 + NAD+ → 11dh-TXB2 + NADH + H+", "inputs": [ { "node_id": "R-ALL-443890", "reactome_id": "R-ALL-443890", "label": "TXB2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90696", "formula": "C20H33O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194653", "reactome_id": "R-ALL-194653", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142828", "reactome_id": "R-ALL-2142828", "label": "11dh-TXB2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:136539", "formula": "C20H31O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194697", "reactome_id": "R-ALL-194697", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "11-hydroxythromboxane B2 dehydrogenase activity of TXDH [endoplasmic reticulum lumen]", "activity_id": "2161726", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:3823488", "title": "Radioimmunoassay for 11-dehydro-TXB2: a method for monitoring thromboxane production in vivo", "journal": "Prostaglandins", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3823488/" }, { "id": "PMID:3461463", "title": "11-Dehydrothromboxane B2: a quantitative index of thromboxane A2 formation in the human circulation", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3461463/" }, { "id": "PMID:8200461", "title": "11-Hydroxythromboxane B2 dehydrogenase is identical to cytosolic aldehyde dehydrogenase", "journal": "FEBS Lett", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8200461/" } ], "x": 590, "y": 1549.5964912280701, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161733", "label": "PGD2 is dehydrated to PGJ2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [ "RHEA:50557" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161733", "summary": "PGD2 is dehydrated to PGJ2 is a Reactome release 97 curated reaction event. The source record connects PGD2 to PGJ2, H2O in endoplasmic reticulum lumen and cross-references RHEA:50557.", "equation": "PGD2 → PGJ2 + H2O", "inputs": [ { "node_id": "R-ALL-2161634", "reactome_id": "R-ALL-2161634", "label": "PGD2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57406", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142674", "reactome_id": "R-ALL-2142674", "label": "PGJ2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133396", "formula": "C20H29O4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:11907120", "title": "15-Deoxy-delta 12,14-prostaglandins D2 and J2 are potent activators of human eosinophils", "journal": "J Immunol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11907120/" } ], "x": 311.33333333333337, "y": 1640.122807017544, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161735", "label": "PGC2 is isomerised to PGB2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50545" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161735", "summary": "PGC2 is isomerised to PGB2 is a Reactome release 97 curated reaction event. The source record connects PGC2 to PGB2 in cytosol and cross-references RHEA:50545.", "equation": "PGC2 → PGB2", "inputs": [ { "node_id": "R-ALL-2299724", "reactome_id": "R-ALL-2299724", "label": "PGC2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133392", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2299721", "reactome_id": "R-ALL-2299721", "label": "PGB2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133391", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "isomerase activity of isomerase [cytosol]", "activity_id": "9915637", "ec_numbers": [ "5" ], "accessions": [] } ], "citations": [ { "id": "PMID:11301410", "title": "Cyclopentenone prostaglandins: new insights on biological activities and cellular targets", "journal": "Med Res Rev", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11301410/" } ], "x": 590, "y": 1631.0701754385964, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161745", "label": "20oh-LTB4 is oxidised to 20cho-LTB4 by CYP4F2/4F3", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:48669" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161745", "summary": "20oh-LTB4 is oxidised to 20cho-LTB4 by CYP4F2/4F3 is a Reactome release 97 curated reaction event. The source record connects 20OH-LTB4, NADPH, H+ to 20cho-LTB4, NADP+, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:48669.", "equation": "20OH-LTB4 + NADPH + H+ + O2 → 20cho-LTB4 + NADP+ + 2 H2O", "inputs": [ { "node_id": "R-ALL-2161636", "reactome_id": "R-ALL-2161636", "label": "20OH-LTB4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57460", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142740", "reactome_id": "R-ALL-2142740", "label": "20cho-LTB4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90720", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "20-hydroxy-leukotriene B4 omega oxidase activity of Cytochrome P450 (CYP4F2/4F3 based) [endoplasmic reticulum membrane]", "activity_id": "2161740", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:2836406", "title": "The identification and formation of 20-aldehyde leukotriene B4", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2836406/" } ], "x": 311.33333333333337, "y": 1646.157894736842, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161768", "label": "EXA4 is converted to EXC4 by LTC4S", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · nuclear envelope", "compartment_ids": [ "GO:0005829", "GO:0005635" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161768", "summary": "EXA4 is converted to EXC4 by LTC4S is a Reactome release 97 curated reaction event. The source record connects EXA4, GSH to EXC4 in cytosol · nuclear envelope.", "equation": "EXA4 + GSH → EXC4", "inputs": [ { "node_id": "R-ALL-2142772", "reactome_id": "R-ALL-2142772", "label": "EXA4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132070", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142726", "reactome_id": "R-ALL-2142726", "label": "EXC4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63984", "formula": "C30H47N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "eoxin C4 synthase activity of ALOX5:ALOX5AP:LTC4S [nuclear envelope]", "activity_id": "2161978", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18184802", "title": "Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and mast cells", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18184802/" }, { "id": "PMID:18647347", "title": "Hodgkin Reed-Sternberg cells express 15-lipoxygenase-1 and are putative producers of eoxins in vivo: novel insight into the inflammatory features of classical Hodgkin lymphoma", "journal": "FEBS J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18647347/" } ], "x": 311.33333333333337, "y": 1721.5964912280701, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161775", "label": "ALOX12 oxidises LTA4 to LXA4", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:48585" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161775", "summary": "ALOX12 oxidises LTA4 to LXA4 is a Reactome release 97 curated reaction event. The source record connects LTA4, O2, H2O to LXA4 in cytosol and cross-references RHEA:48585.", "equation": "2 LTA4 + O2 + 2 H2O → 2 LXA4", "inputs": [ { "node_id": "R-ALL-9032038", "reactome_id": "R-ALL-9032038", "label": "LTA4", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2161759", "reactome_id": "R-ALL-2161759", "label": "LXA4", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:67026", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen of ALOX12:Fe2+ [cytosol]", "activity_id": "2161800", "ec_numbers": [ "1.13.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:8250832", "title": "Lipoxin synthase activity of human platelet 12-lipoxygenase", "journal": "Biochem J", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8250832/" }, { "id": "PMID:2155925", "title": "Lipoxin formation during human neutrophil-platelet interactions. Evidence for the transformation of leukotriene A4 by platelet 12-lipoxygenase in vitro", "journal": "J Clin Invest", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2155925/" }, { "id": "PMID:3770188", "title": "Evidence for a 5(6)-epoxytetraene intermediate in the biosynthesis of lipoxins in human leukocytes. Conversion into lipoxin A by cytosolic epoxide hydrolase", "journal": "FEBS Lett", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3770188/" } ], "x": 5670, "y": 2035.154761904762, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161776", "label": "5S-HETE is oxidised to 5-oxoETE by 5-HEDH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161776", "summary": "5S-HETE is oxidised to 5-oxoETE by 5-HEDH is a Reactome release 97 curated reaction event. The source record connects 5S-HETE, NADP+ to 5-oxoETE, NADPH, H+ in cytosol.", "equation": "5S-HETE + NADP+ → 5-oxoETE + NADPH + H+", "inputs": [ { "node_id": "R-ALL-2142733", "reactome_id": "R-ALL-2142733", "label": "5S-HETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90632", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2161921", "reactome_id": "R-ALL-2161921", "label": "5-oxoETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65342", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "5(S)-hydroxyeicosatetraenoic acid dehydrogenase activity of 5-HEDH [cytosol]", "activity_id": "2161975", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:1326548", "title": "Metabolism of 5(S)-hydroxy-6,8,11,14-eicosatetraenoic acid and other 5(S)-hydroxyeicosanoids by a specific dehydrogenase in human polymorphonuclear leukocytes", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1326548/" } ], "x": 590, "y": 1709.5263157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161779", "label": "LXA4 is oxidised to 15k-LXA4 by HPGD", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:41573" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161779", "summary": "LXA4 is oxidised to 15k-LXA4 by HPGD is a Reactome release 97 curated reaction event. The source record connects LXA4, NAD+ to 15k-LXA4, NADH, H+ in cytosol and cross-references RHEA:41573.", "equation": "LXA4 + NAD+ → 15k-LXA4 + NADH + H+", "inputs": [ { "node_id": "R-ALL-2161759", "reactome_id": "R-ALL-2161759", "label": "LXA4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:67026", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142761", "reactome_id": "R-ALL-2142761", "label": "15k-LXA4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78311", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "15-hydroxyprostaglandin dehydrogenase (NAD+) activity of HPGD dimer [cytosol]", "activity_id": "2161623", "ec_numbers": [ "1.1.1.141" ], "accessions": [] } ], "citations": [ { "id": "PMID:10837478", "title": "Oxidoreductases in lipoxin A4 metabolic inactivation: a novel role for 15-oxoprostaglandin 13-reductase/leukotriene B4 12-hydroxydehydrogenase in inflammation", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10837478/" } ], "x": 5670, "y": 2016.6309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161789", "label": "15S-HETE is oxidised to 15-oxoETE by 15-HEDH", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23261", "RHEA:23265", "RHEA:23260", "RHEA:23264" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161789", "summary": "15S-HETE is oxidised to 15-oxoETE by 15-HEDH is a Reactome release 97 curated reaction event. The source record connects 15S-HETE, NAD(P)+ to 15-oxoETE, NAD(P)H, H+ in cytosol and cross-references RHEA:23261, RHEA:23265, RHEA:23260, RHEA:23264.", "equation": "15S-HETE + NAD(P)+ → 15-oxoETE + NAD(P)H + H+", "inputs": [ { "node_id": "R-ALL-2142707", "reactome_id": "R-ALL-2142707", "label": "15S-HETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57409", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428218", "reactome_id": "R-ALL-428218", "label": "NAD(P)+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142747", "reactome_id": "R-ALL-2142747", "label": "15-oxoETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57410", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428206", "reactome_id": "R-ALL-428206", "label": "NAD(P)H", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "15-hydroxyicosatetraenoate dehydrogenase activity of 15-HEDH [cytosol]", "activity_id": "2161741", "ec_numbers": [ "1.1.1.232" ], "accessions": [] } ], "citations": [ { "id": "PMID:17662651", "title": "Expression of 15-lipoxygenase type-1 in human mast cells", "journal": "Biochim Biophys Acta", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17662651/" } ], "x": 4451.333333333333, "y": 2206.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161790", "label": "20cooh-LTB4 is converted to 18cooh-LTB4", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161790", "summary": "20cooh-LTB4 is converted to 18cooh-LTB4 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 20cooh-LTB4 to 18cooh-LTB4 in cytosol.", "equation": "20cooh-LTB4 → 18cooh-LTB4", "inputs": [ { "node_id": "R-ALL-2161582", "reactome_id": "R-ALL-2161582", "label": "20cooh-LTB4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90722", "formula": "C20H28O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142676", "reactome_id": "R-ALL-2142676", "label": "18cooh-LTB4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63980", "formula": "C18H26O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12709426", "title": "Urinary metabolites of leukotriene B4 in the human subject", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12709426/" }, { "id": "PMID:9862787", "title": "Metabolic transformations of leukotriene B4 in primary cultures of human hepatocytes", "journal": "J Pharmacol Exp Ther", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9862787/" } ], "x": 590, "y": 1757.8070175438597, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-2161791", "label": "15S-HpETE is reduced to 15S-HETE by GPX1/2/4", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:76696" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161791", "summary": "15S-HpETE is reduced to 15S-HETE by GPX1/2/4 is a Reactome release 97 curated reaction event. The source record connects 15S-HpETE, GSH to 15S-HETE, GSSG, H2O in cytosol and cross-references RHEA:76696.", "equation": "15S-HpETE + 2 GSH → 15S-HETE + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-2142718", "reactome_id": "R-ALL-2142718", "label": "15S-HpETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57446", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142707", "reactome_id": "R-ALL-2142707", "label": "15S-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57409", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glutathione peroxidase activity of GPX1/2/4 [cytosol]", "activity_id": "2161937", "ec_numbers": [ "1.11.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:2501828", "title": "Role of glutathione and glutathione peroxidase in human platelet arachidonic acid metabolism", "journal": "Prostaglandins", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2501828/" } ], "x": 868.6666666666667, "y": 1549.5964912280701, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161792", "label": "20cho-LTB4 is oxidised to 20cooh-LTB4 by CYP4F2/4F3", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:48673" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161792", "summary": "20cho-LTB4 is oxidised to 20cooh-LTB4 by CYP4F2/4F3 is a Reactome release 97 curated reaction event. The source record connects 20cho-LTB4, NADPH, H+ to 20cooh-LTB4, NADP+, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:48673.", "equation": "20cho-LTB4 + NADPH + H+ + O2 → 20cooh-LTB4 + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-2142740", "reactome_id": "R-ALL-2142740", "label": "20cho-LTB4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90720", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2161582", "reactome_id": "R-ALL-2161582", "label": "20cooh-LTB4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90722", "formula": "C20H28O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "20-aldehyde-leukotriene B4 20-monooxygenase activity of Cytochrome P450 (CYP4F2/4F3 based) [endoplasmic reticulum membrane]", "activity_id": "2161602", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:2836406", "title": "The identification and formation of 20-aldehyde leukotriene B4", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2836406/" } ], "x": 590, "y": 1646.157894736842, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161794", "label": "Arachidonate is converted to HXA3/B3 by ALOX12", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161794", "summary": "Arachidonate is converted to HXA3/B3 by ALOX12 is a Reactome release 97 curated reaction event. The source record connects AA, O2 to HXA3/B3 in cytosol.", "equation": "AA + O2 → HXA3/B3", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2162031", "reactome_id": "R-ALL-2162031", "label": "HXA3/B3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "hydroperoxy icosatetraenoate isomerase activity of ALOX12:Fe2+ [cytosol]", "activity_id": "2161887", "ec_numbers": [ "5.4.4.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:11115402", "title": "Evidence for the presence of phospholipid hydroperoxide glutathione peroxidase in human platelets: implications for its involvement in the regulatory network of the 12-lipoxygenase pathway of arachidonic acid metabolism", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11115402/" }, { "id": "PMID:15123652", "title": "The rat leukocyte-type 12-lipoxygenase exhibits an intrinsic hepoxilin A3 synthase activity", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15123652/" } ], "x": 590, "y": 1730.6491228070174, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161795", "label": "Arachidonate is hydroxylated to 16-HETE by CYP(1)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:49973" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161795", "summary": "Arachidonate is hydroxylated to 16-HETE by CYP(1) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to NADP+, H2O, 16-HETE in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:49973.", "equation": "AA + O2 + NADPH + H+ → NADP+ + H2O + 16-HETE", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142667", "reactome_id": "R-ALL-2142667", "label": "16-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132019", "formula": "C20H31O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP(1) [endoplasmic reticulum membrane]", "activity_id": "2161998", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" } ], "x": 868.6666666666667, "y": 1646.157894736842, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161814", "label": "Arachidonate is hydroxylated to 19-HETE by CYP(2)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:39760" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161814", "summary": "Arachidonate is hydroxylated to 19-HETE by CYP(2) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to 19-HETE, NADP+, H2O in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:39760.", "equation": "AA + O2 + NADPH + H+ → 19-HETE + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142836", "reactome_id": "R-ALL-2142836", "label": "19-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76627", "formula": "C20H31O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP(2) [endoplasmic reticulum membrane]", "activity_id": "2162026", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9435160", "title": "Cytochromes P450 with bisallylic hydroxylation activity on arachidonic and linoleic acids studied with human recombinant enzymes and with human and rat liver microsomes", "journal": "J Pharmacol Exp Ther", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9435160/" }, { "id": "PMID:15611369", "title": "Functional variant of CYP4A11 20-hydroxyeicosatetraenoic acid synthase is associated with essential hypertension", "journal": "Circulation", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15611369/" }, { "id": "PMID:14660610", "title": "CYP2U1, a novel human thymus- and brain-specific cytochrome P450, catalyzes omega- and (omega-1)-hydroxylation of fatty acids", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14660610/" }, { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" } ], "x": 311.33333333333337, "y": 1649.1754385964912, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161844", "label": "15k-LXA4 is reduced to dhk-LXA4 by PTGR1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:41593" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161844", "summary": "15k-LXA4 is reduced to dhk-LXA4 by PTGR1 is a Reactome release 97 curated reaction event. The source record connects 15k-LXA4, H+, NADH to dhk-LXA4, NAD+ in cytosol and cross-references RHEA:41593.", "equation": "15k-LXA4 + H+ + NADH → dhk-LXA4 + NAD+", "inputs": [ { "node_id": "R-ALL-2142761", "reactome_id": "R-ALL-2142761", "label": "15k-LXA4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78311", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142690", "reactome_id": "R-ALL-2142690", "label": "dhk-LXA4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78325", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "13-lipoxin reductase activity of PTGR1 [cytosol]", "activity_id": "2161822", "ec_numbers": null, "accessions": [ "Q14914" ] } ], "citations": [ { "id": "PMID:8576264", "title": "cDNA cloning, expression, and mutagenesis study of leukotriene B4 12-hydroxydehydrogenase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8576264/" }, { "id": "PMID:10837478", "title": "Oxidoreductases in lipoxin A4 metabolic inactivation: a novel role for 15-oxoprostaglandin 13-reductase/leukotriene B4 12-hydroxydehydrogenase in inflammation", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10837478/" } ], "x": 5948.666666666667, "y": 2016.6309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161868", "label": "EXD4 is converted to EXE4 by DPEP", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161868", "summary": "EXD4 is converted to EXE4 by DPEP is a Reactome release 97 curated reaction event. The source record connects EXD4, H2O to EXE4, Gly in cytosol.", "equation": "EXD4 + H2O → EXE4 + Gly", "inputs": [ { "node_id": "R-ALL-2142758", "reactome_id": "R-ALL-2142758", "label": "EXD4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747583", "formula": "C25H39N2O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142730", "reactome_id": "R-ALL-2142730", "label": "EXE4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747194", "formula": "C23H36NO5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "eoxin E4 synthase activity of DPEP [cytosol]", "activity_id": "2161981", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18184802", "title": "Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and mast cells", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18184802/" }, { "id": "PMID:18647347", "title": "Hodgkin Reed-Sternberg cells express 15-lipoxygenase-1 and are putative producers of eoxins in vivo: novel insight into the inflammatory features of classical Hodgkin lymphoma", "journal": "FEBS J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18647347/" } ], "x": 868.6666666666667, "y": 1712.5438596491229, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161890", "label": "Arachidonate is epoxidated to 5,6-EET by CYP(4)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:49937" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161890", "summary": "Arachidonate is epoxidated to 5,6-EET by CYP(4) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to 5,6-EET, NADP+, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:49937.", "equation": "AA + O2 + NADPH + H+ → 5,6-EET + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142739", "reactome_id": "R-ALL-2142739", "label": "5,6-EET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:131992", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP(4) [endoplasmic reticulum membrane]", "activity_id": "2161927", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" }, { "id": "PMID:8631948", "title": "Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631948/" } ], "x": 590, "y": 1649.1754385964912, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161899", "label": "Arachidonate is epoxidated to 8,9-EET by CYP(5)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:64985" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161899", "summary": "Arachidonate is epoxidated to 8,9-EET by CYP(5) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to NADP+, H2O, 8,9-EET in endoplasmic reticulum membrane · cytosol and cross-references RHEA:64985.", "equation": "AA + O2 + NADPH + H+ → NADP+ + H2O + 8,9-EET", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142662", "reactome_id": "R-ALL-2142662", "label": "8,9-EET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84025", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP(5) [endoplasmic reticulum membrane]", "activity_id": "2161882", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" }, { "id": "PMID:9866708", "title": "Analysis of cytochrome P450 metabolites of arachidonic and linoleic acids by liquid chromatography-mass spectrometry with ion trap MS", "journal": "Anal Biochem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9866708/" }, { "id": "PMID:7625847", "title": "Arachidonic acid metabolism by human cytochrome P450s 2C8, 2C9, 2E1, and 1A2: regioselective oxygenation and evidence for a role for CYP2C enzymes in arachidonic acid epoxygenation in human liver microsomes", "journal": "Arch Biochem Biophys", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7625847/" }, { "id": "PMID:8631948", "title": "Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631948/" } ], "x": 868.6666666666667, "y": 1649.1754385964912, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161907", "label": "ALOX5 oxidises 15R-HETE to 15epi-LXA4/B4", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · nuclear envelope", "compartment_ids": [ "GO:0005829", "GO:0005635" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161907", "summary": "ALOX5 oxidises 15R-HETE to 15epi-LXA4/B4 is a Reactome release 97 curated reaction event. The source record connects 15R-HETE, O2 to 15epi-LXA4/B4 in cytosol · nuclear envelope.", "equation": "15R-HETE + O2 → 15epi-LXA4/B4", "inputs": [ { "node_id": "R-ALL-2142738", "reactome_id": "R-ALL-2142738", "label": "15R-HETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78837", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2161609", "reactome_id": "R-ALL-2161609", "label": "15epi-LXA4/B4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen of ALOX5:ALOX5AP:LTC4S [nuclear envelope]", "activity_id": "2161852", "ec_numbers": [ "1.13.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:7568157", "title": "Aspirin triggers previously undescribed bioactive eicosanoids by human endothelial cell-leukocyte interactions", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7568157/" } ], "x": 5670, "y": 2266.7023809523807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161917", "label": "ALOX5 oxidises 15S-HpETE to LXA4/B4", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161917", "summary": "ALOX5 oxidises 15S-HpETE to LXA4/B4 is a Reactome release 97 curated reaction event. The source record connects O2, 15S-HpETE to LXA4/B4 in cytosol.", "equation": "O2 + 2 15S-HpETE → 2 LXA4/B4", "inputs": [ { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2142718", "reactome_id": "R-ALL-2142718", "label": "15S-HpETE", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57446", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2161856", "reactome_id": "R-ALL-2161856", "label": "LXA4/B4", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen of ALOX5:ALOX5AP:LTC4S [nuclear envelope]", "activity_id": "2161852", "ec_numbers": [ "1.13.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:6089195", "title": "Lipoxins: novel series of biologically active compounds formed from arachidonic acid in human leukocytes", "journal": "Proc Natl Acad Sci U S A", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6089195/" }, { "id": "PMID:6422933", "title": "Trihydroxytetraenes: a novel series of compounds formed from arachidonic acid in human leukocytes", "journal": "Biochem Biophys Res Commun", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6422933/" }, { "id": "PMID:3579953", "title": "Lipoxin synthesis by arachidonate 5-lipoxygenase purified from porcine leukocytes", "journal": "Biochem Biophys Res Commun", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3579953/" }, { "id": "PMID:3770188", "title": "Evidence for a 5(6)-epoxytetraene intermediate in the biosynthesis of lipoxins in human leukocytes. Conversion into lipoxin A by cytosolic epoxide hydrolase", "journal": "FEBS Lett", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3770188/" } ], "x": 5948.666666666667, "y": 2266.7023809523807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161940", "label": "Arachidonate is hydroxylated to 20-HETE by CYP(3)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:39756" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161940", "summary": "Arachidonate is hydroxylated to 20-HETE by CYP(3) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to 20-HETE, NADP+, H2O in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:39756.", "equation": "AA + O2 + NADPH + H+ → 20-HETE + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142780", "reactome_id": "R-ALL-2142780", "label": "20-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76624", "formula": "C20H31O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP(3) [endoplasmic reticulum membrane]", "activity_id": "2161836", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15611369", "title": "Functional variant of CYP4A11 20-hydroxyeicosatetraenoic acid synthase is associated with essential hypertension", "journal": "Circulation", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15611369/" }, { "id": "PMID:9618440", "title": "Metabolism of arachidonic acid to 20-hydroxy-5,8,11, 14-eicosatetraenoic acid by P450 enzymes in human liver: involvement of CYP4F2 and CYP4A11", "journal": "J Pharmacol Exp Ther", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9618440/" }, { "id": "PMID:12432928", "title": "Prostaglandin and leukotriene omega-hydroxylases", "journal": "Prostaglandins Other Lipid Mediat", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12432928/" }, { "id": "PMID:14660610", "title": "CYP2U1, a novel human thymus- and brain-specific cytochrome P450, catalyzes omega- and (omega-1)-hydroxylation of fatty acids", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14660610/" }, { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" } ], "x": 311.33333333333337, "y": 1652.1929824561403, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161945", "label": "EXC4 is converted to EXD4 by GGT", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161945", "summary": "EXC4 is converted to EXD4 by GGT is a Reactome release 97 curated reaction event. The source record connects EXC4 to EXD4, L-Glu in cytosol.", "equation": "EXC4 → EXD4 + L-Glu", "inputs": [ { "node_id": "R-ALL-2142726", "reactome_id": "R-ALL-2142726", "label": "EXC4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63984", "formula": "C30H47N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142758", "reactome_id": "R-ALL-2142758", "label": "EXD4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747583", "formula": "C25H39N2O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "eoxin D4 synthase activity of GGT [cytosol]", "activity_id": "2161784", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18184802", "title": "Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and mast cells", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18184802/" }, { "id": "PMID:18647347", "title": "Hodgkin Reed-Sternberg cells express 15-lipoxygenase-1 and are putative producers of eoxins in vivo: novel insight into the inflammatory features of classical Hodgkin lymphoma", "journal": "FEBS J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18647347/" } ], "x": 590, "y": 1721.5964912280701, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161946", "label": "5S-HpETE is reduced to 5S-HETE by GPX1/2/4", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:48621" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161946", "summary": "5S-HpETE is reduced to 5S-HETE by GPX1/2/4 is a Reactome release 97 curated reaction event. The source record connects 5S-HpETE, GSH to 5S-HETE, GSSG, H2O in cytosol and cross-references RHEA:48621.", "equation": "5S-HpETE + 2 GSH → 5S-HETE + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-266010", "reactome_id": "R-ALL-266010", "label": "5S-HpETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57450", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142733", "reactome_id": "R-ALL-2142733", "label": "5S-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90632", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glutathione peroxidase activity of GPX1/2/4 [cytosol]", "activity_id": "2161908", "ec_numbers": [ "1.11.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:6816802", "title": "Role of glutathione peroxidase and hexose monophosphate shunt in the platelet lipoxygenase pathway", "journal": "J Biol Chem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6816802/" }, { "id": "PMID:10549853", "title": "Cellular glutathione peroxidase as a predominant scavenger of hydroperoxyeicosatetraenoic acids in rabbit alveolar macrophages", "journal": "Biol Pharm Bull", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10549853/" }, { "id": "PMID:11115402", "title": "Evidence for the presence of phospholipid hydroperoxide glutathione peroxidase in human platelets: implications for its involvement in the regulatory network of the 12-lipoxygenase pathway of arachidonic acid metabolism", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11115402/" }, { "id": "PMID:8428933", "title": "Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSHPx-GI", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8428933/" } ], "x": 311.33333333333337, "y": 1552.6140350877192, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161948", "label": "Arachidonate is converted to 12-oxoETE by ALOX12", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161948", "summary": "Arachidonate is converted to 12-oxoETE by ALOX12 is a Reactome release 97 curated reaction event. The source record connects AA to 12-oxoETE in cytosol.", "equation": "AA → 12-oxoETE", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142745", "reactome_id": "R-ALL-2142745", "label": "12-oxoETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:75231", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 12(S)-lipoxygenase activity of ALOX12:Fe2+ [cytosol]", "activity_id": "2161926", "ec_numbers": [ "1.13.11.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:10692117", "title": "Stereoselective biosynthesis of hepoxilin B3 in human epidermis", "journal": "J Invest Dermatol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10692117/" } ], "x": 868.6666666666667, "y": 1730.6491228070174, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161949", "label": "HXA3/B3 is hydrolysed to TrXA3/B3 by HXEH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50901" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161949", "summary": "HXA3/B3 is hydrolysed to TrXA3/B3 by HXEH is a Reactome release 97 curated reaction event. The source record connects HXA3/B3, H2O to TrXA3/B3 in cytosol and cross-references RHEA:50901.", "equation": "HXA3/B3 + H2O → TrXA3/B3", "inputs": [ { "node_id": "R-ALL-2162031", "reactome_id": "R-ALL-2162031", "label": "HXA3/B3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2161985", "reactome_id": "R-ALL-2161985", "label": "TrXA3/B3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "hepoxilin-epoxide hydrolase activity of HXEH [cytosol]", "activity_id": "2161802", "ec_numbers": [ "3.3.2.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:6406490", "title": "Arachidonic acid epoxides. Isolation and structure of two hydroxy epoxide intermediates in the formation of 8,11,12- and 10,11,12-trihydroxyeicosatrienoic acids", "journal": "J Biol Chem", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6406490/" }, { "id": "PMID:2722835", "title": "Purification of hepoxilin epoxide hydrolase from rat liver", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2722835/" }, { "id": "PMID:8829479", "title": "Characterization of arachidonic acid metabolites through the 12-lipoxygenase pathway in human epidermis by high-performance liquid chromatography and gas chromatography/mass spectrometry", "journal": "Rapid Commun Mass Spectrom", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8829479/" }, { "id": "PMID:9540966", "title": "Occurrence of hepoxilins and trioxilins in psoriatic lesions", "journal": "J Invest Dermatol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9540966/" } ], "x": 311.33333333333337, "y": 1694.438596491228, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161950", "label": "Arachidonate is oxidised to 12R-HpETE by ALOX12B", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:41337" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161950", "summary": "Arachidonate is oxidised to 12R-HpETE by ALOX12B is a Reactome release 97 curated reaction event. The source record connects AA, O2 to 12R-HpETE in cytosol and cross-references RHEA:41337.", "equation": "AA + O2 → 12R-HpETE", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142790", "reactome_id": "R-ALL-2142790", "label": "12R-HpETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:75230", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 12(S)-lipoxygenase activity of ALOX12B:Fe2+ [cytosol]", "activity_id": "2161752", "ec_numbers": [ "1.13.11.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:9618483", "title": "A 12R-lipoxygenase in human skin: mechanistic evidence, molecular cloning, and expression", "journal": "Proc Natl Acad Sci U S A", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9618483/" } ], "x": 590, "y": 1600.8947368421052, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161951", "label": "Arachidonate is oxidised to 15R-HETE by Acetyl-PTGS2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:52497" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161951", "summary": "Arachidonate is oxidised to 15R-HETE by Acetyl-PTGS2 is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to 15R-HETE, NADP+, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:52497.", "equation": "AA + O2 + NADPH + H+ → 15R-HETE + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142738", "reactome_id": "R-ALL-2142738", "label": "15R-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78837", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of Ac-PTGS2 dimer [endoplasmic reticulum membrane]", "activity_id": "2161919", "ec_numbers": [ "1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:7568157", "title": "Aspirin triggers previously undescribed bioactive eicosanoids by human endothelial cell-leukocyte interactions", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7568157/" } ], "x": 590, "y": 1652.1929824561403, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161959", "label": "12R-HpETE is reduced to 12R-HETE by GPX1/2/4", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:76692" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161959", "summary": "12R-HpETE is reduced to 12R-HETE by GPX1/2/4 is a Reactome release 97 curated reaction event. The source record connects 12R-HpETE, GSH to 12R-HETE, GSSG, H2O in cytosol and cross-references RHEA:76692.", "equation": "12R-HpETE + 2 GSH → 12R-HETE + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-2142790", "reactome_id": "R-ALL-2142790", "label": "12R-HpETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:75230", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142716", "reactome_id": "R-ALL-2142716", "label": "12R-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83343", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glutathione peroxidase activity of GPX1/2/4 [cytosol]", "activity_id": "2161908", "ec_numbers": [ "1.11.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:6816802", "title": "Role of glutathione peroxidase and hexose monophosphate shunt in the platelet lipoxygenase pathway", "journal": "J Biol Chem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6816802/" }, { "id": "PMID:11115402", "title": "Evidence for the presence of phospholipid hydroperoxide glutathione peroxidase in human platelets: implications for its involvement in the regulatory network of the 12-lipoxygenase pathway of arachidonic acid metabolism", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11115402/" }, { "id": "PMID:8428933", "title": "Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSHPx-GI", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8428933/" } ], "x": 590, "y": 1552.6140350877192, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161961", "label": "5,6-EET is hydrolysed to 5,6-DHET by EPHX2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161961", "summary": "5,6-EET is hydrolysed to 5,6-DHET by EPHX2 is a Reactome release 97 curated reaction event. The source record connects H2O, 5,6-EET to 5,6-DHET in cytosol.", "equation": "H2O + 5,6-EET → 5,6-DHET", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142739", "reactome_id": "R-ALL-2142739", "label": "5,6-EET", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:131992", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142682", "reactome_id": "R-ALL-2142682", "label": "5,6-DHET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63974", "formula": "C20H34O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of EPHX2 dimer [cytosol]", "activity_id": "2161846", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:12468260", "title": "Characterization and identification of cytochrome P450 metabolites of arachidonic acid released by human peritoneal macrophages obtained from the pouch of Douglas", "journal": "Prostaglandins Leukot Essent Fatty Acids", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12468260/" }, { "id": "PMID:15096040", "title": "Structure of human epoxide hydrolase reveals mechanistic inferences on bifunctional catalysis in epoxide and phosphate ester hydrolysis", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15096040/" } ], "x": 868.6666666666667, "y": 1721.5964912280701, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161962", "label": "LTA4 is hydrolysed to 6t-/6t,12epi-LTB4", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2161962", "summary": "LTA4 is hydrolysed to 6t-/6t,12epi-LTB4 is a Reactome release 97 curated reaction event. The source record connects LTA4, H2O to 6t/6t,12epi-LTB4 in cytosol.", "equation": "LTA4 + H2O → 6t/6t,12epi-LTB4", "inputs": [ { "node_id": "R-ALL-265281", "reactome_id": "R-ALL-265281", "label": "LTA4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2161801", "reactome_id": "R-ALL-2161801", "label": "6t/6t,12epi-LTB4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10741385", "title": "Inhibition of calcium ionophore-stimulated leukotriene generation from intact human neutrophils by captopril", "journal": "Res Commun Mol Pathol Pharmacol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10741385/" }, { "id": "PMID:2998743", "title": "Metabolism of leukotrienes by adult and fetal human lungs", "journal": "Exp Lung Res", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2998743/" } ], "x": 868.6666666666667, "y": 1742.719298245614, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161964", "label": "Arachidonate is oxidised to 12S-HpETE by ALOX12/15", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10429" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161964", "summary": "Arachidonate is oxidised to 12S-HpETE by ALOX12/15 is a Reactome release 97 curated reaction event. The source record connects AA, O2 to 12S-HpETE in cytosol and cross-references RHEA:10429.", "equation": "AA + O2 → 12S-HpETE", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142811", "reactome_id": "R-ALL-2142811", "label": "12S-HpETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57444", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 12(S)-lipoxygenase activity of ALOX12/15 [cytosol]", "activity_id": "2162014", "ec_numbers": [ "1.13.11.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:2377602", "title": "Molecular cloning, primary structure, and expression of the human platelet/erythroleukemia cell 12-lipoxygenase", "journal": "Proc Natl Acad Sci U S A", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2377602/" }, { "id": "PMID:2217179", "title": "Cloning of the cDNA for human 12-lipoxygenase", "journal": "Proc Natl Acad Sci U S A", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2217179/" }, { "id": "PMID:8334154", "title": "Overexpression, purification and characterization of human recombinant 15-lipoxygenase", "journal": "Biochim Biophys Acta", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8334154/" }, { "id": "PMID:2318885", "title": "Expression of cloned human reticulocyte 15-lipoxygenase and immunological evidence that 15-lipoxygenases of different cell types are related", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2318885/" } ], "x": 868.6666666666667, "y": 1600.8947368421052, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161979", "label": "20cho-LTB4 is oxidised to 20cooh-LTB4 by ALDH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:48673" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161979", "summary": "20cho-LTB4 is oxidised to 20cooh-LTB4 by ALDH is a Reactome release 97 curated reaction event. The source record connects 20cho-LTB4, NADPH, H+ to 20cooh-LTB4, NADP+, H2O in cytosol and cross-references RHEA:48673.", "equation": "20cho-LTB4 + NADPH + H+ + O2 → 20cooh-LTB4 + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-2142740", "reactome_id": "R-ALL-2142740", "label": "20cho-LTB4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90720", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2161582", "reactome_id": "R-ALL-2161582", "label": "20cooh-LTB4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90722", "formula": "C20H28O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "20-aldehyde-leukotriene B4 20-monooxygenase activity of ALDH [cytosol]", "activity_id": "2161683", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:2549038", "title": "Identification of an aldehyde dehydrogenase in the microsomes of human polymorphonuclear leukocytes that metabolizes 20-aldehyde leukotriene B4", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2549038/" } ], "x": 868.6666666666667, "y": 1652.1929824561403, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2161999", "label": "12S-HpETE is reduced to 12S-HETE by GPX1/2/4", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50709" ], "source_url": "https://reactome.org/content/detail/R-HSA-2161999", "summary": "12S-HpETE is reduced to 12S-HETE by GPX1/2/4 is a Reactome release 97 curated reaction event. The source record connects 12S-HpETE, GSH to 12S-HETE, GSSG, H2O in cytosol and cross-references RHEA:50709.", "equation": "12S-HpETE + 2 GSH → 12S-HETE + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-2142811", "reactome_id": "R-ALL-2142811", "label": "12S-HpETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57444", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142678", "reactome_id": "R-ALL-2142678", "label": "12S-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90680", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glutathione peroxidase activity of GPX1/2/4 [cytosol]", "activity_id": "2161908", "ec_numbers": [ "1.11.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:6816802", "title": "Role of glutathione peroxidase and hexose monophosphate shunt in the platelet lipoxygenase pathway", "journal": "J Biol Chem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6816802/" }, { "id": "PMID:11115402", "title": "Evidence for the presence of phospholipid hydroperoxide glutathione peroxidase in human platelets: implications for its involvement in the regulatory network of the 12-lipoxygenase pathway of arachidonic acid metabolism", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11115402/" }, { "id": "PMID:8428933", "title": "Expression, characterization, and tissue distribution of a new cellular selenium-dependent glutathione peroxidase, GSHPx-GI", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8428933/" } ], "x": 868.6666666666667, "y": 1552.6140350877192, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162002", "label": "Arachidonate is oxidised to 15S-HpETE by ALOX15/15B", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16870" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162002", "summary": "Arachidonate is oxidised to 15S-HpETE by ALOX15/15B is a Reactome release 97 curated reaction event. The source record connects AA, O2 to 15S-HpETE in cytosol and cross-references RHEA:16870.", "equation": "AA + O2 → 15S-HpETE", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142718", "reactome_id": "R-ALL-2142718", "label": "15S-HpETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57446", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15/15B [cytosol]", "activity_id": "2161832", "ec_numbers": [ "1.13.11.33" ], "accessions": [] } ], "citations": [ { "id": "PMID:17662651", "title": "Expression of 15-lipoxygenase type-1 in human mast cells", "journal": "Biochim Biophys Acta", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17662651/" }, { "id": "PMID:8334154", "title": "Overexpression, purification and characterization of human recombinant 15-lipoxygenase", "journal": "Biochim Biophys Acta", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8334154/" }, { "id": "PMID:1662607", "title": "Purification of two forms of arachidonate 15-lipoxygenase from human leukocytes", "journal": "Eur J Biochem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1662607/" }, { "id": "PMID:11839751", "title": "Evidence that arachidonate 15-lipoxygenase 2 is a negative cell cycle regulator in normal prostate epithelial cells", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11839751/" }, { "id": "PMID:18570379", "title": "Substrate specificity changes for human reticulocyte and epithelial 15-lipoxygenases reveal allosteric product regulation", "journal": "Biochemistry", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18570379/" } ], "x": 311.33333333333337, "y": 1603.9122807017543, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162019", "label": "LTA4 is converted to EXA4 by ALOX15", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2162019", "summary": "LTA4 is converted to EXA4 by ALOX15 is a Reactome release 97 curated reaction event. The source record connects LTA4 to EXA4 in cytosol.", "equation": "LTA4 → EXA4", "inputs": [ { "node_id": "R-ALL-265281", "reactome_id": "R-ALL-265281", "label": "LTA4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142772", "reactome_id": "R-ALL-2142772", "label": "EXA4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132070", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "leukotriene-C4 synthase activity of ALOX15:Fe2+ [cytosol]", "activity_id": "2161993", "ec_numbers": [ "4.4.1.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:18184802", "title": "Eoxins are proinflammatory arachidonic acid metabolites produced via the 15-lipoxygenase-1 pathway in human eosinophils and mast cells", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18184802/" }, { "id": "PMID:18647347", "title": "Hodgkin Reed-Sternberg cells express 15-lipoxygenase-1 and are putative producers of eoxins in vivo: novel insight into the inflammatory features of classical Hodgkin lymphoma", "journal": "FEBS J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18647347/" } ], "x": 311.33333333333337, "y": 1733.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162186", "label": "COQ3 methylates DeMQ10H2", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:44412", "RHEA:44413" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162186", "summary": "COQ3 methylates DeMQ10H2 is a Reactome release 97 curated reaction event. The source record connects DeMQ10H2, AdoMet to CoQH2, AdoHcy, H+ in mitochondrial matrix and cross-references RHEA:44412, RHEA:44413.", "equation": "DeMQ10H2 + AdoMet → CoQH2 + AdoHcy + H+", "inputs": [ { "node_id": "R-ALL-2162212", "reactome_id": "R-ALL-2162212", "label": "DeMQ10H2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64182", "formula": "C58H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162265", "reactome_id": "R-ALL-2162265", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2162198", "reactome_id": "R-ALL-2162198", "label": "CoQH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162252", "reactome_id": "R-ALL-2162252", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-demethylubiquinol 3-O-methyltransferase activity of COQ3:COQ4:COQ5:COQ6:COQ7:COQ9 [mitochondrial inner membrane]", "activity_id": "2162293", "ec_numbers": [ "2.1.1.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:10777520", "title": "Isolation and functional expression of human COQ3, a gene encoding a methyltransferase required for ubiquinone biosynthesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777520/" }, { "id": "PMID:26251450", "title": "Structural and biochemical studies reveal UbiG/Coq3 as a class of novel membrane-binding proteins", "journal": "Biochem J", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26251450/" }, { "id": "PMID:38425362", "title": "In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis", "journal": "Nat Catal", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/38425362/" } ], "x": 4730, "y": 3298.2083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162187", "label": "COQ6 hydroxylates DHB", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2162187", "summary": "COQ6 hydroxylates DHB is a Reactome release 97 curated reaction event. The source record connects DHB, O2, H+ to DHDB, H2O, (2Fe-2S)2+ in mitochondrial inner membrane · mitochondrial matrix.", "equation": "DHB + O2 + H+ + (2Fe-2S)(1+) → DHDB + H2O + (2Fe-2S)2+", "inputs": [ { "node_id": "R-ALL-2162207", "reactome_id": "R-ALL-2162207", "label": "DHB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:84503", "formula": "C57H85O3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2395504", "reactome_id": "R-ALL-2395504", "label": "(2Fe-2S)(1+)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33738", "formula": "Fe2S2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2162209", "reactome_id": "R-ALL-2162209", "label": "DHDB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62793", "formula": "C57H85O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2395501", "reactome_id": "R-ALL-2395501", "label": "(2Fe-2S)2+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33737", "formula": "Fe2S2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen of COQ3:COQ4…", "activity_id": "2162332", "ec_numbers": [ "1.14.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:21540551", "title": "COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness", "journal": "J Clin Invest", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21540551/" }, { "id": "PMID:21944752", "title": "Coenzyme Q biosynthesis: Coq6 is required for the C5-hydroxylation reaction and substrate analogs rescue Coq6 deficiency", "journal": "Chem Biol", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21944752/" }, { "id": "PMID:12721307", "title": "The Saccharomyces cerevisiae COQ6 gene encodes a mitochondrial flavin-dependent monooxygenase required for coenzyme Q biosynthesis", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12721307/" }, { "id": "PMID:27499296", "title": "Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function", "journal": "Mol Cell", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27499296/" }, { "id": "PMID:24270420", "title": "ADCK4 mutations promote steroid-resistant nephrotic syndrome through CoQ10 biosynthesis disruption", "journal": "J Clin Invest", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24270420/" }, { "id": "PMID:37476682", "title": "Adolescence Onset Primary Coenzyme Q10 Deficiency With Rare CoQ8A Gene Mutation: A Case Report and Review of Literature", "journal": "Clin Med Insights Case Rep", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37476682/" } ], "x": 4730, "y": 3427.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162188", "label": "COQ5 methylates MDMQ10H2", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:44764", "RHEA:44765" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162188", "summary": "COQ5 methylates MDMQ10H2 is a Reactome release 97 curated reaction event. The source record connects MDMQ10H2, AdoMet to DMQ10H2, AdoHcy, H+ in mitochondrial matrix and cross-references RHEA:44764, RHEA:44765.", "equation": "MDMQ10H2 + AdoMet → DMQ10H2 + AdoHcy + H+", "inputs": [ { "node_id": "R-ALL-2162205", "reactome_id": "R-ALL-2162205", "label": "MDMQ10H2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64180", "formula": "C57H88O3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162265", "reactome_id": "R-ALL-2162265", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2162204", "reactome_id": "R-ALL-2162204", "label": "DMQ10H2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64181", "formula": "C58H90O3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162252", "reactome_id": "R-ALL-2162252", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-methoxy-6-polyprenyl-1,4-benzoquinol methyltransferase activity of COQ3:COQ4:COQ5:COQ6:COQ7:COQ9 [mitochondrial inner membrane]", "activity_id": "2167835", "ec_numbers": [ "2.1.1.201" ], "accessions": [] } ], "citations": [ { "id": "PMID:9083049", "title": "Characterization of the COQ5 gene from Saccharomyces cerevisiae. Evidence for a C-methyltransferase in ubiquinone biosynthesis", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9083049/" }, { "id": "PMID:25152161", "title": "Molecular characterization of the human COQ5 C-methyltransferase in coenzyme Q10 biosynthesis", "journal": "Biochim Biophys Acta", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25152161/" }, { "id": "PMID:27155576", "title": "Disruption of the human COQ5-containing protein complex is associated with diminished coenzyme Q10 levels under two different conditions of mitochondrial energy deficiency", "journal": "Biochim Biophys Acta", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27155576/" }, { "id": "PMID:23354120", "title": "Detection of suppressed maturation of the human COQ5 protein in the mitochondria following mitochondrial uncoupling by an antibody recognizing both precursor and mature forms of COQ5", "journal": "Mitochondrion", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23354120/" }, { "id": "PMID:38425362", "title": "In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis", "journal": "Nat Catal", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/38425362/" } ], "x": 5008.666666666667, "y": 3298.2083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162191", "label": "Unknown enzyme hydroxylates DMPhOH", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2162191", "summary": "Unknown enzyme hydroxylates DMPhOH is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DMPhOH, O2, NADPH to MDMQ10H2, NADP+, H2O in mitochondrial matrix.", "equation": "DMPhOH + O2 + NADPH + H+ → MDMQ10H2 + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-2162208", "reactome_id": "R-ALL-2162208", "label": "DMPhOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:50774", "formula": "C57H88O2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2162205", "reactome_id": "R-ALL-2162205", "label": "MDMQ10H2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64180", "formula": "C57H88O3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of DMPhOH monooxygenase [mitochondrial matrix]", "activity_id": "2167847", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:21944752", "title": "Coenzyme Q biosynthesis: Coq6 is required for the C5-hydroxylation reaction and substrate analogs rescue Coq6 deficiency", "journal": "Chem Biol", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21944752/" }, { "id": "PMID:12721307", "title": "The Saccharomyces cerevisiae COQ6 gene encodes a mitochondrial flavin-dependent monooxygenase required for coenzyme Q biosynthesis", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12721307/" }, { "id": "PMID:38014142", "title": "COQ4 is required for the oxidative decarboxylation of the C1 carbon of Coenzyme Q in eukaryotic cells", "journal": "bioRxiv", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/38014142/" }, { "id": "PMID:38425362", "title": "In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis", "journal": "Nat Catal", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/38425362/" } ], "x": 4730, "y": 3525.125, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-2162192", "label": "COQ2 ligates all-E-10PrP2 to PHB", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:44564", "RHEA:44565" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162192", "summary": "COQ2 ligates all-E-10PrP2 to PHB is a Reactome release 97 curated reaction event. The source record connects PHB, all-E-10PrP2 to DHB, PPi in mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:44564, RHEA:44565.", "equation": "PHB + all-E-10PrP2 → DHB + PPi", "inputs": [ { "node_id": "R-ALL-2162213", "reactome_id": "R-ALL-2162213", "label": "PHB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17879", "formula": "C7H5O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162214", "reactome_id": "R-ALL-2162214", "label": "all-E-10PrP2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60721", "formula": "C50H81O7P2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2162207", "reactome_id": "R-ALL-2162207", "label": "DHB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84503", "formula": "C57H85O3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "4-hydroxybenzoate polyprenyltransferase activity of COQ2 [mitochondrial inner membrane]", "activity_id": "2162263", "ec_numbers": [ "2.5.1.39" ], "accessions": [ "Q96H96" ] } ], "citations": [ { "id": "PMID:15153069", "title": "Isolation and functional expression of human COQ2, a gene encoding a polyprenyl transferase involved in the synthesis of CoQ", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15153069/" }, { "id": "PMID:17916383", "title": "Investigation of coenzyme Q biosynthesis in human fibroblast and HepG2 cells", "journal": "J Biochem Biophys Methods", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17916383/" }, { "id": "PMID:17374725", "title": "Missense mutation of the COQ2 gene causes defects of bioenergetics and de novo pyrimidine synthesis", "journal": "Hum Mol Genet", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17374725/" } ], "x": 4730, "y": 3330.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162193", "label": "COQ3 methylates DHDB", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:44492", "RHEA:44493" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162193", "summary": "COQ3 methylates DHDB is a Reactome release 97 curated reaction event. The source record connects DHDB, AdoMet to MHDB, AdoHcy, H+ in mitochondrial matrix and cross-references RHEA:44492, RHEA:44493.", "equation": "DHDB + AdoMet → MHDB + AdoHcy + H+", "inputs": [ { "node_id": "R-ALL-2162209", "reactome_id": "R-ALL-2162209", "label": "DHDB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62793", "formula": "C57H85O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162265", "reactome_id": "R-ALL-2162265", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2162215", "reactome_id": "R-ALL-2162215", "label": "MHDB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62796", "formula": "C58H87O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162252", "reactome_id": "R-ALL-2162252", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "polyprenyldihydroxybenzoate methyltransferase activity of COQ3:COQ4:COQ5:COQ6:COQ7:COQ9 [mitochondrial inner membrane]", "activity_id": "2162287", "ec_numbers": [ "2.1.1.114" ], "accessions": [] } ], "citations": [ { "id": "PMID:10777520", "title": "Isolation and functional expression of human COQ3, a gene encoding a methyltransferase required for ubiquinone biosynthesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777520/" }, { "id": "PMID:26251450", "title": "Structural and biochemical studies reveal UbiG/Coq3 as a class of novel membrane-binding proteins", "journal": "Biochem J", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26251450/" }, { "id": "PMID:38425362", "title": "In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis", "journal": "Nat Catal", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/38425362/" } ], "x": 4451.333333333333, "y": 3330.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162194", "label": "COQ7:COQ9 octamer hydroxylates DMQ10H2", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2162194", "summary": "COQ7:COQ9 octamer hydroxylates DMQ10H2 is a Reactome release 97 curated reaction event. The source record connects DMQ10H2, O2, H+ to DeMQ10H2, H2O, NAD+ in mitochondrial inner membrane · mitochondrial matrix.", "equation": "DMQ10H2 + O2 + H+ + NADH → DeMQ10H2 + H2O + NAD+", "inputs": [ { "node_id": "R-ALL-2162204", "reactome_id": "R-ALL-2162204", "label": "DMQ10H2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64181", "formula": "C58H90O3", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2162212", "reactome_id": "R-ALL-2162212", "label": "DeMQ10H2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64182", "formula": "C58H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen of COQ3:COQ4…", "activity_id": "2167828", "ec_numbers": [ "1.14.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:10501970", "title": "Conservation of the Caenorhabditis elegans timing gene clk-1 from yeast to human: a gene required for ubiquinone biosynthesis with potential implications for aging", "journal": "Mamm Genome", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10501970/" }, { "id": "PMID:8621692", "title": "The COQ7 gene encodes a protein in Saccharomyces cerevisiae necessary for ubiquinone biosynthesis", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8621692/" }, { "id": "PMID:16624818", "title": "Complementation of Saccharomyces cerevisiae coq7 mutants by mitochondrial targeting of the Escherichia coli UbiF polypeptide: two functions of yeast Coq7 polypeptide in coenzyme Q biosynthesis", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16624818/" }, { "id": "PMID:25339443", "title": "Mitochondrial COQ9 is a lipid-binding protein that associates with COQ7 to enable coenzyme Q biosynthesis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25339443/" }, { "id": "PMID:36306796", "title": "Structure and functionality of a multimeric human COQ7:COQ9 complex", "journal": "Mol Cell", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36306796/" }, { "id": "PMID:27499296", "title": "Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function", "journal": "Mol Cell", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27499296/" } ], "x": 5008.666666666667, "y": 3427.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162195", "label": "COQ4 decarboxylates MHDB", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:81275" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162195", "summary": "COQ4 decarboxylates MHDB is a Reactome release 97 curated reaction event. The source record connects MHDB, H+ to DMPhOH, CO2 in mitochondrial matrix and cross-references RHEA:81275.", "equation": "MHDB + H+ → DMPhOH + CO2", "inputs": [ { "node_id": "R-ALL-2162215", "reactome_id": "R-ALL-2162215", "label": "MHDB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62796", "formula": "C58H87O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2162208", "reactome_id": "R-ALL-2162208", "label": "DMPhOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:50774", "formula": "C57H88O2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "4-hydroxy-3-methoxy-5-polyprenylbenzoate decarboxylase activity of COQ3:COQ4:COQ5:COQ6:COQ7:COQ9 [mitochondrial inner membrane]", "activity_id": "2167850", "ec_numbers": [ "4.1.1.130" ], "accessions": [] } ], "citations": [ { "id": "PMID:38014142", "title": "COQ4 is required for the oxidative decarboxylation of the C1 carbon of Coenzyme Q in eukaryotic cells", "journal": "bioRxiv", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/38014142/" }, { "id": "PMID:38425362", "title": "In vitro construction of the COQ metabolon unveils the molecular determinants of coenzyme Q biosynthesis", "journal": "Nat Catal", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/38425362/" } ], "x": 5008.666666666667, "y": 3395.4583333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162225", "label": "HEXA cleaves the terminal GlcNAc from small HA fragments", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2162225", "summary": "HEXA cleaves the terminal GlcNAc from small HA fragments is a Reactome release 97 curated reaction event. The source record connects H2O, GlcNAc-GlcA-GlcNAc to GlcNAc, GlcA-β1,3-GlcNAc in lysosomal lumen.", "equation": "H2O + GlcNAc-GlcA-GlcNAc → GlcNAc + GlcA-β1,3-GlcNAc", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2162223", "reactome_id": "R-ALL-2162223", "label": "GlcNAc-GlcA-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1678743", "reactome_id": "R-ALL-1678743", "label": "GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17411", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162224", "reactome_id": "R-ALL-2162224", "label": "GlcA-β1,3-GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64024", "formula": "C14H23NO12", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXA [lysosomal lumen]", "activity_id": "1605773", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:2971395", "title": "Oligosaccharide structure and amino acid sequence of the major glycopeptides of mature human beta-hexosaminidase", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2971395/" } ], "x": 2660, "y": 1007.2272727272727, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162226", "label": "GUSB tetramer hydrolyzes GlcA-β1,3-GlcNAc", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [ "RHEA:17634" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162226", "summary": "GUSB tetramer hydrolyzes GlcA-β1,3-GlcNAc is a Reactome release 97 curated reaction event. The source record connects H2O, GlcA-β1,3-GlcNAc to GlcA, GlcNAc in lysosomal lumen and cross-references RHEA:17634.", "equation": "H2O + GlcA-β1,3-GlcNAc → GlcA + GlcNAc", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2162224", "reactome_id": "R-ALL-2162224", "label": "GlcA-β1,3-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64024", "formula": "C14H23NO12", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1678868", "reactome_id": "R-ALL-1678868", "label": "GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4178", "formula": "C6H10O7", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1678743", "reactome_id": "R-ALL-1678743", "label": "GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17411", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-glucuronidase activity of GUSB tetramer [lysosomal lumen]", "activity_id": "1678848", "ec_numbers": [ "3.2.1.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:3468507", "title": "Cloning, sequencing, and expression of cDNA for human beta-glucuronidase", "journal": "Proc Natl Acad Sci U S A", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3468507/" }, { "id": "PMID:11568288", "title": "A novel inhibitor of beta-glucuronidase: L-aspartic acid", "journal": "Pediatr Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11568288/" } ], "x": 2938.666666666667, "y": 783.2575757575758, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162227", "label": "GUSB tetramer hydrolyses (HA)2", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [ "RHEA:17634" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162227", "summary": "GUSB tetramer hydrolyses (HA)2 is a Reactome release 97 curated reaction event. The source record connects H2O, (HA)2 to GlcA, GlcNAc-GlcA-GlcNAc in lysosomal lumen and cross-references RHEA:17634.", "equation": "H2O + (HA)2 → GlcA + GlcNAc-GlcA-GlcNAc", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2160866", "reactome_id": "R-ALL-2160866", "label": "(HA)2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Polymer", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1678868", "reactome_id": "R-ALL-1678868", "label": "GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4178", "formula": "C6H10O7", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162223", "reactome_id": "R-ALL-2162223", "label": "GlcNAc-GlcA-GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "beta-glucuronidase activity of GUSB tetramer [lysosomal lumen]", "activity_id": "1678848", "ec_numbers": [ "3.2.1.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:3468507", "title": "Cloning, sequencing, and expression of cDNA for human beta-glucuronidase", "journal": "Proc Natl Acad Sci U S A", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3468507/" }, { "id": "PMID:11568288", "title": "A novel inhibitor of beta-glucuronidase: L-aspartic acid", "journal": "Pediatr Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11568288/" } ], "x": 2381.3333333333335, "y": 901.1363636363636, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2162229", "label": "SLC17A5 exports GlcA from the lysosome to the cytosol", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal membrane", "compartment_ids": [ "GO:0005765" ], "rhea_ids": [ "RHEA:72591" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162229", "summary": "SLC17A5 exports GlcA from the lysosome to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects GlcA, H+ to GlcA, H+ in lysosomal membrane and cross-references RHEA:72591.", "equation": "GlcA + H+ → GlcA + H+", "inputs": [ { "node_id": "R-ALL-1678868", "reactome_id": "R-ALL-1678868", "label": "GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:4178", "formula": "C6H10O7", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428040", "reactome_id": "R-ALL-428040", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2162232", "reactome_id": "R-ALL-2162232", "label": "GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58720", "formula": "C6H9O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucuronate transmembrane transporter activity of SLC17A5 [lysosomal membrane]", "activity_id": "9941025", "ec_numbers": null, "accessions": [ "Q9NRA2" ] } ], "citations": [ { "id": "PMID:15503855", "title": "Hyaluronan catabolism: a new metabolic pathway", "journal": "Eur J Cell Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15503855/" }, { "id": "PMID:14514708", "title": "Devising a pathway for hyaluronan catabolism: are we there yet?", "journal": "Glycobiology", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14514708/" }, { "id": "PMID:15510212", "title": "Functional characterization of wild-type and mutant human sialin", "journal": "EMBO J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15510212/" } ], "x": 2381.3333333333335, "y": 795.0454545454545, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-2162253", "label": "PDSS1,2 ligates FPP to IPPP", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:27802", "RHEA:27803" ], "source_url": "https://reactome.org/content/detail/R-HSA-2162253", "summary": "PDSS1,2 ligates FPP to IPPP is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects FPP, IPPP to all-E-10PrP2, PPi in mitochondrial matrix and cross-references RHEA:27802, RHEA:27803.", "equation": "FPP + 7 IPPP → all-E-10PrP2 + 7 PPi", "inputs": [ { "node_id": "R-ALL-2162257", "reactome_id": "R-ALL-2162257", "label": "FPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:175763", "formula": "C15H25O7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162256", "reactome_id": "R-ALL-2162256", "label": "IPPP", "role": "input", "stoichiometry": 7, "chebi_id": "CHEBI:128769", "formula": "C5H9O7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2162214", "reactome_id": "R-ALL-2162214", "label": "all-E-10PrP2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60721", "formula": "C50H81O7P2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 7, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "all-trans-decaprenyl-diphosphate synthase activity of PDSS1/2 tetramer [mitochondrial matrix]", "activity_id": "2162261", "ec_numbers": [ "2.5.1.91" ], "accessions": [] } ], "citations": [ { "id": "PMID:16262699", "title": "Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans", "journal": "FEBS J", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16262699/" }, { "id": "PMID:17916383", "title": "Investigation of coenzyme Q biosynthesis in human fibroblast and HepG2 cells", "journal": "J Biochem Biophys Methods", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17916383/" } ], "x": 5008.666666666667, "y": 3330.625, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-217255", "label": "FMO1:FAD N-oxidises TAM", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-217255", "summary": "FMO1:FAD N-oxidises TAM is a Reactome release 97 curated reaction event. The source record connects O2, NADPH, H+ to TAMO, H2O, NADP+ in smooth endoplasmic reticulum.", "equation": "O2 + NADPH + H+ + TAM → TAMO + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9838650", "reactome_id": "R-ALL-9838650", "label": "TAM", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "ChemicalDrug", "currency": false } ], "outputs": [ { "node_id": "R-ALL-217269", "reactome_id": "R-ALL-217269", "label": "TAMO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63825", "formula": "C26H29NO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N,N-dimethylaniline monooxygenase activity of FMO1:FAD [endoplasmic reticulum membrane]", "activity_id": "217265", "ec_numbers": [ "1.14.13.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:15987777", "title": "Oxidation of tamoxifen by human flavin-containing monooxygenase (FMO) 1 and FMO3 to tamoxifen-N-oxide and its novel reduction back to tamoxifen by human cytochromes P450 and hemoglobin", "journal": "Drug Metab Dispos", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15987777/" } ], "x": 8018.666666666667, "y": 3050.892156862745, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-217258", "label": "FMO2:FAD:Mg2+ S-oxidises MTZ", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-217258", "summary": "FMO2:FAD:Mg2+ S-oxidises MTZ is a Reactome release 97 curated reaction event. The source record connects MTZ, NADPH, H+ to MTZ-SOX, NADP+, H2O in smooth endoplasmic reticulum.", "equation": "MTZ + NADPH + H+ + O2 → MTZ-SOX + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-217315", "reactome_id": "R-ALL-217315", "label": "MTZ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:50673", "formula": "C4H6N2S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-217320", "reactome_id": "R-ALL-217320", "label": "MTZ-SOX", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63828", "formula": "C4H6N2OS", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of FMO2:FAD:Mg2+ [endoplasmic reticulum membrane]", "activity_id": "217318", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11744609", "title": "Identification of active flavin-containing monooxygenase isoform 2 in human lung and characterization of expressed protein", "journal": "Drug Metab Dispos", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11744609/" } ], "x": 7461.333333333333, "y": 3012.7549019607845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2248891", "label": "M6PR transports activated ARSA to the lysosome", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "endoplasmic reticulum lumen · lysosomal lumen", "compartment_ids": [ "GO:0005788", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2248891", "summary": "M6PR transports activated ARSA to the lysosome is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects active ARSA:Ca2+ to active ARSA:Ca2+ in endoplasmic reticulum lumen · lysosomal lumen.", "equation": "active ARSA:Ca2+ → active ARSA:Ca2+", "inputs": [ { "node_id": "R-HSA-1614355", "reactome_id": "R-HSA-1614355", "label": "active ARSA:Ca2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1606814", "reactome_id": "R-HSA-1606814", "label": "active ARSA:Ca2+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of M6PR [transport vesicle]", "activity_id": "9841177", "ec_numbers": null, "accessions": [ "P20645" ] } ], "citations": [ { "id": "PMID:2960521", "title": "Mr 46,000 mannose 6-phosphate specific receptor: its role in targeting of lysosomal enzymes", "journal": "EMBO J", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2960521/" }, { "id": "PMID:1352293", "title": "In vitro mutagenesis of potential N-glycosylation sites of arylsulfatase A. Effects on glycosylation, phosphorylation, and intracellular sorting", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1352293/" } ], "x": 3321.3333333333335, "y": 1884.5961538461538, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-2299725", "label": "PGH2 diffuses from the endoplasmic reticulum lumen to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005788", "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2299725", "summary": "PGH2 diffuses from the endoplasmic reticulum lumen to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PGH2 to PGH2 in endoplasmic reticulum lumen · endoplasmic reticulum membrane · cytosol.", "equation": "PGH2 → PGH2", "inputs": [ { "node_id": "R-ALL-30138", "reactome_id": "R-ALL-30138", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-265283", "reactome_id": "R-ALL-265283", "label": "PGH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20346915", "title": "The prostaglandin transporter PGT transports PGH(2)", "journal": "Biochem. Biophys. Res. Commun.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20346915/" } ], "x": 868.6666666666667, "y": 1757.8070175438597, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-2309773", "label": "PGG2 is reduced to PGH2 by PTGS2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:42601", "RHEA:42600" ], "source_url": "https://reactome.org/content/detail/R-HSA-2309773", "summary": "PGG2 is reduced to PGH2 by PTGS2 is a Reactome release 97 curated reaction event. The source record connects PGG2, hydrogen donor to H2O, PGH2, hydrogen acceptor in endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:42601, RHEA:42600.", "equation": "PGG2 + hydrogen donor → H2O + PGH2 + hydrogen acceptor", "inputs": [ { "node_id": "R-ALL-140357", "reactome_id": "R-ALL-140357", "label": "PGG2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:82629", "formula": "C20H31O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9915557", "reactome_id": "R-ALL-9915557", "label": "hydrogen donor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17499", "formula": "H2A", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30138", "reactome_id": "R-ALL-30138", "label": "PGH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9915551", "reactome_id": "R-ALL-9915551", "label": "hydrogen acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13193", "formula": "A", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "peroxidase activity of PTGS2 dimer [endoplasmic reticulum membrane]", "activity_id": "2309777", "ec_numbers": [ "1.11.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:4514999", "title": "Detection and isolation of an endoperoxide intermediate in prostaglandin biosynthesis", "journal": "Proc Natl Acad Sci U S A", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4514999/" }, { "id": "PMID:1380156", "title": "Human cyclooxygenase-2 cDNA", "journal": "Proc Natl Acad Sci U S A", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1380156/" }, { "id": "PMID:9139685", "title": "Differential allosteric regulation of prostaglandin H synthase 1 and 2 by arachidonic acid", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9139685/" }, { "id": "PMID:7947975", "title": "Purification, characterization and selective inhibition of human prostaglandin G/H synthase 1 and 2 expressed in the baculovirus system", "journal": "Biochim Biophys Acta", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7947975/" } ], "x": 311.33333333333337, "y": 1555.6315789473683, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2309779", "label": "PTGS2 dimer binds PTGS2 inhibitors", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2309779", "summary": "PTGS2 dimer binds PTGS2 inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PTGS2 dimer, PTGS2 inhibitors to PTGS2 dimer:PTGS2 inhibitors in cytosol · endoplasmic reticulum membrane.", "equation": "PTGS2 dimer + PTGS2 inhibitors → PTGS2 dimer:PTGS2 inhibitors", "inputs": [ { "node_id": "R-HSA-140491", "reactome_id": "R-HSA-140491", "label": "PTGS2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9716714", "reactome_id": "R-ALL-9716714", "label": "PTGS2 inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2309778", "reactome_id": "R-HSA-2309778", "label": "PTGS2 dimer:PTGS2 inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10966456", "title": "Cyclooxygenases: structural, cellular, and molecular biology", "journal": "Annu Rev Biochem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10966456/" }, { "id": "PMID:8901870", "title": "Flexibility of the NSAID binding site in the structure of human cyclooxygenase-2", "journal": "Nat. Struct. Biol.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8901870/" }, { "id": "PMID:21467029", "title": "Human cyclooxygenase-2 is a sequence homodimer that functions as a conformational heterodimer", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21467029/" } ], "x": 311.33333333333337, "y": 1748.7543859649122, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-2309787", "label": "Arachidonate is oxidised to PGG2 by PTGS2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:42597" ], "source_url": "https://reactome.org/content/detail/R-HSA-2309787", "summary": "Arachidonate is oxidised to PGG2 by PTGS2 is a Reactome release 97 curated reaction event. The source record connects O2, AA to PGG2 in endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:42597.", "equation": "2 O2 + AA → PGG2", "inputs": [ { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-140357", "reactome_id": "R-ALL-140357", "label": "PGG2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:82629", "formula": "C20H31O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "prostaglandin-endoperoxide synthase activity of PTGS2 dimer [endoplasmic reticulum membrane]", "activity_id": "2309772", "ec_numbers": [ "1.14.99.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:4521806", "title": "Isolation and structure of two prostaglandin endoperoxides that cause platelet aggregation", "journal": "Proc Natl Acad Sci U S A", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4521806/" }, { "id": "PMID:4776443", "title": "Isolation and properties of intermediates in prostaglandin biosynthesis", "journal": "Biochim Biophys Acta", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4776443/" } ], "x": 590, "y": 1603.9122807017543, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2314678", "label": "ASA- acetylates PTGS1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2314678", "summary": "ASA- acetylates PTGS1 is a Reactome release 97 curated reaction event. The source record connects PTGS1 dimer, ASA- to Ac-PTGS1 dimer, ST in cytosol · endoplasmic reticulum membrane.", "equation": "PTGS1 dimer + ASA- → Ac-PTGS1 dimer + ST", "inputs": [ { "node_id": "R-HSA-428986", "reactome_id": "R-HSA-428986", "label": "PTGS1 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-2314693", "reactome_id": "R-ALL-2314693", "label": "ASA-", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2314695", "reactome_id": "R-HSA-2314695", "label": "Ac-PTGS1 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-2314690", "reactome_id": "R-ALL-2314690", "label": "ST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30762", "formula": "C7H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8175750", "title": "Acetylation of human prostaglandin endoperoxide synthase-2 (cyclooxygenase-2) by aspirin", "journal": "J. Biol. Chem.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8175750/" }, { "id": "PMID:7552725", "title": "The structural basis of aspirin activity inferred from the crystal structure of inactivated prostaglandin H2 synthase", "journal": "Nat. Struct. Biol.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7552725/" } ], "x": 311.33333333333337, "y": 1745.7368421052631, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2314686", "label": "ASA- acetylates PTGS2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2314686", "summary": "ASA- acetylates PTGS2 is a Reactome release 97 curated reaction event. The source record connects PTGS2 dimer, ASA- to Ac-PTGS2 dimer, ST in cytosol · endoplasmic reticulum membrane.", "equation": "PTGS2 dimer + ASA- → Ac-PTGS2 dimer + ST", "inputs": [ { "node_id": "R-HSA-140491", "reactome_id": "R-HSA-140491", "label": "PTGS2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-2314693", "reactome_id": "R-ALL-2314693", "label": "ASA-", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2314687", "reactome_id": "R-HSA-2314687", "label": "Ac-PTGS2 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-2314690", "reactome_id": "R-ALL-2314690", "label": "ST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30762", "formula": "C7H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8175750", "title": "Acetylation of human prostaglandin endoperoxide synthase-2 (cyclooxygenase-2) by aspirin", "journal": "J. Biol. Chem.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8175750/" }, { "id": "PMID:21467029", "title": "Human cyclooxygenase-2 is a sequence homodimer that functions as a conformational heterodimer", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21467029/" } ], "x": 590, "y": 1745.7368421052631, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2317530", "label": "SREBP1A,1C,2 binds SCAP:cholesterol:INSIG and is retained in the endoplasmic reticulum", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2317530", "summary": "SREBP1A,1C,2 binds SCAP:cholesterol:INSIG and is retained in the endoplasmic reticulum is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C,2:SCAP, INSIG, CHOL to SREBP1A,1C,2:SCAP:cholesterol:INSIG in endoplasmic reticulum membrane.", "equation": "SREBP1A,1C,2:SCAP + 4 INSIG + 4 CHOL → SREBP1A,1C,2:SCAP:cholesterol:INSIG", "inputs": [ { "node_id": "R-HSA-2317520", "reactome_id": "R-HSA-2317520", "label": "SREBP1A,1C,2:SCAP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1655749", "reactome_id": "R-HSA-1655749", "label": "INSIG", "role": "input", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-193384", "reactome_id": "R-ALL-193384", "label": "CHOL", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655766", "reactome_id": "R-HSA-1655766", "label": "SREBP1A,1C,2:SCAP:cholesterol:INSIG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein sequestering activity of SREBP1A,1C,2:SCAP [endoplasmic reticulum membrane]", "activity_id": "9987239", "ec_numbers": null, "accessions": [] } ], "citations": [], "x": 4730, "y": 1754.0227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2317531", "label": "SREBP1A,1C,2 binds SCAP:INSIG:oxysterol and is retained in the endoplasmic reticulum", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2317531", "summary": "SREBP1A,1C,2 binds SCAP:INSIG:oxysterol and is retained in the endoplasmic reticulum is a Reactome release 97 curated reaction event. The source record connects INSIG:oxysterol, SREBP1A,1C,2:SCAP to SREBP1A,1C,2:SCAP:INSIG:oxysterol in endoplasmic reticulum membrane.", "equation": "4 INSIG:oxysterol + SREBP1A,1C,2:SCAP → SREBP1A,1C,2:SCAP:INSIG:oxysterol", "inputs": [ { "node_id": "R-HSA-2317517", "reactome_id": "R-HSA-2317517", "label": "INSIG:oxysterol", "role": "input", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2317520", "reactome_id": "R-HSA-2317520", "label": "SREBP1A,1C,2:SCAP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2317522", "reactome_id": "R-HSA-2317522", "label": "SREBP1A,1C,2:SCAP:INSIG:oxysterol", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein sequestering activity of SREBP1A,1C,2:SCAP [endoplasmic reticulum membrane]", "activity_id": "9987239", "ec_numbers": null, "accessions": [] } ], "citations": [], "x": 4730, "y": 1818.8560606060605, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2393939", "label": "Cytosolic NUDT5 hydrolyses ADP-ribose to R5P and AMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10413" ], "source_url": "https://reactome.org/content/detail/R-HSA-2393939", "summary": "Cytosolic NUDT5 hydrolyses ADP-ribose to R5P and AMP is a Reactome release 97 curated reaction event. The source record connects ADP-D-ribose, H2O to AMP, R5P, H+ in cytosol and cross-references RHEA:10413.", "equation": "ADP-D-ribose + H2O → AMP + R5P + 2 H+", "inputs": [ { "node_id": "R-ALL-1131972", "reactome_id": "R-ALL-1131972", "label": "ADP-D-ribose", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57967", "formula": "C15H21N5O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ADP-ribose diphosphatase activity of NUDT5 dimer [cytosol]", "activity_id": "2393952", "ec_numbers": [ "3.6.1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:17052728", "title": "Crystal structures of human NUDT5 reveal insights into the structural basis of the substrate specificity", "journal": "J. Mol. Biol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17052728/" }, { "id": "PMID:18462755", "title": "Molecular mechanism of ADP-ribose hydrolysis by human NUDT5 from structural and kinetic studies", "journal": "J. Mol. Biol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18462755/" }, { "id": "PMID:21389046", "title": "Cleavage of oxidized guanine nucleotide and ADP sugar by human NUDT5 protein", "journal": "J. Biochem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21389046/" } ], "x": 2938.666666666667, "y": 3088.7738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2393954", "label": "Mitochondrial NUDT9 hydrolyses ADP-ribose to R5P and AMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:10413" ], "source_url": "https://reactome.org/content/detail/R-HSA-2393954", "summary": "Mitochondrial NUDT9 hydrolyses ADP-ribose to R5P and AMP is a Reactome release 97 curated reaction event. The source record connects ADP-Rib, H2O to AMP, R5P, H+ in mitochondrial matrix and cross-references RHEA:10413.", "equation": "ADP-Rib + H2O → AMP + R5P + 2 H+", "inputs": [ { "node_id": "R-ALL-2393945", "reactome_id": "R-ALL-2393945", "label": "ADP-Rib", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57967", "formula": "C15H21N5O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2393950", "reactome_id": "R-ALL-2393950", "label": "R5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ADP-ribose diphosphatase activity of NUDT9 [mitochondrial matrix]", "activity_id": "2393912", "ec_numbers": [ "3.6.1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:12427752", "title": "NUDT9, a member of the Nudix hydrolase family, is an evolutionarily conserved mitochondrial ADP-ribose pyrophosphatase", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12427752/" }, { "id": "PMID:12948489", "title": "The crystal structure and mutational analysis of human NUDT9", "journal": "J. Mol. Biol.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12948489/" } ], "x": 2381.3333333333335, "y": 3095.8928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395512", "label": "FDXR transfers electrons to FDX1,2 (FDX1L)", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "iron-sulfur" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2395512", "summary": "FDXR transfers electrons to FDX1,2 (FDX1L) is a Reactome release 97 curated reaction event. The source record connects FDXR:FADH2, FDX1,FDX2 (oxidized) to FDXR:FAD, H+, FDX1,FDX2 (reduced) in mitochondrial matrix.", "equation": "FDXR:FADH2 + 2 FDX1,FDX2 (oxidized) → FDXR:FAD + 2 H+ + 2 FDX1,FDX2 (reduced)", "inputs": [ { "node_id": "R-HSA-2395502", "reactome_id": "R-HSA-2395502", "label": "FDXR:FADH2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2408370", "reactome_id": "R-HSA-2408370", "label": "FDX1,FDX2 (oxidized)", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2395508", "reactome_id": "R-HSA-2395508", "label": "FDXR:FAD", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2408372", "reactome_id": "R-HSA-2408372", "label": "FDX1,FDX2 (reduced)", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "ferredoxin-NADP+ reductase activity of FDXR [mitochondrial matrix]", "activity_id": "9954152", "ec_numbers": [ "1.18.1.2" ], "accessions": [ "P22570" ] } ], "citations": [ { "id": "PMID:22101253", "title": "Both human ferredoxins 1 and 2 and ferredoxin reductase are important for iron-sulfur cluster biogenesis", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22101253/" }, { "id": "PMID:20547883", "title": "Humans possess two mitochondrial ferredoxins, Fdx1 and Fdx2, with distinct roles in steroidogenesis, heme, and Fe/S cluster biosynthesis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20547883/" } ], "x": 5391.333333333333, "y": 3573.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395517", "label": "NADPH transfers electrons to FDXR", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "iron-sulfur" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2395517", "summary": "NADPH transfers electrons to FDXR is a Reactome release 97 curated reaction event. The source record connects NADPH, FDXR:FAD, H+ to NADP+, FDXR:FADH2 in mitochondrial matrix.", "equation": "NADPH + FDXR:FAD + H+ → NADP+ + FDXR:FADH2", "inputs": [ { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2395508", "reactome_id": "R-HSA-2395508", "label": "FDXR:FAD", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2395502", "reactome_id": "R-HSA-2395502", "label": "FDXR:FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "ferredoxin-NADP+ reductase activity of FDXR [mitochondrial matrix]", "activity_id": "9954152", "ec_numbers": [ "1.18.1.2" ], "accessions": [ "P22570" ] } ], "citations": [ { "id": "PMID:1567230", "title": "Expression and characterization of human mitochondrial ferredoxin reductase in Escherichia coli", "journal": "Arch. Biochem. Biophys.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1567230/" } ], "x": 5670, "y": 3379.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395818", "label": "NUDT1 hydrolyses 2-oxo-dATP to 2-oxo-dAMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:31584" ], "source_url": "https://reactome.org/content/detail/R-HSA-2395818", "summary": "NUDT1 hydrolyses 2-oxo-dATP to 2-oxo-dAMP is a Reactome release 97 curated reaction event. The source record connects H2O, 2-oxo-dATP(4-) to 2-oxo-dAMP(2-), H+, PPi in cytosol and cross-references RHEA:31584.", "equation": "H2O + 2-oxo-dATP(4-) → 2-oxo-dAMP(2-) + H+ + PPi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731234", "reactome_id": "R-ALL-9731234", "label": "2-oxo-dATP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77897", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9731227", "reactome_id": "R-ALL-9731227", "label": "2-oxo-dAMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63212", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "2-hydroxy-dATP hydrolase activity of NUDT1 [cytosol]", "activity_id": "2532794", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10373420", "title": "The oxidized forms of dATP are substrates for the human MutT homologue, the hMTH1 protein", "journal": "J. Biol. Chem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10373420/" }, { "id": "PMID:7782328", "title": "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria", "journal": "J. Biol. Chem.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7782328/" }, { "id": "PMID:15133035", "title": "Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15133035/" }, { "id": "PMID:10536140", "title": "Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism", "journal": "Nucleic Acids Res.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10536140/" }, { "id": "PMID:11756418", "title": "A molecular basis for the selective recognition of 2-hydroxy-dATP and 8-oxo-dGTP by human MTH1", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11756418/" }, { "id": "PMID:12857738", "title": "An oxidized purine nucleoside triphosphatase, MTH1, suppresses cell death caused by oxidative stress", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12857738/" } ], "x": 2660, "y": 3095.8928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395849", "label": "NUDT1 hydrolyses 8-oxo-dGTP to 8-oxo-dGMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:31576" ], "source_url": "https://reactome.org/content/detail/R-HSA-2395849", "summary": "NUDT1 hydrolyses 8-oxo-dGTP to 8-oxo-dGMP is a Reactome release 97 curated reaction event. The source record connects H2O, 8-oxo-dGTP(4-) to 8-oxo-dGMP(2-), H+, PPi in cytosol and cross-references RHEA:31576.", "equation": "H2O + 8-oxo-dGTP(4-) → 8-oxo-dGMP(2-) + H+ + PPi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731302", "reactome_id": "R-ALL-9731302", "label": "8-oxo-dGTP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77896", "formula": "C10H12N5O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9731296", "reactome_id": "R-ALL-9731296", "label": "8-oxo-dGMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63224", "formula": "C10H12N5O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity of NUDT1 [cytosol]", "activity_id": "2395845", "ec_numbers": [ "3.6.1.55" ], "accessions": [] } ], "citations": [ { "id": "PMID:7782328", "title": "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria", "journal": "J. Biol. Chem.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7782328/" }, { "id": "PMID:15133035", "title": "Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15133035/" }, { "id": "PMID:10536140", "title": "Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism", "journal": "Nucleic Acids Res.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10536140/" }, { "id": "PMID:8226881", "title": "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis", "journal": "J. Biol. Chem.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8226881/" }, { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" }, { "id": "PMID:12857738", "title": "An oxidized purine nucleoside triphosphatase, MTH1, suppresses cell death caused by oxidative stress", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12857738/" } ], "x": 2938.666666666667, "y": 3095.8928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395869", "label": "NUDT15 hydrolyses 8-oxo-dGTP to 8-oxo-dGMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:31576" ], "source_url": "https://reactome.org/content/detail/R-HSA-2395869", "summary": "NUDT15 hydrolyses 8-oxo-dGTP to 8-oxo-dGMP is a Reactome release 97 curated reaction event. The source record connects H2O, 8-oxo-dGTP(4-) to 8-oxo-dGMP(2-), H+, PPi in cytosol and cross-references RHEA:31576.", "equation": "H2O + 8-oxo-dGTP(4-) → 8-oxo-dGMP(2-) + H+ + PPi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731302", "reactome_id": "R-ALL-9731302", "label": "8-oxo-dGTP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77896", "formula": "C10H12N5O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9731296", "reactome_id": "R-ALL-9731296", "label": "8-oxo-dGMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63224", "formula": "C10H12N5O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "8-oxo-7,8-dihydrodeoxyguanosine triphosphate pyrophosphatase activity of NUDT15 [cytosol]", "activity_id": "2395882", "ec_numbers": [ "3.6.1.55" ], "accessions": [ "Q9NV35" ] } ], "citations": [ { "id": "PMID:12767940", "title": "Mouse MTH2 protein which prevents mutations caused by 8-oxoguanine nucleotides", "journal": "Biochem. Biophys. Res. Commun.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12767940/" }, { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" } ], "x": 2381.3333333333335, "y": 3103.0119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395872", "label": "NUDT1 hydrolyses 2-oxo-ATP to 2-oxo-AMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:67393" ], "source_url": "https://reactome.org/content/detail/R-HSA-2395872", "summary": "NUDT1 hydrolyses 2-oxo-ATP to 2-oxo-AMP is a Reactome release 97 curated reaction event. The source record connects H2O, 2-oxo-ATP(4-) to 2-oxo-AMP(2-), H+, PPi in cytosol and cross-references RHEA:67393.", "equation": "H2O + 2-oxo-ATP(4-) → 2-oxo-AMP(2-) + H+ + PPi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731240", "reactome_id": "R-ALL-9731240", "label": "2-oxo-ATP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:172878", "formula": "C10H12N5O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9731231", "reactome_id": "R-ALL-9731231", "label": "2-oxo-AMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:71395", "formula": "C10H12N5O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "2-hydroxy-ATP hydrolase activity of NUDT1 [cytosol]", "activity_id": "2395832", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11139615", "title": "Human MTH1 protein hydrolyzes the oxidized ribonucleotide, 2-hydroxy-ATP", "journal": "Nucleic Acids Res.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11139615/" }, { "id": "PMID:7782328", "title": "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria", "journal": "J. Biol. Chem.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7782328/" }, { "id": "PMID:15133035", "title": "Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15133035/" } ], "x": 2660, "y": 3103.0119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395873", "label": "NUDT18 hydrolyses 8-oxo-GDP to 8-oxo-GMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:62357" ], "source_url": "https://reactome.org/content/detail/R-HSA-2395873", "summary": "NUDT18 hydrolyses 8-oxo-GDP to 8-oxo-GMP is a Reactome release 97 curated reaction event. The source record connects H2O, 8-oxo-GDP(3-) to Pi, 8-oxo-GMP(2-), H+ in cytosol and cross-references RHEA:62357.", "equation": "H2O + 8-oxo-GDP(3-) → Pi + 8-oxo-GMP(2-) + H+", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731304", "reactome_id": "R-ALL-9731304", "label": "8-oxo-GDP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:143554", "formula": "C10H12N5O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9731298", "reactome_id": "R-ALL-9731298", "label": "8-oxo-GMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:145694", "formula": "C10H12N5O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "8-oxo-GDP phosphatase activity of NUDT18-1 [cytosol]", "activity_id": "2532781", "ec_numbers": [ "3.6.1.58" ], "accessions": [ "Q6ZVK8" ] } ], "citations": [ { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" } ], "x": 2938.666666666667, "y": 3103.0119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395876", "label": "NUDT15 hydrolyses 8-oxo-dGDP to 8-oxo-dGMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:32064" ], "source_url": "https://reactome.org/content/detail/R-HSA-2395876", "summary": "NUDT15 hydrolyses 8-oxo-dGDP to 8-oxo-dGMP is a Reactome release 97 curated reaction event. The source record connects H2O, 8-oxo-dGDP(3-) to Pi, 8-oxo-dGMP(2-), H+ in cytosol and cross-references RHEA:32064.", "equation": "H2O + 8-oxo-dGDP(3-) → Pi + 8-oxo-dGMP(2-) + H+", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731300", "reactome_id": "R-ALL-9731300", "label": "8-oxo-dGDP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63715", "formula": "C10H12N5O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9731296", "reactome_id": "R-ALL-9731296", "label": "8-oxo-dGMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63224", "formula": "C10H12N5O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "8-oxo-dGDP phosphatase activity of NUDT15 [cytosol]", "activity_id": "2395875", "ec_numbers": [ "3.6.1.58" ], "accessions": [ "Q9NV35" ] } ], "citations": [ { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" } ], "x": 2381.3333333333335, "y": 3110.1309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395879", "label": "NUDT18 hydrolyses 8-oxo-dGDP to 8-oxo-dGMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:32064" ], "source_url": "https://reactome.org/content/detail/R-HSA-2395879", "summary": "NUDT18 hydrolyses 8-oxo-dGDP to 8-oxo-dGMP is a Reactome release 97 curated reaction event. The source record connects H2O, 8-oxo-dGDP(3-) to Pi, 8-oxo-dGMP(2-), H+ in cytosol and cross-references RHEA:32064.", "equation": "H2O + 8-oxo-dGDP(3-) → Pi + 8-oxo-dGMP(2-) + H+", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731300", "reactome_id": "R-ALL-9731300", "label": "8-oxo-dGDP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63715", "formula": "C10H12N5O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9731296", "reactome_id": "R-ALL-9731296", "label": "8-oxo-dGMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63224", "formula": "C10H12N5O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "8-oxo-dGDP phosphatase activity of NUDT18-1 [cytosol]", "activity_id": "2395887", "ec_numbers": [ "3.6.1.58" ], "accessions": [ "Q6ZVK8" ] } ], "citations": [ { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" } ], "x": 2660, "y": 3110.1309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2395965", "label": "NUDT18 hydrolyses 8-oxo-dADP to 8-oxo-dAMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:35220" ], "source_url": "https://reactome.org/content/detail/R-HSA-2395965", "summary": "NUDT18 hydrolyses 8-oxo-dADP to 8-oxo-dAMP is a Reactome release 97 curated reaction event. The source record connects H2O, 8-oxo-dADP(3-) to Pi, 8-oxo-dAMP(2-), H+ in cytosol and cross-references RHEA:35220.", "equation": "H2O + 8-oxo-dADP(3-) → Pi + 8-oxo-dAMP(2-) + H+", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731226", "reactome_id": "R-ALL-9731226", "label": "8-oxo-dADP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:71362", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9731235", "reactome_id": "R-ALL-9731235", "label": "8-oxo-dAMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:71361", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "8-hydroxy-dADP phosphatase activity of NUDT18-1 [cytosol]", "activity_id": "2532779", "ec_numbers": null, "accessions": [ "Q6ZVK8" ] } ], "citations": [ { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" } ], "x": 2938.666666666667, "y": 3110.1309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426144", "label": "SREBP1A,2 binds the MVD promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426144", "summary": "SREBP1A,2 binds the MVD promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, MVD gene to SREBP1A,2:MVD gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + MVD gene → SREBP1A,2:MVD gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393981", "reactome_id": "R-HSA-2393981", "label": "MVD gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426130", "reactome_id": "R-HSA-2426130", "label": "SREBP1A,2:MVD gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2 dimer [nucleoplasm]", "activity_id": "9987247", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" } ], "x": 5008.666666666667, "y": 1765.810606060606, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426146", "label": "SREBP1A,1C,2 binds the FDFT1 promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426146", "summary": "SREBP1A,1C,2 binds the FDFT1 promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C,2 dimer, NF-Y, SP1 to SREBP1A,1C,2:NF-Y:SP1:FDFT1 gene in nucleoplasm.", "equation": "SREBP1A,1C,2 dimer + NF-Y + SP1 + FDFT1 gene → SREBP1A,1C,2:NF-Y:SP1:FDFT1 gene", "inputs": [ { "node_id": "R-HSA-1655744", "reactome_id": "R-HSA-1655744", "label": "SREBP1A,1C,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2393991", "reactome_id": "R-HSA-2393991", "label": "FDFT1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426135", "reactome_id": "R-HSA-2426135", "label": "SREBP1A,1C,2:NF-Y:SP1:FDFT1 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,1C,2,NF-Y,SP1 [nucleoplasm]", "activity_id": "9987317", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9604010", "title": "Multiple DNA elements for sterol regulatory element-binding protein and NF-Y are responsible for sterol-regulated transcription of the genes for human 3-hydroxy-3-methylglutaryl coenzyme A synthase and squalene synthase", "journal": "J Biochem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9604010/" }, { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" }, { "id": "PMID:9748295", "title": "Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9748295/" } ], "x": 4451.333333333333, "y": 1771.7045454545455, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426147", "label": "SREBP1A,1C,2 binds the FDPS promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426147", "summary": "SREBP1A,1C,2 binds the FDPS promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C,2 dimer, NF-Y, FDPS gene to SREBP1A,1C,2:NF-Y:FDPS gene in nucleoplasm.", "equation": "SREBP1A,1C,2 dimer + NF-Y + FDPS gene → SREBP1A,1C,2:NF-Y:FDPS gene", "inputs": [ { "node_id": "R-HSA-1655744", "reactome_id": "R-HSA-1655744", "label": "SREBP1A,1C,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393989", "reactome_id": "R-HSA-2393989", "label": "FDPS gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426134", "reactome_id": "R-HSA-2426134", "label": "SREBP1A,1C,2:NF-Y:FDPS gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of NF-Y,SREBP1A,1C,2 [nucleoplasm]", "activity_id": "9987240", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:20450493", "title": "Sterol-regulatory-element-binding protein 2 and nuclear factor Y control human farnesyl diphosphate synthase expression and affect cell proliferation in hepatoblastoma cells", "journal": "Biochem J", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20450493/" }, { "id": "PMID:8798690", "title": "Synergistic binding of sterol regulatory element-binding protein and NF-Y to the farnesyl diphosphate synthase promoter is critical for sterol-regulated expression of the gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8798690/" }, { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" }, { "id": "PMID:12177166", "title": "Transcriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterogenic genes", "journal": "J Lipid Res", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12177166/" }, { "id": "PMID:9748295", "title": "Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9748295/" } ], "x": 4730, "y": 1771.7045454545455, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426148", "label": "SREBP1A,1C binds the FASN promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426148", "summary": "SREBP1A,1C binds the FASN promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C dimer, NF-Y, SP1 to SREBP1A,1C:NF-Y:SP1:FASN gene in nucleoplasm.", "equation": "SREBP1A,1C dimer + NF-Y + SP1 + FASN gene → SREBP1A,1C:NF-Y:SP1:FASN gene", "inputs": [ { "node_id": "R-HSA-2065537", "reactome_id": "R-HSA-2065537", "label": "SREBP1A,1C dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2393979", "reactome_id": "R-HSA-2393979", "label": "FASN gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655738", "reactome_id": "R-HSA-1655738", "label": "SREBP1A,1C:NF-Y:SP1:FASN gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,1C,NF-Y,SP1 [nucleoplasm]", "activity_id": "9987230", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:7592729", "title": "Sterol regulation of fatty acid synthase promoter. Coordinate feedback regulation of two major lipid pathways", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7592729/" }, { "id": "PMID:18682402", "title": "Proto-oncogene FBI-1 (Pokemon) and SREBP-1 synergistically activate transcription of fatty-acid synthase gene (FASN)", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18682402/" }, { "id": "PMID:10759542", "title": "Sterol regulation of human fatty acid synthase promoter I requires nuclear factor-Y- and Sp-1-binding sites", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10759542/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" }, { "id": "PMID:12177166", "title": "Transcriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterogenic genes", "journal": "J Lipid Res", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12177166/" }, { "id": "PMID:9748295", "title": "Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9748295/" } ], "x": 5008.666666666667, "y": 1771.7045454545455, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426149", "label": "SREBP1A,1C,2 binds the ELOVL6 promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426149", "summary": "SREBP1A,1C,2 binds the ELOVL6 promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C,2 dimer, ELOVL6 gene to SREBP1A,1C,2:ELOVL6 gene in nucleoplasm.", "equation": "SREBP1A,1C,2 dimer + ELOVL6 gene → SREBP1A,1C,2:ELOVL6 gene", "inputs": [ { "node_id": "R-HSA-1655744", "reactome_id": "R-HSA-1655744", "label": "SREBP1A,1C,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393996", "reactome_id": "R-HSA-2393996", "label": "ELOVL6 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655762", "reactome_id": "R-HSA-1655762", "label": "SREBP1A,1C,2:ELOVL6 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,1C,2 dimer [nucleoplasm]", "activity_id": "9987316", "ec_numbers": null, "accessions": [] } ], "citations": [], "x": 5008.666666666667, "y": 1818.8560606060605, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426150", "label": "SREBP1A,1C,2 binds the HMGCS1 promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426150", "summary": "SREBP1A,1C,2 binds the HMGCS1 promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C,2 dimer, NF-Y, HMGCS1 gene to SREBP1A,1C,2:NF-Y:HMGCS1 gene in nucleoplasm.", "equation": "SREBP1A,1C,2 dimer + NF-Y + HMGCS1 gene → SREBP1A,1C,2:NF-Y:HMGCS1 gene", "inputs": [ { "node_id": "R-HSA-1655744", "reactome_id": "R-HSA-1655744", "label": "SREBP1A,1C,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393972", "reactome_id": "R-HSA-2393972", "label": "HMGCS1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426129", "reactome_id": "R-HSA-2426129", "label": "SREBP1A,1C,2:NF-Y:HMGCS1 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of NF-Y,SREBP1A,1C,2 [nucleoplasm]", "activity_id": "9987240", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9604010", "title": "Multiple DNA elements for sterol regulatory element-binding protein and NF-Y are responsible for sterol-regulated transcription of the genes for human 3-hydroxy-3-methylglutaryl coenzyme A synthase and squalene synthase", "journal": "J Biochem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9604010/" }, { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" }, { "id": "PMID:12177166", "title": "Transcriptional activities of nuclear SREBP-1a, -1c, and -2 to different target promoters of lipogenic and cholesterogenic genes", "journal": "J Lipid Res", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12177166/" }, { "id": "PMID:9748295", "title": "Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9748295/" } ], "x": 4451.333333333333, "y": 1777.5984848484848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426151", "label": "SREBP1A,2 binds the SQLE promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426151", "summary": "SREBP1A,2 binds the SQLE promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, SQLE gene, NF-Y to SREBP1A,2:SQLE gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + SQLE gene + NF-Y → SREBP1A,2:SQLE gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393973", "reactome_id": "R-HSA-2393973", "label": "SQLE gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426136", "reactome_id": "R-HSA-2426136", "label": "SREBP1A,2:SQLE gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,NF-Y [nucleoplasm]", "activity_id": "9987321", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12083769", "title": "SREBP-2 and NF-Y are involved in the transcriptional regulation of squalene epoxidase", "journal": "Biochem Biophys Res Commun", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12083769/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" } ], "x": 4730, "y": 1777.5984848484848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426152", "label": "SREBP1A,2 binds the IDI1 promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426152", "summary": "SREBP1A,2 binds the IDI1 promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, NF-Y, SP1 to SREBP1A,2:NF-Y:SP1:IDI1 gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + NF-Y + SP1 + IDI1 gene → SREBP1A,2:NF-Y:SP1:IDI1 gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2393982", "reactome_id": "R-HSA-2393982", "label": "IDI1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426138", "reactome_id": "R-HSA-2426138", "label": "SREBP1A,2:NF-Y:SP1:IDI1 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,NF-Y,SP1 [nucleoplasm]", "activity_id": "9987232", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" } ], "x": 5008.666666666667, "y": 1777.5984848484848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426153", "label": "SREBP1A,1C binds the ACACB promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426153", "summary": "SREBP1A,1C binds the ACACB promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C dimer, ACACB gene, SP1 to SREBP1A,1C:ACACB gene in nucleoplasm.", "equation": "SREBP1A,1C dimer + ACACB gene + SP1 → SREBP1A,1C:ACACB gene", "inputs": [ { "node_id": "R-HSA-2065537", "reactome_id": "R-HSA-2065537", "label": "SREBP1A,1C dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393980", "reactome_id": "R-HSA-2393980", "label": "ACACB gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426125", "reactome_id": "R-HSA-2426125", "label": "SREBP1A,1C:ACACB gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SP1,SREBP1A,1C [nucleoplasm]", "activity_id": "9987255", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12764144", "title": "Acetyl-CoA carboxylase beta gene is regulated by sterol regulatory element-binding protein-1 in liver", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12764144/" } ], "x": 4451.333333333333, "y": 1783.4924242424242, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426154", "label": "SREBP1A,2 binds the LSS promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426154", "summary": "SREBP1A,2 binds the LSS promoter is a Reactome release 97 curated reaction event. The source record connects LSS gene, SREBP1A,2 dimer to SREBP1A,2:LSS gene in nucleoplasm.", "equation": "LSS gene + SREBP1A,2 dimer → SREBP1A,2:LSS gene", "inputs": [ { "node_id": "R-HSA-2393967", "reactome_id": "R-HSA-2393967", "label": "LSS gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426137", "reactome_id": "R-HSA-2426137", "label": "SREBP1A,2:LSS gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2 dimer [nucleoplasm]", "activity_id": "9987247", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" }, { "id": "PMID:9748295", "title": "Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9748295/" } ], "x": 4730, "y": 1783.4924242424242, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426155", "label": "SREBP1A,2 binds the DHCR7 promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426155", "summary": "SREBP1A,2 binds the DHCR7 promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, NF-Y, SP1 to SREBP1A,2:NF-Y:SP1:DHCR7 gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + NF-Y + SP1 + DHCR7 gene → SREBP1A,2:NF-Y:SP1:DHCR7 gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2393971", "reactome_id": "R-HSA-2393971", "label": "DHCR7 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426124", "reactome_id": "R-HSA-2426124", "label": "SREBP1A,2:NF-Y:SP1:DHCR7 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,NF-Y,SP1 [nucleoplasm]", "activity_id": "9987232", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" } ], "x": 5008.666666666667, "y": 1783.4924242424242, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426156", "label": "SREBP1A,2 binds the PMVK promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426156", "summary": "SREBP1A,2 binds the PMVK promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, NF-Y, SP1 to SREBP1A,2:NF-Y:SP1:PMVK gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + NF-Y + SP1 + PMVK gene → SREBP1A,2:NF-Y:SP1:PMVK gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2393994", "reactome_id": "R-HSA-2393994", "label": "PMVK gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426139", "reactome_id": "R-HSA-2426139", "label": "SREBP1A,2:NF-Y:SP1:PMVK gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,NF-Y,SP1 [nucleoplasm]", "activity_id": "9987232", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" } ], "x": 4451.333333333333, "y": 1789.3863636363635, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426157", "label": "SREBP1A,2 binds the MVK promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426157", "summary": "SREBP1A,2 binds the MVK promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, NF-Y, SP1 to SREBP1A,2:NF-Y:SP1:MVK gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + NF-Y + SP1 + MVK gene → SREBP1A,2:NF-Y:SP1:MVK gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2393995", "reactome_id": "R-HSA-2393995", "label": "MVK gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426127", "reactome_id": "R-HSA-2426127", "label": "SREBP1A,2:NF-Y:SP1:MVK gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,NF-Y,SP1 [nucleoplasm]", "activity_id": "9987232", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" } ], "x": 4730, "y": 1789.3863636363635, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426158", "label": "SREBP1A,1C binds the GPAM promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426158", "summary": "SREBP1A,1C binds the GPAM promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C dimer, GPAM gene, NF-Y to SREBP1A,1C:NF-Y:GPAM gene in nucleoplasm.", "equation": "SREBP1A,1C dimer + GPAM gene + NF-Y → SREBP1A,1C:NF-Y:GPAM gene", "inputs": [ { "node_id": "R-HSA-2065537", "reactome_id": "R-HSA-2065537", "label": "SREBP1A,1C dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393968", "reactome_id": "R-HSA-2393968", "label": "GPAM gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426131", "reactome_id": "R-HSA-2426131", "label": "SREBP1A,1C:NF-Y:GPAM gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,1C,NF-Y [nucleoplasm]", "activity_id": "9987233", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:22634312", "title": "Identification and functional characterization of human glycerol-3-phosphate acyltransferase 1 gene promoters", "journal": "Biochem. Biophys. Res. Commun.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22634312/" } ], "x": 5008.666666666667, "y": 1789.3863636363635, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426160", "label": "SREBP1A,1C binds the ACACA promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426160", "summary": "SREBP1A,1C binds the ACACA promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,1C dimer, ACACA gene to SREBP1A,1C:ACACA gene in nucleoplasm.", "equation": "SREBP1A,1C dimer + ACACA gene → SREBP1A,1C:ACACA gene", "inputs": [ { "node_id": "R-HSA-2065537", "reactome_id": "R-HSA-2065537", "label": "SREBP1A,1C dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393997", "reactome_id": "R-HSA-2393997", "label": "ACACA gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426141", "reactome_id": "R-HSA-2426141", "label": "SREBP1A,1C:ACACA gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,1C dimer [nucleoplasm]", "activity_id": "9987248", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9300785", "title": "Sterol regulation of acetyl coenzyme A carboxylase promoter requires two interdependent binding sites for sterol regulatory element binding proteins", "journal": "J Lipid Res", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9300785/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" } ], "x": 4451.333333333333, "y": 1795.280303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426161", "label": "SREBP1A,2 binds the GGPS1 promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426161", "summary": "SREBP1A,2 binds the GGPS1 promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, NF-Y, SP1 to SREBP1A,2:NF-Y:SP1:GGPS1 gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + NF-Y + SP1 + GGPS1 gene → SREBP1A,2:NF-Y:SP1:GGPS1 gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2393998", "reactome_id": "R-HSA-2393998", "label": "GGPS1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426126", "reactome_id": "R-HSA-2426126", "label": "SREBP1A,2:NF-Y:SP1:GGPS1 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,NF-Y,SP1 [nucleoplasm]", "activity_id": "9987232", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" } ], "x": 4730, "y": 1795.280303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426162", "label": "SREBP1A,2 binds the HMGCR promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426162", "summary": "SREBP1A,2 binds the HMGCR promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, NF-Y, HMGCR gene to SREBP1A,2:NF-Y:HMGCR gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + NF-Y + HMGCR gene → SREBP1A,2:NF-Y:HMGCR gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2393990", "reactome_id": "R-HSA-2393990", "label": "HMGCR gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426128", "reactome_id": "R-HSA-2426128", "label": "SREBP1A,2:NF-Y:HMGCR gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,SP1 [nucleoplasm]", "activity_id": "9987229", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8647822", "title": "A direct role for sterol regulatory element binding protein in activation of 3-hydroxy-3-methylglutaryl coenzyme A reductase gene", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8647822/" }, { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" }, { "id": "PMID:9748295", "title": "Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9748295/" } ], "x": 5008.666666666667, "y": 1795.280303030303, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426163", "label": "SREBP1A,2 binds the CYP51A1 promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426163", "summary": "SREBP1A,2 binds the CYP51A1 promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, NF-Y, SP1 to SREBP1A,2:NF-Y:SP1:CYP51A1 gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + NF-Y + SP1 + CYP51A1 gene → SREBP1A,2:NF-Y:SP1:CYP51A1 gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1592223", "reactome_id": "R-HSA-1592223", "label": "SP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5660427", "reactome_id": "R-HSA-5660427", "label": "CYP51A1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426132", "reactome_id": "R-HSA-2426132", "label": "SREBP1A,2:NF-Y:SP1:CYP51A1 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,NF-Y,SP1 [nucleoplasm]", "activity_id": "9987232", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" }, { "id": "PMID:9748295", "title": "Differential stimulation of cholesterol and unsaturated fatty acid biosynthesis in cells expressing individual nuclear sterol regulatory element-binding proteins", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9748295/" } ], "x": 4451.333333333333, "y": 1801.1742424242425, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2426164", "label": "SREBP1A,2 binds the SC5DL promoter", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2426164", "summary": "SREBP1A,2 binds the SC5DL promoter is a Reactome release 97 curated reaction event. The source record connects SREBP1A,2 dimer, NF-Y, SC5DL gene to SREBP1A,2:NF-Y:SC5DL gene in nucleoplasm.", "equation": "SREBP1A,2 dimer + NF-Y + SC5DL gene → SREBP1A,2:NF-Y:SC5DL gene", "inputs": [ { "node_id": "R-HSA-2065551", "reactome_id": "R-HSA-2065551", "label": "SREBP1A,2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-381204", "reactome_id": "R-HSA-381204", "label": "NF-Y", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2394001", "reactome_id": "R-HSA-2394001", "label": "SC5DL gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2426140", "reactome_id": "R-HSA-2426140", "label": "SREBP1A,2:NF-Y:SC5DL gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,2,SP1 [nucleoplasm]", "activity_id": "9987229", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" }, { "id": "PMID:18559965", "title": "Microarray analyses of SREBP-1a and SREBP-1c target genes identify new regulatory pathways in muscle", "journal": "Physiol Genomics", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18559965/" } ], "x": 4730, "y": 1801.1742424242425, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2509793", "label": "NUDT16 hydrolyses dIDP to dIMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:35212" ], "source_url": "https://reactome.org/content/detail/R-HSA-2509793", "summary": "NUDT16 hydrolyses dIDP to dIMP is a Reactome release 97 curated reaction event. The source record connects dIDP, H2O to dIMP, Pi, H+ in nucleoplasm and cross-references RHEA:35212.", "equation": "dIDP + H2O → dIMP + Pi + H+", "inputs": [ { "node_id": "R-ALL-2509796", "reactome_id": "R-ALL-2509796", "label": "dIDP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62286", "formula": "C10H11N4O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113518", "reactome_id": "R-ALL-113518", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2509805", "reactome_id": "R-ALL-2509805", "label": "dIMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61194", "formula": "C10H11N4O7P", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113550", "reactome_id": "R-ALL-113550", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dIDP phosphatase activity of NUDT16 dimer [nucleoplasm]", "activity_id": "2509802", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:20385596", "title": "NUDT16 is a (deoxy)inosine diphosphatase, and its deficiency induces accumulation of single-strand breaks in nuclear DNA and growth arrest", "journal": "Nucleic Acids Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20385596/" }, { "id": "PMID:17567574", "title": "Metal determines efficiency and substrate specificity of the nuclear NUDIX decapping proteins X29 and H29K (Nudt16)", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17567574/" } ], "x": 2381.3333333333335, "y": 3117.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2509816", "label": "NUDT16 hydrolyses IDP to IMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:35208" ], "source_url": "https://reactome.org/content/detail/R-HSA-2509816", "summary": "NUDT16 hydrolyses IDP to IMP is a Reactome release 97 curated reaction event. The source record connects IDP, H2O to IMP, Pi, H+ in nucleoplasm and cross-references RHEA:35208.", "equation": "IDP + H2O → IMP + Pi + H+", "inputs": [ { "node_id": "R-ALL-2509811", "reactome_id": "R-ALL-2509811", "label": "IDP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58280", "formula": "C10H11N4O11P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113518", "reactome_id": "R-ALL-113518", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2509792", "reactome_id": "R-ALL-2509792", "label": "IMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58053", "formula": "C10H11N4O8P", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113550", "reactome_id": "R-ALL-113550", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "IDP phosphatase activity of NUDT16 dimer [nucleoplasm]", "activity_id": "2509812", "ec_numbers": [ "3.6.1.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:20385596", "title": "NUDT16 is a (deoxy)inosine diphosphatase, and its deficiency induces accumulation of single-strand breaks in nuclear DNA and growth arrest", "journal": "Nucleic Acids Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20385596/" }, { "id": "PMID:17567574", "title": "Metal determines efficiency and substrate specificity of the nuclear NUDIX decapping proteins X29 and H29K (Nudt16)", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17567574/" } ], "x": 2660, "y": 3117.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2509827", "label": "ITPA hydrolyses ITP to IMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:29400", "RHEA:28611" ], "source_url": "https://reactome.org/content/detail/R-HSA-2509827", "summary": "ITPA hydrolyses ITP to IMP is a Reactome release 97 curated reaction event. The source record connects ITP, H2O to IMP, PPi, H+ in cytosol and cross-references RHEA:29400, RHEA:28611.", "equation": "ITP + H2O → IMP + PPi + H+", "inputs": [ { "node_id": "R-ALL-2509836", "reactome_id": "R-ALL-2509836", "label": "ITP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61402", "formula": "C10H11N4O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29602", "reactome_id": "R-ALL-29602", "label": "IMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58053", "formula": "C10H11N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ITP diphosphatase activity of ITPA dimer [cytosol]", "activity_id": "2509830", "ec_numbers": [ "3.6.1.66" ], "accessions": [] } ], "citations": [ { "id": "PMID:20081199", "title": "NUDT16 and ITPA play a dual protective role in maintaining chromosome stability and cell growth by eliminating dIDP/IDP and dITP/ITP from nucleotide pools in mammals", "journal": "Nucleic Acids Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20081199/" }, { "id": "PMID:11278832", "title": "Cloning, expression, and characterization of a human inosine triphosphate pyrophosphatase encoded by the itpa gene", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11278832/" } ], "x": 2938.666666666667, "y": 3117.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2509831", "label": "ITPA hydrolyses XTP to XMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:28611" ], "source_url": "https://reactome.org/content/detail/R-HSA-2509831", "summary": "ITPA hydrolyses XTP to XMP is a Reactome release 97 curated reaction event. The source record connects XTP, H2O to XMP, PPi, H+ in cytosol and cross-references RHEA:28611.", "equation": "XTP + H2O → XMP + PPi + H+", "inputs": [ { "node_id": "R-ALL-2509848", "reactome_id": "R-ALL-2509848", "label": "XTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61314", "formula": "C10H11N4O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111584", "reactome_id": "R-ALL-111584", "label": "XMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57464", "formula": "C10H11N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "XTP diphosphatase activity of ITPA dimer [cytosol]", "activity_id": "2509849", "ec_numbers": [ "3.6.1.66" ], "accessions": [] } ], "citations": [ { "id": "PMID:20081199", "title": "NUDT16 and ITPA play a dual protective role in maintaining chromosome stability and cell growth by eliminating dIDP/IDP and dITP/ITP from nucleotide pools in mammals", "journal": "Nucleic Acids Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20081199/" }, { "id": "PMID:11278832", "title": "Cloning, expression, and characterization of a human inosine triphosphate pyrophosphatase encoded by the itpa gene", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11278832/" } ], "x": 2381.3333333333335, "y": 3181.3214285714284, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2509838", "label": "ITPA hydrolyses dITP to dIMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:28343", "RHEA:28434" ], "source_url": "https://reactome.org/content/detail/R-HSA-2509838", "summary": "ITPA hydrolyses dITP to dIMP is a Reactome release 97 curated reaction event. The source record connects dITP, H2O to PPi, dIMP, H+ in cytosol and cross-references RHEA:28343, RHEA:28434.", "equation": "dITP + H2O → PPi + dIMP + H+", "inputs": [ { "node_id": "R-ALL-2509835", "reactome_id": "R-ALL-2509835", "label": "dITP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61382", "formula": "C10H11N4O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9731659", "reactome_id": "R-ALL-9731659", "label": "dIMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61194", "formula": "C10H11N4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dITP diphosphatase activity of ITPA dimer [cytosol]", "activity_id": "2509845", "ec_numbers": [ "3.6.1.66" ], "accessions": [] } ], "citations": [ { "id": "PMID:20081199", "title": "NUDT16 and ITPA play a dual protective role in maintaining chromosome stability and cell growth by eliminating dIDP/IDP and dITP/ITP from nucleotide pools in mammals", "journal": "Nucleic Acids Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20081199/" }, { "id": "PMID:11278832", "title": "Cloning, expression, and characterization of a human inosine triphosphate pyrophosphatase encoded by the itpa gene", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11278832/" } ], "x": 2381.3333333333335, "y": 3124.3690476190477, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-264622", "label": "Cho is acetylated to AcCho by CHAT", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18822" ], "source_url": "https://reactome.org/content/detail/R-HSA-264622", "summary": "Cho is acetylated to AcCho by CHAT is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, Cho to CoA-SH, AcCho in cytosol and cross-references RHEA:18822.", "equation": "Ac-CoA + Cho → CoA-SH + AcCho", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-264618", "reactome_id": "R-ALL-264618", "label": "Cho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-264643", "reactome_id": "R-ALL-264643", "label": "AcCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15355", "formula": "C7H16NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "choline O-acetyltransferase activity of CHAT [cytosol]", "activity_id": "264613", "ec_numbers": [ "2.3.1.6" ], "accessions": [ "P28329" ] } ], "citations": [ { "id": "PMID:1339386", "title": "Human choline acetyltransferase (CHAT): partial gene sequence and potential control regions", "journal": "Genomics", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1339386/" } ], "x": 2381.3333333333335, "y": 1636.9109589041095, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-265295", "label": "Prostaglandin E synthase isomerizes PGH2 to PGE2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12894" ], "source_url": "https://reactome.org/content/detail/R-HSA-265295", "summary": "Prostaglandin E synthase isomerizes PGH2 to PGE2 is a Reactome release 97 curated reaction event. The source record connects PGH2 to PGE2 in cytosol and cross-references RHEA:12894.", "equation": "PGH2 → PGE2", "inputs": [ { "node_id": "R-ALL-265283", "reactome_id": "R-ALL-265283", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-265287", "reactome_id": "R-ALL-265287", "label": "PGE2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:606564", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "prostaglandin-E synthase activity of PTGES2(88-377), PTGES3 [cytosol]", "activity_id": "265290", "ec_numbers": [ "5.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:10922363", "title": "Molecular identification of cytosolic prostaglandin E2 synthase that is functionally coupled with cyclooxygenase-1 in immediate prostaglandin E2 biosynthesis", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10922363/" }, { "id": "PMID:12835322", "title": "Cellular prostaglandin E2 production by membrane-bound prostaglandin E synthase-2 via both cyclooxygenases-1 and -2", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12835322/" } ], "x": 868.6666666666667, "y": 1631.0701754385964, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-265296", "label": "Arachidonate is oxidised to 5S-HpETE by ALOX5", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · nuclear envelope", "compartment_ids": [ "GO:0005829", "GO:0005635" ], "rhea_ids": [ "RHEA:17486" ], "source_url": "https://reactome.org/content/detail/R-HSA-265296", "summary": "Arachidonate is oxidised to 5S-HpETE by ALOX5 is a Reactome release 97 curated reaction event. The source record connects AA, O2 to 5S-HpETE in cytosol · nuclear envelope and cross-references RHEA:17486.", "equation": "AA + O2 → 5S-HpETE", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-266010", "reactome_id": "R-ALL-266010", "label": "5S-HpETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57450", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of ALOX5:ALOX5AP:LTC4S [nuclear envelope]", "activity_id": "265275", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:3118366", "title": "Reversible, calcium-dependent membrane association of human leukocyte 5-lipoxygenase", "journal": "Proc Natl Acad Sci U S A", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3118366/" }, { "id": "PMID:3164719", "title": "Characterization of cloned human leukocyte 5-lipoxygenase expressed in mammalian cells", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3164719/" }, { "id": "PMID:3006030", "title": "Single protein from human leukocytes possesses 5-lipoxygenase and leukotriene A4 synthase activities", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3006030/" } ], "x": 868.6666666666667, "y": 1603.9122807017543, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-266012", "label": "LTD4 is converted to LTE4 by DPEP1/2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:48617" ], "source_url": "https://reactome.org/content/detail/R-HSA-266012", "summary": "LTD4 is converted to LTE4 by DPEP1/2 is a Reactome release 97 curated reaction event. The source record connects LTD4, H2O to LTE4, Gly in plasma membrane · extracellular region and cross-references RHEA:48617.", "equation": "LTD4 + H2O → LTE4 + Gly", "inputs": [ { "node_id": "R-ALL-266074", "reactome_id": "R-ALL-266074", "label": "LTD4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63166", "formula": "C25H39N2O6S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-266033", "reactome_id": "R-ALL-266033", "label": "LTE4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57462", "formula": "C23H36NO5S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-266029", "reactome_id": "R-ALL-266029", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dipeptidase activity of DPEP1,2 dimers [plasma membrane]", "activity_id": "266039", "ec_numbers": [ "3.4.13.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:3563417", "title": "Release and functional characterization of the leukotriene D4-metabolizing enzyme (dipeptidase) from human polymorphonuclear leucocytes", "journal": "Scand J Immunol", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3563417/" }, { "id": "PMID:6293969", "title": "Conversion of leukotriene D4 to leukotriene E4 by a dipeptidase released from the specific granule of human polymorphonuclear leucocytes", "journal": "Immunology", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6293969/" }, { "id": "PMID:2768222", "title": "Purification and characterization of human microsomal dipeptidase", "journal": "J Biochem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2768222/" } ], "x": 311.33333333333337, "y": 1576.7543859649122, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-266046", "label": "GGT1, 5 dimers hydrolyse LTC4 to LTD4", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:31564" ], "source_url": "https://reactome.org/content/detail/R-HSA-266046", "summary": "GGT1, 5 dimers hydrolyse LTC4 to LTD4 is a Reactome release 97 curated reaction event. The source record connects LTC4, H2O to LTD4, L-Glu in plasma membrane · extracellular region and cross-references RHEA:31564.", "equation": "LTC4 + H2O → LTD4 + L-Glu", "inputs": [ { "node_id": "R-ALL-266013", "reactome_id": "R-ALL-266013", "label": "LTC4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57973", "formula": "C30H45N3O9S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-266074", "reactome_id": "R-ALL-266074", "label": "LTD4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63166", "formula": "C25H39N2O6S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-210382", "reactome_id": "R-ALL-210382", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione gamma-glutamate hydrolase activity of GGT1, 5 dimers [plasma membrane]", "activity_id": "266030", "ec_numbers": [ "3.4.19.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:6122208", "title": "Interconversion of leukotrienes catalyzed by purified gamma-glutamyl transpeptidase: concomitant formation of leukotriene D4 and gamma-glutamyl amino acids", "journal": "Proc Natl Acad Sci U S A", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6122208/" }, { "id": "PMID:21447318", "title": "Gamma-glutamyl compounds: substrate specificity of gamma-glutamyl transpeptidase enzymes", "journal": "Anal Biochem", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21447318/" } ], "x": 590, "y": 1576.7543859649122, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-266050", "label": "LTA4 is converted to LTC4 by LTC4S", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · nuclear envelope", "compartment_ids": [ "GO:0005829", "GO:0005635" ], "rhea_ids": [ "RHEA:17619" ], "source_url": "https://reactome.org/content/detail/R-HSA-266050", "summary": "LTA4 is converted to LTC4 by LTC4S is a Reactome release 97 curated reaction event. The source record connects LTA4, GSH to LTC4 in cytosol · nuclear envelope and cross-references RHEA:17619.", "equation": "LTA4 + GSH → LTC4", "inputs": [ { "node_id": "R-ALL-265281", "reactome_id": "R-ALL-265281", "label": "LTA4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-266066", "reactome_id": "R-ALL-266066", "label": "LTC4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57973", "formula": "C30H45N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "leukotriene-C4 synthase activity of ALOX5:ALOX5AP:LTC4S [nuclear envelope]", "activity_id": "266055", "ec_numbers": [ "4.4.1.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:8052639", "title": "Expression cloning of a cDNA for human leukotriene C4 synthase, an integral membrane protein conjugating reduced glutathione to leukotriene A4", "journal": "Proc Natl Acad Sci U S A", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8052639/" }, { "id": "PMID:7937884", "title": "Molecular cloning and expression of human leukotriene-C4 synthase", "journal": "Proc Natl Acad Sci U S A", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7937884/" } ], "x": 311.33333333333337, "y": 1606.9298245614034, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-266051", "label": "5S-HpETE is dehydrated to LTA4 by ALOX5", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism", "proresolving-mediators" ], "compartment": "cytosol · nuclear envelope", "compartment_ids": [ "GO:0005829", "GO:0005635" ], "rhea_ids": [ "RHEA:17962" ], "source_url": "https://reactome.org/content/detail/R-HSA-266051", "summary": "5S-HpETE is dehydrated to LTA4 by ALOX5 is a Reactome release 97 curated reaction event. The source record connects 5S-HpETE to LTA4, H2O in cytosol · nuclear envelope and cross-references RHEA:17962.", "equation": "5S-HpETE → LTA4 + H2O", "inputs": [ { "node_id": "R-ALL-266010", "reactome_id": "R-ALL-266010", "label": "5S-HpETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57450", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-265281", "reactome_id": "R-ALL-265281", "label": "LTA4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity of ALOX5:ALOX5AP:LTC4S [nuclear envelope]", "activity_id": "265275", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:3164719", "title": "Characterization of cloned human leukocyte 5-lipoxygenase expressed in mammalian cells", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3164719/" }, { "id": "PMID:3118366", "title": "Reversible, calcium-dependent membrane association of human leukocyte 5-lipoxygenase", "journal": "Proc Natl Acad Sci U S A", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3118366/" }, { "id": "PMID:3006030", "title": "Single protein from human leukocytes possesses 5-lipoxygenase and leukotriene A4 synthase activities", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3006030/" } ], "x": 590, "y": 1606.9298245614034, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-266070", "label": "LTC4 is exported from the cytosol by ABCC1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "plasma membrane · cytosol · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005829", "GO:0005576" ], "rhea_ids": [ "RHEA:38963", "RHEA:38964" ], "source_url": "https://reactome.org/content/detail/R-HSA-266070", "summary": "LTC4 is exported from the cytosol by ABCC1 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects LTC4, ATP, H2O to H+, Pi, LTC4 in plasma membrane · cytosol · extracellular region and cross-references RHEA:38963, RHEA:38964.", "equation": "LTC4 + ATP + H2O → H+ + Pi + LTC4 + ADP", "inputs": [ { "node_id": "R-ALL-266066", "reactome_id": "R-ALL-266066", "label": "LTC4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57973", "formula": "C30H45N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-266013", "reactome_id": "R-ALL-266013", "label": "LTC4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57973", "formula": "C30H45N3O9S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ABC-type transporter activity of ABCC1 [plasma membrane]", "activity_id": "266058", "ec_numbers": null, "accessions": [ "P33527" ] } ], "citations": [ { "id": "PMID:2753893", "title": "The identification of a distinct export step following the biosynthesis of leukotriene C4 by human eosinophils", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2753893/" }, { "id": "PMID:10064732", "title": "Characterization of a leukotriene C4 export mechanism in human platelets: possible involvement of multidrug resistance-associated protein 1", "journal": "J Lipid Res", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10064732/" } ], "x": 311.33333333333337, "y": 1534.5087719298247, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-266072", "label": "LTA4 is hydolysed to LTB4 by LTA4H", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22325" ], "source_url": "https://reactome.org/content/detail/R-HSA-266072", "summary": "LTA4 is hydolysed to LTB4 by LTA4H is a Reactome release 97 curated reaction event. The source record connects LTA4, H2O to LTB4 in cytosol and cross-references RHEA:22325.", "equation": "LTA4 + H2O → LTB4", "inputs": [ { "node_id": "R-ALL-265281", "reactome_id": "R-ALL-265281", "label": "LTA4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-266056", "reactome_id": "R-ALL-266056", "label": "LTB4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57461", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "leukotriene-A4 hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "266024", "ec_numbers": [ "3.3.2.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:6490615", "title": "Leukotriene A4 hydrolase in human leukocytes. Purification and properties", "journal": "J Biol Chem", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6490615/" }, { "id": "PMID:2995393", "title": "Enzymatic hydration of leukotriene A4. Purification and characterization of a novel epoxide hydrolase from human erythrocytes", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2995393/" } ], "x": 868.6666666666667, "y": 1606.9298245614034, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2993447", "label": "HLCS biotinylates 6x(PCCA:PCCB)", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2993447", "summary": "HLCS biotinylates 6x(PCCA:PCCB) is a Reactome release 97 curated reaction event. The source record connects 6x(PCCA:PCCB), Btn, ATP to 6x(Btn-PCCA:PCCB), PPi, AMP in cytosol.", "equation": "6x(PCCA:PCCB) + 6 Btn + 6 ATP → 6x(Btn-PCCA:PCCB) + 6 PPi + 6 AMP", "inputs": [ { "node_id": "R-HSA-2993809", "reactome_id": "R-HSA-2993809", "label": "6x(PCCA:PCCB)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-199207", "reactome_id": "R-ALL-199207", "label": "Btn", "role": "input", "stoichiometry": 6, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 6, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-3323122", "reactome_id": "R-HSA-3323122", "label": "6x(Btn-PCCA:PCCB)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 6, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 6, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "biotin--[biotin carboxyl-carrier protein] ligase activity of HLCS [cytosol]", "activity_id": "2993868", "ec_numbers": [ "6.3.4.10" ], "accessions": [ "P50747" ] } ], "citations": [ { "id": "PMID:16134170", "title": "Mutations in the holocarboxylase synthetase gene HLCS", "journal": "Hum. Mutat.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16134170/" }, { "id": "PMID:22123817", "title": "Selectivity in post-translational biotin addition to five human carboxylases", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22123817/" }, { "id": "PMID:9630604", "title": "Identification of holocarboxylase synthetase (HCS) proteins in human placenta", "journal": "Biochim. Biophys. Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9630604/" }, { "id": "PMID:20153287", "title": "Holocarboxylase synthetase: correlation of protein localisation with biological function", "journal": "Arch. Biochem. Biophys.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20153287/" } ], "x": 5391.333333333333, "y": 3064.0394736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2993799", "label": "HLCS biotinylates 6xMCCC1:6xMCCC2", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2993799", "summary": "HLCS biotinylates 6xMCCC1:6xMCCC2 is a Reactome release 97 curated reaction event. The source record connects 6xMCCC1:6xMCCC2, ATP, Btn to 6x(Btn-MCCC1:MCCC2), PPi, AMP in cytosol.", "equation": "6xMCCC1:6xMCCC2 + 6 ATP + 6 Btn → 6x(Btn-MCCC1:MCCC2) + 6 PPi + 6 AMP", "inputs": [ { "node_id": "R-HSA-3323185", "reactome_id": "R-HSA-3323185", "label": "6xMCCC1:6xMCCC2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 6, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-199207", "reactome_id": "R-ALL-199207", "label": "Btn", "role": "input", "stoichiometry": 6, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3323135", "reactome_id": "R-HSA-3323135", "label": "6x(Btn-MCCC1:MCCC2)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 6, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 6, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "biotin--[biotin carboxyl-carrier protein] ligase activity of HLCS [cytosol]", "activity_id": "2993868", "ec_numbers": [ "6.3.4.10" ], "accessions": [ "P50747" ] } ], "citations": [ { "id": "PMID:16134170", "title": "Mutations in the holocarboxylase synthetase gene HLCS", "journal": "Hum. Mutat.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16134170/" }, { "id": "PMID:20153287", "title": "Holocarboxylase synthetase: correlation of protein localisation with biological function", "journal": "Arch. Biochem. Biophys.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20153287/" }, { "id": "PMID:9630604", "title": "Identification of holocarboxylase synthetase (HCS) proteins in human placenta", "journal": "Biochim. Biophys. Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9630604/" }, { "id": "PMID:22123817", "title": "Selectivity in post-translational biotin addition to five human carboxylases", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22123817/" } ], "x": 5670, "y": 3064.0394736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2993802", "label": "HLCS biotinylates PC:Mn2+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2993802", "summary": "HLCS biotinylates PC:Mn2+ is a Reactome release 97 curated reaction event. The source record connects 4x(PC:Mn2+), ATP, Btn to 4x(Btn-PC:Mn2+), PPi, AMP in cytosol.", "equation": "4x(PC:Mn2+) + 4 ATP + 4 Btn → 4x(Btn-PC:Mn2+) + 4 PPi + 4 AMP", "inputs": [ { "node_id": "R-HSA-2993798", "reactome_id": "R-HSA-2993798", "label": "4x(PC:Mn2+)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-199207", "reactome_id": "R-ALL-199207", "label": "Btn", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3323188", "reactome_id": "R-HSA-3323188", "label": "4x(Btn-PC:Mn2+)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "biotin--[biotin carboxyl-carrier protein] ligase activity of HLCS [cytosol]", "activity_id": "2993868", "ec_numbers": [ "6.3.4.10" ], "accessions": [ "P50747" ] } ], "citations": [ { "id": "PMID:18297087", "title": "Crystal structures of human and Staphylococcus aureus pyruvate carboxylase and molecular insights into the carboxyltransfer reaction", "journal": "Nat. Struct. Mol. Biol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18297087/" }, { "id": "PMID:16134170", "title": "Mutations in the holocarboxylase synthetase gene HLCS", "journal": "Hum. Mutat.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16134170/" }, { "id": "PMID:20153287", "title": "Holocarboxylase synthetase: correlation of protein localisation with biological function", "journal": "Arch. Biochem. Biophys.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20153287/" }, { "id": "PMID:9630604", "title": "Identification of holocarboxylase synthetase (HCS) proteins in human placenta", "journal": "Biochim. Biophys. Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9630604/" }, { "id": "PMID:22123817", "title": "Selectivity in post-translational biotin addition to five human carboxylases", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22123817/" } ], "x": 5948.666666666667, "y": 3064.0394736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2993814", "label": "HLCS biotinylates ACACA:Mn2+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2993814", "summary": "HLCS biotinylates ACACA:Mn2+ is a Reactome release 97 curated reaction event. The source record connects ACACA:2Mn2+, Btn, ATP to Btn-ACACA:2Mn2+, AMP, PPi in cytosol.", "equation": "ACACA:2Mn2+ + Btn + ATP → Btn-ACACA:2Mn2+ + AMP + PPi", "inputs": [ { "node_id": "R-HSA-2993826", "reactome_id": "R-HSA-2993826", "label": "ACACA:2Mn2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-199207", "reactome_id": "R-ALL-199207", "label": "Btn", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-2993815", "reactome_id": "R-HSA-2993815", "label": "Btn-ACACA:2Mn2+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "biotin--[biotin carboxyl-carrier protein] ligase activity of HLCS [cytosol]", "activity_id": "2993868", "ec_numbers": [ "6.3.4.10" ], "accessions": [ "P50747" ] } ], "citations": [ { "id": "PMID:16134170", "title": "Mutations in the holocarboxylase synthetase gene HLCS", "journal": "Hum. Mutat.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16134170/" }, { "id": "PMID:20153287", "title": "Holocarboxylase synthetase: correlation of protein localisation with biological function", "journal": "Arch. Biochem. Biophys.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20153287/" }, { "id": "PMID:9630604", "title": "Identification of holocarboxylase synthetase (HCS) proteins in human placenta", "journal": "Biochim. Biophys. Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9630604/" }, { "id": "PMID:22123817", "title": "Selectivity in post-translational biotin addition to five human carboxylases", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22123817/" }, { "id": "PMID:10677481", "title": "The subcellular localization of acetyl-CoA carboxylase 2", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10677481/" }, { "id": "PMID:7732023", "title": "Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7732023/" } ], "x": 5391.333333333333, "y": 3074.2763157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2995330", "label": "COX10 transforms heme to heme O", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-2995330", "summary": "COX10 transforms heme to heme O is a Reactome release 97 curated reaction event. The source record connects FPP, H2O, heme b to PPi, heme O in mitochondrial matrix · mitochondrial inner membrane.", "equation": "FPP + H2O + heme b → PPi + heme O", "inputs": [ { "node_id": "R-ALL-2162257", "reactome_id": "R-ALL-2162257", "label": "FPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:175763", "formula": "C15H25O7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9853511", "reactome_id": "R-ALL-9853511", "label": "heme b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:26355", "formula": "C34H32FeN4O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9865393", "reactome_id": "R-ALL-9865393", "label": "heme O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60530", "formula": "C49H56FeN4O5", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "protoheme IX farnesyltransferase activity of COX10(?-443) [mitochondrial inner membrane]", "activity_id": "2995336", "ec_numbers": [ "2.5.1.141" ], "accessions": [ "Q12887" ] } ], "citations": [ { "id": "PMID:8078902", "title": "Isolation of a human cDNA for heme A:farnesyltransferase by functional complementation of a yeast cox10 mutant", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8078902/" }, { "id": "PMID:9177788", "title": "Genomic structure and expression of the human heme A:farnesyltransferase (COX10) gene", "journal": "Genomics", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9177788/" }, { "id": "PMID:34428995", "title": "Biosynthesis and trafficking of heme o and heme a : new structural insights and their implications for reaction mechanisms and prenylated heme transfer", "journal": "Crit Rev Biochem Mol Biol", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34428995/" } ], "x": 7078.666666666667, "y": 2955.8214285714284, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-2995334", "label": "COX15 transforms heme O to heme A", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:63388" ], "source_url": "https://reactome.org/content/detail/R-HSA-2995334", "summary": "COX15 transforms heme O to heme A is a Reactome release 97 curated reaction event. The source record connects hydrogen acceptor, H2O, heme O to hydrogen donor, heme A in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:63388.", "equation": "2 hydrogen acceptor + H2O + heme O → 2 hydrogen donor + heme A", "inputs": [ { "node_id": "R-ALL-9757044", "reactome_id": "R-ALL-9757044", "label": "hydrogen acceptor", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:13193", "formula": "A", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9865393", "reactome_id": "R-ALL-9865393", "label": "heme O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60530", "formula": "C49H56FeN4O5", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9757039", "reactome_id": "R-ALL-9757039", "label": "hydrogen donor", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:17499", "formula": "H2A", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9864492", "reactome_id": "R-ALL-9864492", "label": "heme A", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61715", "formula": "C49H54FeN4O6", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "heme A synthase activity of COX15 [mitochondrial inner membrane]", "activity_id": "2995322", "ec_numbers": [ "1.17.99.9" ], "accessions": [ "Q7KZN9" ] } ], "citations": [ { "id": "PMID:12474143", "title": "Mutations in COX15 produce a defect in the mitochondrial heme biosynthetic pathway, causing early-onset fatal hypertrophic cardiomyopathy", "journal": "Am. J. Hum. Genet.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12474143/" }, { "id": "PMID:15863660", "title": "Novel mutations in COX15 in a long surviving Leigh syndrome patient with cytochrome c oxidase deficiency", "journal": "J. Med. Genet.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15863660/" }, { "id": "PMID:15235026", "title": "Functional and genetic studies demonstrate that mutation in the COX15 gene can cause Leigh syndrome", "journal": "J. Med. Genet.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15235026/" }, { "id": "PMID:9878253", "title": "Identification and characterization of human cDNAs specific to BCS1, PET112, SCO1, COX15, and COX11, five genes involved in the formation and function of the mitochondrial respiratory chain", "journal": "Genomics", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9878253/" }, { "id": "PMID:34428995", "title": "Biosynthesis and trafficking of heme o and heme a : new structural insights and their implications for reaction mechanisms and prenylated heme transfer", "journal": "Crit Rev Biochem Mol Biol", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34428995/" } ], "x": 6800, "y": 2844.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3000074", "label": "TCN2 binds RCbl in the circulation", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3000074", "summary": "TCN2 binds RCbl in the circulation is a Reactome release 97 curated reaction event. The source record connects TCN2, RCbl to TCN2:RCbl in extracellular region.", "equation": "TCN2 + RCbl → TCN2:RCbl", "inputs": [ { "node_id": "R-HSA-2980925", "reactome_id": "R-HSA-2980925", "label": "TCN2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9759382", "reactome_id": "R-ALL-9759382", "label": "RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9759429", "reactome_id": "R-HSA-9759429", "label": "TCN2:RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor ligand activity of TCN2 [extracellular region]", "activity_id": "9763688", "ec_numbers": null, "accessions": [ "P20062" ] } ], "citations": [ { "id": "PMID:27411955", "title": "Structural basis of transcobalamin recognition by human CD320 receptor", "journal": "Nat Commun", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27411955/" }, { "id": "PMID:2777761", "title": "Structure of the cDNA encoding transcobalamin I, a neutrophil granule protein", "journal": "J. Biol. Chem.", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2777761/" }, { "id": "PMID:3782074", "title": "Purification and molecular characterization of human transcobalamin II", "journal": "J. Biol. Chem.", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3782074/" }, { "id": "PMID:16537422", "title": "Structural basis for mammalian vitamin B12 transport by transcobalamin", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16537422/" } ], "x": 5670, "y": 3115.2236842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3000103", "label": "CUBN:AMN binds CBLIF:RCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3000103", "summary": "CUBN:AMN binds CBLIF:RCbl is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CBLIF:RCbl, CUBN:AMN to CUBN:AMN:CBLIF:RCbl in plasma membrane · extracellular region.", "equation": "CBLIF:RCbl + CUBN:AMN → CUBN:AMN:CBLIF:RCbl", "inputs": [ { "node_id": "R-HSA-3000147", "reactome_id": "R-HSA-3000147", "label": "CBLIF:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-264830", "reactome_id": "R-HSA-264830", "label": "CUBN:AMN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3000141", "reactome_id": "R-HSA-3000141", "label": "CUBN:AMN:CBLIF:RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of CUBN:AMN [plasma membrane]", "activity_id": "9758896", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:17954916", "title": "Crystal structure of human intrinsic factor: cobalamin complex at 2.6-A resolution", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17954916/" }, { "id": "PMID:14576052", "title": "The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless", "journal": "Blood", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14576052/" }, { "id": "PMID:20237569", "title": "Structural basis for receptor recognition of vitamin-B(12)-intrinsic factor complexes", "journal": "Nature", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20237569/" }, { "id": "PMID:10080186", "title": "Mutations in CUBN, encoding the intrinsic factor-vitamin B12 receptor, cubilin, cause hereditary megaloblastic anaemia 1", "journal": "Nat. Genet.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10080186/" }, { "id": "PMID:10887099", "title": "Cubilin P1297L mutation associated with hereditary megaloblastic anemia 1 causes impaired recognition of intrinsic factor-vitamin B(12) by cubilin", "journal": "Blood", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10887099/" }, { "id": "PMID:30523278", "title": "Structural assembly of the megadalton-sized receptor for intestinal vitamin B 12 uptake and kidney protein reabsorption", "journal": "Nat Commun", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30523278/" } ], "x": 5391.333333333333, "y": 3135.6973684210525, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-3000112", "label": "CD320-mediated TCN2:RCbl uptake and delivery to lysosome", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane · lysosomal lumen · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0043202", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3000112", "summary": "CD320-mediated TCN2:RCbl uptake and delivery to lysosome is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects TCN2:RCbl:CD320 to CD320, TCN2:RCbl in plasma membrane · lysosomal lumen · extracellular region.", "equation": "TCN2:RCbl:CD320 → CD320 + TCN2:RCbl", "inputs": [ { "node_id": "R-HSA-3000089", "reactome_id": "R-HSA-3000089", "label": "TCN2:RCbl:CD320", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-2980909", "reactome_id": "R-HSA-2980909", "label": "CD320", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-3000242", "reactome_id": "R-HSA-3000242", "label": "TCN2:RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of TCN2:RCbl:CD320 [plasma membrane]", "activity_id": "9759384", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:27411955", "title": "Structural basis of transcobalamin recognition by human CD320 receptor", "journal": "Nat Commun", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27411955/" }, { "id": "PMID:759210", "title": "Protein mediated vitamin uptake. Adsorptive endocytosis of the transcobalamin II-cobalamin complex by cultured human fibroblasts", "journal": "Exp. Cell Res.", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/759210/" } ], "x": 5391.333333333333, "y": 3176.6447368421054, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-3000120", "label": "CBLIF binds RCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3000120", "summary": "CBLIF binds RCbl is a Reactome release 97 curated reaction event. The source record connects CBLIF, RCbl to CBLIF:RCbl in extracellular region.", "equation": "CBLIF + RCbl → CBLIF:RCbl", "inputs": [ { "node_id": "R-HSA-2980918", "reactome_id": "R-HSA-2980918", "label": "CBLIF", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9759382", "reactome_id": "R-ALL-9759382", "label": "RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3000147", "reactome_id": "R-HSA-3000147", "label": "CBLIF:RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor ligand activity of CBLIF [extracellular region]", "activity_id": "9763690", "ec_numbers": null, "accessions": [ "P27352" ] } ], "citations": [ { "id": "PMID:17954916", "title": "Crystal structure of human intrinsic factor: cobalamin complex at 2.6-A resolution", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17954916/" } ], "x": 5948.666666666667, "y": 3115.2236842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3000122", "label": "CD320 binds extracellular TCN2:RCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region · endosome membrane · endosome", "compartment_ids": [ "GO:0005576", "GO:0010008", "GO:0005768" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3000122", "summary": "CD320 binds extracellular TCN2:RCbl is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CD320, TCN2:RCbl to TCN2:RCbl:CD320 in extracellular region · endosome membrane · endosome.", "equation": "CD320 + TCN2:RCbl → TCN2:RCbl:CD320", "inputs": [ { "node_id": "R-HSA-2980909", "reactome_id": "R-HSA-2980909", "label": "CD320", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9759429", "reactome_id": "R-HSA-9759429", "label": "TCN2:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3000089", "reactome_id": "R-HSA-3000089", "label": "TCN2:RCbl:CD320", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of CD320 [plasma membrane]", "activity_id": "3000088", "ec_numbers": null, "accessions": [ "Q9NPF0" ] } ], "citations": [ { "id": "PMID:15652495", "title": "The binding properties of the human receptor for the cellular uptake of vitamin B12", "journal": "Biochem. Biophys. Res. Commun.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15652495/" }, { "id": "PMID:18779389", "title": "The protein and the gene encoding the receptor for the cellular uptake of transcobalamin-bound cobalamin", "journal": "Blood", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18779389/" }, { "id": "PMID:20524213", "title": "Positive newborn screen for methylmalonic aciduria identifies the first mutation in TCblR/CD320, the gene for cellular uptake of transcobalamin-bound vitamin B(12)", "journal": "Hum. Mutat.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20524213/" } ], "x": 5670, "y": 3135.6973684210525, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-3000137", "label": "CUBN:AMN-mediated CBLIF:RCbl uptake and delivery to lysosome", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region · plasma membrane · lysosomal lumen", "compartment_ids": [ "GO:0005576", "GO:0005886", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3000137", "summary": "CUBN:AMN-mediated CBLIF:RCbl uptake and delivery to lysosome is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CUBN:AMN:CBLIF:RCbl to CBLIF:RCbl, CUBN:AMN in extracellular region · plasma membrane · lysosomal lumen.", "equation": "CUBN:AMN:CBLIF:RCbl → CBLIF:RCbl + CUBN:AMN", "inputs": [ { "node_id": "R-HSA-3000141", "reactome_id": "R-HSA-3000141", "label": "CUBN:AMN:CBLIF:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3000280", "reactome_id": "R-HSA-3000280", "label": "CBLIF:RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-264830", "reactome_id": "R-HSA-264830", "label": "CUBN:AMN", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of CUBN:AMN:CBLIF:RCbl [plasma membrane]", "activity_id": "9759386", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:14576052", "title": "The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless", "journal": "Blood", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14576052/" } ], "x": 5670, "y": 3176.6447368421054, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-3000243", "label": "Unknown lysosomal protease degrades CBLIF:RCbl to release Cbl", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3000243", "summary": "Unknown lysosomal protease degrades CBLIF:RCbl to release Cbl is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CBLIF:RCbl to RCbl in lysosomal lumen.", "equation": "CBLIF:RCbl → RCbl", "inputs": [ { "node_id": "R-HSA-3000280", "reactome_id": "R-HSA-3000280", "label": "CBLIF:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9759390", "reactome_id": "R-ALL-9759390", "label": "RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "peptidase activity of unknown protease [lysosomal lumen]", "activity_id": "8960084", "ec_numbers": [ "3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:14576052", "title": "The functional cobalamin (vitamin B12)-intrinsic factor receptor is a novel complex of cubilin and amnionless", "journal": "Blood", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14576052/" } ], "x": 5391.333333333333, "y": 3156.171052631579, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-3000263", "label": "TCN2:RCbl is degraded to release RCbl", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3000263", "summary": "TCN2:RCbl is degraded to release RCbl is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects TCN2:RCbl to RCbl in lysosomal lumen.", "equation": "TCN2:RCbl → RCbl", "inputs": [ { "node_id": "R-HSA-3000242", "reactome_id": "R-HSA-3000242", "label": "TCN2:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9759390", "reactome_id": "R-ALL-9759390", "label": "RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "peptidase activity of unknown protease [lysosomal lumen]", "activity_id": "8960084", "ec_numbers": [ "3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:759210", "title": "Protein mediated vitamin uptake. Adsorptive endocytosis of the transcobalamin II-cobalamin complex by cultured human fibroblasts", "journal": "Exp. Cell Res.", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/759210/" } ], "x": 5670, "y": 3156.171052631579, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-3065958", "label": "An unknown protease degrades ACACA", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3065958", "summary": "An unknown protease degrades ACACA is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Btn-ACACA:2Mn2+ to BCTN in cytosol.", "equation": "Btn-ACACA:2Mn2+ → BCTN", "inputs": [ { "node_id": "R-HSA-2993815", "reactome_id": "R-HSA-2993815", "label": "Btn-ACACA:2Mn2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-3065957", "reactome_id": "R-ALL-3065957", "label": "BCTN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195545", "formula": "C16H28N4O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "peptidase activity of unknown peptidase [cytosol]", "activity_id": "3076897", "ec_numbers": [ "3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:10064314", "title": "Human biotinidase isn't just for recycling biotin", "journal": "J. Nutr.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10064314/" }, { "id": "PMID:8930409", "title": "Biotinidase and its roles in biotin metabolism", "journal": "Clin. Chim. Acta", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8930409/" }, { "id": "PMID:2868710", "title": "Distribution and degradation of biotin-containing carboxylases in human cell lines", "journal": "Biochem. J.", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2868710/" } ], "x": 5948.666666666667, "y": 3156.171052631579, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-3065959", "label": "An unknown protease degrades hCBXs", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3065959", "summary": "An unknown protease degrades hCBXs is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects hCBXs to BCTN in mitochondrial matrix.", "equation": "hCBXs → BCTN", "inputs": [ { "node_id": "R-HSA-3065950", "reactome_id": "R-HSA-3065950", "label": "hCBXs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-3065953", "reactome_id": "R-ALL-3065953", "label": "BCTN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195545", "formula": "C16H28N4O4S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "peptidase activity of unknown peptidase [mitochondrial matrix]", "activity_id": "3076885", "ec_numbers": [ "3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:2868710", "title": "Distribution and degradation of biotin-containing carboxylases in human cell lines", "journal": "Biochem. J.", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2868710/" }, { "id": "PMID:10064314", "title": "Human biotinidase isn't just for recycling biotin", "journal": "J. Nutr.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10064314/" }, { "id": "PMID:8930409", "title": "Biotinidase and its roles in biotin metabolism", "journal": "Clin. Chim. Acta", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8930409/" } ], "x": 5391.333333333333, "y": 3166.407894736842, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-3076881", "label": "BCTN translocates to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3076881", "summary": "BCTN translocates to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects BCTN to BCTN in cytosol · extracellular region.", "equation": "BCTN → BCTN", "inputs": [ { "node_id": "R-ALL-3065957", "reactome_id": "R-ALL-3065957", "label": "BCTN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195545", "formula": "C16H28N4O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-3076887", "reactome_id": "R-ALL-3076887", "label": "BCTN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195545", "formula": "C16H28N4O4S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:2868710", "title": "Distribution and degradation of biotin-containing carboxylases in human cell lines", "journal": "Biochem. J.", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2868710/" } ], "x": 5670, "y": 3186.8815789473683, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-3076905", "label": "Extracellular BTD hydrolyses BCTN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [ "RHEA:77172" ], "source_url": "https://reactome.org/content/detail/R-HSA-3076905", "summary": "Extracellular BTD hydrolyses BCTN is a Reactome release 97 curated reaction event. The source record connects BCTN to Btn, L-Lys in extracellular region and cross-references RHEA:77172.", "equation": "BCTN → Btn + L-Lys", "inputs": [ { "node_id": "R-ALL-3076887", "reactome_id": "R-ALL-3076887", "label": "BCTN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195545", "formula": "C16H28N4O4S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-199238", "reactome_id": "R-ALL-199238", "label": "Btn", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351991", "reactome_id": "R-ALL-351991", "label": "L-Lys", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32551", "formula": "C6H15N2O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "biotinidase activity of BTD [extracellular region]", "activity_id": "3076906", "ec_numbers": [ "3.5.1.12" ], "accessions": [ "P43251" ] } ], "citations": [ { "id": "PMID:7509806", "title": "Human serum biotinidase. cDNA cloning, sequence, and characterization", "journal": "J. Biol. Chem.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7509806/" }, { "id": "PMID:6883721", "title": "Biotinidase deficiency: the enzymatic defect in late-onset multiple carboxylase deficiency", "journal": "Clin. Chim. Acta", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6883721/" }, { "id": "PMID:16150625", "title": "Evolutionary conservation of biotinidase: implications for the enzyme's structure and subcellular localization", "journal": "Mol. Genet. Metab.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16150625/" } ], "x": 5948.666666666667, "y": 3002.6184210526317, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3095889", "label": "MMACHC dealkylates RCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:40720" ], "source_url": "https://reactome.org/content/detail/R-HSA-3095889", "summary": "MMACHC dealkylates RCbl is a Reactome release 97 curated reaction event. The source record connects MMACHC, RCbl, GSH to R-GSH, MMACHC:cob(I)alamin, H+ in cytosol and cross-references RHEA:40720.", "equation": "MMACHC + RCbl + GSH → R-GSH + MMACHC:cob(I)alamin + H+", "inputs": [ { "node_id": "R-HSA-2980949", "reactome_id": "R-HSA-2980949", "label": "MMACHC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9759394", "reactome_id": "R-ALL-9759394", "label": "RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9759438", "reactome_id": "R-ALL-9759438", "label": "R-GSH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90779", "formula": "C10H15N3O6SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9759561", "reactome_id": "R-HSA-9759561", "label": "MMACHC:cob(I)alamin", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "transferase activity, transferring alkyl or aryl (other than methyl) groups of MMACHC [cytosol]", "activity_id": "3245899", "ec_numbers": [ "2.5" ], "accessions": [ "Q9Y4U1" ] } ], "citations": [ { "id": "PMID:19447654", "title": "Processing of alkylcobalamins in mammalian cells: A role for the MMACHC (cblC) gene product", "journal": "Mol. Genet. Metab.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19447654/" }, { "id": "PMID:19801555", "title": "A human vitamin B12 trafficking protein uses glutathione transferase activity for processing alkylcobalamins", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19801555/" }, { "id": "PMID:21697092", "title": "Structural basis of multifunctionality in a vitamin B12-processing enzyme", "journal": "J Biol Chem", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21697092/" }, { "id": "PMID:16311595", "title": "Identification of the gene responsible for methylmalonic aciduria and homocystinuria, cblC type", "journal": "Nat. Genet.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16311595/" } ], "x": 5948.666666666667, "y": 2971.907894736842, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3095901", "label": "ABCC1 transports cytosolic RCbl to extracellular region", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · lysosomal lumen · lysosomal membrane", "compartment_ids": [ "GO:0005829", "GO:0043202", "GO:0005765" ], "rhea_ids": [ "RHEA:17873" ], "source_url": "https://reactome.org/content/detail/R-HSA-3095901", "summary": "ABCC1 transports cytosolic RCbl to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATP, H2O, RCbl to ADP, Pi, RCbl in cytosol · lysosomal lumen · lysosomal membrane and cross-references RHEA:17873.", "equation": "ATP + H2O + RCbl → ADP + Pi + RCbl", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9759394", "reactome_id": "R-ALL-9759394", "label": "RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9759382", "reactome_id": "R-ALL-9759382", "label": "RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ABC-type vitamin B12 transporter activity of ABCC1 [plasma membrane]", "activity_id": "3095900", "ec_numbers": [ "7.6.2.8" ], "accessions": [ "P33527" ] } ], "citations": [ { "id": "PMID:22547309", "title": "Vitamin B12 transport from food to the body's cells--a sophisticated, multistep pathway", "journal": "Nat Rev Gastroenterol Hepatol", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22547309/" }, { "id": "PMID:21511966", "title": "Investigation of the ABC transporter MRP1 in selected patients with presumed defects in vitamin B12 absorption", "journal": "Blood", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21511966/" } ], "x": 5670, "y": 2890.0131578947367, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-3132753", "label": "Pancreatic proteases degrade TCN1:RCbl", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3132753", "summary": "Pancreatic proteases degrade TCN1:RCbl is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects TCN1:RCbl to RCbl in extracellular region.", "equation": "TCN1:RCbl → RCbl", "inputs": [ { "node_id": "R-HSA-3132765", "reactome_id": "R-HSA-3132765", "label": "TCN1:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9759382", "reactome_id": "R-ALL-9759382", "label": "RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "serine-type peptidase activity of PRSS1,3,CTRB1,2 [extracellular region]", "activity_id": "3132770", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:22900303", "title": "Effect of gastrointestinal proteases on purified human intrinsic factor-vitamin B12 (IF-B12) complex", "journal": "Indian J. Biochem. Biophys.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/22900303/" }, { "id": "PMID:22547309", "title": "Vitamin B12 transport from food to the body's cells--a sophisticated, multistep pathway", "journal": "Nat Rev Gastroenterol Hepatol", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22547309/" } ], "x": 5670, "y": 3166.407894736842, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-3132759", "label": "TCN1 binds RCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3132759", "summary": "TCN1 binds RCbl is a Reactome release 97 curated reaction event. The source record connects TCN1, dietary protein:RCbl to TCN1:RCbl, dietary protein in extracellular region.", "equation": "TCN1 + dietary protein:RCbl → TCN1:RCbl + dietary protein", "inputs": [ { "node_id": "R-HSA-2980920", "reactome_id": "R-HSA-2980920", "label": "TCN1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-3132775", "reactome_id": "R-ALL-3132775", "label": "dietary protein:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3132765", "reactome_id": "R-HSA-3132765", "label": "TCN1:RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-8984753", "reactome_id": "R-ALL-8984753", "label": "dietary protein", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "cargo receptor ligand activity of TCN1 [extracellular region]", "activity_id": "9763691", "ec_numbers": null, "accessions": [ "P20061" ] } ], "citations": [ { "id": "PMID:17990953", "title": "Haptocorrin in humans", "journal": "Clin Chem Lab Med", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17990953/" }, { "id": "PMID:22547309", "title": "Vitamin B12 transport from food to the body's cells--a sophisticated, multistep pathway", "journal": "Nat Rev Gastroenterol Hepatol", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22547309/" }, { "id": "PMID:30777892", "title": "A deep proteome and transcriptome abundance atlas of 29 healthy human tissues", "journal": "Mol Syst Biol", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30777892/" } ], "x": 5948.666666666667, "y": 3135.6973684210525, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3149494", "label": "MMACHC:cob(II)alamin binds MMADHC", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3149494", "summary": "MMACHC:cob(II)alamin binds MMADHC is a Reactome release 97 curated reaction event. The source record connects MMACHC:cob(II)alamin, MMADHC to MMACHC:MMADHC:cob(II)alamin in cytosol.", "equation": "MMACHC:cob(II)alamin + MMADHC → MMACHC:MMADHC:cob(II)alamin", "inputs": [ { "node_id": "R-HSA-3095903", "reactome_id": "R-HSA-3095903", "label": "MMACHC:cob(II)alamin", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-3149529", "reactome_id": "R-HSA-3149529", "label": "MMADHC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3149533", "reactome_id": "R-HSA-3149533", "label": "MMACHC:MMADHC:cob(II)alamin", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of MMADHC [cytosol]", "activity_id": "9908090", "ec_numbers": null, "accessions": [ "Q9H3L0" ] } ], "citations": [ { "id": "PMID:33797888", "title": "Redox-Linked Coordination Chemistry Directs Vitamin B 12 Trafficking", "journal": "Acc Chem Res", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33797888/" }, { "id": "PMID:23270877", "title": "Subcellular location of MMACHC and MMADHC, two human proteins central to intracellular vitamin B(12) metabolism", "journal": "Mol. Genet. Metab.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23270877/" }, { "id": "PMID:21071249", "title": "Interaction between MMACHC and MMADHC, two human proteins participating in intracellular vitamin B?? metabolism", "journal": "Mol. Genet. Metab.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21071249/" }, { "id": "PMID:22832074", "title": "Structural features of recombinant MMADHC isoforms and their interactions with MMACHC, proteins of mammalian vitamin B12 metabolism", "journal": "Mol. Genet. Metab.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22832074/" }, { "id": "PMID:18385497", "title": "Gene identification for the cblD defect of vitamin B12 metabolism", "journal": "N. Engl. J. Med.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18385497/" } ], "x": 5391.333333333333, "y": 3145.934210526316, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3149518", "label": "MTRR reduces cob(II)alamin to meCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23910" ], "source_url": "https://reactome.org/content/detail/R-HSA-3149518", "summary": "MTRR reduces cob(II)alamin to meCbl is a Reactome release 97 curated reaction event. The source record connects MTRR:MTR:cob(II)alamin, NADPH, AdoMet to MTRR:MTR:MeCbl, AdoHcy, NADP+ in cytosol and cross-references RHEA:23910.", "equation": "2 MTRR:MTR:cob(II)alamin + NADPH + 2 AdoMet → 2 MTRR:MTR:MeCbl + 2 AdoHcy + NADP+ + H+", "inputs": [ { "node_id": "R-HSA-3149544", "reactome_id": "R-HSA-3149544", "label": "MTRR:MTR:cob(II)alamin", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3149551", "reactome_id": "R-HSA-3149551", "label": "MTRR:MTR:MeCbl", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "[methionine synthase] reductase (NADPH) activity of MTRR:MTR:cob(II)alamin [cytosol]", "activity_id": "3149528", "ec_numbers": [ "1.16.1.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:12375236", "title": "Maternal genetic effects, exerted by genes involved in homocysteine remethylation, influence the risk of spina bifida", "journal": "Am. J. Hum. Genet.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12375236/" }, { "id": "PMID:10978155", "title": "Interaction of flavodoxin with cobalamin-dependent methionine synthase", "journal": "Biochemistry", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10978155/" }, { "id": "PMID:10444342", "title": "A common variant in methionine synthase reductase combined with low cobalamin (vitamin B12) increases risk for spina bifida", "journal": "Mol. Genet. Metab.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10444342/" }, { "id": "PMID:10484769", "title": "Molecular basis for methionine synthase reductase deficiency in patients belonging to the cblE complementation group of disorders in folate/cobalamin metabolism", "journal": "Hum. Mol. Genet.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10484769/" }, { "id": "PMID:19243433", "title": "Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase-methionine synthase reductase complex", "journal": "FEBS J.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19243433/" }, { "id": "PMID:17892308", "title": "Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17892308/" } ], "x": 5948.666666666667, "y": 2930.9605263157896, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3149519", "label": "MMACHC decyanates CNCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16115" ], "source_url": "https://reactome.org/content/detail/R-HSA-3149519", "summary": "MMACHC decyanates CNCbl is a Reactome release 97 curated reaction event. The source record connects CNCbl, NADPH, H+ to MMACHC:cob(II)alamin, HCN, NADP+ in cytosol and cross-references RHEA:16115.", "equation": "2 CNCbl + NADPH + H+ + 2 MMACHC → 2 MMACHC:cob(II)alamin + 2 HCN + NADP+", "inputs": [ { "node_id": "R-ALL-3149505", "reactome_id": "R-ALL-3149505", "label": "CNCbl", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:17439", "formula": "C63H88CoN14O14P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-2980949", "reactome_id": "R-HSA-2980949", "label": "MMACHC", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3095903", "reactome_id": "R-HSA-3095903", "label": "MMACHC:cob(II)alamin", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-1130611", "reactome_id": "R-ALL-1130611", "label": "HCN", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18407", "formula": "CHN", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "cyanocobalamin reductase (cyanide-eliminating) (NADP+) activity of MMACHC [cytosol]", "activity_id": "3149491", "ec_numbers": [ "1.16.1.6" ], "accessions": [ "Q9Y4U1" ] } ], "citations": [ { "id": "PMID:18779575", "title": "Decyanation of vitamin B12 by a trafficking chaperone", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18779575/" }, { "id": "PMID:21697092", "title": "Structural basis of multifunctionality in a vitamin B12-processing enzyme", "journal": "J Biol Chem", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21697092/" }, { "id": "PMID:20657474", "title": "Vitamin B12: unique metalorganic compounds and the most complex vitamins", "journal": "Molecules", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20657474/" } ], "x": 5391.333333333333, "y": 2941.1973684210525, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3149539", "label": "MTR transfers CH3 group from 5-methyl-THF to cob(I)alamin", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3149539", "summary": "MTR transfers CH3 group from 5-methyl-THF to cob(I)alamin is a Reactome release 97 curated reaction event. The source record connects MTRR:MTR:cob(I)alamin, 5-methyl-THF to MTRR:MTR:MeCbl, THF in cytosol.", "equation": "MTRR:MTR:cob(I)alamin + 5-methyl-THF → MTRR:MTR:MeCbl + THF", "inputs": [ { "node_id": "R-HSA-3149516", "reactome_id": "R-HSA-3149516", "label": "MTRR:MTR:cob(I)alamin", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-200665", "reactome_id": "R-ALL-200665", "label": "5-methyl-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18608", "formula": "C20H23N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3149551", "reactome_id": "R-HSA-3149551", "label": "MTRR:MTR:MeCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-111355", "reactome_id": "R-ALL-111355", "label": "THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methionine synthase activity of MTRR:MTR:cob(I)alamin [cytosol]", "activity_id": "3149542", "ec_numbers": [ "2.1.1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:8968737", "title": "Human methionine synthase: cDNA cloning and identification of mutations in patients of the cblG complementation group of folate/cobalamin disorders", "journal": "Hum Mol Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8968737/" } ], "x": 5948.666666666667, "y": 3104.9868421052633, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3149563", "label": "MMADHC targets transport of cytosolic cob(II)alamin to mitochondria", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3149563", "summary": "MMADHC targets transport of cytosolic cob(II)alamin to mitochondria is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects MMACHC:MMADHC:cob(II)alamin to cob(II)alamin, MMADHC, MMACHC in cytosol · mitochondrial matrix.", "equation": "MMACHC:MMADHC:cob(II)alamin → cob(II)alamin + MMADHC + MMACHC", "inputs": [ { "node_id": "R-HSA-3149533", "reactome_id": "R-HSA-3149533", "label": "MMACHC:MMADHC:cob(II)alamin", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-3149564", "reactome_id": "R-ALL-3149564", "label": "cob(II)alamin", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16304", "formula": "C62H88CoN13O14P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3149529", "reactome_id": "R-HSA-3149529", "label": "MMADHC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2980949", "reactome_id": "R-HSA-2980949", "label": "MMACHC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of MMACHC:MMADHC:cob(II)alamin [cytosol]", "activity_id": "9908939", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:33797888", "title": "Redox-Linked Coordination Chemistry Directs Vitamin B 12 Trafficking", "journal": "Acc Chem Res", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33797888/" }, { "id": "PMID:18385497", "title": "Gene identification for the cblD defect of vitamin B12 metabolism", "journal": "N. Engl. J. Med.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18385497/" }, { "id": "PMID:22156578", "title": "Molecular mechanisms leading to three different phenotypes in the cblD defect of intracellular cobalamin metabolism", "journal": "Hum. Mol. Genet.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22156578/" } ], "x": 5391.333333333333, "y": 3125.4605263157896, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-3159253", "label": "MMAB adenosylates cob(I)alamin", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3159253", "summary": "MMAB adenosylates cob(I)alamin is a Reactome release 97 curated reaction event. The source record connects ATP, cob(II)alamin, FADH2 to PPP, AdoCbl, H+ in mitochondrial matrix.", "equation": "2 ATP + 2 cob(II)alamin + FADH2 → 2 PPP + 2 AdoCbl + 3 H+ + FAD", "inputs": [ { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-3149564", "reactome_id": "R-ALL-3149564", "label": "cob(II)alamin", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16304", "formula": "C62H88CoN13O14P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-3159274", "reactome_id": "R-ALL-3159274", "label": "PPP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15266", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-3159297", "reactome_id": "R-ALL-3159297", "label": "AdoCbl", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18408", "formula": "C72H100CoN18O17P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "corrinoid adenosyltransferase activity of 3xMMAB [mitochondrial matrix]", "activity_id": "3159301", "ec_numbers": [ "2.5.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:33797888", "title": "Redox-Linked Coordination Chemistry Directs Vitamin B 12 Trafficking", "journal": "Acc Chem Res", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33797888/" }, { "id": "PMID:30282455", "title": "Sacrificial Cobalt-Carbon Bond Homolysis in Coenzyme B 12 as a Cofactor Conservation Strategy", "journal": "J Am Chem Soc", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30282455/" }, { "id": "PMID:18251506", "title": "Functional characterization and mutation analysis of human ATP:Cob(I)alamin adenosyltransferase", "journal": "Biochemistry", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18251506/" }, { "id": "PMID:12438653", "title": "Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12438653/" }, { "id": "PMID:12514191", "title": "Identification of the human and bovine ATP:Cob(I)alamin adenosyltransferase cDNAs based on complementation of a bacterial mutant", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12514191/" }, { "id": "PMID:15913339", "title": "Spectroscopic evidence for the formation of a four-coordinate Co2+ cobalamin species upon binding to the human ATP:cobalamin adenosyltransferase", "journal": "J Am Chem Soc", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15913339/" } ], "x": 5948.666666666667, "y": 3043.565789473684, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3159259", "label": "MMAA:MUT binds AdoCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3159259", "summary": "MMAA:MUT binds AdoCbl is a Reactome release 97 curated reaction event. The source record connects 2xMMAA:2xMUT, AdoCbl to 2xMMAA:2xMUT:AdoCbl in mitochondrial matrix.", "equation": "2xMMAA:2xMUT + AdoCbl → 2xMMAA:2xMUT:AdoCbl", "inputs": [ { "node_id": "R-HSA-3159295", "reactome_id": "R-HSA-3159295", "label": "2xMMAA:2xMUT", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-3159297", "reactome_id": "R-ALL-3159297", "label": "AdoCbl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18408", "formula": "C72H100CoN18O17P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3159272", "reactome_id": "R-HSA-3159272", "label": "2xMMAA:2xMUT:AdoCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of 2xMMAA:2xMUT [mitochondrial matrix]", "activity_id": "9908941", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:2567699", "title": "Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction", "journal": "Genomics", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2567699/" }, { "id": "PMID:12438653", "title": "Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12438653/" }, { "id": "PMID:16281286", "title": "Spectrum of mutations in mut methylmalonic acidemia and identification of a common Hispanic mutation and haplotype", "journal": "Hum. Mutat.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16281286/" }, { "id": "PMID:15523652", "title": "Mutations in the MMAA gene in patients with the cblA disorder of vitamin B12 metabolism", "journal": "Hum. Mutat.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15523652/" } ], "x": 5670, "y": 3125.4605263157896, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3165230", "label": "SLC52A1,2,3 transport RIB from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · extracellular region · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:35015" ], "source_url": "https://reactome.org/content/detail/R-HSA-3165230", "summary": "SLC52A1,2,3 transport RIB from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects RIB to RIB in cytosol · extracellular region · plasma membrane and cross-references RHEA:35015.", "equation": "RIB → RIB", "inputs": [ { "node_id": "R-ALL-3165253", "reactome_id": "R-ALL-3165253", "label": "RIB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57986", "formula": "C17H19N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-196931", "reactome_id": "R-ALL-196931", "label": "RIB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57986", "formula": "C17H19N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "riboflavin transmembrane transporter activity of SLC52A1,2,3 [plasma membrane]", "activity_id": "3165235", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:20463145", "title": "Identification and comparative functional characterization of a new human riboflavin transporter hRFT3 expressed in the brain", "journal": "J. Nutr.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20463145/" }, { "id": "PMID:20206331", "title": "Brown-Vialetto-Van Laere syndrome, a ponto-bulbar palsy with deafness, is caused by mutations in c20orf54", "journal": "Am. J. Hum. Genet.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20206331/" }, { "id": "PMID:21110228", "title": "Brown-Vialetto-Van Laere and Fazio Londe syndrome is associated with a riboflavin transporter defect mimicking mild MADD: a new inborn error of metabolism with potential treatment", "journal": "J. Inherit. Metab. Dis.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21110228/" }, { "id": "PMID:18632736", "title": "Identification and functional characterization of a novel human and rat riboflavin transporter, RFT1", "journal": "Am. J. Physiol., Cell Physiol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18632736/" }, { "id": "PMID:24264046", "title": "Functional involvement of RFVT3/SLC52A3 in intestinal riboflavin absorption", "journal": "Am. J. Physiol. Gastrointest. Liver Physiol.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24264046/" } ], "x": 5670, "y": 3023.092105263158, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-3204311", "label": "AOX1 oxidises PXL to PDXate", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23725" ], "source_url": "https://reactome.org/content/detail/R-HSA-3204311", "summary": "AOX1 oxidises PXL to PDXate is a Reactome release 97 curated reaction event. The source record connects H2O, O2, PXL to PDXate, H2O2 in cytosol and cross-references RHEA:23725.", "equation": "H2O + O2 + PXL → PDXate + H2O2", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-965131", "reactome_id": "R-ALL-965131", "label": "PXL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17310", "formula": "C8H9NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-3204324", "reactome_id": "R-ALL-3204324", "label": "PDXate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30959", "formula": "C8H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aldehyde oxidase activity of 2xAOX1:cofactors [cytosol]", "activity_id": "3204329", "ec_numbers": [ "1.2.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:22279051", "title": "The impact of single nucleotide polymorphisms on human aldehyde oxidase", "journal": "Drug Metab. Dispos.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22279051/" } ], "x": 5670, "y": 2941.1973684210525, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3204318", "label": "cob(II)alamin is transferred from MMACHC:MMADHC:cob(II)alamin to MTRR:MTR", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3204318", "summary": "cob(II)alamin is transferred from MMACHC:MMADHC:cob(II)alamin to MTRR:MTR is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MTRR:MTR, MMACHC:MMADHC:cob(II)alamin to MTRR:MTR:cob(II)alamin, MMACHC, MMADHC in cytosol.", "equation": "MTRR:MTR + MMACHC:MMADHC:cob(II)alamin → MTRR:MTR:cob(II)alamin + MMACHC + MMADHC", "inputs": [ { "node_id": "R-HSA-3204322", "reactome_id": "R-HSA-3204322", "label": "MTRR:MTR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-3149533", "reactome_id": "R-HSA-3149533", "label": "MMACHC:MMADHC:cob(II)alamin", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3149544", "reactome_id": "R-HSA-3149544", "label": "MTRR:MTR:cob(II)alamin", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2980949", "reactome_id": "R-HSA-2980949", "label": "MMACHC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-3149529", "reactome_id": "R-HSA-3149529", "label": "MMADHC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of MTRR:MTR [cytosol]", "activity_id": "9908098", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:33797888", "title": "Redox-Linked Coordination Chemistry Directs Vitamin B 12 Trafficking", "journal": "Acc Chem Res", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33797888/" }, { "id": "PMID:22832074", "title": "Structural features of recombinant MMADHC isoforms and their interactions with MMACHC, proteins of mammalian vitamin B12 metabolism", "journal": "Mol. Genet. Metab.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22832074/" }, { "id": "PMID:23270877", "title": "Subcellular location of MMACHC and MMADHC, two human proteins central to intracellular vitamin B(12) metabolism", "journal": "Mol. Genet. Metab.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23270877/" }, { "id": "PMID:21071249", "title": "Interaction between MMACHC and MMADHC, two human proteins participating in intracellular vitamin B?? metabolism", "journal": "Mol. Genet. Metab.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21071249/" }, { "id": "PMID:19243433", "title": "Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase-methionine synthase reductase complex", "journal": "FEBS J.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19243433/" }, { "id": "PMID:17892308", "title": "Mechanism of coenzyme binding to human methionine synthase reductase revealed through the crystal structure of the FNR-like module and isothermal titration calorimetry", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17892308/" } ], "x": 5948.666666666667, "y": 3176.6447368421054, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-3245898", "label": "TCN1 binds correnoids in the circulation", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3245898", "summary": "TCN1 binds correnoids in the circulation is a Reactome release 97 curated reaction event. The source record connects TCN1, correnoid to TCN1:correnoid in extracellular region.", "equation": "TCN1 + correnoid → TCN1:correnoid", "inputs": [ { "node_id": "R-HSA-2980920", "reactome_id": "R-HSA-2980920", "label": "TCN1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9758893", "reactome_id": "R-ALL-9758893", "label": "correnoid", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33913", "formula": "C19CoN4R21", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9758894", "reactome_id": "R-HSA-9758894", "label": "TCN1:correnoid", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular sequestering activity of TCN1 [extracellular region]", "activity_id": "9763687", "ec_numbers": null, "accessions": [ "P20061" ] } ], "citations": [ { "id": "PMID:27411955", "title": "Structural basis of transcobalamin recognition by human CD320 receptor", "journal": "Nat Commun", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27411955/" }, { "id": "PMID:23846701", "title": "Structural basis for universal corrinoid recognition by the cobalamin transport protein haptocorrin", "journal": "J Biol Chem", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23846701/" }, { "id": "PMID:2777761", "title": "Structure of the cDNA encoding transcobalamin I, a neutrophil granule protein", "journal": "J. Biol. Chem.", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2777761/" }, { "id": "PMID:915005", "title": "Absorption, plasma transport, and cellular retention of cobalamin analogues in the rabbit. Evidence for the existence of multiple mechanisms that prevent the absorption and tissue dissemination of naturally occurring cobalamin analogues", "journal": "J Clin Invest", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/915005/" } ], "x": 5948.666666666667, "y": 3125.4605263157896, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3257122", "label": "SLC37A1, SLC37A2 exchange G6P for Pi across the ER membrane", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol · endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005829", "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:71537" ], "source_url": "https://reactome.org/content/detail/R-HSA-3257122", "summary": "SLC37A1, SLC37A2 exchange G6P for Pi across the ER membrane is a Reactome release 97 curated reaction event. The source record connects G6P, Pi to G6P, Pi in cytosol · endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:71537.", "equation": "G6P + Pi → G6P + Pi", "inputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113551", "reactome_id": "R-ALL-113551", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113782", "reactome_id": "R-ALL-113782", "label": "G6P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucose 6-phosphate:phosphate antiporter activity of SLC37A1,2 [endoplasmic reticulum membrane]", "activity_id": "3257127", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:21949678", "title": "SLC37A1 and SLC37A2 are phosphate-linked, glucose-6-phosphate antiporters", "journal": "PLoS ONE", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21949678/" }, { "id": "PMID:23506893", "title": "The SLC37 family of phosphate-linked sugar phosphate antiporters", "journal": "Mol. Aspects Med.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23506893/" } ], "x": 1251.3333333333333, "y": 554.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3262512", "label": "G6PC3 hydrolyzes G6P to form Glc and Pi (ubiquitous)", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:44905" ], "source_url": "https://reactome.org/content/detail/R-HSA-3262512", "summary": "G6PC3 hydrolyzes G6P to form Glc and Pi (ubiquitous) is a Reactome release 97 curated reaction event. The source record connects G6P, H2O to Pi, Glc in endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:44905.", "equation": "G6P + H2O → Pi + Glc", "inputs": [ { "node_id": "R-ALL-113782", "reactome_id": "R-ALL-113782", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113551", "reactome_id": "R-ALL-113551", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113780", "reactome_id": "R-ALL-113780", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucose-6-phosphatase activity of G6PC3 [endoplasmic reticulum membrane]", "activity_id": "3262503", "ec_numbers": [ "3.1.3.9" ], "accessions": [ "Q9BUM1" ] } ], "citations": [ { "id": "PMID:12965222", "title": "Identification and characterisation of a new human glucose-6-phosphatase isoform", "journal": "FEBS Lett", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12965222/" }, { "id": "PMID:14718531", "title": "Histidine 167 is the phosphate acceptor in glucose-6-phosphatase-beta forming a phosphohistidine enzyme intermediate during catalysis", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14718531/" }, { "id": "PMID:19118303", "title": "A syndrome with congenital neutropenia and mutations in G6PC3", "journal": "N. Engl. J. Med.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19118303/" } ], "x": 1530, "y": 554.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3266566", "label": "G6PC2 hydrolyzes G6P to form Glc and Pi (islet)", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:44905" ], "source_url": "https://reactome.org/content/detail/R-HSA-3266566", "summary": "G6PC2 hydrolyzes G6P to form Glc and Pi (islet) is a Reactome release 97 curated reaction event. The source record connects G6P, H2O to Pi, Glc in endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:44905.", "equation": "G6P + H2O → Pi + Glc", "inputs": [ { "node_id": "R-ALL-113782", "reactome_id": "R-ALL-113782", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113551", "reactome_id": "R-ALL-113551", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113780", "reactome_id": "R-ALL-113780", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucose-6-phosphatase activity of G6PC2 [endoplasmic reticulum membrane]", "activity_id": "3266569", "ec_numbers": [ "3.1.3.9" ], "accessions": [ "Q9NQR9" ] } ], "citations": [ { "id": "PMID:14722102", "title": "Enzymatic characterization of the pancreatic islet-specific glucose-6-phosphatase-related protein (IGRP)", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14722102/" } ], "x": 1808.6666666666667, "y": 554.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3301943", "label": "GSTK1 dimer transfers GS from GSH to CDNB", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:51221" ], "source_url": "https://reactome.org/content/detail/R-HSA-3301943", "summary": "GSTK1 dimer transfers GS from GSH to CDNB is a Reactome release 97 curated reaction event. The source record connects CDNB, GSH to DNPSG, HCl in mitochondrial matrix and cross-references RHEA:51221.", "equation": "CDNB + GSH → DNPSG + HCl", "inputs": [ { "node_id": "R-ALL-3301954", "reactome_id": "R-ALL-3301954", "label": "CDNB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:34718", "formula": "C6H3ClN2O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1655887", "reactome_id": "R-ALL-1655887", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16856", "formula": "C10H17N3O6S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-3301969", "reactome_id": "R-ALL-3301969", "label": "DNPSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133977", "formula": "C16H18N5O10S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-3301973", "reactome_id": "R-ALL-3301973", "label": "HCl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17883", "formula": "HCl", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione transferase activity of GSTK1 dimer [mitochondrial matrix]", "activity_id": "3301944", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:14742434", "title": "Gene and protein characterization of the human glutathione S-transferase kappa and evidence for a peroxisomal localization", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14742434/" } ], "x": 7740, "y": 2913.598039215686, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322001", "label": "GYS1 catalyzes the polyglucosylation of oligoGlc-GYG1", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18550" ], "source_url": "https://reactome.org/content/detail/R-HSA-3322001", "summary": "GYS1 catalyzes the polyglucosylation of oligoGlc-GYG1 is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, oligoGlc-GYG1:GYS1-a tetramer to UDP, polyGlc-GYG1:GYS1-a tetramer, H+ in cytosol and cross-references RHEA:18550.", "equation": "8 UDP-Glc + oligoGlc-GYG1:GYS1-a tetramer → 8 UDP + polyGlc-GYG1:GYS1-a tetramer + 8 H+", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322035", "reactome_id": "R-HSA-3322035", "label": "oligoGlc-GYG1:GYS1-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-110096", "reactome_id": "R-ALL-110096", "label": "UDP", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322054", "reactome_id": "R-HSA-3322054", "label": "polyGlc-GYG1:GYS1-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity of oligoGlc-GYG1:GYS1-a tetramer [cytosol]", "activity_id": "3322023", "ec_numbers": [ "2.4.1.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:19699667", "title": "Identification of a novel mutation in GYS1 (muscle-specific glycogen synthase) resulting in sudden cardiac death, that is diagnosable from skin fibroblasts", "journal": "Mol. Genet. Metab.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19699667/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1530, "y": 786.8846153846154, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322003", "label": "Autoglucosylation of GYG1 complexed with GYS1-b", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23361" ], "source_url": "https://reactome.org/content/detail/R-HSA-3322003", "summary": "Autoglucosylation of GYG1 complexed with GYS1-b is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, GYG1:GYS1-b tetramer to UDP, oligoGlc-GYG1:GYS1-b tetramer, H+ in cytosol and cross-references RHEA:23361.", "equation": "8 UDP-Glc + GYG1:GYS1-b tetramer → 8 UDP + oligoGlc-GYG1:GYS1-b tetramer + 8 H+", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322062", "reactome_id": "R-HSA-3322062", "label": "GYG1:GYS1-b tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-110096", "reactome_id": "R-ALL-110096", "label": "UDP", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322053", "reactome_id": "R-HSA-3322053", "label": "oligoGlc-GYG1:GYS1-b tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycogenin glucosyltransferase activity of GYG1:GYS1-b tetramer [cytosol]", "activity_id": "3322038", "ec_numbers": [ "2.4.1.186" ], "accessions": [] } ], "citations": [ { "id": "PMID:20357282", "title": "Glycogenin-1 deficiency and inactivated priming of glycogen synthesis", "journal": "N. Engl. J. Med.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20357282/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1808.6666666666667, "y": 786.8846153846154, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322005", "label": "GBE1 catalyzes branch formation in polyGlc-GYG1 complexed with GYS1-a", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3322005", "summary": "GBE1 catalyzes branch formation in polyGlc-GYG1 complexed with GYS1-a is a Reactome release 97 curated reaction event. The source record connects polyGlc-GYG1:GYS1-a tetramer to glycogen-GYG1:GYS1-a tetramer in cytosol.", "equation": "polyGlc-GYG1:GYS1-a tetramer → glycogen-GYG1:GYS1-a tetramer", "inputs": [ { "node_id": "R-HSA-3322054", "reactome_id": "R-HSA-3322054", "label": "polyGlc-GYG1:GYS1-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3322059", "reactome_id": "R-HSA-3322059", "label": "glycogen-GYG1:GYS1-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "1,4-alpha-glucan branching enzyme activity of GBE1 [cytosol]", "activity_id": "71577", "ec_numbers": [ "2.4.1.18" ], "accessions": [ "Q04446" ] } ], "citations": [ { "id": "PMID:8613547", "title": "Hepatic and neuromuscular forms of glycogen storage disease type IV caused by mutations in the same glycogen-branching enzyme gene.", "journal": "J Clin Invest", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8613547/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1808.6666666666667, "y": 996.3461538461538, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322009", "label": "GYS2 catalyzes the polyglucosylation of oligoGlc-GYG2", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18550" ], "source_url": "https://reactome.org/content/detail/R-HSA-3322009", "summary": "GYS2 catalyzes the polyglucosylation of oligoGlc-GYG2 is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, oligoGlc-GYG2:GYS2-a tetramer to UDP, polyGlc-GYG2:GYS2-a tetramer, H+ in cytosol and cross-references RHEA:18550.", "equation": "8 UDP-Glc + oligoGlc-GYG2:GYS2-a tetramer → 8 UDP + polyGlc-GYG2:GYS2-a tetramer + 8 H+", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3321999", "reactome_id": "R-HSA-3321999", "label": "oligoGlc-GYG2:GYS2-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-110096", "reactome_id": "R-ALL-110096", "label": "UDP", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322056", "reactome_id": "R-HSA-3322056", "label": "polyGlc-GYG2:GYS2-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity of oligoGlc-GYG2:GYS2-a tetramer [cytosol]", "activity_id": "3322006", "ec_numbers": [ "2.4.1.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:9691087", "title": "Mutations in the liver glycogen synthase gene in children with hypoglycemia due to glycogen storage disease type 0", "journal": "J Clin Invest", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9691087/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1251.3333333333333, "y": 816.8076923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322014", "label": "Autoglucosylation of GYG2 complexed with GYS2-a", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23361" ], "source_url": "https://reactome.org/content/detail/R-HSA-3322014", "summary": "Autoglucosylation of GYG2 complexed with GYS2-a is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, GYG2:GYS2-a tetramer to UDP, oligoGlc-GYG2:GYS2-a tetramer, H+ in cytosol and cross-references RHEA:23361.", "equation": "8 UDP-Glc + GYG2:GYS2-a tetramer → 8 UDP + oligoGlc-GYG2:GYS2-a tetramer + 8 H+", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322031", "reactome_id": "R-HSA-3322031", "label": "GYG2:GYS2-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-110096", "reactome_id": "R-ALL-110096", "label": "UDP", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3321999", "reactome_id": "R-HSA-3321999", "label": "oligoGlc-GYG2:GYS2-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycogenin glucosyltransferase activity of GYG2:GYS2-a tetramer [cytosol]", "activity_id": "3322010", "ec_numbers": [ "2.4.1.186" ], "accessions": [] } ], "citations": [ { "id": "PMID:9346895", "title": "Glycogenin-2, a novel self-glucosylating protein involved in liver glycogen biosynthesis.", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9346895/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1530, "y": 816.8076923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322016", "label": "GBE1 catalyzes branch formation in polyGlc-GYG2 complexed with GYS2-a", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3322016", "summary": "GBE1 catalyzes branch formation in polyGlc-GYG2 complexed with GYS2-a is a Reactome release 97 curated reaction event. The source record connects polyGlc-GYG2:GYS2-a tetramer to glycogen-GYG2:GYS2-a tetramer in cytosol.", "equation": "polyGlc-GYG2:GYS2-a tetramer → glycogen-GYG2:GYS2-a tetramer", "inputs": [ { "node_id": "R-HSA-3322056", "reactome_id": "R-HSA-3322056", "label": "polyGlc-GYG2:GYS2-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3322011", "reactome_id": "R-HSA-3322011", "label": "glycogen-GYG2:GYS2-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "1,4-alpha-glucan branching enzyme activity of GBE1 [cytosol]", "activity_id": "71577", "ec_numbers": [ "2.4.1.18" ], "accessions": [ "Q04446" ] } ], "citations": [ { "id": "PMID:8613547", "title": "Hepatic and neuromuscular forms of glycogen storage disease type IV caused by mutations in the same glycogen-branching enzyme gene.", "journal": "J Clin Invest", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8613547/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1251.3333333333333, "y": 1026.2692307692307, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322019", "label": "Autoglucosylation of GYG2 complexed with GYS2-b", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23361" ], "source_url": "https://reactome.org/content/detail/R-HSA-3322019", "summary": "Autoglucosylation of GYG2 complexed with GYS2-b is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, GYG2:GYS2-b tetramer to UDP, oligoGlc-GYG2:GYS2-b tetramer, H+ in cytosol and cross-references RHEA:23361.", "equation": "8 UDP-Glc + GYG2:GYS2-b tetramer → 8 UDP + oligoGlc-GYG2:GYS2-b tetramer + 8 H+", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322065", "reactome_id": "R-HSA-3322065", "label": "GYG2:GYS2-b tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-110096", "reactome_id": "R-ALL-110096", "label": "UDP", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322007", "reactome_id": "R-HSA-3322007", "label": "oligoGlc-GYG2:GYS2-b tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycogenin glucosyltransferase activity of GYG2:GYS2-b tetramer [cytosol]", "activity_id": "3322066", "ec_numbers": [ "2.4.1.186" ], "accessions": [] } ], "citations": [ { "id": "PMID:9346895", "title": "Glycogenin-2, a novel self-glucosylating protein involved in liver glycogen biosynthesis.", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9346895/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1808.6666666666667, "y": 816.8076923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322025", "label": "Autoglucosylation of GYG1 complexed with GYS1-a", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23361" ], "source_url": "https://reactome.org/content/detail/R-HSA-3322025", "summary": "Autoglucosylation of GYG1 complexed with GYS1-a is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, GYG1:GYS1-a tetramer to UDP, oligoGlc-GYG1:GYS1-a tetramer, H+ in cytosol and cross-references RHEA:23361.", "equation": "8 UDP-Glc + GYG1:GYS1-a tetramer → 8 UDP + oligoGlc-GYG1:GYS1-a tetramer + 8 H+", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322050", "reactome_id": "R-HSA-3322050", "label": "GYG1:GYS1-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-110096", "reactome_id": "R-ALL-110096", "label": "UDP", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322035", "reactome_id": "R-HSA-3322035", "label": "oligoGlc-GYG1:GYS1-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycogenin glucosyltransferase activity of GYG1:GYS1-a tetramer [cytosol]", "activity_id": "3322020", "ec_numbers": [ "2.4.1.186" ], "accessions": [] } ], "citations": [ { "id": "PMID:20357282", "title": "Glycogenin-1 deficiency and inactivated priming of glycogen synthesis", "journal": "N. Engl. J. Med.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20357282/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1251.3333333333333, "y": 846.7307692307693, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322041", "label": "Phosphorylated GYS1 catalyzes the polyglucosylation of oligoGlc-GYG1", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18550" ], "source_url": "https://reactome.org/content/detail/R-HSA-3322041", "summary": "Phosphorylated GYS1 catalyzes the polyglucosylation of oligoGlc-GYG1 is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, oligoGlc-GYG1:GYS1-b tetramer to UDP, polyGlc-GYG1:GYS1-b tetramer, H+ in cytosol and cross-references RHEA:18550.", "equation": "8 UDP-Glc + oligoGlc-GYG1:GYS1-b tetramer → 8 UDP + polyGlc-GYG1:GYS1-b tetramer + 8 H+", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322053", "reactome_id": "R-HSA-3322053", "label": "oligoGlc-GYG1:GYS1-b tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-110096", "reactome_id": "R-ALL-110096", "label": "UDP", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-3322063", "reactome_id": "R-HSA-3322063", "label": "polyGlc-GYG1:GYS1-b tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alpha-1,4-glucan glucosyltransferase (UDP-glucose donor) activity of oligoGlc-GYG1:GYS1-b tetramer [cytosol]", "activity_id": "3322055", "ec_numbers": [ "2.4.1.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:19699667", "title": "Identification of a novel mutation in GYS1 (muscle-specific glycogen synthase) resulting in sudden cardiac death, that is diagnosable from skin fibroblasts", "journal": "Mol. Genet. Metab.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19699667/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1530, "y": 846.7307692307693, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3322057", "label": "GBE1 catalyzes branch formation in polyGlc-GYG1 complexed with GYS1-b", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3322057", "summary": "GBE1 catalyzes branch formation in polyGlc-GYG1 complexed with GYS1-b is a Reactome release 97 curated reaction event. The source record connects polyGlc-GYG1:GYS1-b tetramer to glycogen-GYG1:GYS1-b tetramer in cytosol.", "equation": "polyGlc-GYG1:GYS1-b tetramer → glycogen-GYG1:GYS1-b tetramer", "inputs": [ { "node_id": "R-HSA-3322063", "reactome_id": "R-HSA-3322063", "label": "polyGlc-GYG1:GYS1-b tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3322021", "reactome_id": "R-HSA-3322021", "label": "glycogen-GYG1:GYS1-b tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "1,4-alpha-glucan branching enzyme activity of GBE1 [cytosol]", "activity_id": "71577", "ec_numbers": [ "2.4.1.18" ], "accessions": [ "Q04446" ] } ], "citations": [ { "id": "PMID:8613547", "title": "Hepatic and neuromuscular forms of glycogen storage disease type IV caused by mutations in the same glycogen-branching enzyme gene.", "journal": "J Clin Invest", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8613547/" }, { "id": "PMID:22248338", "title": "Glycogen and its metabolism: some new developments and old themes", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248338/" } ], "x": 1530, "y": 1026.2692307692307, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3323111", "label": "Cytosolic carboxylases translocate to mitochondrial matrix", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3323111", "summary": "Cytosolic carboxylases translocate to mitochondrial matrix is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects hCBXs to hCBXs in cytosol · mitochondrial matrix.", "equation": "hCBXs → hCBXs", "inputs": [ { "node_id": "R-HSA-3323101", "reactome_id": "R-HSA-3323101", "label": "hCBXs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3065950", "reactome_id": "R-HSA-3065950", "label": "hCBXs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20153287", "title": "Holocarboxylase synthetase: correlation of protein localisation with biological function", "journal": "Arch. Biochem. Biophys.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20153287/" }, { "id": "PMID:9630604", "title": "Identification of holocarboxylase synthetase (HCS) proteins in human placenta", "journal": "Biochim. Biophys. Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9630604/" }, { "id": "PMID:22123817", "title": "Selectivity in post-translational biotin addition to five human carboxylases", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22123817/" } ], "x": 5948.666666666667, "y": 3186.8815789473683, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-350147", "label": "VD3 translocates from extracellular region to ER membrane", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum membrane · extracellular region", "compartment_ids": [ "GO:0005789", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-350147", "summary": "VD3 translocates from extracellular region to ER membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects VD3 to VD3 in endoplasmic reticulum membrane · extracellular region.", "equation": "VD3 → VD3", "inputs": [ { "node_id": "R-ALL-209767", "reactome_id": "R-ALL-209767", "label": "VD3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28940", "formula": "C27H44O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8963870", "reactome_id": "R-ALL-8963870", "label": "VD3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28940", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:15465040", "title": "Metabolism of vitamin D by human microsomal CYP2R1", "journal": "Biochem Biophys Res Commun", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15465040/" } ], "x": 4451.333333333333, "y": 1860.1136363636363, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-350158", "label": "LGMN hydrolyzes GC, releasing CUBN and 25(OH)D", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-350158", "summary": "LGMN hydrolyzes GC, releasing CUBN and 25(OH)D is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CUBN:GC:25(OH)D to CUBN, 25(OH)D in lysosomal lumen.", "equation": "CUBN:GC:25(OH)D → CUBN + 25(OH)D", "inputs": [ { "node_id": "R-HSA-350085", "reactome_id": "R-HSA-350085", "label": "CUBN:GC:25(OH)D", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-350088", "reactome_id": "R-HSA-350088", "label": "CUBN", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-350136", "reactome_id": "R-ALL-350136", "label": "25(OH)D", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17933", "formula": "C27H44O2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cysteine-type endopeptidase activity of LGMN [lysosomal lumen]", "activity_id": "350178", "ec_numbers": [ "3.4.22.1" ], "accessions": [ "Q99538" ] } ], "citations": [ { "id": "PMID:10052453", "title": "An endocytic pathway essential for renal uptake and activation of the steroid 25-(OH) vitamin D3", "journal": "Cell", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10052453/" } ], "x": 4451.333333333333, "y": 1836.5378787878788, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-350168", "label": "LRP2-mediated uptake of extracellular CUBN:GC:25(OH)D", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "plasma membrane · extracellular region · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005576", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-350168", "summary": "LRP2-mediated uptake of extracellular CUBN:GC:25(OH)D is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CUBN:GC:25(OH)D to CUBN:GC:25(OH)D in plasma membrane · extracellular region · cytosol.", "equation": "CUBN:GC:25(OH)D → CUBN:GC:25(OH)D", "inputs": [ { "node_id": "R-HSA-350115", "reactome_id": "R-HSA-350115", "label": "CUBN:GC:25(OH)D", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-350092", "reactome_id": "R-HSA-350092", "label": "CUBN:GC:25(OH)D", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of LRP2:LDLRAP1 [plasma membrane]", "activity_id": "350169", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8706697", "title": "Cloning and sequencing of human gp330, a Ca(2+)-binding receptor with potential intracellular signaling properties", "journal": "Eur J Biochem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8706697/" }, { "id": "PMID:7768901", "title": "Identification of glycoprotein 330 as an endocytic receptor for apolipoprotein J/clusterin", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7768901/" }, { "id": "PMID:10052453", "title": "An endocytic pathway essential for renal uptake and activation of the steroid 25-(OH) vitamin D3", "journal": "Cell", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10052453/" }, { "id": "PMID:21595846", "title": "Role of megalin and cubilin in the metabolism of vitamin D(3)", "journal": "Ther Apher Dial", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21595846/" } ], "x": 5008.666666666667, "y": 1801.1742424242425, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-350186", "label": "CUBN binds GC:25(OH)D", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-350186", "summary": "CUBN binds GC:25(OH)D is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects GC:25(OH)D, CUBN to CUBN:GC:25(OH)D in extracellular region · plasma membrane.", "equation": "GC:25(OH)D + CUBN → CUBN:GC:25(OH)D", "inputs": [ { "node_id": "R-HSA-209892", "reactome_id": "R-HSA-209892", "label": "GC:25(OH)D", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-264843", "reactome_id": "R-HSA-264843", "label": "CUBN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-350115", "reactome_id": "R-HSA-350115", "label": "CUBN:GC:25(OH)D", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of CUBN [plasma membrane]", "activity_id": "9959975", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11717447", "title": "Cubilin dysfunction causes abnormal metabolism of the steroid hormone 25(OH) vitamin D(3)", "journal": "Proc Natl Acad Sci U S A", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11717447/" } ], "x": 4451.333333333333, "y": 1807.0681818181818, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-352232", "label": "SLC7A5:SLC3A2 transports neutral amino acids from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:73011", "RHEA:70947", "RHEA:72807", "RHEA:70943", "RHEA:68572", "RHEA:70719", "RHEA:27950", "RHEA:70939", "RHEA:29703" ], "source_url": "https://reactome.org/content/detail/R-HSA-352232", "summary": "SLC7A5:SLC3A2 transports neutral amino acids from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ligands of SLC7A5 to ligands of SLC7A5 in plasma membrane and cross-references RHEA:73011, RHEA:70947, RHEA:72807, RHEA:70943, RHEA:68572, RHEA:70719, RHEA:27950, RHEA:70939, RHEA:29703.", "equation": "ligands of SLC7A5 → ligands of SLC7A5", "inputs": [ { "node_id": "R-ALL-352229", "reactome_id": "R-ALL-352229", "label": "ligands of SLC7A5", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-352227", "reactome_id": "R-ALL-352227", "label": "ligands of SLC7A5", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "L-amino acid transmembrane transporter activity of SLC7A5:SLC3A2 [plasma membrane]", "activity_id": "352233", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10049700", "title": "Human LAT1, a subunit of system L amino acid transporter: molecular cloning and transport function", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10049700/" }, { "id": "PMID:10391915", "title": "Identification of a membrane protein, LAT-2, that Co-expresses with 4F2 heavy chain, an L-type amino acid transport activity with broad specificity for small and large zwitterionic amino acids", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10391915/" }, { "id": "PMID:18262359", "title": "Functional characterization of tyrosine transport in fibroblast cells from healthy controls", "journal": "Neurosci Lett", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18262359/" } ], "x": 7461.333333333333, "y": 1784.9583333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-372342", "label": "MPC1:MPC2 imports PYR, H+ to mitochondrial matrix", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial intermembrane space · mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005758", "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:64720", "RHEA:64721" ], "source_url": "https://reactome.org/content/detail/R-HSA-372342", "summary": "MPC1:MPC2 imports PYR, H+ to mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H+, PYR to H+, PYR in mitochondrial intermembrane space · mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:64720, RHEA:64721.", "equation": "H+ + PYR → H+ + PYR", "inputs": [ { "node_id": "R-ALL-163953", "reactome_id": "R-ALL-163953", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9012375", "reactome_id": "R-ALL-9012375", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pyruvate transmembrane transporter activity of MPC dimer [mitochondrial inner membrane]", "activity_id": "372338", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:22628558", "title": "A mitochondrial pyruvate carrier required for pyruvate uptake in yeast, Drosophila, and humans", "journal": "Science", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22628558/" }, { "id": "PMID:4822737", "title": "Specific inhibition of pyruvate transport in rat liver mitochondria and human erythrocytes by alpha-cyano-4-hydroxycinnamate", "journal": "Biochem. J.", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4822737/" }, { "id": "PMID:11945601", "title": "The transport of pyruvate in rat liver mitochondria", "journal": "FEBS Lett", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/11945601/" }, { "id": "PMID:27317664", "title": "MPC1-like Is a Placental Mammal-specific Mitochondrial Pyruvate Carrier Subunit Expressed in Postmeiotic Male Germ Cells", "journal": "J Biol Chem", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27317664/" }, { "id": "PMID:33227948", "title": "Insights on the Quest for the Structure-Function Relationship of the Mitochondrial Pyruvate Carrier", "journal": "Biology (Basel)", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33227948/" }, { "id": "PMID:32403431", "title": "Characteristic Analysis of Homo- and Heterodimeric Complexes of Human Mitochondrial Pyruvate Carrier Related to Metabolic Diseases", "journal": "Int J Mol Sci", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32403431/" } ], "x": 4451.333333333333, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-372448", "label": "SLC25A12,13 exchange L-Glu and L-Asp", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "electron-transport", "malate-aspartate-shuttle" ], "compartment": "cytosol · mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:70783" ], "source_url": "https://reactome.org/content/detail/R-HSA-372448", "summary": "SLC25A12,13 exchange L-Glu and L-Asp is a Reactome release 97 curated reaction event. The source record connects L-Asp, L-Glu, H+ to L-Asp, L-Glu, H+ in cytosol · mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:70783.", "equation": "L-Asp + L-Glu + H+ → L-Asp + L-Glu + H+", "inputs": [ { "node_id": "R-ALL-113553", "reactome_id": "R-ALL-113553", "label": "L-Asp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29448", "reactome_id": "R-ALL-29448", "label": "L-Asp", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acidic amino acid transmembrane transporter activity of SLC25A12,13 [mitochondrial inner membrane]", "activity_id": "372473", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11566871", "title": "Citrin and aralar1 are Ca(2+)-stimulated aspartate/glutamate transporters in mitochondria", "journal": "EMBO J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11566871/" }, { "id": "PMID:10642534", "title": "Characterization of a second member of the subfamily of calcium-binding mitochondrial carriers expressed in human non-excitable tissues", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10642534/" }, { "id": "PMID:10369257", "title": "The gene mutated in adult-onset type II citrullinaemia encodes a putative mitochondrial carrier protein", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10369257/" }, { "id": "PMID:12602510", "title": "Pathogenesis and pathophysiology of citrin (a mitochondrial aspartate glutamate carrier) deficiency", "journal": "Metab Brain Dis", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12602510/" }, { "id": "PMID:36967723", "title": "Exogenous aralar/slc25a12 can replace citrin/slc25a13 as malate aspartate shuttle component in liver", "journal": "Mol Genet Metab Rep", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36967723/" }, { "id": "PMID:32740958", "title": "Metabolic basis and treatment of citrin deficiency", "journal": "J Inherit Metab Dis", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/32740958/" } ], "x": 6521.333333333333, "y": 457.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-372480", "label": "2-oxoglutarate [mitochondrial matrix] + 2-oxoadipate [cytosol] 2-oxoglutarate [cytosol] + 2-oxoadipate [mitochondrial matrix]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane · cytosol", "compartment_ids": [ "GO:0005759", "GO:0005743", "GO:0005829" ], "rhea_ids": [ "RHEA:71739" ], "source_url": "https://reactome.org/content/detail/R-HSA-372480", "summary": "2-oxoglutarate [mitochondrial matrix] + 2-oxoadipate [cytosol] 2-oxoglutarate [cytosol] + 2-oxoadipate [mitochondrial matrix] is a Reactome release 97 curated reaction event. The source record connects 2OG, 2-OA to 2OG, 2-OA in mitochondrial matrix · mitochondrial inner membrane · cytosol and cross-references RHEA:71739.", "equation": "2OG + 2-OA → 2OG + 2-OA", "inputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113577", "reactome_id": "R-ALL-113577", "label": "2-OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57499", "formula": "C6H6O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70951", "reactome_id": "R-ALL-70951", "label": "2-OA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57499", "formula": "C6H6O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alpha-ketoglutarate transmembrane transporter activity of SLC25A21 [mitochondrial inner membrane]", "activity_id": "372492", "ec_numbers": null, "accessions": [ "Q9BQT8" ] } ], "citations": [ { "id": "PMID:11083877", "title": "Identification of the human mitochondrial oxodicarboxylate carrier. Bacterial expression, reconstitution, functional characterization, tissue distribution, and chromosomal location", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11083877/" } ], "x": 7078.666666666667, "y": 2074.2916666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-372519", "label": "AcCho is hydrolyzed to Cho and acetate by ACHE", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:17562" ], "source_url": "https://reactome.org/content/detail/R-HSA-372519", "summary": "AcCho is hydrolyzed to Cho and acetate by ACHE is a Reactome release 97 curated reaction event. The source record connects AcCho, H2O to Cho, acetate in extracellular region · plasma membrane and cross-references RHEA:17562.", "equation": "AcCho + H2O → Cho + acetate", "inputs": [ { "node_id": "R-ALL-372533", "reactome_id": "R-ALL-372533", "label": "AcCho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15355", "formula": "C7H16NO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-264638", "reactome_id": "R-ALL-264638", "label": "Cho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524044", "reactome_id": "R-ALL-1524044", "label": "acetate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetylcholinesterase activity of Cholinesterase [plasma membrane]", "activity_id": "1524052", "ec_numbers": [ "3.1.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:18457821", "title": "Rational design of a drug for Alzheimer's disease with cholinesterase inhibitory and neuroprotective activity", "journal": "Chem Biol Interact", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18457821/" }, { "id": "PMID:1748670", "title": "The effect of elimination of intersubunit disulfide bonds on the activity, assembly, and secretion of recombinant human acetylcholinesterase. Expression of acetylcholinesterase Cys-580----Ala mutant", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1748670/" }, { "id": "PMID:11053835", "title": "Structures of recombinant native and E202Q mutant human acetylcholinesterase complexed with the snake-venom toxin fasciculin-II", "journal": "Acta Crystallogr D Biol Crystallogr", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11053835/" } ], "x": 2660, "y": 1636.9109589041095, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-372819", "label": "PCK2 phosphorylates OA to yield PEP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:10389" ], "source_url": "https://reactome.org/content/detail/R-HSA-372819", "summary": "PCK2 phosphorylates OA to yield PEP is a Reactome release 97 curated reaction event. The source record connects OA, GTP to PEP, CO2, GDP in mitochondrial matrix and cross-references RHEA:10389.", "equation": "OA + GTP → PEP + CO2 + GDP", "inputs": [ { "node_id": "R-ALL-113587", "reactome_id": "R-ALL-113587", "label": "OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113573", "reactome_id": "R-ALL-113573", "label": "GTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37565", "formula": "C10H12N5O14P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-372364", "reactome_id": "R-ALL-372364", "label": "PEP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58702", "formula": "C3H2O6P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113525", "reactome_id": "R-ALL-113525", "label": "GDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58189", "formula": "C10H12N5O11P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoenolpyruvate carboxykinase (GTP) activity of PCK2 [mitochondrial matrix]", "activity_id": "372821", "ec_numbers": [ "4.1.1.32" ], "accessions": [] } ], "citations": [ { "id": "PMID:9657976", "title": "Human mitochondrial phosphoenolpyruvate carboxykinase 2 gene. Structure, chromosomal localization and tissue-specific expression", "journal": "Biochem J", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9657976/" }, { "id": "PMID:8645161", "title": "Molecular cloning, sequencing and expression of the cDNA of the mitochondrial form of phosphoenolpyruvate carboxykinase from human liver", "journal": "Biochem J", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8645161/" } ], "x": 1808.6666666666667, "y": 295.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-376851", "label": "SLC25A11 exchanges 2-OG and MAL", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport", "malate-aspartate-shuttle" ], "compartment": "cytosol · mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:71587", "RHEA:71589" ], "source_url": "https://reactome.org/content/detail/R-HSA-376851", "summary": "SLC25A11 exchanges 2-OG and MAL is a Reactome release 97 curated reaction event. The source record connects MAL, 2OG to MAL, 2OG in cytosol · mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:71587, RHEA:71589.", "equation": "MAL + 2OG → MAL + 2OG", "inputs": [ { "node_id": "R-ALL-198498", "reactome_id": "R-ALL-198498", "label": "MAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113544", "reactome_id": "R-ALL-113544", "label": "MAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxoglutarate:malate antiporter activity of SLC25A11 homodimer [mitochondrial inner membrane]", "activity_id": "198502", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:16920706", "title": "Porphyrin accumulation in mitochondria is mediated by 2-oxoglutarate carrier", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16920706/" }, { "id": "PMID:8597574", "title": "The formation of a disulfide cross-link between the two subunits demonstrates the dimeric structure of the mitochondrial oxoglutarate carrier", "journal": "Biochim Biophys Acta", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8597574/" }, { "id": "PMID:4687397", "title": "Mechanism of the exchanges catalysed by the oxoglutarate translocator of rat-heart mitochondria. Kinetics of the external-product inhibition", "journal": "Eur J Biochem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4687397/" }, { "id": "PMID:25637873", "title": "The mitochondrial dicarboxylate and 2-oxoglutarate carriers do not transport glutathione", "journal": "FEBS Lett", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25637873/" }, { "id": "PMID:29431636", "title": "Germline Mutations in the Mitochondrial 2-Oxoglutarate/Malate Carrier SLC25A11 Gene Confer a Predisposition to Metastatic Paragangliomas", "journal": "Cancer Res", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29431636/" } ], "x": 4730, "y": 754.9666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3780994", "label": "GYS2 catalyzes the incorporation of phosphoglucose into glycogen-GYG2", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3780994", "summary": "GYS2 catalyzes the incorporation of phosphoglucose into glycogen-GYG2 is a Reactome release 97 curated reaction event. The source record connects PPP1R3C:glycogen-GYG2:GYS2-a tetramer, UDP-Glc to PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer, UMP, H+ in cytosol.", "equation": "PPP1R3C:glycogen-GYG2:GYS2-a tetramer + UDP-Glc → PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer + UMP + H+", "inputs": [ { "node_id": "R-HSA-3777104", "reactome_id": "R-HSA-3777104", "label": "PPP1R3C:glycogen-GYG2:GYS2-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3777094", "reactome_id": "R-HSA-3777094", "label": "PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-109402", "reactome_id": "R-ALL-109402", "label": "UMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57865", "formula": "C9H11N2O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycogen synthase activity, transferring glucose-1-phosphate of PPP1R3C:glycogen-GYG2:GYS2-a tetramer [cytosol]", "activity_id": "3781006", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25416783", "title": "Glycogen phosphomonoester distribution in mouse models of the progressive myoclonic epilepsy, Lafora disease", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25416783/" }, { "id": "PMID:26216881", "title": "Muscle Glycogen Remodeling and Glycogen Phosphate Metabolism following Exhaustive Exercise of Wild Type and Laforin Knockout Mice", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26216881/" }, { "id": "PMID:23663739", "title": "Hyperphosphorylation of glucosyl C6 carbons and altered structure of glycogen in the neurodegenerative epilepsy Lafora disease", "journal": "Cell Metab.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23663739/" }, { "id": "PMID:21356517", "title": "Phosphate incorporation during glycogen synthesis and Lafora disease", "journal": "Cell Metab.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21356517/" }, { "id": "PMID:18070875", "title": "Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG)", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18070875/" } ], "x": 1808.6666666666667, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3780995", "label": "NHLRC1 mediated ubiquitination of EPM2A (laforin) and PPP1RC3 (PTG) associated with glycogen-GYG2", "schema_class": "BlackBoxEvent", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3780995", "summary": "NHLRC1 mediated ubiquitination of EPM2A (laforin) and PPP1RC3 (PTG) associated with glycogen-GYG2 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects EPM2A:PPP1R3C:glycogen-GYG2:GYS2-a tetramer, Ub to K48polyUb-PPP1R3C, glycogen-GYG2:GYS2-a tetramer, K48polyUb-EPM2A in cytosol.", "equation": "EPM2A:PPP1R3C:glycogen-GYG2:GYS2-a tetramer + 2 Ub → K48polyUb-PPP1R3C + glycogen-GYG2:GYS2-a tetramer + 2 K48polyUb-EPM2A", "inputs": [ { "node_id": "R-HSA-3780991", "reactome_id": "R-HSA-3780991", "label": "EPM2A:PPP1R3C:glycogen-GYG2:GYS2-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-113595", "reactome_id": "R-HSA-113595", "label": "Ub", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3781005", "reactome_id": "R-HSA-3781005", "label": "K48polyUb-PPP1R3C", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-3322011", "reactome_id": "R-HSA-3322011", "label": "glycogen-GYG2:GYS2-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-3781004", "reactome_id": "R-HSA-3781004", "label": "K48polyUb-EPM2A", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "ubiquitin-protein transferase activity of NHLRC1 [cytosol]", "activity_id": "3781003", "ec_numbers": [ "6.3.2.19" ], "accessions": [ "Q6VVB1" ] } ], "citations": [ { "id": "PMID:20538597", "title": "Genetic depletion of the malin E3 ubiquitin ligase in mice leads to lafora bodies and the accumulation of insoluble laforin", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20538597/" }, { "id": "PMID:15930137", "title": "Insights into Lafora disease: malin is an E3 ubiquitin ligase that ubiquitinates and promotes the degradation of laforin", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15930137/" }, { "id": "PMID:18070875", "title": "Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG)", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18070875/" } ], "x": 1251.3333333333333, "y": 1116.0384615384614, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-3780997", "label": "PPP1R3C binds to glycogen:GYG2:GYS2", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3780997", "summary": "PPP1R3C binds to glycogen:GYG2:GYS2 is a Reactome release 97 curated reaction event. The source record connects PPP1R3C, glycogen-GYG2:GYS2-a tetramer to PPP1R3C:glycogen-GYG2:GYS2-a tetramer in cytosol.", "equation": "PPP1R3C + glycogen-GYG2:GYS2-a tetramer → PPP1R3C:glycogen-GYG2:GYS2-a tetramer", "inputs": [ { "node_id": "R-HSA-3777102", "reactome_id": "R-HSA-3777102", "label": "PPP1R3C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-3322011", "reactome_id": "R-HSA-3322011", "label": "glycogen-GYG2:GYS2-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3777104", "reactome_id": "R-HSA-3777104", "label": "PPP1R3C:glycogen-GYG2:GYS2-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein-macromolecule adaptor activity of PPP1R3C [cytosol]", "activity_id": "9931455", "ec_numbers": null, "accessions": [ "Q9UQK1" ] } ], "citations": [ { "id": "PMID:18070875", "title": "Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG)", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18070875/" } ], "x": 1808.6666666666667, "y": 1026.2692307692307, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3781001", "label": "EPM2A dimer binds PPP1R3C:phosphoglycogen-GYG1 complex", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3781001", "summary": "EPM2A dimer binds PPP1R3C:phosphoglycogen-GYG1 complex is a Reactome release 97 curated reaction event. The source record connects PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer, EPM2A dimer to EPM2A:PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer in cytosol.", "equation": "PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer + EPM2A dimer → EPM2A:PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer", "inputs": [ { "node_id": "R-HSA-3777093", "reactome_id": "R-HSA-3777093", "label": "PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-6805697", "reactome_id": "R-HSA-6805697", "label": "EPM2A dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3780999", "reactome_id": "R-HSA-3780999", "label": "EPM2A:PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein-macromolecule adaptor activity of PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer [cytosol]", "activity_id": "9931456", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18070875", "title": "Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG)", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18070875/" } ], "x": 1251.3333333333333, "y": 1056.1923076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3781009", "label": "NHLRC1 mediated ubiquitination of EPM2A and PPP1RC3 associated with glycogen-GYG1", "schema_class": "BlackBoxEvent", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3781009", "summary": "NHLRC1 mediated ubiquitination of EPM2A and PPP1RC3 associated with glycogen-GYG1 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects EPM2A:PPP1R3C:glycogen-GYG1:GYS1-a tetramer, Ub to K48polyUb-PPP1R3C, glycogen-GYG1:GYS1-a tetramer, K48polyUb-EPM2A in cytosol.", "equation": "EPM2A:PPP1R3C:glycogen-GYG1:GYS1-a tetramer + 2 Ub → K48polyUb-PPP1R3C + glycogen-GYG1:GYS1-a tetramer + 2 K48polyUb-EPM2A", "inputs": [ { "node_id": "R-HSA-3781000", "reactome_id": "R-HSA-3781000", "label": "EPM2A:PPP1R3C:glycogen-GYG1:GYS1-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-113595", "reactome_id": "R-HSA-113595", "label": "Ub", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3781005", "reactome_id": "R-HSA-3781005", "label": "K48polyUb-PPP1R3C", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-3322059", "reactome_id": "R-HSA-3322059", "label": "glycogen-GYG1:GYS1-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-3781004", "reactome_id": "R-HSA-3781004", "label": "K48polyUb-EPM2A", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "ubiquitin-protein transferase activity of NHLRC1 [cytosol]", "activity_id": "3781003", "ec_numbers": [ "6.3.2.19" ], "accessions": [ "Q6VVB1" ] } ], "citations": [ { "id": "PMID:20538597", "title": "Genetic depletion of the malin E3 ubiquitin ligase in mice leads to lafora bodies and the accumulation of insoluble laforin", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20538597/" }, { "id": "PMID:15930137", "title": "Insights into Lafora disease: malin is an E3 ubiquitin ligase that ubiquitinates and promotes the degradation of laforin", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15930137/" }, { "id": "PMID:18070875", "title": "Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG)", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18070875/" } ], "x": 1530, "y": 1116.0384615384614, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-3781011", "label": "EPM2A dimer dephosphorylates phosphoglycogen-GYG2", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10685" ], "source_url": "https://reactome.org/content/detail/R-HSA-3781011", "summary": "EPM2A dimer dephosphorylates phosphoglycogen-GYG2 is a Reactome release 97 curated reaction event. The source record connects EPM2A:PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer, H2O to EPM2A:PPP1R3C:glycogen-GYG2:GYS2-a tetramer, Pi in cytosol and cross-references RHEA:10685.", "equation": "EPM2A:PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer + H2O → EPM2A:PPP1R3C:glycogen-GYG2:GYS2-a tetramer + Pi", "inputs": [ { "node_id": "R-HSA-3781017", "reactome_id": "R-HSA-3781017", "label": "EPM2A:PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-3780991", "reactome_id": "R-HSA-3780991", "label": "EPM2A:PPP1R3C:glycogen-GYG2:GYS2-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbohydrate phosphatase activity of EPM2A:PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer [cytosol]", "activity_id": "3781002", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25544560", "title": "Structural mechanism of laforin function in glycogen dephosphorylation and lafora disease", "journal": "Mol. Cell", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25544560/" }, { "id": "PMID:25538239", "title": "Dimeric quaternary structure of human laforin", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25538239/" }, { "id": "PMID:18040046", "title": "Laforin is a glycogen phosphatase, deficiency of which leads to elevated phosphorylation of glycogen in vivo", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/18040046/" } ], "x": 1808.6666666666667, "y": 876.6538461538462, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3781018", "label": "EPM2A dimer dephosphorylates phosphoglycogen-GYG1", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10685" ], "source_url": "https://reactome.org/content/detail/R-HSA-3781018", "summary": "EPM2A dimer dephosphorylates phosphoglycogen-GYG1 is a Reactome release 97 curated reaction event. The source record connects EPM2A:PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer, H2O to EPM2A:PPP1R3C:glycogen-GYG1:GYS1-a tetramer, Pi in cytosol and cross-references RHEA:10685.", "equation": "EPM2A:PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer + H2O → EPM2A:PPP1R3C:glycogen-GYG1:GYS1-a tetramer + Pi", "inputs": [ { "node_id": "R-HSA-3780999", "reactome_id": "R-HSA-3780999", "label": "EPM2A:PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-3781000", "reactome_id": "R-HSA-3781000", "label": "EPM2A:PPP1R3C:glycogen-GYG1:GYS1-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbohydrate phosphatase activity of EPM2A:PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer [cytosol]", "activity_id": "3781013", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25544560", "title": "Structural mechanism of laforin function in glycogen dephosphorylation and lafora disease", "journal": "Mol. Cell", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25544560/" }, { "id": "PMID:25538239", "title": "Dimeric quaternary structure of human laforin", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25538239/" }, { "id": "PMID:18040046", "title": "Laforin is a glycogen phosphatase, deficiency of which leads to elevated phosphorylation of glycogen in vivo", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/18040046/" } ], "x": 1251.3333333333333, "y": 906.5769230769231, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3781021", "label": "EPM2A dimer binds PPP1R3C:phosphoglycogen-GYG2 complex", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3781021", "summary": "EPM2A dimer binds PPP1R3C:phosphoglycogen-GYG2 complex is a Reactome release 97 curated reaction event. The source record connects EPM2A dimer, PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer to EPM2A:PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer in cytosol.", "equation": "EPM2A dimer + PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer → EPM2A:PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer", "inputs": [ { "node_id": "R-HSA-6805697", "reactome_id": "R-HSA-6805697", "label": "EPM2A dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-3777094", "reactome_id": "R-HSA-3777094", "label": "PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3781017", "reactome_id": "R-HSA-3781017", "label": "EPM2A:PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein-macromolecule adaptor activity of PPP1R3C:phosphoglycogen-GYG2:GYS2-a tetramer [cytosol]", "activity_id": "9931457", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18070875", "title": "Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG)", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18070875/" } ], "x": 1530, "y": 1056.1923076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3781023", "label": "PPP1R3C binds to glycogen:GYG1:GYS1", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3781023", "summary": "PPP1R3C binds to glycogen:GYG1:GYS1 is a Reactome release 97 curated reaction event. The source record connects PPP1R3C, glycogen-GYG1:GYS1-a tetramer to PPP1R3C:glycogen-GYG1:GYS1-a tetramer in cytosol.", "equation": "PPP1R3C + glycogen-GYG1:GYS1-a tetramer → PPP1R3C:glycogen-GYG1:GYS1-a tetramer", "inputs": [ { "node_id": "R-HSA-3777102", "reactome_id": "R-HSA-3777102", "label": "PPP1R3C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-3322059", "reactome_id": "R-HSA-3322059", "label": "glycogen-GYG1:GYS1-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3777096", "reactome_id": "R-HSA-3777096", "label": "PPP1R3C:glycogen-GYG1:GYS1-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein-macromolecule adaptor activity of PPP1R3C [cytosol]", "activity_id": "9931455", "ec_numbers": null, "accessions": [ "Q9UQK1" ] } ], "citations": [ { "id": "PMID:18070875", "title": "Malin decreases glycogen accumulation by promoting the degradation of protein targeting to glycogen (PTG)", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18070875/" } ], "x": 1808.6666666666667, "y": 1056.1923076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-3781024", "label": "GYS1 catalyzes the incorporation of phosphoglucose into glycogen-GYG1", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-3781024", "summary": "GYS1 catalyzes the incorporation of phosphoglucose into glycogen-GYG1 is a Reactome release 97 curated reaction event. The source record connects PPP1R3C:glycogen-GYG1:GYS1-a tetramer, UDP-Glc to PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer, UMP, H+ in cytosol.", "equation": "PPP1R3C:glycogen-GYG1:GYS1-a tetramer + UDP-Glc → PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer + UMP + H+", "inputs": [ { "node_id": "R-HSA-3777096", "reactome_id": "R-HSA-3777096", "label": "PPP1R3C:glycogen-GYG1:GYS1-a tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3777093", "reactome_id": "R-HSA-3777093", "label": "PPP1R3C:phosphoglycogen-GYG1:GYS1-a tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-109402", "reactome_id": "R-ALL-109402", "label": "UMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57865", "formula": "C9H11N2O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycogen synthase activity, transferring glucose-1-phosphate of PPP1R3C:glycogen-GYG1:GYS1-a tetramer [cytosol]", "activity_id": "3781012", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25416783", "title": "Glycogen phosphomonoester distribution in mouse models of the progressive myoclonic epilepsy, Lafora disease", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25416783/" }, { "id": "PMID:26216881", "title": "Muscle Glycogen Remodeling and Glycogen Phosphate Metabolism following Exhaustive Exercise of Wild Type and Laforin Knockout Mice", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26216881/" }, { "id": "PMID:23663739", "title": "Hyperphosphorylation of glucosyl C6 carbons and altered structure of glycogen in the neurodegenerative epilepsy Lafora disease", "journal": "Cell Metab.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23663739/" }, { "id": "PMID:21356517", "title": "Phosphate incorporation during glycogen synthesis and Lafora disease", "journal": "Cell Metab.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21356517/" } ], "x": 1251.3333333333333, "y": 966.4230769230769, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389540", "label": "isocitrate + NADP+ =\u003e 2-oxoglutarate + CO2 + NADPH + H+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19630" ], "source_url": "https://reactome.org/content/detail/R-HSA-389540", "summary": "isocitrate + NADP+ =\u003e 2-oxoglutarate + CO2 + NADPH + H+ is a Reactome release 97 curated reaction event. The source record connects ISCIT, NADP+ to 2OG, CO2, NADPH in cytosol and cross-references RHEA:19630.", "equation": "ISCIT + NADP+ → 2OG + CO2 + NADPH + H+", "inputs": [ { "node_id": "R-ALL-389545", "reactome_id": "R-ALL-389545", "label": "ISCIT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15562", "formula": "C6H5O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "isocitrate dehydrogenase (NADP+) activity of IDH1 dimer [cytosol]", "activity_id": "389538", "ec_numbers": [ "1.1.1.42" ], "accessions": [] } ], "citations": [ { "id": "PMID:10521434", "title": "The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10521434/" }, { "id": "PMID:15173171", "title": "Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15173171/" }, { "id": "PMID:11083877", "title": "Identification of the human mitochondrial oxodicarboxylate carrier. Bacterial expression, reconstitution, functional characterization, tissue distribution, and chromosomal location", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11083877/" } ], "x": 4730, "y": 3363.0416666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389550", "label": "isocitrate + NADP+ =\u003e 2-oxoglutarate + CO2 + NADPH + H+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:19630" ], "source_url": "https://reactome.org/content/detail/R-HSA-389550", "summary": "isocitrate + NADP+ =\u003e 2-oxoglutarate + CO2 + NADPH + H+ is a Reactome release 97 curated reaction event. The source record connects ISCIT, NADP+ to 2OG, CO2, NADPH in peroxisomal matrix and cross-references RHEA:19630.", "equation": "ISCIT + NADP+ → 2OG + CO2 + NADPH + H+", "inputs": [ { "node_id": "R-ALL-389548", "reactome_id": "R-ALL-389548", "label": "ISCIT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15562", "formula": "C6H5O7", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389556", "reactome_id": "R-ALL-389556", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-389537", "reactome_id": "R-ALL-389537", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389536", "reactome_id": "R-ALL-389536", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113602", "reactome_id": "R-ALL-113602", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "isocitrate dehydrogenase (NADP+) activity of IDH1 dimer [peroxisomal matrix]", "activity_id": "389544", "ec_numbers": [ "1.1.1.42" ], "accessions": [] } ], "citations": [ { "id": "PMID:15173171", "title": "Structures of human cytosolic NADP-dependent isocitrate dehydrogenase reveal a novel self-regulatory mechanism of activity", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15173171/" }, { "id": "PMID:10521434", "title": "The human PICD gene encodes a cytoplasmic and peroxisomal NADP(+)-dependent isocitrate dehydrogenase", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10521434/" } ], "x": 5008.666666666667, "y": 3363.0416666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389580", "label": "formyl-CoA + H2O =\u003e formate + CoASH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:19742" ], "source_url": "https://reactome.org/content/detail/R-HSA-389580", "summary": "formyl-CoA + H2O =\u003e formate + CoASH is a Reactome release 97 curated reaction event. The source record connects FOR-CoA, H2O to CoA-SH, HCOOH in peroxisomal matrix and cross-references RHEA:19742.", "equation": "FOR-CoA + H2O → CoA-SH + HCOOH", "inputs": [ { "node_id": "R-ALL-389578", "reactome_id": "R-ALL-389578", "label": "FOR-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57376", "formula": "C22H32N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389585", "reactome_id": "R-ALL-389585", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9166898", "title": "Production of formyl-CoA during peroxisomal alpha-oxidation of 3-methyl-branched fatty acids", "journal": "FEBS Lett", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9166898/" } ], "x": 868.6666666666667, "y": 1691.421052631579, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389609", "label": "ALDH3A2-2 oxidizes pristanal to pristanate", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:44017" ], "source_url": "https://reactome.org/content/detail/R-HSA-389609", "summary": "ALDH3A2-2 oxidizes pristanal to pristanate is a Reactome release 97 curated reaction event. The source record connects NAD+, Pristanal, H2O to pristanate, NADH, H+ in peroxisomal matrix and cross-references RHEA:44017.", "equation": "NAD+ + Pristanal + H2O → pristanate + NADH + 2 H+", "inputs": [ { "node_id": "R-ALL-192307", "reactome_id": "R-ALL-192307", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389636", "reactome_id": "R-ALL-389636", "label": "Pristanal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:49189", "formula": "C19H38O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6811612", "reactome_id": "R-ALL-6811612", "label": "pristanate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77268", "formula": "C19H37O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192305", "reactome_id": "R-ALL-192305", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of ALDH3A2-2 dimer [peroxisomal membrane]", "activity_id": "6811622", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:17510064", "title": "Dual subcellular localization in the endoplasmic reticulum and peroxisomes and a vital role in protecting against oxidative stress of fatty aldehyde dehydrogenase are achieved by alternative splicing", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17510064/" }, { "id": "PMID:11341778", "title": "Identification of pristanal dehydrogenase activity in peroxisomes: conclusive evidence that the complete phytanic acid alpha-oxidation pathway is localized in peroxisomes", "journal": "Biochem Biophys Res Commun", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11341778/" }, { "id": "PMID:19965611", "title": "Monitoring of fatty aldehyde dehydrogenase by formation of pyrenedecanoic acid from pyrenedecanal", "journal": "J. Lipid Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19965611/" }, { "id": "PMID:9133646", "title": "Human liver fatty aldehyde dehydrogenase: microsomal localization, purification, and biochemical characterization", "journal": "Biochim. Biophys. Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9133646/" }, { "id": "PMID:11306053", "title": "Fatty aldehyde dehydrogenase: genomic structure, expression and mutation analysis in Sjögren-Larsson syndrome", "journal": "Chem. Biol. Interact.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11306053/" } ], "x": 311.33333333333337, "y": 1537.5263157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389611", "label": "2-hydroxyphytanoyl-CoA =\u003e pristanal + formyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:25356" ], "source_url": "https://reactome.org/content/detail/R-HSA-389611", "summary": "2-hydroxyphytanoyl-CoA =\u003e pristanal + formyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3S2HPhy-CoA to Pristanal, FOR-CoA in peroxisomal matrix and cross-references RHEA:25356.", "equation": "3S2HPhy-CoA → Pristanal + FOR-CoA", "inputs": [ { "node_id": "R-ALL-389594", "reactome_id": "R-ALL-389594", "label": "3S2HPhy-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57334", "formula": "C41H70N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389636", "reactome_id": "R-ALL-389636", "label": "Pristanal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:49189", "formula": "C19H38O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389578", "reactome_id": "R-ALL-389578", "label": "FOR-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57376", "formula": "C22H32N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carbon-carbon lyase activity of HACL1 tetramer [peroxisomal matrix]", "activity_id": "389618", "ec_numbers": [ "4.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10468558", "title": "Purification, molecular cloning, and expression of 2-hydroxyphytanoyl-CoA lyase, a peroxisomal thiamine pyrophosphate-dependent enzyme that catalyzes the carbon-carbon bond cleavage during alpha-oxidation of 3-methyl-branched fatty acids", "journal": "Proc Natl Acad Sci U S A", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10468558/" }, { "id": "PMID:9166898", "title": "Production of formyl-CoA during peroxisomal alpha-oxidation of 3-methyl-branched fatty acids", "journal": "FEBS Lett", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9166898/" } ], "x": 311.33333333333337, "y": 1609.9473684210527, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389622", "label": "phytanate + CoA-SH + ATP =\u003e phytanoyl-CoA + AMP + pyrophosphate", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:21381" ], "source_url": "https://reactome.org/content/detail/R-HSA-389622", "summary": "phytanate + CoA-SH + ATP =\u003e phytanoyl-CoA + AMP + pyrophosphate is a Reactome release 97 curated reaction event. The source record connects ATP, CoA-SH, Phytanate to AMP, Phytanoyl-CoA, PPi in peroxisomal matrix and cross-references RHEA:21381.", "equation": "ATP + CoA-SH + Phytanate → AMP + Phytanoyl-CoA + PPi", "inputs": [ { "node_id": "R-ALL-389573", "reactome_id": "R-ALL-389573", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389596", "reactome_id": "R-ALL-389596", "label": "Phytanate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37257", "formula": "C20H39O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389620", "reactome_id": "R-ALL-389620", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389615", "reactome_id": "R-ALL-389615", "label": "Phytanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57391", "formula": "C41H70N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389593", "reactome_id": "R-ALL-389593", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phytanate-CoA ligase activity of SLC27A2 [peroxisomal membrane]", "activity_id": "389629", "ec_numbers": [ "6.2.1.24" ], "accessions": [ "O14975" ] } ], "citations": [ { "id": "PMID:10198260", "title": "Human very-long-chain acyl-CoA synthetase: cloning, topography, and relevance to branched-chain fatty acid metabolism", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10198260/" } ], "x": 590, "y": 1537.5263157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389632", "label": "pristanate + CoA-SH + ATP =\u003e pristanoyl-CoA + AMP + pyrophosphate", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:47265" ], "source_url": "https://reactome.org/content/detail/R-HSA-389632", "summary": "pristanate + CoA-SH + ATP =\u003e pristanoyl-CoA + AMP + pyrophosphate is a Reactome release 97 curated reaction event. The source record connects ATP, CoA-SH, pristanate to AMP, (2R) Pristanoyl-CoA, PPi in peroxisomal matrix and cross-references RHEA:47265.", "equation": "ATP + CoA-SH + pristanate → AMP + (2R) Pristanoyl-CoA + PPi", "inputs": [ { "node_id": "R-ALL-389573", "reactome_id": "R-ALL-389573", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-6811612", "reactome_id": "R-ALL-6811612", "label": "pristanate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77268", "formula": "C19H37O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389620", "reactome_id": "R-ALL-389620", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389569", "reactome_id": "R-ALL-389569", "label": "(2R) Pristanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77250", "formula": "C40H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389593", "reactome_id": "R-ALL-389593", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pristanate-CoA ligase activity of SLC27A2 [peroxisomal membrane]", "activity_id": "389637", "ec_numbers": [ "6.2.1" ], "accessions": [ "O14975" ] } ], "citations": [ { "id": "PMID:10198260", "title": "Human very-long-chain acyl-CoA synthetase: cloning, topography, and relevance to branched-chain fatty acid metabolism", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10198260/" }, { "id": "PMID:9662422", "title": "Involvement of microsomal fatty aldehyde dehydrogenase in the alpha-oxidation of phytanic acid", "journal": "FEBS Lett", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9662422/" } ], "x": 868.6666666666667, "y": 1537.5263157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389639", "label": "phytanoyl-CoA + 2-oxoglutarate + O2 =\u003e 2-hydroxyphytanoyl-CoA + succinate + CO2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:16066" ], "source_url": "https://reactome.org/content/detail/R-HSA-389639", "summary": "phytanoyl-CoA + 2-oxoglutarate + O2 =\u003e 2-hydroxyphytanoyl-CoA + succinate + CO2 is a Reactome release 97 curated reaction event. The source record connects Phytanoyl-CoA, 2OG, O2 to 3S2HPhy-CoA, SUCCA, CO2 in peroxisomal matrix and cross-references RHEA:16066.", "equation": "Phytanoyl-CoA + 2OG + O2 → 3S2HPhy-CoA + SUCCA + CO2", "inputs": [ { "node_id": "R-ALL-389615", "reactome_id": "R-ALL-389615", "label": "Phytanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57391", "formula": "C41H70N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389537", "reactome_id": "R-ALL-389537", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389594", "reactome_id": "R-ALL-389594", "label": "3S2HPhy-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57334", "formula": "C41H70N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389583", "reactome_id": "R-ALL-389583", "label": "SUCCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30031", "formula": "C4H4O4", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389536", "reactome_id": "R-ALL-389536", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phytanoyl-CoA dioxygenase activity of PHYH:Fe++ [peroxisomal matrix]", "activity_id": "389595", "ec_numbers": [ "1.14.11.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:16186124", "title": "Structure of human phytanoyl-CoA 2-hydroxylase identifies molecular mechanisms of Refsum disease", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16186124/" }, { "id": "PMID:11555634", "title": "Structure-function analysis of phytanoyl-CoA 2-hydroxylase mutations causing Refsum's disease", "journal": "Hum Mol Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11555634/" } ], "x": 311.33333333333337, "y": 1540.5438596491229, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389652", "label": "PMP34-mediated exchange of cytosolic ATP for peroxisomal AMP", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal membrane", "compartment_ids": [ "GO:0005778" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-389652", "summary": "PMP34-mediated exchange of cytosolic ATP for peroxisomal AMP is a Reactome release 97 curated reaction event. The source record connects AMP, ATP to AMP, ATP in peroxisomal membrane.", "equation": "AMP + ATP → AMP + ATP", "inputs": [ { "node_id": "R-ALL-389620", "reactome_id": "R-ALL-389620", "label": "AMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389573", "reactome_id": "R-ALL-389573", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "adenine nucleotide transmembrane transporter activity of SLC25A17 [peroxisomal membrane]", "activity_id": "389649", "ec_numbers": null, "accessions": [ "O43808" ] } ], "citations": [ { "id": "PMID:16756494", "title": "Biochemistry of mammalian peroxisomes revisited", "journal": "Annu Rev Biochem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16756494/" }, { "id": "PMID:9874197", "title": "Identification and characterization of human PMP34, a protein closely related to the peroxisomal integral membrane protein PMP47 of Candida boidinii", "journal": "Eur J Biochem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9874197/" }, { "id": "PMID:12445829", "title": "Identification of human PMP34 as a peroxisomal ATP transporter", "journal": "Biochem Biophys Res Commun", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12445829/" } ], "x": 311.33333333333337, "y": 1715.561403508772, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389684", "label": "glyoxylate + alanine =\u003e glycine + pyruvate [peroxisome]", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:24249" ], "source_url": "https://reactome.org/content/detail/R-HSA-389684", "summary": "glyoxylate + alanine =\u003e glycine + pyruvate [peroxisome] is a Reactome release 97 curated reaction event. The source record connects glyoxylate, L-Ala to Gly, PYR in peroxisomal matrix and cross-references RHEA:24249.", "equation": "glyoxylate + L-Ala → Gly + PYR", "inputs": [ { "node_id": "R-ALL-389678", "reactome_id": "R-ALL-389678", "label": "glyoxylate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389664", "reactome_id": "R-ALL-389664", "label": "L-Ala", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193446", "reactome_id": "R-ALL-193446", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389680", "reactome_id": "R-ALL-389680", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-alanine:glyoxylate transaminase activity of AGXT dimer [peroxisomal matrix]", "activity_id": "389659", "ec_numbers": [ "2.6.1.44" ], "accessions": [] } ], "citations": [ { "id": "PMID:15802217", "title": "Overexpression of human alanine:glyoxylate aminotransferase in Escherichia coli: renaturation from guanidine-HCl and affinity for pyridoxal phosphate co-factor", "journal": "Protein Expr Purif", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15802217/" }, { "id": "PMID:3416563", "title": "Further studies on the activity and subcellular distribution of alanine:glyoxylate aminotransferase in the livers of patients with primary hyperoxaluria type 1", "journal": "Clin Sci (Lond)", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3416563/" }, { "id": "PMID:1703535", "title": "Identification of mutations associated with peroxisome-to-mitochondrion mistargeting of alanine/glyoxylate aminotransferase in primary hyperoxaluria type 1", "journal": "J Cell Biol", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/1703535/" }, { "id": "PMID:2363689", "title": "Human peroxisomal L-alanine: glyoxylate aminotransferase. Evolutionary loss of a mitochondrial targeting signal by point mutation of the initiation codon", "journal": "Biochem J", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2363689/" }, { "id": "PMID:12899834", "title": "Crystal structure of alanine:glyoxylate aminotransferase and the relationship between genotype and enzymatic phenotype in primary hyperoxaluria type 1", "journal": "J Mol Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12899834/" } ], "x": 590, "y": 3204.2380952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389821", "label": "glycine + O2 =\u003e glyoxylate + H2O2 + NH4+", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:11533" ], "source_url": "https://reactome.org/content/detail/R-HSA-389821", "summary": "glycine + O2 =\u003e glyoxylate + H2O2 + NH4+ is a Reactome release 97 curated reaction event. The source record connects Gly, H2O, O2 to glyoxylate, H2O2, NH4+ in peroxisomal matrix and cross-references RHEA:11533.", "equation": "Gly + H2O + O2 → glyoxylate + H2O2 + NH4+", "inputs": [ { "node_id": "R-ALL-193446", "reactome_id": "R-ALL-193446", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389678", "reactome_id": "R-ALL-389678", "label": "glyoxylate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389843", "reactome_id": "R-ALL-389843", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "D-amino-acid oxidase activity of DAO dimer [peroxisomal matrix]", "activity_id": "389825", "ec_numbers": [ "1.4.3.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:17088322", "title": "Crystal structure of human D-amino acid oxidase: context-dependent variability of the backbone conformation of the VAAGL hydrophobic stretch located at the si-face of the flavin ring", "journal": "Protein Sci", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17088322/" }, { "id": "PMID:16616139", "title": "Characterization of human D-amino acid oxidase", "journal": "FEBS Lett", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16616139/" } ], "x": 311.33333333333337, "y": 3138.714285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389826", "label": "glyoxylate + NADPH + H+ =\u003e glycolate + NADP+", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:10994" ], "source_url": "https://reactome.org/content/detail/R-HSA-389826", "summary": "glyoxylate + NADPH + H+ =\u003e glycolate + NADP+ is a Reactome release 97 curated reaction event. The source record connects glyoxylate, NADPH, H+ to NADP+, glycolate in peroxisomal matrix and cross-references RHEA:10994.", "equation": "glyoxylate + NADPH + H+ → NADP+ + glycolate", "inputs": [ { "node_id": "R-ALL-389678", "reactome_id": "R-ALL-389678", "label": "glyoxylate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113602", "reactome_id": "R-ALL-113602", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-389556", "reactome_id": "R-ALL-389556", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389822", "reactome_id": "R-ALL-389822", "label": "glycolate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29805", "formula": "C2H3O3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glyoxylate reductase (NADPH) activity of GRHPR [peroxisomal matrix]", "activity_id": "389805", "ec_numbers": [ "1.1.1.79" ], "accessions": [ "Q9UBQ7" ] } ], "citations": [ { "id": "PMID:10524214", "title": "Identification and expression of a cDNA for human hydroxypyruvate/glyoxylate reductase", "journal": "Biochim Biophys Acta", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10524214/" }, { "id": "PMID:10484776", "title": "The gene encoding hydroxypyruvate reductase (GRHPR) is mutated in patients with primary hyperoxaluria type II", "journal": "Hum Mol Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10484776/" } ], "x": 311.33333333333337, "y": 3171.476190476191, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389842", "label": "HAO1 tetramer oxidizes glycolate to glyoxylate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:25312" ], "source_url": "https://reactome.org/content/detail/R-HSA-389842", "summary": "HAO1 tetramer oxidizes glycolate to glyoxylate is a Reactome release 97 curated reaction event. The source record connects glycolate, O2 to glyoxylate, H2O2 in peroxisomal matrix and cross-references RHEA:25312.", "equation": "glycolate + O2 → glyoxylate + H2O2", "inputs": [ { "node_id": "R-ALL-389822", "reactome_id": "R-ALL-389822", "label": "glycolate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29805", "formula": "C2H3O3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389678", "reactome_id": "R-ALL-389678", "label": "glyoxylate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(S)-2-hydroxy-acid oxidase activity of HAO1 tetramer [peroxisomal matrix]", "activity_id": "9010884", "ec_numbers": [ "1.1.3.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:10978532", "title": "Identification and expression of a cDNA for human glycolate oxidase", "journal": "Biochim Biophys Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10978532/" }, { "id": "PMID:18215067", "title": "Active site and loop 4 movements within human glycolate oxidase: implications for substrate specificity and drug design", "journal": "Biochemistry", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18215067/" }, { "id": "PMID:17669354", "title": "Purification and characterization of recombinant human liver glycolate oxidase", "journal": "Arch Biochem Biophys", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17669354/" }, { "id": "PMID:10777549", "title": "Identification and characterization of HAOX1, HAOX2, and HAOX3, three human peroxisomal 2-hydroxy acid oxidases", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777549/" } ], "x": 868.6666666666667, "y": 3204.2380952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389862", "label": "Conversion of glyoxylate to oxalate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:14838" ], "source_url": "https://reactome.org/content/detail/R-HSA-389862", "summary": "Conversion of glyoxylate to oxalate is a Reactome release 97 curated reaction event. The source record connects glyoxylate, H2O, O2 to OX, H2O2 in peroxisomal matrix and cross-references RHEA:14838.", "equation": "glyoxylate + H2O + O2 → OX + H2O2", "inputs": [ { "node_id": "R-ALL-389678", "reactome_id": "R-ALL-389678", "label": "glyoxylate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389819", "reactome_id": "R-ALL-389819", "label": "OX", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30623", "formula": "C2O4", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glyoxylate oxidase activity of HAO1 tetramer [peroxisomal matrix]", "activity_id": "389855", "ec_numbers": [ "1.2.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:10978532", "title": "Identification and expression of a cDNA for human glycolate oxidase", "journal": "Biochim Biophys Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10978532/" }, { "id": "PMID:18215067", "title": "Active site and loop 4 movements within human glycolate oxidase: implications for substrate specificity and drug design", "journal": "Biochemistry", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18215067/" }, { "id": "PMID:17669354", "title": "Purification and characterization of recombinant human liver glycolate oxidase", "journal": "Arch Biochem Biophys", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17669354/" }, { "id": "PMID:10777549", "title": "Identification and characterization of HAOX1, HAOX2, and HAOX3, three human peroxisomal 2-hydroxy acid oxidases", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777549/" } ], "x": 590, "y": 3171.476190476191, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389889", "label": "ACOX2:FAD, ACOXL:FAD oxidise (2S)-pristanoyl-CoA to trans-2,3-dehydropristanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:40460" ], "source_url": "https://reactome.org/content/detail/R-HSA-389889", "summary": "ACOX2:FAD, ACOXL:FAD oxidise (2S)-pristanoyl-CoA to trans-2,3-dehydropristanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects (2S) Pristanoyl-CoA, O2 to trans-2,3-dehydropristanoyl-CoA, H2O2 in peroxisomal matrix and cross-references RHEA:40460.", "equation": "(2S) Pristanoyl-CoA + O2 → trans-2,3-dehydropristanoyl-CoA + H2O2", "inputs": [ { "node_id": "R-ALL-389893", "reactome_id": "R-ALL-389893", "label": "(2S) Pristanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77099", "formula": "C40H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389894", "reactome_id": "R-ALL-389894", "label": "trans-2,3-dehydropristanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77293", "formula": "C40H66N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pristanoyl-CoA oxidase activity of ACOX2:FAD, ACOXL:FAD [peroxisomal matrix]", "activity_id": "389900", "ec_numbers": [ "1.3.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:8387517", "title": "The CoA esters of 2-methyl-branched chain fatty acids and of the bile acid intermediates di- and trihydroxycoprostanic acids are oxidized by one single peroxisomal branched chain acyl-CoA oxidase in human liver and kidney", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8387517/" }, { "id": "PMID:8943006", "title": "Molecular characterization of the human peroxisomal branched-chain acyl-CoA oxidase: cDNA cloning, chromosomal assignment, tissue distribution, and evidence for the absence of the protein in Zellweger syndrome", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8943006/" } ], "x": 868.6666666666667, "y": 1576.7543859649122, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389891", "label": "(2S)-pristanoyl-CoA + O2 =\u003e trans-2,3-dehydropristanoyl-CoA + H2O2 (ACOX3)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:40460" ], "source_url": "https://reactome.org/content/detail/R-HSA-389891", "summary": "(2S)-pristanoyl-CoA + O2 =\u003e trans-2,3-dehydropristanoyl-CoA + H2O2 (ACOX3) is a Reactome release 97 curated reaction event. The source record connects (2S) Pristanoyl-CoA, O2 to trans-2,3-dehydropristanoyl-CoA, H2O2 in peroxisomal matrix and cross-references RHEA:40460.", "equation": "(2S) Pristanoyl-CoA + O2 → trans-2,3-dehydropristanoyl-CoA + H2O2", "inputs": [ { "node_id": "R-ALL-389893", "reactome_id": "R-ALL-389893", "label": "(2S) Pristanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77099", "formula": "C40H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389894", "reactome_id": "R-ALL-389894", "label": "trans-2,3-dehydropristanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77293", "formula": "C40H66N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pristanoyl-CoA oxidase activity of ACOX3:FAD [peroxisomal matrix]", "activity_id": "389903", "ec_numbers": [ "1.3.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:15599942", "title": "Peroxisomal branched chain fatty acid beta-oxidation pathway is upregulated in prostate cancer", "journal": "Prostate", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15599942/" }, { "id": "PMID:9271077", "title": "Evidence for the existence of a pristanoyl-CoA oxidase gene in man", "journal": "Biochem J", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9271077/" } ], "x": 311.33333333333337, "y": 1579.7719298245613, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389897", "label": "Isomerization of (2R)-pristanoyl-CoA to (2S)-pristanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:40448" ], "source_url": "https://reactome.org/content/detail/R-HSA-389897", "summary": "Isomerization of (2R)-pristanoyl-CoA to (2S)-pristanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects (2R) Pristanoyl-CoA to (2S) Pristanoyl-CoA in peroxisomal matrix and cross-references RHEA:40448.", "equation": "(2R) Pristanoyl-CoA → (2S) Pristanoyl-CoA", "inputs": [ { "node_id": "R-ALL-389569", "reactome_id": "R-ALL-389569", "label": "(2R) Pristanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77250", "formula": "C40H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389893", "reactome_id": "R-ALL-389893", "label": "(2S) Pristanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77099", "formula": "C40H68N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "racemase and epimerase activity of AMACR [peroxisomal matrix]", "activity_id": "191973", "ec_numbers": [ "5.1" ], "accessions": [ "Q9UHK6" ] } ], "citations": [ { "id": "PMID:7649182", "title": "Purification and characterization of an alpha-methylacyl-CoA racemase from human liver", "journal": "Eur J Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7649182/" }, { "id": "PMID:11060344", "title": "Mitochondrial and peroxisomal targeting of 2-methylacyl-CoA racemase in humans", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11060344/" }, { "id": "PMID:11060359", "title": "Subcellular localization and physiological role of alpha-methylacyl-CoA racemase", "journal": "J Lipid Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11060359/" } ], "x": 311.33333333333337, "y": 1691.421052631579, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389986", "label": "trans-2,3-dehydropristanoyl-CoA + H2O =\u003e 3-hydroxypristanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-389986", "summary": "trans-2,3-dehydropristanoyl-CoA + H2O =\u003e 3-hydroxypristanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects trans-2,3-dehydropristanoyl-CoA, H2O to 3-hydroxypristanoyl-CoA in peroxisomal matrix.", "equation": "trans-2,3-dehydropristanoyl-CoA + H2O → 3-hydroxypristanoyl-CoA", "inputs": [ { "node_id": "R-ALL-389894", "reactome_id": "R-ALL-389894", "label": "trans-2,3-dehydropristanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77293", "formula": "C40H66N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-389982", "reactome_id": "R-ALL-389982", "label": "3-hydroxypristanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63914", "formula": "C40H72N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-hydroxyacyl-CoA dehydratase activity of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "389989", "ec_numbers": [ "4.2.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:16385454", "title": "Mutational spectrum of D-bifunctional protein deficiency and structure-based genotype-phenotype analysis", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16385454/" }, { "id": "PMID:8902629", "title": "Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase", "journal": "J Biochem (Tokyo)", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8902629/" }, { "id": "PMID:9089413", "title": "Structure of D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein", "journal": "J Biochem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9089413/" }, { "id": "PMID:15060085", "title": "Identification of the peroxisomal beta-oxidation enzymes involved in the degradation of long-chain dicarboxylic acids", "journal": "J. Lipid Res.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15060085/" } ], "x": 311.33333333333337, "y": 1724.6140350877192, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-389995", "label": "3-hydroxypristanoyl-CoA + NAD+ =\u003e 3-ketoxypristanoyl-CoA + NADH + H+", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-389995", "summary": "3-hydroxypristanoyl-CoA + NAD+ =\u003e 3-ketoxypristanoyl-CoA + NADH + H+ is a Reactome release 97 curated reaction event. The source record connects 3-hydroxypristanoyl-CoA, NAD+ to 3-ketopristanoyl-CoA, NADH, H+ in peroxisomal matrix.", "equation": "3-hydroxypristanoyl-CoA + NAD+ → 3-ketopristanoyl-CoA + NADH + H+", "inputs": [ { "node_id": "R-ALL-389982", "reactome_id": "R-ALL-389982", "label": "3-hydroxypristanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63914", "formula": "C40H72N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192307", "reactome_id": "R-ALL-192307", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-389996", "reactome_id": "R-ALL-389996", "label": "3-ketopristanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57291", "formula": "C40H66N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192305", "reactome_id": "R-ALL-192305", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "193365", "ec_numbers": [ "1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16385454", "title": "Mutational spectrum of D-bifunctional protein deficiency and structure-based genotype-phenotype analysis", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16385454/" }, { "id": "PMID:8902629", "title": "Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase", "journal": "J Biochem (Tokyo)", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8902629/" }, { "id": "PMID:9089413", "title": "Structure of D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein", "journal": "J Biochem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9089413/" } ], "x": 868.6666666666667, "y": 1709.5263157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390224", "label": "3-ketopristanoyl-CoA + CoASH =\u003e 4,8,12-trimethyltridecanoyl-CoA + propionyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:10410" ], "source_url": "https://reactome.org/content/detail/R-HSA-390224", "summary": "3-ketopristanoyl-CoA + CoASH =\u003e 4,8,12-trimethyltridecanoyl-CoA + propionyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-ketopristanoyl-CoA, CoA-SH to 4,8,12-trimethyltridecanoyl-CoA, propionyl CoA in peroxisomal matrix and cross-references RHEA:10410.", "equation": "3-ketopristanoyl-CoA + CoA-SH → 4,8,12-trimethyltridecanoyl-CoA + propionyl CoA", "inputs": [ { "node_id": "R-ALL-389996", "reactome_id": "R-ALL-389996", "label": "3-ketopristanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57291", "formula": "C40H66N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-390002", "reactome_id": "R-ALL-390002", "label": "4,8,12-trimethyltridecanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57351", "formula": "C37H62N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192314", "reactome_id": "R-ALL-192314", "label": "propionyl CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57392", "formula": "C24H36N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "propanoyl-CoA C-acyltransferase activity of SCP2-1 [peroxisomal matrix]", "activity_id": "192310", "ec_numbers": [ "2.3.1.176" ], "accessions": [ "P22307" ] } ], "citations": [ { "id": "PMID:16685654", "title": "Mutations in the gene encoding peroxisomal sterol carrier protein X (SCPx) cause leukencephalopathy with dystonia and motor neuropathy", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16685654/" }, { "id": "PMID:9245689", "title": "Sterol carrier protein X (SCPx) is a peroxisomal branched-chain beta-ketothiolase specifically reacting with 3-oxo-pristanoyl-CoA: a new, unique role for SCPx in branched-chain fatty acid metabolism in peroxisomes", "journal": "Biochem Biophys Res Commun", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9245689/" } ], "x": 590, "y": 1579.7719298245613, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390250", "label": "3-ketohexacosanoyl-CoA + CoASH =\u003e tetracosanoyl-CoA + acetyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:78641" ], "source_url": "https://reactome.org/content/detail/R-HSA-390250", "summary": "3-ketohexacosanoyl-CoA + CoASH =\u003e tetracosanoyl-CoA + acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-ketohexacosanoyl-CoA, CoA-SH to tetracosanoyl-CoA, Ac-CoA in peroxisomal matrix and cross-references RHEA:78641.", "equation": "3-ketohexacosanoyl-CoA + CoA-SH → tetracosanoyl-CoA + Ac-CoA", "inputs": [ { "node_id": "R-ALL-390241", "reactome_id": "R-ALL-390241", "label": "3-ketohexacosanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:73980", "formula": "C47H80N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-390246", "reactome_id": "R-ALL-390246", "label": "tetracosanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65052", "formula": "C45H78N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-353123", "reactome_id": "R-ALL-353123", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acyltransferase activity of ACAA1 [peroxisomal matrix]", "activity_id": "390234", "ec_numbers": [ "2.3.1.16" ], "accessions": [ "P09110" ] } ], "citations": [ { "id": "PMID:1679347", "title": "Characterization of the gene encoding human peroxisomal 3-oxoacyl-CoA thiolase (ACAA). No large DNA rearrangement in a thiolase-deficient patient", "journal": "Biochim Biophys Acta", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1679347/" } ], "x": 868.6666666666667, "y": 1579.7719298245613, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390251", "label": "HSD17B4 dehydrogenates 3-hydroxyhexacosanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:78637" ], "source_url": "https://reactome.org/content/detail/R-HSA-390251", "summary": "HSD17B4 dehydrogenates 3-hydroxyhexacosanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-hydroxyhexacosanoyl-CoA, NAD+ to 3-ketohexacosanoyl-CoA, NADH, H+ in peroxisomal matrix and cross-references RHEA:78637.", "equation": "3-hydroxyhexacosanoyl-CoA + NAD+ → 3-ketohexacosanoyl-CoA + NADH + H+", "inputs": [ { "node_id": "R-ALL-390248", "reactome_id": "R-ALL-390248", "label": "3-hydroxyhexacosanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:76378", "formula": "C47H82N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192307", "reactome_id": "R-ALL-192307", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-390241", "reactome_id": "R-ALL-390241", "label": "3-ketohexacosanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73980", "formula": "C47H80N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192305", "reactome_id": "R-ALL-192305", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "193365", "ec_numbers": [ "1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16385454", "title": "Mutational spectrum of D-bifunctional protein deficiency and structure-based genotype-phenotype analysis", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16385454/" }, { "id": "PMID:8902629", "title": "Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase", "journal": "J Biochem (Tokyo)", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8902629/" }, { "id": "PMID:9089413", "title": "Structure of D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein", "journal": "J Biochem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9089413/" } ], "x": 590, "y": 1555.6315789473683, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390252", "label": "HSD17B4 hydrates trans-2,3-dehydrohexacosanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:39213" ], "source_url": "https://reactome.org/content/detail/R-HSA-390252", "summary": "HSD17B4 hydrates trans-2,3-dehydrohexacosanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects trans-2,3-dehydrohexacosanoyl-CoA, H2O to 3-hydroxyhexacosanoyl-CoA in peroxisomal matrix and cross-references RHEA:39213.", "equation": "trans-2,3-dehydrohexacosanoyl-CoA + H2O → 3-hydroxyhexacosanoyl-CoA", "inputs": [ { "node_id": "R-ALL-390249", "reactome_id": "R-ALL-390249", "label": "trans-2,3-dehydrohexacosanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:74281", "formula": "C47H80N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-390248", "reactome_id": "R-ALL-390248", "label": "3-hydroxyhexacosanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76378", "formula": "C47H82N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-hydroxyacyl-CoA dehydratase activity of HSD17B4 dimer [peroxisomal matrix]", "activity_id": "389989", "ec_numbers": [ "4.2.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:16385454", "title": "Mutational spectrum of D-bifunctional protein deficiency and structure-based genotype-phenotype analysis", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16385454/" }, { "id": "PMID:8902629", "title": "Purification and properties of human D-3-hydroxyacyl-CoA dehydratase: medium-chain enoyl-CoA hydratase is D-3-hydroxyacyl-CoA dehydratase", "journal": "J Biochem (Tokyo)", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8902629/" }, { "id": "PMID:9089413", "title": "Structure of D-3-hydroxyacyl-CoA dehydratase/D-3-hydroxyacyl-CoA dehydrogenase bifunctional protein", "journal": "J Biochem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9089413/" } ], "x": 590, "y": 1609.9473684210527, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390256", "label": "ACOX1 oxidizes C26:0 CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:78632" ], "source_url": "https://reactome.org/content/detail/R-HSA-390256", "summary": "ACOX1 oxidizes C26:0 CoA is a Reactome release 97 curated reaction event. The source record connects C26:0 CoA, O2 to trans-2,3-dehydrohexacosanoyl-CoA, H2O2 in peroxisomal matrix and cross-references RHEA:78632.", "equation": "C26:0 CoA + O2 → trans-2,3-dehydrohexacosanoyl-CoA + H2O2", "inputs": [ { "node_id": "R-ALL-390260", "reactome_id": "R-ALL-390260", "label": "C26:0 CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64868", "formula": "C47H82N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-390249", "reactome_id": "R-ALL-390249", "label": "trans-2,3-dehydrohexacosanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:74281", "formula": "C47H80N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA oxidase activity of ACOX1 dimer [peroxisomal matrix]", "activity_id": "390231", "ec_numbers": [ "1.3.3.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:7876265", "title": "Overexpression and characterization of the human peroxisomal acyl-CoA oxidase in insect cells", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7876265/" }, { "id": "PMID:17458872", "title": "Clinical, biochemical, and mutational spectrum of peroxisomal acyl-coenzyme A oxidase deficiency", "journal": "Hum Mutat", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17458872/" } ], "x": 311.33333333333337, "y": 1582.7894736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390276", "label": "Conversion of 4,8,12-trimethyltridecanoyl-CoA to 4,8-dimethylnonanoyl-CoA", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-390276", "summary": "Conversion of 4,8,12-trimethyltridecanoyl-CoA to 4,8-dimethylnonanoyl-CoA is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 4,8,12-trimethyltridecanoyl-CoA to 4,8-dimethylnonanoyl-CoA in peroxisomal matrix.", "equation": "4,8,12-trimethyltridecanoyl-CoA → 4,8-dimethylnonanoyl-CoA", "inputs": [ { "node_id": "R-ALL-390002", "reactome_id": "R-ALL-390002", "label": "4,8,12-trimethyltridecanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57351", "formula": "C37H62N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-390271", "reactome_id": "R-ALL-390271", "label": "4,8-dimethylnonanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77061", "formula": "C32H52N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9469587", "title": "Phytanic acid and pristanic acid are oxidized by sequential peroxisomal and mitochondrial reactions in cultured fibroblasts", "journal": "J Lipid Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9469587/" } ], "x": 311.33333333333337, "y": 1760.8245614035088, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-390281", "label": "4,8-dimethylnonanoyl-CoA + carnitine =\u003e 4,8-dimethylnonanoylcarnitine + CoASH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:44861" ], "source_url": "https://reactome.org/content/detail/R-HSA-390281", "summary": "4,8-dimethylnonanoyl-CoA + carnitine =\u003e 4,8-dimethylnonanoylcarnitine + CoASH is a Reactome release 97 curated reaction event. The source record connects 4,8-dimethylnonanoyl-CoA, CAR to 4,8-dimethylnonanoylcarnitine, CoA-SH in peroxisomal matrix and cross-references RHEA:44861.", "equation": "4,8-dimethylnonanoyl-CoA + CAR → 4,8-dimethylnonanoylcarnitine + CoA-SH", "inputs": [ { "node_id": "R-ALL-390271", "reactome_id": "R-ALL-390271", "label": "4,8-dimethylnonanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77061", "formula": "C32H52N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-390294", "reactome_id": "R-ALL-390294", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-390290", "reactome_id": "R-ALL-390290", "label": "4,8-dimethylnonanoylcarnitine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84654", "formula": "C18H35NO4", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carnitine O-octanoyltransferase activity of CROT [peroxisomal matrix]", "activity_id": "390282", "ec_numbers": [ "2.3.1.137" ], "accessions": [ "Q9UKG9" ] } ], "citations": [ { "id": "PMID:10486279", "title": "Molecular cloning and expression of human carnitine octanoyltransferase: evidence for its role in the peroxisomal beta-oxidation of branched-chain fatty acids", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10486279/" } ], "x": 590, "y": 1582.7894736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390284", "label": "propionyl-CoA + carnitine =\u003e propionylcarnitine + CoASH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:44977" ], "source_url": "https://reactome.org/content/detail/R-HSA-390284", "summary": "propionyl-CoA + carnitine =\u003e propionylcarnitine + CoASH is a Reactome release 97 curated reaction event. The source record connects propionyl CoA, CAR to Propionylcarnitine, CoA-SH in peroxisomal matrix and cross-references RHEA:44977.", "equation": "propionyl CoA + CAR → Propionylcarnitine + CoA-SH", "inputs": [ { "node_id": "R-ALL-192314", "reactome_id": "R-ALL-192314", "label": "propionyl CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57392", "formula": "C24H36N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-390294", "reactome_id": "R-ALL-390294", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-390274", "reactome_id": "R-ALL-390274", "label": "Propionylcarnitine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:53210", "formula": "C10H19NO4", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carnitine O-acetyltransferase activity of CRAT [peroxisomal matrix]", "activity_id": "390278", "ec_numbers": [ "2.3.1.7" ], "accessions": [ "P43155" ] } ], "citations": [ { "id": "PMID:2351134", "title": "Purification and properties of carnitine acetyltransferase from human liver", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2351134/" }, { "id": "PMID:12562770", "title": "Structure of human carnitine acetyltransferase. Molecular basis for fatty acyl transfer.", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12562770/" } ], "x": 868.6666666666667, "y": 1582.7894736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390291", "label": "acetyl-CoA + carnitine =\u003e acetylcarnitine + CoASH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:21137" ], "source_url": "https://reactome.org/content/detail/R-HSA-390291", "summary": "acetyl-CoA + carnitine =\u003e acetylcarnitine + CoASH is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, CAR to ACAR, CoA-SH in peroxisomal matrix and cross-references RHEA:21137.", "equation": "Ac-CoA + CAR → ACAR + CoA-SH", "inputs": [ { "node_id": "R-ALL-353123", "reactome_id": "R-ALL-353123", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-390294", "reactome_id": "R-ALL-390294", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-390287", "reactome_id": "R-ALL-390287", "label": "ACAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57589", "formula": "C9H17NO4", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carnitine O-acetyltransferase activity of CRAT [peroxisomal matrix]", "activity_id": "390278", "ec_numbers": [ "2.3.1.7" ], "accessions": [ "P43155" ] } ], "citations": [ { "id": "PMID:2351134", "title": "Purification and properties of carnitine acetyltransferase from human liver", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2351134/" }, { "id": "PMID:15099582", "title": "Structural and mutational characterization of L-carnitine binding to human carnitine acetyltransferase", "journal": "J Struct Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15099582/" }, { "id": "PMID:12562770", "title": "Structure of human carnitine acetyltransferase. Molecular basis for fatty acyl transfer.", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12562770/" } ], "x": 311.33333333333337, "y": 1585.8070175438597, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390302", "label": "Beta-oxidation of tetradecenoyl-CoA to octanoyl-CoA", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-390302", "summary": "Beta-oxidation of tetradecenoyl-CoA to octanoyl-CoA is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CoA-SH, H2O, NAD+ to Ac-CoA, H+, H2O2 in peroxisomal matrix.", "equation": "8 CoA-SH + 8 H2O + 8 NAD+ + 8 O2 + tetracosanoyl-CoA → 8 Ac-CoA + 8 H+ + 8 H2O2 + 8 NADH + Octanoyl-CoA", "inputs": [ { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-192307", "reactome_id": "R-ALL-192307", "label": "NAD+", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-390246", "reactome_id": "R-ALL-390246", "label": "tetracosanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65052", "formula": "C45H78N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-353123", "reactome_id": "R-ALL-353123", "label": "Ac-CoA", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192305", "reactome_id": "R-ALL-192305", "label": "NADH", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-390303", "reactome_id": "R-ALL-390303", "label": "Octanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57386", "formula": "C29H46N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16756494", "title": "Biochemistry of mammalian peroxisomes revisited", "journal": "Annu Rev Biochem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16756494/" } ], "x": 590, "y": 1727.6315789473683, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-390304", "label": "acetyl-CoA + H2O =\u003e acetate + CoASH + H+", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:20290" ], "source_url": "https://reactome.org/content/detail/R-HSA-390304", "summary": "acetyl-CoA + H2O =\u003e acetate + CoASH + H+ is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, H2O to CH3COO-, CoA-SH, H+ in peroxisomal matrix and cross-references RHEA:20290.", "equation": "Ac-CoA + H2O → CH3COO- + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-353123", "reactome_id": "R-ALL-353123", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-390305", "reactome_id": "R-ALL-390305", "label": "CH3COO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acetyl-CoA hydrolase activity of ACOT8 [peroxisomal matrix]", "activity_id": "390301", "ec_numbers": [ "3.1.2.1" ], "accessions": [ "O14734" ] } ], "citations": [ { "id": "PMID:10092594", "title": "Identification of peroxisomal acyl-CoA thioesterases in yeast and humans", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10092594/" }, { "id": "PMID:16756494", "title": "Biochemistry of mammalian peroxisomes revisited", "journal": "Annu Rev Biochem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16756494/" } ], "x": 868.6666666666667, "y": 1555.6315789473683, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390347", "label": "Exchange of isocitrate and 2-oxoglutarate across the peroxisomal membrane", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "peroxisomal membrane", "compartment_ids": [ "GO:0005778" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-390347", "summary": "Exchange of isocitrate and 2-oxoglutarate across the peroxisomal membrane is a Reactome release 97 curated reaction event. The source record connects 2OG, ISCIT to 2OG, ISCIT in peroxisomal membrane.", "equation": "2OG + ISCIT → 2OG + ISCIT", "inputs": [ { "node_id": "R-ALL-389537", "reactome_id": "R-ALL-389537", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389545", "reactome_id": "R-ALL-389545", "label": "ISCIT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15562", "formula": "C6H5O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389548", "reactome_id": "R-ALL-389548", "label": "ISCIT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15562", "formula": "C6H5O7", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carboxylic acid transmembrane transporter activity of isocitrate-oxoglutarate transporter [peroxisomal membrane]", "activity_id": "390348", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:16919238", "title": "First identification of a 2-ketoglutarate/isocitrate transport system in mammalian peroxisomes and its characterization", "journal": "Biochem Biophys Res Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16919238/" } ], "x": 5008.666666666667, "y": 3460.2916666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-390393", "label": "Peroxisomal uptake of very long-chain fatty acyl CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal membrane", "compartment_ids": [ "GO:0005778" ], "rhea_ids": [ "RHEA:67073", "RHEA:67081" ], "source_url": "https://reactome.org/content/detail/R-HSA-390393", "summary": "Peroxisomal uptake of very long-chain fatty acyl CoA is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects VLCFA-CoA, H2O, ATP to VLCFA, ADP, CoA-SH in peroxisomal membrane and cross-references RHEA:67073, RHEA:67081.", "equation": "VLCFA-CoA + 2 H2O + ATP → VLCFA + ADP + CoA-SH + 2 H+", "inputs": [ { "node_id": "R-ALL-5695971", "reactome_id": "R-ALL-5695971", "label": "VLCFA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:138261", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9914044", "reactome_id": "R-ALL-9914044", "label": "VLCFA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58950", "formula": "CO2R", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ATPase-coupled transmembrane transporter activity of ABCD1 homodimer [peroxisomal membrane]", "activity_id": "390390", "ec_numbers": [ "7.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:21145416", "title": "Differential substrate specificities of human ABCD1 and ABCD2 in peroxisomal fatty acid β-oxidation", "journal": "Biochim Biophys Acta", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21145416/" }, { "id": "PMID:33500543", "title": "Acyl-CoA thioesterase activity of peroxisomal ABC protein ABCD1 is required for the transport of very long-chain acyl-CoA into peroxisomes", "journal": "Sci Rep", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33500543/" }, { "id": "PMID:22493507", "title": "Peroxisomal fatty acid uptake mechanism in Saccharomyces cerevisiae", "journal": "J Biol Chem", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22493507/" } ], "x": 1251.3333333333333, "y": 2286.333333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-4167501", "label": "An unknown protease degrades ACACB", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-4167501", "summary": "An unknown protease degrades ACACB is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Btn-ACACB:2Mn2+ to BCTN in cytosol · mitochondrial outer membrane.", "equation": "Btn-ACACB:2Mn2+ → BCTN", "inputs": [ { "node_id": "R-HSA-2993829", "reactome_id": "R-HSA-2993829", "label": "Btn-ACACB:2Mn2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-3065957", "reactome_id": "R-ALL-3065957", "label": "BCTN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195545", "formula": "C16H28N4O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "peptidase activity of unknown peptidase [cytosol]", "activity_id": "3076897", "ec_numbers": [ "3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:10064314", "title": "Human biotinidase isn't just for recycling biotin", "journal": "J. Nutr.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10064314/" }, { "id": "PMID:8930409", "title": "Biotinidase and its roles in biotin metabolism", "journal": "Clin. Chim. Acta", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8930409/" }, { "id": "PMID:2868710", "title": "Distribution and degradation of biotin-containing carboxylases in human cell lines", "journal": "Biochem. J.", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2868710/" } ], "x": 5948.666666666667, "y": 3166.407894736842, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-4167509", "label": "Mitochondrial BTD hydrolyses BCTN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:77172" ], "source_url": "https://reactome.org/content/detail/R-HSA-4167509", "summary": "Mitochondrial BTD hydrolyses BCTN is a Reactome release 97 curated reaction event. The source record connects BCTN to Btn, L-Lys in mitochondrial matrix and cross-references RHEA:77172.", "equation": "BCTN → Btn + L-Lys", "inputs": [ { "node_id": "R-ALL-3065953", "reactome_id": "R-ALL-3065953", "label": "BCTN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195545", "formula": "C16H28N4O4S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29582", "reactome_id": "R-ALL-29582", "label": "Btn", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29444", "reactome_id": "R-ALL-29444", "label": "L-Lys", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32551", "formula": "C6H15N2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "biotinidase activity of BTD [mitochondrial matrix]", "activity_id": "4167500", "ec_numbers": [ "3.5.1.12" ], "accessions": [ "P43251" ] } ], "citations": [ { "id": "PMID:7509806", "title": "Human serum biotinidase. cDNA cloning, sequence, and characterization", "journal": "J. Biol. Chem.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7509806/" }, { "id": "PMID:6883721", "title": "Biotinidase deficiency: the enzymatic defect in late-onset multiple carboxylase deficiency", "journal": "Clin. Chim. Acta", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6883721/" }, { "id": "PMID:16150625", "title": "Evolutionary conservation of biotinidase: implications for the enzyme's structure and subcellular localization", "journal": "Mol. Genet. Metab.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16150625/" } ], "x": 5391.333333333333, "y": 3012.8552631578946, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-4167511", "label": "HLCS biotinylates ACACB", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-4167511", "summary": "HLCS biotinylates ACACB is a Reactome release 97 curated reaction event. The source record connects ACACB:2Mn2+, Btn, ATP to Btn-ACACB:2Mn2+, AMP, PPi in cytosol · mitochondrial outer membrane.", "equation": "ACACB:2Mn2+ + Btn + ATP → Btn-ACACB:2Mn2+ + AMP + PPi", "inputs": [ { "node_id": "R-HSA-2993859", "reactome_id": "R-HSA-2993859", "label": "ACACB:2Mn2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-199207", "reactome_id": "R-ALL-199207", "label": "Btn", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15956", "formula": "C10H16N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-2993829", "reactome_id": "R-HSA-2993829", "label": "Btn-ACACB:2Mn2+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "biotin--[biotin carboxyl-carrier protein] ligase activity of HLCS [cytosol]", "activity_id": "2993868", "ec_numbers": [ "6.3.4.10" ], "accessions": [ "P50747" ] } ], "citations": [ { "id": "PMID:16134170", "title": "Mutations in the holocarboxylase synthetase gene HLCS", "journal": "Hum. Mutat.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16134170/" }, { "id": "PMID:20153287", "title": "Holocarboxylase synthetase: correlation of protein localisation with biological function", "journal": "Arch. Biochem. Biophys.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20153287/" }, { "id": "PMID:9630604", "title": "Identification of holocarboxylase synthetase (HCS) proteins in human placenta", "journal": "Biochim. Biophys. Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9630604/" }, { "id": "PMID:22123817", "title": "Selectivity in post-translational biotin addition to five human carboxylases", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22123817/" }, { "id": "PMID:10677481", "title": "The subcellular localization of acetyl-CoA carboxylase 2", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10677481/" }, { "id": "PMID:7732023", "title": "Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7732023/" } ], "x": 5670, "y": 3074.2763157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-427555", "label": "SLC26A1,2 cotransport SO4(2-), anion from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:73215", "RHEA:72387", "RHEA:72275", "RHEA:75091" ], "source_url": "https://reactome.org/content/detail/R-HSA-427555", "summary": "SLC26A1,2 cotransport SO4(2-), anion from extracellular region to cytosol is a Reactome release 97 curated reaction event. The source record connects SO4(2-), SLC26A1,2 cargo to SO4(2-), SLC26A1,2 cargo in plasma membrane and cross-references RHEA:73215, RHEA:72387, RHEA:72275, RHEA:75091.", "equation": "SO4(2-) + SLC26A1,2 cargo → SO4(2-) + SLC26A1,2 cargo", "inputs": [ { "node_id": "R-ALL-427648", "reactome_id": "R-ALL-427648", "label": "SO4(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9941480", "reactome_id": "R-ALL-9941480", "label": "SLC26A1,2 cargo", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-174375", "reactome_id": "R-ALL-174375", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9941466", "reactome_id": "R-ALL-9941466", "label": "SLC26A1,2 cargo", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "sulfate transmembrane transporter activity of SLC26A1,2 [plasma membrane]", "activity_id": "427619", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12713736", "title": "Characterization of the human sulfate anion transporter (hsat-1) protein and gene (SAT1; SLC26A1)", "journal": "DNA Cell Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12713736/" }, { "id": "PMID:7923357", "title": "The diastrophic dysplasia gene encodes a novel sulfate transporter: positional cloning by fine-structure linkage disequilibrium mapping", "journal": "Cell", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7923357/" }, { "id": "PMID:23506885", "title": "The SLC26 gene family of anion transporters and channels", "journal": "Mol. Aspects Med.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23506885/" }, { "id": "PMID:8300633", "title": "Functional expression cloning of the canalicular sulfate transport system of rat hepatocytes", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8300633/" }, { "id": "PMID:12759755", "title": "The SLC26 gene family of multifunctional anion exchangers", "journal": "Pflugers Arch", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/12759755/" }, { "id": "PMID:11241838", "title": "Mutations in the diastrophic dysplasia sulfate transporter (DTDST) gene (SLC26A2): 22 novel mutations, mutation review, associated skeletal phenotypes, and diagnostic relevance", "journal": "Hum Mutat", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11241838/" } ], "x": 2381.3333333333335, "y": 747.8939393939394, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-428123", "label": "KDSR reduces 3-ketosphingoid", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-428123", "summary": "KDSR reduces 3-ketosphingoid is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H+, NADPH, 3-ketosphingoid to NADP+, sphingoid base in cytosol · endoplasmic reticulum membrane.", "equation": "H+ + NADPH + 3-ketosphingoid → NADP+ + sphingoid base", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9841798", "reactome_id": "R-ALL-9841798", "label": "3-ketosphingoid", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:84411", "formula": "C3H7NO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5279323", "reactome_id": "R-ALL-5279323", "label": "sphingoid base", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35785", "formula": "", "compartment": "cytoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-dehydrosphinganine reductase activity of KDSR [endoplasmic reticulum membrane]", "activity_id": "428165", "ec_numbers": [ "1.1.1.102" ], "accessions": [ "Q06136" ] } ], "citations": [ { "id": "PMID:15328338", "title": "FVT-1 is a mammalian 3-ketodihydrosphingosine reductase with an active site that faces the cytosolic side of the endoplasmic reticulum membrane", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15328338/" }, { "id": "PMID:28575652", "title": "Mutations in KDSR Cause Recessive Progressive Symmetric Erythrokeratoderma", "journal": "Am J Hum Genet", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28575652/" } ], "x": 3321.3333333333335, "y": 1705.0576923076924, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428127", "label": "SPTLC complexes transfer acyl-CoA onto serine", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-428127", "summary": "SPTLC complexes transfer acyl-CoA onto serine is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects L-Ser, acyl-CoA, H+ to CoA-SH, CO2, 3-ketosphingoid in cytosol · endoplasmic reticulum membrane.", "equation": "L-Ser + acyl-CoA + H+ → CoA-SH + CO2 + 3-ketosphingoid", "inputs": [ { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9841798", "reactome_id": "R-ALL-9841798", "label": "3-ketosphingoid", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84411", "formula": "C3H7NO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "serine C-palmitoyltransferase activity of SPTLC complexes [endoplasmic reticulum membrane]", "activity_id": "428142", "ec_numbers": [ "2.3.1.50" ], "accessions": [] } ], "citations": [ { "id": "PMID:17023427", "title": "Cloning and initial characterization of a new subunit for mammalian serine-palmitoyltransferase", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17023427/" }, { "id": "PMID:9363775", "title": "Human and murine serine-palmitoyl-CoA transferase--cloning, expression and characterization of the key enzyme in sphingolipid synthesis", "journal": "Eur J Biochem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9363775/" }, { "id": "PMID:17331073", "title": "Is the mammalian serine palmitoyltransferase a high-molecular-mass complex?", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17331073/" }, { "id": "PMID:10722674", "title": "Purification of the serine palmitoyltransferase complex responsible for sphingoid base synthesis by using affinity peptide chromatography techniques", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10722674/" }, { "id": "PMID:19416851", "title": "Identification of small subunits of mammalian serine palmitoyltransferase that confer distinct acyl-CoA substrate specificities", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19416851/" }, { "id": "PMID:20182505", "title": "Orm family proteins mediate sphingolipid homeostasis", "journal": "Nature", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20182505/" } ], "x": 3878.666666666667, "y": 1660.173076923077, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428185", "label": "Ceramide synthases transfer acyl-CoA onto sphingoid", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-428185", "summary": "Ceramide synthases transfer acyl-CoA onto sphingoid is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects acyl-CoA, sphingoid base to DHCE, CoA-SH, H+ in cytosol · endoplasmic reticulum membrane.", "equation": "acyl-CoA + sphingoid base → DHCE + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5279323", "reactome_id": "R-ALL-5279323", "label": "sphingoid base", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35785", "formula": "", "compartment": "cytoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-428126", "reactome_id": "R-ALL-428126", "label": "DHCE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:139048", "formula": "C5H9NO3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sphingosine N-acyltransferase activity of Ceramide synthases [endoplasmic reticulum membrane]", "activity_id": "428183", "ec_numbers": [ "2.3.1.24" ], "accessions": [] } ], "citations": [ { "id": "PMID:16793762", "title": "When do Lasses (longevity assurance genes) become CerS (ceramide synthases)?: Insights into the regulation of ceramide synthesis", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16793762/" }, { "id": "PMID:16100120", "title": "LASS5 is a bona fide dihydroceramide synthase that selectively utilizes palmitoyl-CoA as acyl donor", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16100120/" }, { "id": "PMID:18165233", "title": "Characterization of ceramide synthase 2: tissue distribution, substrate specificity, and inhibition by sphingosine 1-phosphate", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18165233/" }, { "id": "PMID:22248339", "title": "Ceramide synthases at the centre of sphingolipid metabolism and biology", "journal": "Biochem J", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22248339/" }, { "id": "PMID:26248326", "title": "Ceramide synthases in biomedical research", "journal": "Chem Phys Lipids", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26248326/" }, { "id": "PMID:20919646", "title": "Ceramide synthases: roles in cell physiology and signaling", "journal": "Adv Exp Med Biol", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20919646/" } ], "x": 3600, "y": 1705.0576923076924, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428205", "label": "ACER2 hydrolyzes ceramide (Golgi)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005829" ], "rhea_ids": [ "RHEA:78455" ], "source_url": "https://reactome.org/content/detail/R-HSA-428205", "summary": "ACER2 hydrolyzes ceramide (Golgi) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, CERA to RCOO-, SPG in Golgi membrane · cytosol and cross-references RHEA:78455.", "equation": "H2O + CERA → RCOO- + SPG", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428270", "reactome_id": "R-ALL-428270", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1861754", "reactome_id": "R-ALL-1861754", "label": "RCOO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28868", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428682", "reactome_id": "R-ALL-428682", "label": "SPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84410", "formula": "C3H9NO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acylsphingosine amidohydrolase activity of ACER2 [Golgi membrane]", "activity_id": "428257", "ec_numbers": [ "3.5.1.23" ], "accessions": [ "Q5QJU3" ] } ], "citations": [ { "id": "PMID:16940153", "title": "Golgi alkaline ceramidase regulates cell proliferation and survival by controlling levels of sphingosine and S1P", "journal": "FASEB J", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16940153/" }, { "id": "PMID:18619555", "title": "Ceramidases: regulators of cellular responses mediated by ceramide, sphingosine, and sphingosine-1-phosphate", "journal": "Biochim Biophys Acta", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18619555/" }, { "id": "PMID:32498325", "title": "Elusive Roles of the Different Ceramidases in Human Health, Pathophysiology, and Tissue Regeneration", "journal": "Cells", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32498325/" }, { "id": "PMID:31817238", "title": "Role of Ceramidases in Sphingolipid Metabolism and Human Diseases", "journal": "Cells", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31817238/" }, { "id": "PMID:20089856", "title": "Substrate specificity, membrane topology, and activity regulation of human alkaline ceramidase 2 (ACER2)", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20089856/" }, { "id": "PMID:29229990", "title": "Tumor suppressor p53 links ceramide metabolism to DNA damage response through alkaline ceramidase 2", "journal": "Cell Death Differ", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29229990/" } ], "x": 3321.3333333333335, "y": 1615.2884615384614, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428231", "label": "ACER1 hydrolyzes ceramide (endoplasmic reticulum)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:78455" ], "source_url": "https://reactome.org/content/detail/R-HSA-428231", "summary": "ACER1 hydrolyzes ceramide (endoplasmic reticulum) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, CERA to RCOO-, SPG in cytosol · endoplasmic reticulum membrane and cross-references RHEA:78455.", "equation": "H2O + CERA → RCOO- + SPG", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428256", "reactome_id": "R-ALL-428256", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1861754", "reactome_id": "R-ALL-1861754", "label": "RCOO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28868", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428682", "reactome_id": "R-ALL-428682", "label": "SPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84410", "formula": "C3H9NO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acylsphingosine amidohydrolase activity of ACER1 [endoplasmic reticulum membrane]", "activity_id": "428216", "ec_numbers": [ "3.5.1.23" ], "accessions": [ "Q8TDN7" ] } ], "citations": [ { "id": "PMID:17713573", "title": "Upregulation of the human alkaline ceramidase 1 and acid ceramidase mediates calcium-induced differentiation of epidermal keratinocytes", "journal": "J Invest Dermatol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17713573/" }, { "id": "PMID:18619555", "title": "Ceramidases: regulators of cellular responses mediated by ceramide, sphingosine, and sphingosine-1-phosphate", "journal": "Biochim Biophys Acta", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18619555/" }, { "id": "PMID:32498325", "title": "Elusive Roles of the Different Ceramidases in Human Health, Pathophysiology, and Tissue Regeneration", "journal": "Cells", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32498325/" }, { "id": "PMID:31817238", "title": "Role of Ceramidases in Sphingolipid Metabolism and Human Diseases", "journal": "Cells", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31817238/" }, { "id": "PMID:26286360", "title": "Activity of neutral and alkaline ceramidases on fluorogenic N-acylated coumarin-containing aminodiols", "journal": "J Lipid Res", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26286360/" }, { "id": "PMID:27126290", "title": "Alkaline ceramidase 1 is essential for mammalian skin homeostasis and regulating whole-body energy expenditure", "journal": "J Pathol", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27126290/" } ], "x": 3600, "y": 1615.2884615384614, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428259", "label": "DEGS1 dehydrogenates dihydroceramide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-428259", "summary": "DEGS1 dehydrogenates dihydroceramide is a Reactome release 97 curated reaction event. The source record connects H+, O2, DHCE to H2O, (4E)-CERA, CYB5B:Fe3+ in cytosol · endoplasmic reticulum membrane.", "equation": "2 H+ + O2 + DHCE + 2 CYB5B:Fe2+ → 2 H2O + (4E)-CERA + 2 CYB5B:Fe3+", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428126", "reactome_id": "R-ALL-428126", "label": "DHCE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:139048", "formula": "C5H9NO3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9842697", "reactome_id": "R-HSA-9842697", "label": "CYB5B:Fe2+", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9842380", "reactome_id": "R-ALL-9842380", "label": "(4E)-CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195634", "formula": "C6H9NO3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9842694", "reactome_id": "R-HSA-9842694", "label": "CYB5B:Fe3+", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "sphingolipid delta-4 desaturase activity of DEGS1 [endoplasmic reticulum membrane]", "activity_id": "428230", "ec_numbers": [ "1.14.19.17" ], "accessions": [ "O15121" ] } ], "citations": [ { "id": "PMID:9312549", "title": "Characterization of ceramide synthesis. A dihydroceramide desaturase introduces the 4,5-trans-double bond of sphingosine at the level of dihydroceramide", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9312549/" }, { "id": "PMID:11937514", "title": "Identification and characterization of a sphingolipid delta 4-desaturase family", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11937514/" }, { "id": "PMID:9188692", "title": "The product of the MLD gene is a member of the membrane fatty acid desaturase family: overexpression of MLD inhibits EGF receptor biosynthesis", "journal": "Biochemistry", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9188692/" }, { "id": "PMID:22200621", "title": "Dihydroceramide desaturase and dihydrosphingolipids: debutant players in the sphingolipid arena", "journal": "Prog Lipid Res", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22200621/" }, { "id": "PMID:9355743", "title": "Conversion of dihydroceramide into ceramide: involvement of a desaturase", "journal": "Biochem J", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9355743/" }, { "id": "PMID:37195341", "title": "DEGS1 -related leukodystrophy: a clinical report and review of literature", "journal": "Clin Dysmorphol", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37195341/" } ], "x": 3878.666666666667, "y": 1705.0576923076924, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-428260", "label": "DEGS2 oxygenates dihydroceramide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-428260", "summary": "DEGS2 oxygenates dihydroceramide is a Reactome release 97 curated reaction event. The source record connects H+, O2, DHCE to H2O, phytoceramide, CYB5B:Fe3+ in cytosol · endoplasmic reticulum membrane.", "equation": "H+ + O2 + DHCE + CYB5B:Fe2+ → H2O + phytoceramide + CYB5B:Fe3+", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428126", "reactome_id": "R-ALL-428126", "label": "DHCE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:139048", "formula": "C5H9NO3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9842697", "reactome_id": "R-HSA-9842697", "label": "CYB5B:Fe2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9025544", "reactome_id": "R-ALL-9025544", "label": "phytoceramide", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:139051", "formula": "C5H9NO4R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9842694", "reactome_id": "R-HSA-9842694", "label": "CYB5B:Fe3+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "sphingolipid C4-monooxygenase activity of DEGS2 [endoplasmic reticulum membrane]", "activity_id": "428238", "ec_numbers": [ "1.14.18.5" ], "accessions": [ "Q6QHC5" ] } ], "citations": [ { "id": "PMID:15063729", "title": "Identification of the human sphingolipid C4-hydroxylase, hDES2, and its up-regulation during keratinocyte differentiation", "journal": "FEBS Lett", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15063729/" }, { "id": "PMID:36907437", "title": "Bifunctional DEGS2 has higher hydroxylase activity toward substrates with very-long-chain fatty acids in the production of phytosphingosine ceramides", "journal": "J Biol Chem", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36907437/" } ], "x": 3321.3333333333335, "y": 1720.0192307692307, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-428262", "label": "ACER3 hydrolyzes phytoceramide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:33555" ], "source_url": "https://reactome.org/content/detail/R-HSA-428262", "summary": "ACER3 hydrolyzes phytoceramide is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, phytoceramide-C16 to phytosphingosine, RCOO- in cytosol · endoplasmic reticulum membrane and cross-references RHEA:33555.", "equation": "H2O + phytoceramide-C16 → phytosphingosine + RCOO-", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9843855", "reactome_id": "R-ALL-9843855", "label": "phytoceramide-C16", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:31998", "formula": "C19H38NO4R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-428237", "reactome_id": "R-ALL-428237", "label": "phytosphingosine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64124", "formula": "C18H40NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1861754", "reactome_id": "R-ALL-1861754", "label": "RCOO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28868", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acylsphingosine amidohydrolase activity of ACER3:Ca2+:Zn2+ [endoplasmic reticulum membrane]", "activity_id": "428234", "ec_numbers": [ "3.5.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:11356846", "title": "Cloning and characterization of a novel human alkaline ceramidase. A mammalian enzyme that hydrolyzes phytoceramide", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11356846/" }, { "id": "PMID:18619555", "title": "Ceramidases: regulators of cellular responses mediated by ceramide, sphingosine, and sphingosine-1-phosphate", "journal": "Biochim Biophys Acta", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18619555/" }, { "id": "PMID:32498325", "title": "Elusive Roles of the Different Ceramidases in Human Health, Pathophysiology, and Tissue Regeneration", "journal": "Cells", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32498325/" }, { "id": "PMID:31817238", "title": "Role of Ceramidases in Sphingolipid Metabolism and Human Diseases", "journal": "Cells", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31817238/" }, { "id": "PMID:30575723", "title": "Structure of a human intramembrane ceramidase explains enzymatic dysfunction found in leukodystrophy", "journal": "Nat Commun", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30575723/" }, { "id": "PMID:26286360", "title": "Activity of neutral and alkaline ceramidases on fluorogenic N-acylated coumarin-containing aminodiols", "journal": "J Lipid Res", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26286360/" } ], "x": 3878.666666666667, "y": 1615.2884615384614, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428273", "label": "SPHK1 phosphorylates sphingoid", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:51496" ], "source_url": "https://reactome.org/content/detail/R-HSA-428273", "summary": "SPHK1 phosphorylates sphingoid is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATP, SPG to ADP, S1P, H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:51496.", "equation": "ATP + SPG → ADP + S1P + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428682", "reactome_id": "R-ALL-428682", "label": "SPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:84410", "formula": "C3H9NO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5279322", "reactome_id": "R-ALL-5279322", "label": "S1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76941", "formula": "C3H8NO5PR", "compartment": "cytoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "lipid kinase activity of SPHK1 [cytosol]", "activity_id": "428281", "ec_numbers": null, "accessions": [ "Q9NYA1" ] } ], "citations": [ { "id": "PMID:10802064", "title": "Functional characterization of human sphingosine kinase-1", "journal": "FEBS Lett", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10802064/" }, { "id": "PMID:10947957", "title": "Human sphingosine kinase: purification, molecular cloning and characterization of the native and recombinant enzymes", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10947957/" }, { "id": "PMID:28415564", "title": "Mammalian sphingosine kinase (SphK) isoenzymes and isoform expression: challenges for SphK as an oncotarget", "journal": "Oncotarget", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28415564/" }, { "id": "PMID:23602659", "title": "Molecular basis of sphingosine kinase 1 substrate recognition and catalysis", "journal": "Structure", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23602659/" }, { "id": "PMID:21864225", "title": "The compartmentalization and translocation of the sphingosine kinases: mechanisms and functions in cell signaling and sphingolipid metabolism", "journal": "Crit Rev Biochem Mol Biol", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21864225/" }, { "id": "PMID:33171624", "title": "Transcriptional Regulation of Sphingosine Kinase 1", "journal": "Cells", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33171624/" } ], "x": 3321.3333333333335, "y": 1570.4038461538462, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428664", "label": "SGPP1,2 dephosphorylate sphingoid-1-phosphates", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:63952" ], "source_url": "https://reactome.org/content/detail/R-HSA-428664", "summary": "SGPP1,2 dephosphorylate sphingoid-1-phosphates is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, S1P to Pi, SPG in cytosol · endoplasmic reticulum membrane and cross-references RHEA:63952.", "equation": "H2O + S1P → Pi + SPG", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5279322", "reactome_id": "R-ALL-5279322", "label": "S1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:76941", "formula": "C3H8NO5PR", "compartment": "cytoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428682", "reactome_id": "R-ALL-428682", "label": "SPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84410", "formula": "C3H9NO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sphingosine-1-phosphate phosphatase activity of SGPP [endoplasmic reticulum membrane]", "activity_id": "428700", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12411432", "title": "Identification and characterization of a novel human sphingosine-1-phosphate phosphohydrolase, hSPP2", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12411432/" }, { "id": "PMID:12815058", "title": "Role of human sphingosine-1-phosphate phosphatase 1 in the regulation of intra- and extracellular sphingosine-1-phosphate levels and cell viability", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12815058/" }, { "id": "PMID:16782891", "title": "Sphingosine-1-phosphate phosphohydrolase regulates endoplasmic reticulum-to-golgi trafficking of ceramide", "journal": "Mol Cell Biol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16782891/" } ], "x": 3321.3333333333335, "y": 1630.25, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428681", "label": "PXLP-SGPL1 cleaves sphingoid-1-phosphates", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:18594", "RHEA:33508" ], "source_url": "https://reactome.org/content/detail/R-HSA-428681", "summary": "PXLP-SGPL1 cleaves sphingoid-1-phosphates is a Reactome release 97 curated reaction event. The source record connects S1P,SPAP to PETA, HXAL,HD2NAL in cytosol · endoplasmic reticulum membrane and cross-references RHEA:18594, RHEA:33508.", "equation": "S1P,SPAP → PETA + HXAL,HD2NAL", "inputs": [ { "node_id": "R-ALL-9844328", "reactome_id": "R-ALL-9844328", "label": "S1P,SPAP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-428686", "reactome_id": "R-ALL-428686", "label": "PETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17553", "formula": "C2H8NO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9844327", "reactome_id": "R-ALL-9844327", "label": "HXAL,HD2NAL", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "sphinganine-1-phosphate aldolase activity of PXLP-SGPL1 [endoplasmic reticulum membrane]", "activity_id": "428675", "ec_numbers": [ "4.1.2.27" ], "accessions": [ "O95470" ] } ], "citations": [ { "id": "PMID:11018465", "title": "Human sphingosine-1-phosphate lyase: cDNA cloning, functional expression studies and mapping to chromosome 10q22(1)", "journal": "Biochim Biophys Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11018465/" }, { "id": "PMID:18558101", "title": "Sphingosine-1-phosphate lyase in development and disease: sphingolipid metabolism takes flight", "journal": "Biochim Biophys Acta", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18558101/" }, { "id": "PMID:30090628", "title": "Nephrotic syndrome and adrenal insufficiency caused by a variant in SGPL1", "journal": "Clin Kidney J", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30090628/" }, { "id": "PMID:30635364", "title": "Fifty years of lyase and a moment of truth: sphingosine phosphate lyase from discovery to disease", "journal": "J Lipid Res", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30635364/" } ], "x": 3600, "y": 1540.4807692307693, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-428690", "label": "PLPP1 dephosphorylates extracellular sphingosine-1-phosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:27518" ], "source_url": "https://reactome.org/content/detail/R-HSA-428690", "summary": "PLPP1 dephosphorylates extracellular sphingosine-1-phosphate is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, C18-S1P to Pi, sphingosine in extracellular region · plasma membrane and cross-references RHEA:27518.", "equation": "H2O + C18-S1P → Pi + sphingosine", "inputs": [ { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-419305", "reactome_id": "R-ALL-419305", "label": "C18-S1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60119", "formula": "C18H37NO5P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1132148", "reactome_id": "R-ALL-1132148", "label": "sphingosine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57756", "formula": "C18H38NO2", "compartment": "cytoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sphingosine-1-phosphate phosphatase activity of PLPP1 [plasma membrane]", "activity_id": "428666", "ec_numbers": null, "accessions": [ "O14494" ] } ], "citations": [ { "id": "PMID:9705349", "title": "Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9705349/" }, { "id": "PMID:17379599", "title": "Intracellular generation of sphingosine 1-phosphate in human lung endothelial cells: role of lipid phosphate phosphatase-1 and sphingosine kinase 1", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17379599/" }, { "id": "PMID:25814022", "title": "Lipid phosphate phosphatases and their roles in mammalian physiology and pathology", "journal": "J Lipid Res", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25814022/" } ], "x": 3600, "y": 1630.25, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428696", "label": "PLPP1,2,3 dephosphorylate cytosolic sphingosine-1-phosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [ "RHEA:27518" ], "source_url": "https://reactome.org/content/detail/R-HSA-428696", "summary": "PLPP1,2,3 dephosphorylate cytosolic sphingosine-1-phosphate is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, C18-S1P to Pi, sphingosine in cytosol · plasma membrane and cross-references RHEA:27518.", "equation": "H2O + C18-S1P → Pi + sphingosine", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428203", "reactome_id": "R-ALL-428203", "label": "C18-S1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60119", "formula": "C18H37NO5P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1132148", "reactome_id": "R-ALL-1132148", "label": "sphingosine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57756", "formula": "C18H38NO2", "compartment": "cytoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sphingosine-1-phosphate phosphatase activity of PLPP1,2,3 [plasma membrane]", "activity_id": "428693", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9705349", "title": "Human type 2 phosphatidic acid phosphohydrolases. Substrate specificity of the type 2a, 2b, and 2c enzymes and cell surface activity of the 2a isoform.", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9705349/" }, { "id": "PMID:25814022", "title": "Lipid phosphate phosphatases and their roles in mammalian physiology and pathology", "journal": "J Lipid Res", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25814022/" }, { "id": "PMID:32887262", "title": "Lipid Phosphate Phosphatases and Cancer", "journal": "Biomolecules", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32887262/" } ], "x": 3878.666666666667, "y": 1630.25, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-428990", "label": "SLC27A1 transports arachidonate across the ER membrane", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen · cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:71395" ], "source_url": "https://reactome.org/content/detail/R-HSA-428990", "summary": "SLC27A1 transports arachidonate across the ER membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects AA to AA in endoplasmic reticulum lumen · cytosol · endoplasmic reticulum membrane and cross-references RHEA:71395.", "equation": "AA → AA", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "icosanoid transmembrane transporter activity of SLC27A1 [endoplasmic reticulum membrane]", "activity_id": "9915567", "ec_numbers": null, "accessions": [ "Q6PCB7" ] } ], "citations": [ { "id": "PMID:15897321", "title": "Impact on fatty acid metabolism and differential localization of FATP1 and FAT/CD36 proteins delivered in cultured human muscle cells", "journal": "Am J Physiol Cell Physiol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15897321/" }, { "id": "PMID:6810878", "title": "How is the level of free arachidonic acid controlled in mammalian cells?", "journal": "Biochem J", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6810878/" }, { "id": "PMID:7954810", "title": "Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein", "journal": "Cell", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7954810/" } ], "x": 311.33333333333337, "y": 1634.0877192982457, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-429016", "label": "ALOX5 is phosphorylated by MAPKAP2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17990" ], "source_url": "https://reactome.org/content/detail/R-HSA-429016", "summary": "ALOX5 is phosphorylated by MAPKAP2 is a Reactome release 97 curated reaction event. The source record connects ALOX5:Ca2+:Fe2+, ATP to p-S272-ALOX5:Ca2+:Fe2+, ADP, H+ in cytosol and cross-references RHEA:17990.", "equation": "ALOX5:Ca2+:Fe2+ + ATP → p-S272-ALOX5:Ca2+:Fe2+ + ADP + H+", "inputs": [ { "node_id": "R-HSA-2237880", "reactome_id": "R-HSA-2237880", "label": "ALOX5:Ca2+:Fe2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-265277", "reactome_id": "R-HSA-265277", "label": "p-S272-ALOX5:Ca2+:Fe2+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein serine/threonine kinase activity of p-S272,T222,T334-MAPKAPK2 [cytosol]", "activity_id": "429015", "ec_numbers": [ "2.7.11.1" ], "accessions": [ "P49137" ] } ], "citations": [ { "id": "PMID:10779545", "title": "5-lipoxygenase is phosphorylated by p38 kinase-dependent MAPKAP kinases", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10779545/" }, { "id": "PMID:11844797", "title": "Arachidonic acid promotes phosphorylation of 5-lipoxygenase at Ser-271 by MAPK-activated protein kinase 2 (MK2)", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11844797/" } ], "x": 590, "y": 1685.3859649122808, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-429698", "label": "PRKD1,2,3 phosphorylates CERT1-2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-429698", "summary": "PRKD1,2,3 phosphorylates CERT1-2 is a Reactome release 97 curated reaction event. The source record connects ATP, CERT1-2 to ADP, p-S132-CERT1-2, H+ in cytosol.", "equation": "ATP + CERT1-2 → ADP + p-S132-CERT1-2 + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-429712", "reactome_id": "R-HSA-429712", "label": "CERT1-2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-429704", "reactome_id": "R-HSA-429704", "label": "p-S132-CERT1-2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diacylglycerol-dependent, calcium-independent serine/threonine kinase activity of PRKD1,2,3 [cytosol]", "activity_id": "8874351", "ec_numbers": [ "2.7.11.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:17591919", "title": "Regulation of secretory transport by protein kinase D-mediated phosphorylation of the ceramide transfer protein", "journal": "J Cell Biol", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17591919/" }, { "id": "PMID:35409383", "title": "Hyperosmotic Stress Induces Phosphorylation of CERT and Enhances Its Tethering throughout the Endoplasmic Reticulum", "journal": "Int J Mol Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35409383/" }, { "id": "PMID:31254361", "title": "Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites", "journal": "FEBS Lett", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31254361/" }, { "id": "PMID:24569996", "title": "Phosphoregulation of the ceramide transport protein CERT at serine 315 in the interaction with VAMP-associated protein (VAP) for inter-organelle trafficking of ceramide in mammalian cells", "journal": "J Biol Chem", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24569996/" }, { "id": "PMID:22226883", "title": "Integration of non-vesicular and vesicular transport processes at the Golgi complex by the PKD-CERT network", "journal": "Biochim Biophys Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22226883/" } ], "x": 3600, "y": 1779.8653846153845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-429714", "label": "CSNK1G2 phosphorylates p-CERT1-2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-429714", "summary": "CSNK1G2 phosphorylates p-CERT1-2 is a Reactome release 97 curated reaction event. The source record connects ATP, p-S132-CERT1-2 to ADP, p-S,S132,T-CERT1-2, H+ in cytosol.", "equation": "2 ATP + p-S132-CERT1-2 → 2 ADP + p-S,S132,T-CERT1-2 + 2 H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-429704", "reactome_id": "R-HSA-429704", "label": "p-S132-CERT1-2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-429695", "reactome_id": "R-HSA-429695", "label": "p-S,S132,T-CERT1-2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein serine/threonine kinase activity of CSNK1G2 [cytosol]", "activity_id": "429721", "ec_numbers": [ "2.7.11.1" ], "accessions": [ "P78368" ] } ], "citations": [ { "id": "PMID:19005213", "title": "Casein kinase I{gamma}2 down-regulates trafficking of ceramide in the synthesis of sphingomyelin", "journal": "Mol Biol Cell", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19005213/" }, { "id": "PMID:31254361", "title": "Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites", "journal": "FEBS Lett", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31254361/" } ], "x": 3878.666666666667, "y": 1779.8653846153845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-429730", "label": "PPM1L dephosphorylates multiphospho-CERT1-2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:20630", "RHEA:47005" ], "source_url": "https://reactome.org/content/detail/R-HSA-429730", "summary": "PPM1L dephosphorylates multiphospho-CERT1-2 is a Reactome release 97 curated reaction event. The source record connects H2O, PPM1L:VAPA/B dimer, p-S,S132,T-CERT1-2 to CERT1-2:PPM1L:VAPA/B dimer, Pi in cytosol · endoplasmic reticulum membrane and cross-references RHEA:20630, RHEA:47005.", "equation": "3 H2O + PPM1L:VAPA/B dimer + p-S,S132,T-CERT1-2 → CERT1-2:PPM1L:VAPA/B dimer + 3 Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-429703", "reactome_id": "R-HSA-429703", "label": "PPM1L:VAPA/B dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-429695", "reactome_id": "R-HSA-429695", "label": "p-S,S132,T-CERT1-2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-429672", "reactome_id": "R-HSA-429672", "label": "CERT1-2:PPM1L:VAPA/B dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "protein serine/threonine phosphatase activity of PPM1L:VAPA/B dimer [endoplasmic reticulum membrane]", "activity_id": "429724", "ec_numbers": [ "3.1.3.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:18165232", "title": "Protein phosphatase 2Cepsilon is an endoplasmic reticulum integral membrane protein that dephosphorylates the ceramide transport protein CERT to enhance its association with organelle membranes", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18165232/" }, { "id": "PMID:31254361", "title": "Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites", "journal": "FEBS Lett", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31254361/" } ], "x": 3321.3333333333335, "y": 1540.4807692307693, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-429749", "label": "SLC5A8 (SMCT) co-transports monocarboxylates and Na+ from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane · cytosol · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005829", "GO:0005576" ], "rhea_ids": [ "RHEA:75803", "RHEA:75791", "RHEA:75799", "RHEA:75807", "RHEA:27814", "RHEA:72935", "RHEA:75795", "RHEA:72947", "RHEA:72951", "RHEA:72939", "RHEA:72943", "RHEA:72955" ], "source_url": "https://reactome.org/content/detail/R-HSA-429749", "summary": "SLC5A8 (SMCT) co-transports monocarboxylates and Na+ from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects monocarboxylates transported by SLC5A8, Na+ to monocarboxylates transported by SLC5A8, Na+ in plasma membrane · cytosol · extracellular region and cross-references RHEA:75803, RHEA:75791, RHEA:75799, RHEA:75807, RHEA:27814, RHEA:72935, RHEA:75795, RHEA:72947, RHEA:72951, RHEA:72939, RHEA:72943, RHEA:72955.", "equation": "monocarboxylates transported by SLC5A8 + 2 Na+ → monocarboxylates transported by SLC5A8 + 2 Na+", "inputs": [ { "node_id": "R-ALL-429739", "reactome_id": "R-ALL-429739", "label": "monocarboxylates transported by SLC5A8", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-74113", "reactome_id": "R-ALL-74113", "label": "Na+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-429748", "reactome_id": "R-ALL-429748", "label": "monocarboxylates transported by SLC5A8", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-83910", "reactome_id": "R-ALL-83910", "label": "Na+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monocarboxylic acid transmembrane transporter activity of SLC5A8 [plasma membrane]", "activity_id": "429740", "ec_numbers": null, "accessions": [ "Q8N695" ] } ], "citations": [ { "id": "PMID:15090606", "title": "The human tumour suppressor gene SLC5A8 expresses a Na+-monocarboxylate cotransporter", "journal": "J Physiol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15090606/" }, { "id": "PMID:33353981", "title": "NAD + metabolism and its roles in cellular processes during ageing", "journal": "Nat Rev Mol Cell Biol", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33353981/" }, { "id": "PMID:14966140", "title": "Functional identification of SLC5A8, a tumor suppressor down-regulated in colon cancer, as a Na(+)-coupled transporter for short-chain fatty acids", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14966140/" } ], "x": 5391.333333333333, "y": 2808.1184210526317, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-429786", "label": "SGMS2 transfers phosphocholine onto ceramide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:81452" ], "source_url": "https://reactome.org/content/detail/R-HSA-429786", "summary": "SGMS2 transfers phosphocholine onto ceramide is a Reactome release 97 curated reaction event. The source record connects PC, CERA to SPHM, DAG in plasma membrane and cross-references RHEA:81452.", "equation": "PC + CERA → SPHM + DAG", "inputs": [ { "node_id": "R-ALL-429802", "reactome_id": "R-ALL-429802", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-429807", "reactome_id": "R-ALL-429807", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9843210", "reactome_id": "R-ALL-9843210", "label": "SPHM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64583", "formula": "C9H19N2O6PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-114519", "reactome_id": "R-ALL-114519", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sphingomyelin synthase activity of 2xPalmC-SGMS2 [plasma membrane]", "activity_id": "429800", "ec_numbers": [ "2.7.8.27" ], "accessions": [ "Q8NHU3" ] } ], "citations": [ { "id": "PMID:17449912", "title": "Both sphingomyelin synthases SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17449912/" }, { "id": "PMID:14685263", "title": "Identification of a family of animal sphingomyelin synthases", "journal": "EMBO J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14685263/" }, { "id": "PMID:19233134", "title": "Sphingomyelin synthase 2 is palmitoylated at the COOH-terminal tail, which is involved in its localization in plasma membranes", "journal": "Biochem Biophys Res Commun", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19233134/" }, { "id": "PMID:4339164", "title": "Evidence for a new biosynthetic pathway of sphingomyelin in SV 40 transformed mouse cells", "journal": "Biochem Biophys Res Commun", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/4339164/" }, { "id": "PMID:4817756", "title": "The enzymatic formation of sphingomyelin from ceramide and lecithin in mouse liver", "journal": "J Biol Chem", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4817756/" }, { "id": "PMID:17982138", "title": "SMS overexpression and knockdown: impact on cellular sphingomyelin and diacylglycerol metabolism, and cell apoptosis", "journal": "J Lipid Res", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17982138/" } ], "x": 3321.3333333333335, "y": 1645.2115384615386, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-429798", "label": "SGMS1 transfers phosphocholine onto ceramide", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane", "compartment_ids": [ "GO:0000139" ], "rhea_ids": [ "RHEA:81452" ], "source_url": "https://reactome.org/content/detail/R-HSA-429798", "summary": "SGMS1 transfers phosphocholine onto ceramide is a Reactome release 97 curated reaction event. The source record connects PC, CERA to SPHM, DAG in Golgi membrane and cross-references RHEA:81452.", "equation": "PC + CERA → SPHM + DAG", "inputs": [ { "node_id": "R-ALL-429785", "reactome_id": "R-ALL-429785", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428270", "reactome_id": "R-ALL-428270", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-429789", "reactome_id": "R-ALL-429789", "label": "SPHM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64583", "formula": "C9H19N2O6PR2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524057", "reactome_id": "R-ALL-1524057", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sphingomyelin synthase activity of SGMS1 [Golgi membrane]", "activity_id": "429784", "ec_numbers": [ "2.7.8.27" ], "accessions": [ "Q86VZ5" ] } ], "citations": [ { "id": "PMID:17449912", "title": "Both sphingomyelin synthases SMS1 and SMS2 are required for sphingomyelin homeostasis and growth in human HeLa cells", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17449912/" }, { "id": "PMID:14976195", "title": "Expression cloning of a human cDNA restoring sphingomyelin synthesis and cell growth in sphingomyelin synthase-defective lymphoid cells", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14976195/" }, { "id": "PMID:14685263", "title": "Identification of a family of animal sphingomyelin synthases", "journal": "EMBO J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14685263/" }, { "id": "PMID:4339164", "title": "Evidence for a new biosynthetic pathway of sphingomyelin in SV 40 transformed mouse cells", "journal": "Biochem Biophys Res Commun", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/4339164/" }, { "id": "PMID:4817756", "title": "The enzymatic formation of sphingomyelin from ceramide and lecithin in mouse liver", "journal": "J Biol Chem", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4817756/" }, { "id": "PMID:28742512", "title": "The sphingomyelin synthase family: proteins, diseases, and inhibitors", "journal": "Biol Chem", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28742512/" } ], "x": 3600, "y": 1645.2115384615386, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-443894", "label": "TXA2 is hydrolysed to TXB2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-443894", "summary": "TXA2 is hydrolysed to TXB2 is a Reactome release 97 curated reaction event. The source record connects TXA2, H2O to TXB2 in endoplasmic reticulum lumen.", "equation": "TXA2 + H2O → TXB2", "inputs": [ { "node_id": "R-ALL-32879", "reactome_id": "R-ALL-32879", "label": "TXA2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57445", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-443890", "reactome_id": "R-ALL-443890", "label": "TXB2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90696", "formula": "C20H33O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:1059088", "title": "Thromboxanes: a new group of biologically active compounds derived from prostaglandin endoperoxides", "journal": "Proc Natl Acad Sci U S A", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/1059088/" }, { "id": "PMID:11297515", "title": "Substrate binding is the rate-limiting step in thromboxane synthase catalysis", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11297515/" } ], "x": 590, "y": 1739.701754385965, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-444433", "label": "Cho transports from the extracellular space to the cytosol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane · cytosol", "compartment_ids": [ "GO:0005576", "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:32751" ], "source_url": "https://reactome.org/content/detail/R-HSA-444433", "summary": "Cho transports from the extracellular space to the cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects Cho to Cho in extracellular region · plasma membrane · cytosol and cross-references RHEA:32751.", "equation": "Cho → Cho", "inputs": [ { "node_id": "R-ALL-264638", "reactome_id": "R-ALL-264638", "label": "Cho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-264618", "reactome_id": "R-ALL-264618", "label": "Cho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15354", "formula": "C5H14NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "choline transmembrane transporter activity of CTL1-5 [plasma membrane]", "activity_id": "444463", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11698453", "title": "Characterization of CDw92 as a member of the choline transporter-like protein family regulated specifically on dendritic cells", "journal": "J Immunol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11698453/" }, { "id": "PMID:14973250", "title": "Identification and characterization of choline transporter-like protein 2, an inner ear glycoprotein of 68 and 72 kDa that is the target of antibody-induced hearing loss", "journal": "J Neurosci", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14973250/" }, { "id": "PMID:11068039", "title": "Functional characterization of the human high-affinity choline transporter", "journal": "FEBS Lett", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11068039/" }, { "id": "PMID:15715662", "title": "Molecular characterization of the family of choline transporter-like proteins and their splice variants", "journal": "J Neurochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15715662/" }, { "id": "PMID:10677542", "title": "An electric lobe suppressor for a yeast choline transport mutation belongs to a new family of transporter-like proteins", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10677542/" } ], "x": 2660, "y": 1663.554794520548, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-449872", "label": "acetaldehyde [cytosolic] =\u003e acetaldehyde [mitochondrial]", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-449872", "summary": "acetaldehyde [cytosolic] =\u003e acetaldehyde [mitochondrial] is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CH3CHO to CH3CHO in cytosol · mitochondrial matrix.", "equation": "CH3CHO → CH3CHO", "inputs": [ { "node_id": "R-ALL-29510", "reactome_id": "R-ALL-29510", "label": "CH3CHO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113532", "reactome_id": "R-ALL-113532", "label": "CH3CHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:17567461", "title": "Modulation of mitochondrial membrane permeability in pathogenesis, autophagy and control of metabolism", "journal": "J Gastroenterol Hepatol", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17567461/" } ], "x": 8018.666666666667, "y": 3180.5588235294117, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-449911", "label": "acetate + CoA + ATP =\u003e acetyl-CoA + AMP + pyrophosphate [mitochondrial]", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:23177" ], "source_url": "https://reactome.org/content/detail/R-HSA-449911", "summary": "acetate + CoA + ATP =\u003e acetyl-CoA + AMP + pyrophosphate [mitochondrial] is a Reactome release 97 curated reaction event. The source record connects ATP, CH3COO-, CoA-SH to Ac-CoA, AMP, PPi in mitochondrial matrix and cross-references RHEA:23177.", "equation": "ATP + CH3COO- + CoA-SH → Ac-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113539", "reactome_id": "R-ALL-113539", "label": "CH3COO-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA synthetase activity of ACSS1 [mitochondrial matrix]", "activity_id": "449908", "ec_numbers": [ "6.2.1.1" ], "accessions": [ "Q9NUB1" ] } ], "citations": [ { "id": "PMID:16788062", "title": "Reversible lysine acetylation controls the activity of the mitochondrial enzyme acetyl-CoA synthetase 2", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16788062/" } ], "x": 7461.333333333333, "y": 2844.950980392157, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-450971", "label": "NNT dimer transfers proton from NADPH to NAD+", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrion", "compartment_ids": [ "GO:0005739" ], "rhea_ids": [ "RHEA:47992" ], "source_url": "https://reactome.org/content/detail/R-HSA-450971", "summary": "NNT dimer transfers proton from NADPH to NAD+ is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects NAD+, NADPH, H+ to H+, NADH, NADP+ in mitochondrion and cross-references RHEA:47992.", "equation": "NAD+ + NADPH + H+ → H+ + NADH + NADP+", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-163953", "reactome_id": "R-ALL-163953", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NAD(P)+ transhydrogenase (Si-specific) activity of NNT dimer [mitochondrial inner membrane]", "activity_id": "450969", "ec_numbers": [ "1.6.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10673423", "title": "The high-resolution structure of the NADP(H)-binding component (dIII) of proton-translocating transhydrogenase from human heart mitochondria", "journal": "Structure", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10673423/" }, { "id": "PMID:2005110", "title": "Mitochondrial energy-linked nicotinamide nucleotide transhydrogenase. Membrane topography of the bovine enzyme", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2005110/" }, { "id": "PMID:34155914", "title": "Mitochondrial NAD(P) + Transhydrogenase: From Molecular Features to Physiology and Disease", "journal": "Antioxid Redox Signal", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/34155914/" }, { "id": "PMID:8616157", "title": "The cDNA sequence of proton-pumping nicotinamide nucleotide transhydrogenase from man and mouse", "journal": "Biochim Biophys Acta", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8616157/" }, { "id": "PMID:31462775", "title": "Structure and mechanism of mitochondrial proton-translocating transhydrogenase", "journal": "Nature", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31462775/" } ], "x": 5670, "y": 287.3125, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-450984", "label": "IDH2 dimer decarboxylates isocitrate", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:19630", "RHEA:19629" ], "source_url": "https://reactome.org/content/detail/R-HSA-450984", "summary": "IDH2 dimer decarboxylates isocitrate is a Reactome release 97 curated reaction event. The source record connects ISCIT, NADP+ to 2OG, CO2, NADPH in mitochondrial matrix and cross-references RHEA:19630, RHEA:19629.", "equation": "ISCIT + NADP+ → 2OG + CO2 + NADPH", "inputs": [ { "node_id": "R-ALL-29938", "reactome_id": "R-ALL-29938", "label": "ISCIT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15562", "formula": "C6H5O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "isocitrate dehydrogenase (NADP+) activity of IDH2 dimer [mitochondrial matrix]", "activity_id": "450976", "ec_numbers": [ "1.1.1.42" ], "accessions": [] } ], "citations": [ { "id": "PMID:12207025", "title": "Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12207025/" }, { "id": "PMID:18806796", "title": "Insights from retinitis pigmentosa into the roles of isocitrate dehydrogenases in the Krebs cycle", "journal": "Nat Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18806796/" }, { "id": "PMID:14832224", "title": "Di- and triphosphopyridine nucleotide isocitric dehydrogenases in yeast", "journal": "J Biol Chem", "year": 1951, "url": "https://pubmed.ncbi.nlm.nih.gov/14832224/" }, { "id": "PMID:18914071", "title": "Biosynthesis of tricarboxylic acids by carbon dioxide fixation; enzymatic mechanisms", "journal": "J Biol Chem", "year": 1948, "url": "https://pubmed.ncbi.nlm.nih.gov/18914071/" }, { "id": "PMID:11278619", "title": "Control of mitochondrial redox balance and cellular defense against oxidative damage by mitochondrial NADP+-dependent isocitrate dehydrogenase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11278619/" }, { "id": "PMID:20847235", "title": "IDH2 mutations in patients with D-2-hydroxyglutaric aciduria", "journal": "Science", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20847235/" } ], "x": 5948.666666666667, "y": 335.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-452036", "label": "arginine + H2O =\u003e ornithine + urea [ARG2]", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:20570" ], "source_url": "https://reactome.org/content/detail/R-HSA-452036", "summary": "arginine + H2O =\u003e ornithine + urea [ARG2] is a Reactome release 97 curated reaction event. The source record connects L-Arg, H2O to L-Orn, Urea in mitochondrial matrix and cross-references RHEA:20570.", "equation": "L-Arg + H2O → L-Orn + Urea", "inputs": [ { "node_id": "R-ALL-374064", "reactome_id": "R-ALL-374064", "label": "L-Arg", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32682", "formula": "C6H15N4O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113585", "reactome_id": "R-ALL-113585", "label": "L-Orn", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:46911", "formula": "C5H13N2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-374061", "reactome_id": "R-ALL-374061", "label": "Urea", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16199", "formula": "CH4N2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arginase activity of ARG2 trimer [mitochondrial matrix]", "activity_id": "452020", "ec_numbers": [ "3.5.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12859189", "title": "Human arginase II: crystal structure and physiological role in male and female sexual arousal", "journal": "Biochemistry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12859189/" }, { "id": "PMID:8898077", "title": "Molecular cloning of cDNA for nonhepatic mitochondrial arginase (arginase II) and comparison of its induction with nitric oxide synthase in a murine macrophage-like cell line", "journal": "FEBS Lett", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8898077/" }, { "id": "PMID:9686347", "title": "The human arginases and arginase deficiency", "journal": "J Inherit Metab Dis", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9686347/" } ], "x": 1530, "y": 3179.666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-453337", "label": "glycogen phosphorylase (PYGB) dimer b + 2 ATP =\u003e glycogen phosphorylase (PYGB) dimer a + 2 ADP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17990" ], "source_url": "https://reactome.org/content/detail/R-HSA-453337", "summary": "glycogen phosphorylase (PYGB) dimer b + 2 ATP =\u003e glycogen phosphorylase (PYGB) dimer a + 2 ADP is a Reactome release 97 curated reaction event. The source record connects PYGB dimer b form, ATP to PYGB dimer a form, ADP, H+ in cytosol and cross-references RHEA:17990.", "equation": "PYGB dimer b form + 2 ATP → PYGB dimer a form + 2 ADP + 2 H+", "inputs": [ { "node_id": "R-HSA-71863", "reactome_id": "R-HSA-71863", "label": "PYGB dimer b form", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-71869", "reactome_id": "R-HSA-71869", "label": "PYGB dimer a form", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphorylase kinase activity of phosphorylase kinase complex (PHKM) [cytosol]", "activity_id": "71521", "ec_numbers": [ "2.7.11.19" ], "accessions": [] } ], "citations": [ { "id": "PMID:2667896", "title": "The family of glycogen phosphorylases: structure and function", "journal": "Crit Rev Biochem Mol Biol", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2667896/" } ], "x": 1808.6666666666667, "y": 846.7307692307693, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-453338", "label": "PYGM b dimer:AMP complex =\u003e PYGM dimer, b form + 2 AMP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-453338", "summary": "PYGM b dimer:AMP complex =\u003e PYGM dimer, b form + 2 AMP is a Reactome release 97 curated reaction event. The source record connects PYGM b dimer:AMP to PYGM dimer, b form, AMP in cytosol.", "equation": "PYGM b dimer:AMP → PYGM dimer, b form + 2 AMP", "inputs": [ { "node_id": "R-HSA-453344", "reactome_id": "R-HSA-453344", "label": "PYGM b dimer:AMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-71538", "reactome_id": "R-HSA-71538", "label": "PYGM dimer, b form", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:10949035", "title": "Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core", "journal": "Mol Cell", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10949035/" } ], "x": 1530, "y": 1086.1153846153848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-453339", "label": "poly((1,4)-alpha-glucosyl) glycogenin-2 + n orthophosphate =\u003e glycogenin-2 + n D-glucose 1-phosphate [PYGL]", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:41733" ], "source_url": "https://reactome.org/content/detail/R-HSA-453339", "summary": "poly((1,4)-alpha-glucosyl) glycogenin-2 + n orthophosphate =\u003e glycogenin-2 + n D-glucose 1-phosphate [PYGL] is a Reactome release 97 curated reaction event. The source record connects poly((1,4)-alpha-glucosyl)GYG2 dimer, Pi to GYG2 dimer, G1P in cytosol and cross-references RHEA:41733.", "equation": "poly((1,4)-alpha-glucosyl)GYG2 dimer + Pi → GYG2 dimer + G1P", "inputs": [ { "node_id": "R-HSA-453216", "reactome_id": "R-HSA-453216", "label": "poly((1,4)-alpha-glucosyl)GYG2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-70214", "reactome_id": "R-HSA-70214", "label": "GYG2 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29548", "reactome_id": "R-ALL-29548", "label": "G1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58601", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycogen phosphorylase activity of PYGL dimer a form [cytosol]", "activity_id": "71587", "ec_numbers": [ "2.4.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10949035", "title": "Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core", "journal": "Mol Cell", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10949035/" }, { "id": "PMID:2667896", "title": "The family of glycogen phosphorylases: structure and function", "journal": "Crit Rev Biochem Mol Biol", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2667896/" } ], "x": 1530, "y": 906.5769230769231, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-453342", "label": "PYGM dimer, b form + 2 AMP =\u003e PYGM b dimer:AMP complex", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-453342", "summary": "PYGM dimer, b form + 2 AMP =\u003e PYGM b dimer:AMP complex is a Reactome release 97 curated reaction event. The source record connects PYGM dimer, b form, AMP to PYGM b dimer:AMP in cytosol.", "equation": "PYGM dimer, b form + 2 AMP → PYGM b dimer:AMP", "inputs": [ { "node_id": "R-HSA-71538", "reactome_id": "R-HSA-71538", "label": "PYGM dimer, b form", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-453344", "reactome_id": "R-HSA-453344", "label": "PYGM b dimer:AMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "AMP binding activity of PYGM dimer, b form [cytosol]", "activity_id": "9931448", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10949035", "title": "Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core", "journal": "Mol Cell", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10949035/" } ], "x": 1808.6666666666667, "y": 966.4230769230769, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-453346", "label": "PYGB b dimer:AMP complex =\u003e PGYB dimer, b form + 2 AMP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-453346", "summary": "PYGB b dimer:AMP complex =\u003e PGYB dimer, b form + 2 AMP is a Reactome release 97 curated reaction event. The source record connects PYGB b dimer:AMP to PYGB dimer b form, AMP in cytosol.", "equation": "PYGB b dimer:AMP → PYGB dimer b form + 2 AMP", "inputs": [ { "node_id": "R-HSA-453332", "reactome_id": "R-HSA-453332", "label": "PYGB b dimer:AMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-71863", "reactome_id": "R-HSA-71863", "label": "PYGB dimer b form", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [], "x": 1808.6666666666667, "y": 1086.1153846153848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-453356", "label": "PYGB dimer, b form + 2 AMP =\u003e PYGB b dimer:AMP complex", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-453356", "summary": "PYGB dimer, b form + 2 AMP =\u003e PYGB b dimer:AMP complex is a Reactome release 97 curated reaction event. The source record connects PYGB dimer b form, AMP to PYGB b dimer:AMP in cytosol.", "equation": "PYGB dimer b form + 2 AMP → PYGB b dimer:AMP", "inputs": [ { "node_id": "R-HSA-71863", "reactome_id": "R-HSA-71863", "label": "PYGB dimer b form", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-453332", "reactome_id": "R-HSA-453332", "label": "PYGB b dimer:AMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "AMP binding activity of PYGB dimer b form [cytosol]", "activity_id": "9931464", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10949035", "title": "Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core", "journal": "Mol Cell", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10949035/" }, { "id": "PMID:2667896", "title": "The family of glycogen phosphorylases: structure and function", "journal": "Crit Rev Biochem Mol Biol", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2667896/" } ], "x": 1251.3333333333333, "y": 996.3461538461538, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-453358", "label": "poly((1,4)-alpha-glycosyl) glycogenin-1 + n orthophosphate =\u003e glycogenin-1 + n D-glucose 1-phosphate [PYGM,PYGB]", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:41733" ], "source_url": "https://reactome.org/content/detail/R-HSA-453358", "summary": "poly((1,4)-alpha-glycosyl) glycogenin-1 + n orthophosphate =\u003e glycogenin-1 + n D-glucose 1-phosphate [PYGM,PYGB] is a Reactome release 97 curated reaction event. The source record connects poly((1,4)-alpha-glucosyl)GYG1 dimer, Pi to GYG1 dimer, G1P in cytosol and cross-references RHEA:41733.", "equation": "poly((1,4)-alpha-glucosyl)GYG1 dimer + Pi → GYG1 dimer + G1P", "inputs": [ { "node_id": "R-HSA-453217", "reactome_id": "R-HSA-453217", "label": "poly((1,4)-alpha-glucosyl)GYG1 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-70203", "reactome_id": "R-HSA-70203", "label": "GYG1 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29548", "reactome_id": "R-ALL-29548", "label": "G1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58601", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycogen phosphorylase activity of active PYGM and PYGB dimers [cytosol]", "activity_id": "71514", "ec_numbers": [ "2.4.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10949035", "title": "Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core", "journal": "Mol Cell", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10949035/" }, { "id": "PMID:2667896", "title": "The family of glycogen phosphorylases: structure and function", "journal": "Crit Rev Biochem Mol Biol", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2667896/" } ], "x": 1808.6666666666667, "y": 906.5769230769231, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-4546387", "label": "PIAS4 SUMOylates VDR with SUMO2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-4546387", "summary": "PIAS4 SUMOylates VDR with SUMO2 is a Reactome release 97 curated reaction event. The source record connects VDR, SUMO2:UBE2I to polySUMO2-VDR, UBE2I in nucleoplasm.", "equation": "VDR + 2 SUMO2:UBE2I → polySUMO2-VDR + 2 UBE2I", "inputs": [ { "node_id": "R-HSA-375512", "reactome_id": "R-HSA-375512", "label": "VDR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2993778", "reactome_id": "R-HSA-2993778", "label": "SUMO2:UBE2I", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-4546376", "reactome_id": "R-HSA-4546376", "label": "polySUMO2-VDR", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-912481", "reactome_id": "R-HSA-912481", "label": "UBE2I", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "SUMO transferase activity of PIAS4 [nucleoplasm]", "activity_id": "3215011", "ec_numbers": null, "accessions": [ "Q8N2W9" ] } ], "citations": [ { "id": "PMID:22564762", "title": "PIAS4 represses vitamin D receptor-mediated signaling and acts as an E3-SUMO ligase towards vitamin D receptor", "journal": "J. Steroid Biochem. Mol. Biol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22564762/" } ], "x": 4730, "y": 1807.0681818181818, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-469659", "label": "SRD5A1 dehydrogenates TEST to DHTEST", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:50822" ], "source_url": "https://reactome.org/content/detail/R-HSA-469659", "summary": "SRD5A1 dehydrogenates TEST to DHTEST is a Reactome release 97 curated reaction event. The source record connects TEST, NADPH, H+ to DHTEST, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:50822.", "equation": "TEST + NADPH + H+ → DHTEST + NADP+", "inputs": [ { "node_id": "R-ALL-193057", "reactome_id": "R-ALL-193057", "label": "TEST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17347", "formula": "C19H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-469662", "reactome_id": "R-ALL-469662", "label": "DHTEST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16330", "formula": "C19H30O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-oxo-5-alpha-steroid 4-dehydrogenase activity of SRD5A1 [endoplasmic reticulum membrane]", "activity_id": "469661", "ec_numbers": [ "1.3.99.5" ], "accessions": [ "P18405" ] } ], "citations": [ { "id": "PMID:2339109", "title": "Structural and biochemical properties of cloned and expressed human and rat steroid 5 alpha-reductases", "journal": "Proc Natl Acad Sci U S A", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2339109/" }, { "id": "PMID:9536209", "title": "A critical review of the origin and control of adrenal androgens", "journal": "Baillieres Clin Obstet Gynaecol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9536209/" } ], "x": 5008.666666666667, "y": 1606.6742424242425, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-482619", "label": "(d)NDP + ATP (d)NTP + ADP (NME1,2,3)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27687", "RHEA:28583", "RHEA:25238", "RHEA:25099", "RHEA:27675", "RHEA:27691", "RHEA:27679", "RHEA:27683" ], "source_url": "https://reactome.org/content/detail/R-HSA-482619", "summary": "(d)NDP + ATP (d)NTP + ADP (NME1,2,3) is a Reactome release 97 curated reaction event. The source record connects (d)NDP, ATP to (d)NTP, ADP in cytosol and cross-references RHEA:27687, RHEA:28583, RHEA:25238, RHEA:25099, RHEA:27675, RHEA:27691, RHEA:27679, RHEA:27683.", "equation": "(d)NDP + ATP → (d)NTP + ADP", "inputs": [ { "node_id": "R-ALL-482627", "reactome_id": "R-ALL-482627", "label": "(d)NDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-482625", "reactome_id": "R-ALL-482625", "label": "(d)NTP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate kinase activity of NME1,2 hexamers [cytosol]", "activity_id": "482613", "ec_numbers": [ "2.7.4.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:13211603", "title": "Enzymatic phosphorylation of nucleoside diphosphates", "journal": "J Biol Chem", "year": 1954, "url": "https://pubmed.ncbi.nlm.nih.gov/13211603/" }, { "id": "PMID:12972261", "title": "Nucleotide binding to nucleoside diphosphate kinases: X-ray structure of human NDPK-A in complex with ADP and comparison to protein kinases", "journal": "J Mol Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12972261/" }, { "id": "PMID:11277919", "title": "Structural and catalytic properties and homology modelling of the human nucleoside diphosphate kinase C, product of the DRnm23 gene", "journal": "Eur J Biochem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11277919/" }, { "id": "PMID:1851158", "title": "Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1851158/" }, { "id": "PMID:9488696", "title": "Substrate specificity of human nucleoside-diphosphate kinase revealed by transient kinetic analysis", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9488696/" } ], "x": 3321.3333333333335, "y": 3048.7291666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-482621", "label": "(d)NTP + ADP (d)NDP + ATP (NME1,2,3)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27688", "RHEA:28584", "RHEA:25239", "RHEA:25100", "RHEA:27676", "RHEA:27692", "RHEA:27680", "RHEA:27684" ], "source_url": "https://reactome.org/content/detail/R-HSA-482621", "summary": "(d)NTP + ADP (d)NDP + ATP (NME1,2,3) is a Reactome release 97 curated reaction event. The source record connects (d)NTP, ADP to (d)NDP, ATP in cytosol and cross-references RHEA:27688, RHEA:28584, RHEA:25239, RHEA:25100, RHEA:27676, RHEA:27692, RHEA:27680, RHEA:27684.", "equation": "(d)NTP + ADP → (d)NDP + ATP", "inputs": [ { "node_id": "R-ALL-482625", "reactome_id": "R-ALL-482625", "label": "(d)NTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-482627", "reactome_id": "R-ALL-482627", "label": "(d)NDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate kinase activity of NME1,2 hexamers [cytosol]", "activity_id": "482613", "ec_numbers": [ "2.7.4.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:13211603", "title": "Enzymatic phosphorylation of nucleoside diphosphates", "journal": "J Biol Chem", "year": 1954, "url": "https://pubmed.ncbi.nlm.nih.gov/13211603/" }, { "id": "PMID:12972261", "title": "Nucleotide binding to nucleoside diphosphate kinases: X-ray structure of human NDPK-A in complex with ADP and comparison to protein kinases", "journal": "J Mol Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12972261/" }, { "id": "PMID:11277919", "title": "Structural and catalytic properties and homology modelling of the human nucleoside diphosphate kinase C, product of the DRnm23 gene", "journal": "Eur J Biochem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11277919/" }, { "id": "PMID:1851158", "title": "Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1851158/" }, { "id": "PMID:9488696", "title": "Substrate specificity of human nucleoside-diphosphate kinase revealed by transient kinetic analysis", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9488696/" } ], "x": 3600, "y": 3048.7291666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-482804", "label": "(d)NDP + ATP (d)NTP + ADP (NME4)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:27675", "RHEA:27687", "RHEA:25238", "RHEA:25099", "RHEA:27691", "RHEA:27679", "RHEA:27683", "RHEA:28583" ], "source_url": "https://reactome.org/content/detail/R-HSA-482804", "summary": "(d)NDP + ATP (d)NTP + ADP (NME4) is a Reactome release 97 curated reaction event. The source record connects (d)NDP, ATP to (d)NTP, ADP in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:27675, RHEA:27687, RHEA:25238, RHEA:25099, RHEA:27691, RHEA:27679, RHEA:27683, RHEA:28583.", "equation": "(d)NDP + ATP → (d)NTP + ADP", "inputs": [ { "node_id": "R-ALL-482803", "reactome_id": "R-ALL-482803", "label": "(d)NDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-482807", "reactome_id": "R-ALL-482807", "label": "(d)NTP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate kinase activity of NME4 hexamer [mitochondrial inner membrane]", "activity_id": "110637", "ec_numbers": [ "2.7.4.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:10799505", "title": "The human nm23-H4 gene product is a mitochondrial nucleoside diphosphate kinase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10799505/" }, { "id": "PMID:9488696", "title": "Substrate specificity of human nucleoside-diphosphate kinase revealed by transient kinetic analysis", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9488696/" }, { "id": "PMID:18635542", "title": "The nucleoside diphosphate kinase D (NM23-H4) binds the inner mitochondrial membrane with high affinity to cardiolipin and couples nucleotide transfer with respiration", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18635542/" } ], "x": 3878.666666666667, "y": 3048.7291666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-482812", "label": "(d)NTP + ADP (d)NDP + ATP (NME4)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:27676", "RHEA:27688", "RHEA:25239", "RHEA:25100", "RHEA:27692", "RHEA:27680", "RHEA:27684", "RHEA:28584" ], "source_url": "https://reactome.org/content/detail/R-HSA-482812", "summary": "(d)NTP + ADP (d)NDP + ATP (NME4) is a Reactome release 97 curated reaction event. The source record connects (d)NTP, ADP to (d)NDP, ATP in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:27676, RHEA:27688, RHEA:25239, RHEA:25100, RHEA:27692, RHEA:27680, RHEA:27684, RHEA:28584.", "equation": "(d)NTP + ADP → (d)NDP + ATP", "inputs": [ { "node_id": "R-ALL-482807", "reactome_id": "R-ALL-482807", "label": "(d)NTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-482803", "reactome_id": "R-ALL-482803", "label": "(d)NDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate kinase activity of NME4 hexamer [mitochondrial inner membrane]", "activity_id": "110637", "ec_numbers": [ "2.7.4.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:10799505", "title": "The human nm23-H4 gene product is a mitochondrial nucleoside diphosphate kinase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10799505/" }, { "id": "PMID:9488696", "title": "Substrate specificity of human nucleoside-diphosphate kinase revealed by transient kinetic analysis", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9488696/" }, { "id": "PMID:18635542", "title": "The nucleoside diphosphate kinase D (NM23-H4) binds the inner mitochondrial membrane with high affinity to cardiolipin and couples nucleotide transfer with respiration", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18635542/" } ], "x": 3321.3333333333335, "y": 3061.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-504054", "label": "Amination of uridine 5'-triphosphate (UTP) to form cytidine 5'-triphosphate (CTPS2)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:26427" ], "source_url": "https://reactome.org/content/detail/R-HSA-504054", "summary": "Amination of uridine 5'-triphosphate (UTP) to form cytidine 5'-triphosphate (CTPS2) is a Reactome release 97 curated reaction event. The source record connects UTP, ATP, L-Gln to CTP, ADP, L-Glu in cytosol and cross-references RHEA:26427.", "equation": "UTP + ATP + L-Gln + H2O → CTP + ADP + L-Glu + Pi + 2 H+", "inputs": [ { "node_id": "R-ALL-29494", "reactome_id": "R-ALL-29494", "label": "UTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46398", "formula": "C9H11N2O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-110614", "reactome_id": "R-ALL-110614", "label": "CTP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:37563", "formula": "C9H12N3O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "CTP synthase activity of CTPS2 tetramer [cytosol]", "activity_id": "504051", "ec_numbers": [ "6.3.4.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:16179339", "title": "Expression of Human CTP synthetase in Saccharomyces cerevisiae reveals phosphorylation by protein kinase A", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16179339/" }, { "id": "PMID:10899599", "title": "Identification of a cDNA encoding an isoform of human CTP synthetase", "journal": "Biochim Biophys Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10899599/" } ], "x": 3321.3333333333335, "y": 3111.0208333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-508040", "label": "glutamate + ATP + NADPH + H+ =\u003e L-glutamate gamma-semialdehyde + NADP+ + ADP + orthophosphate [P5CS]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:33208" ], "source_url": "https://reactome.org/content/detail/R-HSA-508040", "summary": "glutamate + ATP + NADPH + H+ =\u003e L-glutamate gamma-semialdehyde + NADP+ + ADP + orthophosphate [P5CS] is a Reactome release 97 curated reaction event. The source record connects L-Glu, ATP, NADPH to L-Glu5S, NADP+, ADP in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:33208.", "equation": "L-Glu + ATP + NADPH + H+ → L-Glu5S + NADP+ + ADP + Pi", "inputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31339", "reactome_id": "R-ALL-31339", "label": "L-Glu5S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58066", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "delta1-pyrroline-5-carboxylate synthetase activity of P5CS dimers [mitochondrial inner membrane]", "activity_id": "508035", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10037775", "title": "Molecular enzymology of mammalian Delta1-pyrroline-5-carboxylate synthase. Alternative splice donor utilization generates isoforms with different sensitivity to ornithine inhibition", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10037775/" } ], "x": 7461.333333333333, "y": 1547.95, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-508179", "label": "a-ketoisocaproate, a-keto-b-methylvalerate, or a-ketoisovalerate + glutamate leu, ile, or val + alpha-ketoglutarate [BCAT2]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24815", "RHEA:18323", "RHEA:24803" ], "source_url": "https://reactome.org/content/detail/R-HSA-508179", "summary": "a-ketoisocaproate, a-keto-b-methylvalerate, or a-ketoisovalerate + glutamate leu, ile, or val + alpha-ketoglutarate [BCAT2] is a Reactome release 97 curated reaction event. The source record connects KIC, KMVA, KIV, L-Glu to 2OG, Leu, Ile, Val in mitochondrial matrix and cross-references RHEA:24815, RHEA:18323, RHEA:24803.", "equation": "KIC, KMVA, KIV + L-Glu → 2OG + Leu, Ile, Val", "inputs": [ { "node_id": "R-ALL-508181", "reactome_id": "R-ALL-508181", "label": "KIC, KMVA, KIV", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-508190", "reactome_id": "R-ALL-508190", "label": "Leu, Ile, Val", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "branched-chain-amino-acid:2-oxoglutarate transaminase activity of BCAT2 dimer [mitochondrial matrix]", "activity_id": "70711", "ec_numbers": [ "2.6.1.42" ], "accessions": [] } ], "citations": [ { "id": "PMID:9165094", "title": "Cloning of the rat and human mitochondrial branched chain aminotransferases (BCATm)", "journal": "Biochim Biophys Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9165094/" }, { "id": "PMID:11264579", "title": "The structure of human mitochondrial branched-chain aminotransferase.", "journal": "Acta Crystallogr D Biol Crystallogr", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11264579/" }, { "id": "PMID:12269802", "title": "Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: ketimine and pyridoxamine phosphate forms", "journal": "Biochemistry", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12269802/" } ], "x": 6800, "y": 1541.3055555555557, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-508189", "label": "a-ketoisocaproate, a-keto-b-methylvalerate, or a-ketoisovalerate + glutamate leu, ile, or val + alpha-ketoglutarate [BCAT1]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18323", "RHEA:24803", "RHEA:24815" ], "source_url": "https://reactome.org/content/detail/R-HSA-508189", "summary": "a-ketoisocaproate, a-keto-b-methylvalerate, or a-ketoisovalerate + glutamate leu, ile, or val + alpha-ketoglutarate [BCAT1] is a Reactome release 97 curated reaction event. The source record connects KIC,KMVA,KIV, L-Glu to Leu, Ile, Val, 2OG in cytosol and cross-references RHEA:18323, RHEA:24803, RHEA:24815.", "equation": "KIC,KMVA,KIV + L-Glu → Leu, Ile, Val + 2OG", "inputs": [ { "node_id": "R-ALL-508187", "reactome_id": "R-ALL-508187", "label": "KIC,KMVA,KIV", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-508182", "reactome_id": "R-ALL-508182", "label": "Leu, Ile, Val", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "branched-chain-amino-acid:2-oxoglutarate transaminase activity of BCAT1 dimer [cytosol]", "activity_id": "70700", "ec_numbers": [ "2.6.1.42" ], "accessions": [] } ], "citations": [ { "id": "PMID:16141215", "title": "Structural determinants for branched-chain aminotransferase isozyme-specific inhibition by the anticonvulsant drug gabapentin", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16141215/" } ], "x": 6521.333333333333, "y": 1557.9166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-508308", "label": "beta-methylglutaconyl-CoA + ADP + orthophosphate beta-methylcrotonyl-CoA + ATP + HCO3- (MCCA)", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:13591" ], "source_url": "https://reactome.org/content/detail/R-HSA-508308", "summary": "beta-methylglutaconyl-CoA + ADP + orthophosphate beta-methylcrotonyl-CoA + ATP + HCO3- (MCCA) is a Reactome release 97 curated reaction event. The source record connects bMG-CoA, ADP, Pi to bMC-CoA, ATP, HCO3- in mitochondrial matrix and cross-references RHEA:13591.", "equation": "bMG-CoA + ADP + Pi + H+ → bMC-CoA + ATP + HCO3-", "inputs": [ { "node_id": "R-ALL-70772", "reactome_id": "R-ALL-70772", "label": "bMG-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57346", "formula": "C27H37N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70744", "reactome_id": "R-ALL-70744", "label": "bMC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57344", "formula": "C26H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29896", "reactome_id": "R-ALL-29896", "label": "HCO3-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methylcrotonoyl-CoA carboxylase activity of 6x(Btn-MCCC1:MCCC2) [mitochondrial matrix]", "activity_id": "70771", "ec_numbers": [ "6.4.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:11181649", "title": "The molecular basis of human 3-methylcrotonyl-CoA carboxylase deficiency.", "journal": "J Clin Invest", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11181649/" }, { "id": "PMID:11406611", "title": "Cloning of the human MCCA and MCCB genes and mutations therein reveal the molecular cause of 3-methylcrotonyl-CoA: carboxylase deficiency.", "journal": "Hum Mol Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11406611/" } ], "x": 6521.333333333333, "y": 1574.5277777777778, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-508369", "label": "alpha-methylacetoacetyl-CoA + NADH + H+ alpha-methyl-beta-hydroxybutyryl-CoA + NAD+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:13283" ], "source_url": "https://reactome.org/content/detail/R-HSA-508369", "summary": "alpha-methylacetoacetyl-CoA + NADH + H+ alpha-methyl-beta-hydroxybutyryl-CoA + NAD+ is a Reactome release 97 curated reaction event. The source record connects 2MACA-CoA, NADH, H+ to aMbHBUT-CoA, NAD+ in mitochondrial matrix and cross-references RHEA:13283.", "equation": "2MACA-CoA + NADH + H+ → aMbHBUT-CoA + NAD+", "inputs": [ { "node_id": "R-ALL-70836", "reactome_id": "R-ALL-70836", "label": "2MACA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57335", "formula": "C26H38N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70829", "reactome_id": "R-ALL-70829", "label": "aMbHBUT-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57312", "formula": "C26H40N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of HSD17B10 tetramer [mitochondrial matrix]", "activity_id": "70835", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:15342248", "title": "Crystal structure of human ABAD/HSD10 with a bound inhibitor: implications for design of Alzheimer's disease therapeutics", "journal": "J Mol Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15342248/" }, { "id": "PMID:12696021", "title": "2-Methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency is caused by mutations in the HADH2 gene.", "journal": "Am J Hum Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12696021/" } ], "x": 7078.666666666667, "y": 1574.5277777777778, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-508473", "label": "methylmalonyl semialdehyde + NADH + H+ beta-hydroxyisobutyrate + NAD+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31277" ], "source_url": "https://reactome.org/content/detail/R-HSA-508473", "summary": "methylmalonyl semialdehyde + NADH + H+ beta-hydroxyisobutyrate + NAD+ is a Reactome release 97 curated reaction event. The source record connects 2M3OPROA, NADH, H+ to NAD+, bHIBA in mitochondrial matrix and cross-references RHEA:31277.", "equation": "2M3OPROA + NADH + H+ → NAD+ + bHIBA", "inputs": [ { "node_id": "R-ALL-30004", "reactome_id": "R-ALL-30004", "label": "2M3OPROA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62413", "formula": "C4H5O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-573404", "reactome_id": "R-ALL-573404", "label": "bHIBA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62638", "formula": "C4H7O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-hydroxyisobutyrate dehydrogenase activity of HIBADH tetramer [mitochondrial matrix]", "activity_id": "70884", "ec_numbers": [ "1.1.1.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:13416257", "title": "Purification and properties of beta-hydroxyisobutyric dehydrogenase", "journal": "J Biol Chem", "year": 1957, "url": "https://pubmed.ncbi.nlm.nih.gov/13416257/" } ], "x": 6521.333333333333, "y": 1591.138888888889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-508561", "label": "alpha-ketoadipate + glutamate alpha-aminoadipate + alpha-ketoglutarate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12603" ], "source_url": "https://reactome.org/content/detail/R-HSA-508561", "summary": "alpha-ketoadipate + glutamate alpha-aminoadipate + alpha-ketoglutarate is a Reactome release 97 curated reaction event. The source record connects L-Glu, 2-OA to 2OG, 2AMA in mitochondrial matrix and cross-references RHEA:12603.", "equation": "L-Glu + 2-OA → 2OG + 2AMA", "inputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70951", "reactome_id": "R-ALL-70951", "label": "2-OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57499", "formula": "C6H6O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-508531", "reactome_id": "R-ALL-508531", "label": "2AMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58672", "formula": "C6H10NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-2-aminoadipate:2-oxoglutarate transaminase activity of AADAT dimer [mitochondrial matrix]", "activity_id": "70950", "ec_numbers": [ "2.6.1.39" ], "accessions": [] } ], "citations": [ { "id": "PMID:12126930", "title": "Characterization of the human gene encoding alpha-aminoadipate aminotransferase (AADAT).", "journal": "Mol Genet Metab", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12126930/" }, { "id": "PMID:18056995", "title": "Crystal structure of human kynurenine aminotransferase II", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18056995/" }, { "id": "PMID:18056996", "title": "Crystal structure of human kynurenine aminotransferase II, a drug target for the treatment of schizophrenia", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18056996/" } ], "x": 6521.333333333333, "y": 2099.2083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-514604", "label": "GMP + NADPH + 2 H+ =\u003e IMP + NADP+ + NH4+ (GMPR,GMPR2)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17187" ], "source_url": "https://reactome.org/content/detail/R-HSA-514604", "summary": "GMP + NADPH + 2 H+ =\u003e IMP + NADP+ + NH4+ (GMPR,GMPR2) is a Reactome release 97 curated reaction event. The source record connects GMP, NADPH, H+ to NADP+, NH4+, IMP in cytosol and cross-references RHEA:17187.", "equation": "GMP + NADPH + 2 H+ → NADP+ + NH4+ + IMP", "inputs": [ { "node_id": "R-ALL-113578", "reactome_id": "R-ALL-113578", "label": "GMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58115", "formula": "C10H12N5O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29602", "reactome_id": "R-ALL-29602", "label": "IMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58053", "formula": "C10H11N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "GMP reductase activity of GMPR tetramers [cytosol]", "activity_id": "514632", "ec_numbers": [ "1.7.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:12009299", "title": "NADPH-dependent GMP reductase isoenzyme of human (GMPR2). Expression, purification, and kinetic properties", "journal": "Int J Biochem Cell Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12009299/" }, { "id": "PMID:16359702", "title": "Crystal structure of human guanosine monophosphate reductase 2 (GMPR2) in complex with GMP", "journal": "J Mol Biol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16359702/" }, { "id": "PMID:218932", "title": "Reaction mechanism and specificity of human GMP reductase. Substrates, inhibitors, activators, and inactivators", "journal": "J Biol Chem", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/218932/" } ], "x": 3878.666666666667, "y": 2835.035714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-517444", "label": "TAT aminates HPP", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15095" ], "source_url": "https://reactome.org/content/detail/R-HSA-517444", "summary": "TAT aminates HPP is a Reactome release 97 curated reaction event. The source record connects HPP, L-Glu to L-Tyr, 2OG in cytosol and cross-references RHEA:15095.", "equation": "HPP + L-Glu → L-Tyr + 2OG", "inputs": [ { "node_id": "R-ALL-31365", "reactome_id": "R-ALL-31365", "label": "HPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36242", "formula": "C9H7O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29506", "reactome_id": "R-ALL-29506", "label": "L-Tyr", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58315", "formula": "C9H11NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-tyrosine:2-oxoglutarate transaminase activity of TAT dimer [cytosol]", "activity_id": "71154", "ec_numbers": [ "2.6.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:21153519", "title": "Tyrosine aminotransferase: biochemical and structural properties and molecular dynamics simulations", "journal": "Protein Cell", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21153519/" }, { "id": "PMID:16640556", "title": "The narrow substrate specificity of human tyrosine aminotransferase--the enzyme deficient in tyrosinemia type II", "journal": "FEBS J", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16640556/" }, { "id": "PMID:16602095", "title": "The genetic tyrosinemias", "journal": "Am J Med Genet C Semin Med Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16602095/" } ], "x": 6800, "y": 1817.35, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5223313", "label": "ABCD4:LMBRD1 transports RCbl from lysosomal lumen to cytosol (gut mucosal cells)", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · lysosomal lumen · lysosomal membrane", "compartment_ids": [ "GO:0005829", "GO:0043202", "GO:0005765" ], "rhea_ids": [ "RHEA:17873" ], "source_url": "https://reactome.org/content/detail/R-HSA-5223313", "summary": "ABCD4:LMBRD1 transports RCbl from lysosomal lumen to cytosol (gut mucosal cells) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATP, H2O, RCbl to ADP, Pi, RCbl in cytosol · lysosomal lumen · lysosomal membrane and cross-references RHEA:17873.", "equation": "ATP + H2O + RCbl → ADP + Pi + RCbl", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9759390", "reactome_id": "R-ALL-9759390", "label": "RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9759394", "reactome_id": "R-ALL-9759394", "label": "RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ABC-type vitamin B12 transporter activity of ABCD4:LMBRD1 [lysosomal membrane]", "activity_id": "5223338", "ec_numbers": [ "7.6.2.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:22922874", "title": "Mutations in ABCD4 cause a new inborn error of vitamin B12 metabolism", "journal": "Nat. Genet.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22922874/" }, { "id": "PMID:27456980", "title": "Translocation of the ABC transporter ABCD4 from the endoplasmic reticulum to lysosomes requires the escort protein LMBD1", "journal": "Sci Rep", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27456980/" }, { "id": "PMID:33845046", "title": "The lysosomal protein ABCD4 can transport vitamin B 12 across liposomal membranes in vitro", "journal": "J Biol Chem", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33845046/" }, { "id": "PMID:19136951", "title": "Identification of a putative lysosomal cobalamin exporter altered in the cblF defect of vitamin B12 metabolism", "journal": "Nat. Genet.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19136951/" }, { "id": "PMID:4001945", "title": "Defect in vitamin B12 release from lysosomes: newly described inborn error of vitamin B12 metabolism", "journal": "Science", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/4001945/" } ], "x": 5948.666666666667, "y": 2890.0131578947367, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-5250531", "label": "ARV1 transports CHOL from ER membrane to plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane · plasma membrane", "compartment_ids": [ "GO:0005788", "GO:0005789", "GO:0005886" ], "rhea_ids": [ "RHEA:39747" ], "source_url": "https://reactome.org/content/detail/R-HSA-5250531", "summary": "ARV1 transports CHOL from ER membrane to plasma membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CHOL to CHOL in endoplasmic reticulum lumen · endoplasmic reticulum membrane · plasma membrane and cross-references RHEA:39747.", "equation": "CHOL → CHOL", "inputs": [ { "node_id": "R-ALL-193384", "reactome_id": "R-ALL-193384", "label": "CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-171095", "reactome_id": "R-ALL-171095", "label": "CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sterol transfer activity of ARV1 [endoplasmic reticulum membrane]", "activity_id": "5250542", "ec_numbers": null, "accessions": [ "Q9H2C2" ] } ], "citations": [ { "id": "PMID:12145310", "title": "Yeast cells lacking the ARV1 gene harbor defects in sphingolipid metabolism. Complementation by human ARV1", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12145310/" }, { "id": "PMID:11063737", "title": "Mutations in yeast ARV1 alter intracellular sterol distribution and are complemented by human ARV1", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11063737/" } ], "x": 2381.3333333333335, "y": 2284.3, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-5333678", "label": "CPNEs bind PL", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5333678", "summary": "CPNEs bind PL is a Reactome release 97 curated reaction event. The source record connects CPNEs, PL to CPNEs:PL in plasma membrane.", "equation": "CPNEs + PL → CPNEs:PL", "inputs": [ { "node_id": "R-HSA-5333699", "reactome_id": "R-HSA-5333699", "label": "CPNEs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-171142", "reactome_id": "R-ALL-171142", "label": "PL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16247", "formula": "C5H6O8PR3", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5333679", "reactome_id": "R-HSA-5333679", "label": "CPNEs:PL", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein-membrane adaptor activity of CPNEs [plasma membrane]", "activity_id": "9949549", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12440769", "title": "Copines: a ubiquitous family of Ca(2+)-dependent phospholipid-binding proteins", "journal": "Cell. Mol. Life Sci.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12440769/" }, { "id": "PMID:9430674", "title": "The copines, a novel class of C2 domain-containing, calcium-dependent, phospholipid-binding proteins conserved from Paramecium to humans", "journal": "J. Biol. Chem.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9430674/" } ], "x": 2938.666666666667, "y": 1860.7191780821918, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5334794", "label": "FABPs bind LCFAs", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5334794", "summary": "FABPs bind LCFAs is a Reactome release 97 curated reaction event. The source record connects FABPs, LCFA(-) to FABPs:LCFA in cytosol.", "equation": "FABPs + LCFA(-) → FABPs:LCFA", "inputs": [ { "node_id": "R-HSA-5334833", "reactome_id": "R-HSA-5334833", "label": "FABPs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-8876730", "reactome_id": "R-ALL-8876730", "label": "LCFA(-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5334829", "reactome_id": "R-HSA-5334829", "label": "FABPs:LCFA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "lipid carrier activity of FABPs [cytosol]", "activity_id": "9958498", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:21504868", "title": "The human fatty acid-binding protein family: evolutionary divergences and functions", "journal": "Hum. Genomics", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21504868/" } ], "x": 3878.666666666667, "y": 2279.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5340195", "label": "NR1H4 binds DCA, CDCA, LCHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5340195", "summary": "NR1H4 binds DCA, CDCA, LCHA is a Reactome release 97 curated reaction event. The source record connects DCA,CDCA,LCHA, NR1H4 to NR1H4:DCA,CDCA,LCHA in nucleoplasm.", "equation": "DCA,CDCA,LCHA + NR1H4 → NR1H4:DCA,CDCA,LCHA", "inputs": [ { "node_id": "R-ALL-5340249", "reactome_id": "R-ALL-5340249", "label": "DCA,CDCA,LCHA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-5340228", "reactome_id": "R-HSA-5340228", "label": "NR1H4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5340186", "reactome_id": "R-HSA-5340186", "label": "NR1H4:DCA,CDCA,LCHA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "bile acid nuclear receptor activity of NR1H4 [nucleoplasm]", "activity_id": "9917215", "ec_numbers": null, "accessions": [ "Q96RI1" ] } ], "citations": [ { "id": "PMID:10334993", "title": "Bile acids: natural ligands for an orphan nuclear receptor", "journal": "Science", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10334993/" }, { "id": "PMID:10334992", "title": "Identification of a nuclear receptor for bile acids", "journal": "Science", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10334992/" }, { "id": "PMID:12393883", "title": "Natural structural variants of the nuclear receptor farnesoid X receptor affect transcriptional activation", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12393883/" }, { "id": "PMID:12815072", "title": "Definition of a novel growth factor-dependent signal cascade for the suppression of bile acid biosynthesis", "journal": "Genes Dev.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12815072/" }, { "id": "PMID:16541101", "title": "Endocrine functions of bile acids", "journal": "EMBO J.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16541101/" }, { "id": "PMID:16908160", "title": "FXR, a multipurpose nuclear receptor", "journal": "Trends Biochem. Sci.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16908160/" } ], "x": 5008.666666666667, "y": 1807.0681818181818, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5340251", "label": "NR1H4:DCA,CDCA,LCHA binds RXRA and NCOA1,2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5340251", "summary": "NR1H4:DCA,CDCA,LCHA binds RXRA and NCOA1,2 is a Reactome release 97 curated reaction event. The source record connects NR1H4:DCA,CDCA,LCHA, NCOA1,NCOA2,RXRA to NCOA1,2:RXRA:NR1H4:DCA,CDCA,LCHA in nucleoplasm.", "equation": "NR1H4:DCA,CDCA,LCHA + NCOA1,NCOA2,RXRA → NCOA1,2:RXRA:NR1H4:DCA,CDCA,LCHA", "inputs": [ { "node_id": "R-HSA-5340186", "reactome_id": "R-HSA-5340186", "label": "NR1H4:DCA,CDCA,LCHA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9957259", "reactome_id": "R-HSA-9957259", "label": "NCOA1,NCOA2,RXRA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5340274", "reactome_id": "R-HSA-5340274", "label": "NCOA1,2:RXRA:NR1H4:DCA,CDCA,LCHA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "transcription coregulator activity of NCOA1,NCOA2,RXRA [nucleoplasm]", "activity_id": "9957262", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10334993", "title": "Bile acids: natural ligands for an orphan nuclear receptor", "journal": "Science", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10334993/" }, { "id": "PMID:12815072", "title": "Definition of a novel growth factor-dependent signal cascade for the suppression of bile acid biosynthesis", "journal": "Genes Dev.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12815072/" }, { "id": "PMID:16541101", "title": "Endocrine functions of bile acids", "journal": "EMBO J.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16541101/" }, { "id": "PMID:11870371", "title": "Farnesoid X receptor and bile salts are involved in transcriptional regulation of the gene encoding the human bile salt export pump", "journal": "Hepatology", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11870371/" }, { "id": "PMID:11387316", "title": "Human bile salt export pump promoter is transactivated by the farnesoid X receptor/bile acid receptor", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11387316/" }, { "id": "PMID:10334992", "title": "Identification of a nuclear receptor for bile acids", "journal": "Science", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10334992/" } ], "x": 4451.333333333333, "y": 1812.9621212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5359451", "label": "NNMT transfers CH3 from SAM to NAM to form MNA", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23885" ], "source_url": "https://reactome.org/content/detail/R-HSA-5359451", "summary": "NNMT transfers CH3 from SAM to NAM to form MNA is a Reactome release 97 curated reaction event. The source record connects NAM, AdoMet to AdoHcy, MNA in cytosol and cross-references RHEA:23885.", "equation": "NAM + AdoMet → AdoHcy + MNA", "inputs": [ { "node_id": "R-ALL-197277", "reactome_id": "R-ALL-197277", "label": "NAM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-549282", "reactome_id": "R-ALL-549282", "label": "MNA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16797", "formula": "C7H9N2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinamide N-methyltransferase activity of NNMT [cytosol]", "activity_id": "159343", "ec_numbers": [ "2.1.1.1" ], "accessions": [ "P40261" ] } ], "citations": [ { "id": "PMID:8182091", "title": "Human liver nicotinamide N-methyltransferase. cDNA cloning, expression, and biochemical characterization", "journal": "J. Biol. Chem.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8182091/" }, { "id": "PMID:8575745", "title": "Human nicotinamide N-methyltransferase gene: molecular cloning, structural characterization and chromosomal localization", "journal": "Genomics", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8575745/" }, { "id": "PMID:24717514", "title": "Nicotinamide N-methyltransferase knockdown protects against diet-induced obesity", "journal": "Nature", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24717514/" }, { "id": "PMID:17641676", "title": "1-Methylnicotinamide (MNA), a primary metabolite of nicotinamide, exerts anti-thrombotic activity mediated by a cyclooxygenase-2/prostacyclin pathway", "journal": "Br. J. Pharmacol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17641676/" } ], "x": 5948.666666666667, "y": 2859.3026315789475, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423632", "label": "AFXBO binds DNA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423632", "summary": "AFXBO binds DNA is a Reactome release 97 curated reaction event. The source record connects AFXBO, DNA to AFXBO:DNA in nucleoplasm.", "equation": "AFXBO + DNA → AFXBO:DNA", "inputs": [ { "node_id": "R-ALL-5423619", "reactome_id": "R-ALL-5423619", "label": "AFXBO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30725", "formula": "C17H12O7", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29428", "reactome_id": "R-ALL-29428", "label": "DNA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5423670", "reactome_id": "R-ALL-5423670", "label": "AFXBO:DNA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8448352", "title": "DNA conformation mediates aflatoxin B1-DNA binding and the formation of guanine N7 adducts by aflatoxin B1 8,9-exo-epoxide", "journal": "Chem. Res. Toxicol.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8448352/" }, { "id": "PMID:16458422", "title": "Aflatoxin B1-induced DNA damage and its repair", "journal": "Cancer Lett.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16458422/" } ], "x": 7461.333333333333, "y": 3172.9313725490197, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423637", "label": "AKR dimers reduce AFBDHO to AFBDOH", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423637", "summary": "AKR dimers reduce AFBDHO to AFBDOH is a Reactome release 97 curated reaction event. The source record connects NADPH, H+, AFBDHO to NADP+, AFBDOH in cytosol.", "equation": "NADPH + H+ + AFBDHO → NADP+ + AFBDOH", "inputs": [ { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5423727", "reactome_id": "R-ALL-5423727", "label": "AFBDHO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:53107", "formula": "C17H14O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5423729", "reactome_id": "R-ALL-5423729", "label": "AFBDOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:53108", "formula": "C17H18O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of AKR dimers [cytosol]", "activity_id": "5423725", "ec_numbers": [ "1.1.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9576847", "title": "Molecular cloning, expression and catalytic activity of a human AKR7 member of the aldo-keto reductase superfamily: evidence that the major 2-carboxybenzaldehyde reductase from human liver is a homologue of rat aflatoxin B1-aldehyde reductase", "journal": "Biochem. J.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9576847/" }, { "id": "PMID:18416522", "title": "Protection against aflatoxin B1-induced cytotoxicity by expression of the cloned aflatoxin B1-aldehyde reductases rat AKR7A1 and human AKR7A3", "journal": "Chem. Res. Toxicol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18416522/" }, { "id": "PMID:12727802", "title": "Characterization of the rat aflatoxin B1 aldehyde reductase gene, AKR7A1. Structure and chromosomal localization of AKR7A1 as well as identification of antioxidant response elements in the gene promoter", "journal": "Carcinogenesis", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12727802/" }, { "id": "PMID:16970545", "title": "Aldo-keto reductases and bioactivation/detoxication", "journal": "Annu. Rev. Pharmacol. Toxicol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/16970545/" }, { "id": "PMID:11409944", "title": "Reduction of aflatoxin B1 dialdehyde by rat and human aldo-keto reductases", "journal": "Chem. Res. Toxicol.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11409944/" } ], "x": 7740, "y": 3066.1470588235293, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423647", "label": "CYP2A13 oxidises AFM1 to AFM1E", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423647", "summary": "CYP2A13 oxidises AFM1 to AFM1E is a Reactome release 97 curated reaction event. The source record connects AFM1, H+, NADPH to AFM1E, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "AFM1 + H+ + NADPH + O2 → AFM1E + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-5423629", "reactome_id": "R-ALL-5423629", "label": "AFM1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78576", "formula": "C17H12O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5423657", "reactome_id": "R-ALL-5423657", "label": "AFM1E", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78577", "formula": "C17H12O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP2A13 [endoplasmic reticulum membrane]", "activity_id": "5423682", "ec_numbers": null, "accessions": [ "Q16696" ] } ], "citations": [ { "id": "PMID:16385575", "title": "Efficient activation of aflatoxin B1 by cytochrome P450 2A13, an enzyme predominantly expressed in human respiratory tract", "journal": "Int. J. Cancer", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16385575/" } ], "x": 7740, "y": 3012.7549019607845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423653", "label": "MGST trimers transfer GS from GSH to AFXBO and AFNBO", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423653", "summary": "MGST trimers transfer GS from GSH to AFXBO and AFNBO is a Reactome release 97 curated reaction event. The source record connects AFXBO,AFNBO, GSH to AFXBO-SG,AFNBO-SG in cytosol · endoplasmic reticulum membrane.", "equation": "AFXBO,AFNBO + GSH → AFXBO-SG,AFNBO-SG", "inputs": [ { "node_id": "R-ALL-5423723", "reactome_id": "R-ALL-5423723", "label": "AFXBO,AFNBO", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5423730", "reactome_id": "R-ALL-5423730", "label": "AFXBO-SG,AFNBO-SG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "glutathione transferase activity of MGST trimers [endoplasmic reticulum membrane]", "activity_id": "158544", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:3372534", "title": "Gene expression of rat and human microsomal glutathione S-transferases", "journal": "J. Biol. Chem.", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3372534/" }, { "id": "PMID:8812420", "title": "Structural organization of the human microsomal glutathione S-transferase gene (GST12)", "journal": "Genomics", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8812420/" }, { "id": "PMID:8703034", "title": "Identification and characterization of a novel human microsomal glutathione S-transferase with leukotriene C4 synthase activity and significant sequence identity to 5-lipoxygenase-activating protein and leukotriene C4 synthase", "journal": "J. Biol. Chem.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8703034/" }, { "id": "PMID:9278457", "title": "Identification and characterization of a novel microsomal enzyme with glutathione-dependent transferase and peroxidase activities", "journal": "J. Biol. Chem.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9278457/" }, { "id": "PMID:11904223", "title": "The 3-D structure of microsomal glutathione transferase 1 at 6 A resolution as determined by electron crystallography of p22(1)2(1) crystals", "journal": "Biochim. Biophys. Acta", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11904223/" } ], "x": 7740, "y": 3142.421568627451, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423656", "label": "AFXBO hydrolyses non-enzymatically to AFBDHD", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423656", "summary": "AFXBO hydrolyses non-enzymatically to AFBDHD is a Reactome release 97 curated reaction event. The source record connects H2O, AFXBO to AFBDHD in cytosol.", "equation": "H2O + AFXBO → AFBDHD", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159211", "reactome_id": "R-ALL-159211", "label": "AFXBO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30725", "formula": "C17H12O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5423726", "reactome_id": "R-ALL-5423726", "label": "AFBDHD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:53106", "formula": "C17H14O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9208174", "title": "Aflatoxin B1 8,9-epoxide hydrolysis in the presence of rat and human epoxide hydrolase", "journal": "Chem. Res. Toxicol.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9208174/" }, { "id": "PMID:9675258", "title": "Activation and detoxication of aflatoxin B1", "journal": "Mutat. Res.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9675258/" } ], "x": 7740, "y": 3150.049019607843, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423664", "label": "CYP3A4,5 hydroxylates AFB1 to AFQ1", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423664", "summary": "CYP3A4,5 hydroxylates AFB1 to AFQ1 is a Reactome release 97 curated reaction event. The source record connects AFB1, NADPH, O2 to AFQ1, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "AFB1 + NADPH + O2 + H+ → AFQ1 + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-5423605", "reactome_id": "R-ALL-5423605", "label": "AFB1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2504", "formula": "C17H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5423660", "reactome_id": "R-ALL-5423660", "label": "AFQ1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78582", "formula": "C17H12O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP3A4,5 [endoplasmic reticulum membrane]", "activity_id": "5423671", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8975785", "title": "The kinetics of aflatoxin B1 oxidation by human cDNA-expressed and human liver microsomal cytochromes P450 1A2 and 3A4", "journal": "Toxicol. Appl. Pharmacol.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8975785/" }, { "id": "PMID:3931076", "title": "Aflatoxin metabolism in humans: detection of metabolites and nucleic acid adducts in urine by affinity chromatography", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3931076/" }, { "id": "PMID:1643250", "title": "Oxidation of aflatoxins and sterigmatocystin by human liver microsomes: significance of aflatoxin Q1 as a detoxication product of aflatoxin B1", "journal": "Chem. Res. Toxicol.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1643250/" } ], "x": 8018.666666666667, "y": 3012.7549019607845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423672", "label": "CYP1A2, 3A4 oxidise AFB1 to AFNBO", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423672", "summary": "CYP1A2, 3A4 oxidise AFB1 to AFNBO is a Reactome release 97 curated reaction event. The source record connects AFB1, H+, NADPH to AFNBO, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "AFB1 + H+ + NADPH + O2 → AFNBO + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-5423605", "reactome_id": "R-ALL-5423605", "label": "AFB1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2504", "formula": "C17H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5423663", "reactome_id": "R-ALL-5423663", "label": "AFNBO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78586", "formula": "C17H12O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A2,3A4 [endoplasmic reticulum membrane]", "activity_id": "5423617", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:1504254", "title": "The endo-8,9-epoxide of aflatoxin B1: a new metabolite", "journal": "Chem. Res. Toxicol.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1504254/" }, { "id": "PMID:7766804", "title": "Oxidation of aflatoxin B1 by bacterial recombinant human cytochrome P450 enzymes", "journal": "Chem. Res. Toxicol.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7766804/" } ], "x": 7461.333333333333, "y": 3020.3823529411766, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423678", "label": "CYP1A2 hydroxylates AFB1 to AFM1", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423678", "summary": "CYP1A2 hydroxylates AFB1 to AFM1 is a Reactome release 97 curated reaction event. The source record connects AFB1, H+, NADPH to AFM1, H2O, NADP+ in endoplasmic reticulum membrane · cytosol.", "equation": "AFB1 + H+ + NADPH + O2 → AFM1 + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-5423605", "reactome_id": "R-ALL-5423605", "label": "AFB1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2504", "formula": "C17H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5423629", "reactome_id": "R-ALL-5423629", "label": "AFM1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78576", "formula": "C17H12O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A2 [endoplasmic reticulum membrane]", "activity_id": "5423677", "ec_numbers": null, "accessions": [ "P05177" ] } ], "citations": [ { "id": "PMID:7766804", "title": "Oxidation of aflatoxin B1 by bacterial recombinant human cytochrome P450 enzymes", "journal": "Chem. Res. Toxicol.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7766804/" } ], "x": 7740, "y": 3020.3823529411766, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423694", "label": "AFBDHD hydrolyses non-enzymatically to AFBDHO", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423694", "summary": "AFBDHD hydrolyses non-enzymatically to AFBDHO is a Reactome release 97 curated reaction event. The source record connects AFBDHD, H2O to AFBDHO in cytosol.", "equation": "AFBDHD + H2O → AFBDHO", "inputs": [ { "node_id": "R-ALL-5423726", "reactome_id": "R-ALL-5423726", "label": "AFBDHD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:53106", "formula": "C17H14O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5423727", "reactome_id": "R-ALL-5423727", "label": "AFBDHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:53107", "formula": "C17H14O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9675258", "title": "Activation and detoxication of aflatoxin B1", "journal": "Mutat. Res.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9675258/" } ], "x": 8018.666666666667, "y": 3150.049019607843, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5423728", "label": "AFXBO translocates from cytosol to nucleoplasm", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5423728", "summary": "AFXBO translocates from cytosol to nucleoplasm is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects AFXBO to AFXBO in cytosol · nucleoplasm.", "equation": "AFXBO → AFXBO", "inputs": [ { "node_id": "R-ALL-159211", "reactome_id": "R-ALL-159211", "label": "AFXBO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30725", "formula": "C17H12O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5423619", "reactome_id": "R-ALL-5423619", "label": "AFXBO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30725", "formula": "C17H12O7", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9675258", "title": "Activation and detoxication of aflatoxin B1", "journal": "Mutat. Res.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9675258/" } ], "x": 7461.333333333333, "y": 3188.1862745098038, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-5433066", "label": "Unknown NAT transfers COCH3 to AFXBO-C, AFNBO-C", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19214" ], "source_url": "https://reactome.org/content/detail/R-HSA-5433066", "summary": "Unknown NAT transfers COCH3 to AFXBO-C, AFNBO-C is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects AFXBO-C,AFNBO-C, Ac-CoA to CoA-SH, AFXBO-NAC,AFNBO-NAC, H+ in cytosol and cross-references RHEA:19214.", "equation": "AFXBO-C,AFNBO-C + Ac-CoA → CoA-SH + AFXBO-NAC,AFNBO-NAC + H+", "inputs": [ { "node_id": "R-ALL-5490231", "reactome_id": "R-ALL-5490231", "label": "AFXBO-C,AFNBO-C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5490236", "reactome_id": "R-ALL-5490236", "label": "AFXBO-NAC,AFNBO-NAC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-cysteine-S-conjugate N-acetyltransferase activity of unknown NAT [cytosol]", "activity_id": "5490256", "ec_numbers": [ "2.3.1.80" ], "accessions": [] } ], "citations": [ { "id": "PMID:8145281", "title": "Glutathione conjugation and conversion to mercapturic acids can occur as an intrahepatic process", "journal": "J Toxicol Environ Health", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8145281/" } ], "x": 8018.666666666667, "y": 2936.4803921568628, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-5433067", "label": "DPEPs hydrolyse glycine from AFXBO-CG, AFNBO-CG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5433067", "summary": "DPEPs hydrolyse glycine from AFXBO-CG, AFNBO-CG is a Reactome release 97 curated reaction event. The source record connects AFXBO-CG,AFNBO-CG to AFXBO-C,AFNBO-C, Gly in plasma membrane · extracellular region.", "equation": "AFXBO-CG,AFNBO-CG → AFXBO-C,AFNBO-C + Gly", "inputs": [ { "node_id": "R-ALL-5490235", "reactome_id": "R-ALL-5490235", "label": "AFXBO-CG,AFNBO-CG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5490250", "reactome_id": "R-ALL-5490250", "label": "AFXBO-C,AFNBO-C", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-266029", "reactome_id": "R-ALL-266029", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dipeptidase activity of DPEP1,2 dimers [plasma membrane]", "activity_id": "266039", "ec_numbers": [ "3.4.13.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:32325220", "title": "Structure of human DPEP3 in complex with the SC-003 antibody Fab fragment reveals basis for lack of dipeptidase activity", "journal": "J Struct Biol", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32325220/" }, { "id": "PMID:12144777", "title": "Crystal structure of human renal dipeptidase involved in beta-lactam hydrolysis", "journal": "J. Mol. Biol.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12144777/" }, { "id": "PMID:8439558", "title": "Cloning and structural analysis of genomic DNA for human renal dipeptidase", "journal": "Biochim. Biophys. Acta", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8439558/" }, { "id": "PMID:7764673", "title": "Gene structural analysis and expression of human renal dipeptidase", "journal": "Biotechnol. Prog.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7764673/" } ], "x": 8018.666666666667, "y": 3142.421568627451, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5433072", "label": "GGTs hydrolyse glutamate from AFXBO-SG, AFNBO-SG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:59469" ], "source_url": "https://reactome.org/content/detail/R-HSA-5433072", "summary": "GGTs hydrolyse glutamate from AFXBO-SG, AFNBO-SG is a Reactome release 97 curated reaction event. The source record connects AFXBO-SG,AFNBO-SG, H2O to AFXBO-CG,AFNBO-CG, L-Glu in extracellular region · plasma membrane and cross-references RHEA:59469.", "equation": "AFXBO-SG,AFNBO-SG + H2O → AFXBO-CG,AFNBO-CG + L-Glu", "inputs": [ { "node_id": "R-ALL-5490253", "reactome_id": "R-ALL-5490253", "label": "AFXBO-SG,AFNBO-SG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5490235", "reactome_id": "R-ALL-5490235", "label": "AFXBO-CG,AFNBO-CG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-210382", "reactome_id": "R-ALL-210382", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione gamma-glutamate hydrolase activity of GGT dimers [plasma membrane]", "activity_id": "8943271", "ec_numbers": [ "3.4.19.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:2573352", "title": "Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney", "journal": "Biochem Biophys Res Commun", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2573352/" }, { "id": "PMID:19463", "title": "Human kidney gamma-glutamyl transpeptidase. Catalytic properties, subunit structure, and localization of the gamma-glutamyl binding site on the light subunit", "journal": "J Biol Chem", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/19463/" }, { "id": "PMID:18357469", "title": "The human gamma-glutamyltransferase gene family", "journal": "Hum Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18357469/" } ], "x": 8018.666666666667, "y": 2928.8529411764707, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5433074", "label": "ACY1:Zn2+ dimer hydrolyses mercapturic acids", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:36856" ], "source_url": "https://reactome.org/content/detail/R-HSA-5433074", "summary": "ACY1:Zn2+ dimer hydrolyses mercapturic acids is a Reactome release 97 curated reaction event. The source record connects AFXBO-NAC,AFNBO-NAC, H2O to AFXBO-C,AFNBO-C, acetate in cytosol and cross-references RHEA:36856.", "equation": "AFXBO-NAC,AFNBO-NAC + H2O → AFXBO-C,AFNBO-C + acetate", "inputs": [ { "node_id": "R-ALL-5490236", "reactome_id": "R-ALL-5490236", "label": "AFXBO-NAC,AFNBO-NAC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5490231", "reactome_id": "R-ALL-5490231", "label": "AFXBO-C,AFNBO-C", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-9717939", "reactome_id": "R-ALL-9717939", "label": "acetate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aminoacylase activity of ACY1:Zn2+ dimer [cytosol]", "activity_id": "5433071", "ec_numbers": [ "3.5.1.14" ], "accessions": [] } ], "citations": [ { "id": "PMID:12933810", "title": "Essential roles of zinc ligation and enzyme dimerization for catalysis in the aminoacylase-1/M20 family", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12933810/" } ], "x": 7461.333333333333, "y": 2936.4803921568628, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-548800", "label": "ELOVL1,2,3,5 elongate AA-CoA and Mal-CoA to 3ODCT-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:36476" ], "source_url": "https://reactome.org/content/detail/R-HSA-548800", "summary": "ELOVL1,2,3,5 elongate AA-CoA and Mal-CoA to 3ODCT-CoA is a Reactome release 97 curated reaction event. The source record connects AA-CoA, Mal-CoA, H+ to 3ODCT-CoA, CoA-SH, CO2 in cytosol · endoplasmic reticulum membrane and cross-references RHEA:36476.", "equation": "AA-CoA + Mal-CoA + H+ → 3ODCT-CoA + CoA-SH + CO2", "inputs": [ { "node_id": "R-ALL-548819", "reactome_id": "R-ALL-548819", "label": "AA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57368", "formula": "C41H62N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29508", "reactome_id": "R-ALL-29508", "label": "Mal-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-548816", "reactome_id": "R-ALL-548816", "label": "3ODCT-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73852", "formula": "C43H64N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL1,2,3,5 [endoplasmic reticulum membrane]", "activity_id": "548806", "ec_numbers": [ "2.3.1.199" ], "accessions": [] } ], "citations": [ { "id": "PMID:12371743", "title": "Identification and expression of mammalian long-chain PUFA elongation enzymes", "journal": "Lipids", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12371743/" }, { "id": "PMID:20937905", "title": "ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis", "journal": "Proc Natl Acad Sci U S A", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20937905/" } ], "x": 590, "y": 1664.2631578947369, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-548814", "label": "ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:35316" ], "source_url": "https://reactome.org/content/detail/R-HSA-548814", "summary": "ELOVL3,6,7 elongate PALM-CoA and Mal-CoA to 3OOD-CoA is a Reactome release 97 curated reaction event. The source record connects PALM-CoA, Mal-CoA, H+ to 3OOD-CoA, CoA-SH, CO2 in cytosol · endoplasmic reticulum membrane and cross-references RHEA:35316.", "equation": "PALM-CoA + Mal-CoA + H+ → 3OOD-CoA + CoA-SH + CO2", "inputs": [ { "node_id": "R-ALL-200993", "reactome_id": "R-ALL-200993", "label": "PALM-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57379", "formula": "C37H62N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29508", "reactome_id": "R-ALL-29508", "label": "Mal-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-548812", "reactome_id": "R-ALL-548812", "label": "3OOD-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:71407", "formula": "C39H64N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL3,6,7 [endoplasmic reticulum membrane]", "activity_id": "548801", "ec_numbers": [ "2.3.1.199" ], "accessions": [] } ], "citations": [ { "id": "PMID:19505953", "title": "Identification and characterization of a selective radioligand for ELOVL6", "journal": "J Biochem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19505953/" }, { "id": "PMID:21959040", "title": "Biochemical characterization of the very long-chain fatty acid elongase ELOVL7", "journal": "FEBS Lett.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21959040/" }, { "id": "PMID:20937905", "title": "ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis", "journal": "Proc Natl Acad Sci U S A", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20937905/" } ], "x": 868.6666666666667, "y": 1664.2631578947369, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-548815", "label": "ELOVL7 elongates ICS-CoA and Mal-CoA to 3ODC-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:35328" ], "source_url": "https://reactome.org/content/detail/R-HSA-548815", "summary": "ELOVL7 elongates ICS-CoA and Mal-CoA to 3ODC-CoA is a Reactome release 97 curated reaction event. The source record connects ICS-CoA, Mal-CoA, H+ to CoA-SH, CO2, 3ODC-CoA in cytosol · endoplasmic reticulum membrane and cross-references RHEA:35328.", "equation": "ICS-CoA + Mal-CoA + H+ → CoA-SH + CO2 + 3ODC-CoA", "inputs": [ { "node_id": "R-ALL-548794", "reactome_id": "R-ALL-548794", "label": "ICS-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57380", "formula": "C41H70N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29508", "reactome_id": "R-ALL-29508", "label": "Mal-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-548829", "reactome_id": "R-ALL-548829", "label": "3ODC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:71451", "formula": "C43H72N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL7 [endoplasmic reticulum membrane]", "activity_id": "548807", "ec_numbers": [ "2.3.1.199" ], "accessions": [ "A1L3X0" ] } ], "citations": [ { "id": "PMID:19826053", "title": "Novel lipogenic enzyme ELOVL7 is involved in prostate cancer growth through saturated long-chain fatty acid metabolism", "journal": "Cancer Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19826053/" } ], "x": 311.33333333333337, "y": 1667.280701754386, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-548818", "label": "HSD17B3,12 hydrogenates 3OOD-CoA to 3HODC-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:35301" ], "source_url": "https://reactome.org/content/detail/R-HSA-548818", "summary": "HSD17B3,12 hydrogenates 3OOD-CoA to 3HODC-CoA is a Reactome release 97 curated reaction event. The source record connects 3OOD-CoA, NADPH, H+ to 3HODC-CoA, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:35301.", "equation": "3OOD-CoA + NADPH + H+ → 3HODC-CoA + NADP+", "inputs": [ { "node_id": "R-ALL-548812", "reactome_id": "R-ALL-548812", "label": "3OOD-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:71407", "formula": "C39H64N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-548836", "reactome_id": "R-ALL-548836", "label": "3HODC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:71408", "formula": "C39H66N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "very-long-chain 3-oxoacyl-CoA reductase activity of HSD17B3,12 [endoplasmic reticulum membrane]", "activity_id": "548828", "ec_numbers": [ "1.1.1.330" ], "accessions": [] } ], "citations": [ { "id": "PMID:8075637", "title": "Male pseudohermaphroditism caused by mutations of testicular 17 beta-hydroxysteroid dehydrogenase 3", "journal": "Nat Genet", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8075637/" }, { "id": "PMID:12482854", "title": "Identification of two mammalian reductases involved in the two-carbon fatty acyl elongation cascade", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12482854/" } ], "x": 590, "y": 1670.298245614035, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-548830", "label": "ELOVL1,4 elongate TCS-CoA and Mal-CoA to 3OHC-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:36516" ], "source_url": "https://reactome.org/content/detail/R-HSA-548830", "summary": "ELOVL1,4 elongate TCS-CoA and Mal-CoA to 3OHC-CoA is a Reactome release 97 curated reaction event. The source record connects TCS-CoA, Mal-CoA, H+ to 3OHC-CoA, CoA-SH, CO2 in cytosol · endoplasmic reticulum membrane and cross-references RHEA:36516.", "equation": "TCS-CoA + Mal-CoA + H+ → 3OHC-CoA + CoA-SH + CO2", "inputs": [ { "node_id": "R-ALL-548808", "reactome_id": "R-ALL-548808", "label": "TCS-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65052", "formula": "C45H78N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29508", "reactome_id": "R-ALL-29508", "label": "Mal-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-548797", "reactome_id": "R-ALL-548797", "label": "3OHC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73980", "formula": "C47H80N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fatty acid elongase activity of ELOVL1,4 [endoplasmic reticulum membrane]", "activity_id": "548824", "ec_numbers": [ "2.3.1.199" ], "accessions": [] } ], "citations": [ { "id": "PMID:18728184", "title": "Role of Stargardt-3 macular dystrophy protein (ELOVL4) in the biosynthesis of very long chain fatty acids", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18728184/" }, { "id": "PMID:16036915", "title": "Dominant negative mechanism underlies autosomal dominant Stargardt-like macular dystrophy linked to mutations in ELOVL4", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16036915/" } ], "x": 590, "y": 1667.280701754386, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-548831", "label": "TECR,TECRL dehydrogenate TOD-CoA to ST-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:35353" ], "source_url": "https://reactome.org/content/detail/R-HSA-548831", "summary": "TECR,TECRL dehydrogenate TOD-CoA to ST-CoA is a Reactome release 97 curated reaction event. The source record connects TOD-CoA, NADPH, H+ to ST-CoA, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:35353.", "equation": "TOD-CoA + NADPH + H+ → ST-CoA + NADP+", "inputs": [ { "node_id": "R-ALL-548809", "reactome_id": "R-ALL-548809", "label": "TOD-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:71412", "formula": "C39H64N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-428139", "reactome_id": "R-ALL-428139", "label": "ST-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57394", "formula": "C39H66N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "very-long-chain fatty acyl-CoA dehydrogenase activity of TECR,TECRL [endoplasmic reticulum membrane]", "activity_id": "548793", "ec_numbers": [ "1.3.8.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:12482854", "title": "Identification of two mammalian reductases involved in the two-carbon fatty acyl elongation cascade", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12482854/" } ], "x": 868.6666666666667, "y": 1670.298245614035, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-548843", "label": "ACSL3,4 ligate CoA to AA to form AA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:19714" ], "source_url": "https://reactome.org/content/detail/R-HSA-548843", "summary": "ACSL3,4 ligate CoA to AA to form AA-CoA is a Reactome release 97 curated reaction event. The source record connects AA, ATP, CoA-SH to AA-CoA, AMP, PPi in cytosol · endoplasmic reticulum membrane and cross-references RHEA:19714.", "equation": "AA + ATP + CoA-SH → AA-CoA + AMP + PPi + H2O", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-548819", "reactome_id": "R-ALL-548819", "label": "AA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57368", "formula": "C41H62N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of ACSL3,4 [endoplasmic reticulum membrane]", "activity_id": "548838", "ec_numbers": [ "6.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:12525535", "title": "A third MRX family (MRX68) is the result of mutation in the long chain fatty acid-CoA ligase 4 (FACL4) gene: proposal of a rapid enzymatic assay for screening mentally retarded patients", "journal": "J Med Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12525535/" }, { "id": "PMID:11889465", "title": "FACL4, encoding fatty acid-CoA ligase 4, is mutated in nonspecific X-linked mental retardation", "journal": "Nat Genet", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11889465/" }, { "id": "PMID:18003621", "title": "Long chain acyl-CoA synthetase 3-mediated phosphatidylcholine synthesis is required for assembly of very low density lipoproteins in human hepatoma Huh7 cells", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18003621/" } ], "x": 311.33333333333337, "y": 1655.2105263157894, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5490230", "label": "AFXBO-SG, AFNBO-SG translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5490230", "summary": "AFXBO-SG, AFNBO-SG translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects AFXBO-SG,AFNBO-SG to AFXBO-SG,AFNBO-SG in cytosol · extracellular region.", "equation": "AFXBO-SG,AFNBO-SG → AFXBO-SG,AFNBO-SG", "inputs": [ { "node_id": "R-ALL-5423730", "reactome_id": "R-ALL-5423730", "label": "AFXBO-SG,AFNBO-SG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5490253", "reactome_id": "R-ALL-5490253", "label": "AFXBO-SG,AFNBO-SG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:18357469", "title": "The human gamma-glutamyltransferase gene family", "journal": "Hum Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18357469/" } ], "x": 7740, "y": 3188.1862745098038, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-5490269", "label": "AFXBO-C, AFNBO-C translocate from extracellular region to cytosol", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "extracellular region · cytosol", "compartment_ids": [ "GO:0005576", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5490269", "summary": "AFXBO-C, AFNBO-C translocate from extracellular region to cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects AFXBO-C,AFNBO-C to AFXBO-C,AFNBO-C in extracellular region · cytosol.", "equation": "AFXBO-C,AFNBO-C → AFXBO-C,AFNBO-C", "inputs": [ { "node_id": "R-ALL-5490250", "reactome_id": "R-ALL-5490250", "label": "AFXBO-C,AFNBO-C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5490231", "reactome_id": "R-ALL-5490231", "label": "AFXBO-C,AFNBO-C", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9675258", "title": "Activation and detoxication of aflatoxin B1", "journal": "Mutat. Res.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9675258/" }, { "id": "PMID:19514968", "title": "Biological degradation of aflatoxins", "journal": "Drug Metab. Rev.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19514968/" } ], "x": 8018.666666666667, "y": 3188.1862745098038, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-549112", "label": "G3P is acylated to 1-acyl LPA by AGPAT6", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:15326" ], "source_url": "https://reactome.org/content/detail/R-HSA-549112", "summary": "G3P is acylated to 1-acyl LPA by AGPAT6 is a Reactome release 97 curated reaction event. The source record connects G3P, acyl-CoA to 1-acyl LPA, CoA-SH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:15326.", "equation": "G3P + acyl-CoA → 1-acyl LPA + CoA-SH", "inputs": [ { "node_id": "R-ALL-76114", "reactome_id": "R-ALL-76114", "label": "G3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500604", "reactome_id": "R-ALL-1500604", "label": "1-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycerol-3-phosphate O-acyltransferase activity of AGPAT6 [endoplasmic reticulum membrane]", "activity_id": "1500613", "ec_numbers": [ "2.3.1.15" ], "accessions": [ "Q86UL3" ] } ], "citations": [ { "id": "PMID:17170135", "title": "Molecular identification of microsomal acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17170135/" }, { "id": "PMID:18238778", "title": "AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18238778/" }, { "id": "PMID:18718904", "title": "Acyl-CoA:lysophospholipid acyltransferases", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18718904/" } ], "x": 2938.666666666667, "y": 1636.9109589041095, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-549192", "label": "1,2-diacyl-glycerol + acyl-CoA =\u003e triacylglycerol + CoASH [DGAT2]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-549192", "summary": "1,2-diacyl-glycerol + acyl-CoA =\u003e triacylglycerol + CoASH [DGAT2] is a Reactome release 97 curated reaction event. The source record connects DAG, FACoA to TAG, CoA-SH in cytosol · endoplasmic reticulum membrane.", "equation": "DAG + FACoA → TAG + CoA-SH", "inputs": [ { "node_id": "R-ALL-76103", "reactome_id": "R-ALL-76103", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-75967", "reactome_id": "R-ALL-75967", "label": "FACoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77636", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-549167", "reactome_id": "R-ALL-549167", "label": "TAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diacylglycerol O-acyltransferase activity of DGAT2 [endoplasmic reticulum membrane]", "activity_id": "549191", "ec_numbers": [ "2.3.1.20" ], "accessions": [ "Q96PD7" ] } ], "citations": [ { "id": "PMID:11481335", "title": "Cloning of DGAT2, a second mammalian diacylglycerol acyltransferase, and related family members", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11481335/" }, { "id": "PMID:14521909", "title": "A novel diacylglycerol acyltransferase (DGAT2) is decreased in human psoriatic skin and increased in diabetic mice", "journal": "Biochem Biophys Res Commun", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14521909/" } ], "x": 3878.666666666667, "y": 2182.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5602295", "label": "CYP4F22 20-hydroxylates TrXA3", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5602295", "summary": "CYP4F22 20-hydroxylates TrXA3 is a Reactome release 97 curated reaction event. The source record connects H+, NADPH, O2 to H2O, NADP+, 20OH-TrXA3 in endoplasmic reticulum membrane · cytosol.", "equation": "H+ + NADPH + O2 + TrXA3 → H2O + NADP+ + 20OH-TrXA3", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2142713", "reactome_id": "R-ALL-2142713", "label": "TrXA3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57448", "formula": "C20H33O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5602281", "reactome_id": "R-ALL-5602281", "label": "20OH-TrXA3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83782", "formula": "C20H34O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP4F22 [endoplasmic reticulum membrane]", "activity_id": "5602288", "ec_numbers": null, "accessions": [ "Q6NT55" ] } ], "citations": [ { "id": "PMID:16436457", "title": "Mutations in a new cytochrome P450 gene in lamellar ichthyosis type 3", "journal": "Hum Mol Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16436457/" } ], "x": 8018.666666666667, "y": 3020.3823529411766, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5603114", "label": "MAT2B:MAT2A:K+:2Mg2+ transfers Ado from ATP to L-Met", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21081" ], "source_url": "https://reactome.org/content/detail/R-HSA-5603114", "summary": "MAT2B:MAT2A:K+:2Mg2+ transfers Ado from ATP to L-Met is a Reactome release 97 curated reaction event. The source record connects ATP, L-Met, H2O to AdoMet, Pi, PPi in cytosol and cross-references RHEA:21081.", "equation": "ATP + L-Met + H2O → AdoMet + Pi + PPi", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-174390", "reactome_id": "R-ALL-174390", "label": "L-Met", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57844", "formula": "C5H11NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methionine adenosyltransferase activity of MAT2B:MAT2A:K+:2Mg2+ [cytosol]", "activity_id": "5603117", "ec_numbers": [ "2.5.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:10644686", "title": "Cloning, expression, and functional characterization of the beta regulatory subunit of human methionine adenosyltransferase (MAT II)", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10644686/" }, { "id": "PMID:10514445", "title": "Expression and functional interaction of the catalytic and regulatory subunits of human methionine adenosyltransferase in mammalian cells", "journal": "J. Biol. Chem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10514445/" } ], "x": 7740, "y": 2844.950980392157, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5652172", "label": "AKR1B1 reduces Glc to D-sorbitol", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "fructose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:59926" ], "source_url": "https://reactome.org/content/detail/R-HSA-5652172", "summary": "AKR1B1 reduces Glc to D-sorbitol is a Reactome release 97 curated reaction event. The source record connects Glc, NADPH, H+ to D-sorbitol, NADP+ in cytosol and cross-references RHEA:59926.", "equation": "Glc + NADPH + H+ → D-sorbitol + NADP+", "inputs": [ { "node_id": "R-ALL-70113", "reactome_id": "R-ALL-70113", "label": "Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5652213", "reactome_id": "R-ALL-5652213", "label": "D-sorbitol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17924", "formula": "C6H14O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldose reductase (NADPH) activity of AKR1B1 [cytosol]", "activity_id": "196106", "ec_numbers": [ "1.1.1.21" ], "accessions": [ "P15121" ] } ], "citations": [ { "id": "PMID:1420136", "title": "Aldose reductase: model for a new paradigm of enzymic perfection in detoxification catalysts", "journal": "Biochemistry", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1420136/" }, { "id": "PMID:14401390", "title": "[Aldose reductase]", "journal": "Biochim. Biophys. Acta", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/14401390/" }, { "id": "PMID:8281941", "title": "Sequence of pig lens aldose reductase and electrospray mass spectrometry of non-covalent and covalent complexes", "journal": "Eur. J. Biochem.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8281941/" }, { "id": "PMID:2112546", "title": "Cloning and expression of human aldose reductase", "journal": "J. Biol. Chem.", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2112546/" }, { "id": "PMID:18220710", "title": "Aldose reductase, still a compelling target for diabetic neuropathy", "journal": "Curr Drug Targets", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18220710/" }, { "id": "PMID:15272156", "title": "The crystallographic structure of the aldose reductase-IDD552 complex shows direct proton donation from tyrosine 48", "journal": "Acta Crystallogr. D Biol. Crystallogr.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15272156/" } ], "x": 2660, "y": 327.8333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5652195", "label": "SORD oxidizes D-sorbitol to Fru", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "fructose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:33033" ], "source_url": "https://reactome.org/content/detail/R-HSA-5652195", "summary": "SORD oxidizes D-sorbitol to Fru is a Reactome release 97 curated reaction event. The source record connects D-sorbitol, NAD+ to NADH, H+, keto-D-Fru in cytosol and cross-references RHEA:33033.", "equation": "D-sorbitol + NAD+ → NADH + H+ + keto-D-Fru", "inputs": [ { "node_id": "R-ALL-5652213", "reactome_id": "R-ALL-5652213", "label": "D-sorbitol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17924", "formula": "C6H14O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9832975", "reactome_id": "R-ALL-9832975", "label": "keto-D-Fru", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:48095", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-iditol 2-dehydrogenase (NAD+) activity of SORD tetramer [cytosol]", "activity_id": "5652209", "ec_numbers": [ "1.1.1.14" ], "accessions": [] } ], "citations": [ { "id": "PMID:14401391", "title": "[The mechanism of the formation of seminal fructose and fetal fructose]", "journal": "Biochim. Biophys. Acta", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/14401391/" }, { "id": "PMID:2691249", "title": "Variability within mammalian sorbitol dehydrogenases. The primary structure of the human liver enzyme", "journal": "Eur. J. Biochem.", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2691249/" }, { "id": "PMID:6870831", "title": "Polyol-pathway enzymes of human brain. Partial purification and properties of sorbitol dehydrogenase", "journal": "Biochem. J.", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6870831/" }, { "id": "PMID:12962626", "title": "X-ray crystallographic and kinetic studies of human sorbitol dehydrogenase", "journal": "Structure", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12962626/" } ], "x": 2938.666666666667, "y": 327.8333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5655955", "label": "OLAH hydrolyzes decanoyl-FASN dimer to DECA and FASN dimer", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:30116" ], "source_url": "https://reactome.org/content/detail/R-HSA-5655955", "summary": "OLAH hydrolyzes decanoyl-FASN dimer to DECA and FASN dimer is a Reactome release 97 curated reaction event. The source record connects H2O, decanoyl-FASN dimer to DECA, FASN dimer, H+ in cytosol and cross-references RHEA:30116.", "equation": "2 H2O + decanoyl-FASN dimer → 2 DECA + FASN dimer + 2 H+", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-5655943", "reactome_id": "R-HSA-5655943", "label": "decanoyl-FASN dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-879620", "reactome_id": "R-ALL-879620", "label": "DECA", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:27689", "formula": "C10H19O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-77380", "reactome_id": "R-HSA-77380", "label": "FASN dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acyl-[ACP] hydrolase activity of OLAH [cytosol]", "activity_id": "5655933", "ec_numbers": [ "3.1.2.14" ], "accessions": [ "Q9NV23" ] } ], "citations": [ { "id": "PMID:627544", "title": "Purification and properties of a thioesterase from lactating rat mammary gland which modifies the product specificity of fatty acid synthetase", "journal": "J. Biol. Chem.", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/627544/" }, { "id": "PMID:3805044", "title": "Interaction of rat mammary gland thioesterase II with fatty acid synthetase is dependent on the presence of acyl chains on the synthetase", "journal": "J. Biol. Chem.", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3805044/" }, { "id": "PMID:13651131", "title": "The fatty acids of human milk from mothers on diets taken ad libitum", "journal": "Biochem. J.", "year": 1959, "url": "https://pubmed.ncbi.nlm.nih.gov/13651131/" }, { "id": "PMID:5810867", "title": "Triglyceride structure of human milk fat", "journal": "Can. J. Biochem.", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5810867/" } ], "x": 868.6666666666667, "y": 1685.3859649122808, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5676637", "label": "PTPLs dehydrate VLC3HA-CoA to VLCTDA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:26527" ], "source_url": "https://reactome.org/content/detail/R-HSA-5676637", "summary": "PTPLs dehydrate VLC3HA-CoA to VLCTDA-CoA is a Reactome release 97 curated reaction event. The source record connects VLC3HA-CoA to 2,3-TE-CoA, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:26527.", "equation": "VLC3HA-CoA → 2,3-TE-CoA + H2O", "inputs": [ { "node_id": "R-ALL-5676631", "reactome_id": "R-ALL-5676631", "label": "VLC3HA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57319", "formula": "C24H35N7O18P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5676634", "reactome_id": "R-ALL-5676634", "label": "2,3-TE-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58856", "formula": "C24H33N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-hydroxyacyl-CoA dehydratase activity of PTPLs [endoplasmic reticulum membrane]", "activity_id": "5676640", "ec_numbers": [ "4.2.1.150" ], "accessions": [] } ], "citations": [ { "id": "PMID:18554506", "title": "Characterization of four mammalian 3-hydroxyacyl-CoA dehydratases involved in very long-chain fatty acid synthesis", "journal": "FEBS Lett.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18554506/" } ], "x": 311.33333333333337, "y": 1688.4035087719299, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5678327", "label": "MIOX oxidises Ins to GlcA", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23697" ], "source_url": "https://reactome.org/content/detail/R-HSA-5678327", "summary": "MIOX oxidises Ins to GlcA is a Reactome release 97 curated reaction event. The source record connects Ins, O2 to GlcA, H2O, H+ in cytosol and cross-references RHEA:23697.", "equation": "Ins + O2 → GlcA + H2O + H+", "inputs": [ { "node_id": "R-ALL-426910", "reactome_id": "R-ALL-426910", "label": "Ins", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17268", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2162232", "reactome_id": "R-ALL-2162232", "label": "GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58720", "formula": "C6H9O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "inositol oxygenase activity of MIOX:2xFe2+ [cytosol]", "activity_id": "5678324", "ec_numbers": [ "1.13.99.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:15504367", "title": "Molecular cloning, expression, and characterization of myo-inositol oxygenase from mouse, rat, and human kidney", "journal": "Biochem. Biophys. Res. Commun.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15504367/" }, { "id": "PMID:18364358", "title": "Structural and biophysical characterization of human myo-inositol oxygenase", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18364358/" } ], "x": 7740, "y": 912.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5679041", "label": "ABCC2 transports BMG,BDG from cytosol to extracellular region", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · extracellular region · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5679041", "summary": "ABCC2 transports BMG,BDG from cytosol to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects BMG,BDG, ATP, H2O to BMG,BDG, ADP, Pi in cytosol · extracellular region · plasma membrane.", "equation": "BMG,BDG + ATP + H2O → BMG,BDG + ADP + Pi", "inputs": [ { "node_id": "R-ALL-5679042", "reactome_id": "R-ALL-5679042", "label": "BMG,BDG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5679034", "reactome_id": "R-ALL-5679034", "label": "BMG,BDG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "bilirubin transmembrane transporter activity of ABCC2 [plasma membrane]", "activity_id": "5679028", "ec_numbers": null, "accessions": [ "Q92887" ] } ], "citations": [ { "id": "PMID:10421658", "title": "Transport of monoglucuronosyl and bisglucuronosyl bilirubin by recombinant human and rat multidrug resistance protein 2", "journal": "Hepatology", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10421658/" } ], "x": 6521.333333333333, "y": 3011.3928571428573, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-5689000", "label": "AADAC deacetylates PHEN", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5689000", "summary": "AADAC deacetylates PHEN is a Reactome release 97 curated reaction event. The source record connects PHEN, H2O to 4-ethoxyaniline, acetyl group in endoplasmic reticulum membrane · cytosol.", "equation": "PHEN + H2O → 4-ethoxyaniline + acetyl group", "inputs": [ { "node_id": "R-ALL-5689006", "reactome_id": "R-ALL-5689006", "label": "PHEN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:8050", "formula": "C10H13NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5688995", "reactome_id": "R-ALL-5688995", "label": "4-ethoxyaniline", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:85505", "formula": "C8H11NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5689001", "reactome_id": "R-ALL-5689001", "label": "acetyl group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:40574", "formula": "C2H3O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "deacetylase activity of AADAC [endoplasmic reticulum membrane]", "activity_id": "5688996", "ec_numbers": null, "accessions": [ "P22760" ] } ], "citations": [ { "id": "PMID:20542992", "title": "Arylacetamide deacetylase is a determinant enzyme for the difference in hydrolase activities of phenacetin and acetaminophen", "journal": "Drug Metab. Dispos.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20542992/" }, { "id": "PMID:22813719", "title": "The emerging role of human esterases", "journal": "Drug Metab. Pharmacokinet.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22813719/" } ], "x": 8018.666666666667, "y": 3089.029411764706, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5690023", "label": "NUBPL binds 4Fe-4S", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5690023", "summary": "NUBPL binds 4Fe-4S is a Reactome release 97 curated reaction event. The source record connects NUBPL, 4Fe-4S to NUBPL:4Fe-4S in mitochondrial matrix.", "equation": "NUBPL + 4Fe-4S → NUBPL:4Fe-4S", "inputs": [ { "node_id": "R-HSA-5689133", "reactome_id": "R-HSA-5689133", "label": "NUBPL", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-169274", "reactome_id": "R-ALL-169274", "label": "4Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5690007", "reactome_id": "R-HSA-5690007", "label": "NUBPL:4Fe-4S", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19752196", "title": "Human ind1, an iron-sulfur cluster assembly factor for respiratory complex I", "journal": "Mol. Cell. Biol.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19752196/" }, { "id": "PMID:23553477", "title": "NUBPL mutations in patients with complex I deficiency and a distinct MRI pattern", "journal": "Neurology", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23553477/" } ], "x": 4451.333333333333, "y": 910.5666666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5690042", "label": "Peroxisomal ACOT4,6,8 hydrolyse MCFA-CoA, LCFA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:67681" ], "source_url": "https://reactome.org/content/detail/R-HSA-5690042", "summary": "Peroxisomal ACOT4,6,8 hydrolyse MCFA-CoA, LCFA-CoA is a Reactome release 97 curated reaction event. The source record connects MCFA-CoA, LCFA-CoA, H2O to MCFA, LCFA, CoA-SH, H+ in peroxisomal matrix and cross-references RHEA:67681.", "equation": "MCFA-CoA, LCFA-CoA + H2O → MCFA, LCFA + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-5690520", "reactome_id": "R-ALL-5690520", "label": "MCFA-CoA, LCFA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5690539", "reactome_id": "R-ALL-5690539", "label": "MCFA, LCFA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acyl-CoA hydrolase activity of ACOT4,8 [peroxisomal matrix]", "activity_id": "5690056", "ec_numbers": [ "3.1.2.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:16940157", "title": "Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs", "journal": "FASEB J.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16940157/" }, { "id": "PMID:11673457", "title": "Characterization of an acyl-coA thioesterase that functions as a major regulator of peroxisomal lipid metabolism", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11673457/" } ], "x": 590, "y": 1688.4035087719299, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5690043", "label": "Cytosolic ACOTs hydrolyse MCFA-CoA, LCFA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:68185", "RHEA:67681" ], "source_url": "https://reactome.org/content/detail/R-HSA-5690043", "summary": "Cytosolic ACOTs hydrolyse MCFA-CoA, LCFA-CoA is a Reactome release 97 curated reaction event. The source record connects MCFA-CoA, LCFA-CoA, H2O to MCFA, LCFA, CoA-SH, H+ in cytosol and cross-references RHEA:68185, RHEA:67681.", "equation": "MCFA-CoA, LCFA-CoA + H2O → MCFA, LCFA + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-5690044", "reactome_id": "R-ALL-5690044", "label": "MCFA-CoA, LCFA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5690064", "reactome_id": "R-ALL-5690064", "label": "MCFA, LCFA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acyl-CoA hydrolase activity of Cytosolic ACOTs, THEM4 [cytosol]", "activity_id": "5690067", "ec_numbers": [ "3.1.2.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:23700546", "title": "New players on the metabolic stage: How do you like Them Acots?", "journal": "Adipocyte", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23700546/" }, { "id": "PMID:22465940", "title": "The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22465940/" }, { "id": "PMID:20470824", "title": "Functional and structural properties of mammalian acyl-coenzyme A thioesterases", "journal": "Prog. Lipid Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20470824/" }, { "id": "PMID:16940157", "title": "Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs", "journal": "FASEB J.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16940157/" }, { "id": "PMID:25002576", "title": "Structural basis for regulation of the human acetyl-CoA thioesterase 12 and interactions with the steroidogenic acute regulatory protein-related lipid transfer (START) domain", "journal": "J. Biol. Chem.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25002576/" }, { "id": "PMID:16934754", "title": "Crystal structure of human thioesterase superfamily member 2", "journal": "Biochem. Biophys. Res. Commun.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16934754/" } ], "x": 868.6666666666667, "y": 1609.9473684210527, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5690046", "label": "PPT2 hydrolyses PALMCoA to PALM", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [ "RHEA:16646" ], "source_url": "https://reactome.org/content/detail/R-HSA-5690046", "summary": "PPT2 hydrolyses PALMCoA to PALM is a Reactome release 97 curated reaction event. The source record connects PALM-CoA, H2O to PALM, CoA-SH, H+ in lysosomal lumen and cross-references RHEA:16646.", "equation": "PALM-CoA + H2O → PALM + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-5690537", "reactome_id": "R-ALL-5690537", "label": "PALM-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57379", "formula": "C37H62N7O17P3S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5690516", "reactome_id": "R-ALL-5690516", "label": "PALM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:7896", "formula": "C16H31O2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1678675", "reactome_id": "R-ALL-1678675", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428040", "reactome_id": "R-ALL-428040", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "palmitoyl hydrolase activity of PPT2 [lysosomal lumen]", "activity_id": "5690062", "ec_numbers": null, "accessions": [ "Q9UMR5" ] } ], "citations": [ { "id": "PMID:23700546", "title": "New players on the metabolic stage: How do you like Them Acots?", "journal": "Adipocyte", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23700546/" }, { "id": "PMID:22465940", "title": "The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22465940/" }, { "id": "PMID:20470824", "title": "Functional and structural properties of mammalian acyl-coenzyme A thioesterases", "journal": "Prog. Lipid Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20470824/" }, { "id": "PMID:9341199", "title": "Molecular cloning and expression of palmitoyl-protein thioesterase 2 (PPT2), a homolog of lysosomal palmitoyl-protein thioesterase with a distinct substrate specificity", "journal": "J. Biol. Chem.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9341199/" } ], "x": 311.33333333333337, "y": 1673.3157894736842, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5690066", "label": "ACOT2,9,THEM4,5 hydrolyse MCFA-CoA, LCFA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:68185", "RHEA:67681" ], "source_url": "https://reactome.org/content/detail/R-HSA-5690066", "summary": "ACOT2,9,THEM4,5 hydrolyse MCFA-CoA, LCFA-CoA is a Reactome release 97 curated reaction event. The source record connects MCFA-CoA, LCFA-CoA, H2O to MCFA, LCFA, CoA-SH, H+ in mitochondrial matrix and cross-references RHEA:68185, RHEA:67681.", "equation": "MCFA-CoA, LCFA-CoA + H2O → MCFA, LCFA + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-5690540", "reactome_id": "R-ALL-5690540", "label": "MCFA-CoA, LCFA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5690519", "reactome_id": "R-ALL-5690519", "label": "MCFA, LCFA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acyl-CoA hydrolase activity of ACOT2,9, THEM4,5 dimer [mitochondrial matrix]", "activity_id": "5690041", "ec_numbers": [ "3.1.2.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:23700546", "title": "New players on the metabolic stage: How do you like Them Acots?", "journal": "Adipocyte", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23700546/" }, { "id": "PMID:22465940", "title": "The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22465940/" }, { "id": "PMID:16940157", "title": "Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs", "journal": "FASEB J.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16940157/" }, { "id": "PMID:22871024", "title": "Correlation of structure and function in the human hotdog-fold enzyme hTHEM4", "journal": "Biochemistry", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22871024/" }, { "id": "PMID:20470824", "title": "Functional and structural properties of mammalian acyl-coenzyme A thioesterases", "journal": "Prog. Lipid Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20470824/" }, { "id": "PMID:22586271", "title": "Acyl coenzyme A thioesterase Them5/Acot15 is involved in cardiolipin remodeling and fatty liver development", "journal": "Mol. Cell. Biol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22586271/" } ], "x": 311.33333333333337, "y": 1612.9649122807018, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5690517", "label": "PPT1 hydrolyses palmitoylated proteins", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [ "RHEA:19234" ], "source_url": "https://reactome.org/content/detail/R-HSA-5690517", "summary": "PPT1 hydrolyses palmitoylated proteins is a Reactome release 97 curated reaction event. The source record connects PALM:protein, H2O to PALM, protein, H+ in lysosomal lumen and cross-references RHEA:19234.", "equation": "PALM:protein + H2O → PALM + protein + H+", "inputs": [ { "node_id": "R-ALL-5690533", "reactome_id": "R-ALL-5690533", "label": "PALM:protein", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5690516", "reactome_id": "R-ALL-5690516", "label": "PALM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:7896", "formula": "C16H31O2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-6785219", "reactome_id": "R-ALL-6785219", "label": "protein", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36080", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-428040", "reactome_id": "R-ALL-428040", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "palmitoyl-(protein) hydrolase activity of PPT1 [lysosomal lumen]", "activity_id": "5690547", "ec_numbers": [ "3.1.2.22" ], "accessions": [ "P50897" ] } ], "citations": [ { "id": "PMID:23700546", "title": "New players on the metabolic stage: How do you like Them Acots?", "journal": "Adipocyte", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23700546/" }, { "id": "PMID:22465940", "title": "The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22465940/" }, { "id": "PMID:20470824", "title": "Functional and structural properties of mammalian acyl-coenzyme A thioesterases", "journal": "Prog. Lipid Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20470824/" }, { "id": "PMID:7901201", "title": "Purification and properties of a palmitoyl-protein thioesterase that cleaves palmitate from H-Ras", "journal": "J. Biol. Chem.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7901201/" }, { "id": "PMID:7916016", "title": "Molecular cloning and expression of palmitoyl-protein thioesterase", "journal": "J. Biol. Chem.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7916016/" } ], "x": 868.6666666666667, "y": 1679.3508771929824, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5690565", "label": "SCD desaturates ST-CoA to OLE-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:19722" ], "source_url": "https://reactome.org/content/detail/R-HSA-5690565", "summary": "SCD desaturates ST-CoA to OLE-CoA is a Reactome release 97 curated reaction event. The source record connects ST-CoA, H+, O2 to OLE-CoA, Fe(3+)Cb5, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:19722.", "equation": "ST-CoA + 2 H+ + O2 + 2 Fe(2+)Cb5 → OLE-CoA + 2 Fe(3+)Cb5 + 2 H2O", "inputs": [ { "node_id": "R-ALL-428139", "reactome_id": "R-ALL-428139", "label": "ST-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57394", "formula": "C39H66N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-4085415", "reactome_id": "R-ALL-4085415", "label": "Fe(2+)Cb5", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16518", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5690558", "reactome_id": "R-ALL-5690558", "label": "OLE-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57387", "formula": "C39H64N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-4085425", "reactome_id": "R-ALL-4085425", "label": "Fe(3+)Cb5", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18097", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "stearoyl-CoA 9-desaturase activity of SCD dimer [endoplasmic reticulum membrane]", "activity_id": "5690564", "ec_numbers": [ "1.14.19.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:7909540", "title": "Partial characterization of a cDNA for human stearoyl-CoA desaturase and changes in its mRNA expression in some normal and malignant tissues", "journal": "Int J Cancer", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7909540/" }, { "id": "PMID:10229681", "title": "Human stearoyl-CoA desaturase: alternative transcripts generated from a single gene by usage of tandem polyadenylation sites", "journal": "Biochem. J.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10229681/" }, { "id": "PMID:15610069", "title": "Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells", "journal": "Biochem. J.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15610069/" } ], "x": 590, "y": 1655.2105263157894, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5690911", "label": "ACO1:4Fe-4S isomerises CIT to ISCIT", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10337" ], "source_url": "https://reactome.org/content/detail/R-HSA-5690911", "summary": "ACO1:4Fe-4S isomerises CIT to ISCIT is a Reactome release 97 curated reaction event. The source record connects CIT to ISCIT in cytosol and cross-references RHEA:10337.", "equation": "CIT → ISCIT", "inputs": [ { "node_id": "R-ALL-76190", "reactome_id": "R-ALL-76190", "label": "CIT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16947", "formula": "C6H5O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389545", "reactome_id": "R-ALL-389545", "label": "ISCIT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15562", "formula": "C6H5O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aconitate hydratase activity of ACO1:4Fe-4S [cytosol]", "activity_id": "5690874", "ec_numbers": [ "4.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:1946430", "title": "A regulated RNA binding protein also possesses aconitase activity", "journal": "Proc. Natl. Acad. Sci. 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U.S.A.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8041788/" }, { "id": "PMID:16407072", "title": "Crystal structure of human iron regulatory protein 1 as cytosolic aconitase", "journal": "Structure", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16407072/" }, { "id": "PMID:25550467", "title": "Cytosolic aconitase activity sustains adipogenic capacity of adipose tissue connecting iron metabolism and adipogenesis", "journal": "FASEB J", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25550467/" } ], "x": 4451.333333333333, "y": 3427.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5692232", "label": "AKR1A1 oxidises BaPtDHD to BaP-7,8-dione", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5692232", "summary": "AKR1A1 oxidises BaPtDHD to BaP-7,8-dione is a Reactome release 97 curated reaction event. The source record connects BaPtDHD, NADP+ to NADPH, BaP-7,8-dione in cytosol.", "equation": "BaPtDHD + NADP+ → NADPH + BaP-7,8-dione", "inputs": [ { "node_id": "R-ALL-5692212", "reactome_id": "R-ALL-5692212", "label": "BaPtDHD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:81626", "formula": "C20H14O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5692216", "reactome_id": "R-ALL-5692216", "label": "BaP-7,8-dione", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87752", "formula": "C20H10O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alcohol dehydrogenase (NADP+) activity of AKR1A1 [cytosol]", "activity_id": "5692220", "ec_numbers": [ "1.1.1.2" ], "accessions": [ "P14550" ] } ], "citations": [ { "id": "PMID:23162467", "title": "The Role of Human Aldo-Keto Reductases in the Metabolic Activation and Detoxication of Polycyclic Aromatic Hydrocarbons: Interconversion of PAH Catechols and PAH o-Quinones", "journal": "Front Pharmacol", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23162467/" } ], "x": 7461.333333333333, "y": 3096.656862745098, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5692237", "label": "ADH5 oxidises S-HMGSH to S-FGSH", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19986" ], "source_url": "https://reactome.org/content/detail/R-HSA-5692237", "summary": "ADH5 oxidises S-HMGSH to S-FGSH is a Reactome release 97 curated reaction event. The source record connects NAD+, S-HMGSH to S-FGSH, H+, NADH in cytosol and cross-references RHEA:19986.", "equation": "NAD+ + S-HMGSH → S-FGSH + H+ + NADH", "inputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5692221", "reactome_id": "R-ALL-5692221", "label": "S-HMGSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:48926", "formula": "C11H19N3O7S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5692249", "reactome_id": "R-ALL-5692249", "label": "S-FGSH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16225", "formula": "C11H17N3O7S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "S-(hydroxymethyl)glutathione dehydrogenase [NAD(P)+] activity of ADH5:2xZn2+ dimer [cytosol]", "activity_id": "5692233", "ec_numbers": [ "1.1.1.284" ], "accessions": [] } ], "citations": [ { "id": "PMID:3365377", "title": "Class III human liver alcohol dehydrogenase: a novel structural type equidistantly related to the class I and class II enzymes", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3365377/" }, { "id": "PMID:3278908", "title": "Rat liver alcohol dehydrogenase of class III. Primary structure, functional consequences and relationships to other alcohol dehydrogenases", "journal": "Eur. J. Biochem.", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3278908/" } ], "x": 7740, "y": 2860.205882352941, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5692261", "label": "ALDH3A2-1 oxidises HD2NAL to PALM", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5692261", "summary": "ALDH3A2-1 oxidises HD2NAL to PALM is a Reactome release 97 curated reaction event. The source record connects H2O, HD2NAL, NAD+ to H+, NADH, PALM in cytosol · endoplasmic reticulum membrane.", "equation": "H2O + HD2NAL + NAD+ → H+ + NADH + PALM", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428671", "reactome_id": "R-ALL-428671", "label": "HD2NAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17585", "formula": "C16H30O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-3632881", "reactome_id": "R-ALL-3632881", "label": "PALM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:7896", "formula": "C16H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of ALDH3A2-1 dimer [endoplasmic reticulum membrane]", "activity_id": "5692275", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:17510064", "title": "Dual subcellular localization in the endoplasmic reticulum and peroxisomes and a vital role in protecting against oxidative stress of fatty aldehyde dehydrogenase are achieved by alternative splicing", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17510064/" }, { "id": "PMID:8528251", "title": "Sjögren-Larsson syndrome is caused by mutations in the fatty aldehyde dehydrogenase gene", "journal": "Nat. Genet.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8528251/" }, { "id": "PMID:19965611", "title": "Monitoring of fatty aldehyde dehydrogenase by formation of pyrenedecanoic acid from pyrenedecanal", "journal": "J. Lipid Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19965611/" }, { "id": "PMID:9133646", "title": "Human liver fatty aldehyde dehydrogenase: microsomal localization, purification, and biochemical characterization", "journal": "Biochim. Biophys. Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9133646/" }, { "id": "PMID:22633490", "title": "The Sjögren-Larsson syndrome gene encodes a hexadecenal dehydrogenase of the sphingosine 1-phosphate degradation pathway", "journal": "Mol. Cell", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22633490/" }, { "id": "PMID:11306053", "title": "Fatty aldehyde dehydrogenase: genomic structure, expression and mutation analysis in Sjögren-Larsson syndrome", "journal": "Chem. Biol. Interact.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11306053/" } ], "x": 3321.3333333333335, "y": 1675.1346153846155, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5692283", "label": "ALD3A1 oxidises 4HPCP to CXPA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5692283", "summary": "ALD3A1 oxidises 4HPCP to CXPA is a Reactome release 97 curated reaction event. The source record connects 4HPCP, H2O, NADP+ to CXPA, H+, NADPH in cytosol.", "equation": "4HPCP + H2O + NADP+ → CXPA + H+ + NADPH", "inputs": [ { "node_id": "R-ALL-5692409", "reactome_id": "R-ALL-5692409", "label": "4HPCP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:196991", "formula": "C7H15Cl2N2O4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5692433", "reactome_id": "R-ALL-5692433", "label": "CXPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:3410", "formula": "C7H15Cl2N2O4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldehyde dehydrogenase [NAD(P)+] activity of ALDH3A1 [cytosol]", "activity_id": "5692290", "ec_numbers": [ "1.2.1.5" ], "accessions": [ "P30838" ] } ], "citations": [ { "id": "PMID:16614850", "title": "RNAi-mediated knockdown of aldehyde dehydrogenase class-1A1 and class-3A1 is specific and reveals that each contributes equally to the resistance against 4-hydroperoxycyclophosphamide", "journal": "Cancer Chemother. 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The source record connects BCKDH, ATP to p-BCKDH, ADP, H+ in mitochondrial matrix and cross-references RHEA:17302.", "equation": "BCKDH + 2 ATP → p-BCKDH + 2 ADP + H+", "inputs": [ { "node_id": "R-HSA-70019", "reactome_id": "R-HSA-70019", "label": "BCKDH", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-5693120", "reactome_id": "R-HSA-5693120", "label": "p-BCKDH", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein kinase activity of BCKDK dimer [mitochondrial matrix]", "activity_id": "5693164", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15166214", "title": "Cross-talk between thiamin diphosphate binding and phosphorylation loop conformation in human branched-chain alpha-keto acid decarboxylase/dehydrogenase", "journal": "J. 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Mutat.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24449431/" } ], "x": 6800, "y": 1657.5833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693153", "label": "PPM1K dephosphorylates p-BCKDH", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:77248" ], "source_url": "https://reactome.org/content/detail/R-HSA-5693153", "summary": "PPM1K dephosphorylates p-BCKDH is a Reactome release 97 curated reaction event. The source record connects p-BCKDH, H2O to BCKDH, Pi in mitochondrial matrix and cross-references RHEA:77248.", "equation": "p-BCKDH + 2 H2O → BCKDH + 2 Pi", "inputs": [ { "node_id": "R-HSA-5693120", "reactome_id": "R-HSA-5693120", "label": "p-BCKDH", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-70019", "reactome_id": "R-HSA-70019", "label": "BCKDH", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "[3-methyl-2-oxobutanoate dehydrogenase (lipoamide)]-phosphatase activity of PPM1K:Mn2+ [mitochondrial matrix]", "activity_id": "5693125", "ec_numbers": [ "3.1.3.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:22291014", "title": "Structural and biochemical characterization of human mitochondrial branched-chain α-ketoacid dehydrogenase phosphatase", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22291014/" } ], "x": 6521.333333333333, "y": 1674.1944444444443, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693347", "label": "CRYM reduces P2C to PPCA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:12526" ], "source_url": "https://reactome.org/content/detail/R-HSA-5693347", "summary": "CRYM reduces P2C to PPCA is a Reactome release 97 curated reaction event. The source record connects P2C, H+, NADPH to PPCA, NADP+ in peroxisomal matrix and cross-references RHEA:12526.", "equation": "P2C + H+ + NADPH → PPCA + NADP+", "inputs": [ { "node_id": "R-ALL-5693311", "reactome_id": "R-ALL-5693311", "label": "P2C", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77631", "formula": "C6H9NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113602", "reactome_id": "R-ALL-113602", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5693325", "reactome_id": "R-ALL-5693325", "label": "PPCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61185", "formula": "C6H11NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389556", "reactome_id": "R-ALL-389556", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "delta1-piperideine-2-carboxylate reductase activity of CRYM [peroxisomal matrix]", "activity_id": "5693316", "ec_numbers": [ "1.5.1.21" ], "accessions": [ "Q14894" ] } ], "citations": [ { "id": "PMID:25931162", "title": "Insights into Enzyme Catalysis and Thyroid Hormone Regulation of Cerebral Ketimine Reductase/μ-Crystallin Under Physiological Conditions", "journal": "Neurochem. Res.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25931162/" }, { "id": "PMID:17242435", "title": "Crystal structure of human micro-crystallin complexed with NADPH", "journal": "Protein Sci.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17242435/" } ], "x": 6800, "y": 2049.375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693356", "label": "CEMIP hydrolyzes HA", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5693356", "summary": "CEMIP hydrolyzes HA is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects HA to HA, (HA)50 in extracellular region.", "equation": "HA → HA + (HA)50", "inputs": [ { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false }, { "node_id": "R-ALL-2160894", "reactome_id": "R-ALL-2160894", "label": "(HA)50", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Polymer", "currency": false } ], "catalysts": [ { "label": "hyalurononglucosaminidase activity of CEMIP [extracellular region]", "activity_id": "5693345", "ec_numbers": [ "3.2.1.35" ], "accessions": [ "Q8WUJ3" ] } ], "citations": [ { "id": "PMID:23509262", "title": "KIAA1199, a deafness gene of unknown function, is a new hyaluronan binding protein involved in hyaluronan depolymerization", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23509262/" }, { "id": "PMID:37953485", "title": "Hyaluronic acid metabolism and chemotherapy resistance: recent advances and therapeutic potential", "journal": "Mol Oncol", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/37953485/" } ], "x": 2938.666666666667, "y": 1042.590909090909, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-5693390", "label": "HSD17B11 dehydrogenates EST17b to E1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24617" ], "source_url": "https://reactome.org/content/detail/R-HSA-5693390", "summary": "HSD17B11 dehydrogenates EST17b to E1 is a Reactome release 97 curated reaction event. The source record connects EST17b, NADP+ to E1, H+, NADPH in cytosol and cross-references RHEA:24617.", "equation": "EST17b + NADP+ → E1 + H+ + NADPH", "inputs": [ { "node_id": "R-ALL-193132", "reactome_id": "R-ALL-193132", "label": "EST17b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16469", "formula": "C18H24O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193066", "reactome_id": "R-ALL-193066", "label": "E1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17263", "formula": "C18H22O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "estradiol 17-beta-dehydrogenase [NAD(P)+] activity of HSD17B11 [cytosol]", "activity_id": "5693396", "ec_numbers": [ "1.1.1.62" ], "accessions": [ "Q8NBQ5" ] } ], "citations": [ { "id": "PMID:12697717", "title": "17 beta-hydroxysteroid dehydrogenase type XI localizes to human steroidogenic cells", "journal": "Endocrinology", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12697717/" } ], "x": 4451.333333333333, "y": 1612.5681818181818, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693691", "label": "CES1 trimer.CES2 hydrolyse COCN to BEG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5693691", "summary": "CES1 trimer.CES2 hydrolyse COCN to BEG is a Reactome release 97 curated reaction event. The source record connects H2O, COCN to BEG, MeOH, H+ in endoplasmic reticulum lumen.", "equation": "H2O + COCN → BEG + MeOH + H+", "inputs": [ { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5693702", "reactome_id": "R-ALL-5693702", "label": "COCN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27958", "formula": "C17H21NO4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5693712", "reactome_id": "R-ALL-5693712", "label": "BEG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:41001", "formula": "C16H19NO4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5693711", "reactome_id": "R-ALL-5693711", "label": "MeOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17790", "formula": "CH4O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carboxylic ester hydrolase activity of CES1 trimer, CES2 [endoplasmic reticulum lumen]", "activity_id": "5693697", "ec_numbers": [ "3.1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:7980644", "title": "Purification and characterization of a human liver cocaine carboxylesterase that catalyzes the production of benzoylecgonine and the formation of cocaethylene from alcohol and cocaine", "journal": "Biochem. Pharmacol.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7980644/" }, { "id": "PMID:9169443", "title": "Purification and cloning of a broad substrate specificity human liver carboxylesterase that catalyzes the hydrolysis of cocaine and heroin", "journal": "J. Biol. Chem.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9169443/" }, { "id": "PMID:12725862", "title": "Crystal structure of human carboxylesterase 1 complexed with the Alzheimer's drug tacrine: from binding promiscuity to selective inhibition", "journal": "Chem. Biol.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12725862/" } ], "x": 8018.666666666667, "y": 3066.1470588235293, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693724", "label": "ESD dimer hydrolyses S-FGSH to GSH", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [ "RHEA:14962" ], "source_url": "https://reactome.org/content/detail/R-HSA-5693724", "summary": "ESD dimer hydrolyses S-FGSH to GSH is a Reactome release 97 curated reaction event. The source record connects S-FGSH, H2O to GSH, formate in endoplasmic reticulum lumen and cross-references RHEA:14962.", "equation": "S-FGSH + H2O → GSH + formate", "inputs": [ { "node_id": "R-ALL-5693723", "reactome_id": "R-ALL-5693723", "label": "S-FGSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16225", "formula": "C11H17N3O7S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-158543", "reactome_id": "R-ALL-158543", "label": "GSH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16856", "formula": "C10H17N3O6S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-194712", "reactome_id": "R-ALL-194712", "label": "formate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "S-formylglutathione hydrolase activity of ESD dimer [endoplasmic reticulum lumen]", "activity_id": "5693721", "ec_numbers": [ "3.1.2.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:4768551", "title": "Esterase D: a new human polymorphism", "journal": "Ann. Hum. Genet.", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4768551/" }, { "id": "PMID:3770744", "title": "Identity of the polymorphisms for esterase D and S-formylglutathione hydrolase in red blood cells", "journal": "Hum. Genet.", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3770744/" } ], "x": 8018.666666666667, "y": 2913.598039215686, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693742", "label": "FAAH hydrolyses AEA to AA and ETA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:26137" ], "source_url": "https://reactome.org/content/detail/R-HSA-5693742", "summary": "FAAH hydrolyses AEA to AA and ETA is a Reactome release 97 curated reaction event. The source record connects AEA, H2O to AA, ETA in endoplasmic reticulum membrane · cytosol and cross-references RHEA:26137.", "equation": "AEA + H2O → AA + ETA", "inputs": [ { "node_id": "R-ALL-5693754", "reactome_id": "R-ALL-5693754", "label": "AEA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2700", "formula": "C22H37NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1498751", "reactome_id": "R-ALL-1498751", "label": "ETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57603", "formula": "C2H8NO", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fatty acid amide hydrolase activity of FAAH [endoplasmic reticulum membrane]", "activity_id": "5693749", "ec_numbers": [ "3.5.1.99" ], "accessions": [ "O00519" ] } ], "citations": [ { "id": "PMID:17015445", "title": "A second fatty acid amide hydrolase with variable distribution among placental mammals", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17015445/" }, { "id": "PMID:19926788", "title": "Lipid droplets are novel sites of N-acylethanolamine inactivation by fatty acid amide hydrolase-2", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19926788/" } ], "x": 590, "y": 1585.8070175438597, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693751", "label": "FAAH2 hydrolyses AEA to AA and ETA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "lipid droplet", "compartment_ids": [ "GO:0005811" ], "rhea_ids": [ "RHEA:26137" ], "source_url": "https://reactome.org/content/detail/R-HSA-5693751", "summary": "FAAH2 hydrolyses AEA to AA and ETA is a Reactome release 97 curated reaction event. The source record connects AEA, H2O to AA, ETA in lipid droplet and cross-references RHEA:26137.", "equation": "AEA + H2O → AA + ETA", "inputs": [ { "node_id": "R-ALL-5693752", "reactome_id": "R-ALL-5693752", "label": "AEA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:2700", "formula": "C22H37NO2", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5693747", "reactome_id": "R-ALL-5693747", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5693733", "reactome_id": "R-ALL-5693733", "label": "AA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5693736", "reactome_id": "R-ALL-5693736", "label": "ETA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57603", "formula": "C2H8NO", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fatty acid amide hydrolase activity of FAAH2 [lipid droplet]", "activity_id": "5693738", "ec_numbers": [ "3.5.1.99" ], "accessions": [ "Q6GMR7" ] } ], "citations": [ { "id": "PMID:17015445", "title": "A second fatty acid amide hydrolase with variable distribution among placental mammals", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17015445/" }, { "id": "PMID:19926788", "title": "Lipid droplets are novel sites of N-acylethanolamine inactivation by fatty acid amide hydrolase-2", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19926788/" } ], "x": 868.6666666666667, "y": 1585.8070175438597, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693761", "label": "FA2H hydroxylates 1,2-saturated fatty acids", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5693761", "summary": "FA2H hydroxylates 1,2-saturated fatty acids is a Reactome release 97 curated reaction event. The source record connects O2, 2-saturated fatty acid, CYB5B:Fe2+ to H2O, 2-OH FA, CYB5B:Fe3+ in endoplasmic reticulum membrane · cytosol.", "equation": "O2 + 2-saturated fatty acid + 2 CYB5B:Fe2+ + 2 H+ → H2O + 2-OH FA + 2 CYB5B:Fe3+", "inputs": [ { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9843199", "reactome_id": "R-ALL-9843199", "label": "2-saturated fatty acid", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83955", "formula": "C2H2O2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9842697", "reactome_id": "R-HSA-9842697", "label": "CYB5B:Fe2+", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9843201", "reactome_id": "R-ALL-9843201", "label": "2-OH FA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76177", "formula": "C2H2O3R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9842694", "reactome_id": "R-HSA-9842694", "label": "CYB5B:Fe3+", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "fatty acid 2-hydroxylase activity of FA2H [endoplasmic reticulum membrane]", "activity_id": "5693796", "ec_numbers": null, "accessions": [ "Q7L5A8" ] } ], "citations": [ { "id": "PMID:15337768", "title": "The human FA2H gene encodes a fatty acid 2-hydroxylase", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15337768/" }, { "id": "PMID:17355976", "title": "Fatty acid 2-hydroxylase, encoded by FA2H, accounts for differentiation-associated increase in 2-OH ceramides during keratinocyte differentiation", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17355976/" }, { "id": "PMID:20026285", "title": "Fatty acid 2-Hydroxylation in mammalian sphingolipid biology", "journal": "Biochim Biophys Acta", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20026285/" }, { "id": "PMID:36902339", "title": "Fatty Acid 2-Hydroxylase and 2-Hydroxylated Sphingolipids: Metabolism and Function in Health and Diseases", "journal": "Int J Mol Sci", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36902339/" }, { "id": "PMID:22517924", "title": "Stereospecificity of fatty acid 2-hydroxylase and differential functions of 2-hydroxy fatty acid enantiomers", "journal": "J Lipid Res", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22517924/" } ], "x": 3600, "y": 1720.0192307692307, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693967", "label": "PXLP-K754-GLDC dimer decarboxylates Gly", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24305" ], "source_url": "https://reactome.org/content/detail/R-HSA-5693967", "summary": "PXLP-K754-GLDC dimer decarboxylates Gly is a Reactome release 97 curated reaction event. The source record connects Gly, Lipo-K107-GCSH, H+ to CO2, AmMeLipoGCSH:SAMDLL in mitochondrial matrix and cross-references RHEA:24305.", "equation": "Gly + Lipo-K107-GCSH + H+ → CO2 + AmMeLipoGCSH:SAMDLL", "inputs": [ { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-6793611", "reactome_id": "R-HSA-6793611", "label": "Lipo-K107-GCSH", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-5693969", "reactome_id": "R-HSA-5693969", "label": "AmMeLipoGCSH:SAMDLL", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "glycine dehydrogenase (decarboxylating) activity of PXLP-K754-GLDC dimer [mitochondrial matrix]", "activity_id": "5693965", "ec_numbers": [ "1.4.4.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:1993704", "title": "The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures", "journal": "J. Biol. Chem.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1993704/" }, { "id": "PMID:2025283", "title": "The primary structure of human H-protein of the glycine cleavage system deduced by cDNA cloning", "journal": "Biochem. Biophys. Res. Commun.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2025283/" }, { "id": "PMID:1959641", "title": "Lipoylation of H-protein of the glycine cleavage system. The effect of site-directed mutagenesis of amino acid residues around the lipoyllysine residue on the lipoate attachment", "journal": "FEBS Lett.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1959641/" } ], "x": 311.33333333333337, "y": 3302.5238095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5693977", "label": "AMT transfers NH2CH2 from GCSH:SAMDLL to THF", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:16946" ], "source_url": "https://reactome.org/content/detail/R-HSA-5693977", "summary": "AMT transfers NH2CH2 from GCSH:SAMDLL to THF is a Reactome release 97 curated reaction event. The source record connects AmMeLipoGCSH:SAMDLL, THF to GCSH:DHLL, 5,10-methylene-THF, NH4+ in mitochondrial matrix and cross-references RHEA:16946.", "equation": "AmMeLipoGCSH:SAMDLL + THF → GCSH:DHLL + 5,10-methylene-THF + NH4+", "inputs": [ { "node_id": "R-HSA-5693969", "reactome_id": "R-HSA-5693969", "label": "AmMeLipoGCSH:SAMDLL", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-200671", "reactome_id": "R-ALL-200671", "label": "THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5693982", "reactome_id": "R-HSA-5693982", "label": "GCSH:DHLL", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-5693984", "reactome_id": "R-ALL-5693984", "label": "5,10-methylene-THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15636", "formula": "C20H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113561", "reactome_id": "R-ALL-113561", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aminomethyltransferase activity of AMT [mitochondrial matrix]", "activity_id": "5693980", "ec_numbers": [ "2.1.2.10" ], "accessions": [ "P48728" ] } ], "citations": [ { "id": "PMID:6469978", "title": "Mechanism of the glycine cleavage reaction. Further characterization of the intermediate attached to H-protein and of the reaction catalyzed by T-protein", "journal": "J. Biol. Chem.", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6469978/" }, { "id": "PMID:16051266", "title": "Crystal structure of human T-protein of glycine cleavage system at 2.0 A resolution and its implication for understanding non-ketotic hyperglycinemia", "journal": "J Mol Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16051266/" } ], "x": 590, "y": 3302.5238095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5694018", "label": "DLD dimer:2xFAD oxidises GCSH:DHLL to GCSH:lipoate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5694018", "summary": "DLD dimer:2xFAD oxidises GCSH:DHLL to GCSH:lipoate is a Reactome release 97 curated reaction event. The source record connects GCSH:DHLL, NAD+ to Lipo-K107-GCSH, H+, NADH in mitochondrial matrix.", "equation": "GCSH:DHLL + NAD+ → Lipo-K107-GCSH + H+ + NADH", "inputs": [ { "node_id": "R-HSA-5693982", "reactome_id": "R-HSA-5693982", "label": "GCSH:DHLL", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-6793611", "reactome_id": "R-HSA-6793611", "label": "Lipo-K107-GCSH", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dihydrolipoyl dehydrogenase (NADH) activity of OGDH complex [mitochondrial matrix]", "activity_id": "5694028", "ec_numbers": [ "1.8.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:9242632", "title": "Dihydrolipoamide dehydrogenase-binding protein of the human pyruvate dehydrogenase complex. DNA-derived amino acid sequence, expression, and reconstitution of the pyruvate dehydrogenase complex.", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9242632/" }, { "id": "PMID:16263718", "title": "How dihydrolipoamide dehydrogenase-binding protein binds dihydrolipoamide dehydrogenase in the human pyruvate dehydrogenase complex", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16263718/" } ], "x": 868.6666666666667, "y": 3302.5238095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5694071", "label": "GLO1 dimer:2xZn2+ transforms MGXL and GSH to (R)-S-LGSH", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19071" ], "source_url": "https://reactome.org/content/detail/R-HSA-5694071", "summary": "GLO1 dimer:2xZn2+ transforms MGXL and GSH to (R)-S-LGSH is a Reactome release 97 curated reaction event. The source record connects GSH, MGXL to (R)-S-LGSH in cytosol and cross-references RHEA:19071.", "equation": "GSH + MGXL → (R)-S-LGSH", "inputs": [ { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5694074", "reactome_id": "R-ALL-5694074", "label": "MGXL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17158", "formula": "C3H4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5694130", "reactome_id": "R-ALL-5694130", "label": "(R)-S-LGSH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57474", "formula": "C13H20N3O8S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lactoylglutathione lyase activity of GLO1 dimer:2xZn2+ [cytosol]", "activity_id": "5694145", "ec_numbers": [ "4.4.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:9705294", "title": "Involvement of an active-site Zn2+ ligand in the catalytic mechanism of human glyoxalase I", "journal": "J. Biol. Chem.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9705294/" } ], "x": 5008.666666666667, "y": 438.04999999999995, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5694077", "label": "EPHX1 hydrates BaP4,5O to BaP4,5-DHD", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5694077", "summary": "EPHX1 hydrates BaP4,5O to BaP4,5-DHD is a Reactome release 97 curated reaction event. The source record connects BaP4,5O, H2O to BaP4,5-DHD in endoplasmic reticulum membrane · cytosol.", "equation": "BaP4,5O + H2O → BaP4,5-DHD", "inputs": [ { "node_id": "R-ALL-5694080", "reactome_id": "R-ALL-5694080", "label": "BaP4,5O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:34560", "formula": "C20H12O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5694091", "reactome_id": "R-ALL-5694091", "label": "BaP4,5-DHD", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:81621", "formula": "C20H14O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cis-stilbene-oxide hydrolase activity of EPHX1 [endoplasmic reticulum membrane]", "activity_id": "5694072", "ec_numbers": [ "3.3.2.9" ], "accessions": [ "P07099" ] } ], "citations": [ { "id": "PMID:15535985", "title": "Functional analysis of human microsomal epoxide hydrolase genetic variants", "journal": "Chem. Biol. Interact.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15535985/" }, { "id": "PMID:11154734", "title": "Epoxide hydrolases: biochemistry and molecular biology", "journal": "Chem. Biol. Interact.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11154734/" } ], "x": 8018.666666666667, "y": 3111.9117647058824, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5694137", "label": "PXLP-K280-SHMT2 tetramer transfers CH2OH group to Gly to form L-Ser", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:15482" ], "source_url": "https://reactome.org/content/detail/R-HSA-5694137", "summary": "PXLP-K280-SHMT2 tetramer transfers CH2OH group to Gly to form L-Ser is a Reactome release 97 curated reaction event. The source record connects 5,10-methylene-THF, Gly, H2O to THF, L-Ser in mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:15482.", "equation": "5,10-methylene-THF + Gly + H2O → THF + L-Ser", "inputs": [ { "node_id": "R-ALL-5693984", "reactome_id": "R-ALL-5693984", "label": "5,10-methylene-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15636", "formula": "C20H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-200671", "reactome_id": "R-ALL-200671", "label": "THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-379732", "reactome_id": "R-ALL-379732", "label": "L-Ser", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycine hydroxymethyltransferase activity of PXLP-K280-SHMT2 tetramer [mitochondrial inner membrane]", "activity_id": "5694152", "ec_numbers": [ "2.1.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:21876188", "title": "Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21876188/" } ], "x": 5008.666666666667, "y": 2820.681818181818, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5694485", "label": "ABHD3 hydrolyses LPC(14:0) to 1AGPC", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5694485", "summary": "ABHD3 hydrolyses LPC(14:0) to 1AGPC is a Reactome release 97 curated reaction event. The source record connects LPC(14:0), H2O to 1AGPC, RCOO- in plasma membrane · cytosol.", "equation": "LPC(14:0) + H2O → 1AGPC + RCOO-", "inputs": [ { "node_id": "R-ALL-5694502", "reactome_id": "R-ALL-5694502", "label": "LPC(14:0)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64483", "formula": "C22H46NO7P", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5694457", "reactome_id": "R-ALL-5694457", "label": "1AGPC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11230", "formula": "C9H20NO7PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1861754", "reactome_id": "R-ALL-1861754", "label": "RCOO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28868", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of ABHD3 [plasma membrane]", "activity_id": "5694461", "ec_numbers": [ "3.1.1.32" ], "accessions": [ "Q8WU67" ] } ], "citations": [ { "id": "PMID:21926997", "title": "Metabolomics annotates ABHD3 as a physiologic regulator of medium-chain phospholipids", "journal": "Nat. Chem. Biol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21926997/" }, { "id": "PMID:23328280", "title": "Mammalian alpha beta hydrolase domain (ABHD) proteins: Lipid metabolizing enzymes at the interface of cell signaling and energy metabolism", "journal": "Biochim. Biophys. Acta", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23328280/" } ], "x": 2660, "y": 1812.7602739726028, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5694494", "label": "AACS ligates CoA-SH to ACA, forming ACA-CoA", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16118" ], "source_url": "https://reactome.org/content/detail/R-HSA-5694494", "summary": "AACS ligates CoA-SH to ACA, forming ACA-CoA is a Reactome release 97 curated reaction event. The source record connects ACA, ATP, CoA-SH to ACA-CoA, AMP, PPi in cytosol and cross-references RHEA:16118.", "equation": "ACA + ATP + CoA-SH → ACA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-29666", "reactome_id": "R-ALL-29666", "label": "ACA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:13705", "formula": "C4H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-191293", "reactome_id": "R-ALL-191293", "label": "ACA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57286", "formula": "C25H36N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetoacetate-CoA ligase activity of AACS [cytosol]", "activity_id": "5694501", "ec_numbers": [ "6.2.1.16" ], "accessions": [ "Q86V21" ] } ], "citations": [ { "id": "PMID:20102333", "title": "Transcriptional regulation of the human acetoacetyl-CoA synthetase gene by PPARgamma", "journal": "Biochem. 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Physiol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23396451/" } ], "x": 7461.333333333333, "y": 311.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5694563", "label": "ABHD10 hydrolyses MPAG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5694563", "summary": "ABHD10 hydrolyses MPAG is a Reactome release 97 curated reaction event. The source record connects AMPAG, H2O to MPA, GlcA in mitochondrial matrix.", "equation": "AMPAG + H2O → MPA + GlcA", "inputs": [ { "node_id": "R-ALL-5694574", "reactome_id": "R-ALL-5694574", "label": "AMPAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64689", "formula": "C23H28O12", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5694565", "reactome_id": "R-ALL-5694565", "label": "MPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62932", "formula": "C17H19O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5694569", "reactome_id": "R-ALL-5694569", "label": "GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15748", "formula": "C6H9O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity, hydrolyzing O-glycosyl compounds of ABHD10 [mitochondrial matrix]", "activity_id": "5694578", "ec_numbers": [ "3.2.1" ], "accessions": [ "Q9NUJ1" ] } ], "citations": [ { "id": "PMID:22294686", "title": "Human α/β hydrolase domain containing 10 (ABHD10) is responsible enzyme for deglucuronidation of mycophenolic acid acyl-glucuronide in liver", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22294686/" } ], "x": 7740, "y": 3096.656862745098, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5694583", "label": "ABHD4 hydrolyses NAPE", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5694583", "summary": "ABHD4 hydrolyses NAPE is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects NAPE, H2O to GPAEA, acyl group in cytosol · endoplasmic reticulum membrane.", "equation": "NAPE + H2O → GPAEA + acyl group", "inputs": [ { "node_id": "R-ALL-5694568", "reactome_id": "R-ALL-5694568", "label": "NAPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61232", "formula": "C8H11NO9PR3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5694580", "reactome_id": "R-ALL-5694580", "label": "GPAEA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:52571", "formula": "C27H42NO9PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5694567", "reactome_id": "R-ALL-5694567", "label": "acyl group", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:22221", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity of ABHD4 [endoplasmic reticulum membrane]", "activity_id": "5694572", "ec_numbers": [ "3" ], "accessions": [ "Q8TB40" ] } ], "citations": [ { "id": "PMID:16818490", "title": "Endocannabinoid biosynthesis proceeding through glycerophospho-N-acyl ethanolamine and a role for alpha/beta-hydrolase 4 in this pathway", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16818490/" } ], "x": 2938.666666666667, "y": 1812.7602739726028, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-5695957", "label": "ACSBG1,2 ligates CoA-SH to VLCFA, forming VLCFA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:54537" ], "source_url": "https://reactome.org/content/detail/R-HSA-5695957", "summary": "ACSBG1,2 ligates CoA-SH to VLCFA, forming VLCFA-CoA is a Reactome release 97 curated reaction event. The source record connects VLCFA, ATP, CoA-SH to VLCFA-CoA, AMP, PPi in cytosol and cross-references RHEA:54537.", "equation": "VLCFA + ATP + CoA-SH → VLCFA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-5695985", "reactome_id": "R-ALL-5695985", "label": "VLCFA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58950", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5695971", "reactome_id": "R-ALL-5695971", "label": "VLCFA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:138261", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "very long-chain fatty acid-CoA ligase activity of ACSBG1,2 [cytosol]", "activity_id": "5695982", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10954726", "title": "Very long-chain acyl-CoA synthetases. Human \"bubblegum\" represents a new family of proteins capable of activating very long-chain fatty acids", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10954726/" }, { "id": "PMID:12975357", "title": "The acyl-CoA synthetase \"bubblegum\" (lipidosin): further characterization and role in neuronal fatty acid beta-oxidation.", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12975357/" }, { "id": "PMID:16371355", "title": "The second member of the human and murine bubblegum family is a testis- and brainstem-specific acyl-CoA synthetase", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16371355/" } ], "x": 311.33333333333337, "y": 2286.333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5695964", "label": "ABHD14B hydrolyses PNPB", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5695964", "summary": "ABHD14B hydrolyses PNPB is a Reactome release 97 curated reaction event. The source record connects PNPB, H2O to PNP, BUT in cytosol.", "equation": "PNPB + H2O → PNP + BUT", "inputs": [ { "node_id": "R-ALL-5695960", "reactome_id": "R-ALL-5695960", "label": "PNPB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:85867", "formula": "C10H11NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5695950", "reactome_id": "R-ALL-5695950", "label": "PNP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16836", "formula": "C6H5NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76179", "reactome_id": "R-ALL-76179", "label": "BUT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17968", "formula": "C4H7O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity of ABHD14B [cytosol]", "activity_id": "5695998", "ec_numbers": [ "3" ], "accessions": [ "Q96IU4" ] } ], "citations": [ { "id": "PMID:14672934", "title": "The crystal structure of CCG1/TAF(II)250-interacting factor B (CIB)", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14672934/" } ], "x": 8018.666666666667, "y": 3096.656862745098, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5695980", "label": "ACAD10 dehydrogenates S-2MPDA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5695980", "summary": "ACAD10 dehydrogenates S-2MPDA-CoA is a Reactome release 97 curated reaction event. The source record connects S-2MPDA-CoA, FAD to S-2MDPDA-CoA, FADH2 in mitochondrial matrix.", "equation": "S-2MPDA-CoA + FAD → S-2MDPDA-CoA + FADH2", "inputs": [ { "node_id": "R-ALL-5695946", "reactome_id": "R-ALL-5695946", "label": "S-2MPDA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:85944", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5695949", "reactome_id": "R-ALL-5695949", "label": "S-2MDPDA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:85945", "formula": "C37H64N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACAD10 [mitochondrial matrix]", "activity_id": "5695993", "ec_numbers": [ "1.3.99.3" ], "accessions": [ "Q6JQN1" ] } ], "citations": [ { "id": "PMID:21237683", "title": "Identification and characterization of new long chain acyl-CoA dehydrogenases", "journal": "Mol. Genet. Metab.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21237683/" } ], "x": 590, "y": 1715.561403508772, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5695989", "label": "ACAD11 dehydrogenates BH-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5695989", "summary": "ACAD11 dehydrogenates BH-CoA is a Reactome release 97 curated reaction event. The source record connects BH-CoA, FAD to DHB-CoA, FADH2 in mitochondrial matrix · mitochondrial inner membrane.", "equation": "BH-CoA + FAD → DHB-CoA + FADH2", "inputs": [ { "node_id": "R-ALL-5695963", "reactome_id": "R-ALL-5695963", "label": "BH-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65059", "formula": "C43H74N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5695976", "reactome_id": "R-ALL-5695976", "label": "DHB-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:85880", "formula": "C43H76N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACAD11 [mitochondrial inner membrane]", "activity_id": "5695961", "ec_numbers": [ "1.3.99.3" ], "accessions": [ "Q709F0" ] } ], "citations": [ { "id": "PMID:21237683", "title": "Identification and characterization of new long chain acyl-CoA dehydrogenases", "journal": "Mol. Genet. Metab.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21237683/" } ], "x": 868.6666666666667, "y": 1715.561403508772, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696004", "label": "ACSF2 ligates CoA-SH to MCFA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:48341" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696004", "summary": "ACSF2 ligates CoA-SH to MCFA is a Reactome release 97 curated reaction event. The source record connects MCFA, ATP, CoA-SH to MCFA-CoA, AMP, PPi in mitochondrial matrix and cross-references RHEA:48341.", "equation": "MCFA + ATP + CoA-SH → MCFA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-5690531", "reactome_id": "R-ALL-5690531", "label": "MCFA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59558", "formula": "CO2R", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5690542", "reactome_id": "R-ALL-5690542", "label": "MCFA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90546", "formula": "C22H31N7O17P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of ACSF2 [mitochondrial matrix]", "activity_id": "5696013", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "Q96CM8" ] } ], "citations": [ { "id": "PMID:17762044", "title": "Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome", "journal": "J. Lipid Res.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17762044/" } ], "x": 868.6666666666667, "y": 1667.280701754386, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696007", "label": "ACSF3 ligates CoA-SH to VLCFA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:54537" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696007", "summary": "ACSF3 ligates CoA-SH to VLCFA is a Reactome release 97 curated reaction event. The source record connects VLCFA, ATP, CoA-SH to VLCFA-CoA, AMP, PPi in mitochondrial matrix and cross-references RHEA:54537.", "equation": "VLCFA + ATP + CoA-SH → VLCFA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-5696008", "reactome_id": "R-ALL-5696008", "label": "VLCFA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58950", "formula": "CO2R", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5696003", "reactome_id": "R-ALL-5696003", "label": "VLCFA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:138261", "formula": "C22H31N7O17P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "very long-chain fatty acid-CoA ligase activity of ACSF3 [mitochondrial matrix]", "activity_id": "5696000", "ec_numbers": null, "accessions": [ "Q4G176" ] } ], "citations": [ { "id": "PMID:17762044", "title": "Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome", "journal": "J. Lipid Res.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17762044/" } ], "x": 590, "y": 1540.5438596491229, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696021", "label": "ADPGK:Mg2+ phosphorylates Glc to G6P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:11461" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696021", "summary": "ADPGK:Mg2+ phosphorylates Glc to G6P is a Reactome release 97 curated reaction event. The source record connects Glc, ADP to G6P, AMP, H+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:11461.", "equation": "Glc + ADP → G6P + AMP + H+", "inputs": [ { "node_id": "R-ALL-70113", "reactome_id": "R-ALL-70113", "label": "Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ADP-specific glucokinase activity of ADPGK:Mg2+ [endoplasmic reticulum membrane]", "activity_id": "5696057", "ec_numbers": [ "2.7.1.147" ], "accessions": [] } ], "citations": [ { "id": "PMID:23168256", "title": "T cell activation is driven by an ADP-dependent glucokinase linking enhanced glycolysis with mitochondrial reactive oxygen species generation", "journal": "Cell Rep", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23168256/" }, { "id": "PMID:22219026", "title": "Expression and role in glycolysis of human ADP-dependent glucokinase", "journal": "Mol. Cell. Biochem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22219026/" } ], "x": 868.6666666666667, "y": 481.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696049", "label": "ADPRM hydrolyses ADP-ribose to R5P and AMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10413" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696049", "summary": "ADPRM hydrolyses ADP-ribose to R5P and AMP is a Reactome release 97 curated reaction event. The source record connects ADP-D-ribose, H2O to R5P, AMP, H+ in cytosol and cross-references RHEA:10413.", "equation": "ADP-D-ribose + H2O → R5P + AMP + 2 H+", "inputs": [ { "node_id": "R-ALL-1131972", "reactome_id": "R-ALL-1131972", "label": "ADP-D-ribose", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57967", "formula": "C15H21N5O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ADP-ribose diphosphatase activity of ADPRM:Mn2+ [cytosol]", "activity_id": "5696016", "ec_numbers": [ "3.6.1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:25692488", "title": "Molecular bases of catalysis and ADP-ribose preference of human Mn2+-dependent ADP-ribose/CDP-alcohol diphosphatase and conversion by mutagenesis to a preferential cyclic ADP-ribose phosphohydrolase", "journal": "PLoS ONE", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25692488/" } ], "x": 2660, "y": 3124.3690476190477, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696074", "label": "AGK:Mg2+ phosphorylates MAG, DAG", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial outer membrane · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005741", "GO:0005758" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5696074", "summary": "AGK:Mg2+ phosphorylates MAG, DAG is a Reactome release 97 curated reaction event. The source record connects MAG,DAG, ATP to LPA,PA, ADP in mitochondrial outer membrane · mitochondrial intermembrane space.", "equation": "MAG,DAG + ATP → LPA,PA + ADP", "inputs": [ { "node_id": "R-ALL-5696066", "reactome_id": "R-ALL-5696066", "label": "MAG,DAG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-5696069", "reactome_id": "R-ALL-5696069", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5696037", "reactome_id": "R-ALL-5696037", "label": "LPA,PA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-5696026", "reactome_id": "R-ALL-5696026", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acylglycerol kinase activity of AGK:Mg2+ [mitochondrial outer membrane]", "activity_id": "5696018", "ec_numbers": [ "2.7.1.94" ], "accessions": [] } ], "citations": [ { "id": "PMID:15939762", "title": "A novel acylglycerol kinase that produces lysophosphatidic acid modulates cross talk with EGFR in prostate cancer cells", "journal": "J. Cell Biol.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15939762/" }, { "id": "PMID:25002142", "title": "Proteomic mapping of the human mitochondrial intermembrane space in live cells via ratiometric APEX tagging", "journal": "Mol. Cell", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25002142/" }, { "id": "PMID:1168700", "title": "Congenital cataract and mitochondrial myopathy of skeletal and heart muscle associated with lactic acidosis after exercise", "journal": "J. Pediatr.", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/1168700/" }, { "id": "PMID:22284826", "title": "Lack of the mitochondrial protein acylglycerol kinase causes Sengers syndrome", "journal": "Am. J. Hum. Genet.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22284826/" } ], "x": 2660, "y": 1855.390410958904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696080", "label": "ALDH3B1 oxidises HXAL to PALM", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:33741" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696080", "summary": "ALDH3B1 oxidises HXAL to PALM is a Reactome release 97 curated reaction event. The source record connects HXAL, H2O, NAD+ to PALM, NADH, H+ in plasma membrane · cytosol and cross-references RHEA:33741.", "equation": "HXAL + H2O + NAD+ → PALM + NADH + H+", "inputs": [ { "node_id": "R-ALL-428663", "reactome_id": "R-ALL-428663", "label": "HXAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17600", "formula": "C16H32O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-3632881", "reactome_id": "R-ALL-3632881", "label": "PALM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:7896", "formula": "C16H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldehyde dehydrogenase [NAD(P)+] activity of ALDH3B1 [plasma membrane]", "activity_id": "5696087", "ec_numbers": [ "1.2.1.5" ], "accessions": [ "P43353" ] } ], "citations": [ { "id": "PMID:23721920", "title": "Substrate specificity, plasma membrane localization, and lipid modification of the aldehyde dehydrogenase ALDH3B1", "journal": "Biochim. Biophys. Acta", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23721920/" }, { "id": "PMID:20699116", "title": "Molecular characterization, expression analysis, and role of ALDH3B1 in the cellular protection against oxidative stress", "journal": "Free Radic. Biol. Med.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20699116/" }, { "id": "PMID:17382292", "title": "Expression and initial characterization of human ALDH3B1", "journal": "Biochem Biophys Res Commun", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17382292/" } ], "x": 3321.3333333333335, "y": 1555.4423076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696091", "label": "ALDH1B1 tetramer oxidises CH3CHO to CH3COOH", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:25295" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696091", "summary": "ALDH1B1 tetramer oxidises CH3CHO to CH3COOH is a Reactome release 97 curated reaction event. The source record connects CH3CHO, H2O, NAD+ to CH3COO-, H+, NADH in mitochondrial matrix and cross-references RHEA:25295.", "equation": "CH3CHO + H2O + NAD+ → CH3COO- + H+ + NADH", "inputs": [ { "node_id": "R-ALL-113532", "reactome_id": "R-ALL-113532", "label": "CH3CHO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113539", "reactome_id": "R-ALL-113539", "label": "CH3COO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of ALDH1B1 tetramer [mitochondrial matrix]", "activity_id": "5696088", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:20616185", "title": "Aldehyde dehydrogenase 1B1: molecular cloning and characterization of a novel mitochondrial acetaldehyde-metabolizing enzyme", "journal": "Drug Metab. Dispos.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20616185/" } ], "x": 8018.666666666667, "y": 2844.950980392157, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696119", "label": "AGMO cleaves alkylglycerol into fatty aldehyde and glycerol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5696119", "summary": "AGMO cleaves alkylglycerol into fatty aldehyde and glycerol is a Reactome release 97 curated reaction event. The source record connects alkylglycerol, O2, BH4 to fatty aldehyde, Glycerol, H2O in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "alkylglycerol + O2 + BH4 → fatty aldehyde + Glycerol + H2O + q-BH2", "inputs": [ { "node_id": "R-ALL-5696122", "reactome_id": "R-ALL-5696122", "label": "alkylglycerol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:52575", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5696116", "reactome_id": "R-ALL-5696116", "label": "fatty aldehyde", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35746", "formula": "CHOR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76116", "reactome_id": "R-ALL-76116", "label": "Glycerol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17754", "formula": "C3H8O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1497857", "reactome_id": "R-ALL-1497857", "label": "q-BH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43029", "formula": "C9H13N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glyceryl-ether monooxygenase activity of AGMO [endoplasmic reticulum membrane]", "activity_id": "5696125", "ec_numbers": [ "1.14.16.5" ], "accessions": [ "Q6ZNB7" ] } ], "citations": [ { "id": "PMID:20643956", "title": "Identification of the gene encoding alkylglycerol monooxygenase defines a third class of tetrahydrobiopterin-dependent enzymes", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20643956/" } ], "x": 3600, "y": 2182.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696131", "label": "AOC1 deaminates Hist", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:25626" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696131", "summary": "AOC1 deaminates Hist is a Reactome release 97 curated reaction event. The source record connects Hist, H2O, O2 to I4AH, NH3, H2O2 in extracellular region · plasma membrane and cross-references RHEA:25626.", "equation": "Hist + H2O + O2 → I4AH + NH3 + H2O2 + H+", "inputs": [ { "node_id": "R-ALL-390899", "reactome_id": "R-ALL-390899", "label": "Hist", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58432", "formula": "C5H10N3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-352327", "reactome_id": "R-ALL-352327", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5696128", "reactome_id": "R-ALL-5696128", "label": "I4AH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27398", "formula": "C5H6N2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2022135", "reactome_id": "R-ALL-2022135", "label": "NH3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16134", "formula": "H3N", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351629", "reactome_id": "R-ALL-351629", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "histamine oxidase activity of AOC1 dimer:2xCu2+:4xCa2+ [plasma membrane]", "activity_id": "5696132", "ec_numbers": [ "1.4.3.22" ], "accessions": [] } ], "citations": [ { "id": "PMID:19764817", "title": "Structure and inhibition of human diamine oxidase", "journal": "Biochemistry", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19764817/" } ], "x": 7461.333333333333, "y": 2822.0686274509803, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696146", "label": "AOC2 deaminates TYR", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:30592" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696146", "summary": "AOC2 deaminates TYR is a Reactome release 97 curated reaction event. The source record connects TYR, H2O, O2 to 4HPAA, NH3, H2O2 in extracellular region · plasma membrane and cross-references RHEA:30592.", "equation": "TYR + H2O + O2 → 4HPAA + NH3 + H2O2 + H+", "inputs": [ { "node_id": "R-ALL-5696181", "reactome_id": "R-ALL-5696181", "label": "TYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15760", "formula": "C8H11NO", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-352327", "reactome_id": "R-ALL-352327", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5696178", "reactome_id": "R-ALL-5696178", "label": "4HPAA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15621", "formula": "C8H8O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2022135", "reactome_id": "R-ALL-2022135", "label": "NH3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16134", "formula": "H3N", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351629", "reactome_id": "R-ALL-351629", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "primary methylamine oxidase activity of AOC2 [plasma membrane]", "activity_id": "5696153", "ec_numbers": [ "1.4.3.21" ], "accessions": [ "O75106" ] } ], "citations": [ { "id": "PMID:17400359", "title": "Adipogenesis-related increase of semicarbazide-sensitive amine oxidase and monoamine oxidase in human adipocytes", "journal": "Biochimie", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17400359/" }, { "id": "PMID:19588076", "title": "The unique substrate specificity of human AOC2, a semicarbazide-sensitive amine oxidase", "journal": "Cell. Mol. Life Sci.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19588076/" } ], "x": 7740, "y": 2822.0686274509803, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696183", "label": "AOC3 deaminates BZAM", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:59425" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696183", "summary": "AOC3 deaminates BZAM is a Reactome release 97 curated reaction event. The source record connects BZAM, H2O, O2 to NH3, H2O2, H+ in extracellular region · plasma membrane and cross-references RHEA:59425.", "equation": "BZAM + H2O + O2 → NH3 + H2O2 + H+ + BZAL", "inputs": [ { "node_id": "R-ALL-5696148", "reactome_id": "R-ALL-5696148", "label": "BZAM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:225238", "formula": "C7H10N", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-352327", "reactome_id": "R-ALL-352327", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2022135", "reactome_id": "R-ALL-2022135", "label": "NH3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16134", "formula": "H3N", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351629", "reactome_id": "R-ALL-351629", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5696154", "reactome_id": "R-ALL-5696154", "label": "BZAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17169", "formula": "C7H6O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "primary methylamine oxidase activity of AOC3 dimer:2xCu2+:4xCa2+ [plasma membrane]", "activity_id": "5696159", "ec_numbers": [ "1.4.3.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:9653080", "title": "Cloning of vascular adhesion protein 1 reveals a novel multifunctional adhesion molecule", "journal": "J. Exp. Med.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9653080/" }, { "id": "PMID:17400359", "title": "Adipogenesis-related increase of semicarbazide-sensitive amine oxidase and monoamine oxidase in human adipocytes", "journal": "Biochimie", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17400359/" }, { "id": "PMID:19588076", "title": "The unique substrate specificity of human AOC2, a semicarbazide-sensitive amine oxidase", "journal": "Cell. Mol. Life Sci.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19588076/" } ], "x": 8018.666666666667, "y": 2822.0686274509803, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696213", "label": "AS3MT transfers CH3 from AdoMet to methylarsonite", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11685" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696213", "summary": "AS3MT transfers CH3 from AdoMet to methylarsonite is a Reactome release 97 curated reaction event. The source record connects methylarsonite, AdoMet to dimethylarsinate, AdoHcy in cytosol and cross-references RHEA:11685.", "equation": "methylarsonite + AdoMet → dimethylarsinate + AdoHcy", "inputs": [ { "node_id": "R-ALL-5696224", "reactome_id": "R-ALL-5696224", "label": "methylarsonite", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:14597", "formula": "CH3AsO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5696211", "reactome_id": "R-ALL-5696211", "label": "dimethylarsinate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16223", "formula": "C2H6AsO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arsenite methyltransferase activity of AS3MT [cytosol]", "activity_id": "5696223", "ec_numbers": [ "2.1.1.137" ], "accessions": [ "Q9HBK9" ] } ], "citations": [ { "id": "PMID:16407288", "title": "Human arsenic methyltransferase (AS3MT) pharmacogenetics: gene resequencing and functional genomics studies", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16407288/" } ], "x": 7461.333333333333, "y": 2921.225490196078, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696220", "label": "AS3MT transfers CH3 from AdoMet to arsenite(3-)", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5696220", "summary": "AS3MT transfers CH3 from AdoMet to arsenite(3-) is a Reactome release 97 curated reaction event. The source record connects arsenite(3-), AdoMet to methylarsonate, AdoHcy in cytosol.", "equation": "arsenite(3-) + AdoMet → methylarsonate + AdoHcy", "inputs": [ { "node_id": "R-ALL-5696216", "reactome_id": "R-ALL-5696216", "label": "arsenite(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29242", "formula": "H2AsO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5696214", "reactome_id": "R-ALL-5696214", "label": "methylarsonate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33409", "formula": "CH4AsO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arsenite methyltransferase activity of AS3MT [cytosol]", "activity_id": "5696223", "ec_numbers": [ "2.1.1.137" ], "accessions": [ "Q9HBK9" ] } ], "citations": [ { "id": "PMID:16407288", "title": "Human arsenic methyltransferase (AS3MT) pharmacogenetics: gene resequencing and functional genomics studies", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16407288/" } ], "x": 7461.333333333333, "y": 3104.2843137254904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696230", "label": "GSTO1 dimer reduces methylarsonate to methylarsonite", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15970" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696230", "summary": "GSTO1 dimer reduces methylarsonate to methylarsonite is a Reactome release 97 curated reaction event. The source record connects methylarsonate, GSH to methylarsonite, GSSG, H2O in cytosol and cross-references RHEA:15970.", "equation": "methylarsonate + 2 GSH → methylarsonite + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-5696214", "reactome_id": "R-ALL-5696214", "label": "methylarsonate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33409", "formula": "CH4AsO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5696224", "reactome_id": "R-ALL-5696224", "label": "methylarsonite", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:14597", "formula": "CH3AsO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "methylarsonate reductase activity of GSTO1 dimer [cytosol]", "activity_id": "5696227", "ec_numbers": [ "1.20.4.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11511179", "title": "Human monomethylarsonic acid (MMA(V)) reductase is a member of the glutathione-S-transferase superfamily", "journal": "Chem. Res. Toxicol.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11511179/" } ], "x": 8018.666666666667, "y": 2860.205882352941, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696365", "label": "ASRGL1 hydrolyses aspartame to L-Asp, L-Phe, MeOH", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5696365", "summary": "ASRGL1 hydrolyses aspartame to L-Asp, L-Phe, MeOH is a Reactome release 97 curated reaction event. The source record connects aspartame, H2O to L-Asp, L-Phe, MeOH in cytosol.", "equation": "aspartame + H2O → L-Asp + L-Phe + MeOH", "inputs": [ { "node_id": "R-ALL-5696361", "reactome_id": "R-ALL-5696361", "label": "aspartame", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195544", "formula": "C14H18N2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29448", "reactome_id": "R-ALL-29448", "label": "L-Asp", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29502", "reactome_id": "R-ALL-29502", "label": "L-Phe", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58095", "formula": "C9H11NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359061", "reactome_id": "R-ALL-5359061", "label": "MeOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17790", "formula": "CH4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "omega peptidase activity of ASRGL1 heterodimer [cytosol]", "activity_id": "5696364", "ec_numbers": [ "3.4.19.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:19839645", "title": "The human asparaginase-like protein 1 hASRGL1 is an Ntn hydrolase with beta-aspartyl peptidase activity", "journal": "Biochemistry", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19839645/" }, { "id": "PMID:8141778", "title": "Metabolism of aspartame by human and pig intestinal microvillar peptidases", "journal": "Biochem J", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8141778/" }, { "id": "PMID:22891768", "title": "Uncoupling intramolecular processing and substrate hydrolysis in the N-terminal nucleophile hydrolase hASRGL1 by circular permutation", "journal": "ACS Chem. Biol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22891768/" }, { "id": "PMID:22861376", "title": "Structures of apo and product-bound human L-asparaginase: insights into the mechanism of autoproteolysis and substrate hydrolysis", "journal": "Biochemistry", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22861376/" }, { "id": "PMID:24768817", "title": "Elucidation of the specific function of the conserved threonine triad responsible for human L-asparaginase autocleavage and substrate hydrolysis", "journal": "J. Mol. Biol.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24768817/" } ], "x": 6521.333333333333, "y": 1907.05, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696408", "label": "PXLP-K278-PHYKPL tetramer hydrolyses 5PHL", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:34092" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696408", "summary": "PXLP-K278-PHYKPL tetramer hydrolyses 5PHL is a Reactome release 97 curated reaction event. The source record connects 5PHL, H2O to 2AMAS, Pi, NH4+ in mitochondrial matrix and cross-references RHEA:34092.", "equation": "5PHL + H2O → 2AMAS + Pi + NH4+", "inputs": [ { "node_id": "R-ALL-5696409", "reactome_id": "R-ALL-5696409", "label": "5PHL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57882", "formula": "C6H14N2O6P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113556", "reactome_id": "R-ALL-113556", "label": "2AMAS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58321", "formula": "C6H11NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113561", "reactome_id": "R-ALL-113561", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lyase activity of PHYKPL tetramer [mitochondrial matrix]", "activity_id": "5696417", "ec_numbers": [ "4" ], "accessions": [] } ], "citations": [ { "id": "PMID:22241472", "title": "Molecular identification of hydroxylysine kinase and of ammoniophospholyases acting on 5-phosphohydroxy-L-lysine and phosphoethanolamine", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22241472/" } ], "x": 7078.666666666667, "y": 2049.375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696415", "label": "PXLP-K278-ETNPPL tetramer hydrolyses PETA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:17890" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696415", "summary": "PXLP-K278-ETNPPL tetramer hydrolyses PETA is a Reactome release 97 curated reaction event. The source record connects PETA, H2O to CH3CHO, NH3, Pi in mitochondrial matrix and cross-references RHEA:17890.", "equation": "PETA + H2O → CH3CHO + NH3 + Pi", "inputs": [ { "node_id": "R-ALL-5696414", "reactome_id": "R-ALL-5696414", "label": "PETA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17553", "formula": "C2H8NO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113532", "reactome_id": "R-ALL-113532", "label": "CH3CHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5693978", "reactome_id": "R-ALL-5693978", "label": "NH3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16134", "formula": "H3N", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ethanolamine-phosphate phospho-lyase activity of PXLP-K278-ETNPPL tetramer [mitochondrial matrix]", "activity_id": "5696410", "ec_numbers": [ "4.2.3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:22241472", "title": "Molecular identification of hydroxylysine kinase and of ammoniophospholyases acting on 5-phosphohydroxy-L-lysine and phosphoethanolamine", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22241472/" } ], "x": 2381.3333333333335, "y": 1540.9931506849316, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696424", "label": "AWAT1 transfers acyl group from acyl-CoA to ARACOH, forming wax esters", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:38444" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696424", "summary": "AWAT1 transfers acyl group from acyl-CoA to ARACOH, forming wax esters is a Reactome release 97 curated reaction event. The source record connects ARACOH, Acyl-CoA to arachidyl ester, CoA-SH in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:38444.", "equation": "ARACOH + Acyl-CoA → arachidyl ester + CoA-SH", "inputs": [ { "node_id": "R-ALL-5696426", "reactome_id": "R-ALL-5696426", "label": "ARACOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:75627", "formula": "C20H42O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192172", "reactome_id": "R-ALL-192172", "label": "Acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5696412", "reactome_id": "R-ALL-5696412", "label": "arachidyl ester", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:10036", "formula": "CO2R2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-162743", "reactome_id": "R-ALL-162743", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "long-chain-alcohol O-fatty-acyltransferase activity of AWAT1 [endoplasmic reticulum membrane]", "activity_id": "5696418", "ec_numbers": [ "2.3.1.75" ], "accessions": [ "Q58HT5" ] } ], "citations": [ { "id": "PMID:15671038", "title": "Identification of two novel human acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15671038/" } ], "x": 311.33333333333337, "y": 1682.3684210526317, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696448", "label": "AWAT2 transfers acyl group from acyl-CoA to MAG, forming DAG", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-5696448", "summary": "AWAT2 transfers acyl group from acyl-CoA to MAG, forming DAG is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects MAG, Acyl-CoA to DAG, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "MAG + Acyl-CoA → DAG + CoA-SH", "inputs": [ { "node_id": "R-ALL-5696449", "reactome_id": "R-ALL-5696449", "label": "MAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17408", "formula": "C4H7O4R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-6782195", "reactome_id": "R-ALL-6782195", "label": "Acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-acylglycerol O-acyltransferase activity of AWAT2 [endoplasmic reticulum membrane]", "activity_id": "8848570", "ec_numbers": [ "2.3.1.22" ], "accessions": [ "Q6E213" ] } ], "citations": [ { "id": "PMID:15671038", "title": "Identification of two novel human acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15671038/" }, { "id": "PMID:16106050", "title": "A human skin multifunctional O-acyltransferase that catalyzes the synthesis of acylglycerols, waxes, and retinyl esters", "journal": "J. Lipid Res.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16106050/" } ], "x": 2381.3333333333335, "y": 1818.0890410958905, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-5696457", "label": "BDH2 dehydrogenates 3HBA", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20522" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696457", "summary": "BDH2 dehydrogenates 3HBA is a Reactome release 97 curated reaction event. The source record connects 3HBA, NAD+ to ACA, H+, NADH in cytosol and cross-references RHEA:20522.", "equation": "3HBA + NAD+ → ACA + H+ + NADH", "inputs": [ { "node_id": "R-ALL-5696461", "reactome_id": "R-ALL-5696461", "label": "3HBA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:10983", "formula": "C4H7O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29666", "reactome_id": "R-ALL-29666", "label": "ACA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13705", "formula": "C4H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-hydroxybutyrate dehydrogenase activity of BDH2 tetramer [cytosol]", "activity_id": "5696453", "ec_numbers": [ "1.1.1.30" ], "accessions": [] } ], "citations": [ { "id": "PMID:16380372", "title": "Characterization of human DHRS6, an orphan short chain dehydrogenase/reductase enzyme: a novel, cytosolic type 2 R-beta-hydroxybutyrate dehydrogenase", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16380372/" } ], "x": 8018.666666666667, "y": 311.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696822", "label": "AKR1B15 reduces EST17b to E1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24617" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696822", "summary": "AKR1B15 reduces EST17b to E1 is a Reactome release 97 curated reaction event. The source record connects EST17b, NADP+ to E1, NADPH, H+ in mitochondrial matrix and cross-references RHEA:24617.", "equation": "EST17b + NADP+ → E1 + NADPH + H+", "inputs": [ { "node_id": "R-ALL-5696835", "reactome_id": "R-ALL-5696835", "label": "EST17b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16469", "formula": "C18H24O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5696848", "reactome_id": "R-ALL-5696848", "label": "E1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17263", "formula": "C18H22O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "estradiol 17-beta-dehydrogenase [NAD(P)+] activity of AKR1B15 [mitochondrial matrix]", "activity_id": "5696844", "ec_numbers": [ "1.1.1.62" ], "accessions": [ "C9JRZ8" ] } ], "citations": [ { "id": "PMID:25577493", "title": "Aldo-keto Reductase 1B15 (AKR1B15): a mitochondrial human aldo-keto reductase with activity toward steroids and 3-keto-acyl-CoA conjugates", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25577493/" } ], "x": 4730, "y": 1612.5681818181818, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-5696838", "label": "BHMT2 tetramer transfers CH3 group from SMM to LHCYS", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:26338" ], "source_url": "https://reactome.org/content/detail/R-HSA-5696838", "summary": "BHMT2 tetramer transfers CH3 group from SMM to LHCYS is a Reactome release 97 curated reaction event. The source record connects SMM, HCYS to L-Met, H+ in cytosol and cross-references RHEA:26338.", "equation": "SMM + HCYS → 2 L-Met + H+", "inputs": [ { "node_id": "R-ALL-4789070", "reactome_id": "R-ALL-4789070", "label": "SMM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58252", "formula": "C6H14NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174369", "reactome_id": "R-ALL-174369", "label": "HCYS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58199", "formula": "C4H9NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-174390", "reactome_id": "R-ALL-174390", "label": "L-Met", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57844", "formula": "C5H11NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "S-methylmethionine-homocysteine S-methyltransferase activity of BHMT2:Zn2+ tetramer [cytosol]", "activity_id": "5696821", "ec_numbers": [ "2.1.1.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:18230605", "title": "Betaine-homocysteine S-methyltransferase-2 is an S-methylmethionine-homocysteine methyltransferase", "journal": "J. 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The source record connects THF, HCOOH, ATP to 10-formyl-THF, ADP, Pi in mitochondrial matrix.", "equation": "THF + HCOOH + ATP → 10-formyl-THF + ADP + Pi", "inputs": [ { "node_id": "R-ALL-200671", "reactome_id": "R-ALL-200671", "label": "THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-6801451", "reactome_id": "R-ALL-6801451", "label": "HCOOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5389850", "reactome_id": "R-ALL-5389850", "label": "10-formyl-THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195366", "formula": "C20H21N7O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "formate-tetrahydrofolate ligase activity of MTHFD1L dimer [mitochondrial matrix]", "activity_id": "5696827", "ec_numbers": [ "6.3.4.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:12937168", "title": "Human mitochondrial C1-tetrahydrofolate synthase: gene structure, tissue distribution of the mRNA, and immunolocalization in Chinese hamster ovary calls", "journal": "J. 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Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19948730/" } ], "x": 5008.666666666667, "y": 2962.1363636363635, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6783221", "label": "HAGH hydrolyses (R)-S-LGSH to GSH and LACT", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25246" ], "source_url": "https://reactome.org/content/detail/R-HSA-6783221", "summary": "HAGH hydrolyses (R)-S-LGSH to GSH and LACT is a Reactome release 97 curated reaction event. The source record connects (R)-S-LGSH, H2O to H+, D-LACT, (2Fe-2S)(1+) in cytosol and cross-references RHEA:25246.", "equation": "(R)-S-LGSH + H2O → H+ + D-LACT + (2Fe-2S)(1+)", "inputs": [ { "node_id": "R-ALL-5694130", "reactome_id": "R-ALL-5694130", "label": "(R)-S-LGSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57474", "formula": "C13H20N3O8S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5279872", "reactome_id": "R-ALL-5279872", "label": "D-LACT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16004", "formula": "C3H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2564757", "reactome_id": "R-ALL-2564757", "label": "(2Fe-2S)(1+)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33738", "formula": "Fe2S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydroxyacylglutathione hydrolase activity of HAGH-2:2xZn2+ [cytosol]", "activity_id": "6783215", "ec_numbers": [ "3.1.2.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:8550579", "title": "Molecular cloning, heterologous expression, and characterization of human glyoxalase II", "journal": "J. Biol. Chem.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8550579/" }, { "id": "PMID:15117945", "title": "The Human hydroxyacylglutathione hydrolase (HAGH) gene encodes both cytosolic and mitochondrial forms of glyoxalase II", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15117945/" }, { "id": "PMID:10508780", "title": "Crystal structure of human glyoxalase II and its complex with a glutathione thiolester substrate analogue", "journal": "Structure", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10508780/" } ], "x": 4730, "y": 399.15, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6783880", "label": "PIPOX oxidises PPCA to P6C", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:11993" ], "source_url": "https://reactome.org/content/detail/R-HSA-6783880", "summary": "PIPOX oxidises PPCA to P6C is a Reactome release 97 curated reaction event. The source record connects PPCA, O2 to P6C, H2O2, H+ in peroxisomal matrix and cross-references RHEA:11993.", "equation": "PPCA + O2 → P6C + H2O2 + H+", "inputs": [ { "node_id": "R-ALL-5693325", "reactome_id": "R-ALL-5693325", "label": "PPCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61185", "formula": "C6H11NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6783877", "reactome_id": "R-ALL-6783877", "label": "P6C", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58769", "formula": "C6H8NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-pipecolate oxidase activity of PIPOX [peroxisomal matrix]", "activity_id": "6783871", "ec_numbers": [ "1.5.3.7" ], "accessions": [ "Q9P0Z9" ] } ], "citations": [ { "id": "PMID:10642506", "title": "L-Pipecolic acid oxidase, a human enzyme essential for the degradation of L-pipecolic acid, is most similar to the monomeric sarcosine oxidases", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10642506/" }, { "id": "PMID:24043460", "title": "Lysine metabolism in mammalian brain: an update on the importance of recent discoveries", "journal": "Amino Acids", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24043460/" } ], "x": 6521.333333333333, "y": 2074.2916666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6783883", "label": "P6C transforms to 2AMAS", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix · peroxisomal matrix", "compartment_ids": [ "GO:0005759", "GO:0005782" ], "rhea_ids": [ "RHEA:25123" ], "source_url": "https://reactome.org/content/detail/R-HSA-6783883", "summary": "P6C transforms to 2AMAS is a Reactome release 97 curated reaction event. The source record connects P6C, H2O, H+ to 2AMAS in mitochondrial matrix · peroxisomal matrix and cross-references RHEA:25123.", "equation": "P6C + H2O + H+ → 2AMAS", "inputs": [ { "node_id": "R-ALL-6783877", "reactome_id": "R-ALL-6783877", "label": "P6C", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58769", "formula": "C6H8NO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9960430", "reactome_id": "R-ALL-9960430", "label": "2AMAS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58321", "formula": "C6H11NO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24043460", "title": "Lysine metabolism in mammalian brain: an update on the importance of recent discoveries", "journal": "Amino Acids", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24043460/" } ], "x": 7078.666666666667, "y": 2099.2083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6783939", "label": "PYCR2 decamer reduces (S)-1-pyrroline-5-carboxylate to L-Pro", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14111" ], "source_url": "https://reactome.org/content/detail/R-HSA-6783939", "summary": "PYCR2 decamer reduces (S)-1-pyrroline-5-carboxylate to L-Pro is a Reactome release 97 curated reaction event. The source record connects 1PYR-5COOH, H+, NADPH to L-Pro, NADP+ in cytosol and cross-references RHEA:14111.", "equation": "1PYR-5COOH + 2 H+ + NADPH → L-Pro + NADP+", "inputs": [ { "node_id": "R-ALL-35305", "reactome_id": "R-ALL-35305", "label": "1PYR-5COOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17388", "formula": "C5H6NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113538", "reactome_id": "R-ALL-113538", "label": "L-Pro", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60039", "formula": "C5H9NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyrroline-5-carboxylate reductase activity of PYCR2 decamer [cytosol]", "activity_id": "6783944", "ec_numbers": [ "1.5.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:23024808", "title": "Functional specialization in proline biosynthesis of melanoma", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23024808/" }, { "id": "PMID:9514741", "title": "Crystal structure of human recombinant ornithine aminotransferase.", "journal": "J Mol Biol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9514741/" }, { "id": "PMID:2722838", "title": "Purified human erythrocyte pyrroline-5-carboxylate reductase. Preferential oxidation of NADPH.", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2722838/" }, { "id": "PMID:25865492", "title": "Mutations in PYCR2, Encoding Pyrroline-5-Carboxylate Reductase 2, Cause Microcephaly and Hypomyelination", "journal": "Am. J. Hum. Genet.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25865492/" } ], "x": 7461.333333333333, "y": 1577.85, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6783955", "label": "PYCR3 decamer reduces (S)-1-pyrroline-5-carboxylate to L-Pro", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14111" ], "source_url": "https://reactome.org/content/detail/R-HSA-6783955", "summary": "PYCR3 decamer reduces (S)-1-pyrroline-5-carboxylate to L-Pro is a Reactome release 97 curated reaction event. The source record connects 1PYR-5COOH, H+, NADPH to L-Pro, NADP+ in cytosol and cross-references RHEA:14111.", "equation": "1PYR-5COOH + 2 H+ + NADPH → L-Pro + NADP+", "inputs": [ { "node_id": "R-ALL-35305", "reactome_id": "R-ALL-35305", "label": "1PYR-5COOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17388", "formula": "C5H6NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113538", "reactome_id": "R-ALL-113538", "label": "L-Pro", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60039", "formula": "C5H9NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyrroline-5-carboxylate reductase activity of PYCR3 decamer [cytosol]", "activity_id": "6783951", "ec_numbers": [ "1.5.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:23024808", "title": "Functional specialization in proline biosynthesis of melanoma", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23024808/" }, { "id": "PMID:2722838", "title": "Purified human erythrocyte pyrroline-5-carboxylate reductase. Preferential oxidation of NADPH.", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2722838/" } ], "x": 7740, "y": 1577.85, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6784213", "label": "Unknown hydroxyproline carrier transports cytosolic HPRO into the mitochondrial matrix", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6784213", "summary": "Unknown hydroxyproline carrier transports cytosolic HPRO into the mitochondrial matrix is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects HPRO to HPRO in cytosol · mitochondrial matrix.", "equation": "HPRO → HPRO", "inputs": [ { "node_id": "R-ALL-352049", "reactome_id": "R-ALL-352049", "label": "HPRO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58375", "formula": "C5H9NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6784215", "reactome_id": "R-ALL-6784215", "label": "HPRO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58375", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-hydroxyproline transmembrane transporter activity of HPRO carrier [mitochondrial inner membrane]", "activity_id": "8953315", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8915003", "title": "Carrier-mediated transport controls hydroxyproline catabolism in heart mitochondria from spontaneously hypertensive rat", "journal": "FEBS Lett.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8915003/" } ], "x": 311.33333333333337, "y": 3335.285714285714, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-6784224", "label": "PRODH2:FAD dimer dehydrogenates HPRO to 1-pyrroline-3-hydroxy-5-carboxylate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:52513" ], "source_url": "https://reactome.org/content/detail/R-HSA-6784224", "summary": "PRODH2:FAD dimer dehydrogenates HPRO to 1-pyrroline-3-hydroxy-5-carboxylate is a Reactome release 97 curated reaction event. The source record connects HPRO, quinone to 1PYR-3OH-5COOH, quinol, H+ in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:52513.", "equation": "HPRO + quinone → 1PYR-3OH-5COOH + quinol + H+", "inputs": [ { "node_id": "R-ALL-6784215", "reactome_id": "R-ALL-6784215", "label": "HPRO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58375", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9930628", "reactome_id": "R-ALL-9930628", "label": "quinone", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132124", "formula": "C6O2R4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9929425", "reactome_id": "R-ALL-9929425", "label": "1PYR-3OH-5COOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62612", "formula": "C5H6NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9930630", "reactome_id": "R-ALL-9930630", "label": "quinol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:24646", "formula": "C6H2O2R4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "proline dehydrogenase activity of PRODH2 dimer [mitochondrial inner membrane]", "activity_id": "6784234", "ec_numbers": [ "1.5.5.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:13681368", "title": "Hydroxyproline metabolism. II. Enzymatic preparation and properties of Delta 1-pyrroline-3-hydroxy-5-carboxylic acid", "journal": "J. Biol. Chem.", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/13681368/" }, { "id": "PMID:6250440", "title": "Metabolism of proline and the hydroxyprolines", "journal": "Annu Rev Biochem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6250440/" }, { "id": "PMID:12865426", "title": "Proteomic analysis of the mouse liver mitochondrial inner membrane", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12865426/" }, { "id": "PMID:25697095", "title": "Proline dehydrogenase 2 (PRODH2) is a hydroxyproline dehydrogenase (HYPDH) and molecular target for treating primary hyperoxaluria", "journal": "Biochem. J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25697095/" } ], "x": 868.6666666666667, "y": 3171.476190476191, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6784393", "label": "PXLP-K279-GOT2 dimer transaminates 4-OH-L-glutamate to 4-hydroxy-2-oxoglutarate (HOG)", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31236" ], "source_url": "https://reactome.org/content/detail/R-HSA-6784393", "summary": "PXLP-K279-GOT2 dimer transaminates 4-OH-L-glutamate to 4-hydroxy-2-oxoglutarate (HOG) is a Reactome release 97 curated reaction event. The source record connects 4OH-L-Glu, 2OG to HOG, L-Glu in mitochondrial matrix and cross-references RHEA:31236.", "equation": "4OH-L-Glu + 2OG → HOG + L-Glu", "inputs": [ { "node_id": "R-ALL-9929459", "reactome_id": "R-ALL-9929459", "label": "4OH-L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:6331", "formula": "C5H8NO5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6784229", "reactome_id": "R-ALL-6784229", "label": "HOG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62213", "formula": "C5H4O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(4S)-4-hydroxy-L-glutamate:2-oxoglutarate transaminase activity of GOT2 dimer [mitochondrial matrix]", "activity_id": "6784411", "ec_numbers": [ "2.6.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:13948827", "title": "Metabolism of gamma-hydroxyglutamic acid. I. Conversion to alpha-hydroxy-gamma-ketoglutarate by purified glutamic-aspartic transaminase to rat liver", "journal": "J Biol Chem", "year": 1962, "url": "https://pubmed.ncbi.nlm.nih.gov/13948827/" }, { "id": "PMID:14170323", "title": "Purification of rat-liver gamma-hydroxyglutamate transaminase and its probable identity with glutamate-aspartate transaminase", "journal": "Biochim. Biophys. Acta", "year": 1964, "url": "https://pubmed.ncbi.nlm.nih.gov/14170323/" } ], "x": 868.6666666666667, "y": 3269.761904761905, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6784402", "label": "ALDH4A1 converts 1PYR-3OH-5COOH to 4-OH-L-glutamate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31156" ], "source_url": "https://reactome.org/content/detail/R-HSA-6784402", "summary": "ALDH4A1 converts 1PYR-3OH-5COOH to 4-OH-L-glutamate is a Reactome release 97 curated reaction event. The source record connects H2O, 1PYR-3OH-5COOH, NAD+ to 4OH-L-Glu, H+, NADH in mitochondrial matrix and cross-references RHEA:31156.", "equation": "2 H2O + 1PYR-3OH-5COOH + NAD+ → 4OH-L-Glu + H+ + NADH", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9929425", "reactome_id": "R-ALL-9929425", "label": "1PYR-3OH-5COOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62612", "formula": "C5H6NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9929459", "reactome_id": "R-ALL-9929459", "label": "4OH-L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:6331", "formula": "C5H8NO5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-glutamate gamma-semialdehyde dehydrogenase activity of ALDH4A1 dimer [mitochondrial matrix]", "activity_id": "70678", "ec_numbers": [ "1.2.1.88" ], "accessions": [] } ], "citations": [ { "id": "PMID:13681370", "title": "Hydroxyproline metabolism. IV. Enzymatic synthesis of gamma-hydroxyglutamate from Delta 1-pyrroline-3-hydroxy-5-carboxylate", "journal": "J Biol Chem", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/13681370/" }, { "id": "PMID:22516612", "title": "The three-dimensional structural basis of type II hyperprolinemia", "journal": "J. Mol. Biol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22516612/" }, { "id": "PMID:500817", "title": "Genetic evidence for a common enzyme catalyzing the second step in the degradation of proline and hydroxyproline", "journal": "J Clin Invest", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/500817/" } ], "x": 590, "y": 3138.714285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6784423", "label": "HOGA1 tetramer aldol-cleaves 4-OH-2-oxoglutarate (HOG) to glyoxylate and pyruvate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18170", "RHEA:30688" ], "source_url": "https://reactome.org/content/detail/R-HSA-6784423", "summary": "HOGA1 tetramer aldol-cleaves 4-OH-2-oxoglutarate (HOG) to glyoxylate and pyruvate is a Reactome release 97 curated reaction event. The source record connects HOG to glyoxylate, PYR in mitochondrial matrix and cross-references RHEA:18170, RHEA:30688.", "equation": "HOG → glyoxylate + PYR", "inputs": [ { "node_id": "R-ALL-6784229", "reactome_id": "R-ALL-6784229", "label": "HOG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62213", "formula": "C5H4O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-904849", "reactome_id": "R-ALL-904849", "label": "glyoxylate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(R,S)-4-hydroxy-2-oxoglutarate aldolase activity of HOGA1 tetramer [mitochondrial matrix]", "activity_id": "6784396", "ec_numbers": [ "4.1.3.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:21998747", "title": "Structural and biochemical studies of human 4-hydroxy-2-oxoglutarate aldolase: implications for hydroxyproline metabolism in primary hyperoxaluria", "journal": "PLoS ONE", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21998747/" } ], "x": 590, "y": 3237, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6784434", "label": "An unknown carrier transports cytosolic glyoxylate to the peroxisome", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "cytosol · peroxisomal matrix", "compartment_ids": [ "GO:0005829", "GO:0005782" ], "rhea_ids": [ "RHEA:29723" ], "source_url": "https://reactome.org/content/detail/R-HSA-6784434", "summary": "An unknown carrier transports cytosolic glyoxylate to the peroxisome is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects glyoxylate to glyoxylate in cytosol · peroxisomal matrix and cross-references RHEA:29723.", "equation": "glyoxylate → glyoxylate", "inputs": [ { "node_id": "R-ALL-5367039", "reactome_id": "R-ALL-5367039", "label": "glyoxylate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389678", "reactome_id": "R-ALL-389678", "label": "glyoxylate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transmembrane transporter activity of peroxisomal glyoxylate carrier [peroxisomal membrane]", "activity_id": "8953408", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:26858947", "title": "Metabolic Interplay between Peroxisomes and Other Subcellular Organelles Including Mitochondria and the Endoplasmic Reticulum", "journal": "Front Cell Dev Biol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26858947/" } ], "x": 311.33333333333337, "y": 3269.761904761905, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-6784436", "label": "An unknown carrier transports mitochondrial glyoxylate to the cytosol", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [ "RHEA:29723" ], "source_url": "https://reactome.org/content/detail/R-HSA-6784436", "summary": "An unknown carrier transports mitochondrial glyoxylate to the cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects glyoxylate to glyoxylate in cytosol · mitochondrial matrix and cross-references RHEA:29723.", "equation": "glyoxylate → glyoxylate", "inputs": [ { "node_id": "R-ALL-904849", "reactome_id": "R-ALL-904849", "label": "glyoxylate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5367039", "reactome_id": "R-ALL-5367039", "label": "glyoxylate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transmembrane transporter activity of plasma membrane glyoxylate carrier [plasma membrane]", "activity_id": "8953411", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:26858947", "title": "Metabolic Interplay between Peroxisomes and Other Subcellular Organelles Including Mitochondria and the Endoplasmic Reticulum", "journal": "Front Cell Dev Biol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26858947/" } ], "x": 590, "y": 3269.761904761905, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-6784959", "label": "BPHL hydrolyses VACV to ACV", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:83872" ], "source_url": "https://reactome.org/content/detail/R-HSA-6784959", "summary": "BPHL hydrolyses VACV to ACV is a Reactome release 97 curated reaction event. The source record connects VACV, H2O to ACV, L-Val in cytosol · mitochondrial outer membrane and cross-references RHEA:83872.", "equation": "VACV + H2O → ACV + L-Val", "inputs": [ { "node_id": "R-ALL-6784964", "reactome_id": "R-ALL-6784964", "label": "VACV", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35854", "formula": "C13H20N6O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-561052", "reactome_id": "R-ALL-561052", "label": "ACV", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:2453", "formula": "C8H11N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29702", "reactome_id": "R-ALL-29702", "label": "L-Val", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57762", "formula": "C5H11NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alpha-amino-acid esterase activity of BPHL [mitochondrial outer membrane]", "activity_id": "6784966", "ec_numbers": [ "3.1.1.43" ], "accessions": [ "Q86WA6" ] } ], "citations": [ { "id": "PMID:12732646", "title": "Identification of a human valacyclovirase: biphenyl hydrolase-like protein as valacyclovir hydrolase", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12732646/" }, { "id": "PMID:18256025", "title": "Molecular basis of prodrug activation by human valacyclovirase, an alpha-amino acid ester hydrolase", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18256025/" } ], "x": 7740, "y": 2921.225490196078, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6785928", "label": "CHAC1,2 cleaves GSH to OPRO and CysGly", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:47725" ], "source_url": "https://reactome.org/content/detail/R-HSA-6785928", "summary": "CHAC1,2 cleaves GSH to OPRO and CysGly is a Reactome release 97 curated reaction event. The source record connects GSH to OPRO, CysGly in cytosol and cross-references RHEA:47725.", "equation": "GSH → OPRO + CysGly", "inputs": [ { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1247942", "reactome_id": "R-ALL-1247942", "label": "OPRO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58402", "formula": "C5H6NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1247904", "reactome_id": "R-ALL-1247904", "label": "CysGly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61694", "formula": "C5H10N2O3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione specific gamma-glutamylcyclotransferase activity of CHAC1,2 [cytosol]", "activity_id": "6785934", "ec_numbers": [ "4.3.2.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:19109178", "title": "CHAC1/MGC4504 is a novel proapoptotic component of the unfolded protein response, downstream of the ATF4-ATF3-CHOP cascade", "journal": "J. Immunol.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19109178/" }, { "id": "PMID:25931127", "title": "Human CHAC1 Protein Degrades Glutathione, and mRNA Induction Is Regulated by the Transcription Factors ATF4 and ATF3 and a Bipartite ATF/CRE Regulatory Element", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25931127/" } ], "x": 868.6666666666667, "y": 3556.333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6785933", "label": "UGT8 transfers Gal from UDP-Gal to CERA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:48344" ], "source_url": "https://reactome.org/content/detail/R-HSA-6785933", "summary": "UGT8 transfers Gal from UDP-Gal to CERA is a Reactome release 97 curated reaction event. The source record connects CERA, UDP-Gal to GalCer, UDP, H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:48344.", "equation": "CERA + UDP-Gal → GalCer + UDP + H+", "inputs": [ { "node_id": "R-ALL-428256", "reactome_id": "R-ALL-428256", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1981137", "reactome_id": "R-ALL-1981137", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6785915", "reactome_id": "R-ALL-6785915", "label": "GalCer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36498", "formula": "C10H17NO8R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158602", "reactome_id": "R-ALL-158602", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N-acylsphingosine galactosyltransferase activity of UGT8 [endoplasmic reticulum membrane]", "activity_id": "6785918", "ec_numbers": [ "2.4.1.47" ], "accessions": [ "Q16880" ] } ], "citations": [ { "id": "PMID:9125199", "title": "Cloning, characterization, and expression of human ceramide galactosyltransferase cDNA", "journal": "Biochem. Biophys. Res. Commun.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9125199/" }, { "id": "PMID:23290777", "title": "Ceramide glycosylation catalyzed by glucosylceramide synthase and cancer drug resistance", "journal": "Adv. Cancer Res.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23290777/" }, { "id": "PMID:9748263", "title": "UDP-galactose:ceramide galactosyltransferase is a class I integral membrane protein of the endoplasmic reticulum", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9748263/" } ], "x": 3600, "y": 1570.4038461538462, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6786190", "label": "CMBL hydrolyses OM to OLMS", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6786190", "summary": "CMBL hydrolyses OM to OLMS is a Reactome release 97 curated reaction event. The source record connects OM, H2O to diketone, OLMS in cytosol.", "equation": "OM + H2O → diketone + OLMS", "inputs": [ { "node_id": "R-ALL-6786175", "reactome_id": "R-ALL-6786175", "label": "OM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:31932", "formula": "C29H30N6O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6786179", "reactome_id": "R-ALL-6786179", "label": "diketone", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:46640", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9838649", "reactome_id": "R-ALL-9838649", "label": "OLMS", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false } ], "catalysts": [ { "label": "hydrolase activity of CMBL [cytosol]", "activity_id": "6786202", "ec_numbers": [ "3" ], "accessions": [ "Q96DG6" ] } ], "citations": [ { "id": "PMID:20177059", "title": "Human carboxymethylenebutenolidase as a bioactivating hydrolase of olmesartan medoxomil in liver and intestine", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20177059/" } ], "x": 7461.333333333333, "y": 3119.5392156862745, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6786239", "label": "CYP4V2 omega-hydroxylates DHA to HDoHE", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6786239", "summary": "CYP4V2 omega-hydroxylates DHA to HDoHE is a Reactome release 97 curated reaction event. The source record connects DHA, H+, NADPH to HDoHE, H2O, NADP+ in endoplasmic reticulum membrane · cytosol.", "equation": "DHA + H+ + NADPH + O2 → HDoHE + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-6786220", "reactome_id": "R-ALL-6786220", "label": "DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6786225", "reactome_id": "R-ALL-6786225", "label": "HDoHE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77015", "formula": "C22H31O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen of CYP4V2 [e…", "activity_id": "6786235", "ec_numbers": [ "1.14.13" ], "accessions": [ "Q6ZWL3" ] } ], "citations": [ { "id": "PMID:15042513", "title": "Bietti crystalline corneoretinal dystrophy is caused by mutations in the novel gene CYP4V2", "journal": "Am J Hum Genet", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15042513/" }, { "id": "PMID:19661213", "title": "Expression and characterization of CYP4V2 as a fatty acid omega-hydroxylase", "journal": "Drug Metab. Dispos.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19661213/" }, { "id": "PMID:22772592", "title": "CYP4V2 in Bietti's crystalline dystrophy: ocular localization, metabolism of ω-3-polyunsaturated fatty acids, and functional deficit of the p.H331P variant", "journal": "Mol. Pharmacol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22772592/" } ], "x": 7461.333333333333, "y": 3028.0098039215686, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6786245", "label": "CARNS1 transforms ATP, L-His, b-Ala to CARN", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19298" ], "source_url": "https://reactome.org/content/detail/R-HSA-6786245", "summary": "CARNS1 transforms ATP, L-His, b-Ala to CARN is a Reactome release 97 curated reaction event. The source record connects ATP, L-His, b-Ala to CARN, ADP, Pi in cytosol and cross-references RHEA:19298.", "equation": "ATP + L-His + b-Ala → CARN + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29612", "reactome_id": "R-ALL-29612", "label": "L-His", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57595", "formula": "C6H9N3O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73590", "reactome_id": "R-ALL-73590", "label": "b-Ala", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57966", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6786250", "reactome_id": "R-ALL-6786250", "label": "CARN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57485", "formula": "C9H14N4O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carnosine synthase activity of CARNS1 [cytosol]", "activity_id": "6786246", "ec_numbers": [ "6.3.2.11" ], "accessions": [ "A5YM72" ] } ], "citations": [ { "id": "PMID:20097752", "title": "Molecular identification of carnosine synthase as ATP-grasp domain-containing protein 1 (ATPGD1)", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20097752/" } ], "x": 7461.333333333333, "y": 2030.6875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6786257", "label": "DCTPP1 hydrolyses 5idCTP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6786257", "summary": "DCTPP1 hydrolyses 5idCTP is a Reactome release 97 curated reaction event. The source record connects 5idCTP, H2O to 5idCMP, PPi, H+ in cytosol.", "equation": "5idCTP + H2O → 5idCMP + PPi + H+", "inputs": [ { "node_id": "R-ALL-6786348", "reactome_id": "R-ALL-6786348", "label": "5idCTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:86351", "formula": "C9H15IN3O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6786373", "reactome_id": "R-ALL-6786373", "label": "5idCMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43263", "formula": "C9H13IN3O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dCTP diphosphatase activity of DCTPP1 tetramer [cytosol]", "activity_id": "6786252", "ec_numbers": [ "3.6.1.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:19220460", "title": "Mouse RS21-C6 is a mammalian 2'-deoxycytidine 5'-triphosphate pyrophosphohydrolase that prefers 5-iodocytosine", "journal": "FEBS J.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19220460/" }, { "id": "PMID:17320107", "title": "Crystal structure of RS21-C6, involved in nucleoside triphosphate pyrophosphohydrolysis", "journal": "J. Mol. Biol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17320107/" } ], "x": 3600, "y": 3173.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6786650", "label": "DDHD1,2 hydrolyse PA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6786650", "summary": "DDHD1,2 hydrolyse PA is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PA, H2O to 1-acyl LPA, LCFA(-) in cytosol · plasma membrane.", "equation": "PA + H2O → 1-acyl LPA + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1602448", "reactome_id": "R-ALL-1602448", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1602447", "reactome_id": "R-ALL-1602447", "label": "1-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipase activity of DDHD1,2 [cytosol]", "activity_id": "6786648", "ec_numbers": [ "3.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:22922100", "title": "Roles of SAM and DDHD domains in mammalian intracellular phospholipase A1 KIAA0725p", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22922100/" }, { "id": "PMID:23176821", "title": "Alteration of fatty-acid-metabolizing enzymes affects mitochondrial form and function in hereditary spastic paraplegia", "journal": "Am. J. Hum. Genet.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23176821/" }, { "id": "PMID:11788596", "title": "A novel phospholipase A1 with sequence homology to a mammalian Sec23p-interacting protein, p125", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11788596/" }, { "id": "PMID:23176823", "title": "Mutations in DDHD2, encoding an intracellular phospholipase A(1), cause a recessive form of complex hereditary spastic paraplegia", "journal": "Am. J. Hum. Genet.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23176823/" } ], "x": 2660, "y": 1818.0890410958905, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-6786720", "label": "DECR2 reduces a (2E,4E)-dienoyl-CoA to a 4,5-saturated-(3E)-enoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix · peroxisomal membrane", "compartment_ids": [ "GO:0005782", "GO:0005778" ], "rhea_ids": [ "RHEA:45913" ], "source_url": "https://reactome.org/content/detail/R-HSA-6786720", "summary": "DECR2 reduces a (2E,4E)-dienoyl-CoA to a 4,5-saturated-(3E)-enoyl-CoA is a Reactome release 97 curated reaction event. The source record connects NADPH, H+, (2E,4E)-dienoyl-CoA to NADP+, 4,5-saturated-(3E)-enoyl-CoA(4-) in peroxisomal matrix · peroxisomal membrane and cross-references RHEA:45913.", "equation": "NADPH + H+ + (2E,4E)-dienoyl-CoA → NADP+ + 4,5-saturated-(3E)-enoyl-CoA(4-)", "inputs": [ { "node_id": "R-ALL-113602", "reactome_id": "R-ALL-113602", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9914117", "reactome_id": "R-ALL-9914117", "label": "(2E,4E)-dienoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:85101", "formula": "C26H35N7O17P3SR", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389556", "reactome_id": "R-ALL-389556", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9914123", "reactome_id": "R-ALL-9914123", "label": "4,5-saturated-(3E)-enoyl-CoA(4-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:85493", "formula": "C26H37N7O17P3SR", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "trans-2-enoyl-CoA reductase (NADPH) activity of DECR2 [peroxisomal membrane]", "activity_id": "6786714", "ec_numbers": [ "1.3.1.38" ], "accessions": [ "Q9NUI1" ] } ], "citations": [ { "id": "PMID:11514237", "title": "Characterisation of human peroxisomal 2,4-dienoyl-CoA reductase", "journal": "Biochim. Biophys. Acta", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11514237/" }, { "id": "PMID:22745130", "title": "Studies of human 2,4-dienoyl CoA reductase shed new light on peroxisomal β-oxidation of unsaturated fatty acids", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22745130/" } ], "x": 590, "y": 1673.3157894736842, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6787321", "label": "DERA cleaves dR5P to GA3P and CH3CHO", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12822" ], "source_url": "https://reactome.org/content/detail/R-HSA-6787321", "summary": "DERA cleaves dR5P to GA3P and CH3CHO is a Reactome release 97 curated reaction event. The source record connects dR5P to GA3P, CH3CHO in cytosol and cross-references RHEA:12822.", "equation": "dR5P → GA3P + CH3CHO", "inputs": [ { "node_id": "R-ALL-6787328", "reactome_id": "R-ALL-6787328", "label": "dR5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62877", "formula": "C5H9O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29510", "reactome_id": "R-ALL-29510", "label": "CH3CHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "deoxyribose-phosphate aldolase activity of DERA [cytosol]", "activity_id": "6787320", "ec_numbers": [ "4.1.2.4" ], "accessions": [ "Q9Y315" ] } ], "citations": [ { "id": "PMID:25229427", "title": "DERA is the human deoxyribose phosphate aldolase and is involved in stress response", "journal": "Biochim. Biophys. Acta", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25229427/" } ], "x": 311.33333333333337, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6787329", "label": "PGM2:Mg2+ isomerises R1P to R5P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18794" ], "source_url": "https://reactome.org/content/detail/R-HSA-6787329", "summary": "PGM2:Mg2+ isomerises R1P to R5P is a Reactome release 97 curated reaction event. The source record connects R1P to R5P in cytosol and cross-references RHEA:18794.", "equation": "R1P → R5P", "inputs": [ { "node_id": "R-ALL-112016", "reactome_id": "R-ALL-112016", "label": "R1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57720", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphopentomutase activity of PGM2:Mg2+ [cytosol]", "activity_id": "6787332", "ec_numbers": [ "5.4.2.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:17804405", "title": "Molecular identification of mammalian phosphopentomutase and glucose-1,6-bisphosphate synthase, two members of the alpha-D-phosphohexomutase family", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17804405/" } ], "x": 868.6666666666667, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6787811", "label": "HAO2 tetramer oxidises 2OH-PALM", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:67945" ], "source_url": "https://reactome.org/content/detail/R-HSA-6787811", "summary": "HAO2 tetramer oxidises 2OH-PALM is a Reactome release 97 curated reaction event. The source record connects 2OH-PALM, O2 to 2oxo-PALM, H2O2 in peroxisomal matrix and cross-references RHEA:67945.", "equation": "2OH-PALM + O2 → 2oxo-PALM + H2O2", "inputs": [ { "node_id": "R-ALL-6787810", "reactome_id": "R-ALL-6787810", "label": "2OH-PALM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:65097", "formula": "C16H31O3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6787814", "reactome_id": "R-ALL-6787814", "label": "2oxo-PALM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:176593", "formula": "C16H29O3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(S)-2-hydroxy-acid oxidase activity of HAO2 tetramer [peroxisomal matrix]", "activity_id": "6787809", "ec_numbers": [ "1.1.3.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:10777549", "title": "Identification and characterization of HAOX1, HAOX2, and HAOX3, three human peroxisomal 2-hydroxy acid oxidases", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10777549/" } ], "x": 311.33333333333337, "y": 1588.8245614035088, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6788295", "label": "HDHD1:Mg2+ dephosphorylates PURIDP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10945" ], "source_url": "https://reactome.org/content/detail/R-HSA-6788295", "summary": "HDHD1:Mg2+ dephosphorylates PURIDP is a Reactome release 97 curated reaction event. The source record connects PURIDP, H2O to PURID, Pi in cytosol and cross-references RHEA:10945.", "equation": "PURIDP + H2O → PURID + Pi", "inputs": [ { "node_id": "R-ALL-6788291", "reactome_id": "R-ALL-6788291", "label": "PURIDP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58380", "formula": "C9H11N2O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6788290", "reactome_id": "R-ALL-6788290", "label": "PURID", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17802", "formula": "C9H12N2O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity of HDHD1:Mg2+ [cytosol]", "activity_id": "6788289", "ec_numbers": [ "3" ], "accessions": [] } ], "citations": [ { "id": "PMID:20722631", "title": "HDHD1, which is often deleted in X-linked ichthyosis, encodes a pseudouridine-5'-phosphatase", "journal": "Biochem. J.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20722631/" } ], "x": 3878.666666666667, "y": 2856.3928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6788523", "label": "NUBPL transfers 4Fe-4S to Complex I subunits", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6788523", "summary": "NUBPL transfers 4Fe-4S to Complex I subunits is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects NDUF subunits, NUBPL:4Fe-4S to NDUF:4Fe-4S subunits, NUBPL in mitochondrial matrix.", "equation": "NDUF subunits + NUBPL:4Fe-4S → NDUF:4Fe-4S subunits + NUBPL", "inputs": [ { "node_id": "R-HSA-6788518", "reactome_id": "R-HSA-6788518", "label": "NDUF subunits", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-5690007", "reactome_id": "R-HSA-5690007", "label": "NUBPL:4Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6788527", "reactome_id": "R-HSA-6788527", "label": "NDUF:4Fe-4S subunits", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-5689133", "reactome_id": "R-HSA-5689133", "label": "NUBPL", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" } ], "x": 4730, "y": 1092.1, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-6788556", "label": "NUBPL transfers 4Fe-4S to NDUFV1, V2", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6788556", "summary": "NUBPL transfers 4Fe-4S to NDUFV1, V2 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects NDUFV1, NUBPL:4Fe-4S, NDUFV2 to NDUFV1:4Fe-4S:FMN, NDUFV2:4Fe-4S, NUBPL in mitochondrial matrix · mitochondrial inner membrane.", "equation": "NDUFV1 + 2 NUBPL:4Fe-4S + NDUFV2 + FMN → NDUFV1:4Fe-4S:FMN + NDUFV2:4Fe-4S + 2 NUBPL", "inputs": [ { "node_id": "R-HSA-163926", "reactome_id": "R-HSA-163926", "label": "NDUFV1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5690007", "reactome_id": "R-HSA-5690007", "label": "NUBPL:4Fe-4S", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-163939", "reactome_id": "R-HSA-163939", "label": "NDUFV2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9864491", "reactome_id": "R-ALL-9864491", "label": "FMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58210", "formula": "C17H18N4O9P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6788516", "reactome_id": "R-HSA-6788516", "label": "NDUFV1:4Fe-4S:FMN", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-6788524", "reactome_id": "R-HSA-6788524", "label": "NDUFV2:4Fe-4S", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-5689133", "reactome_id": "R-HSA-5689133", "label": "NUBPL", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" }, { "id": "PMID:9571201", "title": "Cloning of the human mitochondrial 51 kDa subunit (NDUFV1) reveals a 100% antisense homology of its 3'UTR with the 5'UTR of the gamma-interferon inducible protein (IP-30) precursor: is this a link between mitochondrial myopathy and inflammation?", "journal": "Biochem. Biophys. Res. Commun.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9571201/" } ], "x": 4730, "y": 1066.1666666666667, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-6788597", "label": "HMGCLL1:Mg2+ cleaves bHMG-CoA to Ac-CoA and ACA", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24405" ], "source_url": "https://reactome.org/content/detail/R-HSA-6788597", "summary": "HMGCLL1:Mg2+ cleaves bHMG-CoA to Ac-CoA and ACA is a Reactome release 97 curated reaction event. The source record connects bHMG-CoA to Ac-CoA, ACA in cytosol and cross-references RHEA:24405.", "equation": "bHMG-CoA → Ac-CoA + ACA", "inputs": [ { "node_id": "R-ALL-191374", "reactome_id": "R-ALL-191374", "label": "bHMG-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43074", "formula": "C27H39N7O20P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29666", "reactome_id": "R-ALL-29666", "label": "ACA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13705", "formula": "C4H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydroxymethylglutaryl-CoA lyase activity of HMGCLL1:Mg2+ [cytosol]", "activity_id": "6788588", "ec_numbers": [ "4.1.3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:22865860", "title": "Identification and characterization of an extramitochondrial human 3-hydroxy-3-methylglutaryl-CoA lyase", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22865860/" }, { "id": "PMID:22847177", "title": "Characterization of a novel HMG-CoA lyase enzyme with a dual location in endoplasmic reticulum and cytosol", "journal": "J. Lipid Res.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22847177/" } ], "x": 7740, "y": 506.125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6788611", "label": "HYKK phosphorylates 5HLYS", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:19050" ], "source_url": "https://reactome.org/content/detail/R-HSA-6788611", "summary": "HYKK phosphorylates 5HLYS is a Reactome release 97 curated reaction event. The source record connects 5HLYS, GTP to 5PHL, GDP, H+ in mitochondrial matrix and cross-references RHEA:19050.", "equation": "5HLYS + GTP → 5PHL + GDP + H+", "inputs": [ { "node_id": "R-ALL-6788606", "reactome_id": "R-ALL-6788606", "label": "5HLYS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58357", "formula": "C6H15N2O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113573", "reactome_id": "R-ALL-113573", "label": "GTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37565", "formula": "C10H12N5O14P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5696409", "reactome_id": "R-ALL-5696409", "label": "5PHL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57882", "formula": "C6H14N2O6P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113525", "reactome_id": "R-ALL-113525", "label": "GDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58189", "formula": "C10H12N5O11P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "hydroxylysine kinase activity of HYKK [mitochondrial matrix]", "activity_id": "6788609", "ec_numbers": [ "2.7.1.81" ], "accessions": [ "A2RU49" ] } ], "citations": [ { "id": "PMID:22241472", "title": "Molecular identification of hydroxylysine kinase and of ammoniophospholyases acting on 5-phosphohydroxy-L-lysine and phosphoethanolamine", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22241472/" } ], "x": 6800, "y": 2074.2916666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6788798", "label": "AK4 phosphorylates (d)NMPs to (d)NDPs", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12974", "RHEA:23101", "RHEA:11601", "RHEA:25095" ], "source_url": "https://reactome.org/content/detail/R-HSA-6788798", "summary": "AK4 phosphorylates (d)NMPs to (d)NDPs is a Reactome release 97 curated reaction event. The source record connects (d)NMPs, ATP to (d)NDPs, ADP in mitochondrial matrix and cross-references RHEA:12974, RHEA:23101, RHEA:11601, RHEA:25095.", "equation": "(d)NMPs + ATP → (d)NDPs + ADP", "inputs": [ { "node_id": "R-ALL-6788802", "reactome_id": "R-ALL-6788802", "label": "(d)NMPs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6788801", "reactome_id": "R-ALL-6788801", "label": "(d)NDPs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside monophosphate kinase activity of AK4 [mitochondrial matrix]", "activity_id": "6788795", "ec_numbers": [ "2.7.4.4" ], "accessions": [ "P27144" ] } ], "citations": [ { "id": "PMID:19766732", "title": "Evidence of an intact N-terminal translocation sequence of human mitochondrial adenylate kinase 4", "journal": "Int. J. Biochem. Cell Biol.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19766732/" } ], "x": 3878.666666666667, "y": 3061.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6788810", "label": "AK6 phosphorylates (d)NMPs to (d)NDPs", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:23101", "RHEA:12974", "RHEA:11601", "RHEA:25095" ], "source_url": "https://reactome.org/content/detail/R-HSA-6788810", "summary": "AK6 phosphorylates (d)NMPs to (d)NDPs is a Reactome release 97 curated reaction event. The source record connects (d)NMPs, ATP to (d)NDPs, ADP in nucleoplasm and cross-references RHEA:23101, RHEA:12974, RHEA:11601, RHEA:25095.", "equation": "(d)NMPs + ATP → (d)NDPs + ADP", "inputs": [ { "node_id": "R-ALL-6788813", "reactome_id": "R-ALL-6788813", "label": "(d)NMPs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6788807", "reactome_id": "R-ALL-6788807", "label": "(d)NDPs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside monophosphate kinase activity of AK6 [nucleoplasm]", "activity_id": "6788811", "ec_numbers": [ "2.7.4.4" ], "accessions": [ "Q9Y3D8" ] } ], "citations": [ { "id": "PMID:15630091", "title": "The crystal structure of human adenylate kinase 6: An adenylate kinase localized to the cell nucleus", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15630091/" } ], "x": 3878.666666666667, "y": 3073.6458333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6792445", "label": "LIPH, I hydrolyse PA to 2-acyl LPA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6792445", "summary": "LIPH, I hydrolyse PA to 2-acyl LPA is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PA, H2O to 2-acyl LPA, LCFA(-) in plasma membrane · extracellular region.", "equation": "PA + H2O → 2-acyl LPA + LCFA(-)", "inputs": [ { "node_id": "R-ALL-1602448", "reactome_id": "R-ALL-1602448", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6792472", "reactome_id": "R-ALL-6792472", "label": "2-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17936", "formula": "C4H8O7PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipase activity of LIPH, I [plasma membrane]", "activity_id": "6792469", "ec_numbers": [ "3.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12063250", "title": "A novel phosphatidic acid-selective phospholipase A1 that produces lysophosphatidic acid", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12063250/" }, { "id": "PMID:12963729", "title": "Biochemical and molecular characterization of two phosphatidic acid-selective phospholipase A1s, mPA-PLA1alpha and mPA-PLA1beta", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12963729/" } ], "x": 2938.666666666667, "y": 1818.0890410958905, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-6798317", "label": "GNMT tetramer transfers methyl group from AdoMet to Gly to form AdoHyc and SARC", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19938" ], "source_url": "https://reactome.org/content/detail/R-HSA-6798317", "summary": "GNMT tetramer transfers methyl group from AdoMet to Gly to form AdoHyc and SARC is a Reactome release 97 curated reaction event. The source record connects AdoMet, Gly to AdoHcy, SARC, H+ in cytosol and cross-references RHEA:19938.", "equation": "AdoMet + Gly → AdoHcy + SARC + H+", "inputs": [ { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359017", "reactome_id": "R-ALL-5359017", "label": "SARC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57433", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycine N-methyltransferase activity of GNMT tetramer [cytosol]", "activity_id": "6798320", "ec_numbers": [ "2.1.1.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:15340920", "title": "Glycine N-methyltransferases: a comparison of the crystal structures and kinetic properties of recombinant human, mouse and rat enzymes", "journal": "Proteins", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15340920/" } ], "x": 311.33333333333337, "y": 3204.2380952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6798335", "label": "BPGM dimer isomerises 1,3BPG to 2,3BPG", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17766" ], "source_url": "https://reactome.org/content/detail/R-HSA-6798335", "summary": "BPGM dimer isomerises 1,3BPG to 2,3BPG is a Reactome release 97 curated reaction event. The source record connects 1,3BPG to 2,3BPG, H+ in cytosol and cross-references RHEA:17766.", "equation": "1,3BPG → 2,3BPG + H+", "inputs": [ { "node_id": "R-ALL-29800", "reactome_id": "R-ALL-29800", "label": "1,3BPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57604", "formula": "C3H4O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6798337", "reactome_id": "R-ALL-6798337", "label": "2,3BPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58248", "formula": "C3H3O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "bisphosphoglycerate mutase activity of BPGM dimer [cytosol]", "activity_id": "6798336", "ec_numbers": [ "5.4.2.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:5687724", "title": "The purification and properties of diphosphoglycerate mutase from human erythrocytes", "journal": "J. Biol. Chem.", "year": 1968, "url": "https://pubmed.ncbi.nlm.nih.gov/5687724/" }, { "id": "PMID:4555506", "title": "X-ray diffraction study of binding of 2,3-diphosphoglycerate to human deoxyhaemoglobin", "journal": "Nature", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/4555506/" } ], "x": 868.6666666666667, "y": 384.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799178", "label": "Intermediate 1 binds HP subcomplex to form Intermediate 2", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799178", "summary": "Intermediate 1 binds HP subcomplex to form Intermediate 2 is a Reactome release 97 curated reaction event. The source record connects HP subcomplex, Intermediate 1 to Intermediate 2 in mitochondrial inner membrane.", "equation": "HP subcomplex + Intermediate 1 → Intermediate 2", "inputs": [ { "node_id": "R-HSA-163929", "reactome_id": "R-HSA-163929", "label": "HP subcomplex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-6788529", "reactome_id": "R-HSA-6788529", "label": "Intermediate 1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6799181", "reactome_id": "R-HSA-6799181", "label": "Intermediate 2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" }, { "id": "PMID:20552642", "title": "Assembly factors of human mitochondrial complex I and their defects in disease", "journal": "IUBMB Life", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20552642/" }, { "id": "PMID:31666358", "title": "The Mitochondrial Acyl-carrier Protein Interaction Network Highlights Important Roles for LYRM Family Members in Complex I and Mitoribosome Assembly", "journal": "Mol Cell Proteomics", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/31666358/" } ], "x": 4730, "y": 910.5666666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799179", "label": "Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799179", "summary": "Peripheral arm subunits bind the 815kDa complex to form a 980kDa complex is a Reactome release 97 curated reaction event. The source record connects 815kDa complex, FP subcomplex, NDUFS5 to 980kDa complex in mitochondrial inner membrane.", "equation": "815kDa complex + FP subcomplex + NDUFS5 → 980kDa complex", "inputs": [ { "node_id": "R-HSA-6799187", "reactome_id": "R-HSA-6799187", "label": "815kDa complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-5689686", "reactome_id": "R-HSA-5689686", "label": "FP subcomplex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-60226", "reactome_id": "R-HSA-60226", "label": "NDUFS5", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6799183", "reactome_id": "R-HSA-6799183", "label": "980kDa complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:21924235", "title": "Understanding mitochondrial complex I assembly in health and disease", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/21924235/" } ], "x": 5008.666666666667, "y": 910.5666666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799191", "label": "Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799191", "summary": "Intermediate 2 binds MT-ND1:NDUFAF5:NDUFAF6 to form a 315kDa subcomplex is a Reactome release 97 curated reaction event. The source record connects Intermediate 2, MT-ND1, NDUFAF5 to 315kDa subcomplex in mitochondrial inner membrane.", "equation": "Intermediate 2 + MT-ND1 + NDUFAF5 + NDUFAF6 → 315kDa subcomplex", "inputs": [ { "node_id": "R-HSA-6799181", "reactome_id": "R-HSA-6799181", "label": "Intermediate 2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-60551", "reactome_id": "R-HSA-60551", "label": "MT-ND1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689127", "reactome_id": "R-HSA-5689127", "label": "NDUFAF5", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689130", "reactome_id": "R-HSA-5689130", "label": "NDUFAF6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6799189", "reactome_id": "R-HSA-6799189", "label": "315kDa subcomplex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" }, { "id": "PMID:20552642", "title": "Assembly factors of human mitochondrial complex I and their defects in disease", "journal": "IUBMB Life", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20552642/" }, { "id": "PMID:27499296", "title": "Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function", "journal": "Mol Cell", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27499296/" }, { "id": "PMID:37159021", "title": "A two-step mitochondrial import pathway couples the disulfide relay with matrix complex I biogenesis", "journal": "J Cell Biol", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37159021/" }, { "id": "PMID:31866046", "title": "Pathogenic Bi-allelic Mutations in NDUFAF8 Cause Leigh Syndrome with an Isolated Complex I Deficiency", "journal": "Am J Hum Genet", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/31866046/" }, { "id": "PMID:35614220", "title": "Defining mitochondrial protein functions through deep multiomic profiling", "journal": "Nature", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35614220/" } ], "x": 4451.333333333333, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799196", "label": "The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799196", "summary": "The MCIA complex, NDUFAF2-7 all dissociate from the 980kDa complex, resulting in Complex I is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 980kDa complex to Complex I, MCIA complex, NDUFAF2 in mitochondrial inner membrane.", "equation": "980kDa complex → Complex I + MCIA complex + NDUFAF2 + NDUFAF3 + NDUFAF4 + NDUFAF5 + NDUFAF6 + NDUFAF7 + TIMMDC1", "inputs": [ { "node_id": "R-HSA-6799183", "reactome_id": "R-HSA-6799183", "label": "980kDa complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6799192", "reactome_id": "R-HSA-6799192", "label": "Complex I", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-5689052", "reactome_id": "R-HSA-5689052", "label": "MCIA complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-5689129", "reactome_id": "R-HSA-5689129", "label": "NDUFAF2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689126", "reactome_id": "R-HSA-5689126", "label": "NDUFAF3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689124", "reactome_id": "R-HSA-5689124", "label": "NDUFAF4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689127", "reactome_id": "R-HSA-5689127", "label": "NDUFAF5", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689130", "reactome_id": "R-HSA-5689130", "label": "NDUFAF6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6788525", "reactome_id": "R-HSA-6788525", "label": "NDUFAF7", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689720", "reactome_id": "R-HSA-5689720", "label": "TIMMDC1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" }, { "id": "PMID:20552642", "title": "Assembly factors of human mitochondrial complex I and their defects in disease", "journal": "IUBMB Life", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20552642/" } ], "x": 4730, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-6799197", "label": "ND4, ND5 bind the 550kDa complex to form the 815kDa complex", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799197", "summary": "ND4, ND5 bind the 550kDa complex to form the 815kDa complex is a Reactome release 97 curated reaction event. The source record connects 550kDa complex, MT-ND4, MT-ND5 to 815kDa complex in mitochondrial inner membrane.", "equation": "550kDa complex + MT-ND4 + MT-ND5 → 815kDa complex", "inputs": [ { "node_id": "R-HSA-6799176", "reactome_id": "R-HSA-6799176", "label": "550kDa complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-60561", "reactome_id": "R-HSA-60561", "label": "MT-ND4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-60565", "reactome_id": "R-HSA-60565", "label": "MT-ND5", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6799187", "reactome_id": "R-HSA-6799187", "label": "815kDa complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" }, { "id": "PMID:20552642", "title": "Assembly factors of human mitochondrial complex I and their defects in disease", "journal": "IUBMB Life", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20552642/" }, { "id": "PMID:27626371", "title": "Accessory subunits are integral for assembly and function of human mitochondrial complex I", "journal": "Nature", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27626371/" } ], "x": 5008.666666666667, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799199", "label": "COA1:MT-ND2, TMEM186:MT-ND3, MT-ND6, NDUFB6 bind the MCIA complex to form a 370kDa subcomplex", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799199", "summary": "COA1:MT-ND2, TMEM186:MT-ND3, MT-ND6, NDUFB6 bind the MCIA complex to form a 370kDa subcomplex is a Reactome release 97 curated reaction event. The source record connects MCIA complex, MT-ND6, NDUFB6 to 370 kDa subcomplex in mitochondrial inner membrane.", "equation": "MCIA complex + MT-ND6 + NDUFB6 + COA1:MT-ND2 + TMEM186:MT-ND3 → 370 kDa subcomplex", "inputs": [ { "node_id": "R-HSA-5689052", "reactome_id": "R-HSA-5689052", "label": "MCIA complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-60569", "reactome_id": "R-HSA-60569", "label": "MT-ND6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-59938", "reactome_id": "R-HSA-59938", "label": "NDUFB6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9708004", "reactome_id": "R-HSA-9708004", "label": "COA1:MT-ND2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9708002", "reactome_id": "R-HSA-9708002", "label": "TMEM186:MT-ND3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6799190", "reactome_id": "R-HSA-6799190", "label": "370 kDa subcomplex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" }, { "id": "PMID:20552642", "title": "Assembly factors of human mitochondrial complex I and their defects in disease", "journal": "IUBMB Life", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20552642/" }, { "id": "PMID:32320651", "title": "Dissecting the Roles of Mitochondrial Complex I Intermediate Assembly Complex Factors in the Biogenesis of Complex I", "journal": "Cell Rep", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32320651/" }, { "id": "PMID:20858599", "title": "FOXRED1, encoding an FAD-dependent oxidoreductase complex-I-specific molecular chaperone, is mutated in infantile-onset mitochondrial encephalopathy", "journal": "Hum Mol Genet", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20858599/" }, { "id": "PMID:25678554", "title": "Characterization of mitochondrial FOXRED1 in the assembly of respiratory chain complex I", "journal": "Hum Mol Genet", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25678554/" }, { "id": "PMID:35333655", "title": "Sideroflexin 4 is a complex I assembly factor that interacts with the MCIA complex and is required for the assembly of the ND2 module", "journal": "Proc Natl Acad Sci U S A", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35333655/" } ], "x": 4451.333333333333, "y": 962.4333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799202", "label": "The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799202", "summary": "The 315kDa subcomplex binds the 370kDa subcomplex to form the 550kDa complex is a Reactome release 97 curated reaction event. The source record connects 315kDa subcomplex, 370 kDa subcomplex to 550kDa complex in mitochondrial inner membrane.", "equation": "315kDa subcomplex + 370 kDa subcomplex → 550kDa complex", "inputs": [ { "node_id": "R-HSA-6799189", "reactome_id": "R-HSA-6799189", "label": "315kDa subcomplex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-6799190", "reactome_id": "R-HSA-6799190", "label": "370 kDa subcomplex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6799176", "reactome_id": "R-HSA-6799176", "label": "550kDa complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" }, { "id": "PMID:20552642", "title": "Assembly factors of human mitochondrial complex I and their defects in disease", "journal": "IUBMB Life", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20552642/" } ], "x": 4730, "y": 962.4333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799203", "label": "IP subcomplex binds NDUFAF3, NDUFAF4, TIMMDC1 to form Intermediate 1", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799203", "summary": "IP subcomplex binds NDUFAF3, NDUFAF4, TIMMDC1 to form Intermediate 1 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects IP subcomplex, NDUFAF3, NDUFAF4 to Intermediate 1 in mitochondrial matrix · mitochondrial inner membrane.", "equation": "IP subcomplex + NDUFAF3 + NDUFAF4 + TIMMDC1 → Intermediate 1", "inputs": [ { "node_id": "R-HSA-5689699", "reactome_id": "R-HSA-5689699", "label": "IP subcomplex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-5689126", "reactome_id": "R-HSA-5689126", "label": "NDUFAF3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689124", "reactome_id": "R-HSA-5689124", "label": "NDUFAF4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689720", "reactome_id": "R-HSA-5689720", "label": "TIMMDC1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6788529", "reactome_id": "R-HSA-6788529", "label": "Intermediate 1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" }, { "id": "PMID:20552642", "title": "Assembly factors of human mitochondrial complex I and their defects in disease", "journal": "IUBMB Life", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20552642/" }, { "id": "PMID:20406883", "title": "MidA is a putative methyltransferase that is required for mitochondrial complex I function", "journal": "J. Cell. Sci.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20406883/" }, { "id": "PMID:21924235", "title": "Understanding mitochondrial complex I assembly in health and disease", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/21924235/" }, { "id": "PMID:11220739", "title": "Mutations in the complex I NDUFS2 gene of patients with cardiomyopathy and encephalomyopathy", "journal": "Ann. Neurol.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11220739/" } ], "x": 5008.666666666667, "y": 962.4333333333334, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-6799495", "label": "GLYCTK phosphorylates DGA to 3PDGA", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "fructose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23517" ], "source_url": "https://reactome.org/content/detail/R-HSA-6799495", "summary": "GLYCTK phosphorylates DGA to 3PDGA is a Reactome release 97 curated reaction event. The source record connects DGA, ATP to 3PDGA, ADP, H+ in cytosol and cross-references RHEA:23517.", "equation": "DGA + ATP → 3PDGA + ADP + H+", "inputs": [ { "node_id": "R-ALL-6799497", "reactome_id": "R-ALL-6799497", "label": "DGA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16659", "formula": "C3H5O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6799493", "reactome_id": "R-ALL-6799493", "label": "3PDGA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58272", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycerate kinase activity of GLYCTK [cytosol]", "activity_id": "6799496", "ec_numbers": [ "2.7.1.31" ], "accessions": [ "Q8IVS8" ] } ], "citations": [ { "id": "PMID:16753811", "title": "Isolation and characterization of the human D-glyceric acidemia related glycerate kinase gene GLYCTK1 and its alternatively splicing variant GLYCTK2", "journal": "DNA Seq.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16753811/" }, { "id": "PMID:2537226", "title": "D-glycerate kinase deficiency as a cause of D-glyceric aciduria", "journal": "FEBS Lett.", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2537226/" }, { "id": "PMID:20949620", "title": "D-glyceric aciduria is caused by genetic deficiency of D-glycerate kinase (GLYCTK)", "journal": "Hum. Mutat.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20949620/" } ], "x": 2381.3333333333335, "y": 457.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799545", "label": "MAN2C1 hydrolyses GlcNAc (Man)9 to GlcNAc (Man)5", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "oligosaccharide-recovery" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799545", "summary": "MAN2C1 hydrolyses GlcNAc (Man)9 to GlcNAc (Man)5 is a Reactome release 97 curated reaction event. The source record connects alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-bet…, H2O to alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc, Man in cytosol.", "equation": "alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-bet… + 4 H2O → alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc + 4 Man", "inputs": [ { "node_id": "R-ALL-6799540", "reactome_id": "R-ALL-6799540", "label": "alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e2)-alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-bet…", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:91284", "formula": "C62H105NO51", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 4, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6799543", "reactome_id": "R-ALL-6799543", "label": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:91280", "formula": "C38H65NO31", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-429579", "reactome_id": "R-ALL-429579", "label": "Man", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:4208", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alpha-mannosidase activity of MAN2C1:Zn2+ [cytosol]", "activity_id": "6799539", "ec_numbers": [ "3.2.1.24" ], "accessions": [] } ], "citations": [ { "id": "PMID:16848760", "title": "Man2C1, an alpha-mannosidase, is involved in the trimming of free oligosaccharides in the cytosol", "journal": "Biochem. J.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16848760/" } ], "x": 3321.3333333333335, "y": 839.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799581", "label": "MAN2B2 hydrolyses GlcNAc (Man)3 to GlcNAc:Man", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "oligosaccharide-recovery" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6799581", "summary": "MAN2B2 hydrolyses GlcNAc (Man)3 to GlcNAc:Man is a Reactome release 97 curated reaction event. The source record connects alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc, H2O to GlcNAc:Man, Man in lysosomal lumen.", "equation": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc + H2O → GlcNAc:Man + 2 Man", "inputs": [ { "node_id": "R-ALL-6799577", "reactome_id": "R-ALL-6799577", "label": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:91279", "formula": "C26H45NO21", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8853719", "reactome_id": "R-ALL-8853719", "label": "GlcNAc:Man", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:71553", "formula": "C14H25NO11", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-6799594", "reactome_id": "R-ALL-6799594", "label": "Man", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:4208", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "mannan endo-1,6-alpha-mannosidase activity of MAN2B2:Zn2+ [lysosomal lumen]", "activity_id": "6799586", "ec_numbers": [ "3.2.1.101" ], "accessions": [] } ], "citations": [ { "id": "PMID:19722277", "title": "Human lysosomal alpha-mannosidases exhibit different inhibition and metal binding properties", "journal": "Protein Sci.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19722277/" }, { "id": "PMID:16115860", "title": "Characterization of a human core-specific lysosomal {alpha}1,6-mannosidase involved in N-glycan catabolism", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16115860/" } ], "x": 3600, "y": 839.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6799604", "label": "GNPDA1,2 hexamers deaminate GlcN6P to Fru(6)P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12173" ], "source_url": "https://reactome.org/content/detail/R-HSA-6799604", "summary": "GNPDA1,2 hexamers deaminate GlcN6P to Fru(6)P is a Reactome release 97 curated reaction event. The source record connects GlcN6P, H2O to Fru(6)P, NH4+ in cytosol and cross-references RHEA:12173.", "equation": "GlcN6P + H2O → Fru(6)P + NH4+", "inputs": [ { "node_id": "R-ALL-449701", "reactome_id": "R-ALL-449701", "label": "GlcN6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:75989", "formula": "C6H13NO8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucosamine-6-phosphate deaminase activity of GNPDA1,2 hexamers [cytosol]", "activity_id": "6799599", "ec_numbers": [ "3.5.99.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:9438414", "title": "Molecularly cloned mammalian glucosamine-6-phosphate deaminase localizes to transporting epithelium and lacks oscillin activity", "journal": "FASEB J.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9438414/" }, { "id": "PMID:12616532", "title": "Cloning and functional characterization of GNPI2, a novel human homolog of glucosamine-6-phosphate isomerase/oscillin", "journal": "J. Cell. Biochem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12616532/" }, { "id": "PMID:12965206", "title": "Two mammalian glucosamine-6-phosphate deaminases: a structural and genetic study", "journal": "FEBS Lett.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12965206/" } ], "x": 868.6666666666667, "y": 335.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6800052", "label": "SREBP1A,1C binds the SCD gene", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6800052", "summary": "SREBP1A,1C binds the SCD gene is a Reactome release 97 curated reaction event. The source record connects SCD gene, SREBP1A,1C dimer to SREBP1A,1C:SCD gene in nucleoplasm.", "equation": "SCD gene + SREBP1A,1C dimer → SREBP1A,1C:SCD gene", "inputs": [ { "node_id": "R-HSA-6800051", "reactome_id": "R-HSA-6800051", "label": "SCD gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2065537", "reactome_id": "R-HSA-2065537", "label": "SREBP1A,1C dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1655743", "reactome_id": "R-HSA-1655743", "label": "SREBP1A,1C:SCD gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of SREBP1A,1C dimer [nucleoplasm]", "activity_id": "9987248", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18654640", "title": "Genome-wide occupancy of SREBP1 and its partners NFY and SP1 reveals novel functional roles and combinatorial regulation of distinct classes of genes", "journal": "PLoS Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18654640/" }, { "id": "PMID:11414710", "title": "Cloning and characterization of the human stearoyl-CoA desaturase gene promoter: transcriptional activation by sterol regulatory element binding protein and repression by polyunsaturated fatty acids and cholesterol", "journal": "Biochem Biophys Res Commun", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11414710/" }, { "id": "PMID:10400691", "title": "Identification of conserved cis-elements and transcription factors required for sterol-regulated transcription of stearoyl-CoA desaturase 1 and 2", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10400691/" }, { "id": "PMID:11994399", "title": "SREBPs: activators of the complete program of cholesterol and fatty acid synthesis in the liver", "journal": "J Clin Invest", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11994399/" }, { "id": "PMID:10585467", "title": "Sterol regulatory element-binding protein-1 as a key transcription factor for nutritional induction of lipogenic enzyme genes", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10585467/" } ], "x": 4730, "y": 1812.9621212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6800149", "label": "N6AMT1:TRMT112 transfers CH3 group from AdoMet to MMAIII", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11685" ], "source_url": "https://reactome.org/content/detail/R-HSA-6800149", "summary": "N6AMT1:TRMT112 transfers CH3 group from AdoMet to MMAIII is a Reactome release 97 curated reaction event. The source record connects MMAIII, AdoMet to DMAA, AdoHcy in cytosol and cross-references RHEA:11685.", "equation": "MMAIII + AdoMet → DMAA + AdoHcy", "inputs": [ { "node_id": "R-ALL-6806829", "reactome_id": "R-ALL-6806829", "label": "MMAIII", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17826", "formula": "CH5AsO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6800127", "reactome_id": "R-ALL-6800127", "label": "DMAA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:48765", "formula": "C2H7AsO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methyltransferase activity of N6AMT1:TRMT112 [cytosol]", "activity_id": "6800155", "ec_numbers": [ "2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:21193388", "title": "Involvement of N-6 adenine-specific DNA methyltransferase 1 (N6AMT1) in arsenic biomethylation and its role in arsenic-induced toxicity", "journal": "Environ. Health Perspect.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21193388/" }, { "id": "PMID:18539146", "title": "HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1", "journal": "FEBS Lett.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18539146/" } ], "x": 8018.666666666667, "y": 2921.225490196078, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6800334", "label": "MOGAT1,2,3 transfer acyl group from acyl-CoA to 2-acylglycerol to form DAG", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:32948" ], "source_url": "https://reactome.org/content/detail/R-HSA-6800334", "summary": "MOGAT1,2,3 transfer acyl group from acyl-CoA to 2-acylglycerol to form DAG is a Reactome release 97 curated reaction event. The source record connects 2AG, acyl-CoA to DAG, CoA-SH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:32948.", "equation": "2AG + acyl-CoA → DAG + CoA-SH", "inputs": [ { "node_id": "R-ALL-6800352", "reactome_id": "R-ALL-6800352", "label": "2AG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17389", "formula": "C4H7O4R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76103", "reactome_id": "R-ALL-76103", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "2-acylglycerol O-acyltransferase activity of MOGAT1,2,3 [endoplasmic reticulum membrane]", "activity_id": "6800350", "ec_numbers": [ "2.3.1.22" ], "accessions": [] } ], "citations": [ { "id": "PMID:12618427", "title": "Identification of acyl coenzyme A:monoacylglycerol acyltransferase 3, an intestinal specific enzyme implicated in dietary fat absorption", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12618427/" }, { "id": "PMID:12824082", "title": "Human intestinal monoacylglycerol acyltransferase: differential features in tissue expression and activity", "journal": "Am. J. Physiol. Endocrinol. Metab.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12824082/" }, { "id": "PMID:14970677", "title": "Genomic organization of the DGAT2/MOGAT gene family in cattle (Bos taurus) and other mammals", "journal": "Cytogenet. Genome Res.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14970677/" }, { "id": "PMID:25838202", "title": "Monoacylglycerol O-acyltransferase 1 is regulated by peroxisome proliferator-activated receptor γ in human hepatocytes and increases lipid accumulation", "journal": "Biochem. Biophys. Res. Commun.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25838202/" } ], "x": 3600, "y": 2133.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6800868", "label": "NDUF subunits bind to form the IP subcomplex", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6800868", "summary": "NDUF subunits bind to form the IP subcomplex is a Reactome release 97 curated reaction event. The source record connects NDUFA9:FAD, NDUFAF7:NDUFS2:2x4Fe-4S, NDUFS3 to IP subcomplex in mitochondrial matrix.", "equation": "NDUFA9:FAD + NDUFAF7:NDUFS2:2x4Fe-4S + NDUFS3 + NDUFS7:4Fe-4S + NDUFS8:2x4Fe-4S → IP subcomplex", "inputs": [ { "node_id": "R-HSA-164289", "reactome_id": "R-HSA-164289", "label": "NDUFA9:FAD", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-164288", "reactome_id": "R-HSA-164288", "label": "NDUFAF7:NDUFS2:2x4Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-163927", "reactome_id": "R-HSA-163927", "label": "NDUFS3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-164293", "reactome_id": "R-HSA-164293", "label": "NDUFS7:4Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-164295", "reactome_id": "R-HSA-164295", "label": "NDUFS8:2x4Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5689699", "reactome_id": "R-HSA-5689699", "label": "IP subcomplex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20552642", "title": "Assembly factors of human mitochondrial complex I and their defects in disease", "journal": "IUBMB Life", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20552642/" }, { "id": "PMID:24191001", "title": "Assembly factors for the membrane arm of human complex I", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24191001/" } ], "x": 4451.333333333333, "y": 988.3666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6800870", "label": "NDUF subunits bind to form the FP subcomplex", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6800870", "summary": "NDUF subunits bind to form the FP subcomplex is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects NDUFA12, NDUFAF2, NDUFS1:2x4Fe-4S to FP subcomplex in mitochondrial inner membrane · mitochondrial matrix.", "equation": "NDUFA12 + NDUFAF2 + NDUFS1:2x4Fe-4S + NDUFS4 + NDUFS6 + NDUFV1:4Fe-4S:FMN + NDUFV2:4Fe-4S + NDUFV3 → FP subcomplex", "inputs": [ { "node_id": "R-HSA-59864", "reactome_id": "R-HSA-59864", "label": "NDUFA12", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-5689129", "reactome_id": "R-HSA-5689129", "label": "NDUFAF2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-164297", "reactome_id": "R-HSA-164297", "label": "NDUFS1:2x4Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-163946", "reactome_id": "R-HSA-163946", "label": "NDUFS4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-163922", "reactome_id": "R-HSA-163922", "label": "NDUFS6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6788516", "reactome_id": "R-HSA-6788516", "label": "NDUFV1:4Fe-4S:FMN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-6788524", "reactome_id": "R-HSA-6788524", "label": "NDUFV2:4Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-163943", "reactome_id": "R-HSA-163943", "label": "NDUFV3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5689686", "reactome_id": "R-HSA-5689686", "label": "FP subcomplex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:21924235", "title": "Understanding mitochondrial complex I assembly in health and disease", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/21924235/" } ], "x": 4730, "y": 988.3666666666667, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-6801328", "label": "MTHFD2, D2L oxidise 5,10-methylene-THF to 5,10-methenyl-THF", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:22893" ], "source_url": "https://reactome.org/content/detail/R-HSA-6801328", "summary": "MTHFD2, D2L oxidise 5,10-methylene-THF to 5,10-methenyl-THF is a Reactome release 97 curated reaction event. The source record connects 5,10-methylene-THF, NAD+ to 5,10-methenyl-THF, NADH in mitochondrial matrix and cross-references RHEA:22893.", "equation": "5,10-methylene-THF + NAD+ → 5,10-methenyl-THF + NADH", "inputs": [ { "node_id": "R-ALL-5693984", "reactome_id": "R-ALL-5693984", "label": "5,10-methylene-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15636", "formula": "C20H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6801458", "reactome_id": "R-ALL-6801458", "label": "5,10-methenyl-THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57455", "formula": "C20H20N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "methenyltetrahydrofolate cyclohydrolase activity of MTHFD2, D2L [mitochondrial matrix]", "activity_id": "6801460", "ec_numbers": [ "3.5.4.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:24733394", "title": "Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues", "journal": "J. Biol. Chem.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24733394/" }, { "id": "PMID:21163947", "title": "Mammalian MTHFD2L encodes a mitochondrial methylenetetrahydrofolate dehydrogenase isozyme expressed in adult tissues", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21163947/" }, { "id": "PMID:8218174", "title": "NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is the mammalian homolog of the mitochondrial enzyme encoded by the yeast MIS1 gene", "journal": "Biochemistry", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8218174/" } ], "x": 4451.333333333333, "y": 2891.409090909091, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6801342", "label": "MTHFS transforms 5-formyl-THFPG to 5,10-methenyl-THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6801342", "summary": "MTHFS transforms 5-formyl-THFPG to 5,10-methenyl-THFPG is a Reactome release 97 curated reaction event. The source record connects 5-formyl-THFPG, ATP to 5,10-methenyl-THFPG, ADP, Pi in cytosol.", "equation": "5-formyl-THFPG + ATP → 5,10-methenyl-THFPG + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-6803257", "reactome_id": "R-ALL-6803257", "label": "5-formyl-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63907", "formula": "(C5H7NO3)n.C25H32N8O9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-200640", "reactome_id": "R-ALL-200640", "label": "5,10-methenyl-THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65049", "formula": "(C5H6NO3)n.C25H26N8O9", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "5-formyltetrahydrofolate cyclo-ligase activity of MTHFS [cytosol]", "activity_id": "6801334", "ec_numbers": [ "6.3.3.2" ], "accessions": [ "P49914" ] } ], "citations": [ { "id": "PMID:8522195", "title": "Cloning and characterization of the human 5,10-methenyltetrahydrofolate synthetase-encoding cDNA", "journal": "Gene", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8522195/" }, { "id": "PMID:3801490", "title": "Human liver methenyltetrahydrofolate synthetase: improved purification and increased affinity for folate polyglutamate substrates", "journal": "Biochim. Biophys. Acta", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3801490/" } ], "x": 4451.333333333333, "y": 2997.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6801456", "label": "MTHFD1L transforms 10-formyl-THF to HCOOH and THF", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6801456", "summary": "MTHFD1L transforms 10-formyl-THF to HCOOH and THF is a Reactome release 97 curated reaction event. The source record connects 10-formyl-THF, Pi, ADP to HCOOH, THF, ATP in mitochondrial matrix.", "equation": "10-formyl-THF + Pi + ADP → HCOOH + THF + ATP", "inputs": [ { "node_id": "R-ALL-5389850", "reactome_id": "R-ALL-5389850", "label": "10-formyl-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195366", "formula": "C20H21N7O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6801451", "reactome_id": "R-ALL-6801451", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-200671", "reactome_id": "R-ALL-200671", "label": "THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "formate-tetrahydrofolate ligase activity of MTHFD1L dimer [mitochondrial matrix]", "activity_id": "5696827", "ec_numbers": [ "6.3.4.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:12937168", "title": "Human mitochondrial C1-tetrahydrofolate synthase: gene structure, tissue distribution of the mRNA, and immunolocalization in Chinese hamster ovary calls", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12937168/" }, { "id": "PMID:16171773", "title": "Enzymatic characterization of human mitochondrial C1-tetrahydrofolate synthase", "journal": "Arch. Biochem. Biophys.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16171773/" }, { "id": "PMID:19948730", "title": "Mitochondrial C1-tetrahydrofolate synthase (MTHFD1L) supports the flow of mitochondrial one-carbon units into the methyl cycle in embryos", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19948730/" } ], "x": 4730, "y": 2997.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6801462", "label": "MTHFD2, D2L oxidise 5,10-methenyl-THF to 10-formyl-THF", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6801462", "summary": "MTHFD2, D2L oxidise 5,10-methenyl-THF to 10-formyl-THF is a Reactome release 97 curated reaction event. The source record connects 5,10-methenyl-THF, H2O to 10-formyl-THF in mitochondrial matrix.", "equation": "5,10-methenyl-THF + H2O → 10-formyl-THF", "inputs": [ { "node_id": "R-ALL-6801458", "reactome_id": "R-ALL-6801458", "label": "5,10-methenyl-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57455", "formula": "C20H20N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5389850", "reactome_id": "R-ALL-5389850", "label": "10-formyl-THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195366", "formula": "C20H21N7O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methylenetetrahydrofolate dehydrogenase (NAD+) activity of MTHFD2, D2L [mitochondrial matrix]", "activity_id": "6801459", "ec_numbers": [ "1.5.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:21163947", "title": "Mammalian MTHFD2L encodes a mitochondrial methylenetetrahydrofolate dehydrogenase isozyme expressed in adult tissues", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21163947/" }, { "id": "PMID:24733394", "title": "Mitochondrial MTHFD2L is a dual redox cofactor-specific methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase expressed in both adult and embryonic tissues", "journal": "J. Biol. Chem.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24733394/" }, { "id": "PMID:8218174", "title": "NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase is the mammalian homolog of the mitochondrial enzyme encoded by the yeast MIS1 gene", "journal": "Biochemistry", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8218174/" } ], "x": 4451.333333333333, "y": 3103.590909090909, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6803255", "label": "HCOOH translocated from mitochondrial matrix to cytosol", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [ "RHEA:29679" ], "source_url": "https://reactome.org/content/detail/R-HSA-6803255", "summary": "HCOOH translocated from mitochondrial matrix to cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects HCOOH to HCOOH in cytosol · mitochondrial matrix and cross-references RHEA:29679.", "equation": "HCOOH → HCOOH", "inputs": [ { "node_id": "R-ALL-6801451", "reactome_id": "R-ALL-6801451", "label": "HCOOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20645850", "title": "Compartmentalization of Mammalian folate-mediated one-carbon metabolism", "journal": "Annu. Rev. Nutr.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20645850/" } ], "x": 4730, "y": 2891.409090909091, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-6806877", "label": "NME1:NME3 heterohexamer phosphorylates NDPs to NTPs", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18114" ], "source_url": "https://reactome.org/content/detail/R-HSA-6806877", "summary": "NME1:NME3 heterohexamer phosphorylates NDPs to NTPs is a Reactome release 97 curated reaction event. The source record connects ATP, NDP(3-) to ADP, NTP in cytosol and cross-references RHEA:18114.", "equation": "ATP + NDP(3-) → ADP + NTP", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8866401", "reactome_id": "R-ALL-8866401", "label": "NDP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-6806881", "reactome_id": "R-ALL-6806881", "label": "NTP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61557", "formula": "C5H8O13P3R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nucleoside diphosphate kinase activity of NME1:NME3 heterohexamer [cytosol]", "activity_id": "6806878", "ec_numbers": [ "2.7.4.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:11277919", "title": "Structural and catalytic properties and homology modelling of the human nucleoside diphosphate kinase C, product of the DRnm23 gene", "journal": "Eur J Biochem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11277919/" } ], "x": 3321.3333333333335, "y": 3135.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6806966", "label": "APOA1BP dimer epimerises R-NAD(P)HX to S-NAD(P)HX", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:32216" ], "source_url": "https://reactome.org/content/detail/R-HSA-6806966", "summary": "APOA1BP dimer epimerises R-NAD(P)HX to S-NAD(P)HX is a Reactome release 97 curated reaction event. The source record connects R-NADPHX to S-NADPHX in mitochondrial matrix and cross-references RHEA:32216.", "equation": "R-NADPHX → S-NADPHX", "inputs": [ { "node_id": "R-ALL-6807473", "reactome_id": "R-ALL-6807473", "label": "R-NADPHX", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64075", "formula": "C21H29N7O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6807477", "reactome_id": "R-ALL-6807477", "label": "S-NADPHX", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64074", "formula": "C21H29N7O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "NAD(P)HX epimerase activity of APOA1BP dimer [mitochondrial matrix]", "activity_id": "6807480", "ec_numbers": [ "5.1.99.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:11991719", "title": "Cloning and characterization of a novel apolipoprotein A-I binding protein, AI-BP, secreted by cells of the kidney proximal tubules in response to HDL or ApoA-I", "journal": "Genomics", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11991719/" }, { "id": "PMID:21994945", "title": "Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21994945/" } ], "x": 5391.333333333333, "y": 2879.7763157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6806967", "label": "CARKD dehydrates S-NAD(P)HX to NADPH", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:32232" ], "source_url": "https://reactome.org/content/detail/R-HSA-6806967", "summary": "CARKD dehydrates S-NAD(P)HX to NADPH is a Reactome release 97 curated reaction event. The source record connects S-NADPHX, ATP to NADPH, ADP, Pi in mitochondrial matrix and cross-references RHEA:32232.", "equation": "S-NADPHX + ATP → NADPH + ADP + Pi + 3 H+", "inputs": [ { "node_id": "R-ALL-6807477", "reactome_id": "R-ALL-6807477", "label": "S-NADPHX", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64074", "formula": "C21H29N7O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ATP-dependent NAD(P)H-hydrate dehydratase activity of CARKD [mitochondrial matrix]", "activity_id": "8847933", "ec_numbers": [ "4.2.1.93" ], "accessions": [ "Q8IW45" ] } ], "citations": [ { "id": "PMID:21994945", "title": "Extremely conserved ATP- or ADP-dependent enzymatic system for nicotinamide nucleotide repair", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21994945/" } ], "x": 5391.333333333333, "y": 2900.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6807052", "label": "EBP isomerizes ZYMSTNL to LTHSOL", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005789" ], "rhea_ids": [ "RHEA:15283" ], "source_url": "https://reactome.org/content/detail/R-HSA-6807052", "summary": "EBP isomerizes ZYMSTNL to LTHSOL is a Reactome release 97 curated reaction event. The source record connects ZYMSTNL to LTHSOL in endoplasmic reticulum membrane and cross-references RHEA:15283.", "equation": "ZYMSTNL → LTHSOL", "inputs": [ { "node_id": "R-ALL-6807048", "reactome_id": "R-ALL-6807048", "label": "ZYMSTNL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16608", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6807054", "reactome_id": "R-ALL-6807054", "label": "LTHSOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17168", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "steroid Delta-isomerase activity of EBP [endoplasmic reticulum membrane]", "activity_id": "195674", "ec_numbers": [ "5.3.3.1" ], "accessions": [ "Q15125" ] } ], "citations": [ { "id": "PMID:10391219", "title": "Mutations in the gene encoding 3 beta-hydroxysteroid-delta 8, delta 7-isomerase cause X-linked dominant Conradi-Hunermann syndrome", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10391219/" }, { "id": "PMID:10391218", "title": "Mutations in a delta 8-delta 7 sterol isomerase in the tattered mouse and X-linked dominant chondrodysplasia punctata. jderry@immunex.com.", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10391218/" }, { "id": "PMID:14404284", "title": "Preputial gland tumor sterols. 3. A metabolic pathway from lanosterol to cholesterol", "journal": "J. Biol. Chem.", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/14404284/" }, { "id": "PMID:26114596", "title": "Flux analysis of cholesterol biosynthesis in vivo reveals multiple tissue and cell-type specific pathways", "journal": "Elife", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26114596/" } ], "x": 2660, "y": 2284.3, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6807053", "label": "SC5D desaturates LTHSOL to 7-dehydroCHOL", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:46557" ], "source_url": "https://reactome.org/content/detail/R-HSA-6807053", "summary": "SC5D desaturates LTHSOL to 7-dehydroCHOL is a Reactome release 97 curated reaction event. The source record connects LTHSOL, H+, O2 to 7-dehydroCHOL, H2O, CYP450:Fe3+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:46557.", "equation": "LTHSOL + 2 H+ + O2 + 2 CYP450:Fe2+ → 7-dehydroCHOL + 2 H2O + 2 CYP450:Fe3+", "inputs": [ { "node_id": "R-ALL-6807054", "reactome_id": "R-ALL-6807054", "label": "LTHSOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17168", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-8957354", "reactome_id": "R-HSA-8957354", "label": "CYP450:Fe2+", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6807057", "reactome_id": "R-ALL-6807057", "label": "7-dehydroCHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17759", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-8957351", "reactome_id": "R-HSA-8957351", "label": "CYP450:Fe3+", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "C-5 sterol desaturase activity of SC5D [endoplasmic reticulum membrane]", "activity_id": "195666", "ec_numbers": null, "accessions": [ "O75845" ] } ], "citations": [ { "id": "PMID:12189593", "title": "Lathosterolosis, a novel multiple-malformation/mental retardation syndrome due to deficiency of 3beta-hydroxysteroid-delta5-desaturase", "journal": "Am J Hum Genet", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12189593/" }, { "id": "PMID:14404284", "title": "Preputial gland tumor sterols. 3. A metabolic pathway from lanosterol to cholesterol", "journal": "J. Biol. Chem.", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/14404284/" }, { "id": "PMID:12812989", "title": "Lathosterolosis: an inborn error of human and murine cholesterol synthesis due to lathosterol 5-desaturase deficiency", "journal": "Hum Mol Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12812989/" }, { "id": "PMID:26114596", "title": "Flux analysis of cholesterol biosynthesis in vivo reveals multiple tissue and cell-type specific pathways", "journal": "Elife", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26114596/" } ], "x": 2660, "y": 2362.1, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6807055", "label": "DHCR7 reduces 7-dehydroCHOL to CHOL", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:23984", "RHEA:23986" ], "source_url": "https://reactome.org/content/detail/R-HSA-6807055", "summary": "DHCR7 reduces 7-dehydroCHOL to CHOL is a Reactome release 97 curated reaction event. The source record connects 7-dehydroCHOL, NADPH, H+ to CHOL, NADP+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:23984, RHEA:23986.", "equation": "7-dehydroCHOL + NADPH + H+ → CHOL + NADP+", "inputs": [ { "node_id": "R-ALL-6807057", "reactome_id": "R-ALL-6807057", "label": "7-dehydroCHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17759", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193384", "reactome_id": "R-ALL-193384", "label": "CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "7-dehydrocholesterol reductase activity of DHCR7 [endoplasmic reticulum membrane]", "activity_id": "196403", "ec_numbers": [ "1.3.1.21" ], "accessions": [ "Q9UBM7" ] } ], "citations": [ { "id": "PMID:14404284", "title": "Preputial gland tumor sterols. 3. A metabolic pathway from lanosterol to cholesterol", "journal": "J. Biol. Chem.", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/14404284/" }, { "id": "PMID:26114596", "title": "Flux analysis of cholesterol biosynthesis in vivo reveals multiple tissue and cell-type specific pathways", "journal": "Elife", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26114596/" }, { "id": "PMID:9465114", "title": "Molecular cloning and expression of the human delta7-sterol reductase", "journal": "Proc Natl Acad Sci U S A", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9465114/" } ], "x": 2938.666666666667, "y": 2102.766666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6807064", "label": "DHCR24 reduces ZYMOL to ZYMSTNL", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:36399", "RHEA:36401" ], "source_url": "https://reactome.org/content/detail/R-HSA-6807064", "summary": "DHCR24 reduces ZYMOL to ZYMSTNL is a Reactome release 97 curated reaction event. The source record connects ZYMOL, NADPH, H+ to ZYMSTNL, NADP+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:36399, RHEA:36401.", "equation": "ZYMOL + NADPH + H+ → ZYMSTNL + NADP+", "inputs": [ { "node_id": "R-ALL-194691", "reactome_id": "R-ALL-194691", "label": "ZYMOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18252", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6807048", "reactome_id": "R-ALL-6807048", "label": "ZYMSTNL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16608", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "Delta24-sterol reductase activity of DHCR24 [endoplasmic reticulum membrane]", "activity_id": "196404", "ec_numbers": [ "1.3.1.72" ], "accessions": [ "Q15392" ] } ], "citations": [ { "id": "PMID:14404284", "title": "Preputial gland tumor sterols. 3. A metabolic pathway from lanosterol to cholesterol", "journal": "J. Biol. Chem.", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/14404284/" }, { "id": "PMID:26114596", "title": "Flux analysis of cholesterol biosynthesis in vivo reveals multiple tissue and cell-type specific pathways", "journal": "Elife", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26114596/" }, { "id": "PMID:11519011", "title": "Mutations in the 3beta-hydroxysterol Delta24-reductase gene cause desmosterolosis, an autosomal recessive disorder of cholesterol biosynthesis", "journal": "Am J Hum Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11519011/" } ], "x": 2381.3333333333335, "y": 2128.7, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6807242", "label": "25(OH)D translocates from ER membrane to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "endoplasmic reticulum membrane · extracellular region", "compartment_ids": [ "GO:0005789", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6807242", "summary": "25(OH)D translocates from ER membrane to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 25(OH)D to 25(OH)D in endoplasmic reticulum membrane · extracellular region.", "equation": "25(OH)D → 25(OH)D", "inputs": [ { "node_id": "R-ALL-8963879", "reactome_id": "R-ALL-8963879", "label": "25(OH)D", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17933", "formula": "C27H44O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-209906", "reactome_id": "R-ALL-209906", "label": "25(OH)D", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17933", "formula": "C27H44O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11799400", "title": "A structural basis for the unique binding features of the human vitamin D-binding protein", "journal": "Nat Struct Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11799400/" } ], "x": 4730, "y": 1860.1136363636363, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-6807826", "label": "LDHAL6B reduces PYR to LACT", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:23446", "RHEA:23447" ], "source_url": "https://reactome.org/content/detail/R-HSA-6807826", "summary": "LDHAL6B reduces PYR to LACT is a Reactome release 97 curated reaction event. The source record connects PYR, NADH, H+ to LACT, NAD+ in mitochondrial matrix and cross-references RHEA:23446, RHEA:23447.", "equation": "PYR + NADH + H+ → LACT + NAD+", "inputs": [ { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6807616", "reactome_id": "R-ALL-6807616", "label": "LACT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:422", "formula": "C3H6O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-lactate dehydrogenase (NAD+) activity of LDHAL6B [mitochondrial matrix]", "activity_id": "6807609", "ec_numbers": [ "1.1.1.27" ], "accessions": [ "Q9BYZ2" ] } ], "citations": [ { "id": "PMID:19679512", "title": "Computational analyses of mammalian lactate dehydrogenases: human, mouse, opossum and platypus LDHs", "journal": "Comput Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19679512/" }, { "id": "PMID:15870898", "title": "Cloning and characterization of a novel intronless lactate dehydrogenase gene in human testis", "journal": "Int. J. Mol. Med.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15870898/" } ], "x": 4730, "y": 282.45, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6808464", "label": "ALDH3B2 oxidises HXAL to PALM", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lipid droplet · cytosol", "compartment_ids": [ "GO:0005811", "GO:0005829" ], "rhea_ids": [ "RHEA:33739" ], "source_url": "https://reactome.org/content/detail/R-HSA-6808464", "summary": "ALDH3B2 oxidises HXAL to PALM is a Reactome release 97 curated reaction event. The source record connects HXAL, H2O, NAD+ to PALM, NADH, H+ in lipid droplet · cytosol and cross-references RHEA:33739.", "equation": "HXAL + H2O + NAD+ → PALM + NADH + H+", "inputs": [ { "node_id": "R-ALL-6808468", "reactome_id": "R-ALL-6808468", "label": "HXAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17600", "formula": "C16H32O", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2453691", "reactome_id": "R-ALL-2453691", "label": "PALM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:7896", "formula": "C16H31O2", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldehyde dehydrogenase [NAD(P)+] activity of ALDH3B2 [lipid droplet]", "activity_id": "6808465", "ec_numbers": [ "1.2.1.5" ], "accessions": [ "P48448" ] } ], "citations": [ { "id": "PMID:23721920", "title": "Substrate specificity, plasma membrane localization, and lipid modification of the aldehyde dehydrogenase ALDH3B1", "journal": "Biochim. Biophys. Acta", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23721920/" }, { "id": "PMID:25286108", "title": "Mouse aldehyde dehydrogenase ALDH3B2 is localized to lipid droplets via two C-terminal tryptophan residues and lipid modification", "journal": "Biochem. J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25286108/" } ], "x": 3600, "y": 1555.4423076923076, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6808487", "label": "ALDH1L2 dehydrogenates 10-formyl-THFPG to THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6808487", "summary": "ALDH1L2 dehydrogenates 10-formyl-THFPG to THFPG is a Reactome release 97 curated reaction event. The source record connects 10-formyl-THFPG, NADP+, H2O to THFPG, NADPH, H+ in mitochondrial matrix.", "equation": "10-formyl-THFPG + NADP+ + H2O → THFPG + NADPH + H+ + CO2", "inputs": [ { "node_id": "R-ALL-6808499", "reactome_id": "R-ALL-6808499", "label": "10-formyl-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28010", "formula": "(C5H7NO3)n.C25H30N8O10", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-200679", "reactome_id": "R-ALL-200679", "label": "THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28624", "formula": "(C5H7NO3)n.C19H23N7O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of ADH1L2 tetramer [mitochondrial matrix]", "activity_id": "6808492", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:20498374", "title": "ALDH1L2 is the mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20498374/" }, { "id": "PMID:21238436", "title": "Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase", "journal": "Chem. Biol. Interact.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21238436/" } ], "x": 5008.666666666667, "y": 2891.409090909091, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6808496", "label": "ALDH1L1 dehydrogenates 10-formyl-THFPG to THFPG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6808496", "summary": "ALDH1L1 dehydrogenates 10-formyl-THFPG to THFPG is a Reactome release 97 curated reaction event. The source record connects 10-formyl-THFPG, NADP+, H2O to THFPG, NADPH, H+ in cytosol.", "equation": "10-formyl-THFPG + NADP+ + H2O → THFPG + NADPH + H+ + CO2", "inputs": [ { "node_id": "R-ALL-111353", "reactome_id": "R-ALL-111353", "label": "10-formyl-THFPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28010", "formula": "(C5H7NO3)n.C25H30N8O10", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-197969", "reactome_id": "R-ALL-197969", "label": "THFPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28624", "formula": "(C5H7NO3)n.C19H23N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of ALDH1L1 tetramer [cytosol]", "activity_id": "6808489", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:20498374", "title": "ALDH1L2 is the mitochondrial homolog of 10-formyltetrahydrofolate dehydrogenase", "journal": "J. 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The source record connects MTMR7, MTMR9 to MTMR7:MTMR9 in cytosol.", "equation": "MTMR7 + MTMR9 → MTMR7:MTMR9", "inputs": [ { "node_id": "R-HSA-1604650", "reactome_id": "R-HSA-1604650", "label": "MTMR7", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6809239", "reactome_id": "R-HSA-6809239", "label": "MTMR9", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6809241", "reactome_id": "R-HSA-6809241", "label": "MTMR7:MTMR9", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatase activator activity of MTMR9 [cytosol]", "activity_id": "9947649", "ec_numbers": null, "accessions": [ "Q96QG7" ] } ], "citations": [ { "id": "PMID:12890864", "title": "Characterization of myotubularin-related protein 7 and its binding partner, myotubularin-related protein 9", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12890864/" } ], "x": 7740, "y": 1106.6875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809254", "label": "MTMR8 binds MTMR9", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6809254", "summary": "MTMR8 binds MTMR9 is a Reactome release 97 curated reaction event. The source record connects MTMR8, MTMR9 to MTMR8:MTMR9 in cytosol.", "equation": "MTMR8 + MTMR9 → MTMR8:MTMR9", "inputs": [ { "node_id": "R-HSA-1604671", "reactome_id": "R-HSA-1604671", "label": "MTMR8", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6809239", "reactome_id": "R-HSA-6809239", "label": "MTMR9", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6809252", "reactome_id": "R-HSA-6809252", "label": "MTMR8:MTMR9", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatase activator activity of MTMR9 [cytosol]", "activity_id": "9960138", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:22647598", "title": "Myotubularin-related protein (MTMR) 9 determines the enzymatic activity, substrate specificity, and role in autophagy of MTMR8", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22647598/" } ], "x": 2381.3333333333335, "y": 1882.0342465753424, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809263", "label": "EHHADH hydrates trans-2,3-dehydrohexacosanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:39213" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809263", "summary": "EHHADH hydrates trans-2,3-dehydrohexacosanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects trans-2,3-dehydrohexacosanoyl-CoA, H2O to 3-hydroxyhexacosanoyl-CoA in peroxisomal matrix and cross-references RHEA:39213.", "equation": "trans-2,3-dehydrohexacosanoyl-CoA + H2O → 3-hydroxyhexacosanoyl-CoA", "inputs": [ { "node_id": "R-ALL-390249", "reactome_id": "R-ALL-390249", "label": "trans-2,3-dehydrohexacosanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:74281", "formula": "C47H80N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-390248", "reactome_id": "R-ALL-390248", "label": "3-hydroxyhexacosanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76378", "formula": "C47H82N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-hydroxyacyl-CoA dehydratase activity of EHHADH [peroxisomal matrix]", "activity_id": "6809277", "ec_numbers": [ "4.2.1.150" ], "accessions": [ "Q08426" ] } ], "citations": [ { "id": "PMID:1651711", "title": "Import of human bifunctional enzyme into peroxisomes of human hepatoma cells in vitro", "journal": "Biochem. 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The source record connects 3-hydroxyhexacosanoyl-CoA, NAD+ to 3-ketohexacosanoyl-CoA, NADH, H+ in peroxisomal matrix and cross-references RHEA:78637.", "equation": "3-hydroxyhexacosanoyl-CoA + NAD+ → 3-ketohexacosanoyl-CoA + NADH + H+", "inputs": [ { "node_id": "R-ALL-390248", "reactome_id": "R-ALL-390248", "label": "3-hydroxyhexacosanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:76378", "formula": "C47H82N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192307", "reactome_id": "R-ALL-192307", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-390241", "reactome_id": "R-ALL-390241", "label": "3-ketohexacosanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73980", "formula": "C47H80N7O18P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-192305", "reactome_id": "R-ALL-192305", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of EHHADH [peroxisomal matrix]", "activity_id": "6809275", "ec_numbers": [ "1.1.1.35" ], "accessions": [ "Q08426" ] } ], "citations": [ { "id": "PMID:1651711", "title": "Import of human bifunctional enzyme into peroxisomes of human hepatoma cells in vitro", "journal": "Biochem. 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The source record connects NADH, H2O to NMNH, H+, AMP in peroxisomal matrix and cross-references RHEA:48869.", "equation": "NADH + H2O → NMNH + 2 H+ + AMP", "inputs": [ { "node_id": "R-ALL-192305", "reactome_id": "R-ALL-192305", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6809289", "reactome_id": "R-ALL-6809289", "label": "NMNH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90832", "formula": "C11H15N2O8P", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389620", "reactome_id": "R-ALL-389620", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NADH pyrophosphatase activity of NUDT12 [peroxisomal matrix]", "activity_id": "6809293", "ec_numbers": [ "3.6.1.22" ], "accessions": [ "Q9BQG2" ] } ], "citations": [ { "id": "PMID:12790796", "title": "Mammalian NADH diphosphatases of the Nudix family: cloning and characterization of the human peroxisomal NUDT12 protein", "journal": "Biochem. J.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12790796/" } ], "x": 5948.666666666667, "y": 2828.592105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809309", "label": "MTMR6 binds MTMR9", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6809309", "summary": "MTMR6 binds MTMR9 is a Reactome release 97 curated reaction event. The source record connects MTMR6, MTMR9 to MTMR6:MTMR9 in cytosol.", "equation": "MTMR6 + MTMR9 → MTMR6:MTMR9", "inputs": [ { "node_id": "R-HSA-1604666", "reactome_id": "R-HSA-1604666", "label": "MTMR6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6809239", "reactome_id": "R-HSA-6809239", "label": "MTMR9", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6809246", "reactome_id": "R-HSA-6809246", "label": "MTMR6:MTMR9", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatase activator activity of MTMR9 [cytosol]", "activity_id": "9960138", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:19038970", "title": "MTMR9 increases MTMR6 enzyme activity, stability, and role in apoptosis", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19038970/" } ], "x": 2660, "y": 1882.0342465753424, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809320", "label": "PI(3,5)P2 is dephosphorylated to PI5P by MTMR9-bound MTMR6 or MTMR8 at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:39019" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809320", "summary": "PI(3,5)P2 is dephosphorylated to PI5P by MTMR9-bound MTMR6 or MTMR8 at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(3,5)P2, H2O to PI5P, Pi in plasma membrane · cytosol and cross-references RHEA:39019.", "equation": "PI(3,5)P2 + H2O → PI5P + Pi", "inputs": [ { "node_id": "R-ALL-1806224", "reactome_id": "R-ALL-1806224", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1806240", "reactome_id": "R-ALL-1806240", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity of MTMR6:MTMR9,MTMR8:MTMR9 [cytosol]", "activity_id": "6809317", "ec_numbers": [ "3.1.3.95" ], "accessions": [] } ], "citations": [ { "id": "PMID:22647598", "title": "Myotubularin-related protein (MTMR) 9 determines the enzymatic activity, substrate specificity, and role in autophagy of MTMR8", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22647598/" }, { "id": "PMID:19038970", "title": "MTMR9 increases MTMR6 enzyme activity, stability, and role in apoptosis", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19038970/" } ], "x": 2381.3333333333335, "y": 1642.2397260273972, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809325", "label": "PI3P is dephosphorylated to PI by MTMR9-bound MTMR8 or MTMR6 at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:12316" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809325", "summary": "PI3P is dephosphorylated to PI by MTMR9-bound MTMR8 or MTMR6 at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI3P, H2O to PI, Pi in plasma membrane · cytosol and cross-references RHEA:12316.", "equation": "PI3P + H2O → PI + Pi", "inputs": [ { "node_id": "R-ALL-1806276", "reactome_id": "R-ALL-1806276", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426658", "reactome_id": "R-ALL-426658", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate phosphatase activity of MTMR6:MTMR9,MTMR8:MTMR9 [cytosol]", "activity_id": "6809326", "ec_numbers": [ "3.1.3.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:22647598", "title": "Myotubularin-related protein (MTMR) 9 determines the enzymatic activity, substrate specificity, and role in autophagy of MTMR8", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22647598/" } ], "x": 2660, "y": 1642.2397260273972, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809354", "label": "NUDT7 hydrolyses CoA-SH to 3',5'-ADP and PPANT", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:64989" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809354", "summary": "NUDT7 hydrolyses CoA-SH to 3',5'-ADP and PPANT is a Reactome release 97 curated reaction event. The source record connects CoA-SH, H2O to PPANT, PAP, H+ in peroxisomal matrix and cross-references RHEA:64989.", "equation": "CoA-SH + H2O → PPANT + PAP + 2 H+", "inputs": [ { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6809358", "reactome_id": "R-ALL-6809358", "label": "PPANT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61723", "formula": "C11H21N2O7PS", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9641154", "reactome_id": "R-ALL-9641154", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "coenzyme A diphosphatase activity of NUDT7 [peroxisomal matrix]", "activity_id": "6809361", "ec_numbers": [ "3.6.1.77" ], "accessions": [ "P0C024" ] } ], "citations": [ { "id": "PMID:11415433", "title": "The mouse Nudt7 gene encodes a peroxisomal nudix hydrolase specific for coenzyme A and its derivatives", "journal": "Biochem. J.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11415433/" }, { "id": "PMID:18799520", "title": "The nudix hydrolase 7 is an Acyl-CoA diphosphatase involved in regulating peroxisomal coenzyme A homeostasis", "journal": "J. Biochem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18799520/" } ], "x": 590, "y": 1558.6491228070176, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809561", "label": "I(1,3)P2 is dephosphorylated into I1P by MTMR7", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:57841" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809561", "summary": "I(1,3)P2 is dephosphorylated into I1P by MTMR7 is a Reactome release 97 curated reaction event. The source record connects I(1,3)P2, H2O to I1P, Pi in cytosol and cross-references RHEA:57841.", "equation": "I(1,3)P2 + H2O → I1P + Pi", "inputs": [ { "node_id": "R-ALL-1629769", "reactome_id": "R-ALL-1629769", "label": "I(1,3)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83242", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-870330", "reactome_id": "R-ALL-870330", "label": "I1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58433", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol bisphosphate phosphatase activity of MTMR7 [cytosol]", "activity_id": "6809559", "ec_numbers": [ "3.1.3" ], "accessions": [ "Q9Y216" ] } ], "citations": [ { "id": "PMID:12890864", "title": "Characterization of myotubularin-related protein 7 and its binding partner, myotubularin-related protein 9", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12890864/" } ], "x": 7740, "y": 895.9791666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809565", "label": "I(1,3)P2 is dephosphorylated into I1P by MTMR7:MTMR9", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "inositol-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:57841" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809565", "summary": "I(1,3)P2 is dephosphorylated into I1P by MTMR7:MTMR9 is a Reactome release 97 curated reaction event. The source record connects I(1,3)P2, H2O to Pi, I1P in cytosol and cross-references RHEA:57841.", "equation": "I(1,3)P2 + H2O → Pi + I1P", "inputs": [ { "node_id": "R-ALL-1629769", "reactome_id": "R-ALL-1629769", "label": "I(1,3)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83242", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-870330", "reactome_id": "R-ALL-870330", "label": "I1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58433", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "inositol bisphosphate phosphatase activity of MTMR7:MTMR9 [cytosol]", "activity_id": "6809566", "ec_numbers": [ "3.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:12890864", "title": "Characterization of myotubularin-related protein 7 and its binding partner, myotubularin-related protein 9", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12890864/" } ], "x": 8018.666666666667, "y": 895.9791666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809680", "label": "MTMR12 binds MTM1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6809680", "summary": "MTMR12 binds MTM1 is a Reactome release 97 curated reaction event. The source record connects MTM1, MTMR12 to MTM1:MTMR12 in cytosol.", "equation": "MTM1 + MTMR12 → MTM1:MTMR12", "inputs": [ { "node_id": "R-HSA-1604646", "reactome_id": "R-HSA-1604646", "label": "MTM1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6809657", "reactome_id": "R-HSA-6809657", "label": "MTMR12", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6809679", "reactome_id": "R-HSA-6809679", "label": "MTM1:MTMR12", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatase activator activity of MTMR12 [cytosol]", "activity_id": "9959984", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12847286", "title": "Identification of myotubularin as the lipid phosphatase catalytic subunit associated with the 3-phosphatase adapter protein, 3-PAP", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12847286/" }, { "id": "PMID:23818870", "title": "Loss of catalytically inactive lipid phosphatase myotubularin-related protein 12 impairs myotubularin stability and promotes centronuclear myopathy in zebrafish", "journal": "PLoS Genet.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23818870/" }, { "id": "PMID:1655747", "title": "Isolation and characterization of two 3-phosphatases that hydrolyze both phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate", "journal": "J. Biol. Chem.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1655747/" } ], "x": 2938.666666666667, "y": 1882.0342465753424, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809707", "label": "MTMR12 binds MTMR2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6809707", "summary": "MTMR12 binds MTMR2 is a Reactome release 97 curated reaction event. The source record connects MTMR2, MTMR12 to MTMR2:MTMR12 in cytosol.", "equation": "MTMR2 + MTMR12 → MTMR2:MTMR12", "inputs": [ { "node_id": "R-HSA-1604662", "reactome_id": "R-HSA-1604662", "label": "MTMR2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6809657", "reactome_id": "R-HSA-6809657", "label": "MTMR12", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6809705", "reactome_id": "R-HSA-6809705", "label": "MTMR2:MTMR12", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12847286", "title": "Identification of myotubularin as the lipid phosphatase catalytic subunit associated with the 3-phosphatase adapter protein, 3-PAP", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12847286/" } ], "x": 2381.3333333333335, "y": 1898.0205479452056, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809720", "label": "PI3P is dephosphorylated to PI by MTM1:MTMR12", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane · cytosol", "compartment_ids": [ "GO:0031901", "GO:0005829" ], "rhea_ids": [ "RHEA:12316" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809720", "summary": "PI3P is dephosphorylated to PI by MTM1:MTMR12 is a Reactome release 97 curated reaction event. The source record connects H2O, PI3P to PI, Pi in early endosome membrane · cytosol and cross-references RHEA:12316.", "equation": "H2O + PI3P → PI + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1806200", "reactome_id": "R-ALL-1806200", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1806199", "reactome_id": "R-ALL-1806199", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate phosphatase activity of MTM1:MTMR12 [cytosol]", "activity_id": "6809714", "ec_numbers": [ "3.1.3.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:1655747", "title": "Isolation and characterization of two 3-phosphatases that hydrolyze both phosphatidylinositol 3-phosphate and inositol 1,3-bisphosphate", "journal": "J. Biol. Chem.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1655747/" }, { "id": "PMID:12847286", "title": "Identification of myotubularin as the lipid phosphatase catalytic subunit associated with the 3-phosphatase adapter protein, 3-PAP", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12847286/" }, { "id": "PMID:23818870", "title": "Loss of catalytically inactive lipid phosphatase myotubularin-related protein 12 impairs myotubularin stability and promotes centronuclear myopathy in zebrafish", "journal": "PLoS Genet.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23818870/" } ], "x": 2938.666666666667, "y": 1642.2397260273972, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809764", "label": "SBF1 binds MTMR2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6809764", "summary": "SBF1 binds MTMR2 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects MTMR2, SBF1 to MTMR2:SBF1 in cytosol · endoplasmic reticulum membrane.", "equation": "MTMR2 + SBF1 → MTMR2:SBF1", "inputs": [ { "node_id": "R-HSA-1604662", "reactome_id": "R-HSA-1604662", "label": "MTMR2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6809747", "reactome_id": "R-HSA-6809747", "label": "SBF1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6809761", "reactome_id": "R-HSA-6809761", "label": "MTMR2:SBF1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein-membrane adaptor activity of SBF1 [endoplasmic reticulum membrane]", "activity_id": "9949585", "ec_numbers": null, "accessions": [ "O95248" ] } ], "citations": [ { "id": "PMID:14530412", "title": "Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14530412/" }, { "id": "PMID:12668758", "title": "Regulation of myotubularin-related (MTMR)2 phosphatidylinositol phosphatase by MTMR5, a catalytically inactive phosphatase", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12668758/" }, { "id": "PMID:25522275", "title": "SBF-1 exerts strong anticervical cancer effect through inducing endoplasmic reticulum stress-associated cell death via targeting sarco/endoplasmic reticulum Ca(2+)-ATPase 2", "journal": "Cell Death Dis", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25522275/" } ], "x": 2381.3333333333335, "y": 1887.36301369863, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-6809777", "label": "PI3P is dephosphorylated to PI by MTMR2:SBF1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6809777", "summary": "PI3P is dephosphorylated to PI by MTMR2:SBF1 is a Reactome release 97 curated reaction event. The source record connects PI3P, H2O to Pi, PI in cytosol · endoplasmic reticulum membrane.", "equation": "PI3P + H2O → Pi + PI", "inputs": [ { "node_id": "R-ALL-5676030", "reactome_id": "R-ALL-5676030", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:26034", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate phosphatase activity of MTMR2:SBF1 [endoplasmic reticulum membrane]", "activity_id": "6809771", "ec_numbers": [ "3.1.3.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:12668758", "title": "Regulation of myotubularin-related (MTMR)2 phosphatidylinositol phosphatase by MTMR5, a catalytically inactive phosphatase", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12668758/" }, { "id": "PMID:14530412", "title": "Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14530412/" }, { "id": "PMID:25522275", "title": "SBF-1 exerts strong anticervical cancer effect through inducing endoplasmic reticulum stress-associated cell death via targeting sarco/endoplasmic reticulum Ca(2+)-ATPase 2", "journal": "Cell Death Dis", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25522275/" } ], "x": 2381.3333333333335, "y": 1823.4178082191781, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809778", "label": "PI(3,5)P2 is dephosphorylated to PI5P by MTMR2:SBF1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:39019" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809778", "summary": "PI(3,5)P2 is dephosphorylated to PI5P by MTMR2:SBF1 is a Reactome release 97 curated reaction event. The source record connects PI(3,5)P2, H2O to PI5P, Pi in cytosol · endoplasmic reticulum membrane and cross-references RHEA:39019.", "equation": "PI(3,5)P2 + H2O → PI5P + Pi", "inputs": [ { "node_id": "R-ALL-6809774", "reactome_id": "R-ALL-6809774", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-422529", "reactome_id": "R-ALL-422529", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity of MTMR2:SBF1 [endoplasmic reticulum membrane]", "activity_id": "6809772", "ec_numbers": [ "3.1.3.95" ], "accessions": [] } ], "citations": [ { "id": "PMID:12668758", "title": "Regulation of myotubularin-related (MTMR)2 phosphatidylinositol phosphatase by MTMR5, a catalytically inactive phosphatase", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12668758/" }, { "id": "PMID:14530412", "title": "Membrane association of myotubularin-related protein 2 is mediated by a pleckstrin homology-GRAM domain and a coiled-coil dimerization module", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14530412/" }, { "id": "PMID:25522275", "title": "SBF-1 exerts strong anticervical cancer effect through inducing endoplasmic reticulum stress-associated cell death via targeting sarco/endoplasmic reticulum Ca(2+)-ATPase 2", "journal": "Cell Death Dis", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25522275/" } ], "x": 2381.3333333333335, "y": 1647.5684931506848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809785", "label": "MTMR2 dimerizes", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6809785", "summary": "MTMR2 dimerizes is a Reactome release 97 curated reaction event. The source record connects MTMR2 to MTMR2 homodimer in cytosol.", "equation": "2 MTMR2 → MTMR2 homodimer", "inputs": [ { "node_id": "R-HSA-1604662", "reactome_id": "R-HSA-1604662", "label": "MTMR2", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6809787", "reactome_id": "R-HSA-6809787", "label": "MTMR2 homodimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16399794", "title": "Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2", "journal": "Hum. Mol. Genet.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16399794/" } ], "x": 2660, "y": 1898.0205479452056, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809793", "label": "MTMR2 binds SBF2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6809793", "summary": "MTMR2 binds SBF2 is a Reactome release 97 curated reaction event. The source record connects MTMR2 homodimer, SBF2 homodimer to MTMR2:SBF2 Tetramer in cytosol.", "equation": "MTMR2 homodimer + SBF2 homodimer → MTMR2:SBF2 Tetramer", "inputs": [ { "node_id": "R-HSA-6809787", "reactome_id": "R-HSA-6809787", "label": "MTMR2 homodimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-6809794", "reactome_id": "R-HSA-6809794", "label": "SBF2 homodimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6809791", "reactome_id": "R-HSA-6809791", "label": "MTMR2:SBF2 Tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatase activator activity of SBF2 homodimer [cytosol]", "activity_id": "9960143", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:16399794", "title": "Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2", "journal": "Hum. Mol. Genet.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16399794/" } ], "x": 2660, "y": 1887.36301369863, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809808", "label": "ECI2 isomerizes 3Z-enoyl-CoA to 2E-enoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:46045" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809808", "summary": "ECI2 isomerizes 3Z-enoyl-CoA to 2E-enoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3Z-octenoyl-CoA to 2E-octenoyl-CoA in peroxisomal matrix and cross-references RHEA:46045.", "equation": "3Z-octenoyl-CoA → 2E-octenoyl-CoA", "inputs": [ { "node_id": "R-ALL-6809801", "reactome_id": "R-ALL-6809801", "label": "3Z-octenoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:85640", "formula": "C29H44N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6809800", "reactome_id": "R-ALL-6809800", "label": "2E-octenoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62242", "formula": "C29H44N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "delta(3)-delta(2)-enoyl-CoA isomerase activity of ECI2 trimer [peroxisomal matrix]", "activity_id": "6809807", "ec_numbers": [ "5.3.3.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:10419495", "title": "Characterization of PECI, a novel monofunctional Delta(3), Delta(2)-enoyl-CoA isomerase of mammalian peroxisomes", "journal": "J. Biol. Chem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10419495/" }, { "id": "PMID:25515061", "title": "Human Δ³,Δ²-enoyl-CoA isomerase, type 2: a structural enzymology study on the catalytic role of its ACBP domain and helix-10", "journal": "FEBS J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25515061/" } ], "x": 590, "y": 1634.0877192982457, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809810", "label": "PECR reduces 2E-phytenoyl-CoA to phytanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal membrane · peroxisomal matrix", "compartment_ids": [ "GO:0005778", "GO:0005782" ], "rhea_ids": [ "RHEA:33764" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809810", "summary": "PECR reduces 2E-phytenoyl-CoA to phytanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects 2E-phytenoyl-CoA, H+, NADPH to NADP+, Phytanoyl-CoA in peroxisomal membrane · peroxisomal matrix and cross-references RHEA:33764.", "equation": "2E-phytenoyl-CoA + H+ + NADPH → NADP+ + Phytanoyl-CoA", "inputs": [ { "node_id": "R-ALL-6809811", "reactome_id": "R-ALL-6809811", "label": "2E-phytenoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "OtherEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113602", "reactome_id": "R-ALL-113602", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-389556", "reactome_id": "R-ALL-389556", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389615", "reactome_id": "R-ALL-389615", "label": "Phytanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57391", "formula": "C41H70N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "trans-2-enoyl-CoA reductase (NADPH) activity of PECR tetramer [peroxisomal membrane]", "activity_id": "6809574", "ec_numbers": [ "1.3.1.38" ], "accessions": [] } ], "citations": [ { "id": "PMID:10811639", "title": "Molecular cloning and expression of mammalian peroxisomal trans-2-enoyl-coenzyme A reductase cDNAs", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10811639/" }, { "id": "PMID:16546181", "title": "Peroxisomal trans-2-enoyl-CoA reductase is involved in phytol degradation", "journal": "FEBS Lett.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16546181/" }, { "id": "PMID:20558530", "title": "Biochemistry and genetics of inherited disorders of peroxisomal fatty acid metabolism", "journal": "J. Lipid Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20558530/" } ], "x": 590, "y": 1682.3684210526317, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809944", "label": "PI(3,5)P2 is dephosphorylated to PI5P by the MTMR2:SBF2 tetramer at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:39019" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809944", "summary": "PI(3,5)P2 is dephosphorylated to PI5P by the MTMR2:SBF2 tetramer at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI(3,5)P2, H2O to PI5P, Pi in plasma membrane · cytosol and cross-references RHEA:39019.", "equation": "PI(3,5)P2 + H2O → PI5P + Pi", "inputs": [ { "node_id": "R-ALL-1806224", "reactome_id": "R-ALL-1806224", "label": "PI(3,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57923", "formula": "C11H14O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1806240", "reactome_id": "R-ALL-1806240", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3,5-bisphosphate 3-phosphatase activity of MTMR2:SBF2 Tetramer [cytosol]", "activity_id": "6809934", "ec_numbers": [ "3.1.3.95" ], "accessions": [] } ], "citations": [ { "id": "PMID:16399794", "title": "Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2", "journal": "Hum. Mol. Genet.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16399794/" } ], "x": 2660, "y": 1647.5684931506848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6809975", "label": "PI3P is dephosphorylated to PI by the MTMR2:SBF2 tetramer at the plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:12316" ], "source_url": "https://reactome.org/content/detail/R-HSA-6809975", "summary": "PI3P is dephosphorylated to PI by the MTMR2:SBF2 tetramer at the plasma membrane is a Reactome release 97 curated reaction event. The source record connects PI3P, H2O to PI, Pi in plasma membrane · cytosol and cross-references RHEA:12316.", "equation": "PI3P + H2O → PI + Pi", "inputs": [ { "node_id": "R-ALL-1806276", "reactome_id": "R-ALL-1806276", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-426658", "reactome_id": "R-ALL-426658", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate phosphatase activity of MTMR2:SBF2 Tetramer [cytosol]", "activity_id": "6809931", "ec_numbers": [ "3.1.3.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:16399794", "title": "Multi-level regulation of myotubularin-related protein-2 phosphatase activity by myotubularin-related protein-13/set-binding factor-2", "journal": "Hum. Mol. Genet.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16399794/" } ], "x": 2938.666666666667, "y": 1647.5684931506848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6810030", "label": "MTMR2 binds MTMR10", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6810030", "summary": "MTMR2 binds MTMR10 is a Reactome release 97 curated reaction event. The source record connects MTMR2, MTMR10 to MTMR2:MTMR10 in cytosol.", "equation": "MTMR2 + MTMR10 → MTMR2:MTMR10", "inputs": [ { "node_id": "R-HSA-1604662", "reactome_id": "R-HSA-1604662", "label": "MTMR2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-6810028", "reactome_id": "R-HSA-6810028", "label": "MTMR10", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6810024", "reactome_id": "R-HSA-6810024", "label": "MTMR2:MTMR10", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:26496610", "title": "A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances", "journal": "Cell", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26496610/" } ], "x": 2938.666666666667, "y": 1898.0205479452056, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6810076", "label": "DDO oxidizes D-Asp to OA", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:12513" ], "source_url": "https://reactome.org/content/detail/R-HSA-6810076", "summary": "DDO oxidizes D-Asp to OA is a Reactome release 97 curated reaction event. The source record connects D-Asp, H2O, O2 to OA, NH4+, H2O2 in peroxisomal matrix and cross-references RHEA:12513.", "equation": "D-Asp + H2O + O2 → OA + NH4+ + H2O2", "inputs": [ { "node_id": "R-ALL-6810061", "reactome_id": "R-ALL-6810061", "label": "D-Asp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29990", "formula": "C4H6NO4", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113535", "reactome_id": "R-ALL-113535", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6810070", "reactome_id": "R-ALL-6810070", "label": "OA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389843", "reactome_id": "R-ALL-389843", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113575", "reactome_id": "R-ALL-113575", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "D-aspartate oxidase activity of DDO:FAD [peroxisomal matrix]", "activity_id": "6810051", "ec_numbers": [ "1.4.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9820813", "title": "C-terminal tripeptide Ser-Asn-Leu (SNL) of human D-aspartate oxidase is a functional peroxisome-targeting signal", "journal": "Biochem. J.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9820813/" }, { "id": "PMID:25747990", "title": "Characterization of the enzymatic and structural properties of human D-aspartate oxidase and comparison with those of the rat and mouse enzymes", "journal": "Biol. Pharm. Bull.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25747990/" }, { "id": "PMID:20603179", "title": "Thiolactomycin inhibits D-aspartate oxidase: a novel approach to probing the active site environment", "journal": "Biochimie", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20603179/" }, { "id": "PMID:1601857", "title": "The primary structure of the flavoprotein D-aspartate oxidase from beef kidney", "journal": "J. Biol. Chem.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1601857/" }, { "id": "PMID:9163533", "title": "Structural and functional characterization of the human brain D-aspartate oxidase", "journal": "J. Biochem.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9163533/" } ], "x": 868.6666666666667, "y": 3138.714285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6810392", "label": "TMEM55B translocates to the nucleus", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "late endosome membrane · nuclear envelope", "compartment_ids": [ "GO:0031902", "GO:0005635" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6810392", "summary": "TMEM55B translocates to the nucleus is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects TMEM55B to TMEM55B in late endosome membrane · nuclear envelope.", "equation": "TMEM55B → TMEM55B", "inputs": [ { "node_id": "R-HSA-6810395", "reactome_id": "R-HSA-6810395", "label": "TMEM55B", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "late endosome membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-6810405", "reactome_id": "R-HSA-6810405", "label": "TMEM55B", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:17940011", "title": "Type I phosphatidylinositol-4,5-bisphosphate 4-phosphatase regulates stress-induced apoptosis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17940011/" } ], "x": 2938.666666666667, "y": 1914.0068493150684, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-6810410", "label": "PI(4,5)P2 is dephosphorylated to PI5P by TMEM55B in the nucleus", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:25675" ], "source_url": "https://reactome.org/content/detail/R-HSA-6810410", "summary": "PI(4,5)P2 is dephosphorylated to PI5P by TMEM55B in the nucleus is a Reactome release 97 curated reaction event. The source record connects PI(4,5)P2, H2O to Pi, PI5P in nucleoplasm and cross-references RHEA:25675.", "equation": "PI(4,5)P2 + H2O → Pi + PI5P", "inputs": [ { "node_id": "R-ALL-6810412", "reactome_id": "R-ALL-6810412", "label": "PI(4,5)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113518", "reactome_id": "R-ALL-113518", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113550", "reactome_id": "R-ALL-113550", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-6810408", "reactome_id": "R-ALL-6810408", "label": "PI5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-4,5-bisphosphate 4-phosphatase activity of TMEM55B [nucleoplasm]", "activity_id": "6810409", "ec_numbers": [ "3.1.3.78" ], "accessions": [ "Q86T03" ] } ], "citations": [ { "id": "PMID:17940011", "title": "Type I phosphatidylinositol-4,5-bisphosphate 4-phosphatase regulates stress-induced apoptosis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17940011/" } ], "x": 2381.3333333333335, "y": 1652.8972602739725, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6810472", "label": "NUDT13 hydrolyses AP6A to AP4 and ADP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:51725" ], "source_url": "https://reactome.org/content/detail/R-HSA-6810472", "summary": "NUDT13 hydrolyses AP6A to AP4 and ADP is a Reactome release 97 curated reaction event. The source record connects AP6A, H2O to AP4, ADP, H+ in mitochondrial matrix and cross-references RHEA:51725.", "equation": "AP6A + H2O → AP4 + ADP + 2 H+", "inputs": [ { "node_id": "R-ALL-6810468", "reactome_id": "R-ALL-6810468", "label": "AP6A", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63740", "formula": "C20H24N10O25P6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6810471", "reactome_id": "R-ALL-6810471", "label": "AP4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58450", "formula": "C10H12N5O16P4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyrophosphatase activity of NUDT13 [mitochondrial matrix]", "activity_id": "6810479", "ec_numbers": [ "3.6.1" ], "accessions": [ "Q86X67" ] } ], "citations": [ { "id": "PMID:17824959", "title": "Cloning and characterization of AtNUDT13, a novel mitochondrial Arabidopsis thaliana Nudix hydrolase specific for long-chain diadenosine polyphosphates", "journal": "FEBS J.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17824959/" } ], "x": 3878.666666666667, "y": 3111.0208333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6810474", "label": "NUDT19 hydrolyses acyl-CoA to 3',5'-ADP and acyl-PPANT", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:50045" ], "source_url": "https://reactome.org/content/detail/R-HSA-6810474", "summary": "NUDT19 hydrolyses acyl-CoA to 3',5'-ADP and acyl-PPANT is a Reactome release 97 curated reaction event. The source record connects acyl-CoA, H2O to acyl-PPANT, PAP, H+ in peroxisomal matrix and cross-references RHEA:50045.", "equation": "acyl-CoA + H2O → acyl-PPANT + PAP + 2 H+", "inputs": [ { "node_id": "R-ALL-6810523", "reactome_id": "R-ALL-6810523", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-141343", "reactome_id": "R-ALL-141343", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6810530", "reactome_id": "R-ALL-6810530", "label": "acyl-PPANT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132023", "formula": "C12H20N2O8PSR", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9641154", "reactome_id": "R-ALL-9641154", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "coenzyme A diphosphatase activity of NUDT19 [peroxisomal matrix]", "activity_id": "6810534", "ec_numbers": [ "3.6.1.77" ], "accessions": [ "A8MXV4" ] } ], "citations": [ { "id": "PMID:16185196", "title": "Proteomic analysis of mouse kidney peroxisomes: identification of RP2p as a peroxisomal nudix hydrolase with acyl-CoA diphosphatase activity", "journal": "Biochem. J.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16185196/" } ], "x": 868.6666666666667, "y": 1558.6491228070176, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6810594", "label": "HSD17B14 tetramer oxidises estradiol (EST17b) to estrone (E1)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24617" ], "source_url": "https://reactome.org/content/detail/R-HSA-6810594", "summary": "HSD17B14 tetramer oxidises estradiol (EST17b) to estrone (E1) is a Reactome release 97 curated reaction event. The source record connects NADP+, EST17b to E1, NADPH, H+ in cytosol and cross-references RHEA:24617.", "equation": "NADP+ + EST17b → E1 + NADPH + H+", "inputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193132", "reactome_id": "R-ALL-193132", "label": "EST17b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16469", "formula": "C18H24O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193066", "reactome_id": "R-ALL-193066", "label": "E1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17263", "formula": "C18H22O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "estradiol 17-beta-dehydrogenase [NAD(P)+] activity of HSD17B14 tetramer [cytosol]", "activity_id": "6810590", "ec_numbers": [ "1.1.1.62" ], "accessions": [] } ], "citations": [ { "id": "PMID:17067289", "title": "Structural and biochemical characterization of human orphan DHRS10 reveals a novel cytosolic enzyme with steroid dehydrogenase activity", "journal": "Biochem. J.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17067289/" }, { "id": "PMID:22792371", "title": "17β-Hydroxysteroid dehydrogenase type 14 is a predictive marker for tamoxifen response in oestrogen receptor positive breast cancer", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22792371/" } ], "x": 5008.666666666667, "y": 1612.5681818181818, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6811522", "label": "PI5P is phosphorylated to PI(4,5)P2 by PIP4K2 dimers in the nucleus", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:12281" ], "source_url": "https://reactome.org/content/detail/R-HSA-6811522", "summary": "PI5P is phosphorylated to PI(4,5)P2 by PIP4K2 dimers in the nucleus is a Reactome release 97 curated reaction event. The source record connects PI5P, ATP to ADP, PI(4,5)P2 in nucleoplasm and cross-references RHEA:12281.", "equation": "PI5P + ATP → ADP + PI(4,5)P2", "inputs": [ { "node_id": "R-ALL-6810408", "reactome_id": "R-ALL-6810408", "label": "PI5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57795", "formula": "C11H15O16P2R2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29358", "reactome_id": "R-ALL-29358", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113582", "reactome_id": "R-ALL-113582", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-6810412", "reactome_id": "R-ALL-6810412", "label": "PI(4,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58456", "formula": "C11H14O19P3R2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "1-phosphatidylinositol-5-phosphate 4-kinase activity of PIP4K2 dimers [nucleoplasm]", "activity_id": "6811451", "ec_numbers": [ "2.7.1.149" ], "accessions": [] } ], "citations": [ { "id": "PMID:16949365", "title": "Nuclear PtdIns5P as a transducer of stress signaling: an in vivo role for PIP4Kbeta", "journal": "Mol. Cell", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16949365/" }, { "id": "PMID:10698683", "title": "Nuclear targeting of the beta isoform of type II phosphatidylinositol phosphate kinase (phosphatidylinositol 5-phosphate 4-kinase) by its alpha-helix 7", "journal": "Biochem. J.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10698683/" }, { "id": "PMID:20583997", "title": "PIP4Kbeta interacts with and modulates nuclear localization of the high-activity PtdIns5P-4-kinase isoform PIP4Kalpha", "journal": "Biochem. J.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20583997/" } ], "x": 2660, "y": 1652.8972602739725, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6813740", "label": "GDE1 hydrolyzes GroPIns", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:16502" ], "source_url": "https://reactome.org/content/detail/R-HSA-6813740", "summary": "GDE1 hydrolyzes GroPIns is a Reactome release 97 curated reaction event. 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Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25528375/" } ], "x": 2381.3333333333335, "y": 1828.7465753424658, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-6814797", "label": "ENPP6 hydrolyzes lysophosphatidylcholine", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-6814797", "summary": "ENPP6 hydrolyzes lysophosphatidylcholine is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects lysophosphatidylcholine, H2O to ChoP, MAG in plasma membrane · extracellular region.", "equation": "lysophosphatidylcholine + H2O → ChoP + MAG", "inputs": [ { "node_id": "R-ALL-2239407", "reactome_id": "R-ALL-2239407", "label": "lysophosphatidylcholine", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60479", "formula": "C9H19NO7PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6814794", "reactome_id": "R-ALL-6814794", "label": "ChoP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18132", "formula": "C5H15NO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-6814795", "reactome_id": "R-ALL-6814795", "label": "MAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17408", "formula": "C4H7O4R", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophosphocholine cholinephosphodiesterase activity of ENPP6 [plasma membrane]", "activity_id": "6814792", "ec_numbers": [ "3.1.4.38" ], "accessions": [ "Q6UWR7" ] } ], "citations": [ { "id": "PMID:15788404", "title": "Biochemical and molecular characterization of a novel choline-specific glycerophosphodiester phosphodiesterase belonging to the nucleotide pyrophosphatase/phosphodiesterase family", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15788404/" } ], "x": 2660, "y": 1828.7465753424658, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-70241", "label": "PCK1 phosphorylates OA to yield PEP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10389" ], "source_url": "https://reactome.org/content/detail/R-HSA-70241", "summary": "PCK1 phosphorylates OA to yield PEP is a Reactome release 97 curated reaction event. The source record connects OA, GTP to PEP, CO2, GDP in cytosol and cross-references RHEA:10389.", "equation": "OA + GTP → PEP + CO2 + GDP", "inputs": [ { "node_id": "R-ALL-76213", "reactome_id": "R-ALL-76213", "label": "OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29438", "reactome_id": "R-ALL-29438", "label": "GTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37565", "formula": "C10H12N5O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29492", "reactome_id": "R-ALL-29492", "label": "PEP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58702", "formula": "C3H2O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29420", "reactome_id": "R-ALL-29420", "label": "GDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58189", "formula": "C10H12N5O11P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoenolpyruvate carboxykinase (GTP) activity of PCK1 [cytosol]", "activity_id": "70496", "ec_numbers": [ "4.1.1.32" ], "accessions": [ "P35558" ] } ], "citations": [ { "id": "PMID:11851336", "title": "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site", "journal": "J Mol Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11851336/" } ], "x": 1251.3333333333333, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70262", "label": "PFKFKB dimer dephosphorylates fructose 2,6-bisphosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17290" ], "source_url": "https://reactome.org/content/detail/R-HSA-70262", "summary": "PFKFKB dimer dephosphorylates fructose 2,6-bisphosphate is a Reactome release 97 curated reaction event. The source record connects D-Fructose 2,6-bisphosphate, H2O to Fru(6)P, Pi in cytosol and cross-references RHEA:17290.", "equation": "D-Fructose 2,6-bisphosphate + H2O → Fru(6)P + Pi", "inputs": [ { "node_id": "R-ALL-30533", "reactome_id": "R-ALL-30533", "label": "D-Fructose 2,6-bisphosphate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58579", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fructose-2,6-bisphosphate 2-phosphatase activity of p-PFKFB1 dimer [cytosol]", "activity_id": "177980", "ec_numbers": [ "3.1.3.46" ], "accessions": [] } ], "citations": [ { "id": "PMID:7574501", "title": "6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: a metabolic signaling enzyme.", "journal": "Annu Rev Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7574501/" } ], "x": 311.33333333333337, "y": 384.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70286", "label": "UTP + D-glucose 1-phosphate =\u003e pyrophosphate + UDP-glucose", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "biological-oxidations", "glycogen", "phase-two" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19890" ], "source_url": "https://reactome.org/content/detail/R-HSA-70286", "summary": "UTP + D-glucose 1-phosphate =\u003e pyrophosphate + UDP-glucose is a Reactome release 97 curated reaction event. The source record connects UTP, G1P, H+ to PPi, UDP-Glc in cytosol and cross-references RHEA:19890.", "equation": "UTP + G1P + H+ → PPi + UDP-Glc", "inputs": [ { "node_id": "R-ALL-29494", "reactome_id": "R-ALL-29494", "label": "UTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46398", "formula": "C9H11N2O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29548", "reactome_id": "R-ALL-29548", "label": "G1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58601", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "UTP:glucose-1-phosphate uridylyltransferase activity of 8xUGP2 [cytosol]", "activity_id": "70285", "ec_numbers": [ "2.7.7.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:8631325", "title": "Sequence differences between human muscle and liver cDNAs for UDPglucose pyrophosphorylase and kinetic properties of the recombinant enzymes expressed in Escherichia coli.", "journal": "Eur J Biochem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631325/" }, { "id": "PMID:5427280", "title": "Uridine diphosphate glucose pyrophosphorylase. IV. Crystallization and properties of the enzyme from human liver", "journal": "J Biol Chem", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/5427280/" }, { "id": "PMID:5348606", "title": "Uridine diphosphate glucose pyrophosphorylase. II", "journal": "J Biol Chem", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5348606/" } ], "x": 1251.3333333333333, "y": 756.9615384615385, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70333", "label": "KHK dimer phosphorylates Fru to Fru 1-P", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "fructose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18146" ], "source_url": "https://reactome.org/content/detail/R-HSA-70333", "summary": "KHK dimer phosphorylates Fru to Fru 1-P is a Reactome release 97 curated reaction event. The source record connects ATP, beta-D-Fru to ADP, beta-D-Fru 1-P, H+ in cytosol and cross-references RHEA:18146.", "equation": "ATP + beta-D-Fru → ADP + beta-D-Fru 1-P + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9832977", "reactome_id": "R-ALL-9832977", "label": "beta-D-Fru", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28645", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9832976", "reactome_id": "R-ALL-9832976", "label": "beta-D-Fru 1-P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:138881", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ketohexokinase activity of KHK dimer [cytosol]", "activity_id": "70330", "ec_numbers": [ "2.7.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:12941785", "title": "Properties of normal and mutant recombinant human ketohexokinases and implications for the pathogenesis of essential fructosuria", "journal": "Diabetes", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12941785/" }, { "id": "PMID:7833921", "title": "Molecular basis of essential fructosuria: molecular cloning and mutational analysis of human ketohexokinase (fructokinase)", "journal": "Hum Mol Genet", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7833921/" }, { "id": "PMID:20841500", "title": "Both isoforms of ketohexokinase are dispensable for normal growth and development", "journal": "Physiol. Genomics", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20841500/" }, { "id": "PMID:16266770", "title": "Genes required for fructose metabolism are expressed in Purkinje cells in the cerebellum", "journal": "Brain Res. Mol. Brain Res.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16266770/" }, { "id": "PMID:22371574", "title": "Opposing effects of fructokinase C and A isoforms on fructose-induced metabolic syndrome in mice", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22371574/" }, { "id": "PMID:19237742", "title": "Structures of alternatively spliced isoforms of human ketohexokinase", "journal": "Acta Crystallogr D Biol Crystallogr", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19237742/" } ], "x": 2660, "y": 457.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70342", "label": "ALDOB tetramer cleaves Fru-1-P to GA and DHAP", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "fructose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:30852" ], "source_url": "https://reactome.org/content/detail/R-HSA-70342", "summary": "ALDOB tetramer cleaves Fru-1-P to GA and DHAP is a Reactome release 97 curated reaction event. The source record connects beta-D-Fru 1-P to GA, DHAP in cytosol and cross-references RHEA:30852.", "equation": "beta-D-Fru 1-P → GA + DHAP", "inputs": [ { "node_id": "R-ALL-9832976", "reactome_id": "R-ALL-9832976", "label": "beta-D-Fru 1-P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:138881", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30393", "reactome_id": "R-ALL-30393", "label": "GA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17378", "formula": "C3H6O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-75970", "reactome_id": "R-ALL-75970", "label": "DHAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57642", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fructose-1-phosphate aldolase activity of ALDOB tetramer [cytosol]", "activity_id": "469725", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9610797", "title": "Hereditary fructose intolerance", "journal": "J. Med. Genet.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9610797/" }, { "id": "PMID:11679716", "title": "The structure of human liver fructose-1,6-bisphosphate aldolase", "journal": "Acta Crystallogr D Biol Crystallogr", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11679716/" }, { "id": "PMID:13093749", "title": "[The metabolism of fructose-1-phosphate in the liver.]", "journal": "Biochim Biophys Acta", "year": 1953, "url": "https://pubmed.ncbi.nlm.nih.gov/13093749/" }, { "id": "PMID:5777313", "title": "Distribution of fructose diphosphate aldolase variants in biological systems", "journal": "Biochemistry", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5777313/" }, { "id": "PMID:5353106", "title": "Isolation of fructose diphosphate aldolases A, B, and C", "journal": "Biochemistry", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/5353106/" }, { "id": "PMID:805723", "title": "Kinetic and immunological abnormalities of aldolase B in hereditary fructose intolerance", "journal": "Biochem. Soc. Trans.", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/805723/" } ], "x": 2381.3333333333335, "y": 587.1666666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70349", "label": "DAK dimer phosphorylates D-glyceraldehyde to form D-glyceraldehyde 3-phosphate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "fructose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13942" ], "source_url": "https://reactome.org/content/detail/R-HSA-70349", "summary": "DAK dimer phosphorylates D-glyceraldehyde to form D-glyceraldehyde 3-phosphate is a Reactome release 97 curated reaction event. The source record connects ATP, GA to ADP, GA3P in cytosol and cross-references RHEA:13942.", "equation": "ATP + GA → ADP + GA3P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30393", "reactome_id": "R-ALL-30393", "label": "GA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17378", "formula": "C3H6O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "triokinase activity of TKFC:2Mg2+ dimer [cytosol]", "activity_id": "70346", "ec_numbers": [ "2.7.1.28" ], "accessions": [] } ], "citations": [ { "id": "PMID:4688871", "title": "Dihydroxyacetone metabolism by human erythrocytes: demonstration of triokinase activity and its characterization", "journal": "Blood", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4688871/" }, { "id": "PMID:16289032", "title": "Identification of human and rat FAD-AMP lyase (cyclic FMN forming) as ATP-dependent dihydroxyacetone kinases", "journal": "Biochem. Biophys. Res. Commun.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16289032/" }, { "id": "PMID:13093749", "title": "[The metabolism of fructose-1-phosphate in the liver.]", "journal": "Biochim Biophys Acta", "year": 1953, "url": "https://pubmed.ncbi.nlm.nih.gov/13093749/" }, { "id": "PMID:22098443", "title": "Liver protein profiling in chronic hepatitis C: identification of potential predictive markers for interferon therapy outcome", "journal": "J. Proteome Res.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22098443/" }, { "id": "PMID:24569995", "title": "Bifunctional homodimeric triokinase/FMN cyclase: contribution of protein domains to the activities of the human enzyme and molecular dynamics simulation of domain movements", "journal": "J. Biol. Chem.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24569995/" }, { "id": "PMID:24289155", "title": "Serum dihydroxyacetone kinase peptide m/z 520.3 as predictor of disease severity in patients with compensated chronic hepatitis B", "journal": "J Transl Med", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24289155/" } ], "x": 2938.666666666667, "y": 457.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70355", "label": "GALK1 phosphorylates Gal to Gal1P", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "galactose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13554", "RHEA:13553" ], "source_url": "https://reactome.org/content/detail/R-HSA-70355", "summary": "GALK1 phosphorylates Gal to Gal1P is a Reactome release 97 curated reaction event. The source record connects ATP, alpha-D-Gal to ADP, Gal1P, H+ in cytosol and cross-references RHEA:13554, RHEA:13553.", "equation": "ATP + alpha-D-Gal → ADP + Gal1P + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9931851", "reactome_id": "R-ALL-9931851", "label": "alpha-D-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30170", "reactome_id": "R-ALL-30170", "label": "Gal1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17973", "formula": "C6H13O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "galactokinase activity of GALK1 [cytosol]", "activity_id": "70354", "ec_numbers": [ "2.7.1.6" ], "accessions": [ "P51570" ] } ], "citations": [ { "id": "PMID:7542884", "title": "Comparison of the enzymatic activities of human galactokinase GALK1 and a related human galactokinase protein GK2", "journal": "Biochem Biophys Res Commun", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7542884/" } ], "x": 3321.3333333333335, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70361", "label": "GALT transfers UMP from UDP-Glc to Gal1P to form UDP-Gal", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "galactose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13990" ], "source_url": "https://reactome.org/content/detail/R-HSA-70361", "summary": "GALT transfers UMP from UDP-Glc to Gal1P to form UDP-Gal is a Reactome release 97 curated reaction event. The source record connects UDP-Glc, Gal1P to G1P, UDP-Gal in cytosol and cross-references RHEA:13990.", "equation": "UDP-Glc + Gal1P → G1P + UDP-Gal", "inputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-30170", "reactome_id": "R-ALL-30170", "label": "Gal1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17973", "formula": "C6H13O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29548", "reactome_id": "R-ALL-29548", "label": "G1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58601", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29452", "reactome_id": "R-ALL-29452", "label": "UDP-Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "UDP-glucose:hexose-1-phosphate uridylyltransferase activity of GALT [cytosol]", "activity_id": "70360", "ec_numbers": [ "2.7.7.12" ], "accessions": [ "P07902" ] } ], "citations": [ { "id": "PMID:1897530", "title": "Molecular characterization of two galactosemia mutations: correlation of mutations with highly conserved domains in galactose-1-phosphate uridyl transferase", "journal": "Am J Hum Genet", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1897530/" }, { "id": "PMID:2011574", "title": "Molecular basis of galactosemia: mutations and polymorphisms in the gene encoding human galactose-1-phosphate uridylyltransferase", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2011574/" } ], "x": 3878.666666666667, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70369", "label": "GALE:NAD+ dimer reversibly epimerises UDP-Gal to UDP-Glc", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "galactose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22170" ], "source_url": "https://reactome.org/content/detail/R-HSA-70369", "summary": "GALE:NAD+ dimer reversibly epimerises UDP-Gal to UDP-Glc is a Reactome release 97 curated reaction event. The source record connects UDP-Gal to UDP-Glc in cytosol and cross-references RHEA:22170.", "equation": "UDP-Gal → UDP-Glc", "inputs": [ { "node_id": "R-ALL-29452", "reactome_id": "R-ALL-29452", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29410", "reactome_id": "R-ALL-29410", "label": "UDP-Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58885", "formula": "C15H22N2O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "UDP-glucose 4-epimerase activity of GALE:NAD+ dimer [cytosol]", "activity_id": "70368", "ec_numbers": [ "5.1.3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:15175331", "title": "Determinants of function and substrate specificity in human UDP-galactose 4'-epimerase", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15175331/" }, { "id": "PMID:10801319", "title": "Crystallographic evidence for Tyr 157 functioning as the active site base in human UDP-galactose 4-epimerase", "journal": "Biochemistry", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10801319/" } ], "x": 3321.3333333333335, "y": 554.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70377", "label": "G6PD multimers dehydrogenate G6P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15842" ], "source_url": "https://reactome.org/content/detail/R-HSA-70377", "summary": "G6PD multimers dehydrogenate G6P is a Reactome release 97 curated reaction event. The source record connects G6P, NADP+ to D-Glucono-1,5-lactone 6-phosphate, NADPH, H+ in cytosol and cross-references RHEA:15842.", "equation": "G6P + NADP+ → D-Glucono-1,5-lactone 6-phosphate + NADPH + H+", "inputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31467", "reactome_id": "R-ALL-31467", "label": "D-Glucono-1,5-lactone 6-phosphate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57955", "formula": "C6H9O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucose-6-phosphate dehydrogenase activity of G6PD dimer and tetramer [cytosol]", "activity_id": "70374", "ec_numbers": [ "1.1.1.49" ], "accessions": [] } ], "citations": [ { "id": "PMID:5666113", "title": "Enzymic properties of different types of human erythrocyte glucose-6-phosphate dehydrogenase, with characterization of two new genetic variants", "journal": "J Clin Invest", "year": 1968, "url": "https://pubmed.ncbi.nlm.nih.gov/5666113/" }, { "id": "PMID:10745013", "title": "Human glucose-6-phosphate dehydrogenase: the crystal structure reveals a structural NADP(+) molecule and provides insights into enzyme deficiency", "journal": "Structure", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10745013/" }, { "id": "PMID:24066844", "title": "Glucose-6-phosphate dehydrogenase: a biomarker and potential therapeutic target for cancer", "journal": "Anticancer Agents Med Chem", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24066844/" } ], "x": 868.6666666666667, "y": 992.0714285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70420", "label": "HK1,2,3,GCK,HKDC1 phosphorylate Glc to form G6P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17826" ], "source_url": "https://reactome.org/content/detail/R-HSA-70420", "summary": "HK1,2,3,GCK,HKDC1 phosphorylate Glc to form G6P is a Reactome release 97 curated reaction event. The source record connects ATP, Glc to ADP, G6P, H+ in cytosol and cross-references RHEA:17826.", "equation": "ATP + Glc → ADP + G6P + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70113", "reactome_id": "R-ALL-70113", "label": "Glc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucokinase activity of glucokinase and hexokinases [cytosol]", "activity_id": "70419", "ec_numbers": [ "2.7.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9493266", "title": "The mechanism of regulation of hexokinase: new insights from the crystal structure of recombinant human brain hexokinase complexed with glucose and glucose-6-phosphate", "journal": "Structure", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9493266/" }, { "id": "PMID:25648650", "title": "Coordinated regulatory variation associated with gestational hyperglycaemia regulates expression of the novel hexokinase HKDC1", "journal": "Nat Commun", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25648650/" }, { "id": "PMID:8786021", "title": "Human hexokinase II gene: exon-intron organization, mutation screening in NIDDM, and its relationship to muscle hexokinase activity", "journal": "Diabetologia", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8786021/" }, { "id": "PMID:30517626", "title": "Hepatic HKDC1 Expression Contributes to Liver Metabolism", "journal": "Endocrinology", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30517626/" }, { "id": "PMID:7150652", "title": "Compartmentation of hexokinase in human blood cells. Characterization of soluble and particulate enzymes.", "journal": "Biochim Biophys Acta", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7150652/" }, { "id": "PMID:8325892", "title": "Structure/function studies of human beta-cell glucokinase. Enzymatic properties of a sequence polymorphism, mutations associated with diabetes, and other site-directed mutants.", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8325892/" } ], "x": 311.33333333333337, "y": 530.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70449", "label": "GADPH tetramer dehydrogenates GA3P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10301" ], "source_url": "https://reactome.org/content/detail/R-HSA-70449", "summary": "GADPH tetramer dehydrogenates GA3P is a Reactome release 97 curated reaction event. The source record connects GA3P, Pi, NAD+ to 1,3BPG, NADH, H+ in cytosol and cross-references RHEA:10301.", "equation": "GA3P + Pi + NAD+ → 1,3BPG + NADH + H+", "inputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29800", "reactome_id": "R-ALL-29800", "label": "1,3BPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57604", "formula": "C3H4O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity of GAPDH tetramers [cytosol]", "activity_id": "70448", "ec_numbers": [ "1.2.1.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:2793178", "title": "Members of the human glyceraldehyde-3-phosphate dehydrogenase-related gene family map to dispersed chromosomal locations.", "journal": "Genomics", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2793178/" }, { "id": "PMID:18910716", "title": "Crystalline D-glyceraldehyde-3-phosphate dehydrogenase from rabbit muscle", "journal": "J Biol Chem", "year": 1948, "url": "https://pubmed.ncbi.nlm.nih.gov/18910716/" }, { "id": "PMID:183598", "title": "Isozymes of glyceraldehyde-3-phosphate dehydrogenase in man and other mammals.", "journal": "Ann Hum Genet", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/183598/" }, { "id": "PMID:3170585", "title": "Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3170585/" }, { "id": "PMID:7144574", "title": "Glyceraldehyde-3-phosphate dehydrogenase from human tissues.", "journal": "Methods Enzymol", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/7144574/" }, { "id": "PMID:10714828", "title": "Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells.", "journal": "J Androl", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10714828/" } ], "x": 311.33333333333337, "y": 287.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70454", "label": "TPI1 dimer isomerizes DHAP to GA3P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18587" ], "source_url": "https://reactome.org/content/detail/R-HSA-70454", "summary": "TPI1 dimer isomerizes DHAP to GA3P is a Reactome release 97 curated reaction event. The source record connects DHAP to GA3P in cytosol and cross-references RHEA:18587.", "equation": "DHAP → GA3P", "inputs": [ { "node_id": "R-ALL-75970", "reactome_id": "R-ALL-75970", "label": "DHAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57642", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "triose-phosphate isomerase activity of TPI1 dimer [cytosol]", "activity_id": "70453", "ec_numbers": [ "5.3.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6434534", "title": "Primary structure of human triosephosphate isomerase", "journal": "J Biol Chem", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6434534/" }, { "id": "PMID:16511037", "title": "Structure of a high-resolution crystal form of human triosephosphate isomerase: improvement of crystals using the gel-tube method", "journal": "Acta Crystallogr Sect F Struct Biol Cryst Commun", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16511037/" } ], "x": 868.6666666666667, "y": 433.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70467", "label": "PFK tetramer phosphorylates Fru(6)P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16110" ], "source_url": "https://reactome.org/content/detail/R-HSA-70467", "summary": "PFK tetramer phosphorylates Fru(6)P is a Reactome release 97 curated reaction event. The source record connects Fru(6)P, ATP to F1,6PP, ADP, H+ in cytosol and cross-references RHEA:16110.", "equation": "Fru(6)P + ATP → F1,6PP + ADP + H+", "inputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30014", "reactome_id": "R-ALL-30014", "label": "F1,6PP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32966", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "6-phosphofructokinase activity of PFK tetramer [cytosol]", "activity_id": "70466", "ec_numbers": [ "2.7.1.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:8117307", "title": "Cloning of a complete protein-coding sequence of human platelet-type phosphofructokinase isozyme from pancreatic islet.", "journal": "Biochem Biophys Res Commun", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8117307/" }, { "id": "PMID:7825568", "title": "Functional expression of human mutant phosphofructokinase in yeast: genetic defects in French Canadian and Swiss patients with phosphofructokinase deficiency", "journal": "Am J Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7825568/" }, { "id": "PMID:6451249", "title": "Isozymes of human phosphofructokinase in blood cells and cultured cell lines: molecular and genetic evidence for a trigenic system", "journal": "Blood", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/6451249/" }, { "id": "PMID:6444721", "title": "Isozymes of human phosphofructokinase: identification and subunit structural characterization of a new system", "journal": "Proc Natl Acad Sci U S A", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6444721/" }, { "id": "PMID:2960695", "title": "Characterization of the enzymatic defect in late-onset muscle phosphofructokinase deficiency. New subtype of glycogen storage disease type VII.", "journal": "J Clin Invest", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2960695/" } ], "x": 590, "y": 287.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70471", "label": "GPI dimer isomerizes G6P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11817" ], "source_url": "https://reactome.org/content/detail/R-HSA-70471", "summary": "GPI dimer isomerizes G6P is a Reactome release 97 curated reaction event. The source record connects G6P to Fru(6)P in cytosol and cross-references RHEA:11817.", "equation": "G6P → Fru(6)P", "inputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucose-6-phosphate isomerase activity of GPI dimer [cytosol]", "activity_id": "70470", "ec_numbers": [ "5.3.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:11371164", "title": "The crystal structure of human phosphoglucose isomerase at 1.6 A resolution: implications for catalytic mechanism, cytokine activity and haemolytic anaemia", "journal": "J Mol Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11371164/" }, { "id": "PMID:13538944", "title": "Enzymes of the human erythrocyte. IV. Phosphoglucose isomerase, purification and properties.", "journal": "J Biol Chem", "year": 1958, "url": "https://pubmed.ncbi.nlm.nih.gov/13538944/" }, { "id": "PMID:7989588", "title": "The characterization of gene mutations for human glucose phosphate isomerase deficiency associated with chronic hemolytic anemia", "journal": "J Clin Invest", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7989588/" } ], "x": 311.33333333333337, "y": 481.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70475", "label": "GPI dimer isomerizes Fru(6)P to G6P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11818" ], "source_url": "https://reactome.org/content/detail/R-HSA-70475", "summary": "GPI dimer isomerizes Fru(6)P to G6P is a Reactome release 97 curated reaction event. The source record connects Fru(6)P to G6P in cytosol and cross-references RHEA:11818.", "equation": "Fru(6)P → G6P", "inputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucose-6-phosphate isomerase activity of GPI dimer [cytosol]", "activity_id": "70470", "ec_numbers": [ "5.3.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:7989588", "title": "The characterization of gene mutations for human glucose phosphate isomerase deficiency associated with chronic hemolytic anemia", "journal": "J Clin Invest", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7989588/" }, { "id": "PMID:13538944", "title": "Enzymes of the human erythrocyte. IV. Phosphoglucose isomerase, purification and properties.", "journal": "J Biol Chem", "year": 1958, "url": "https://pubmed.ncbi.nlm.nih.gov/13538944/" } ], "x": 1808.6666666666667, "y": 425.0833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70479", "label": "FBP tetramers hydrolyze F1,6PP to Fru(6)P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11065" ], "source_url": "https://reactome.org/content/detail/R-HSA-70479", "summary": "FBP tetramers hydrolyze F1,6PP to Fru(6)P is a Reactome release 97 curated reaction event. The source record connects H2O, F1,6PP to Fru(6)P, Pi in cytosol and cross-references RHEA:11065.", "equation": "H2O + F1,6PP → Fru(6)P + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30014", "reactome_id": "R-ALL-30014", "label": "F1,6PP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32966", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fructose 1,6-bisphosphate 1-phosphatase activity of FBP tetramer [cytosol]", "activity_id": "70478", "ec_numbers": [ "3.1.3.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:9382095", "title": "Identification of genetic mutations in Japanese patients with fructose-1,6-bisphosphatase deficiency", "journal": "Am J Hum Genet", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9382095/" }, { "id": "PMID:9678974", "title": "Isolation and characterization of an allelic cDNA for human muscle fructose-1,6-bisphosphatase", "journal": "Gene", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9678974/" } ], "x": 1530, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70481", "label": "TPI1 dimer isomerizes GA3P to DHAP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18586" ], "source_url": "https://reactome.org/content/detail/R-HSA-70481", "summary": "TPI1 dimer isomerizes GA3P to DHAP is a Reactome release 97 curated reaction event. The source record connects GA3P to DHAP in cytosol and cross-references RHEA:18586.", "equation": "GA3P → DHAP", "inputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-75970", "reactome_id": "R-ALL-75970", "label": "DHAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57642", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "triose-phosphate isomerase activity of TPI1 dimer [cytosol]", "activity_id": "70453", "ec_numbers": [ "5.3.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:8571957", "title": "Molecular analysis of a series of alleles in humans with reduced activity at the triosephosphate isomerase locus", "journal": "Am J Hum Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8571957/" }, { "id": "PMID:6434534", "title": "Primary structure of human triosephosphate isomerase", "journal": "J Biol Chem", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6434534/" } ], "x": 1251.3333333333333, "y": 489.91666666666663, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70482", "label": "GAPDH tetramers reduce 1,3BPG to GA3P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10302" ], "source_url": "https://reactome.org/content/detail/R-HSA-70482", "summary": "GAPDH tetramers reduce 1,3BPG to GA3P is a Reactome release 97 curated reaction event. The source record connects 1,3BPG, NADH, H+ to GA3P, Pi, NAD+ in cytosol and cross-references RHEA:10302.", "equation": "1,3BPG + NADH + H+ → GA3P + Pi + NAD+", "inputs": [ { "node_id": "R-ALL-29800", "reactome_id": "R-ALL-29800", "label": "1,3BPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57604", "formula": "C3H4O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity of GAPDH tetramers [cytosol]", "activity_id": "70448", "ec_numbers": [ "1.2.1.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:7144574", "title": "Glyceraldehyde-3-phosphate dehydrogenase from human tissues.", "journal": "Methods Enzymol", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/7144574/" }, { "id": "PMID:183598", "title": "Isozymes of glyceraldehyde-3-phosphate dehydrogenase in man and other mammals.", "journal": "Ann Hum Genet", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/183598/" }, { "id": "PMID:10714828", "title": "Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells.", "journal": "J Androl", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10714828/" }, { "id": "PMID:3170585", "title": "Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3170585/" } ], "x": 1530, "y": 295.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70486", "label": "PGK complexes (PGK1,2) phosphorylate 3PG to form 1,3BPG", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14802" ], "source_url": "https://reactome.org/content/detail/R-HSA-70486", "summary": "PGK complexes (PGK1,2) phosphorylate 3PG to form 1,3BPG is a Reactome release 97 curated reaction event. The source record connects ATP, 3PG to ADP, 1,3BPG in cytosol and cross-references RHEA:14802.", "equation": "ATP + 3PG → ADP + 1,3BPG", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29728", "reactome_id": "R-ALL-29728", "label": "3PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58272", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29800", "reactome_id": "R-ALL-29800", "label": "1,3BPG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57604", "formula": "C3H4O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphoglycerate kinase activity of PGK complexes [cytosol]", "activity_id": "70485", "ec_numbers": [ "2.7.2.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:1278465", "title": "Characterization of phosphoglycerate kinase from human spermatozoa", "journal": "Fertil. Steril.", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/1278465/" }, { "id": "PMID:7391027", "title": "Complete amino acid sequence of human phosphoglycerate kinase. Cyanogen bromide peptides and complete amino acid sequence.", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/7391027/" }, { "id": "PMID:6771269", "title": "Complete amino acid sequence of human phosphoglycerate kinase. Isolation and amino acid sequence of tryptic peptides.", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6771269/" }, { "id": "PMID:5009693", "title": "Human phosphoglycerate kinase. I. Crystallization and characterization of normal enzyme", "journal": "J Biol Chem", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/5009693/" } ], "x": 1808.6666666666667, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70494", "label": "Enolase dimers (ENO1,2,3) convert PEP to 2PG", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10166" ], "source_url": "https://reactome.org/content/detail/R-HSA-70494", "summary": "Enolase dimers (ENO1,2,3) convert PEP to 2PG is a Reactome release 97 curated reaction event. The source record connects PEP, H2O to 2PG in cytosol and cross-references RHEA:10166.", "equation": "PEP + H2O → 2PG", "inputs": [ { "node_id": "R-ALL-29492", "reactome_id": "R-ALL-29492", "label": "PEP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58702", "formula": "C3H2O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30485", "reactome_id": "R-ALL-30485", "label": "2PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58289", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphopyruvate hydratase activity of enolase dimer [cytosol]", "activity_id": "71439", "ec_numbers": [ "4.2.1.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:11506403", "title": "Beta-enolase deficiency, a new metabolic myopathy of distal glycolysis", "journal": "Ann Neurol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11506403/" }, { "id": "PMID:3208766", "title": "Complete amino acid sequence of the neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE)", "journal": "Eur J Biochem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3208766/" }, { "id": "PMID:3529090", "title": "Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3529090/" } ], "x": 1251.3333333333333, "y": 425.0833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70501", "label": "PC carboxylates PYR to OA", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis", "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:20845" ], "source_url": "https://reactome.org/content/detail/R-HSA-70501", "summary": "PC carboxylates PYR to OA is a Reactome release 97 curated reaction event. The source record connects ATP, PYR, HCO3- to ADP, Pi, OA in mitochondrial matrix and cross-references RHEA:20845.", "equation": "ATP + PYR + HCO3- → ADP + Pi + OA + H+", "inputs": [ { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29896", "reactome_id": "R-ALL-29896", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113587", "reactome_id": "R-ALL-113587", "label": "OA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyruvate carboxylase activity of PC tetramer [mitochondrial matrix]", "activity_id": "70500", "ec_numbers": [ "6.4.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12437512", "title": "Expression and characterization of a human pyruvate carboxylase variant by retroviral gene transfer", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12437512/" }, { "id": "PMID:10229653", "title": "Structure, function and regulation of pyruvate carboxylase", "journal": "Biochem J", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10229653/" }, { "id": "PMID:18613815", "title": "Structure, mechanism and regulation of pyruvate carboxylase", "journal": "Biochem J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18613815/" }, { "id": "PMID:9585002", "title": "Molecular characterization of pyruvate carboxylase deficiency in two consanguineous families", "journal": "Pediatr Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9585002/" }, { "id": "PMID:36261450", "title": "CryoEM structural exploration of catalytically active enzyme pyruvate carboxylase", "journal": "Nat Commun", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36261450/" }, { "id": "PMID:4826475", "title": "Purification and properties of human liver pyruvate carboxylase", "journal": "Biochem Med", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4826475/" } ], "x": 1251.3333333333333, "y": 295.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70510", "label": "LDH tetramer oxidises LACT to PYR", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23445" ], "source_url": "https://reactome.org/content/detail/R-HSA-70510", "summary": "LDH tetramer oxidises LACT to PYR is a Reactome release 97 curated reaction event. The source record connects LACT, NAD+ to PYR, NADH, H+ in cytosol and cross-references RHEA:23445.", "equation": "LACT + NAD+ → PYR + NADH + H+", "inputs": [ { "node_id": "R-ALL-29708", "reactome_id": "R-ALL-29708", "label": "LACT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16651", "formula": "C3H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-lactate dehydrogenase (NAD+) activity of LDH tetramer [cytosol]", "activity_id": "70509", "ec_numbers": [ "1.1.1.27" ], "accessions": [] } ], "citations": [ { "id": "PMID:18351441", "title": "Identification of a novel human lactate dehydrogenase gene LDHAL6A, which activates transcriptional activities of AP1(PMA)", "journal": "Mol. Biol. Rep.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18351441/" }, { "id": "PMID:1996957", "title": "Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli", "journal": "Biochem. J.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1996957/" }, { "id": "PMID:2440048", "title": "Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2440048/" }, { "id": "PMID:11276087", "title": "Structural basis for altered activity of M- and H-isozyme forms of human lactate dehydrogenase", "journal": "Proteins", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11276087/" }, { "id": "PMID:3435492", "title": "The cDNA and protein sequences of human lactate dehydrogenase B", "journal": "Biochem J", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3435492/" }, { "id": "PMID:11377399", "title": "Selective active site inhibitors of human lactate dehydrogenases A4, B4, and C4", "journal": "Biochem Pharmacol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11377399/" } ], "x": 5008.666666666667, "y": 282.45, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70523", "label": "PXLP-K314-GPT transaminates L-Ala to form PYR", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19454" ], "source_url": "https://reactome.org/content/detail/R-HSA-70523", "summary": "PXLP-K314-GPT transaminates L-Ala to form PYR is a Reactome release 97 curated reaction event. The source record connects L-Ala, 2OG to PYR, L-Glu in cytosol and cross-references RHEA:19454.", "equation": "L-Ala + 2OG → PYR + L-Glu", "inputs": [ { "node_id": "R-ALL-29432", "reactome_id": "R-ALL-29432", "label": "L-Ala", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-alanine:2-oxoglutarate transaminase activity of PXLP-K314-GPT dimer [cytosol]", "activity_id": "70522", "ec_numbers": [ "2.6.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:1931970", "title": "Complete amino acid sequence of human liver cytosolic alanine aminotransferase (GPT) determined by a combination of conventional and mass spectral methods", "journal": "Biochemistry", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1931970/" }, { "id": "PMID:9119391", "title": "Human glutamate pyruvate transaminase (GPT): localization to 8q24.3, cDNA and genomic sequences, and polymorphic sites.", "journal": "Genomics", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9119391/" }, { "id": "PMID:11863375", "title": "cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase", "journal": "Genomics", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11863375/" } ], "x": 4730, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70542", "label": "glutamate + acetyl CoA =\u003e N-acetyl glutamate + CoA", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24293" ], "source_url": "https://reactome.org/content/detail/R-HSA-70542", "summary": "glutamate + acetyl CoA =\u003e N-acetyl glutamate + CoA is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, L-Glu to CoA-SH, NAcGlu, H+ in mitochondrial matrix and cross-references RHEA:24293.", "equation": "Ac-CoA + L-Glu → CoA-SH + NAcGlu + H+", "inputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30471", "reactome_id": "R-ALL-30471", "label": "NAcGlu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:44337", "formula": "C7H9NO5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-glutamate N-acetyltransferase activity, acting on acetyl-CoA as donor of NAGS(19-534) dimer [mitochondrial matrix]", "activity_id": "70539", "ec_numbers": [ "2.3.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12459178", "title": "Cloning and expression of the human N-acetylglutamate synthase gene.", "journal": "Biochem Biophys Res Commun", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12459178/" }, { "id": "PMID:15050968", "title": "Mammalian N-acetylglutamate synthase", "journal": "Mol Genet Metab", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15050968/" } ], "x": 1808.6666666666667, "y": 3141.444444444445, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70555", "label": "2 ATP + NH4+ + HCO3- =\u003e 2 ADP + orthophosphate + carbamoyl phosphate [mitochondrial]", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18030" ], "source_url": "https://reactome.org/content/detail/R-HSA-70555", "summary": "2 ATP + NH4+ + HCO3- =\u003e 2 ADP + orthophosphate + carbamoyl phosphate [mitochondrial] is a Reactome release 97 curated reaction event. The source record connects HCO3-, NH4+, ATP to CAP, Pi, ADP in mitochondrial matrix and cross-references RHEA:18030.", "equation": "HCO3- + NH4+ + 2 ATP → CAP + Pi + 2 ADP + 2 H+", "inputs": [ { "node_id": "R-ALL-29896", "reactome_id": "R-ALL-29896", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113561", "reactome_id": "R-ALL-113561", "label": "NH4+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29676", "reactome_id": "R-ALL-29676", "label": "CAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58228", "formula": "CH2NO5P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbamoyl-phosphate synthase (ammonia) activity of CPS1 [mitochondrial matrix]", "activity_id": "70552", "ec_numbers": [ "6.3.4.16" ], "accessions": [ "P31327" ] } ], "citations": [ { "id": "PMID:6249820", "title": "Human carbamylphosphate synthetase I. Stabilization, purification, and partial characterization of the enzyme from human liver.", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6249820/" }, { "id": "PMID:7028477", "title": "Human carbamoylphosphate synthetase I", "journal": "Enzyme", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/7028477/" }, { "id": "PMID:19754428", "title": "Structural insight on the control of urea synthesis: identification of the binding site for N-acetyl-L-glutamate, the essential allosteric activator of mitochondrial carbamoyl phosphate synthetase", "journal": "Biochem J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19754428/" }, { "id": "PMID:23649895", "title": "Molecular characterization of carbamoyl-phosphate synthetase (CPS1) deficiency using human recombinant CPS1 as a key tool", "journal": "Hum Mutat", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23649895/" }, { "id": "PMID:20800523", "title": "Genetic, structural and biochemical basis of carbamoyl phosphate synthetase 1 deficiency", "journal": "Mol Genet Metab", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20800523/" }, { "id": "PMID:33317798", "title": "CPS1: Looking at an ancient enzyme in a modern light", "journal": "Mol Genet Metab", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33317798/" } ], "x": 1251.3333333333333, "y": 3141.444444444445, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70560", "label": "carbamoyl phosphate + ornithine =\u003e citrulline + orthophosphate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:19514" ], "source_url": "https://reactome.org/content/detail/R-HSA-70560", "summary": "carbamoyl phosphate + ornithine =\u003e citrulline + orthophosphate is a Reactome release 97 curated reaction event. The source record connects CAP, L-Orn to L-Cit, Pi, H+ in mitochondrial matrix and cross-references RHEA:19514.", "equation": "CAP + L-Orn → L-Cit + Pi + H+", "inputs": [ { "node_id": "R-ALL-29676", "reactome_id": "R-ALL-29676", "label": "CAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58228", "formula": "CH2NO5P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113585", "reactome_id": "R-ALL-113585", "label": "L-Orn", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46911", "formula": "C5H13N2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113567", "reactome_id": "R-ALL-113567", "label": "L-Cit", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57743", "formula": "C6H13N3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ornithine carbamoyltransferase activity of OTC trimer [mitochondrial matrix]", "activity_id": "70559", "ec_numbers": [ "2.1.3.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:6372096", "title": "Structure and expression of a complementary DNA for the nuclear coded precursor of human mitochondrial ornithine transcarbamylase.", "journal": "Science", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6372096/" }, { "id": "PMID:11237854", "title": "Human ornithine transcarbamylase: crystallographic insights into substrate recognition and conformational changes", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11237854/" } ], "x": 1251.3333333333333, "y": 3179.666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70569", "label": "arginine + H2O =\u003e ornithine + urea [ARG1]", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20570" ], "source_url": "https://reactome.org/content/detail/R-HSA-70569", "summary": "arginine + H2O =\u003e ornithine + urea [ARG1] is a Reactome release 97 curated reaction event. The source record connects L-Arg, H2O to L-Orn, Urea in cytosol and cross-references RHEA:20570.", "equation": "L-Arg + H2O → L-Orn + Urea", "inputs": [ { "node_id": "R-ALL-29468", "reactome_id": "R-ALL-29468", "label": "L-Arg", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32682", "formula": "C6H15N4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29498", "reactome_id": "R-ALL-29498", "label": "L-Orn", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:46911", "formula": "C5H13N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29514", "reactome_id": "R-ALL-29514", "label": "Urea", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16199", "formula": "CH4N2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arginase activity of ARG1 trimer [cytosol]", "activity_id": "70568", "ec_numbers": [ "3.5.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16141327", "title": "Crystal structure of human arginase I at 1.29-A resolution and exploration of inhibition in the immune response", "journal": "Proc Natl Acad Sci U S A", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16141327/" }, { "id": "PMID:7649538", "title": "Molecular basis of phenotypic variation in patients with argininemia", "journal": "Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7649538/" } ], "x": 1808.6666666666667, "y": 3179.666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70573", "label": "argininosuccinate fumarate + arginine", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24021" ], "source_url": "https://reactome.org/content/detail/R-HSA-70573", "summary": "argininosuccinate fumarate + arginine is a Reactome release 97 curated reaction event. The source record connects ARSUA to L-Arg, FUMA in cytosol and cross-references RHEA:24021.", "equation": "ARSUA → L-Arg + FUMA", "inputs": [ { "node_id": "R-ALL-34575", "reactome_id": "R-ALL-34575", "label": "ARSUA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57472", "formula": "C10H17N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29468", "reactome_id": "R-ALL-29468", "label": "L-Arg", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32682", "formula": "C6H15N4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29586", "reactome_id": "R-ALL-29586", "label": "FUMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29806", "formula": "C4H2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "argininosuccinate lyase activity of ASL tetramer [cytosol]", "activity_id": "70572", "ec_numbers": [ "4.3.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9256435", "title": "Human argininosuccinate lyase: a structural basis for intragenic complementation", "journal": "Proc Natl Acad Sci U S A", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9256435/" }, { "id": "PMID:2263616", "title": "Molecular analysis of human argininosuccinate lyase: mutant characterization and alternative splicing of the coding region", "journal": "Proc Natl Acad Sci U S A", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2263616/" } ], "x": 1251.3333333333333, "y": 3217.888888888889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70577", "label": "ASS1 tetramer:NMRAL1 dimer:NADPH transforms L-Asp and L-Cit to ARSUA", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10933" ], "source_url": "https://reactome.org/content/detail/R-HSA-70577", "summary": "ASS1 tetramer:NMRAL1 dimer:NADPH transforms L-Asp and L-Cit to ARSUA is a Reactome release 97 curated reaction event. The source record connects ATP, L-Asp, L-Cit to ARSUA, AMP, PPi in cytosol and cross-references RHEA:10933.", "equation": "ATP + L-Asp + L-Cit → ARSUA + AMP + PPi + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29448", "reactome_id": "R-ALL-29448", "label": "L-Asp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29968", "reactome_id": "R-ALL-29968", "label": "L-Cit", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57743", "formula": "C6H13N3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-34575", "reactome_id": "R-ALL-34575", "label": "ARSUA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57472", "formula": "C10H17N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "argininosuccinate synthase activity of ASS1 tetramer:NMRAL1 dimer:NADPH [cytosol]", "activity_id": "70576", "ec_numbers": [ "6.3.4.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:19006241", "title": "Mutations and polymorphisms in the human argininosuccinate synthetase (ASS1) gene", "journal": "Hum Mutat", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19006241/" }, { "id": "PMID:18323623", "title": "Structure of human argininosuccinate synthetase", "journal": "Acta Crystallogr D Biol Crystallogr", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18323623/" }, { "id": "PMID:518841", "title": "Isolation and characterization of argininosuccinate synthetase from human liver.", "journal": "Biochemistry", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/518841/" }, { "id": "PMID:17496144", "title": "Restructuring of the dinucleotide-binding fold in an NADP(H) sensor protein", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17496144/" }, { "id": "PMID:18263583", "title": "An NADPH sensor protein (HSCARG) down-regulates nitric oxide synthesis by association with argininosuccinate synthetase and is essential for epithelial cell viability", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18263583/" } ], "x": 1530, "y": 3141.444444444445, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70589", "label": "alpha-ketoglutarate + NH4+ + NAD(P)H + H+ glutamate + NAD(P)+ (GLUD1,2)", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15135", "RHEA:11614" ], "source_url": "https://reactome.org/content/detail/R-HSA-70589", "summary": "alpha-ketoglutarate + NH4+ + NAD(P)H + H+ glutamate + NAD(P)+ (GLUD1,2) is a Reactome release 97 curated reaction event. The source record connects 2OG, NH4+, NAD(P)H to L-Glu, NAD(P)+, H2O in mitochondrial matrix and cross-references RHEA:15135, RHEA:11614.", "equation": "2OG + NH4+ + NAD(P)H + H+ → L-Glu + NAD(P)+ + H2O", "inputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113561", "reactome_id": "R-ALL-113561", "label": "NH4+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-517496", "reactome_id": "R-ALL-517496", "label": "NAD(P)H", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-517495", "reactome_id": "R-ALL-517495", "label": "NAD(P)+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-glutamate dehydrogenase [NAD(P)+] activity of GLUD hexamer [mitochondrial matrix]", "activity_id": "70584", "ec_numbers": [ "1.4.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:11903050", "title": "Expression, purification and characterization of human glutamate dehydrogenase (GDH) allosteric regulatory mutations", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11903050/" }, { "id": "PMID:429360", "title": "Partial amino acid sequence of the glutamate dehydrogenase of human liver and a revision of the sequence of the bovine enzyme", "journal": "J Biol Chem", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/429360/" }, { "id": "PMID:12054821", "title": "The structure of apo human glutamate dehydrogenase details subunit communication and allostery", "journal": "J Mol Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12054821/" } ], "x": 8018.666666666667, "y": 1577.85, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70592", "label": "PXLP-K259-GOT1 transaminates 2-OG and L-Asp", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport", "malate-aspartate-shuttle" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21825" ], "source_url": "https://reactome.org/content/detail/R-HSA-70592", "summary": "PXLP-K259-GOT1 transaminates 2-OG and L-Asp is a Reactome release 97 curated reaction event. The source record connects L-Asp, 2OG to OA, L-Glu in cytosol and cross-references RHEA:21825.", "equation": "L-Asp + 2OG → OA + L-Glu", "inputs": [ { "node_id": "R-ALL-29448", "reactome_id": "R-ALL-29448", "label": "L-Asp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76213", "reactome_id": "R-ALL-76213", "label": "OA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-aspartate:2-oxoglutarate transaminase activity of PXLP-K259-GOT1 dimer [cytosol]", "activity_id": "70580", "ec_numbers": [ "2.6.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2241899", "title": "The amino acid sequence of cytosolic aspartate aminotransferase from human liver", "journal": "Biochem J", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2241899/" }, { "id": "PMID:30365304", "title": "Biochemical Characterization and Structure-Based Mutational Analysis Provide Insight into the Binding and Mechanism of Action of Novel Aspartate Aminotransferase Inhibitors", "journal": "Biochemistry", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30365304/" } ], "x": 5008.666666666667, "y": 754.9666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70600", "label": "glutamate + NAD(P)+ =\u003e alpha-ketoglutarate + NH4+ + NAD(P)H + H+ (GLUD1,2)", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15134", "RHEA:11613" ], "source_url": "https://reactome.org/content/detail/R-HSA-70600", "summary": "glutamate + NAD(P)+ =\u003e alpha-ketoglutarate + NH4+ + NAD(P)H + H+ (GLUD1,2) is a Reactome release 97 curated reaction event. The source record connects L-Glu, NAD(P)+, H2O to 2OG, NH4+, NAD(P)H in mitochondrial matrix and cross-references RHEA:15134, RHEA:11613.", "equation": "L-Glu + NAD(P)+ + H2O → 2OG + NH4+ + NAD(P)H + H+", "inputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-517495", "reactome_id": "R-ALL-517495", "label": "NAD(P)+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113561", "reactome_id": "R-ALL-113561", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-517496", "reactome_id": "R-ALL-517496", "label": "NAD(P)H", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-glutamate dehydrogenase [NAD(P)+] activity of GLUD hexamer [mitochondrial matrix]", "activity_id": "70584", "ec_numbers": [ "1.4.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:11903050", "title": "Expression, purification and characterization of human glutamate dehydrogenase (GDH) allosteric regulatory mutations", "journal": "Biochem J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11903050/" }, { "id": "PMID:429360", "title": "Partial amino acid sequence of the glutamate dehydrogenase of human liver and a revision of the sequence of the bovine enzyme", "journal": "J Biol Chem", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/429360/" }, { "id": "PMID:12054821", "title": "The structure of apo human glutamate dehydrogenase details subunit communication and allostery", "journal": "J Mol Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12054821/" } ], "x": 7461.333333333333, "y": 1607.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70606", "label": "glutamate + NH4+ + ATP =\u003e glutamine + ADP + orthophosphate [GLUL]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16170" ], "source_url": "https://reactome.org/content/detail/R-HSA-70606", "summary": "glutamate + NH4+ + ATP =\u003e glutamine + ADP + orthophosphate [GLUL] is a Reactome release 97 curated reaction event. The source record connects ATP, L-Glu, NH4+ to ADP, L-Gln, Pi in cytosol and cross-references RHEA:16170.", "equation": "ATP + L-Glu + NH4+ → ADP + L-Gln + Pi + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glutamine synthetase activity of GLUL decamer [cytosol]", "activity_id": "70605", "ec_numbers": [ "6.3.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:16267323", "title": "Congenital glutamine deficiency with glutamine synthetase mutations", "journal": "N Engl J Med", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16267323/" }, { "id": "PMID:18005987", "title": "Crystal structures of mammalian glutamine synthetases illustrate substrate-induced conformational changes and provide opportunities for drug and herbicide design", "journal": "J Mol Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18005987/" } ], "x": 7740, "y": 1547.95, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70609", "label": "glutamine + H2O =\u003e glutamate + NH4+ (GLS)", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15890" ], "source_url": "https://reactome.org/content/detail/R-HSA-70609", "summary": "glutamine + H2O =\u003e glutamate + NH4+ (GLS) is a Reactome release 97 curated reaction event. The source record connects H2O, L-Gln to L-Glu, NH4+ in mitochondrial matrix and cross-references RHEA:15890.", "equation": "H2O + L-Gln → L-Glu + NH4+", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113522", "reactome_id": "R-ALL-113522", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113561", "reactome_id": "R-ALL-113561", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutaminase activity of GLS dimers [mitochondrial matrix]", "activity_id": "70608", "ec_numbers": [ "3.5.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11015561", "title": "Cloning and analysis of unique human glutaminase isoforms generated by tissue-specific alternative splicing", "journal": "Physiol Genomics", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/11015561/" }, { "id": "PMID:10620514", "title": "Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase", "journal": "Biochem J", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10620514/" }, { "id": "PMID:3214421", "title": "Purification of phosphate-dependent glutaminase from isolated mitochondria of Ehrlich ascites-tumour cells", "journal": "Biochem J", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3214421/" } ], "x": 8018.666666666667, "y": 1607.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70613", "label": "GOT2 transaminates OA and L-Glu", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport", "glutamate-glutamine", "malate-aspartate-shuttle" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:21826" ], "source_url": "https://reactome.org/content/detail/R-HSA-70613", "summary": "GOT2 transaminates OA and L-Glu is a Reactome release 97 curated reaction event. The source record connects OA, L-Glu to L-Asp, 2OG in mitochondrial matrix and cross-references RHEA:21826.", "equation": "OA + L-Glu → L-Asp + 2OG", "inputs": [ { "node_id": "R-ALL-113587", "reactome_id": "R-ALL-113587", "label": "OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113553", "reactome_id": "R-ALL-113553", "label": "L-Asp", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-aspartate:2-oxoglutarate transaminase activity of GOT2 dimer [mitochondrial matrix]", "activity_id": "70595", "ec_numbers": [ "2.6.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:4052435", "title": "The primary structure of mitochondrial aspartate aminotransferase from human heart", "journal": "Biochim Biophys Acta", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/4052435/" }, { "id": "PMID:31422819", "title": "Bi-allelic GOT2 Mutations Cause a Treatable Malate-Aspartate Shuttle-Related Encephalopathy", "journal": "Am J Hum Genet", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31422819/" }, { "id": "PMID:26902786", "title": "Recombinant expression, purification and crystallographic studies of the mature form of human mitochondrial aspartate aminotransferase", "journal": "Biosci Trends", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26902786/" } ], "x": 4451.333333333333, "y": 780.9, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70634", "label": "ornithine (cytosolic) + citrulline (mitochondrial) =\u003e ornithine (mitochondrial) + citrulline (cytosolic)", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "cytosol · mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:70787" ], "source_url": "https://reactome.org/content/detail/R-HSA-70634", "summary": "ornithine (cytosolic) + citrulline (mitochondrial) =\u003e ornithine (mitochondrial) + citrulline (cytosolic) is a Reactome release 97 curated reaction event. The source record connects L-Cit, L-Orn, H+ to L-Cit, L-Orn, H+ in cytosol · mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:70787.", "equation": "L-Cit + L-Orn + H+ → L-Cit + L-Orn + H+", "inputs": [ { "node_id": "R-ALL-113567", "reactome_id": "R-ALL-113567", "label": "L-Cit", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57743", "formula": "C6H13N3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29498", "reactome_id": "R-ALL-29498", "label": "L-Orn", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46911", "formula": "C5H13N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29968", "reactome_id": "R-ALL-29968", "label": "L-Cit", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57743", "formula": "C6H13N3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113585", "reactome_id": "R-ALL-113585", "label": "L-Orn", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:46911", "formula": "C5H13N2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-ornithine transmembrane transporter activity of Mitochondrial ornithine transporters [mitochondrial inner membrane]", "activity_id": "70633", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10369256", "title": "Hyperornithinaemia-hyperammonaemia-homocitrullinuria syndrome is caused by mutations in a gene encoding a mitochondrial ornithine transporter.", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10369256/" }, { "id": "PMID:12807890", "title": "The mitochondrial ornithine transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution of two human isoforms", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12807890/" }, { "id": "PMID:12948741", "title": "Cloning and characterization of human ORNT2: a second mitochondrial ornithine transporter that can rescue a defective ORNT1 in patients with the hyperornithinemia-hyperammonemia-homocitrullinuria syndrome, a urea cycle disorder", "journal": "Mol Genet Metab", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12948741/" } ], "x": 1530, "y": 3217.888888888889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70654", "label": "ornithine + alpha-ketoglutarate glutamate + L-glutamate gamma-semialdehyde [OAT]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:25161" ], "source_url": "https://reactome.org/content/detail/R-HSA-70654", "summary": "ornithine + alpha-ketoglutarate glutamate + L-glutamate gamma-semialdehyde [OAT] is a Reactome release 97 curated reaction event. The source record connects L-Orn, 2OG to L-Glu, L-Glu5S in mitochondrial matrix and cross-references RHEA:25161.", "equation": "L-Orn + 2OG → L-Glu + L-Glu5S", "inputs": [ { "node_id": "R-ALL-113585", "reactome_id": "R-ALL-113585", "label": "L-Orn", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46911", "formula": "C5H13N2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31339", "reactome_id": "R-ALL-31339", "label": "L-Glu5S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58066", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-ornithine transaminase activity of OAT hexamer [mitochondrial matrix]", "activity_id": "70653", "ec_numbers": [ "2.6.1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:1737786", "title": "Ornithine delta-aminotransferase mutations in gyrate atrophy. Allelic heterogeneity and functional consequences.", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1737786/" }, { "id": "PMID:6819292", "title": "Crystallization and properties of human liver ornithine aminotransferase", "journal": "J Biochem", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/6819292/" }, { "id": "PMID:9514741", "title": "Crystal structure of human recombinant ornithine aminotransferase.", "journal": "J Mol Biol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9514741/" } ], "x": 7461.333333333333, "y": 1637.65, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70655", "label": "L-glutamate gamma-semialdehyde L-1-pyrroline-5-carboxylate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:28235" ], "source_url": "https://reactome.org/content/detail/R-HSA-70655", "summary": "L-glutamate gamma-semialdehyde L-1-pyrroline-5-carboxylate is a Reactome release 97 curated reaction event. The source record connects L-Glu5S to 1PYR-5COOH, H2O, H+ in mitochondrial matrix and cross-references RHEA:28235.", "equation": "L-Glu5S → 1PYR-5COOH + H2O + H+", "inputs": [ { "node_id": "R-ALL-31339", "reactome_id": "R-ALL-31339", "label": "L-Glu5S", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58066", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113590", "reactome_id": "R-ALL-113590", "label": "1PYR-5COOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17388", "formula": "C5H6NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [], "x": 7461.333333333333, "y": 1667.55, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70664", "label": "PYCR1 decamer reduces (S)-1-pyrroline-5-carboxylate to L-Pro", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:14107" ], "source_url": "https://reactome.org/content/detail/R-HSA-70664", "summary": "PYCR1 decamer reduces (S)-1-pyrroline-5-carboxylate to L-Pro is a Reactome release 97 curated reaction event. The source record connects 1PYR-5COOH, H+, NADH to L-Pro, NAD+ in mitochondrial matrix and cross-references RHEA:14107.", "equation": "1PYR-5COOH + 2 H+ + NADH → L-Pro + NAD+", "inputs": [ { "node_id": "R-ALL-113590", "reactome_id": "R-ALL-113590", "label": "1PYR-5COOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17388", "formula": "C5H6NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-379717", "reactome_id": "R-ALL-379717", "label": "L-Pro", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60039", "formula": "C5H9NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyrroline-5-carboxylate reductase activity of PYCR1 decamer [mitochondrial matrix]", "activity_id": "70663", "ec_numbers": [ "1.5.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:23024808", "title": "Functional specialization in proline biosynthesis of melanoma", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23024808/" }, { "id": "PMID:16730026", "title": "Crystal structure of human pyrroline-5-carboxylate reductase", "journal": "J Mol Biol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16730026/" }, { "id": "PMID:19648921", "title": "Mutations in PYCR1 cause cutis laxa with progeroid features", "journal": "Nat Genet", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19648921/" } ], "x": 7740, "y": 1607.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70666", "label": "glutamate + L-glutamate gamma-semialdehyde ornithine + alpha-ketoglutarate [OAT]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:25162" ], "source_url": "https://reactome.org/content/detail/R-HSA-70666", "summary": "glutamate + L-glutamate gamma-semialdehyde ornithine + alpha-ketoglutarate [OAT] is a Reactome release 97 curated reaction event. The source record connects L-Glu, L-Glu5S to L-Orn, 2OG in mitochondrial matrix and cross-references RHEA:25162.", "equation": "L-Glu + L-Glu5S → L-Orn + 2OG", "inputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31339", "reactome_id": "R-ALL-31339", "label": "L-Glu5S", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58066", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113585", "reactome_id": "R-ALL-113585", "label": "L-Orn", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:46911", "formula": "C5H13N2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-ornithine transaminase activity of OAT hexamer [mitochondrial matrix]", "activity_id": "70653", "ec_numbers": [ "2.6.1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:1737786", "title": "Ornithine delta-aminotransferase mutations in gyrate atrophy. Allelic heterogeneity and functional consequences.", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1737786/" } ], "x": 7740, "y": 1637.65, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70667", "label": "1PYR-5COOH spontaneously hydrolyses to L-GluSS", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "proline" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:28236" ], "source_url": "https://reactome.org/content/detail/R-HSA-70667", "summary": "1PYR-5COOH spontaneously hydrolyses to L-GluSS is a Reactome release 97 curated reaction event. The source record connects 1PYR-5COOH, H2O to L-Glu5S in mitochondrial matrix and cross-references RHEA:28236.", "equation": "1PYR-5COOH + H2O → L-Glu5S", "inputs": [ { "node_id": "R-ALL-113590", "reactome_id": "R-ALL-113590", "label": "1PYR-5COOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17388", "formula": "C5H6NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31339", "reactome_id": "R-ALL-31339", "label": "L-Glu5S", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58066", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [], "x": 7078.666666666667, "y": 2288.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70670", "label": "PRODH oxidises L-Pro to 1PYR-5COOH", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "proline" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:23785" ], "source_url": "https://reactome.org/content/detail/R-HSA-70670", "summary": "PRODH oxidises L-Pro to 1PYR-5COOH is a Reactome release 97 curated reaction event. The source record connects L-Pro, FAD to 1PYR-5COOH, FADH2, H+ in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:23785.", "equation": "L-Pro + FAD → 1PYR-5COOH + FADH2 + 2 H+", "inputs": [ { "node_id": "R-ALL-379717", "reactome_id": "R-ALL-379717", "label": "L-Pro", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60039", "formula": "C5H9NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113597", "reactome_id": "R-ALL-113597", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113590", "reactome_id": "R-ALL-113590", "label": "1PYR-5COOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17388", "formula": "C5H6NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-164934", "reactome_id": "R-ALL-164934", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-NH group of donors, quinone or similar compound as acceptor of PRODH [mitochondrial inner membrane]", "activity_id": "70669", "ec_numbers": [ "1.5.5" ], "accessions": [ "O43272" ] } ], "citations": [ { "id": "PMID:15662599", "title": "Functional consequences of PRODH missense mutations", "journal": "Am J Hum Genet", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15662599/" } ], "x": 6521.333333333333, "y": 2363.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70679", "label": "ALDH4A1 oxidises L-GluSS to Glu", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "proline" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:30236" ], "source_url": "https://reactome.org/content/detail/R-HSA-70679", "summary": "ALDH4A1 oxidises L-GluSS to Glu is a Reactome release 97 curated reaction event. The source record connects L-Glu5S, NAD+, H2O to L-Glu, NADH, H+ in mitochondrial matrix and cross-references RHEA:30236.", "equation": "L-Glu5S + NAD+ + H2O → L-Glu + NADH + 2 H+", "inputs": [ { "node_id": "R-ALL-31339", "reactome_id": "R-ALL-31339", "label": "L-Glu5S", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58066", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-glutamate gamma-semialdehyde dehydrogenase activity of ALDH4A1 dimer [mitochondrial matrix]", "activity_id": "70678", "ec_numbers": [ "1.2.1.88" ], "accessions": [] } ], "citations": [ { "id": "PMID:3944130", "title": "Purification and characterization of human liver \"high Km\" aldehyde dehydrogenase and its identification as glutamic gamma-semialdehyde dehydrogenase.", "journal": "J Biol Chem", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3944130/" }, { "id": "PMID:9700195", "title": "Mutations in the Delta1-pyrroline 5-carboxylate dehydrogenase gene cause type II hyperprolinemia.", "journal": "Hum Mol Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9700195/" } ], "x": 6521.333333333333, "y": 2288.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70723", "label": "leu, ile, or val + alpha-ketoglutarate a-ketoisocaproate, a-keto-b-methylvalerate, or a-ketoisovalerate + glutamate [BCAT1]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18322", "RHEA:24802", "RHEA:24814" ], "source_url": "https://reactome.org/content/detail/R-HSA-70723", "summary": "leu, ile, or val + alpha-ketoglutarate a-ketoisocaproate, a-keto-b-methylvalerate, or a-ketoisovalerate + glutamate [BCAT1] is a Reactome release 97 curated reaction event. The source record connects Leu, Ile, Val, 2OG to KIC,KMVA,KIV, L-Glu in cytosol and cross-references RHEA:18322, RHEA:24802, RHEA:24814.", "equation": "Leu, Ile, Val + 2OG → KIC,KMVA,KIV + L-Glu", "inputs": [ { "node_id": "R-ALL-508182", "reactome_id": "R-ALL-508182", "label": "Leu, Ile, Val", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-508187", "reactome_id": "R-ALL-508187", "label": "KIC,KMVA,KIV", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "branched-chain-amino-acid:2-oxoglutarate transaminase activity of BCAT1 dimer [cytosol]", "activity_id": "70700", "ec_numbers": [ "2.6.1.42" ], "accessions": [] } ], "citations": [ { "id": "PMID:16141215", "title": "Structural determinants for branched-chain aminotransferase isozyme-specific inhibition by the anticonvulsant drug gabapentin", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16141215/" } ], "x": 6800, "y": 1557.9166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70724", "label": "leu, ile, or val + alpha-ketoglutarate a-ketoisocaproate, a-keto-b-methylvalerate, or a-ketoisovalerate + glutamate [BCAT2]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24814", "RHEA:18322", "RHEA:24802" ], "source_url": "https://reactome.org/content/detail/R-HSA-70724", "summary": "leu, ile, or val + alpha-ketoglutarate a-ketoisocaproate, a-keto-b-methylvalerate, or a-ketoisovalerate + glutamate [BCAT2] is a Reactome release 97 curated reaction event. The source record connects Leu, Ile, Val, 2OG to KIC, KMVA, KIV, L-Glu in mitochondrial matrix and cross-references RHEA:24814, RHEA:18322, RHEA:24802.", "equation": "Leu, Ile, Val + 2OG → KIC, KMVA, KIV + L-Glu", "inputs": [ { "node_id": "R-ALL-508190", "reactome_id": "R-ALL-508190", "label": "Leu, Ile, Val", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-508181", "reactome_id": "R-ALL-508181", "label": "KIC, KMVA, KIV", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "branched-chain-amino-acid:2-oxoglutarate transaminase activity of BCAT2 dimer [mitochondrial matrix]", "activity_id": "70711", "ec_numbers": [ "2.6.1.42" ], "accessions": [] } ], "citations": [ { "id": "PMID:9165094", "title": "Cloning of the rat and human mitochondrial branched chain aminotransferases (BCATm)", "journal": "Biochim Biophys Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9165094/" }, { "id": "PMID:11264579", "title": "The structure of human mitochondrial branched-chain aminotransferase.", "journal": "Acta Crystallogr D Biol Crystallogr", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11264579/" }, { "id": "PMID:12269802", "title": "Crystal structures of human mitochondrial branched chain aminotransferase reaction intermediates: ketimine and pyridoxamine phosphate forms", "journal": "Biochemistry", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12269802/" } ], "x": 7078.666666666667, "y": 1541.3055555555557, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70745", "label": "isovaleryl-CoA + FAD =\u003e beta-methylcrotonyl-CoA + FADH2", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12277" ], "source_url": "https://reactome.org/content/detail/R-HSA-70745", "summary": "isovaleryl-CoA + FAD =\u003e beta-methylcrotonyl-CoA + FADH2 is a Reactome release 97 curated reaction event. The source record connects ISV-CoA, FAD, H+ to bMC-CoA, FADH2 in mitochondrial matrix and cross-references RHEA:12277.", "equation": "ISV-CoA + FAD + H+ → bMC-CoA + FADH2", "inputs": [ { "node_id": "R-ALL-70715", "reactome_id": "R-ALL-70715", "label": "ISV-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57345", "formula": "C26H40N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70744", "reactome_id": "R-ALL-70744", "label": "bMC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57344", "formula": "C26H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-methylbutanoyl-CoA dehydrogenase activity of IVD tetramer [mitochondrial matrix]", "activity_id": "70743", "ec_numbers": [ "1.3.8.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:3597357", "title": "Purification and properties of short chain acyl-CoA, medium chain acyl-CoA, and isovaleryl-CoA dehydrogenases from human liver.", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3597357/" }, { "id": "PMID:6928646", "title": "Demonstration of a specific mitochondrial isovaleryl-CoA dehydrogenase deficiency in fibroblasts from patients with isovaleric acidemia.", "journal": "Proc Natl Acad Sci U S A", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6928646/" }, { "id": "PMID:9214289", "title": "Structure of human isovaleryl-CoA dehydrogenase at 2.6 A resolution: structural basis for substrate specificity,.", "journal": "Biochemistry", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9214289/" } ], "x": 7078.666666666667, "y": 1624.361111111111, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70773", "label": "beta-methylcrotonyl-CoA + ATP + HCO3- beta-methylglutaconyl-CoA + ADP + orthophosphate + H2O (MCCA)", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:13590" ], "source_url": "https://reactome.org/content/detail/R-HSA-70773", "summary": "beta-methylcrotonyl-CoA + ATP + HCO3- beta-methylglutaconyl-CoA + ADP + orthophosphate + H2O (MCCA) is a Reactome release 97 curated reaction event. The source record connects bMC-CoA, ATP, HCO3- to bMG-CoA, ADP, Pi in mitochondrial matrix and cross-references RHEA:13590.", "equation": "bMC-CoA + ATP + HCO3- → bMG-CoA + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-70744", "reactome_id": "R-ALL-70744", "label": "bMC-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57344", "formula": "C26H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29896", "reactome_id": "R-ALL-29896", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70772", "reactome_id": "R-ALL-70772", "label": "bMG-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57346", "formula": "C27H37N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "methylcrotonoyl-CoA carboxylase activity of 6x(Btn-MCCC1:MCCC2) [mitochondrial matrix]", "activity_id": "70771", "ec_numbers": [ "6.4.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:11181649", "title": "The molecular basis of human 3-methylcrotonyl-CoA carboxylase deficiency.", "journal": "J Clin Invest", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11181649/" }, { "id": "PMID:7356336", "title": "Subcellular localization of 3-methylcrotonyl-coenzyme A carboxylase in bovine kidney", "journal": "Arch Biochem Biophys", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/7356336/" }, { "id": "PMID:11406611", "title": "Cloning of the human MCCA and MCCB genes and mutations therein reveal the molecular cause of 3-methylcrotonyl-CoA: carboxylase deficiency.", "journal": "Hum Mol Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11406611/" }, { "id": "PMID:16023992", "title": "Mitochondrial targeting signals and mature peptides of 3-methylcrotonyl-CoA carboxylase", "journal": "Biochem Biophys Res Commun", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16023992/" } ], "x": 6800, "y": 1574.5277777777778, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70785", "label": "beta-methylglutaconyl-CoA + H2O beta-hydroxy-beta-methylglutaryl-CoA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:21538" ], "source_url": "https://reactome.org/content/detail/R-HSA-70785", "summary": "beta-methylglutaconyl-CoA + H2O beta-hydroxy-beta-methylglutaryl-CoA is a Reactome release 97 curated reaction event. The source record connects bMG-CoA, H2O to bHMG-CoA in mitochondrial matrix and cross-references RHEA:21538.", "equation": "bMG-CoA + H2O → bHMG-CoA", "inputs": [ { "node_id": "R-ALL-70772", "reactome_id": "R-ALL-70772", "label": "bMG-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57346", "formula": "C27H37N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70784", "reactome_id": "R-ALL-70784", "label": "bHMG-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43074", "formula": "C27H39N7O20P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methylglutaconyl-CoA hydratase activity of AUH hexamer [mitochondrial matrix]", "activity_id": "70783", "ec_numbers": [ "4.2.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:12434311", "title": "3-Methylglutaconic aciduria type I is caused by mutations in AUH.", "journal": "Am J Hum Genet", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12434311/" }, { "id": "PMID:11738050", "title": "Crystal structure of human AUH protein, a single-stranded RNA binding homolog of enoyl-CoA hydratase", "journal": "Structure", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11738050/" }, { "id": "PMID:3082934", "title": "Deficiency of 3-methylglutaconyl-coenzyme A hydratase in two siblings with 3-methylglutaconic aciduria.", "journal": "J Clin Invest", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3082934/" } ], "x": 7078.666666666667, "y": 1607.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70800", "label": "alpha-methylbutyryl-CoA + FAD =\u003e tiglyl-CoA + FADH2", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:43781" ], "source_url": "https://reactome.org/content/detail/R-HSA-70800", "summary": "alpha-methylbutyryl-CoA + FAD =\u003e tiglyl-CoA + FADH2 is a Reactome release 97 curated reaction event. The source record connects 2MBUT-CoA, FAD, H+ to tiglyl-CoA, FADH2 in mitochondrial matrix and cross-references RHEA:43781.", "equation": "2MBUT-CoA + FAD + H+ → tiglyl-CoA + FADH2", "inputs": [ { "node_id": "R-ALL-70718", "reactome_id": "R-ALL-70718", "label": "2MBUT-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:156439", "formula": "C26H40N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70799", "reactome_id": "R-ALL-70799", "label": "tiglyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57337", "formula": "C26H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADSB tetramer [mitochondrial matrix]", "activity_id": "70798", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:11013134", "title": "Isolated 2-methylbutyrylglycinuria caused by short/branched-chain acyl-CoA dehydrogenase deficiency: identification of a new enzyme defect, resolution of its molecular basis, and evidence for distinct acyl-CoA dehydrogenases in isoleucine and valine metabolism.", "journal": "Am J Hum Genet", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11013134/" }, { "id": "PMID:10832746", "title": "2-Methylbutyryl-coenzyme A dehydrogenase deficiency: a new inborn error of L-isoleucine metabolism.", "journal": "Pediatr Res", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10832746/" } ], "x": 6521.333333333333, "y": 1640.9722222222222, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70830", "label": "tiglyl-CoA + H2O alpha-methyl-beta-hydroxybutyryl-CoA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31121" ], "source_url": "https://reactome.org/content/detail/R-HSA-70830", "summary": "tiglyl-CoA + H2O alpha-methyl-beta-hydroxybutyryl-CoA is a Reactome release 97 curated reaction event. The source record connects tiglyl-CoA, H2O to aMbHBUT-CoA in mitochondrial matrix and cross-references RHEA:31121.", "equation": "tiglyl-CoA + H2O → aMbHBUT-CoA", "inputs": [ { "node_id": "R-ALL-70799", "reactome_id": "R-ALL-70799", "label": "tiglyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57337", "formula": "C26H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70829", "reactome_id": "R-ALL-70829", "label": "aMbHBUT-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57312", "formula": "C26H40N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of enoyl-CoA hydratase [mitochondrial matrix]", "activity_id": "70828", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [], "x": 6521.333333333333, "y": 1624.361111111111, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70837", "label": "alpha-methyl-beta-hydroxybutyryl-CoA + NAD+ alpha-methylacetoacetyl-CoA + NADH + H+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:13282" ], "source_url": "https://reactome.org/content/detail/R-HSA-70837", "summary": "alpha-methyl-beta-hydroxybutyryl-CoA + NAD+ alpha-methylacetoacetyl-CoA + NADH + H+ is a Reactome release 97 curated reaction event. The source record connects aMbHBUT-CoA, NAD+ to 2MACA-CoA, NADH, H+ in mitochondrial matrix and cross-references RHEA:13282.", "equation": "aMbHBUT-CoA + NAD+ → 2MACA-CoA + NADH + H+", "inputs": [ { "node_id": "R-ALL-70829", "reactome_id": "R-ALL-70829", "label": "aMbHBUT-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57312", "formula": "C26H40N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70836", "reactome_id": "R-ALL-70836", "label": "2MACA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57335", "formula": "C26H38N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of HSD17B10 tetramer [mitochondrial matrix]", "activity_id": "70835", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:15342248", "title": "Crystal structure of human ABAD/HSD10 with a bound inhibitor: implications for design of Alzheimer's disease therapeutics", "journal": "J Mol Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15342248/" }, { "id": "PMID:12696021", "title": "2-Methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency is caused by mutations in the HADH2 gene.", "journal": "Am J Hum Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12696021/" } ], "x": 6800, "y": 1591.138888888889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70844", "label": "alpha-methylacetoacetyl-CoA + CoA =\u003e propionyl-CoA + acetyl-CoA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:30721" ], "source_url": "https://reactome.org/content/detail/R-HSA-70844", "summary": "alpha-methylacetoacetyl-CoA + CoA =\u003e propionyl-CoA + acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects 2MACA-CoA, CoA-SH to PROP-CoA, Ac-CoA in mitochondrial matrix and cross-references RHEA:30721.", "equation": "2MACA-CoA + CoA-SH → PROP-CoA + Ac-CoA", "inputs": [ { "node_id": "R-ALL-70836", "reactome_id": "R-ALL-70836", "label": "2MACA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57335", "formula": "C26H38N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70843", "reactome_id": "R-ALL-70843", "label": "PROP-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57392", "formula": "C24H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acetyltransferase activity of ACAT1 tetramer [mitochondrial matrix]", "activity_id": "70842", "ec_numbers": [ "2.3.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:17371050", "title": "Crystallographic and kinetic studies of human mitochondrial acetoacetyl-CoA thiolase: the importance of potassium and chloride ions for its structure and function", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17371050/" } ], "x": 6800, "y": 1607.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70859", "label": "isobutyryl-CoA + FAD =\u003e methacrylyl-CoA + FADH2", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:44181" ], "source_url": "https://reactome.org/content/detail/R-HSA-70859", "summary": "isobutyryl-CoA + FAD =\u003e methacrylyl-CoA + FADH2 is a Reactome release 97 curated reaction event. The source record connects ISB-CoA, FAD, H+ to MACR-CoA, FADH2 in mitochondrial matrix and cross-references RHEA:44181.", "equation": "ISB-CoA + FAD + H+ → MACR-CoA + FADH2", "inputs": [ { "node_id": "R-ALL-70851", "reactome_id": "R-ALL-70851", "label": "ISB-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57338", "formula": "C25H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70858", "reactome_id": "R-ALL-70858", "label": "MACR-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62500", "formula": "C25H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACAD8 tetramer [mitochondrial matrix]", "activity_id": "70857", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:14752098", "title": "Structures of isobutyryl-CoA dehydrogenase and enzyme-product complex: comparison with isovaleryl- and short-chain acyl-CoA dehydrogenases", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14752098/" }, { "id": "PMID:9889013", "title": "Isolated isobutyryl-CoA dehydrogenase deficiency: an unrecognized defect in human valine metabolism.", "journal": "Mol Genet Metab", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9889013/" }, { "id": "PMID:12359132", "title": "Identification of isobutyryl-CoA dehydrogenase and its deficiency in humans.", "journal": "Mol Genet Metab", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359132/" } ], "x": 6800, "y": 1640.9722222222222, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70870", "label": "ECHS1 hydrates methacrylyl-CoA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31176" ], "source_url": "https://reactome.org/content/detail/R-HSA-70870", "summary": "ECHS1 hydrates methacrylyl-CoA is a Reactome release 97 curated reaction event. The source record connects MACR-CoA, H2O to bHIB-CoA in mitochondrial matrix and cross-references RHEA:31176.", "equation": "MACR-CoA + H2O → bHIB-CoA", "inputs": [ { "node_id": "R-ALL-70858", "reactome_id": "R-ALL-70858", "label": "MACR-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62500", "formula": "C25H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-71847", "reactome_id": "R-ALL-71847", "label": "bHIB-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62611", "formula": "C25H38N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of ECHS1 hexamer [mitochondrial matrix]", "activity_id": "71049", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:26251176", "title": "Clinical, biochemical and metabolic characterisation of a mild form of human short-chain enoyl-CoA hydratase deficiency: significance of increased N-acetyl-S-(2-carboxypropyl)cysteine excretion", "journal": "J. Med. Genet.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26251176/" } ], "x": 6800, "y": 1624.361111111111, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70881", "label": "beta-hydroxyisobutyryl-CoA + H2O =\u003e beta-hydroxyisobutyrate + CoA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31252" ], "source_url": "https://reactome.org/content/detail/R-HSA-70881", "summary": "beta-hydroxyisobutyryl-CoA + H2O =\u003e beta-hydroxyisobutyrate + CoA is a Reactome release 97 curated reaction event. The source record connects bHIB-CoA, H2O to bHIBA, CoA-SH, H+ in mitochondrial matrix and cross-references RHEA:31252.", "equation": "bHIB-CoA + H2O → bHIBA + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-71847", "reactome_id": "R-ALL-71847", "label": "bHIB-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62611", "formula": "C25H38N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-573404", "reactome_id": "R-ALL-573404", "label": "bHIBA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62638", "formula": "C4H7O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-hydroxyisobutyryl-CoA hydrolase activity of HIBCH [mitochondrial matrix]", "activity_id": "70880", "ec_numbers": [ "3.1.2.4" ], "accessions": [ "Q6NVY1" ] } ], "citations": [ { "id": "PMID:8824301", "title": "Primary structure and tissue-specific expression of human beta-hydroxyisobutyryl-coenzyme A hydrolase.", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8824301/" } ], "x": 7078.666666666667, "y": 1591.138888888889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70885", "label": "beta-hydroxyisobutyrate + NAD+ methylmalonyl semialdehyde + NADH + H+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31276" ], "source_url": "https://reactome.org/content/detail/R-HSA-70885", "summary": "beta-hydroxyisobutyrate + NAD+ methylmalonyl semialdehyde + NADH + H+ is a Reactome release 97 curated reaction event. The source record connects bHIBA, NAD+ to 2M3OPROA, NADH, H+ in mitochondrial matrix and cross-references RHEA:31276.", "equation": "bHIBA + NAD+ → 2M3OPROA + NADH + H+", "inputs": [ { "node_id": "R-ALL-573404", "reactome_id": "R-ALL-573404", "label": "bHIBA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62638", "formula": "C4H7O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30004", "reactome_id": "R-ALL-30004", "label": "2M3OPROA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62413", "formula": "C4H5O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-hydroxyisobutyrate dehydrogenase activity of HIBADH tetramer [mitochondrial matrix]", "activity_id": "70884", "ec_numbers": [ "1.1.1.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:13416257", "title": "Purification and properties of beta-hydroxyisobutyric dehydrogenase", "journal": "J Biol Chem", "year": 1957, "url": "https://pubmed.ncbi.nlm.nih.gov/13416257/" } ], "x": 6521.333333333333, "y": 1607.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70893", "label": "methylmalonate semialdehyde + NAD+ + CoA + H2O =\u003e propionyl-CoA + HCO3- + NADH + H+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:76628" ], "source_url": "https://reactome.org/content/detail/R-HSA-70893", "summary": "methylmalonate semialdehyde + NAD+ + CoA + H2O =\u003e propionyl-CoA + HCO3- + NADH + H+ is a Reactome release 97 curated reaction event. The source record connects 2M3OPROA, NAD+, CoA-SH to PROP-CoA, NADH, H+ in mitochondrial matrix and cross-references RHEA:76628.", "equation": "2M3OPROA + NAD+ + CoA-SH + H2O → PROP-CoA + NADH + H+ + HCO3-", "inputs": [ { "node_id": "R-ALL-30004", "reactome_id": "R-ALL-30004", "label": "2M3OPROA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62413", "formula": "C4H5O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70843", "reactome_id": "R-ALL-70843", "label": "PROP-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57392", "formula": "C24H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29896", "reactome_id": "R-ALL-29896", "label": "HCO3-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity of ALDH6A1 [mitochondrial matrix]", "activity_id": "70892", "ec_numbers": [ "1.2.1.27" ], "accessions": [ "Q02252" ] } ], "citations": [ { "id": "PMID:10947204", "title": "Molecular characterization of methylmalonate semialdehyde dehydrogenase deficiency", "journal": "J Inherit Metab Dis", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10947204/" }, { "id": "PMID:1527093", "title": "CoA-dependent methylmalonate-semialdehyde dehydrogenase, a unique member of the aldehyde dehydrogenase superfamily. cDNA cloning, evolutionary relationships, and tissue distribution", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1527093/" } ], "x": 7078.666666666667, "y": 1557.9166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70899", "label": "histidine =\u003e urocanate + NH4+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21233" ], "source_url": "https://reactome.org/content/detail/R-HSA-70899", "summary": "histidine =\u003e urocanate + NH4+ is a Reactome release 97 curated reaction event. The source record connects L-His to UCA, NH4+ in cytosol and cross-references RHEA:21233.", "equation": "L-His → UCA + NH4+", "inputs": [ { "node_id": "R-ALL-29612", "reactome_id": "R-ALL-29612", "label": "L-His", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57595", "formula": "C6H9N3O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30721", "reactome_id": "R-ALL-30721", "label": "UCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17771", "formula": "C6H5N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "histidine ammonia-lyase activity of HAL [cytosol]", "activity_id": "70898", "ec_numbers": [ "4.3.1.3" ], "accessions": [ "P42357" ] } ], "citations": [ { "id": "PMID:15806399", "title": "Molecular characterization of histidinemia: identification of four missense mutations in the histidase gene", "journal": "Hum Genet", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15806399/" } ], "x": 8018.666666666667, "y": 2068.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70903", "label": "urocanate + H2O =\u003e 4-imidazolone-5-propanoate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13103" ], "source_url": "https://reactome.org/content/detail/R-HSA-70903", "summary": "urocanate + H2O =\u003e 4-imidazolone-5-propanoate is a Reactome release 97 curated reaction event. The source record connects UCA, H2O to 4I-5PROA in cytosol and cross-references RHEA:13103.", "equation": "UCA + H2O → 4I-5PROA", "inputs": [ { "node_id": "R-ALL-30721", "reactome_id": "R-ALL-30721", "label": "UCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17771", "formula": "C6H5N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-34979", "reactome_id": "R-ALL-34979", "label": "4I-5PROA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77893", "formula": "C6H7N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "urocanate hydratase activity of UROC1:NAD+ [cytosol]", "activity_id": "70902", "ec_numbers": [ "4.2.1.49" ], "accessions": [] } ], "citations": [ { "id": "PMID:19304569", "title": "Mutations in the urocanase gene UROC1 are associated with urocanic aciduria", "journal": "J Med Genet", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19304569/" }, { "id": "PMID:15221005", "title": "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas", "journal": "Oncogene", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15221005/" } ], "x": 7461.333333333333, "y": 2105.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70906", "label": "4-imidazolone-5-propanoate + H2O =\u003e N-formiminoglutamate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23661" ], "source_url": "https://reactome.org/content/detail/R-HSA-70906", "summary": "4-imidazolone-5-propanoate + H2O =\u003e N-formiminoglutamate is a Reactome release 97 curated reaction event. The source record connects 4I-5PROA, H2O to NF-Glu in cytosol and cross-references RHEA:23661.", "equation": "4I-5PROA + H2O → NF-Glu", "inputs": [ { "node_id": "R-ALL-34979", "reactome_id": "R-ALL-34979", "label": "4I-5PROA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77893", "formula": "C6H7N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30158", "reactome_id": "R-ALL-30158", "label": "NF-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58928", "formula": "C6H9N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in cyclic amides of AMDHD1 [cytosol]", "activity_id": "70905", "ec_numbers": [ "3.5.2" ], "accessions": [ "Q96NU7" ] } ], "citations": [ { "id": "PMID:15221005", "title": "Expression profiling and differential screening between hepatoblastomas and the corresponding normal livers: identification of high expression of the PLK1 oncogene as a poor-prognostic indicator of hepatoblastomas", "journal": "Oncogene", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15221005/" } ], "x": 7740, "y": 2105.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70920", "label": "N-formiminoglutamate + tetrahydrofolate =\u003e glutamate + 5-formiminotetrahydrofolate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15099" ], "source_url": "https://reactome.org/content/detail/R-HSA-70920", "summary": "N-formiminoglutamate + tetrahydrofolate =\u003e glutamate + 5-formiminotetrahydrofolate is a Reactome release 97 curated reaction event. The source record connects NF-Glu, THF to L-Glu, FITHF in cytosol and cross-references RHEA:15099.", "equation": "NF-Glu + THF → L-Glu + FITHF", "inputs": [ { "node_id": "R-ALL-30158", "reactome_id": "R-ALL-30158", "label": "NF-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58928", "formula": "C6H9N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111355", "reactome_id": "R-ALL-111355", "label": "THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70919", "reactome_id": "R-ALL-70919", "label": "FITHF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57456", "formula": "C20H22N8O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "formimidoyltetrahydrofolate cyclodeaminase activity of FTCD octamer [cytosol]", "activity_id": "70917", "ec_numbers": [ "4.3.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:12815595", "title": "The molecular basis of glutamate formiminotransferase deficiency", "journal": "Hum Mutat", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12815595/" }, { "id": "PMID:7901203", "title": "The nucleotide sequence of porcine formiminotransferase cyclodeaminase. Expression and purification from Escherichia coli.", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7901203/" }, { "id": "PMID:10773664", "title": "Cloning and characterization of human FTCD on 21q22.3, a candidate gene for glutamate formiminotransferase deficiency.", "journal": "Cytogenet Cell Genet", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10773664/" } ], "x": 7461.333333333333, "y": 2068.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70938", "label": "lysine + alpha-ketoglutarate +NADPH + H+ =\u003e saccharopine + NADP+ + H2O", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:19375" ], "source_url": "https://reactome.org/content/detail/R-HSA-70938", "summary": "lysine + alpha-ketoglutarate +NADPH + H+ =\u003e saccharopine + NADP+ + H2O is a Reactome release 97 curated reaction event. The source record connects L-Lys, 2OG, NADPH to SACN, NADP+, H2O in mitochondrial matrix and cross-references RHEA:19375.", "equation": "L-Lys + 2OG + NADPH + H+ → SACN + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-29444", "reactome_id": "R-ALL-29444", "label": "L-Lys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32551", "formula": "C6H15N2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30176", "reactome_id": "R-ALL-30176", "label": "SACN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57951", "formula": "C11H19N2O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "saccharopine dehydrogenase (NADP+, L-lysine-forming) activity of AASS tetramer [mitochondrial matrix]", "activity_id": "70934", "ec_numbers": [ "1.5.1.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:6434529", "title": "Familial hyperlysinemias. Purification and characterization of the bifunctional aminoadipic semialdehyde synthase with lysine-ketoglutarate reductase and saccharopine dehydrogenase activities.", "journal": "J Biol Chem", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6434529/" }, { "id": "PMID:10775527", "title": "Identification of the alpha-aminoadipic semialdehyde synthase gene, which is defective in familial hyperlysinemia.", "journal": "Am J Hum Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/10775527/" } ], "x": 6521.333333333333, "y": 2024.4583333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70940", "label": "saccharopine + NAD+ + H2O =\u003e alpha-aminoadipic semialdehyde + glutamate + NADH + H+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24521" ], "source_url": "https://reactome.org/content/detail/R-HSA-70940", "summary": "saccharopine + NAD+ + H2O =\u003e alpha-aminoadipic semialdehyde + glutamate + NADH + H+ is a Reactome release 97 curated reaction event. The source record connects SACN, NAD+, H2O to 2AMAS, L-Glu, NADH in mitochondrial matrix and cross-references RHEA:24521.", "equation": "SACN + NAD+ + H2O → 2AMAS + L-Glu + NADH + H+", "inputs": [ { "node_id": "R-ALL-30176", "reactome_id": "R-ALL-30176", "label": "SACN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57951", "formula": "C11H19N2O6", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113556", "reactome_id": "R-ALL-113556", "label": "2AMAS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58321", "formula": "C6H11NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity of AASS tetramer [mitochondrial matrix]", "activity_id": "70937", "ec_numbers": [ "1.5.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:6434529", "title": "Familial hyperlysinemias. Purification and characterization of the bifunctional aminoadipic semialdehyde synthase with lysine-ketoglutarate reductase and saccharopine dehydrogenase activities.", "journal": "J Biol Chem", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6434529/" }, { "id": "PMID:10775527", "title": "Identification of the alpha-aminoadipic semialdehyde synthase gene, which is defective in familial hyperlysinemia.", "journal": "Am J Hum Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/10775527/" } ], "x": 6800, "y": 2024.4583333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70941", "label": "alpha-aminoadipoate semialdehyde + NAD+ =\u003e alpha-aminoadipate + NADH + H+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12309" ], "source_url": "https://reactome.org/content/detail/R-HSA-70941", "summary": "alpha-aminoadipoate semialdehyde + NAD+ =\u003e alpha-aminoadipate + NADH + H+ is a Reactome release 97 curated reaction event. The source record connects 2AMAS, NAD+, H2O to 2AMA, NADH, H+ in mitochondrial matrix and cross-references RHEA:12309.", "equation": "2AMAS + NAD+ + H2O → 2AMA + NADH + 2 H+", "inputs": [ { "node_id": "R-ALL-113556", "reactome_id": "R-ALL-113556", "label": "2AMAS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58321", "formula": "C6H11NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-508531", "reactome_id": "R-ALL-508531", "label": "2AMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58672", "formula": "C6H10NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-aminoadipate-semialdehyde dehydrogenase [NAD(P)+] activity of ALDH7A1 tetramer [mitochondrial matrix]", "activity_id": "508540", "ec_numbers": [ "1.2.1.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:16491085", "title": "Mutations in antiquitin in individuals with pyridoxine-dependent seizures", "journal": "Nat Med", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16491085/" }, { "id": "PMID:19885858", "title": "Is antiquitin a mitochondrial Enzyme?", "journal": "J Cell Biochem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19885858/" } ], "x": 7078.666666666667, "y": 2024.4583333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70952", "label": "alpha-aminoadipate + alpha-ketoglutarate alpha-ketoadipate + glutamate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12602" ], "source_url": "https://reactome.org/content/detail/R-HSA-70952", "summary": "alpha-aminoadipate + alpha-ketoglutarate alpha-ketoadipate + glutamate is a Reactome release 97 curated reaction event. The source record connects 2OG, 2AMA to L-Glu, 2-OA in mitochondrial matrix and cross-references RHEA:12602.", "equation": "2OG + 2AMA → L-Glu + 2-OA", "inputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-508531", "reactome_id": "R-ALL-508531", "label": "2AMA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58672", "formula": "C6H10NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70951", "reactome_id": "R-ALL-70951", "label": "2-OA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57499", "formula": "C6H6O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-2-aminoadipate:2-oxoglutarate transaminase activity of AADAT dimer [mitochondrial matrix]", "activity_id": "70950", "ec_numbers": [ "2.6.1.39" ], "accessions": [] } ], "citations": [ { "id": "PMID:12126930", "title": "Characterization of the human gene encoding alpha-aminoadipate aminotransferase (AADAT).", "journal": "Mol Genet Metab", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12126930/" }, { "id": "PMID:18056995", "title": "Crystal structure of human kynurenine aminotransferase II", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18056995/" }, { "id": "PMID:18056996", "title": "Crystal structure of human kynurenine aminotransferase II, a drug target for the treatment of schizophrenia", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18056996/" } ], "x": 6800, "y": 2099.2083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70967", "label": "IDH3 complex decarboxylates isocitrate", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:23633" ], "source_url": "https://reactome.org/content/detail/R-HSA-70967", "summary": "IDH3 complex decarboxylates isocitrate is a Reactome release 97 curated reaction event. The source record connects ISCIT, NAD+ to 2OG, CO2, NADH in mitochondrial matrix and cross-references RHEA:23633.", "equation": "ISCIT + NAD+ → 2OG + CO2 + NADH", "inputs": [ { "node_id": "R-ALL-29938", "reactome_id": "R-ALL-29938", "label": "ISCIT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15562", "formula": "C6H5O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "isocitrate dehydrogenase (NAD+) activity of IDH3 octamer [mitochondrial matrix]", "activity_id": "70966", "ec_numbers": [ "1.1.1.41" ], "accessions": [] } ], "citations": [ { "id": "PMID:17432878", "title": "Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17432878/" }, { "id": "PMID:20435888", "title": "Each conserved active site tyr in the three subunits of human isocitrate dehydrogenase has a different function", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20435888/" }, { "id": "PMID:14555658", "title": "Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14555658/" }, { "id": "PMID:16737955", "title": "Identification of Mn2+-binding aspartates from alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16737955/" }, { "id": "PMID:18806796", "title": "Insights from retinitis pigmentosa into the roles of isocitrate dehydrogenases in the Krebs cycle", "journal": "Nat Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18806796/" }, { "id": "PMID:4336621", "title": "Diphosphopyridine nucleotide dependent isocitrate dehydrogenase from pig heart. Charactgerization of the active substrate and modes of regulation", "journal": "Biochemistry", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/4336621/" } ], "x": 5391.333333333333, "y": 384.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70971", "label": "ACO2 isomerizes citrate", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:10337", "RHEA:10336" ], "source_url": "https://reactome.org/content/detail/R-HSA-70971", "summary": "ACO2 isomerizes citrate is a Reactome release 97 curated reaction event. The source record connects CIT to ISCIT in mitochondrial matrix and cross-references RHEA:10337, RHEA:10336.", "equation": "CIT → ISCIT", "inputs": [ { "node_id": "R-ALL-29654", "reactome_id": "R-ALL-29654", "label": "CIT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16947", "formula": "C6H5O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29938", "reactome_id": "R-ALL-29938", "label": "ISCIT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15562", "formula": "C6H5O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aconitate hydratase activity of ACO2:4Fe-4S [mitochondrial matrix]", "activity_id": "70969", "ec_numbers": [ "4.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:1946331", "title": "Purification and partial amino acid sequence of human aconitase", "journal": "Protein Seq Data Anal", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1946331/" }, { "id": "PMID:1052766", "title": "Aconitase polymorphism in man", "journal": "Ann Hum Genet", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/1052766/" }, { "id": "PMID:34354088", "title": "ACO2 clinicobiological dataset with extensive phenotype ontology annotation", "journal": "Sci Data", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34354088/" } ], "x": 5391.333333333333, "y": 433.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70975", "label": "CS acetylates OA to citrate", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:16846" ], "source_url": "https://reactome.org/content/detail/R-HSA-70975", "summary": "CS acetylates OA to citrate is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, H2O, OA to CIT, CoA-SH, H+ in mitochondrial matrix and cross-references RHEA:16846.", "equation": "Ac-CoA + H2O + OA → CIT + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113587", "reactome_id": "R-ALL-113587", "label": "OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29654", "reactome_id": "R-ALL-29654", "label": "CIT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16947", "formula": "C6H5O7", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "citrate synthase activity of N6,N6,N6-trimethyl-K395-CS dimer [mitochondrial matrix]", "activity_id": "70974", "ec_numbers": [ "2.3.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6824331", "title": "Complexes between mitochondrial enzymes and either citrate synthase or glutamate dehydrogenase", "journal": "Arch Biochem Biophys", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6824331/" }, { "id": "PMID:9809442", "title": "Cloning and molecular analysis of the human citrate synthase gene", "journal": "Genome", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9809442/" }, { "id": "PMID:28887308", "title": "Uncovering human METTL12 as a mitochondrial methyltransferase that modulates citrate synthase activity through metabolite-sensitive lysine methylation", "journal": "J Biol Chem", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28887308/" }, { "id": "PMID:9792662", "title": "Interaction between citrate synthase and malate dehydrogenase. Substrate channeling of oxaloacetate", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9792662/" }, { "id": "PMID:34548590", "title": "Association of the malate dehydrogenase-citrate synthase metabolon is modulated by intermediates of the Krebs tricarboxylic acid cycle", "journal": "Sci Rep", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34548590/" }, { "id": "PMID:25537779", "title": "Krebs cycle metabolon: structural evidence of substrate channeling revealed by cross-linking and mass spectrometry", "journal": "Angew Chem Int Ed Engl", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25537779/" } ], "x": 5948.666666666667, "y": 287.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70979", "label": "MDH2 dimer dehydrogenates MAL", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport", "malate-aspartate-shuttle", "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:21433" ], "source_url": "https://reactome.org/content/detail/R-HSA-70979", "summary": "MDH2 dimer dehydrogenates MAL is a Reactome release 97 curated reaction event. The source record connects MAL, NAD+ to OA, NADH, H+ in mitochondrial matrix and cross-references RHEA:21433.", "equation": "MAL + NAD+ → OA + NADH + H+", "inputs": [ { "node_id": "R-ALL-113544", "reactome_id": "R-ALL-113544", "label": "MAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113587", "reactome_id": "R-ALL-113587", "label": "OA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-malate dehydrogenase (NAD+) activity of MDH2 dimer [mitochondrial matrix]", "activity_id": "70978", "ec_numbers": [ "1.1.1.37" ], "accessions": [] } ], "citations": [ { "id": "PMID:16740313", "title": "An NADH-tetrazolium-coupled sensitive assay for malate dehydrogenase in mitochondria and crude tissue homogenates", "journal": "J Biochem Biophys Methods", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16740313/" }, { "id": "PMID:9792106", "title": "Aggregation states of mitochondrial malate dehydrogenase", "journal": "Protein Sci", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9792106/" }, { "id": "PMID:36139014", "title": "Structural Comparison of hMDH2 Complexed with Natural Substrates and Cofactors: The Importance of Phosphate Binding for Active Conformation and Catalysis", "journal": "Biomolecules", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36139014/" }, { "id": "PMID:36420423", "title": "Malate dehydrogenase 2 deficiency is an emerging cause of pediatric epileptic encephalopathy with a recognizable biochemical signature", "journal": "Mol Genet Metab Rep", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36420423/" }, { "id": "PMID:35366151", "title": "Palmitoylation of MDH2 by ZDHHC18 activates mitochondrial respiration and accelerates ovarian cancer growth", "journal": "Sci China Life Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35366151/" } ], "x": 5670, "y": 384.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70982", "label": "FH tetramer hydrates fumarate to L-malate", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12462", "RHEA:12460" ], "source_url": "https://reactome.org/content/detail/R-HSA-70982", "summary": "FH tetramer hydrates fumarate to L-malate is a Reactome release 97 curated reaction event. The source record connects FUMA, H2O to MAL in mitochondrial matrix and cross-references RHEA:12462, RHEA:12460.", "equation": "FUMA + H2O → MAL", "inputs": [ { "node_id": "R-ALL-113588", "reactome_id": "R-ALL-113588", "label": "FUMA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29806", "formula": "C4H2O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113544", "reactome_id": "R-ALL-113544", "label": "MAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fumarate hydratase activity of FH tetramer [mitochondrial matrix]", "activity_id": "70981", "ec_numbers": [ "4.2.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8200987", "title": "Mutation of the fumarase gene in two siblings with progressive encephalopathy and fumarase deficiency", "journal": "J Clin Invest", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8200987/" }, { "id": "PMID:30761759", "title": "Structural, biochemical and biophysical characterization of recombinant human fumarate hydratase", "journal": "FEBS J", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30761759/" }, { "id": "PMID:37512586", "title": "The Pleiotropic Effects of Fumarate: From Mitochondrial Respiration to Epigenetic Rewiring and DNA Repair Mechanisms", "journal": "Metabolites", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37512586/" }, { "id": "PMID:33058874", "title": "Post-translational Modifications of Fumarase Regulate its Enzyme Activity and Function in Respiration and the DNA Damage Response", "journal": "J Mol Biol", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33058874/" } ], "x": 5948.666666666667, "y": 384.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70994", "label": "SDH complex dehydrogenates succinate", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:13714" ], "source_url": "https://reactome.org/content/detail/R-HSA-70994", "summary": "SDH complex dehydrogenates succinate is a Reactome release 97 curated reaction event. The source record connects SUCCA, CoQ to FUMA, CoQH2 in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:13714.", "equation": "SUCCA + CoQ → FUMA + CoQH2", "inputs": [ { "node_id": "R-ALL-113536", "reactome_id": "R-ALL-113536", "label": "SUCCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30031", "formula": "C4H4O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73663", "reactome_id": "R-ALL-73663", "label": "CoQ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46245", "formula": "C59H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113588", "reactome_id": "R-ALL-113588", "label": "FUMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29806", "formula": "C4H2O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162198", "reactome_id": "R-ALL-2162198", "label": "CoQH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "succinate dehydrogenase (quinone) activity of SDH complex [mitochondrial inner membrane]", "activity_id": "70993", "ec_numbers": [ "1.3.5.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16143825", "title": "Succinate dehydrogenase deficiency in human", "journal": "Cell Mol Life Sci", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16143825/" }, { "id": "PMID:14946179", "title": "The inhibitory effects of itaconic acid in vitro and in vivo", "journal": "J Biol Chem", "year": 1952, "url": "https://pubmed.ncbi.nlm.nih.gov/14946179/" }, { "id": "PMID:14249115", "title": "STUDIES ON SUCCINATE DEHYDROGENASE. I. SPECTRAL PROPERTIES OF THE PURIFIED ENZYME AND FORMATION OF ENZYME-COMPETITIVE INHIBITOR COMPLEXES", "journal": "Biochim Biophys Acta", "year": 1964, "url": "https://pubmed.ncbi.nlm.nih.gov/14249115/" }, { "id": "PMID:27189937", "title": "Immunoresponsive Gene 1 and Itaconate Inhibit Succinate Dehydrogenase to Modulate Intracellular Succinate Levels", "journal": "J Biol Chem", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27189937/" }, { "id": "PMID:27374498", "title": "Itaconate Links Inhibition of Succinate Dehydrogenase with Macrophage Metabolic Remodeling and Regulation of Inflammation", "journal": "Cell Metab", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27374498/" }, { "id": "PMID:15652751", "title": "Succinate links TCA cycle dysfunction to oncogenesis by inhibiting HIF-alpha prolyl hydroxylase", "journal": "Cancer Cell", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15652751/" } ], "x": 5670, "y": 433.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-70997", "label": "SUCLG1/A2 cleaves succinyl-CoA", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:17663", "RHEA:17662" ], "source_url": "https://reactome.org/content/detail/R-HSA-70997", "summary": "SUCLG1/A2 cleaves succinyl-CoA is a Reactome release 97 curated reaction event. The source record connects ADP, Pi, SUCC-CoA to ATP, SUCCA, CoA-SH in mitochondrial matrix and cross-references RHEA:17663, RHEA:17662.", "equation": "ADP + Pi + SUCC-CoA → ATP + SUCCA + CoA-SH", "inputs": [ { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70958", "reactome_id": "R-ALL-70958", "label": "SUCC-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57292", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113536", "reactome_id": "R-ALL-113536", "label": "SUCCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30031", "formula": "C4H4O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "succinate-CoA ligase (ADP-forming) activity of SUCLA2:SUCLG1 [mitochondrial matrix]", "activity_id": "70996", "ec_numbers": [ "6.2.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:15877282", "title": "Deficiency of the ADP-forming succinyl-CoA synthase activity is associated with encephalomyopathy and mitochondrial DNA depletion", "journal": "Am J Hum Genet", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15877282/" }, { "id": "PMID:10727444", "title": "Interaction between succinyl CoA synthetase and the heme-biosynthetic enzyme ALAS-E is disrupted in sideroblastic anemia", "journal": "J Clin Invest", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10727444/" }, { "id": "PMID:9765291", "title": "Genetic evidence for the expression of ATP- and GTP-specific succinyl-CoA synthetases in multicellular eucaryotes", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9765291/" }, { "id": "PMID:9765290", "title": "Characterization of the ATP- and GTP-specific succinyl-CoA synthetases in pigeon. The enzymes incorporate the same alpha-subunit.", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9765290/" }, { "id": "PMID:15234968", "title": "Expression of two succinyl-CoA synthetases with different nucleotide specificities in mammalian tissues", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15234968/" }, { "id": "PMID:13181903", "title": "Guanosine triphosphate, the primary product of phosphorylation coupled to the breakdown of succinyl coenzyme A", "journal": "Biochim Biophys Acta", "year": 1954, "url": "https://pubmed.ncbi.nlm.nih.gov/13181903/" } ], "x": 5391.333333333333, "y": 335.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71010", "label": "MUT isomerises L-MM-CoA to SUCC-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:22889" ], "source_url": "https://reactome.org/content/detail/R-HSA-71010", "summary": "MUT isomerises L-MM-CoA to SUCC-CoA is a Reactome release 97 curated reaction event. The source record connects L-MM-CoA to SUCC-CoA in mitochondrial matrix and cross-references RHEA:22889.", "equation": "L-MM-CoA → SUCC-CoA", "inputs": [ { "node_id": "R-ALL-71009", "reactome_id": "R-ALL-71009", "label": "L-MM-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57326", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70958", "reactome_id": "R-ALL-70958", "label": "SUCC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57292", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methylmalonyl-CoA mutase activity of 2xMMAA:2xMUT:AdoCbl [mitochondrial matrix]", "activity_id": "71008", "ec_numbers": [ "5.4.99.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:2567699", "title": "Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction", "journal": "Genomics", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2567699/" }, { "id": "PMID:16281286", "title": "Spectrum of mutations in mut methylmalonic acidemia and identification of a common Hispanic mutation and haplotype", "journal": "Hum. Mutat.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16281286/" }, { "id": "PMID:20876572", "title": "Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20876572/" }, { "id": "PMID:21138732", "title": "Protection and reactivation of human methylmalonyl-CoA mutase by MMAA protein", "journal": "Biochem. Biophys. Res. Commun.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21138732/" }, { "id": "PMID:16866376", "title": "Assembly and protection of the radical enzyme, methylmalonyl-CoA mutase, by its chaperone", "journal": "Biochemistry", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16866376/" }, { "id": "PMID:12438653", "title": "Identification of the gene responsible for the cblA complementation group of vitamin B12-responsive methylmalonic acidemia based on analysis of prokaryotic gene arrangements", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12438653/" } ], "x": 868.6666666666667, "y": 1634.0877192982457, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71020", "label": "D-methylmalonyl-CoA L-methylmalonyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:20555" ], "source_url": "https://reactome.org/content/detail/R-HSA-71020", "summary": "D-methylmalonyl-CoA L-methylmalonyl-CoA is a Reactome release 97 curated reaction event. The source record connects MEMA-CoA to L-MM-CoA in mitochondrial matrix and cross-references RHEA:20555.", "equation": "MEMA-CoA → L-MM-CoA", "inputs": [ { "node_id": "R-ALL-71019", "reactome_id": "R-ALL-71019", "label": "MEMA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57327", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71009", "reactome_id": "R-ALL-71009", "label": "L-MM-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57326", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "methylmalonyl-CoA epimerase activity of MCEE [mitochondrial matrix]", "activity_id": "71018", "ec_numbers": [ "5.1.99.1" ], "accessions": [ "Q96PE7" ] } ], "citations": [ { "id": "PMID:13934211", "title": "Metabolism of propionic acid in animal tissues. IX. Methylmalonyl coenzyme A racemase", "journal": "J Biol Chem", "year": 1962, "url": "https://pubmed.ncbi.nlm.nih.gov/13934211/" }, { "id": "PMID:11481338", "title": "Identification of the human methylmalonyl-CoA racemase gene based on the analysis of prokaryotic gene arrangements. Implications for decoding the human genome.", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11481338/" } ], "x": 311.33333333333337, "y": 1637.1052631578948, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71031", "label": "propionyl-CoA + CO2 + ATP D-methylmalonyl-CoA + ADP + orthophosphate", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:23721" ], "source_url": "https://reactome.org/content/detail/R-HSA-71031", "summary": "propionyl-CoA + CO2 + ATP D-methylmalonyl-CoA + ADP + orthophosphate is a Reactome release 97 curated reaction event. The source record connects PROP-CoA, ATP, HCO3- to MEMA-CoA, ADP, Pi in mitochondrial matrix and cross-references RHEA:23721.", "equation": "PROP-CoA + ATP + HCO3- → MEMA-CoA + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-70843", "reactome_id": "R-ALL-70843", "label": "PROP-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57392", "formula": "C24H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29896", "reactome_id": "R-ALL-29896", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71019", "reactome_id": "R-ALL-71019", "label": "MEMA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57327", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "propionyl-CoA carboxylase activity of 6x(Btn-PCCA:PCCB) [mitochondrial matrix]", "activity_id": "71030", "ec_numbers": [ "6.4.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:6765947", "title": "Isolation and characterization of propionyl-CoA carboxylase from normal human liver. Evidence for a protomeric tetramer of nonidentical subunits", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6765947/" }, { "id": "PMID:13752080", "title": "Metabolism of propionic acid in animal tissues. VIII. Crystalline propionyl carboxylase", "journal": "J Biol Chem", "year": 1961, "url": "https://pubmed.ncbi.nlm.nih.gov/13752080/" }, { "id": "PMID:16023992", "title": "Mitochondrial targeting signals and mature peptides of 3-methylcrotonyl-CoA carboxylase", "journal": "Biochem Biophys Res Commun", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16023992/" } ], "x": 868.6666666666667, "y": 1655.2105263157894, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71046", "label": "glutaryl-CoA + FAD =\u003e crotonyl-CoA + FADH2 + CO2", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:30848" ], "source_url": "https://reactome.org/content/detail/R-HSA-71046", "summary": "glutaryl-CoA + FAD =\u003e crotonyl-CoA + FADH2 + CO2 is a Reactome release 97 curated reaction event. The source record connects FAD, GL-CoA, H+ to crotonyl-CoA, FADH2, CO2 in mitochondrial matrix and cross-references RHEA:30848.", "equation": "FAD + GL-CoA + 2 H+ → crotonyl-CoA + FADH2 + CO2", "inputs": [ { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70955", "reactome_id": "R-ALL-70955", "label": "GL-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57378", "formula": "C26H37N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-71045", "reactome_id": "R-ALL-71045", "label": "crotonyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57332", "formula": "C25H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutaryl-CoA dehydrogenase activity of GCDH tetramer [mitochondrial matrix]", "activity_id": "71044", "ec_numbers": [ "1.3.8.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:15274622", "title": "Crystal structures of human glutaryl-CoA dehydrogenase with and without an alternate substrate: structural bases of dehydrogenation and decarboxylation reactions", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15274622/" }, { "id": "PMID:18775954", "title": "Disease-causing missense mutations affect enzymatic activity, stability and oligomerization of glutaryl-CoA dehydrogenase (GCDH)", "journal": "Hum Mol Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18775954/" } ], "x": 6521.333333333333, "y": 2049.375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71118", "label": "PAH:Fe2+ tetramer hydroxylates L-Phe to L-Tyr", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20274" ], "source_url": "https://reactome.org/content/detail/R-HSA-71118", "summary": "PAH:Fe2+ tetramer hydroxylates L-Phe to L-Tyr is a Reactome release 97 curated reaction event. The source record connects L-Phe, BH4, O2 to L-Tyr, 4aOH-BH4 in cytosol and cross-references RHEA:20274.", "equation": "L-Phe + BH4 + O2 → L-Tyr + 4aOH-BH4", "inputs": [ { "node_id": "R-ALL-29502", "reactome_id": "R-ALL-29502", "label": "L-Phe", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58095", "formula": "C9H11NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29506", "reactome_id": "R-ALL-29506", "label": "L-Tyr", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58315", "formula": "C9H11NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71112", "reactome_id": "R-ALL-71112", "label": "4aOH-BH4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15642", "formula": "C9H15N5O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phenylalanine 4-monooxygenase activity of PAH:Fe2+ tetramer [cytosol]", "activity_id": "71074", "ec_numbers": [ "1.14.16.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12126628", "title": "Crystal structure of the ternary complex of the catalytic domain of human phenylalanine hydroxylase with tetrahydrobiopterin and 3-(2-thienyl)-L-alanine, and its implications for the mechanism of catalysis and substrate activation", "journal": "J Mol Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12126628/" }, { "id": "PMID:9642259", "title": "Structure of tetrameric human phenylalanine hydroxylase and its implications for phenylketonuria.", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9642259/" }, { "id": "PMID:8889590", "title": "Phenylalanine hydroxylase deficiency in a population in Germany: mutational profile and nine novel mutations", "journal": "Hum Mutat", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8889590/" } ], "x": 6800, "y": 1787.45, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71130", "label": "q-dihydrobiopterin + NADH + H+ =\u003e tetrahydrobiopterin + NAD+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:32253" ], "source_url": "https://reactome.org/content/detail/R-HSA-71130", "summary": "q-dihydrobiopterin + NADH + H+ =\u003e tetrahydrobiopterin + NAD+ is a Reactome release 97 curated reaction event. The source record connects qDHB, NADH, H+ to BH4, NAD+ in cytosol and cross-references RHEA:32253.", "equation": "qDHB + NADH + H+ → BH4 + NAD+", "inputs": [ { "node_id": "R-ALL-71129", "reactome_id": "R-ALL-71129", "label": "qDHB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43120", "formula": "C9H13N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29870", "reactome_id": "R-ALL-29870", "label": "BH4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59560", "formula": "C9H15N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "6,7-dihydropteridine reductase activity of QDPR dimer [cytosol]", "activity_id": "71128", "ec_numbers": [ "1.5.1.34" ], "accessions": [] } ], "citations": [ { "id": "PMID:3033643", "title": "Structure and expression of human dihydropteridine reductase.", "journal": "Proc Natl Acad Sci U S A", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3033643/" }, { "id": "PMID:8262916", "title": "The crystallographic structure of a human dihydropteridine reductase NADH binary complex expressed in Escherichia coli by a cDNA constructed from its rat homologue", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8262916/" } ], "x": 7078.666666666667, "y": 1787.45, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71146", "label": "4a-hydroxytetrahydrobiopterin =\u003e q-dihydrobiopterin + H2O", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11921" ], "source_url": "https://reactome.org/content/detail/R-HSA-71146", "summary": "4a-hydroxytetrahydrobiopterin =\u003e q-dihydrobiopterin + H2O is a Reactome release 97 curated reaction event. The source record connects 4aOH-BH4 to qDHB, H2O in cytosol and cross-references RHEA:11921.", "equation": "4aOH-BH4 → qDHB + H2O", "inputs": [ { "node_id": "R-ALL-71112", "reactome_id": "R-ALL-71112", "label": "4aOH-BH4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15642", "formula": "C9H15N5O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71129", "reactome_id": "R-ALL-71129", "label": "qDHB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43120", "formula": "C9H13N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "4-alpha-hydroxytetrahydrobiopterin dehydratase activity of PCBD1 tetramer [cytosol]", "activity_id": "71145", "ec_numbers": [ "4.2.1.96" ], "accessions": [] } ], "citations": [ { "id": "PMID:8352282", "title": "Mutation in the 4a-carbinolamine dehydratase gene leads to mild hyperphenylalaninemia with defective cofactor metabolism.", "journal": "Am J Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8352282/" }, { "id": "PMID:7744010", "title": "Three-dimensional structure of the bifunctional protein PCD/DCoH, a cytoplasmic enzyme interacting with transcription factor HNF1.", "journal": "EMBO J", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7744010/" }, { "id": "PMID:8444860", "title": "Phenylalanine hydroxylase-stimulating protein/pterin-4 alpha-carbinolamine dehydratase from rat and human liver. Purification, characterization, and complete amino acid sequence.", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8444860/" } ], "x": 6521.333333333333, "y": 1877.15, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71155", "label": "TAT deaminates tyrosine", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15094" ], "source_url": "https://reactome.org/content/detail/R-HSA-71155", "summary": "TAT deaminates tyrosine is a Reactome release 97 curated reaction event. The source record connects L-Tyr, 2OG to HPP, L-Glu in cytosol and cross-references RHEA:15094.", "equation": "L-Tyr + 2OG → HPP + L-Glu", "inputs": [ { "node_id": "R-ALL-29506", "reactome_id": "R-ALL-29506", "label": "L-Tyr", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58315", "formula": "C9H11NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-31365", "reactome_id": "R-ALL-31365", "label": "HPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36242", "formula": "C9H7O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-tyrosine:2-oxoglutarate transaminase activity of TAT dimer [cytosol]", "activity_id": "71154", "ec_numbers": [ "2.6.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:21153519", "title": "Tyrosine aminotransferase: biochemical and structural properties and molecular dynamics simulations", "journal": "Protein Cell", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21153519/" }, { "id": "PMID:16640556", "title": "The narrow substrate specificity of human tyrosine aminotransferase--the enzyme deficient in tyrosinemia type II", "journal": "FEBS J", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16640556/" }, { "id": "PMID:16602095", "title": "The genetic tyrosinemias", "journal": "Am J Med Genet C Semin Med Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16602095/" } ], "x": 7078.666666666667, "y": 1817.35, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71163", "label": "HPD dioxygenates HPP", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16190" ], "source_url": "https://reactome.org/content/detail/R-HSA-71163", "summary": "HPD dioxygenates HPP is a Reactome release 97 curated reaction event. The source record connects HPP, O2 to HGTA, CO2 in cytosol and cross-references RHEA:16190.", "equation": "HPP + O2 → HGTA + CO2", "inputs": [ { "node_id": "R-ALL-31365", "reactome_id": "R-ALL-31365", "label": "HPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36242", "formula": "C9H7O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30337", "reactome_id": "R-ALL-30337", "label": "HGTA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16169", "formula": "C8H7O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "4-hydroxyphenylpyruvate dioxygenase activity of HPD:AscH-:Fe2+ dimer [cytosol]", "activity_id": "71162", "ec_numbers": [ "1.13.11.27" ], "accessions": [] } ], "citations": [ { "id": "PMID:10942115", "title": "Mutations in the 4-hydroxyphenylpyruvate dioxygenase gene (HPD) in patients with tyrosinemia type III", "journal": "Hum Genet", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10942115/" }, { "id": "PMID:5487549", "title": "The mechanism of enzymic formation of homogentisate from p-hydroxyphenylpyruvate", "journal": "J. Am. Chem. Soc.", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/5487549/" }, { "id": "PMID:16602095", "title": "The genetic tyrosinemias", "journal": "Am J Med Genet C Semin Med Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16602095/" }, { "id": "PMID:37794595", "title": "Structure-based discovery of pyrazole-benzothiadiazole hybrid as human HPPD inhibitors", "journal": "Structure", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37794595/" }, { "id": "PMID:32750420", "title": "Identification of key residues determining the binding specificity of human 4-hydroxyphenylpyruvate dioxygenase", "journal": "Eur J Pharm Sci", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32750420/" }, { "id": "PMID:34047349", "title": "Functional role of residues involved in substrate binding of human 4-hydroxyphenylpyruvate dioxygenase", "journal": "Biochem J", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34047349/" } ], "x": 6521.333333333333, "y": 1847.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71164", "label": "HGD dioxygenates homogentisate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15450" ], "source_url": "https://reactome.org/content/detail/R-HSA-71164", "summary": "HGD dioxygenates homogentisate is a Reactome release 97 curated reaction event. The source record connects HGTA, O2 to 4MAA, H+ in cytosol and cross-references RHEA:15450.", "equation": "HGTA + O2 → 4MAA + H+", "inputs": [ { "node_id": "R-ALL-30337", "reactome_id": "R-ALL-30337", "label": "HGTA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16169", "formula": "C8H7O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-31135", "reactome_id": "R-ALL-31135", "label": "4MAA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17105", "formula": "C8H6O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "homogentisate 1,2-dioxygenase activity of HGD hexamer [cytosol]", "activity_id": "71084", "ec_numbers": [ "1.13.11.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:10876237", "title": "Crystal structure of human homogentisate dioxygenase.", "journal": "Nat Struct Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10876237/" }, { "id": "PMID:8782815", "title": "The molecular basis of alkaptonuria", "journal": "Nat Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8782815/" }, { "id": "PMID:32158253", "title": "Alkaptonuria: Current Perspectives", "journal": "Appl Clin Genet", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32158253/" }, { "id": "PMID:15479158", "title": "Steady-state kinetics and inhibition of anaerobically purified human homogentisate 1,2-dioxygenase", "journal": "Biochem J", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15479158/" } ], "x": 6800, "y": 1847.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71173", "label": "GSTZ1 isomerizes 4-MAA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14818" ], "source_url": "https://reactome.org/content/detail/R-HSA-71173", "summary": "GSTZ1 isomerizes 4-MAA is a Reactome release 97 curated reaction event. The source record connects 4MAA to 4FAA in cytosol and cross-references RHEA:14818.", "equation": "4MAA → 4FAA", "inputs": [ { "node_id": "R-ALL-31135", "reactome_id": "R-ALL-31135", "label": "4MAA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17105", "formula": "C8H6O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-31175", "reactome_id": "R-ALL-31175", "label": "4FAA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18034", "formula": "C8H6O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "maleylacetoacetate isomerase activity of GSTZ1 dimer [cytosol]", "activity_id": "71172", "ec_numbers": [ "5.2.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11327815", "title": "Crystal structure of maleylacetoacetate isomerase/glutathione transferase zeta reveals the molecular basis for its remarkable catalytic promiscuity.", "journal": "Biochemistry", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11327815/" }, { "id": "PMID:12852784", "title": "Clarification of the role of key active site residues of glutathione transferase zeta/maleylacetoacetate isomerase by a new spectrophotometric technique", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12852784/" }, { "id": "PMID:24637752", "title": "Preliminary X-ray crystallographic analysis of glutathione transferase zeta 1 (GSTZ1a-1a)", "journal": "Acta Crystallogr F Struct Biol Commun", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24637752/" } ], "x": 6800, "y": 1877.15, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71181", "label": "FAH cleaves 4FAA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10245" ], "source_url": "https://reactome.org/content/detail/R-HSA-71181", "summary": "FAH cleaves 4FAA is a Reactome release 97 curated reaction event. The source record connects 4FAA, H2O to FUMA, ACA, H+ in cytosol and cross-references RHEA:10245.", "equation": "4FAA + H2O → FUMA + ACA + H+", "inputs": [ { "node_id": "R-ALL-31175", "reactome_id": "R-ALL-31175", "label": "4FAA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18034", "formula": "C8H6O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29586", "reactome_id": "R-ALL-29586", "label": "FUMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29806", "formula": "C4H2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29666", "reactome_id": "R-ALL-29666", "label": "ACA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13705", "formula": "C4H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fumarylacetoacetase activity of FAH dimer [cytosol]", "activity_id": "71180", "ec_numbers": [ "3.7.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8364576", "title": "Characterization of the human fumarylacetoacetate hydrolase gene and identification of a missense mutation abolishing enzymatic activity.", "journal": "Hum Mol Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8364576/" }, { "id": "PMID:5027618", "title": "Purification and properties of a diketo acid hydrolase from beef liver", "journal": "Biochemistry", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/5027618/" }, { "id": "PMID:11127930", "title": "Hereditary tyrosinaemia type I: from basics to progress in treatment", "journal": "Ann Med", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11127930/" }, { "id": "PMID:16602095", "title": "The genetic tyrosinemias", "journal": "Am J Med Genet C Semin Med Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16602095/" } ], "x": 6521.333333333333, "y": 1817.35, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71188", "label": "TDO tetramer dioxygenates L-Trp to NFK", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24537" ], "source_url": "https://reactome.org/content/detail/R-HSA-71188", "summary": "TDO tetramer dioxygenates L-Trp to NFK is a Reactome release 97 curated reaction event. The source record connects L-Trp, O2 to NFK in cytosol and cross-references RHEA:24537.", "equation": "L-Trp + O2 → NFK", "inputs": [ { "node_id": "R-ALL-29500", "reactome_id": "R-ALL-29500", "label": "L-Trp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57912", "formula": "C11H12N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-33179", "reactome_id": "R-ALL-33179", "label": "NFK", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58629", "formula": "C11H12N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-tryptophan 2,3-dioxygenase activity of TDO2 tetramer [cytosol]", "activity_id": "71187", "ec_numbers": [ "1.13.11.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:8666386", "title": "Sequence of human tryptophan 2,3-dioxygenase (TDO2): presence of a glucocorticoid response-like element composed of a GTT repeat and an intronic CCCCT repeat", "journal": "Genomics", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8666386/" } ], "x": 7461.333333333333, "y": 1859.7083333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71189", "label": "AFMID hydrolyses NFK to L-KYN", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13010" ], "source_url": "https://reactome.org/content/detail/R-HSA-71189", "summary": "AFMID hydrolyses NFK to L-KYN is a Reactome release 97 curated reaction event. The source record connects NFK, H2O to L-KYN, HCOOH, H+ in cytosol and cross-references RHEA:13010.", "equation": "NFK + H2O → L-KYN + HCOOH + H+", "inputs": [ { "node_id": "R-ALL-33179", "reactome_id": "R-ALL-33179", "label": "NFK", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58629", "formula": "C11H12N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29970", "reactome_id": "R-ALL-29970", "label": "L-KYN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57959", "formula": "C10H12N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "arylformamidase activity of AFMID [cytosol]", "activity_id": "178109", "ec_numbers": [ "3.5.1.9" ], "accessions": [ "Q63HM1" ] } ], "citations": [ { "id": "PMID:12007602", "title": "Kynurenine formamidase: determination of primary structure and modeling-based prediction of tertiary structure and catalytic triad.", "journal": "Biochim Biophys Acta", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12007602/" } ], "x": 7461.333333333333, "y": 1809.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71200", "label": "kynurenine + O2 + NADPH + H+ =\u003e 3-hydroxykynurenine + NADP+ + H2O", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20546" ], "source_url": "https://reactome.org/content/detail/R-HSA-71200", "summary": "kynurenine + O2 + NADPH + H+ =\u003e 3-hydroxykynurenine + NADP+ + H2O is a Reactome release 97 curated reaction event. The source record connects L-KYN, O2, NADPH to 3HKYN, NADP+, H2O in cytosol and cross-references RHEA:20546.", "equation": "L-KYN + O2 + NADPH + H+ → 3HKYN + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-29970", "reactome_id": "R-ALL-29970", "label": "L-KYN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57959", "formula": "C10H12N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-34351", "reactome_id": "R-ALL-34351", "label": "3HKYN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58125", "formula": "C10H12N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "kynurenine 3-monooxygenase activity of KMO [mitochondrial outer membrane]", "activity_id": "71199", "ec_numbers": [ "1.14.13.9" ], "accessions": [ "O15229" ] } ], "citations": [ { "id": "PMID:10672018", "title": "Functional characterization and mechanism of action of recombinant human kynurenine 3-hydroxylase", "journal": "Eur J Biochem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10672018/" }, { "id": "PMID:4291901", "title": "On the submitochondrial localization of l-kynurenine-3-hydroxylase", "journal": "Biochem Biophys Res Commun", "year": 1967, "url": "https://pubmed.ncbi.nlm.nih.gov/4291901/" } ], "x": 7740, "y": 1784.9583333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71217", "label": "3-hydroxykynurenine + H2O =\u003e 3-hydroxyanthranilate + alanine + H+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25144" ], "source_url": "https://reactome.org/content/detail/R-HSA-71217", "summary": "3-hydroxykynurenine + H2O =\u003e 3-hydroxyanthranilate + alanine + H+ is a Reactome release 97 curated reaction event. The source record connects 3HKYN, H2O to 3HAA, L-Ala, H+ in cytosol and cross-references RHEA:25144.", "equation": "3HKYN + H2O → 3HAA + L-Ala + H+", "inputs": [ { "node_id": "R-ALL-34351", "reactome_id": "R-ALL-34351", "label": "3HKYN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58125", "formula": "C10H12N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30487", "reactome_id": "R-ALL-30487", "label": "3HAA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36559", "formula": "C7H6NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29432", "reactome_id": "R-ALL-29432", "label": "L-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "kynureninase activity of KYNU dimer [cytosol]", "activity_id": "71216", "ec_numbers": [ "3.7.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:9180257", "title": "Cloning and recombinant expression of rat and human kynureninase.", "journal": "FEBS Lett", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9180257/" } ], "x": 7740, "y": 1809.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71218", "label": "3-hydroxyanthranilate + O2 =\u003e 2-amino-3-carboxymuconate semialdehyde", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17954" ], "source_url": "https://reactome.org/content/detail/R-HSA-71218", "summary": "3-hydroxyanthranilate + O2 =\u003e 2-amino-3-carboxymuconate semialdehyde is a Reactome release 97 curated reaction event. The source record connects 3HAA, O2 to ACS in cytosol and cross-references RHEA:17954.", "equation": "3HAA + O2 → ACS", "inputs": [ { "node_id": "R-ALL-30487", "reactome_id": "R-ALL-30487", "label": "3HAA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36559", "formula": "C7H6NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-36027", "reactome_id": "R-ALL-36027", "label": "ACS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77612", "formula": "C7H6NO5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-hydroxyanthranilate 3,4-dioxygenase activity of HAAO [cytosol]", "activity_id": "71096", "ec_numbers": [ "1.13.11.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:7514594", "title": "Molecular cloning and functional expression of human 3-hydroxyanthranilic-acid dioxygenase.", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7514594/" } ], "x": 7740, "y": 1859.7083333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71223", "label": "2-amino-3-carboxymuconate semialdehyde =\u003e 2-aminomuconate semialdehyde + CO2", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16558" ], "source_url": "https://reactome.org/content/detail/R-HSA-71223", "summary": "2-amino-3-carboxymuconate semialdehyde =\u003e 2-aminomuconate semialdehyde + CO2 is a Reactome release 97 curated reaction event. The source record connects ACS, H+ to 2AMA, CO2 in cytosol and cross-references RHEA:16558.", "equation": "ACS + H+ → 2AMA + CO2", "inputs": [ { "node_id": "R-ALL-36027", "reactome_id": "R-ALL-36027", "label": "ACS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77612", "formula": "C7H6NO5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-35181", "reactome_id": "R-ALL-35181", "label": "2AMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77634", "formula": "C6H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carboxy-lyase activity of ACMSD dimer [cytosol]", "activity_id": "71222", "ec_numbers": [ "4.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12140278", "title": "Identification and expression of a cDNA encoding human alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase (ACMSD). A key enzyme for the tryptophan-niacine pathway and \"quinolinate hypothesis\".", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12140278/" } ], "x": 7461.333333333333, "y": 1909.5416666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71239", "label": "2-aminomuconate semialdehyde + NAD+ + H2O =\u003e aminomuconate + NADH + H+", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14470" ], "source_url": "https://reactome.org/content/detail/R-HSA-71239", "summary": "2-aminomuconate semialdehyde + NAD+ + H2O =\u003e aminomuconate + NADH + H+ is a Reactome release 97 curated reaction event. The source record connects 2AMA, NAD+, H2O to 2AM, NADH, H+ in cytosol and cross-references RHEA:14470.", "equation": "2AMA + NAD+ + H2O → 2AM + NADH + H+", "inputs": [ { "node_id": "R-ALL-35181", "reactome_id": "R-ALL-35181", "label": "2AMA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77634", "formula": "C6H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-32913", "reactome_id": "R-ALL-32913", "label": "2AM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77859", "formula": "C6H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aminomuconate-semialdehyde dehydrogenase activity of AM-SHDH [cytosol]", "activity_id": "71238", "ec_numbers": [ "1.2.1.32" ], "accessions": [] } ], "citations": [ { "id": "PMID:14275130", "title": "Studies on the metabolism of the benzene ring of tryptophan in mammalian tissues. II. Enzymatic formation of alpha-muconic acid from 2-hydroxyanthranilic acid", "journal": "J Biol Chem", "year": 1965, "url": "https://pubmed.ncbi.nlm.nih.gov/14275130/" } ], "x": 8018.666666666667, "y": 1784.9583333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71296", "label": "PGLS hydrolyzes D-glucono-1,5-lactone 6-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12557" ], "source_url": "https://reactome.org/content/detail/R-HSA-71296", "summary": "PGLS hydrolyzes D-glucono-1,5-lactone 6-phosphate is a Reactome release 97 curated reaction event. The source record connects D-Glucono-1,5-lactone 6-phosphate, H2O to PDG, H+ in cytosol and cross-references RHEA:12557.", "equation": "D-Glucono-1,5-lactone 6-phosphate + H2O → PDG + H+", "inputs": [ { "node_id": "R-ALL-31467", "reactome_id": "R-ALL-31467", "label": "D-Glucono-1,5-lactone 6-phosphate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57955", "formula": "C6H9O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29996", "reactome_id": "R-ALL-29996", "label": "PDG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58759", "formula": "C6H10O10P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "6-phosphogluconolactonase activity of PGLS [cytosol]", "activity_id": "71295", "ec_numbers": [ "3.1.1.31" ], "accessions": [ "O95336" ] } ], "citations": [ { "id": "PMID:3932573", "title": "Limiting role of 6-phosphogluconolactonase in erythrocyte hexose monophosphate pathway metabolism", "journal": "J Lab Clin Med", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3932573/" }, { "id": "PMID:10518023", "title": "Identification of the cDNA encoding human 6-phosphogluconolactonase, the enzyme catalyzing the second step of the pentose phosphate pathway(1)", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10518023/" } ], "x": 311.33333333333337, "y": 880.9285714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71299", "label": "PGD decarboxylates 6-phospho-D-gluconate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10117" ], "source_url": "https://reactome.org/content/detail/R-HSA-71299", "summary": "PGD decarboxylates 6-phospho-D-gluconate is a Reactome release 97 curated reaction event. The source record connects PDG, NADP+ to RU5P, CO2, NADPH in cytosol and cross-references RHEA:10117.", "equation": "PDG + NADP+ → RU5P + CO2 + NADPH", "inputs": [ { "node_id": "R-ALL-29996", "reactome_id": "R-ALL-29996", "label": "PDG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58759", "formula": "C6H10O10P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29732", "reactome_id": "R-ALL-29732", "label": "RU5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58121", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphogluconate dehydrogenase (decarboxylating) activity of PGD dimer [cytosol]", "activity_id": "71298", "ec_numbers": [ "1.1.1.44" ], "accessions": [] } ], "citations": [ { "id": "PMID:3932573", "title": "Limiting role of 6-phosphogluconolactonase in erythrocyte hexose monophosphate pathway metabolism", "journal": "J Lab Clin Med", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3932573/" }, { "id": "PMID:3994686", "title": "Half-site reactivity in 6-phosphogluconate dehydrogenase from human erythrocytes", "journal": "Biochem J", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/3994686/" }, { "id": "PMID:9920387", "title": "6-Phosphogluconate dehydrogenase: the mechanism of action investigated by a comparison of the enzyme from different species", "journal": "Biochim Biophys Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9920387/" } ], "x": 311.33333333333337, "y": 769.7857142857143, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71303", "label": "RPE dimers isomerise RU5P to XY5P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13678" ], "source_url": "https://reactome.org/content/detail/R-HSA-71303", "summary": "RPE dimers isomerise RU5P to XY5P is a Reactome release 97 curated reaction event. The source record connects RU5P to XY5P in cytosol and cross-references RHEA:13678.", "equation": "RU5P → XY5P", "inputs": [ { "node_id": "R-ALL-29732", "reactome_id": "R-ALL-29732", "label": "RU5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58121", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29790", "reactome_id": "R-ALL-29790", "label": "XY5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57737", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "D-ribulose-phosphate 3-epimerase activity of RPE:Fe2+ dimers [cytosol]", "activity_id": "71302", "ec_numbers": [ "5.1.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2581946", "title": "Phosphoribosylpyrophosphate synthesis from glucose decreases during amino acid starvation of human lymphoblasts", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2581946/" }, { "id": "PMID:7396409", "title": "Biochemical genetics of the pentose phosphate cycle: human ribose 5-phosphate isomerase (RPI) and ribulose 5-phosphate 3-epimerase (RPE)", "journal": "Ann Hum Genet", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/7396409/" }, { "id": "PMID:20923965", "title": "Conversion of D-ribulose 5-phosphate to D-xylulose 5-phosphate: new insights from structural and biochemical studies on human RPE", "journal": "FASEB J.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/20923965/" } ], "x": 311.33333333333337, "y": 992.0714285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71306", "label": "RPIA isomerizes D-ribulose 5-phosphate to ribose 5-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14659" ], "source_url": "https://reactome.org/content/detail/R-HSA-71306", "summary": "RPIA isomerizes D-ribulose 5-phosphate to ribose 5-phosphate is a Reactome release 97 curated reaction event. The source record connects RU5P to R5P in cytosol and cross-references RHEA:14659.", "equation": "RU5P → R5P", "inputs": [ { "node_id": "R-ALL-29732", "reactome_id": "R-ALL-29732", "label": "RU5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58121", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ribose-5-phosphate isomerase activity of RPIA [cytosol]", "activity_id": "71305", "ec_numbers": [ "5.3.1.6" ], "accessions": [ "P49247" ] } ], "citations": [ { "id": "PMID:14988808", "title": "Ribose-5-phosphate isomerase deficiency: new inborn error in the pentose phosphate pathway associated with a slowly progressive leukoencephalopathy", "journal": "Am J Hum Genet", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14988808/" } ], "x": 311.33333333333337, "y": 1103.2142857142858, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71324", "label": "ribose 5-phosphate + xylulose 5-phosphate sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10510" ], "source_url": "https://reactome.org/content/detail/R-HSA-71324", "summary": "ribose 5-phosphate + xylulose 5-phosphate sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate is a Reactome release 97 curated reaction event. The source record connects XY5P, R5P to GA3P, SH7P in cytosol and cross-references RHEA:10510.", "equation": "XY5P + R5P → GA3P + SH7P", "inputs": [ { "node_id": "R-ALL-29790", "reactome_id": "R-ALL-29790", "label": "XY5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57737", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29882", "reactome_id": "R-ALL-29882", "label": "SH7P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57483", "formula": "C7H13O10P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transketolase activity of TKT dimer [cytosol]", "activity_id": "71323", "ec_numbers": [ "2.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9357955", "title": "Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation", "journal": "Biochim Biophys Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9357955/" } ], "x": 590, "y": 1047.642857142857, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71334", "label": "sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate D-erythrose 4-phosphate + D-fructose 6-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17054" ], "source_url": "https://reactome.org/content/detail/R-HSA-71334", "summary": "sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate D-erythrose 4-phosphate + D-fructose 6-phosphate is a Reactome release 97 curated reaction event. The source record connects SH7P, GA3P to Fru(6)P, E4P in cytosol and cross-references RHEA:17054.", "equation": "SH7P + GA3P → Fru(6)P + E4P", "inputs": [ { "node_id": "R-ALL-29882", "reactome_id": "R-ALL-29882", "label": "SH7P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57483", "formula": "C7H13O10P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29878", "reactome_id": "R-ALL-29878", "label": "E4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16897", "formula": "C4H7O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transaldolase activity of TALDO1 dimer [cytosol]", "activity_id": "71333", "ec_numbers": [ "2.2.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8300619", "title": "Cloning and expression of the human gene for transaldolase. A novel highly repetitive element constitutes an integral part of the coding sequence", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8300619/" }, { "id": "PMID:10869557", "title": "The three-dimensional structure of human transaldolase", "journal": "FEBS Lett.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10869557/" }, { "id": "PMID:11283793", "title": "Transaldolase deficiency: liver cirrhosis associated with a new inborn error in the pentose phosphate pathway.", "journal": "Am J Hum Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11283793/" } ], "x": 590, "y": 880.9285714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71335", "label": "xylulose 5-phosphate + D-erythrose 4-phosphate D-glyceraldehyde 3-phosphate + D-fructose 6-phosphate", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27627" ], "source_url": "https://reactome.org/content/detail/R-HSA-71335", "summary": "xylulose 5-phosphate + D-erythrose 4-phosphate D-glyceraldehyde 3-phosphate + D-fructose 6-phosphate is a Reactome release 97 curated reaction event. The source record connects XY5P, E4P to GA3P, Fru(6)P in cytosol and cross-references RHEA:27627.", "equation": "XY5P + E4P → GA3P + Fru(6)P", "inputs": [ { "node_id": "R-ALL-29790", "reactome_id": "R-ALL-29790", "label": "XY5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57737", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29878", "reactome_id": "R-ALL-29878", "label": "E4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16897", "formula": "C4H7O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "transketolase activity of TKT dimer [cytosol]", "activity_id": "71323", "ec_numbers": [ "2.2.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9357955", "title": "Aspartate 155 of human transketolase is essential for thiamine diphosphate-magnesium binding, and cofactor binding is required for dimer formation", "journal": "Biochim Biophys Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9357955/" } ], "x": 868.6666666666667, "y": 880.9285714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71401", "label": "OGDH dimer decarboxylates 2-OG", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12189" ], "source_url": "https://reactome.org/content/detail/R-HSA-71401", "summary": "OGDH dimer decarboxylates 2-OG is a Reactome release 97 curated reaction event. The source record connects 2OG, H+, Lipo-K110-DLST to CO2, SuccinyllipoH2-K110-DLST in mitochondrial matrix and cross-references RHEA:12189.", "equation": "2OG + H+ + Lipo-K110-DLST → CO2 + SuccinyllipoH2-K110-DLST", "inputs": [ { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-69993", "reactome_id": "R-HSA-69993", "label": "Lipo-K110-DLST", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9853502", "reactome_id": "R-HSA-9853502", "label": "SuccinyllipoH2-K110-DLST", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "oxoglutarate dehydrogenase (succinyl-transferring) activity of OGDH complex [mitochondrial matrix]", "activity_id": "9859269", "ec_numbers": [ "1.2.4.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:35272141", "title": "Structural basis for the activity and regulation of human α-ketoglutarate dehydrogenase revealed by Cryo-EM", "journal": "Biochem Biophys Res Commun", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35272141/" }, { "id": "PMID:28435050", "title": "The human Krebs cycle 2-oxoglutarate dehydrogenase complex creates an additional source of superoxide/hydrogen peroxide from 2-oxoadipate as alternative substrate", "journal": "Free Radic Biol Med", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28435050/" }, { "id": "PMID:25210035", "title": "Human 2-oxoglutarate dehydrogenase complex E1 component forms a thiamin-derived radical by aerobic oxidation of the enamine intermediate", "journal": "J Biol Chem", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25210035/" }, { "id": "PMID:24495017", "title": "Studies on the regulation of the human E1 subunit of the 2-oxoglutarate dehydrogenase complex, including the identification of a novel calcium-binding site", "journal": "Biochem J", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24495017/" } ], "x": 5948.666666666667, "y": 433.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71445", "label": "PGAM dimers isomerise 2PG to 3PG", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15902" ], "source_url": "https://reactome.org/content/detail/R-HSA-71445", "summary": "PGAM dimers isomerise 2PG to 3PG is a Reactome release 97 curated reaction event. The source record connects 2PG to 3PG in cytosol and cross-references RHEA:15902.", "equation": "2PG → 3PG", "inputs": [ { "node_id": "R-ALL-30485", "reactome_id": "R-ALL-30485", "label": "2PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58289", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29728", "reactome_id": "R-ALL-29728", "label": "3PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58272", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphoglycerate mutase activity of PGAM dimers [cytosol]", "activity_id": "71444", "ec_numbers": [ "5.4.2.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:4827367", "title": "Phosphoglycerate mutase isozyme marker for tissue differentiation in man", "journal": "Am J Hum Genet", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4827367/" }, { "id": "PMID:15710582", "title": "Phosphoglycerate mutase BB isoenzyme deficiency in a patient with non-spherocytic anemia: familial and metabolic studies", "journal": "Haematologica", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15710582/" }, { "id": "PMID:8447317", "title": "The molecular genetic basis of muscle phosphoglycerate mutase (PGAM) deficiency", "journal": "Am J Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8447317/" } ], "x": 1530, "y": 489.91666666666663, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71495", "label": "Aldolase tetramers convert GA3P and DHAP to F1,6PP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14731" ], "source_url": "https://reactome.org/content/detail/R-HSA-71495", "summary": "Aldolase tetramers convert GA3P and DHAP to F1,6PP is a Reactome release 97 curated reaction event. The source record connects DHAP, GA3P to F1,6PP in cytosol and cross-references RHEA:14731.", "equation": "DHAP + GA3P → F1,6PP", "inputs": [ { "node_id": "R-ALL-75970", "reactome_id": "R-ALL-75970", "label": "DHAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57642", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30014", "reactome_id": "R-ALL-30014", "label": "F1,6PP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32966", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fructose-bisphosphate aldolase activity of aldolase tetramer [cytosol]", "activity_id": "71494", "ec_numbers": [ "4.1.2.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:7717389", "title": "Diverse mutations in the aldolase B gene that underlie the prevalence of hereditary fructose intolerance", "journal": "Am J Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7717389/" }, { "id": "PMID:3355497", "title": "The complete amino acid sequence of human skeletal-muscle fructose-bisphosphate aldolase", "journal": "Biochem J", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3355497/" } ], "x": 1530, "y": 425.0833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71496", "label": "Aldolase tetramer cleaves F1,6PP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14730" ], "source_url": "https://reactome.org/content/detail/R-HSA-71496", "summary": "Aldolase tetramer cleaves F1,6PP is a Reactome release 97 curated reaction event. The source record connects F1,6PP to DHAP, GA3P in cytosol and cross-references RHEA:14730.", "equation": "F1,6PP → DHAP + GA3P", "inputs": [ { "node_id": "R-ALL-30014", "reactome_id": "R-ALL-30014", "label": "F1,6PP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32966", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-75970", "reactome_id": "R-ALL-75970", "label": "DHAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57642", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29578", "reactome_id": "R-ALL-29578", "label": "GA3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59776", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "fructose-bisphosphate aldolase activity of aldolase tetramer [cytosol]", "activity_id": "71494", "ec_numbers": [ "4.1.2.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:7717389", "title": "Diverse mutations in the aldolase B gene that underlie the prevalence of hereditary fructose intolerance", "journal": "Am J Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7717389/" }, { "id": "PMID:6696436", "title": "Human skeletal-muscle aldolase: N-terminal sequence analysis of CNBr- and o-iodosobenzoic acid-cleavage fragments", "journal": "Arch Biochem Biophys", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6696436/" }, { "id": "PMID:3355497", "title": "The complete amino acid sequence of human skeletal-muscle fructose-bisphosphate aldolase", "journal": "Biochem J", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3355497/" } ], "x": 311.33333333333337, "y": 433.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71515", "label": "glycogen-glycogenin-1 + n orthophosphate =\u003e limit dextrin-glycogenin-1 + n D-glucose 1-phosphate [PYGM,PYGB]", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:41733" ], "source_url": "https://reactome.org/content/detail/R-HSA-71515", "summary": "glycogen-glycogenin-1 + n orthophosphate =\u003e limit dextrin-glycogenin-1 + n D-glucose 1-phosphate [PYGM,PYGB] is a Reactome release 97 curated reaction event. The source record connects glycogen-glycogenin-1 dimer, Pi to limit dextrin-glycogenin-1 dimer, G1P in cytosol and cross-references RHEA:41733.", "equation": "glycogen-glycogenin-1 dimer + Pi → limit dextrin-glycogenin-1 dimer + G1P", "inputs": [ { "node_id": "R-HSA-453239", "reactome_id": "R-HSA-453239", "label": "glycogen-glycogenin-1 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-453345", "reactome_id": "R-HSA-453345", "label": "limit dextrin-glycogenin-1 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29548", "reactome_id": "R-ALL-29548", "label": "G1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58601", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycogen phosphorylase activity of active PYGM and PYGB dimers [cytosol]", "activity_id": "71514", "ec_numbers": [ "2.4.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10949035", "title": "Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core", "journal": "Mol Cell", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10949035/" }, { "id": "PMID:2667896", "title": "The family of glycogen phosphorylases: structure and function", "journal": "Crit Rev Biochem Mol Biol", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2667896/" } ], "x": 1251.3333333333333, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71541", "label": "glycogen phosphorylase (PYGM) dimer b + 2 ATP =\u003e glycogen phosphorylase (PYGM) dimer a + 2 ADP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17990" ], "source_url": "https://reactome.org/content/detail/R-HSA-71541", "summary": "glycogen phosphorylase (PYGM) dimer b + 2 ATP =\u003e glycogen phosphorylase (PYGM) dimer a + 2 ADP is a Reactome release 97 curated reaction event. The source record connects PYGM dimer, b form, ATP to PYGM dimer a form, ADP, H+ in cytosol and cross-references RHEA:17990.", "equation": "PYGM dimer, b form + 2 ATP → PYGM dimer a form + 2 ADP + 2 H+", "inputs": [ { "node_id": "R-HSA-71538", "reactome_id": "R-HSA-71538", "label": "PYGM dimer, b form", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-71513", "reactome_id": "R-HSA-71513", "label": "PYGM dimer a form", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphorylase kinase activity of phosphorylase kinase complex (PHKM) [cytosol]", "activity_id": "71521", "ec_numbers": [ "2.7.11.19" ], "accessions": [] } ], "citations": [ { "id": "PMID:10487978", "title": "Phosphorylase kinase: the complexity of its regulation is reflected in the complexity of its structure", "journal": "Front Biosci", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10487978/" }, { "id": "PMID:12825073", "title": "Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases", "journal": "Eur J Hum Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12825073/" }, { "id": "PMID:5461220", "title": "Correlation between subunit interactions and enzymatic activity of phosphorylase a. Method for determining equilibrium constants from initial rate measurements", "journal": "Biochemistry", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/5461220/" }, { "id": "PMID:13115432", "title": "Enzymic conversion of phosphorylase a to phosphorylase b", "journal": "Biochim Biophys Acta", "year": 1953, "url": "https://pubmed.ncbi.nlm.nih.gov/13115432/" } ], "x": 1251.3333333333333, "y": 876.6538461538462, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71552", "label": "limit dextrin-glycogenin =\u003e ((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl) glycogenin", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-71552", "summary": "limit dextrin-glycogenin =\u003e ((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl) glycogenin is a Reactome release 97 curated reaction event. The source record connects limit dextrin-glycogenin dimer to ((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl)GYG dimer in cytosol.", "equation": "limit dextrin-glycogenin dimer → ((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl)GYG dimer", "inputs": [ { "node_id": "R-HSA-453336", "reactome_id": "R-HSA-453336", "label": "limit dextrin-glycogenin dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-453350", "reactome_id": "R-HSA-453350", "label": "((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl)GYG dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "4-alpha-glucanotransferase activity of AGL [cytosol]", "activity_id": "71550", "ec_numbers": [ "2.4.1.25" ], "accessions": [ "P35573" ] } ], "citations": [ { "id": "PMID:2961257", "title": "Glycogen debranching enzyme: purification, antibody characterization, and immunoblot analyses of type III glycogen storage disease.", "journal": "Am J Hum Genet", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2961257/" }, { "id": "PMID:8755644", "title": "Mutations in exon 3 of the glycogen debranching enzyme gene are associated with glycogen storage disease type III that is differentially expressed in liver and muscle.", "journal": "J Clin Invest", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8755644/" } ], "x": 1251.3333333333333, "y": 1086.1153846153848, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71588", "label": "glycogen phosphorylase (PYGL) dimer b + 2 ATP =\u003e glycogen phosphorylase (PYGL) dimer a + 2 ADP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17990" ], "source_url": "https://reactome.org/content/detail/R-HSA-71588", "summary": "glycogen phosphorylase (PYGL) dimer b + 2 ATP =\u003e glycogen phosphorylase (PYGL) dimer a + 2 ADP is a Reactome release 97 curated reaction event. The source record connects PYGL dimer b form, ATP to PYGL dimer a form, ADP, H+ in cytosol and cross-references RHEA:17990.", "equation": "PYGL dimer b form + 2 ATP → PYGL dimer a form + 2 ADP + 2 H+", "inputs": [ { "node_id": "R-HSA-71581", "reactome_id": "R-HSA-71581", "label": "PYGL dimer b form", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-71586", "reactome_id": "R-HSA-71586", "label": "PYGL dimer a form", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphorylase kinase activity of phosphorylase kinase complex (PHKL) [cytosol]", "activity_id": "71535", "ec_numbers": [ "2.7.11.19" ], "accessions": [] } ], "citations": [ { "id": "PMID:10487978", "title": "Phosphorylase kinase: the complexity of its regulation is reflected in the complexity of its structure", "journal": "Front Biosci", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10487978/" }, { "id": "PMID:12825073", "title": "Muscle glycogenosis with low phosphorylase kinase activity: mutations in PHKA1, PHKG1 or six other candidate genes explain only a minority of cases", "journal": "Eur J Hum Genet", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12825073/" }, { "id": "PMID:5461220", "title": "Correlation between subunit interactions and enzymatic activity of phosphorylase a. Method for determining equilibrium constants from initial rate measurements", "journal": "Biochemistry", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/5461220/" }, { "id": "PMID:13115432", "title": "Enzymic conversion of phosphorylase a to phosphorylase b", "journal": "Biochim Biophys Acta", "year": 1953, "url": "https://pubmed.ncbi.nlm.nih.gov/13115432/" }, { "id": "PMID:12930917", "title": "Severe phenotype of phosphorylase kinase-deficient liver glycogenosis with mutations in the PHKG2 gene", "journal": "Pediatr Res", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12930917/" }, { "id": "PMID:7847371", "title": "X-linked liver phosphorylase kinase deficiency is associated with mutations in the human liver phosphorylase kinase alpha subunit.", "journal": "Am J Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7847371/" } ], "x": 1530, "y": 876.6538461538462, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71590", "label": "glycogen-glycogenin-2 + n orthophosphate =\u003e limit dextrin-glycogenin-2 + n D-glucose 1-phosphate [PYGL]", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:41733" ], "source_url": "https://reactome.org/content/detail/R-HSA-71590", "summary": "glycogen-glycogenin-2 + n orthophosphate =\u003e limit dextrin-glycogenin-2 + n D-glucose 1-phosphate [PYGL] is a Reactome release 97 curated reaction event. The source record connects glycogen-glycogenin-2 dimer, Pi to limit dextrin-glycogenin-2 dimer, G1P in cytosol and cross-references RHEA:41733.", "equation": "glycogen-glycogenin-2 dimer + Pi → limit dextrin-glycogenin-2 dimer + G1P", "inputs": [ { "node_id": "R-HSA-453240", "reactome_id": "R-HSA-453240", "label": "glycogen-glycogenin-2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-453330", "reactome_id": "R-HSA-453330", "label": "limit dextrin-glycogenin-2 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29548", "reactome_id": "R-ALL-29548", "label": "G1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58601", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycogen phosphorylase activity of PYGL dimer a form [cytosol]", "activity_id": "71587", "ec_numbers": [ "2.4.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10949035", "title": "Activation of human liver glycogen phosphorylase by alteration of the secondary structure and packing of the catalytic core", "journal": "Mol Cell", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10949035/" }, { "id": "PMID:2667896", "title": "The family of glycogen phosphorylases: structure and function", "journal": "Crit Rev Biochem Mol Biol", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2667896/" } ], "x": 1530, "y": 936.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71593", "label": "((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl)glycogenin =\u003e poly{(1,4)-alpha-glucosyl} glycogenin + alpha-D-glucose", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-71593", "summary": "((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl)glycogenin =\u003e poly{(1,4)-alpha-glucosyl} glycogenin + alpha-D-glucose is a Reactome release 97 curated reaction event. The source record connects ((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl)GYG dimer to poly((1,4)-alpha-glucosyl)GYG dimer, Glc in cytosol.", "equation": "((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl)GYG dimer → poly((1,4)-alpha-glucosyl)GYG dimer + Glc", "inputs": [ { "node_id": "R-HSA-453350", "reactome_id": "R-HSA-453350", "label": "((1,6)-alpha-glucosyl)poly((1,4)-alpha-glucosyl)GYG dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-453224", "reactome_id": "R-HSA-453224", "label": "poly((1,4)-alpha-glucosyl)GYG dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-70113", "reactome_id": "R-ALL-70113", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "amylo-alpha-1,6-glucosidase activity of AGL [cytosol]", "activity_id": "71551", "ec_numbers": [ "3.2.1.33" ], "accessions": [ "P35573" ] } ], "citations": [ { "id": "PMID:2961257", "title": "Glycogen debranching enzyme: purification, antibody characterization, and immunoblot analyses of type III glycogen storage disease.", "journal": "Am J Hum Genet", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2961257/" }, { "id": "PMID:9691087", "title": "Mutations in the liver glycogen synthase gene in children with hypoglycemia due to glycogen storage disease type 0", "journal": "J Clin Invest", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9691087/" } ], "x": 1530, "y": 996.3461538461538, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71654", "label": "PGAM dimers isomerise 3PG to 2PG", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15903" ], "source_url": "https://reactome.org/content/detail/R-HSA-71654", "summary": "PGAM dimers isomerise 3PG to 2PG is a Reactome release 97 curated reaction event. The source record connects 3PG to 2PG in cytosol and cross-references RHEA:15903.", "equation": "3PG → 2PG", "inputs": [ { "node_id": "R-ALL-29728", "reactome_id": "R-ALL-29728", "label": "3PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58272", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30485", "reactome_id": "R-ALL-30485", "label": "2PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58289", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphoglycerate mutase activity of PGAM dimers [cytosol]", "activity_id": "71444", "ec_numbers": [ "5.4.2.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:2846554", "title": "Sequence of the human erythrocyte phosphoglycerate mutase by microsequencer and mass spectrometry", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2846554/" }, { "id": "PMID:4827367", "title": "Phosphoglycerate mutase isozyme marker for tissue differentiation in man", "journal": "Am J Hum Genet", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4827367/" }, { "id": "PMID:15710582", "title": "Phosphoglycerate mutase BB isoenzyme deficiency in a patient with non-spherocytic anemia: familial and metabolic studies", "journal": "Haematologica", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15710582/" }, { "id": "PMID:8447317", "title": "The molecular genetic basis of muscle phosphoglycerate mutase (PGAM) deficiency", "journal": "Am J Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8447317/" } ], "x": 590, "y": 481.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71660", "label": "Enolase dimers dehydrates 2PG", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10165" ], "source_url": "https://reactome.org/content/detail/R-HSA-71660", "summary": "Enolase dimers dehydrates 2PG is a Reactome release 97 curated reaction event. The source record connects 2PG to PEP, H2O in cytosol and cross-references RHEA:10165.", "equation": "2PG → PEP + H2O", "inputs": [ { "node_id": "R-ALL-30485", "reactome_id": "R-ALL-30485", "label": "2PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58289", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29492", "reactome_id": "R-ALL-29492", "label": "PEP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58702", "formula": "C3H2O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphopyruvate hydratase activity of enolase dimer [cytosol]", "activity_id": "71439", "ec_numbers": [ "4.2.1.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:11506403", "title": "Beta-enolase deficiency, a new metabolic myopathy of distal glycolysis", "journal": "Ann Neurol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11506403/" }, { "id": "PMID:3529090", "title": "Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3529090/" }, { "id": "PMID:3208766", "title": "Complete amino acid sequence of the neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE)", "journal": "Eur J Biochem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3208766/" }, { "id": "PMID:23446454", "title": "Disruption of a spermatogenic cell-specific mouse enolase 4 (eno4) gene causes sperm structural defects and male infertility", "journal": "Biol Reprod", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23446454/" }, { "id": "PMID:4413246", "title": "Enolase isoenzymes in rat tissues. Electrophoretic, chromatographic, immunological and kinetic properties", "journal": "Biochim Biophys Acta", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4413246/" }, { "id": "PMID:2462567", "title": "Tau-crystallin/alpha-enolase: one gene encodes both an enzyme and a lens structural protein", "journal": "J Cell Biol", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2462567/" } ], "x": 590, "y": 433.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71670", "label": "Pyruvate kinase dephosphorylates PEP to PYR", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis", "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18159" ], "source_url": "https://reactome.org/content/detail/R-HSA-71670", "summary": "Pyruvate kinase dephosphorylates PEP to PYR is a Reactome release 97 curated reaction event. The source record connects ADP, PEP, H+ to ATP, PYR in cytosol and cross-references RHEA:18159.", "equation": "ADP + PEP + H+ → ATP + PYR", "inputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29492", "reactome_id": "R-ALL-29492", "label": "PEP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58702", "formula": "C3H2O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pyruvate kinase activity of pyruvate kinase tetramer [cytosol]", "activity_id": "71669", "ec_numbers": [ "2.7.1.40" ], "accessions": [] } ], "citations": [ { "id": "PMID:15996096", "title": "Structural basis for tumor pyruvate kinase M2 allosteric regulation and catalysis", "journal": "Biochemistry", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15996096/" }, { "id": "PMID:1896471", "title": "cDNA cloning of human R-type pyruvate kinase and identification of a single amino acid substitution (Thr384----Met) affecting enzymatic stability in a pyruvate kinase variant (PK Tokyo) associated with hereditary hemolytic anemia", "journal": "Proc Natl Acad Sci U S A", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1896471/" }, { "id": "PMID:18191611", "title": "Pyruvate kinase isozyme type M2 (PKM2) interacts and cooperates with Oct-4 in regulating transcription", "journal": "Int J Biochem Cell Biol", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18191611/" }, { "id": "PMID:2040271", "title": "Isolation and characterization of the human pyruvate kinase M gene", "journal": "Eur J Biochem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2040271/" }, { "id": "PMID:3126495", "title": "Human liver type pyruvate kinase: complete amino acid sequence and the expression in mammalian cells", "journal": "Proc Natl Acad Sci U S A", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3126495/" }, { "id": "PMID:11960989", "title": "Structure and function of human erythrocyte pyruvate kinase. Molecular basis of nonspherocytic hemolytic anemia", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11960989/" } ], "x": 868.6666666666667, "y": 287.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71682", "label": "glutathione (oxidized) + NADPH + H+ =\u003e 2 glutathione (reduced) + NADP+", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11742" ], "source_url": "https://reactome.org/content/detail/R-HSA-71682", "summary": "glutathione (oxidized) + NADPH + H+ =\u003e 2 glutathione (reduced) + NADP+ is a Reactome release 97 curated reaction event. The source record connects GSSG, NADPH, H+ to GSH, NADP+ in cytosol and cross-references RHEA:11742.", "equation": "GSSG + NADPH + H+ → 2 GSH + NADP+", "inputs": [ { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glutathione-disulfide reductase (NADPH) activity of GSR-2:FAD dimer [cytosol]", "activity_id": "71681", "ec_numbers": [ "1.8.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:947404", "title": "Familial deficiency of glutathione reductase in human blood cells", "journal": "Blood", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/947404/" }, { "id": "PMID:14086726", "title": "PURIFICATION AND PROPERTIES OF GLUTATHIONE REDUCTASE OF HUMAN ERYTHROCYTES", "journal": "J. Biol. Chem.", "year": 1963, "url": "https://pubmed.ncbi.nlm.nih.gov/14086726/" } ], "x": 3321.3333333333335, "y": 3123.4791666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71691", "label": "acetaldehyde + NAD+ =\u003e acetate + NADH + H+ [cytosolic]", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25295" ], "source_url": "https://reactome.org/content/detail/R-HSA-71691", "summary": "acetaldehyde + NAD+ =\u003e acetate + NADH + H+ [cytosolic] is a Reactome release 97 curated reaction event. The source record connects CH3CHO, NAD+, H2O to CH3COO-, NADH, H+ in cytosol and cross-references RHEA:25295.", "equation": "CH3CHO + NAD+ + H2O → CH3COO- + NADH + H+", "inputs": [ { "node_id": "R-ALL-29510", "reactome_id": "R-ALL-29510", "label": "CH3CHO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29416", "reactome_id": "R-ALL-29416", "label": "CH3COO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15366", "formula": "C2H4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of ALDH1A1 tetramer [cytosol]", "activity_id": "71690", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:224930", "title": "Purification and partial characterization of aldehyde dehydrogenase from human erythrocytes.", "journal": "Biochim Biophys Acta", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/224930/" }, { "id": "PMID:10631996", "title": "Human liver mitochondrial aldehyde dehydrogenase: three-dimensional structure and the restoration of solubility and activity of chimeric forms", "journal": "Protein Sci", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10631996/" } ], "x": 7461.333333333333, "y": 2852.578431372549, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71707", "label": "ethanol + NAD+ =\u003e acetaldehyde + NADH + H+", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25291" ], "source_url": "https://reactome.org/content/detail/R-HSA-71707", "summary": "ethanol + NAD+ =\u003e acetaldehyde + NADH + H+ is a Reactome release 97 curated reaction event. The source record connects EtOH, NAD+ to CH3CHO, NADH, H+ in cytosol and cross-references RHEA:25291.", "equation": "EtOH + NAD+ → CH3CHO + NADH + H+", "inputs": [ { "node_id": "R-ALL-30203", "reactome_id": "R-ALL-30203", "label": "EtOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16236", "formula": "C2H6O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29510", "reactome_id": "R-ALL-29510", "label": "CH3CHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alcohol dehydrogenase (NAD+) activity of alcohol dehydrogenase complex [cytosol]", "activity_id": "71694", "ec_numbers": [ "1.1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:427099", "title": "Human liver pi-alcohol dehydrogenase: kinetic and molecular properties.", "journal": "Biochemistry", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/427099/" }, { "id": "PMID:1755855", "title": "Enzymatic properties of the protein encoded by newly cloned human alcohol dehydrogenase ADH6 gene", "journal": "Biochem Biophys Res Commun", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1755855/" }, { "id": "PMID:10441588", "title": "Interaction between the functional polymorphisms of the alcohol-metabolism genes in protection against alcoholism.", "journal": "Am J Hum Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10441588/" }, { "id": "PMID:10697413", "title": "Regulation of the mammalian alcohol dehydrogenase genes.", "journal": "Prog Nucleic Acid Res Mol Biol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10697413/" }, { "id": "PMID:9982", "title": "Human liver alcohol dehydrogenase: purification, composition, and catalytic features.", "journal": "Biochemistry", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/9982/" }, { "id": "PMID:270680", "title": "Isolation of pi-alcohol dehydrogenase of human liver: is it a determinant of alcoholism?", "journal": "Proc Natl Acad Sci U S A", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/270680/" } ], "x": 7461.333333333333, "y": 2867.8333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71723", "label": "acetaldehyde + NAD+ =\u003e acetate + NADH + H+ [mitochondrial]", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:25295" ], "source_url": "https://reactome.org/content/detail/R-HSA-71723", "summary": "acetaldehyde + NAD+ =\u003e acetate + NADH + H+ [mitochondrial] is a Reactome release 97 curated reaction event. The source record connects CH3CHO, H2O, NAD+ to CH3COO-, H+, NADH in mitochondrial matrix and cross-references RHEA:25295.", "equation": "CH3CHO + H2O + NAD+ → CH3COO- + H+ + NADH", "inputs": [ { "node_id": "R-ALL-113532", "reactome_id": "R-ALL-113532", "label": "CH3CHO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15343", "formula": "C2H4O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113539", "reactome_id": "R-ALL-113539", "label": "CH3COO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of ALDH2 tetramer [mitochondrial matrix]", "activity_id": "71722", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:18196", "title": "Two aldehyde dehydrogenases from human liver. Isolation via affinity chromatography and characterization of the isozymes.", "journal": "Biochim Biophys Acta", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/18196/" }, { "id": "PMID:4065146", "title": "Mitochondrial aldehyde dehydrogenase from human liver. Primary structure, differences in relation to the cytosolic enzyme, and functional correlations.", "journal": "Eur J Biochem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/4065146/" }, { "id": "PMID:10631996", "title": "Human liver mitochondrial aldehyde dehydrogenase: three-dimensional structure and the restoration of solubility and activity of chimeric forms", "journal": "Protein Sci", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10631996/" } ], "x": 7740, "y": 2852.578431372549, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71735", "label": "acetate + CoA + ATP =\u003e acetyl-CoA + AMP + pyrophosphate [cytosolic]", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23177" ], "source_url": "https://reactome.org/content/detail/R-HSA-71735", "summary": "acetate + CoA + ATP =\u003e acetyl-CoA + AMP + pyrophosphate [cytosolic] is a Reactome release 97 curated reaction event. The source record connects ATP, CH3COO-, CoA-SH to AMP, PPi, Ac-CoA in cytosol and cross-references RHEA:23177.", "equation": "ATP + CH3COO- + CoA-SH → AMP + PPi + Ac-CoA", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29416", "reactome_id": "R-ALL-29416", "label": "CH3COO-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15366", "formula": "C2H4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA synthetase activity of ACSS2 [cytosol]", "activity_id": "71734", "ec_numbers": [ "6.2.1.1" ], "accessions": [ "Q9NR19" ] } ], "citations": [ { "id": "PMID:10843999", "title": "Molecular characterization of human acetyl-CoA synthetase, an enzyme regulated by sterol regulatory element-binding proteins.", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10843999/" } ], "x": 8018.666666666667, "y": 2852.578431372549, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71775", "label": "SUCLG1/G2 cleaves succinyl-CoA", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:22122" ], "source_url": "https://reactome.org/content/detail/R-HSA-71775", "summary": "SUCLG1/G2 cleaves succinyl-CoA is a Reactome release 97 curated reaction event. The source record connects GDP, Pi, SUCC-CoA to GTP, SUCCA, CoA-SH in mitochondrial matrix and cross-references RHEA:22122.", "equation": "GDP + Pi + SUCC-CoA → GTP + SUCCA + CoA-SH", "inputs": [ { "node_id": "R-ALL-113525", "reactome_id": "R-ALL-113525", "label": "GDP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58189", "formula": "C10H12N5O11P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70958", "reactome_id": "R-ALL-70958", "label": "SUCC-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57292", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113573", "reactome_id": "R-ALL-113573", "label": "GTP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:37565", "formula": "C10H12N5O14P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113536", "reactome_id": "R-ALL-113536", "label": "SUCCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30031", "formula": "C4H4O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "succinate-CoA ligase (GDP-forming) activity of SUCLG1:SUCLG2 [mitochondrial matrix]", "activity_id": "71774", "ec_numbers": [ "6.2.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:10727444", "title": "Interaction between succinyl CoA synthetase and the heme-biosynthetic enzyme ALAS-E is disrupted in sideroblastic anemia", "journal": "J Clin Invest", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10727444/" }, { "id": "PMID:9765291", "title": "Genetic evidence for the expression of ATP- and GTP-specific succinyl-CoA synthetases in multicellular eucaryotes", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9765291/" }, { "id": "PMID:9765290", "title": "Characterization of the ATP- and GTP-specific succinyl-CoA synthetases in pigeon. The enzymes incorporate the same alpha-subunit.", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9765290/" }, { "id": "PMID:15234968", "title": "Expression of two succinyl-CoA synthetases with different nucleotide specificities in mammalian tissues", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15234968/" }, { "id": "PMID:17668387", "title": "Deficiency of the alpha subunit of succinate-coenzyme A ligase causes fatal infantile lactic acidosis with mitochondrial DNA depletion", "journal": "Am J Hum Genet", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17668387/" }, { "id": "PMID:13181903", "title": "Guanosine triphosphate, the primary product of phosphorylation coupled to the breakdown of succinyl coenzyme A", "journal": "Biochim Biophys Acta", "year": 1954, "url": "https://pubmed.ncbi.nlm.nih.gov/13181903/" } ], "x": 5670, "y": 335.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71802", "label": "PFKFKB dimer phosphorylates Fru(6)P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15654" ], "source_url": "https://reactome.org/content/detail/R-HSA-71802", "summary": "PFKFKB dimer phosphorylates Fru(6)P is a Reactome release 97 curated reaction event. The source record connects Fru(6)P, ATP to D-Fructose 2,6-bisphosphate, ADP, H+ in cytosol and cross-references RHEA:15654.", "equation": "Fru(6)P + ATP → D-Fructose 2,6-bisphosphate + ADP + H+", "inputs": [ { "node_id": "R-ALL-29512", "reactome_id": "R-ALL-29512", "label": "Fru(6)P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57634", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30533", "reactome_id": "R-ALL-30533", "label": "D-Fructose 2,6-bisphosphate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58579", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "6-phosphofructo-2-kinase activity of PFKFB dimers [cytosol]", "activity_id": "163768", "ec_numbers": [ "2.7.1.105" ], "accessions": [] } ], "citations": [ { "id": "PMID:2837207", "title": "Molecular cloning, sequence analysis, and expression of a human liver cDNA coding for fructose-6-P,2-kinase:fructose-2,6-bisphosphatase", "journal": "Biochem Biophys Res Commun", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2837207/" }, { "id": "PMID:9464277", "title": "Expression of human placental-type 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various cells and cell lines", "journal": "Biochem Biophys Res Commun", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9464277/" }, { "id": "PMID:15896703", "title": "The kinase activity of human brain 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase is regulated via inhibition by phosphoenolpyruvate", "journal": "Arch Biochem Biophys", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15896703/" }, { "id": "PMID:10095107", "title": "Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene", "journal": "Gene", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10095107/" }, { "id": "PMID:3052289", "title": "Hormonal regulation of hepatic gluconeogenesis and glycolysis", "journal": "Annu Rev Biochem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3052289/" }, { "id": "PMID:7574501", "title": "6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: a metabolic signaling enzyme.", "journal": "Annu Rev Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7574501/" } ], "x": 311.33333333333337, "y": 335.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71825", "label": "G6PC hydrolyzes G6P to Glc and Pi (liver)", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "gluconeogenesis" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:44905" ], "source_url": "https://reactome.org/content/detail/R-HSA-71825", "summary": "G6PC hydrolyzes G6P to Glc and Pi (liver) is a Reactome release 97 curated reaction event. The source record connects G6P, H2O to Pi, Glc in endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:44905.", "equation": "G6P + H2O → Pi + Glc", "inputs": [ { "node_id": "R-ALL-113782", "reactome_id": "R-ALL-113782", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113551", "reactome_id": "R-ALL-113551", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113780", "reactome_id": "R-ALL-113780", "label": "Glc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucose-6-phosphatase activity of G6PC [endoplasmic reticulum membrane]", "activity_id": "71824", "ec_numbers": [ "3.1.3.9" ], "accessions": [ "P35575" ] } ], "citations": [ { "id": "PMID:7573034", "title": "Genetic basis of glycogen storage disease type 1a: Prevalent mutations at the glucose-6-phosphatase locus", "journal": "Am J Hum Genet", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7573034/" }, { "id": "PMID:12093795", "title": "The catalytic center of glucose-6-phosphatase. HIS176 is the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12093795/" }, { "id": "PMID:8211187", "title": "Mutations in the glucose-6-phosphatase gene that cause glycogen storage disease type 1a", "journal": "Science", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8211187/" }, { "id": "PMID:18449899", "title": "Mutations in the glucose-6-phosphatase-alpha (G6PC) gene that cause type Ia glycogen storage disease", "journal": "Hum. Mutat.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18449899/" } ], "x": 1251.3333333333333, "y": 619.5833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71849", "label": "LDH tetramer reduces PYR to LACT", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23446" ], "source_url": "https://reactome.org/content/detail/R-HSA-71849", "summary": "LDH tetramer reduces PYR to LACT is a Reactome release 97 curated reaction event. The source record connects PYR, NADH, H+ to LACT, NAD+ in cytosol and cross-references RHEA:23446.", "equation": "PYR + NADH + H+ → LACT + NAD+", "inputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29708", "reactome_id": "R-ALL-29708", "label": "LACT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16651", "formula": "C3H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-lactate dehydrogenase (NAD+) activity of LDH tetramer [cytosol]", "activity_id": "70509", "ec_numbers": [ "1.1.1.27" ], "accessions": [] } ], "citations": [ { "id": "PMID:18351441", "title": "Identification of a novel human lactate dehydrogenase gene LDHAL6A, which activates transcriptional activities of AP1(PMA)", "journal": "Mol. Biol. Rep.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18351441/" }, { "id": "PMID:1996957", "title": "Properties of human testis-specific lactate dehydrogenase expressed from Escherichia coli", "journal": "Biochem. J.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1996957/" }, { "id": "PMID:2440048", "title": "Epitopes of human testis-specific lactate dehydrogenase deduced from a cDNA sequence", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2440048/" }, { "id": "PMID:11276087", "title": "Structural basis for altered activity of M- and H-isozyme forms of human lactate dehydrogenase", "journal": "Proteins", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11276087/" }, { "id": "PMID:3435492", "title": "The cDNA and protein sequences of human lactate dehydrogenase B", "journal": "Biochem J", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3435492/" }, { "id": "PMID:11377399", "title": "Selective active site inhibitors of human lactate dehydrogenases A4, B4, and C4", "journal": "Biochem Pharmacol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11377399/" } ], "x": 4451.333333333333, "y": 321.35, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-71850", "label": "PGK complexes dephosphorylate 1,3BPG", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14803" ], "source_url": "https://reactome.org/content/detail/R-HSA-71850", "summary": "PGK complexes dephosphorylate 1,3BPG is a Reactome release 97 curated reaction event. The source record connects 1,3BPG, ADP to 3PG, ATP in cytosol and cross-references RHEA:14803.", "equation": "1,3BPG + ADP → 3PG + ATP", "inputs": [ { "node_id": "R-ALL-29800", "reactome_id": "R-ALL-29800", "label": "1,3BPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57604", "formula": "C3H4O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29728", "reactome_id": "R-ALL-29728", "label": "3PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58272", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoglycerate kinase activity of PGK complexes [cytosol]", "activity_id": "70485", "ec_numbers": [ "2.7.2.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:1278465", "title": "Characterization of phosphoglycerate kinase from human spermatozoa", "journal": "Fertil. Steril.", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/1278465/" }, { "id": "PMID:6771269", "title": "Complete amino acid sequence of human phosphoglycerate kinase. Isolation and amino acid sequence of tryptic peptides.", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6771269/" }, { "id": "PMID:7391027", "title": "Complete amino acid sequence of human phosphoglycerate kinase. Cyanogen bromide peptides and complete amino acid sequence.", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/7391027/" }, { "id": "PMID:3453121", "title": "Human testis-specific PGK gene lacks introns and possesses characteristics of a processed gene", "journal": "Nature", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3453121/" }, { "id": "PMID:5009693", "title": "Human phosphoglycerate kinase. I. Crystallization and characterization of normal enzyme", "journal": "J Biol Chem", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/5009693/" } ], "x": 590, "y": 384.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73548", "label": "(d)CMP or UMP + ATP (d)CDP or UDP + ADP (CMPK1)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24401", "RHEA:25095", "RHEA:11601" ], "source_url": "https://reactome.org/content/detail/R-HSA-73548", "summary": "(d)CMP or UMP + ATP (d)CDP or UDP + ADP (CMPK1) is a Reactome release 97 curated reaction event. The source record connects (d)CMP, UMP, ATP to (d)CDP, UDP, ADP in cytosol and cross-references RHEA:24401, RHEA:25095, RHEA:11601.", "equation": "(d)CMP, UMP + ATP → (d)CDP, UDP + ADP", "inputs": [ { "node_id": "R-ALL-500009", "reactome_id": "R-ALL-500009", "label": "(d)CMP, UMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500006", "reactome_id": "R-ALL-500006", "label": "(d)CDP, UDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside monophosphate kinase activity of CMPK1, AK1 [cytosol]", "activity_id": "73495", "ec_numbers": [ "2.7.4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:11912132", "title": "Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates.", "journal": "Cancer Res", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11912132/" }, { "id": "PMID:40615", "title": "Kinetics and equilibria of pyrimidine nucleoside monophosphate kinase from human erythrocytes", "journal": "Biochim Biophys Acta", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/40615/" } ], "x": 3878.666666666667, "y": 3086.1041666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73564", "label": "UMPS dimer decarboxylates OMP to UMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11597" ], "source_url": "https://reactome.org/content/detail/R-HSA-73564", "summary": "UMPS dimer decarboxylates OMP to UMP is a Reactome release 97 curated reaction event. The source record connects OMP, H+ to UMP, CO2 in cytosol and cross-references RHEA:11597.", "equation": "OMP + H+ → UMP + CO2", "inputs": [ { "node_id": "R-ALL-111629", "reactome_id": "R-ALL-111629", "label": "OMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57538", "formula": "C10H10N2O11P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-109402", "reactome_id": "R-ALL-109402", "label": "UMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57865", "formula": "C9H11N2O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "orotidine-5'-phosphate decarboxylase activity of UMPS dimer [cytosol]", "activity_id": "73492", "ec_numbers": [ "4.1.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:9042911", "title": "Molecular cloning of the human UMP synthase gene and characterization of point mutations in two hereditary orotic aciduria families", "journal": "Am J Hum Genet", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9042911/" }, { "id": "PMID:8631878", "title": "Intrinsic activity and stability of bifunctional human UMP synthase and its two separate catalytic domains, orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631878/" }, { "id": "PMID:18184586", "title": "Structures of the human orotidine-5'-monophosphate decarboxylase support a covalent mechanism and provide a framework for drug design", "journal": "Structure", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18184586/" }, { "id": "PMID:6893554", "title": "Isolation and initial characterization of the single polypeptide that synthesizes uridine 5'-monophosphate from orotate in Ehrlich ascites carcinoma. Purification by tandem affinity chromatography of uridine-5'-monophosphate synthase", "journal": "Biochemistry", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6893554/" } ], "x": 2660, "y": 2868.40625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73567", "label": "UMPS dimer transfers phosphoribosyl group to ORO to form OMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10382" ], "source_url": "https://reactome.org/content/detail/R-HSA-73567", "summary": "UMPS dimer transfers phosphoribosyl group to ORO to form OMP is a Reactome release 97 curated reaction event. The source record connects ORO, PRPP to OMP, PPi in cytosol and cross-references RHEA:10382.", "equation": "ORO + PRPP → OMP + PPi", "inputs": [ { "node_id": "R-ALL-76632", "reactome_id": "R-ALL-76632", "label": "ORO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30839", "formula": "C5H3N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111629", "reactome_id": "R-ALL-111629", "label": "OMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57538", "formula": "C10H10N2O11P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "orotate phosphoribosyltransferase activity of UMPS dimer [cytosol]", "activity_id": "73491", "ec_numbers": [ "2.4.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:9042911", "title": "Molecular cloning of the human UMP synthase gene and characterization of point mutations in two hereditary orotic aciduria families", "journal": "Am J Hum Genet", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9042911/" }, { "id": "PMID:8631878", "title": "Intrinsic activity and stability of bifunctional human UMP synthase and its two separate catalytic domains, orotate phosphoribosyltransferase and orotidine-5'-phosphate decarboxylase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631878/" }, { "id": "PMID:6893554", "title": "Isolation and initial characterization of the single polypeptide that synthesizes uridine 5'-monophosphate from orotate in Ehrlich ascites carcinoma. Purification by tandem affinity chromatography of uridine-5'-monophosphate synthase", "journal": "Biochemistry", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6893554/" }, { "id": "PMID:18184586", "title": "Structures of the human orotidine-5'-monophosphate decarboxylase support a covalent mechanism and provide a framework for drug design", "journal": "Structure", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18184586/" } ], "x": 2938.666666666667, "y": 2868.40625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73569", "label": "DHODH:FMN oxidises (S)-DHO to orotate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol · mitochondrial inner membrane", "compartment_ids": [ "GO:0005829", "GO:0005743" ], "rhea_ids": [ "RHEA:28692" ], "source_url": "https://reactome.org/content/detail/R-HSA-73569", "summary": "DHODH:FMN oxidises (S)-DHO to orotate is a Reactome release 97 curated reaction event. The source record connects (S)-DHO, CoQ to ORO, QH2 in cytosol · mitochondrial inner membrane and cross-references RHEA:28692.", "equation": "(S)-DHO + CoQ → ORO + QH2", "inputs": [ { "node_id": "R-ALL-76630", "reactome_id": "R-ALL-76630", "label": "(S)-DHO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30864", "formula": "C5H5N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73663", "reactome_id": "R-ALL-73663", "label": "CoQ", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46245", "formula": "C59H90O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76632", "reactome_id": "R-ALL-76632", "label": "ORO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30839", "formula": "C5H3N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76633", "reactome_id": "R-ALL-76633", "label": "QH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17976", "formula": "(C5H8)n.C9H12O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dihydroorotate dehydrogenase activity of DHODH:FMN [mitochondrial inner membrane]", "activity_id": "73535", "ec_numbers": [ "1.3.5.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:6105839", "title": "Pyrimidine nucleotide biosynthesis in animals: genes, enzymes, and regulation of UMP biosynthesis.", "journal": "Annu Rev Biochem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6105839/" }, { "id": "PMID:165196", "title": "Superoxide production and electron transport in mitochondrial oxidation of dihydroorotic acid.", "journal": "J Biol Chem", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/165196/" }, { "id": "PMID:9693067", "title": "Expression, purification, and characterization of histidine-tagged rat and human flavoenzyme dihydroorotate dehydrogenase.", "journal": "Protein Expr Purif", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9693067/" }, { "id": "PMID:10889450", "title": "Indirect inhibition of mitochondrial dihydroorotate dehydrogenase activity by nitric oxide.", "journal": "Free Radic Biol Med", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10889450/" }, { "id": "PMID:13115431", "title": "Enzymic synthesis and breakdown of a pyrimidine, orotic acid. I. Dihydro-orotic dehydrogenase", "journal": "Biochim Biophys Acta", "year": 1953, "url": "https://pubmed.ncbi.nlm.nih.gov/13115431/" } ], "x": 2938.666666666667, "y": 2905.78125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73571", "label": "CAD hexamer dehydrates N-carb-L-Asp to (S)-DHO", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24298" ], "source_url": "https://reactome.org/content/detail/R-HSA-73571", "summary": "CAD hexamer dehydrates N-carb-L-Asp to (S)-DHO is a Reactome release 97 curated reaction event. The source record connects N-carb-L-Asp, H+ to (S)-DHO, H2O in cytosol and cross-references RHEA:24298.", "equation": "N-carb-L-Asp + H+ → (S)-DHO + H2O", "inputs": [ { "node_id": "R-ALL-111628", "reactome_id": "R-ALL-111628", "label": "N-carb-L-Asp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32814", "formula": "C5H6N2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76630", "reactome_id": "R-ALL-76630", "label": "(S)-DHO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30864", "formula": "C5H5N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dihydroorotase activity of CAD hexamer [cytosol]", "activity_id": "73460", "ec_numbers": [ "3.5.2.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:8619816", "title": "Molecular cloning of a human cDNA encoding a trifunctional enzyme of carbamoyl-phosphate synthetase-aspartate transcarbamoylase-dihydroorotase in de Novo pyrimidine synthesis", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8619816/" } ], "x": 2381.3333333333335, "y": 2887.09375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73573", "label": "CAD hexamer transforms CAP to N-carb-L-Asp", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20014" ], "source_url": "https://reactome.org/content/detail/R-HSA-73573", "summary": "CAD hexamer transforms CAP to N-carb-L-Asp is a Reactome release 97 curated reaction event. The source record connects CAP, L-Asp to N-carb-L-Asp, Pi, H+ in cytosol and cross-references RHEA:20014.", "equation": "CAP + L-Asp → N-carb-L-Asp + Pi + H+", "inputs": [ { "node_id": "R-ALL-111647", "reactome_id": "R-ALL-111647", "label": "CAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58228", "formula": "CH2NO5P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29448", "reactome_id": "R-ALL-29448", "label": "L-Asp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111628", "reactome_id": "R-ALL-111628", "label": "N-carb-L-Asp", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32814", "formula": "C5H6N2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aspartate carbamoyltransferase activity of CAD hexamer [cytosol]", "activity_id": "73459", "ec_numbers": [ "2.1.3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:4684686", "title": "Control of pyrimidine biosynthesis in human lymphocytes. Simultaneous increase in activities of glutamine-utilizing carbamyl phosphate synthetase and aspartate transcarbamylase in phytohemagglutinin-stimulated human peripheral lymphocytes and their enzyme co-purification", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4684686/" }, { "id": "PMID:8619816", "title": "Molecular cloning of a human cDNA encoding a trifunctional enzyme of carbamoyl-phosphate synthetase-aspartate transcarbamoylase-dihydroorotase in de Novo pyrimidine synthesis", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8619816/" }, { "id": "PMID:2995985", "title": "Oligomeric structure of the multifunctional protein CAD that initiates pyrimidine biosynthesis in mammalian cells", "journal": "Proc Natl Acad Sci USA", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2995985/" } ], "x": 2381.3333333333335, "y": 2868.40625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73577", "label": "CAD hexamer transforms L-Gln to CAP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18634" ], "source_url": "https://reactome.org/content/detail/R-HSA-73577", "summary": "CAD hexamer transforms L-Gln to CAP is a Reactome release 97 curated reaction event. The source record connects HCO3-, L-Gln, ATP to CAP, L-Glu, ADP in cytosol and cross-references RHEA:18634.", "equation": "HCO3- + L-Gln + 2 ATP + H2O → CAP + L-Glu + 2 ADP + Pi + 2 H+", "inputs": [ { "node_id": "R-ALL-111627", "reactome_id": "R-ALL-111627", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111647", "reactome_id": "R-ALL-111647", "label": "CAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58228", "formula": "CH2NO5P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity of CAD hexamer [cytosol]", "activity_id": "73458", "ec_numbers": [ "6.3.5.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:4684686", "title": "Control of pyrimidine biosynthesis in human lymphocytes. Simultaneous increase in activities of glutamine-utilizing carbamyl phosphate synthetase and aspartate transcarbamylase in phytohemagglutinin-stimulated human peripheral lymphocytes and their enzyme co-purification", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4684686/" }, { "id": "PMID:8619816", "title": "Molecular cloning of a human cDNA encoding a trifunctional enzyme of carbamoyl-phosphate synthetase-aspartate transcarbamoylase-dihydroorotase in de Novo pyrimidine synthesis", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8619816/" } ], "x": 2381.3333333333335, "y": 2812.34375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73580", "label": "PRPS dimers pyrophosphorylate R5P using ATP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15610" ], "source_url": "https://reactome.org/content/detail/R-HSA-73580", "summary": "PRPS dimers pyrophosphorylate R5P using ATP is a Reactome release 97 curated reaction event. The source record connects R5P, ATP to PRPP, AMP, H+ in cytosol and cross-references RHEA:15610.", "equation": "R5P + ATP → PRPP + AMP + H+", "inputs": [ { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribose phosphate diphosphokinase activity of PRPS dimers [cytosol]", "activity_id": "111174", "ec_numbers": [ "2.7.6.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:4328836", "title": "Human phosphoribosylpyrophosphate synthetase", "journal": "J Biol Chem", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/4328836/" }, { "id": "PMID:28031352", "title": "Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance", "journal": "Microbiol Mol Biol Rev", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28031352/" } ], "x": 590, "y": 769.7857142857143, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73585", "label": "reduction of uracil to form dihydrouracil", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18095" ], "source_url": "https://reactome.org/content/detail/R-HSA-73585", "summary": "reduction of uracil to form dihydrouracil is a Reactome release 97 curated reaction event. The source record connects Ura, NADPH, H+ to 5,6-Dihydrouracil, NADP+ in cytosol and cross-references RHEA:18095.", "equation": "Ura + NADPH + H+ → 5,6-Dihydrouracil + NADP+", "inputs": [ { "node_id": "R-ALL-83962", "reactome_id": "R-ALL-83962", "label": "Ura", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17568", "formula": "C4H4N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73584", "reactome_id": "R-ALL-73584", "label": "5,6-Dihydrouracil", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15901", "formula": "C4H6N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dihydropyrimidine dehydrogenase (NADP+) activity of DPYD dimer [cytosol]", "activity_id": "73530", "ec_numbers": [ "1.3.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8083224", "title": "cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8083224/" } ], "x": 2938.666666666667, "y": 3124.3690476190477, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73589", "label": "conversion of 5,6-dihydrouracil to 3-ureidopropionate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16122" ], "source_url": "https://reactome.org/content/detail/R-HSA-73589", "summary": "conversion of 5,6-dihydrouracil to 3-ureidopropionate is a Reactome release 97 curated reaction event. The source record connects 5,6-Dihydrouracil, H2O to UPROP, H+ in cytosol and cross-references RHEA:16122.", "equation": "5,6-Dihydrouracil + H2O → UPROP + H+", "inputs": [ { "node_id": "R-ALL-73584", "reactome_id": "R-ALL-73584", "label": "5,6-Dihydrouracil", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15901", "formula": "C4H6N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73588", "reactome_id": "R-ALL-73588", "label": "UPROP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11892", "formula": "C4H7N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dihydropyrimidinase activity of DPYS tetramer [cytosol]", "activity_id": "73469", "ec_numbers": [ "3.5.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9718352", "title": "Dihydropyrimidinase deficiency: structural organization, chromosomal localization, and mutation analysis of the human dihydropyrimidinase gene", "journal": "Am J Hum Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9718352/" } ], "x": 2938.666666666667, "y": 3159.964285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73591", "label": "conversion of 3-ureidopropionate to beta-alanine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11185" ], "source_url": "https://reactome.org/content/detail/R-HSA-73591", "summary": "conversion of 3-ureidopropionate to beta-alanine is a Reactome release 97 curated reaction event. The source record connects UPROP, H2O, H+ to b-Ala, CO2, NH4+ in cytosol and cross-references RHEA:11185.", "equation": "UPROP + H2O + 2 H+ → b-Ala + CO2 + NH4+", "inputs": [ { "node_id": "R-ALL-73588", "reactome_id": "R-ALL-73588", "label": "UPROP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11892", "formula": "C4H7N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73590", "reactome_id": "R-ALL-73590", "label": "b-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57966", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-ureidopropionase activity of UPB1:Zn2+ [cytosol]", "activity_id": "73466", "ec_numbers": [ "3.5.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:15385443", "title": "beta-Ureidopropionase deficiency: an inborn error of pyrimidine degradation associated with neurological abnormalities", "journal": "Hum Mol Genet", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15385443/" } ], "x": 2660, "y": 3081.654761904762, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73596", "label": "dCMP + H2O =\u003e dUMP + NH4+", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22925" ], "source_url": "https://reactome.org/content/detail/R-HSA-73596", "summary": "dCMP + H2O =\u003e dUMP + NH4+ is a Reactome release 97 curated reaction event. The source record connects dCMP, H2O, H+ to dUMP, NH4+ in cytosol and cross-references RHEA:22925.", "equation": "dCMP + H2O + H+ → dUMP + NH4+", "inputs": [ { "node_id": "R-ALL-109378", "reactome_id": "R-ALL-109378", "label": "dCMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57566", "formula": "C9H12N3O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-109382", "reactome_id": "R-ALL-109382", "label": "dUMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:246422", "formula": "C9H11N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dCMP deaminase activity of DCTD hexamer [cytosol]", "activity_id": "73593", "ec_numbers": [ "3.5.4.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:7685356", "title": "Primary structure of human deoxycytidylate deaminase and overexpression of its functional protein in Escherichia coli", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7685356/" }, { "id": "PMID:8448179", "title": "Properties of an affinity-column-purified human deoxycytidylate deaminase", "journal": "Biochim Biophys Acta", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8448179/" } ], "x": 3600, "y": 3123.4791666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73598", "label": "(2'-deoxy)cytidine + ATP =\u003e (d)CMP + ADP (DCK)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:46041", "RHEA:24675" ], "source_url": "https://reactome.org/content/detail/R-HSA-73598", "summary": "(2'-deoxy)cytidine + ATP =\u003e (d)CMP + ADP (DCK) is a Reactome release 97 curated reaction event. The source record connects Cyt-Rib, dC, ATP to (d)CMP, ADP, H+ in cytosol and cross-references RHEA:46041, RHEA:24675.", "equation": "Cyt-Rib, dC + ATP → (d)CMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-500829", "reactome_id": "R-ALL-500829", "label": "Cyt-Rib, dC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500828", "reactome_id": "R-ALL-500828", "label": "(d)CMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside kinase activity of DCK dimer [cytosol]", "activity_id": "73517", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:2844225", "title": "Deoxycytidine kinase from human leukemic spleen: preparation and characterization of the homogeneous enzyme", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2844225/" }, { "id": "PMID:1996353", "title": "Cloning and expression of human deoxycytidine kinase cDNA", "journal": "Proc Natl Acad Sci USA", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1996353/" }, { "id": "PMID:2539852", "title": "Human T-lymphoblast deoxycytidine kinase: purification and properties", "journal": "Biochemistry", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2539852/" }, { "id": "PMID:2542307", "title": "Kinetic properties and inhibition of human T lymphoblast deoxycytidine kinase", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2542307/" }, { "id": "PMID:9804782", "title": "The intracellular localization of deoxycytidine kinase", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9804782/" }, { "id": "PMID:12808445", "title": "Structure of human dCK suggests strategies to improve anticancer and antiviral therapy", "journal": "Nat Struct Biol", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12808445/" } ], "x": 2381.3333333333335, "y": 3596.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73599", "label": "cytidine or uridine + ATP =\u003e CMP or UMP + ADP [UCK1]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24675", "RHEA:16826" ], "source_url": "https://reactome.org/content/detail/R-HSA-73599", "summary": "cytidine or uridine + ATP =\u003e CMP or UMP + ADP [UCK1] is a Reactome release 97 curated reaction event. The source record connects Cyt-Rib, Ura-Rib, ATP to CMP, UMP, ADP, H+ in cytosol and cross-references RHEA:24675, RHEA:16826.", "equation": "Cyt-Rib, Ura-Rib + ATP → CMP, UMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-500759", "reactome_id": "R-ALL-500759", "label": "Cyt-Rib, Ura-Rib", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500761", "reactome_id": "R-ALL-500761", "label": "CMP, UMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside kinase activity of UCK1 tetramer [cytosol]", "activity_id": "73505", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:195585", "title": "Uridine kinase activities and pyrimidine nucleoside phosphorylation in fluoropyrimidine-sensitive and -resistant cell lines of the Novikoff hepatoma", "journal": "Biochem J", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/195585/" }, { "id": "PMID:11306702", "title": "Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases", "journal": "Mol Pharmacol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11306702/" } ], "x": 3321.3333333333335, "y": 2899.1071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73605", "label": "conversion of dUMP to dTMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12105" ], "source_url": "https://reactome.org/content/detail/R-HSA-73605", "summary": "conversion of dUMP to dTMP is a Reactome release 97 curated reaction event. The source record connects dUMP, 5,10-methylenetetrahydrofolate(2-) to DHF, TMP in cytosol and cross-references RHEA:12105.", "equation": "dUMP + 5,10-methylenetetrahydrofolate(2-) → DHF + TMP", "inputs": [ { "node_id": "R-ALL-109382", "reactome_id": "R-ALL-109382", "label": "dUMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:246422", "formula": "C9H11N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1132205", "reactome_id": "R-ALL-1132205", "label": "5,10-methylenetetrahydrofolate(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15636", "formula": "C20H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73604", "reactome_id": "R-ALL-73604", "label": "DHF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57451", "formula": "C19H19N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109366", "reactome_id": "R-ALL-109366", "label": "TMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63528", "formula": "C10H13N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "thymidylate synthase activity of TS dimer [cytosol]", "activity_id": "73520", "ec_numbers": [ "2.1.1.45" ], "accessions": [] } ], "citations": [ { "id": "PMID:2656695", "title": "Expression of human thymidylate synthase in Escherichia coli", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2656695/" }, { "id": "PMID:11278511", "title": "Structure of human thymidylate synthase suggests advantages of chemotherapy with noncompetitive inhibitors", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11278511/" } ], "x": 3878.666666666667, "y": 3148.3958333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73608", "label": "deamination of 2'-Deoxycytidine to 2'-Deoxyuridine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13434", "RHEA:16070" ], "source_url": "https://reactome.org/content/detail/R-HSA-73608", "summary": "deamination of 2'-Deoxycytidine to 2'-Deoxyuridine is a Reactome release 97 curated reaction event. The source record connects Cyt-Rib, dC, H2O, H+ to (d)Ura, NH4+ in cytosol and cross-references RHEA:13434, RHEA:16070.", "equation": "Cyt-Rib, dC + H2O + H+ → (d)Ura + NH4+", "inputs": [ { "node_id": "R-ALL-500829", "reactome_id": "R-ALL-500829", "label": "Cyt-Rib, dC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500430", "reactome_id": "R-ALL-500430", "label": "(d)Ura", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cytidine deaminase activity of CDA tetramer [cytosol]", "activity_id": "73463", "ec_numbers": [ "3.5.4.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:15689149", "title": "Structure of human cytidine deaminase bound to a potent inhibitor", "journal": "J Med Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15689149/" }, { "id": "PMID:7923172", "title": "Human cytidine deaminase: purification of enzyme, cloning, and expression of its complementary DNA", "journal": "Cancer Res", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7923172/" } ], "x": 3321.3333333333335, "y": 2813.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73616", "label": "reduction of thymine to form 5,6-Dihydrothymine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:58286" ], "source_url": "https://reactome.org/content/detail/R-HSA-73616", "summary": "reduction of thymine to form 5,6-Dihydrothymine is a Reactome release 97 curated reaction event. The source record connects Thy, NADPH, H+ to 5,6-Dihydrothymine, NADP+ in cytosol and cross-references RHEA:58286.", "equation": "Thy + NADPH + H+ → 5,6-Dihydrothymine + NADP+", "inputs": [ { "node_id": "R-ALL-83960", "reactome_id": "R-ALL-83960", "label": "Thy", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17821", "formula": "C5H6N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73541", "reactome_id": "R-ALL-73541", "label": "5,6-Dihydrothymine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27468", "formula": "C5H8N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dihydropyrimidine dehydrogenase (NADP+) activity of DPYD dimer [cytosol]", "activity_id": "73530", "ec_numbers": [ "1.3.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8083224", "title": "cDNA cloning and chromosome mapping of human dihydropyrimidine dehydrogenase, an enzyme associated with 5-fluorouracil toxicity and congenital thymine uraciluria", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8083224/" } ], "x": 2381.3333333333335, "y": 3131.4880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73618", "label": "conversion of 5,6-Dihydrothymine to 3-Ureidoisobutyrate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:37336" ], "source_url": "https://reactome.org/content/detail/R-HSA-73618", "summary": "conversion of 5,6-Dihydrothymine to 3-Ureidoisobutyrate is a Reactome release 97 curated reaction event. The source record connects 5,6-Dihydrothymine, H2O to UIBA, H+ in cytosol and cross-references RHEA:37336.", "equation": "5,6-Dihydrothymine + H2O → UIBA + H+", "inputs": [ { "node_id": "R-ALL-73541", "reactome_id": "R-ALL-73541", "label": "5,6-Dihydrothymine", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27468", "formula": "C5H8N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73617", "reactome_id": "R-ALL-73617", "label": "UIBA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:74414", "formula": "C5H9N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dihydropyrimidinase activity of DPYS tetramer [cytosol]", "activity_id": "73469", "ec_numbers": [ "3.5.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:9718352", "title": "Dihydropyrimidinase deficiency: structural organization, chromosomal localization, and mutation analysis of the human dihydropyrimidinase gene", "journal": "Am J Hum Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9718352/" } ], "x": 2381.3333333333335, "y": 3167.0833333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73620", "label": "conversion of 3-Ureidoiosbutyrate to 3-Aminoisobutyrate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:37340" ], "source_url": "https://reactome.org/content/detail/R-HSA-73620", "summary": "conversion of 3-Ureidoiosbutyrate to 3-Aminoisobutyrate is a Reactome release 97 curated reaction event. The source record connects UIBA, H2O, H+ to CO2, NH4+, 3AIB in cytosol and cross-references RHEA:37340.", "equation": "UIBA + H2O + 2 H+ → CO2 + NH4+ + 3AIB", "inputs": [ { "node_id": "R-ALL-73617", "reactome_id": "R-ALL-73617", "label": "UIBA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:74414", "formula": "C5H9N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73619", "reactome_id": "R-ALL-73619", "label": "3AIB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57731", "formula": "C4H9NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-ureidopropionase activity of UPB1:Zn2+ [cytosol]", "activity_id": "73466", "ec_numbers": [ "3.5.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:10989446", "title": "Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver", "journal": "Methods Enzymol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10989446/" }, { "id": "PMID:15385443", "title": "beta-Ureidopropionase deficiency: an inborn error of pyrimidine degradation associated with neurological abnormalities", "journal": "Hum Mol Genet", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15385443/" } ], "x": 2938.666666666667, "y": 3081.654761904762, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73632", "label": "phosphorylation of 2'-Deoxythymidine to dTMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19130" ], "source_url": "https://reactome.org/content/detail/R-HSA-73632", "summary": "phosphorylation of 2'-Deoxythymidine to dTMP is a Reactome release 97 curated reaction event. The source record connects Thy-dRib, ATP to TMP, ADP, H+ in cytosol and cross-references RHEA:19130.", "equation": "Thy-dRib + ATP → TMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-83928", "reactome_id": "R-ALL-83928", "label": "Thy-dRib", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17748", "formula": "C10H14N2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-109366", "reactome_id": "R-ALL-109366", "label": "TMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63528", "formula": "C10H13N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside kinase activity of TK1 tetramer [cytosol]", "activity_id": "73502", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15733844", "title": "Structure of a type II thymidine kinase with bound dTTP", "journal": "FEBS Lett", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15733844/" }, { "id": "PMID:1063125", "title": "Human deoxythymidine kinase. I. Purification and general properties of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia", "journal": "J Biol Chem", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/1063125/" }, { "id": "PMID:2026611", "title": "Diverging substrate specificity of pure human thymidine kinases 1 and 2 against antiviral dideoxynucleosides", "journal": "J Biol Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2026611/" }, { "id": "PMID:8340387", "title": "Reversible ATP-dependent transition between two forms of human cytosolic thymidine kinase with different enzymatic properties", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8340387/" }, { "id": "PMID:3372530", "title": "Regulation of human thymidine kinase during the cell cycle", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3372530/" }, { "id": "PMID:3335503", "title": "Human cytosolic thymidine kinase. Purification and physical characterization of the enzyme from HeLa cells", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3335503/" } ], "x": 3321.3333333333335, "y": 2856.3928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73635", "label": "dUMP or TMP + ATP dUDP or TDP + ADP [DTYMK]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:30656", "RHEA:13518" ], "source_url": "https://reactome.org/content/detail/R-HSA-73635", "summary": "dUMP or TMP + ATP dUDP or TDP + ADP [DTYMK] is a Reactome release 97 curated reaction event. The source record connects dUMP, TMP, ATP to dUDP, TDP, ADP in cytosol and cross-references RHEA:30656, RHEA:13518.", "equation": "dUMP, TMP + ATP → dUDP, TDP + ADP", "inputs": [ { "node_id": "R-ALL-499997", "reactome_id": "R-ALL-499997", "label": "dUMP, TMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-499999", "reactome_id": "R-ALL-499999", "label": "dUDP, TDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside monophosphate kinase activity of DTYMK dimer [cytosol]", "activity_id": "73499", "ec_numbers": [ "2.7.4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:18469", "title": "Human thymidylate kinase. Purification, characterization, and kinetic behavior of the thymidylate kinase derived from chronic myelocytic leukemia", "journal": "J Biol Chem", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/18469/" } ], "x": 3321.3333333333335, "y": 3148.3958333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73646", "label": "Regeneration of active (reduced) Thioredoxin", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20347" ], "source_url": "https://reactome.org/content/detail/R-HSA-73646", "summary": "Regeneration of active (reduced) Thioredoxin is a Reactome release 97 curated reaction event. The source record connects 2xHC-TXN, NADPH, H+ to TXN, NADP+ in cytosol and cross-references RHEA:20347.", "equation": "2xHC-TXN + NADPH + H+ → TXN + NADP+", "inputs": [ { "node_id": "R-HSA-73668", "reactome_id": "R-HSA-73668", "label": "2xHC-TXN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-66000", "reactome_id": "R-HSA-66000", "label": "TXN", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "thioredoxin-disulfide reductase (NADPH) activity of TNXRD1:FAD dimer [cytosol]", "activity_id": "73533", "ec_numbers": [ "1.8.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:16750198", "title": "Truncated mutants of human thioredoxin reductase 1 do not exhibit glutathione reductase activity", "journal": "FEBS Lett", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16750198/" } ], "x": 3878.666666666667, "y": 3160.8541666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73647", "label": "Amination of uridine 5'-triphosphate (UTP) to form cytidine 5'-triphosphate (CTPS)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:26427" ], "source_url": "https://reactome.org/content/detail/R-HSA-73647", "summary": "Amination of uridine 5'-triphosphate (UTP) to form cytidine 5'-triphosphate (CTPS) is a Reactome release 97 curated reaction event. The source record connects ATP, H2O, L-Gln to ADP, CTP, L-Glu in cytosol and cross-references RHEA:26427.", "equation": "ATP + H2O + L-Gln + UTP → ADP + CTP + L-Glu + Pi + 2 H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29494", "reactome_id": "R-ALL-29494", "label": "UTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46398", "formula": "C9H11N2O15P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-110614", "reactome_id": "R-ALL-110614", "label": "CTP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:37563", "formula": "C9H12N3O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "CTP synthase activity of CTPS tetramer [cytosol]", "activity_id": "73537", "ec_numbers": [ "6.3.4.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:16179339", "title": "Expression of Human CTP synthetase in Saccharomyces cerevisiae reveals phosphorylation by protein kinase A", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16179339/" }, { "id": "PMID:16820675", "title": "Structure of the synthetase domain of human CTP synthetase, a target for anticancer therapy", "journal": "Acta Crystallogr Sect F Struct Biol Cryst Commun", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16820675/" } ], "x": 3600, "y": 3111.0208333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73666", "label": "hydrolysis of 2'-deoxyuridine 5'-triphosphate to form 2'-deoxyuridine 5'-phosphate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:10249" ], "source_url": "https://reactome.org/content/detail/R-HSA-73666", "summary": "hydrolysis of 2'-deoxyuridine 5'-triphosphate to form 2'-deoxyuridine 5'-phosphate is a Reactome release 97 curated reaction event. The source record connects dUTP, H2O to dUMP, PPi, H+ in nucleoplasm and cross-references RHEA:10249.", "equation": "dUTP + H2O → dUMP + PPi + H+", "inputs": [ { "node_id": "R-ALL-500739", "reactome_id": "R-ALL-500739", "label": "dUTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61555", "formula": "C9H11N2O14P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113518", "reactome_id": "R-ALL-113518", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500742", "reactome_id": "R-ALL-500742", "label": "dUMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:246422", "formula": "C9H11N2O8P", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113541", "reactome_id": "R-ALL-113541", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dUTP diphosphatase activity of DUT trimer [nucleoplasm]", "activity_id": "142075", "ec_numbers": [ "3.6.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:8805593", "title": "Human dUTP pyrophosphatase: uracil recognition by a beta hairpin and active sites formed by three separate subunits", "journal": "Structure", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8805593/" }, { "id": "PMID:8631816", "title": "Characterization of distinct nuclear and mitochondrial forms of human deoxyuridine triphosphate nucleotidohydrolase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631816/" } ], "x": 3878.666666666667, "y": 3123.4791666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73788", "label": "(d)GMP + ATP (d)GDP + ADP (GUK1)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12698", "RHEA:20781" ], "source_url": "https://reactome.org/content/detail/R-HSA-73788", "summary": "(d)GMP + ATP (d)GDP + ADP (GUK1) is a Reactome release 97 curated reaction event. The source record connects (d)GMP, ATP to (d)GDP, ADP in cytosol and cross-references RHEA:12698, RHEA:20781.", "equation": "(d)GMP + ATP → (d)GDP + ADP", "inputs": [ { "node_id": "R-ALL-500071", "reactome_id": "R-ALL-500071", "label": "(d)GMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500077", "reactome_id": "R-ALL-500077", "label": "(d)GDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "GMP kinase activity of GUK1 [cytosol]", "activity_id": "74189", "ec_numbers": [ "2.7.4.8" ], "accessions": [ "Q16774" ] } ], "citations": [ { "id": "PMID:211390", "title": "Guanylate kinases from human erythrocytes, hog brain, and rat liver", "journal": "Methods Enzymol", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/211390/" }, { "id": "PMID:8663313", "title": "Cloning, characterization, and modeling of mouse and human guanylate kinases", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8663313/" }, { "id": "PMID:177353", "title": "Studies on the properties and tissue distribution of the isozymes of guanylate kinase in man", "journal": "Human Hered", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/177353/" }, { "id": "PMID:6248551", "title": "Phosphorylation of acyclovir (acycloguanosine) monophosphate by GMP kinase", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6248551/" } ], "x": 3321.3333333333335, "y": 3098.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73792", "label": "XMP + L-Glutamine + ATP + H2O =\u003e GMP + L-Glutamate + AMP + pyrophosphate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11681" ], "source_url": "https://reactome.org/content/detail/R-HSA-73792", "summary": "XMP + L-Glutamine + ATP + H2O =\u003e GMP + L-Glutamate + AMP + pyrophosphate is a Reactome release 97 curated reaction event. The source record connects XMP, L-Gln, ATP to GMP, L-Glu, H+ in cytosol and cross-references RHEA:11681.", "equation": "XMP + L-Gln + ATP + H2O → GMP + L-Glu + 2 H+ + AMP + PPi", "inputs": [ { "node_id": "R-ALL-111584", "reactome_id": "R-ALL-111584", "label": "XMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57464", "formula": "C10H11N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113578", "reactome_id": "R-ALL-113578", "label": "GMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58115", "formula": "C10H12N5O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "GMP synthase (glutamine-hydrolyzing) activity of GMPS [cytosol]", "activity_id": "73785", "ec_numbers": [ "6.3.5.2" ], "accessions": [ "P49915" ] } ], "citations": [ { "id": "PMID:8089153", "title": "Human GMP synthetase. Protein purification, cloning, and functional expression of cDNA", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8089153/" }, { "id": "PMID:7706277", "title": "Biochemical characterization of human GMP synthetase", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7706277/" } ], "x": 2660, "y": 2812.34375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73794", "label": "IMP + H2O + NAD+ =\u003e XMP + NADH + H+ [IMPDH1,2]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11709" ], "source_url": "https://reactome.org/content/detail/R-HSA-73794", "summary": "IMP + H2O + NAD+ =\u003e XMP + NADH + H+ [IMPDH1,2] is a Reactome release 97 curated reaction event. The source record connects H2O, IMP, NAD+ to H+, NADH, XMP in cytosol and cross-references RHEA:11709.", "equation": "H2O + IMP + NAD+ → H+ + NADH + XMP", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29602", "reactome_id": "R-ALL-29602", "label": "IMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58053", "formula": "C10H11N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111584", "reactome_id": "R-ALL-111584", "label": "XMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57464", "formula": "C10H11N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "IMP dehydrogenase activity of IMPDH tetramers [cytosol]", "activity_id": "73793", "ec_numbers": [ "1.1.1.205" ], "accessions": [] } ], "citations": [ { "id": "PMID:7903306", "title": "Characterization of human type I and type II IMP dehydrogenases", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7903306/" } ], "x": 2381.3333333333335, "y": 2849.71875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73797", "label": "FAICAR =\u003e IMP + H2O", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18447" ], "source_url": "https://reactome.org/content/detail/R-HSA-73797", "summary": "FAICAR =\u003e IMP + H2O is a Reactome release 97 curated reaction event. The source record connects FAICAR to IMP, H2O in cytosol and cross-references RHEA:18447.", "equation": "FAICAR → IMP + H2O", "inputs": [ { "node_id": "R-ALL-111427", "reactome_id": "R-ALL-111427", "label": "FAICAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58467", "formula": "C10H13N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29602", "reactome_id": "R-ALL-29602", "label": "IMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58053", "formula": "C10H11N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "IMP cyclohydrolase activity of ATIC dimer [cytosol]", "activity_id": "73795", "ec_numbers": [ "3.5.4.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:8567683", "title": "The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8567683/" }, { "id": "PMID:11096114", "title": "Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activity", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11096114/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2938.666666666667, "y": 2887.09375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73798", "label": "AICAR + 10-Formyl-THF =\u003e FAICAR + THF", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22193" ], "source_url": "https://reactome.org/content/detail/R-HSA-73798", "summary": "AICAR + 10-Formyl-THF =\u003e FAICAR + THF is a Reactome release 97 curated reaction event. The source record connects AICAR, 10-formyl-THF to FAICAR, THF in cytosol and cross-references RHEA:22193.", "equation": "AICAR + 10-formyl-THF → FAICAR + THF", "inputs": [ { "node_id": "R-ALL-111409", "reactome_id": "R-ALL-111409", "label": "AICAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58475", "formula": "C9H13N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-419151", "reactome_id": "R-ALL-419151", "label": "10-formyl-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195366", "formula": "C20H21N7O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111427", "reactome_id": "R-ALL-111427", "label": "FAICAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58467", "formula": "C10H13N4O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111355", "reactome_id": "R-ALL-111355", "label": "THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphoribosylaminoimidazolecarboxamide formyltransferase activity of ATIC dimer [cytosol]", "activity_id": "73796", "ec_numbers": [ "2.1.2.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:8567683", "title": "The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8567683/" }, { "id": "PMID:11096114", "title": "Human 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activity", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11096114/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2381.3333333333335, "y": 2924.46875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73800", "label": "SAICAR =\u003e AICAR + Fumarate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23921" ], "source_url": "https://reactome.org/content/detail/R-HSA-73800", "summary": "SAICAR =\u003e AICAR + Fumarate is a Reactome release 97 curated reaction event. The source record connects SAICAR to AICAR, FUMA in cytosol and cross-references RHEA:23921.", "equation": "SAICAR → AICAR + FUMA", "inputs": [ { "node_id": "R-ALL-111399", "reactome_id": "R-ALL-111399", "label": "SAICAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58443", "formula": "C13H15N4O12P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111409", "reactome_id": "R-ALL-111409", "label": "AICAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58475", "formula": "C9H13N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29586", "reactome_id": "R-ALL-29586", "label": "FUMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29806", "formula": "C4H2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity of ADSL tetramer [cytosol]", "activity_id": "73799", "ec_numbers": [ "4.3.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8366112", "title": "Expression, purification, and kinetic characterization of recombinant human adenylosuccinate lyase", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8366112/" }, { "id": "PMID:10958654", "title": "Clinical, biochemical and molecular genetic correlations in adenylosuccinate lyase deficiency", "journal": "Hum Mol Genet", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10958654/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2381.3333333333335, "y": 2905.78125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73805", "label": "CAIR + Aspartate + ATP =\u003e SAICAR + ADP + Pi", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22629" ], "source_url": "https://reactome.org/content/detail/R-HSA-73805", "summary": "CAIR + Aspartate + ATP =\u003e SAICAR + ADP + Pi is a Reactome release 97 curated reaction event. The source record connects CAIR, L-Asp, ATP to SAICAR, ADP, Pi in cytosol and cross-references RHEA:22629.", "equation": "CAIR + L-Asp + ATP → SAICAR + ADP + Pi + 2 H+", "inputs": [ { "node_id": "R-ALL-111395", "reactome_id": "R-ALL-111395", "label": "CAIR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77657", "formula": "C9H12N3O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29448", "reactome_id": "R-ALL-29448", "label": "L-Asp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29991", "formula": "C4H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111399", "reactome_id": "R-ALL-111399", "label": "SAICAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58443", "formula": "C13H15N4O12P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoribosylaminoimidazolesuccinocarboxamide synthase activity of PAICS octamer [cytosol]", "activity_id": "73801", "ec_numbers": [ "6.3.2.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:2183217", "title": "Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations", "journal": "Proc Natl Acad Sci USA", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2183217/" }, { "id": "PMID:17224163", "title": "Octameric structure of the human bifunctional enzyme PAICS in purine biosynthesis", "journal": "J Mol Biol", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17224163/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2660, "y": 2831.03125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73806", "label": "AIR + CO2 =\u003e CAIR", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10794" ], "source_url": "https://reactome.org/content/detail/R-HSA-73806", "summary": "AIR + CO2 =\u003e CAIR is a Reactome release 97 curated reaction event. The source record connects AIR, CO2 to CAIR, H+ in cytosol and cross-references RHEA:10794.", "equation": "AIR + CO2 → CAIR + H+", "inputs": [ { "node_id": "R-ALL-111370", "reactome_id": "R-ALL-111370", "label": "AIR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:137981", "formula": "C8H13N3O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111395", "reactome_id": "R-ALL-111395", "label": "CAIR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:77657", "formula": "C9H12N3O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoribosylaminoimidazole carboxylase activity of PAICS octamer [cytosol]", "activity_id": "73802", "ec_numbers": [ "4.1.1.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:2183217", "title": "Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations", "journal": "Proc Natl Acad Sci USA", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2183217/" }, { "id": "PMID:17224163", "title": "Octameric structure of the human bifunctional enzyme PAICS in purine biosynthesis", "journal": "J Mol Biol", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17224163/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2660, "y": 2887.09375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73810", "label": "FGAM + ATP =\u003e AIR + ADP + Pi", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23033" ], "source_url": "https://reactome.org/content/detail/R-HSA-73810", "summary": "FGAM + ATP =\u003e AIR + ADP + Pi is a Reactome release 97 curated reaction event. The source record connects FGAM, ATP to AIR, ADP, Pi in cytosol and cross-references RHEA:23033.", "equation": "FGAM + ATP → AIR + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-111369", "reactome_id": "R-ALL-111369", "label": "FGAM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:147287", "formula": "C8H15N3O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111370", "reactome_id": "R-ALL-111370", "label": "AIR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:137981", "formula": "C8H13N3O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoribosylformylglycinamidine cyclo-ligase activity of GART [cytosol]", "activity_id": "73809", "ec_numbers": [ "6.3.3.1" ], "accessions": [ "P22102" ] } ], "citations": [ { "id": "PMID:2147474", "title": "De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli", "journal": "Nucleic Acids Res", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2147474/" }, { "id": "PMID:11381136", "title": "Localization of GAR transformylase in Escherichia coli and mammalian cells", "journal": "Proc Natl Acad Sci USA", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11381136/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2660, "y": 2849.71875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73812", "label": "FGAR + L-Glutamine + ATP + H2O =\u003e FGAM + L-Glutamate + ADP + Pi", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17130" ], "source_url": "https://reactome.org/content/detail/R-HSA-73812", "summary": "FGAR + L-Glutamine + ATP + H2O =\u003e FGAM + L-Glutamate + ADP + Pi is a Reactome release 97 curated reaction event. The source record connects N-Formyl-GAR, L-Gln, ATP to FGAM, L-Glu, ADP in cytosol and cross-references RHEA:17130.", "equation": "N-Formyl-GAR + L-Gln + ATP + H2O → FGAM + L-Glu + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-111354", "reactome_id": "R-ALL-111354", "label": "N-Formyl-GAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:147286", "formula": "C8H13N2O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111369", "reactome_id": "R-ALL-111369", "label": "FGAM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:147287", "formula": "C8H15N3O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoribosylformylglycinamidine synthase activity of PFAS [cytosol]", "activity_id": "73811", "ec_numbers": [ "6.3.5.3" ], "accessions": [ "O15067" ] } ], "citations": [ { "id": "PMID:8110788", "title": "Purification of, generation of monoclonal antibodies to, and mapping of phosphoribosyl N-formylglycinamide amidotransferase", "journal": "Biochemistry", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8110788/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2938.666666666667, "y": 2812.34375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73813", "label": "GAR + 10-Formyl-THF =\u003e FGAR + THF", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-73813", "summary": "GAR + 10-Formyl-THF =\u003e FGAR + THF is a Reactome release 97 curated reaction event. The source record connects PRGA, 10-formyl-THF to N-Formyl-GAR, THF, H+ in cytosol.", "equation": "PRGA + 10-formyl-THF → N-Formyl-GAR + THF + H+", "inputs": [ { "node_id": "R-ALL-111338", "reactome_id": "R-ALL-111338", "label": "PRGA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:143788", "formula": "C7H14N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-419151", "reactome_id": "R-ALL-419151", "label": "10-formyl-THF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195366", "formula": "C20H21N7O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-111354", "reactome_id": "R-ALL-111354", "label": "N-Formyl-GAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:147286", "formula": "C8H13N2O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111355", "reactome_id": "R-ALL-111355", "label": "THF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57453", "formula": "C19H21N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoribosylglycinamide formyltransferase activity of GART [cytosol]", "activity_id": "73808", "ec_numbers": [ "2.1.2.2" ], "accessions": [ "P22102" ] } ], "citations": [ { "id": "PMID:2147474", "title": "De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli", "journal": "Nucleic Acids Res", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2147474/" }, { "id": "PMID:12450384", "title": "Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR", "journal": "Biochemistry", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12450384/" }, { "id": "PMID:11381136", "title": "Localization of GAR transformylase in Escherichia coli and mammalian cells", "journal": "Proc Natl Acad Sci USA", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11381136/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2660, "y": 2924.46875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73814", "label": "5-Phosphoribosylamine + Glycine + ATP =\u003e GAR + ADP + Pi", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17454" ], "source_url": "https://reactome.org/content/detail/R-HSA-73814", "summary": "5-Phosphoribosylamine + Glycine + ATP =\u003e GAR + ADP + Pi is a Reactome release 97 curated reaction event. The source record connects PRA, Gly, ATP to PRGA, ADP, Pi in cytosol and cross-references RHEA:17454.", "equation": "PRA + Gly + ATP → PRGA + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-111292", "reactome_id": "R-ALL-111292", "label": "PRA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58681", "formula": "C5H11NO7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111338", "reactome_id": "R-ALL-111338", "label": "PRGA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:143788", "formula": "C7H14N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoribosylamine-glycine ligase activity of GART [cytosol]", "activity_id": "73807", "ec_numbers": [ "6.3.4.13" ], "accessions": [ "P22102" ] } ], "citations": [ { "id": "PMID:2147474", "title": "De novo purine nucleotide biosynthesis: cloning of human and avian cDNAs encoding the trifunctional glycinamide ribonucleotide synthetase-aminoimidazole ribonucleotide synthetase-glycinamide ribonucleotide transformylase by functional complementation in E. coli", "journal": "Nucleic Acids Res", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2147474/" }, { "id": "PMID:11381136", "title": "Localization of GAR transformylase in Escherichia coli and mammalian cells", "journal": "Proc Natl Acad Sci USA", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11381136/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2938.666666666667, "y": 2831.03125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73815", "label": "5-phospho-alpha-D-ribose 1-diphosphate (PRPP) + H2O + L-glutamine 5-phosphoribosylamine + L-glutamate +pyrophosphate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14907" ], "source_url": "https://reactome.org/content/detail/R-HSA-73815", "summary": "5-phospho-alpha-D-ribose 1-diphosphate (PRPP) + H2O + L-glutamine 5-phosphoribosylamine + L-glutamate +pyrophosphate is a Reactome release 97 curated reaction event. The source record connects PRPP, L-Gln, H2O to PRA, L-Glu, PPi in cytosol and cross-references RHEA:14907.", "equation": "PRPP + L-Gln + H2O → PRA + L-Glu + PPi", "inputs": [ { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-111292", "reactome_id": "R-ALL-111292", "label": "PRA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58681", "formula": "C5H11NO7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "amidophosphoribosyltransferase activity of PPAT dimer [cytosol]", "activity_id": "73791", "ec_numbers": [ "2.4.2.14" ], "accessions": [] } ], "citations": [ { "id": "PMID:4348202", "title": "Human glutamine phosphoribosylpyrophosphate amidotransferase. Kinetic and regulatory properties", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4348202/" }, { "id": "PMID:4726295", "title": "Human glutamine phosphoribosylpyrophosphate amidotransferase. Two molecular forms interconvertible by purine ribonucleotides and phosphoribosylpyrophosphate", "journal": "J Biol Chem", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4726295/" }, { "id": "PMID:762062", "title": "Human amidophosphoribosyltransferase. An oxygen-sensitive iron-sulfur protein", "journal": "J Biol Chem", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/762062/" }, { "id": "PMID:8380692", "title": "Molecular cloning of human amidophosphoribosyltransferase", "journal": "Biochem Biophys Res Commun", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8380692/" }, { "id": "PMID:18388293", "title": "Reversible compartmentalization of de novo purine biosynthetic complexes in living cells", "journal": "Science", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18388293/" } ], "x": 2938.666666666667, "y": 2849.71875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73828", "label": "adenylosuccinate =\u003e adenosine 5'-monophosphate + fumarate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16854" ], "source_url": "https://reactome.org/content/detail/R-HSA-73828", "summary": "adenylosuccinate =\u003e adenosine 5'-monophosphate + fumarate is a Reactome release 97 curated reaction event. The source record connects ADS to AMP, FUMA in cytosol and cross-references RHEA:16854.", "equation": "ADS → AMP + FUMA", "inputs": [ { "node_id": "R-ALL-35141", "reactome_id": "R-ALL-35141", "label": "ADS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57567", "formula": "C14H14N5O11P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29586", "reactome_id": "R-ALL-29586", "label": "FUMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29806", "formula": "C4H2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity of ADSL tetramer [cytosol]", "activity_id": "73799", "ec_numbers": [ "4.3.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:8366112", "title": "Expression, purification, and kinetic characterization of recombinant human adenylosuccinate lyase", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8366112/" } ], "x": 2660, "y": 2905.78125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73912", "label": "acetoacetic acid + NADH + H+ beta-hydroxybutyrate + NAD+", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:20523" ], "source_url": "https://reactome.org/content/detail/R-HSA-73912", "summary": "acetoacetic acid + NADH + H+ beta-hydroxybutyrate + NAD+ is a Reactome release 97 curated reaction event. The source record connects ACA, NADH, H+ to bHBA, NAD+ in mitochondrial matrix and cross-references RHEA:20523.", "equation": "ACA + NADH + H+ → bHBA + NAD+", "inputs": [ { "node_id": "R-ALL-73909", "reactome_id": "R-ALL-73909", "label": "ACA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15344", "formula": "C4H6O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73911", "reactome_id": "R-ALL-73911", "label": "bHBA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17066", "formula": "C4H8O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-hydroxybutyrate dehydrogenase activity of BDH1 tetramer [mitochondrial matrix]", "activity_id": "74155", "ec_numbers": [ "1.1.1.30" ], "accessions": [] } ], "citations": [ { "id": "PMID:1639787", "title": "Molecular cloning and characterization of (R)-3-hydroxybutyrate dehydrogenase from human heart", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1639787/" } ], "x": 7461.333333333333, "y": 408.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73916", "label": "2 acetyl-CoA acetoacetyl-CoA + CoA", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:21037" ], "source_url": "https://reactome.org/content/detail/R-HSA-73916", "summary": "2 acetyl-CoA acetoacetyl-CoA + CoA is a Reactome release 97 curated reaction event. The source record connects Ac-CoA to ACA-CoA, CoA-SH in mitochondrial matrix and cross-references RHEA:21037.", "equation": "2 Ac-CoA → ACA-CoA + CoA-SH", "inputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73871", "reactome_id": "R-ALL-73871", "label": "ACA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57286", "formula": "C25H36N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acetyl-CoA C-acetyltransferase activity of ACAT1 tetramer [mitochondrial matrix]", "activity_id": "70842", "ec_numbers": [ "2.3.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:3709573", "title": "3-Ketothiolase deficiency", "journal": "Eur J Pediatr", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3709573/" } ], "x": 8018.666666666667, "y": 506.125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73918", "label": "acetoacetyl-CoA+acetyl-CoA =\u003e HMG-CoA + CoASH", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:10189" ], "source_url": "https://reactome.org/content/detail/R-HSA-73918", "summary": "acetoacetyl-CoA+acetyl-CoA =\u003e HMG-CoA + CoASH is a Reactome release 97 curated reaction event. The source record connects ACA-CoA, Ac-CoA to HMG CoA, CoA-SH in mitochondrial matrix and cross-references RHEA:10189.", "equation": "ACA-CoA + Ac-CoA → HMG CoA + CoA-SH", "inputs": [ { "node_id": "R-ALL-73871", "reactome_id": "R-ALL-73871", "label": "ACA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57286", "formula": "C25H36N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73917", "reactome_id": "R-ALL-73917", "label": "HMG CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15467", "formula": "C27H44N7O20P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "hydroxymethylglutaryl-CoA synthase activity of HMGCS2 [mitochondrial matrix]", "activity_id": "74170", "ec_numbers": [ "2.3.3.10" ], "accessions": [ "P54868" ] } ], "citations": [ { "id": "PMID:11479731", "title": "Genetic basis of mitochondrial HMG-CoA synthase deficiency", "journal": "Hum Genet", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11479731/" } ], "x": 8018.666666666667, "y": 408.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-73920", "label": "D-beta hydroxybutyrate+NAD+ acetoacetate+NADH+H+", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:20522" ], "source_url": "https://reactome.org/content/detail/R-HSA-73920", "summary": "D-beta hydroxybutyrate+NAD+ acetoacetate+NADH+H+ is a Reactome release 97 curated reaction event. The source record connects bHBA, NAD+ to ACA, NADH, H+ in mitochondrial matrix and cross-references RHEA:20522.", "equation": "bHBA + NAD+ → ACA + NADH + H+", "inputs": [ { "node_id": "R-ALL-73911", "reactome_id": "R-ALL-73911", "label": "bHBA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17066", "formula": "C4H8O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73909", "reactome_id": "R-ALL-73909", "label": "ACA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15344", "formula": "C4H6O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-hydroxybutyrate dehydrogenase activity of BDH1 tetramer [mitochondrial matrix]", "activity_id": "74155", "ec_numbers": [ "1.1.1.30" ], "accessions": [] } ], "citations": [ { "id": "PMID:1639787", "title": "Molecular cloning and characterization of (R)-3-hydroxybutyrate dehydrogenase from human heart", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1639787/" } ], "x": 7740, "y": 408.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-741449", "label": "SLC35B2,3 exchange cytosolic PAPS with Golgi PAP", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen · cytosol", "compartment_ids": [ "GO:0000139", "GO:0005796", "GO:0005829" ], "rhea_ids": [ "RHEA:76065", "RHEA:76063" ], "source_url": "https://reactome.org/content/detail/R-HSA-741449", "summary": "SLC35B2,3 exchange cytosolic PAPS with Golgi PAP is a Reactome release 97 curated reaction event. The source record connects PAPS, PAP to PAPS, PAP in Golgi membrane · Golgi lumen · cytosol and cross-references RHEA:76065, RHEA:76063.", "equation": "PAPS + PAP → PAPS + PAP", "inputs": [ { "node_id": "R-ALL-158471", "reactome_id": "R-ALL-158471", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158466", "reactome_id": "R-ALL-158466", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3'-phosphoadenosine 5'-phosphosulfate transmembrane transporter activity of SLC35B2,3 [Golgi membrane]", "activity_id": "741439", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8619989", "title": "Identification and partial purification of PAPS translocase", "journal": "Biochemistry", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8619989/" }, { "id": "PMID:12716889", "title": "Molecular cloning and identification of 3'-phosphoadenosine 5'-phosphosulfate transporter", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12716889/" }, { "id": "PMID:16492677", "title": "Molecular cloning and characterization of a novel 3'-phosphoadenosine 5'-phosphosulfate transporter, PAPST2", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16492677/" }, { "id": "PMID:35325049", "title": "Biallelic variants in SLC35B2 cause a novel chondrodysplasia with hypomyelinating leukodystrophy", "journal": "Brain", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35325049/" } ], "x": 2381.3333333333335, "y": 759.6818181818181, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74177", "label": "OXCT dimers transfer CoA from SUCC-CoA to ACA, forming ACA-CoA", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:25481" ], "source_url": "https://reactome.org/content/detail/R-HSA-74177", "summary": "OXCT dimers transfer CoA from SUCC-CoA to ACA, forming ACA-CoA is a Reactome release 97 curated reaction event. The source record connects ACA, SUCC-CoA to ACA-CoA, SUCCA in mitochondrial matrix and cross-references RHEA:25481.", "equation": "ACA + SUCC-CoA → ACA-CoA + SUCCA", "inputs": [ { "node_id": "R-ALL-73909", "reactome_id": "R-ALL-73909", "label": "ACA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15344", "formula": "C4H6O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70958", "reactome_id": "R-ALL-70958", "label": "SUCC-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57292", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-73871", "reactome_id": "R-ALL-73871", "label": "ACA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57286", "formula": "C25H36N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113536", "reactome_id": "R-ALL-113536", "label": "SUCCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30031", "formula": "C4H4O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "succinyl-CoA:3-oxo-acid CoA-transferase activity of OXCT dimers [mitochondrial matrix]", "activity_id": "74278", "ec_numbers": [ "2.8.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:8751852", "title": "Succinyl CoA: 3-oxoacid CoA transferase (SCOT): human cDNA cloning, human chromosomal mapping to 5p13, and mutation detection in a SCOT-deficient patient", "journal": "Am J Hum Genet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8751852/" }, { "id": "PMID:11756565", "title": "Cloning and characterization of a human orthologue of testis-specific succinyl CoA: 3-oxo acid CoA transferase (Scot-t) cDNA", "journal": "Mol. Hum. Reprod.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11756565/" } ], "x": 7461.333333333333, "y": 506.125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74180", "label": "HMG CoA =\u003e acetoacetic acid + acetyl CoA", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24405" ], "source_url": "https://reactome.org/content/detail/R-HSA-74180", "summary": "HMG CoA =\u003e acetoacetic acid + acetyl CoA is a Reactome release 97 curated reaction event. The source record connects HMG CoA to Ac-CoA, ACA in mitochondrial matrix and cross-references RHEA:24405.", "equation": "HMG CoA → Ac-CoA + ACA", "inputs": [ { "node_id": "R-ALL-73917", "reactome_id": "R-ALL-73917", "label": "HMG CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15467", "formula": "C27H44N7O20P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73909", "reactome_id": "R-ALL-73909", "label": "ACA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15344", "formula": "C4H6O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydroxymethylglutaryl-CoA lyase activity of HMGCL dimer [mitochondrial matrix]", "activity_id": "74179", "ec_numbers": [ "4.1.3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:8440722", "title": "3-Hydroxy-3-methylglutaryl coenzyme A lyase (HL). Cloning of human and chicken liver HL cDNAs and characterization of a mutation causing human HL deficiency.", "journal": "J Biol Chem", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8440722/" } ], "x": 7461.333333333333, "y": 603.375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74181", "label": "acetoacetyl-CoA + CoA 2 acetyl-CoA", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:21038" ], "source_url": "https://reactome.org/content/detail/R-HSA-74181", "summary": "acetoacetyl-CoA + CoA 2 acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects ACA-CoA, CoA-SH to Ac-CoA in mitochondrial matrix and cross-references RHEA:21038.", "equation": "ACA-CoA + CoA-SH → 2 Ac-CoA", "inputs": [ { "node_id": "R-ALL-73871", "reactome_id": "R-ALL-73871", "label": "ACA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57286", "formula": "C25H36N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acetyltransferase activity of ACAT1 tetramer [mitochondrial matrix]", "activity_id": "70842", "ec_numbers": [ "2.3.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:3709573", "title": "3-Ketothiolase deficiency", "journal": "Eur J Pediatr", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3709573/" } ], "x": 7740, "y": 603.375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74207", "label": "dA, dG, or dI + ATP =\u003e dAMP, dGMP, or dIMP + ADP (DGUOK)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:23453", "RHEA:19202" ], "source_url": "https://reactome.org/content/detail/R-HSA-74207", "summary": "dA, dG, or dI + ATP =\u003e dAMP, dGMP, or dIMP + ADP (DGUOK) is a Reactome release 97 curated reaction event. The source record connects dA, dG, dI, ATP to dAMP, dGMP, dIMP, ADP, H+ in mitochondrial matrix and cross-references RHEA:23453, RHEA:19202.", "equation": "dA, dG, dI + ATP → dAMP, dGMP, dIMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-500224", "reactome_id": "R-ALL-500224", "label": "dA, dG, dI", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500222", "reactome_id": "R-ALL-500222", "label": "dAMP, dGMP, dIMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "deoxynucleoside kinase activity of DGUOK dimer [mitochondrial matrix]", "activity_id": "74206", "ec_numbers": [ "2.7.1.145" ], "accessions": [] } ], "citations": [ { "id": "PMID:8692979", "title": "Cloning and expression of human deoxyguanosine kinase cDNA", "journal": "Proc Natl Acad Sci USA", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8692979/" }, { "id": "PMID:2845866", "title": "Properties of a highly purified mitochondrial deoxyguanosine kinase", "journal": "Arch Biochem Biophys", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2845866/" }, { "id": "PMID:11427893", "title": "Structural basis for substrate specificities of cellular deoxyribonucleoside kinases", "journal": "Nat Struct Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11427893/" } ], "x": 3600, "y": 2813.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74213", "label": "APRT catalyzes the conversion of adenine to AMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16611" ], "source_url": "https://reactome.org/content/detail/R-HSA-74213", "summary": "APRT catalyzes the conversion of adenine to AMP is a Reactome release 97 curated reaction event. The source record connects Ade, PRPP to AMP, PPi in cytosol and cross-references RHEA:16611.", "equation": "Ade + PRPP → AMP + PPi", "inputs": [ { "node_id": "R-ALL-83951", "reactome_id": "R-ALL-83951", "label": "Ade", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16708", "formula": "C5H5N5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "adenine phosphoribosyltransferase activity of APRT dimer [cytosol]", "activity_id": "74212", "ec_numbers": [ "2.4.2.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:457664", "title": "Human adenine phosphoribosyltransferase. Affinity purification, subunit structure, amino acid composition, and peptide mapping.", "journal": "J Biol Chem", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/457664/" }, { "id": "PMID:18399692", "title": "Structural complexes of human adenine phosphoribosyltransferase reveal novel features of the APRT catalytic mechanism", "journal": "J Biomol Struct Dyn", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18399692/" } ], "x": 3321.3333333333335, "y": 2877.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74215", "label": "HPRT1 catalyzes the conversion of guanine or hypoxanthine to GMP or IMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25426", "RHEA:17975" ], "source_url": "https://reactome.org/content/detail/R-HSA-74215", "summary": "HPRT1 catalyzes the conversion of guanine or hypoxanthine to GMP or IMP is a Reactome release 97 curated reaction event. The source record connects Gua, Hyp, PRPP to GMP, IMP, PPi in cytosol and cross-references RHEA:25426, RHEA:17975.", "equation": "Gua, Hyp + PRPP → GMP, IMP + PPi", "inputs": [ { "node_id": "R-ALL-500259", "reactome_id": "R-ALL-500259", "label": "Gua, Hyp", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-73566", "reactome_id": "R-ALL-73566", "label": "PRPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58017", "formula": "C5H8O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-500260", "reactome_id": "R-ALL-500260", "label": "GMP, IMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "purine phosphoribosyltransferase activity of HPRT1 tetramer [cytosol]", "activity_id": "74210", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:6300847", "title": "Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase", "journal": "Proc Natl Acad Sci U S A", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6300847/" } ], "x": 3600, "y": 2920.464285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74220", "label": "(d)AMP + ATP (d)ADP + ADP (AK1)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12974", "RHEA:23101" ], "source_url": "https://reactome.org/content/detail/R-HSA-74220", "summary": "(d)AMP + ATP (d)ADP + ADP (AK1) is a Reactome release 97 curated reaction event. The source record connects (d)AMP, ATP to (d)ADP, ADP in cytosol and cross-references RHEA:12974, RHEA:23101.", "equation": "(d)AMP + ATP → (d)ADP + ADP", "inputs": [ { "node_id": "R-ALL-500088", "reactome_id": "R-ALL-500088", "label": "(d)AMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500087", "reactome_id": "R-ALL-500087", "label": "(d)ADP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "AMP kinase activity of Adenylate Kinase [cytosol]", "activity_id": "73829", "ec_numbers": [ "2.7.4.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:211388", "title": "AMP (dAMP) kinase from human erythrocytes", "journal": "Methods Enzymol", "year": 1978, "url": "https://pubmed.ncbi.nlm.nih.gov/211388/" }, { "id": "PMID:2542324", "title": "Human adenylate kinase deficiency associated with hemolytic anemia. A single base substitution affecting solubility and catalytic activity of the cytosolic adenylate kinase.", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2542324/" } ], "x": 3600, "y": 3098.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74241", "label": "ADA catalyzes the deamination of (deoxy)adenosine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:28191", "RHEA:24409" ], "source_url": "https://reactome.org/content/detail/R-HSA-74241", "summary": "ADA catalyzes the deamination of (deoxy)adenosine is a Reactome release 97 curated reaction event. The source record connects Ade-Rib, dA, H2O, H+ to Ino, dI, NH4+ in cytosol and cross-references RHEA:28191, RHEA:24409.", "equation": "Ade-Rib, dA + H2O + H+ → Ino, dI + NH4+", "inputs": [ { "node_id": "R-ALL-500173", "reactome_id": "R-ALL-500173", "label": "Ade-Rib, dA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500172", "reactome_id": "R-ALL-500172", "label": "Ino, dI", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "deaminase activity of ADA [cytosol]", "activity_id": "74234", "ec_numbers": null, "accessions": [ "P00813" ] } ], "citations": [ { "id": "PMID:3182793", "title": "Mutant human adenosine deaminase alleles and their expression by transfection into fibroblasts", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3182793/" } ], "x": 3878.666666666667, "y": 2813.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74242", "label": "PNP catalyzes the conversion of (deoxy)inosine to hypoxanthine and (deoxy)ribose", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27647", "RHEA:27751" ], "source_url": "https://reactome.org/content/detail/R-HSA-74242", "summary": "PNP catalyzes the conversion of (deoxy)inosine to hypoxanthine and (deoxy)ribose is a Reactome release 97 curated reaction event. The source record connects Pi, Ino, dI to Hyp, R1P, dRibP in cytosol and cross-references RHEA:27647, RHEA:27751.", "equation": "Pi + Ino, dI → Hyp + R1P, dRibP", "inputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500172", "reactome_id": "R-ALL-500172", "label": "Ino, dI", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113599", "reactome_id": "R-ALL-113599", "label": "Hyp", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17368", "formula": "C5H4N4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500244", "reactome_id": "R-ALL-500244", "label": "R1P, dRibP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "purine-nucleoside phosphorylase activity of PNP trimer [cytosol]", "activity_id": "74238", "ec_numbers": [ "2.4.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6771276", "title": "Characterization of purine nucleoside phosphorylase from human granulocytes and its metabolism of deoxyribonucleosides", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6771276/" }, { "id": "PMID:3029074", "title": "A human purine nucleoside phosphorylase deficiency caused by a single base change", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3029074/" }, { "id": "PMID:2104852", "title": "Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 A resolution", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2104852/" } ], "x": 2381.3333333333335, "y": 3081.654761904762, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74247", "label": "XDH oxidizes hypoxanthine to form xanthine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:68013" ], "source_url": "https://reactome.org/content/detail/R-HSA-74247", "summary": "XDH oxidizes hypoxanthine to form xanthine is a Reactome release 97 curated reaction event. The source record connects Hyp, H2O, O2 to XAN, H2O2 in cytosol and cross-references RHEA:68013.", "equation": "Hyp + H2O + O2 → XAN + H2O2", "inputs": [ { "node_id": "R-ALL-113599", "reactome_id": "R-ALL-113599", "label": "Hyp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17368", "formula": "C5H4N4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30064", "reactome_id": "R-ALL-30064", "label": "XAN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17712", "formula": "C5H4N4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hypoxanthine oxidase activity of XDH dimer [cytosol]", "activity_id": "9727340", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:23203137", "title": "Mutations associated with functional disorder of xanthine oxidoreductase and hereditary xanthinuria in humans", "journal": "Int J Mol Sci", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23203137/" }, { "id": "PMID:8670112", "title": "Cloning and expression in vitro of human xanthine dehydrogenase/oxidase", "journal": "Biochem J", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8670112/" }, { "id": "PMID:17301077", "title": "Human xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site: roles of active site residues in binding and activation of purine substrate", "journal": "J Biochem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17301077/" } ], "x": 2660, "y": 3131.4880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74248", "label": "(d)GMP or (d)IMP + H2O =\u003e (2'-deoxy)guanosine or (2'-deoxy)inosine + orthophosphate (NT5C2)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27715", "RHEA:29380", "RHEA:27719", "RHEA:29384" ], "source_url": "https://reactome.org/content/detail/R-HSA-74248", "summary": "(d)GMP or (d)IMP + H2O =\u003e (2'-deoxy)guanosine or (2'-deoxy)inosine + orthophosphate (NT5C2) is a Reactome release 97 curated reaction event. The source record connects (d)GMP, (d)IMP, H2O to Gu-Rib, dG, Ino, dI, Pi in cytosol and cross-references RHEA:27715, RHEA:29380, RHEA:27719, RHEA:29384.", "equation": "(d)GMP, (d)IMP + H2O → Gu-Rib, dG, Ino, dI + Pi", "inputs": [ { "node_id": "R-ALL-500120", "reactome_id": "R-ALL-500120", "label": "(d)GMP, (d)IMP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500118", "reactome_id": "R-ALL-500118", "label": "Gu-Rib, dG, Ino, dI", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5C2 tetramer [cytosol]", "activity_id": "109324", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:2848805", "title": "High Km soluble 5'-nucleotidase from human placenta", "journal": "J Biol Chem", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2848805/" }, { "id": "PMID:17405878", "title": "Crystal structure of human cytosolic 5'-nucleotidase II: insights into allosteric regulation and substrate recognition", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17405878/" }, { "id": "PMID:11083867", "title": "Cytosolic high Km 5'-nucleotidase and 5'(3')-deoxyribonucleotidase in substrate cycles involved in nucleotide metabolism", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11083867/" }, { "id": "PMID:10766785", "title": "Distinct roles for recombinant cytosolic 5'-nucleotidase I and -II in AMP and IMP catabolism in COS-7 and H9c2 rat myoblast cell lines", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10766785/" }, { "id": "PMID:12947102", "title": "Mammalian 5'-nucleotidases", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12947102/" } ], "x": 2381.3333333333335, "y": 3067.4166666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74249", "label": "PNP catalyzes the conversion of (deoxy)guanosine to guanine and (deoxy)ribose", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13234", "RHEA:27739" ], "source_url": "https://reactome.org/content/detail/R-HSA-74249", "summary": "PNP catalyzes the conversion of (deoxy)guanosine to guanine and (deoxy)ribose is a Reactome release 97 curated reaction event. The source record connects Pi, G, dG to Gua, R1P, dRibP in cytosol and cross-references RHEA:13234, RHEA:27739.", "equation": "Pi + G, dG → Gua + R1P, dRibP", "inputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500245", "reactome_id": "R-ALL-500245", "label": "G, dG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-83956", "reactome_id": "R-ALL-83956", "label": "Gua", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16235", "formula": "C5H5N5O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500244", "reactome_id": "R-ALL-500244", "label": "R1P, dRibP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "purine-nucleoside phosphorylase activity of PNP trimer [cytosol]", "activity_id": "74238", "ec_numbers": [ "2.4.2.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:6771276", "title": "Characterization of purine nucleoside phosphorylase from human granulocytes and its metabolism of deoxyribonucleosides", "journal": "J Biol Chem", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/6771276/" }, { "id": "PMID:3029074", "title": "A human purine nucleoside phosphorylase deficiency caused by a single base change", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3029074/" }, { "id": "PMID:2104852", "title": "Three-dimensional structure of human erythrocytic purine nucleoside phosphorylase at 3.2 A resolution", "journal": "J Biol Chem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2104852/" } ], "x": 2660, "y": 3174.2023809523807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74255", "label": "Guanine + H2O =\u003e Xanthine + NH4+", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14666" ], "source_url": "https://reactome.org/content/detail/R-HSA-74255", "summary": "Guanine + H2O =\u003e Xanthine + NH4+ is a Reactome release 97 curated reaction event. The source record connects Gua, H2O, H+ to XAN, NH4+ in cytosol and cross-references RHEA:14666.", "equation": "Gua + H2O + H+ → XAN + NH4+", "inputs": [ { "node_id": "R-ALL-83956", "reactome_id": "R-ALL-83956", "label": "Gua", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16235", "formula": "C5H5N5O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30064", "reactome_id": "R-ALL-30064", "label": "XAN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17712", "formula": "C5H4N4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "guanine deaminase activity of GDA dimer [cytosol]", "activity_id": "74254", "ec_numbers": [ "3.5.4.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:10075721", "title": "Cloning and characterization of human guanine deaminase. Purification and partial amino acid sequence of the mouse protein.", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10075721/" }, { "id": "PMID:19470646", "title": "Alteration of enzyme specificity by computational loop remodeling and design", "journal": "Proc Natl Acad Sci U S A", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19470646/" } ], "x": 2938.666666666667, "y": 3131.4880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74258", "label": "XDH oxidizes xanthine to form urate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21133" ], "source_url": "https://reactome.org/content/detail/R-HSA-74258", "summary": "XDH oxidizes xanthine to form urate is a Reactome release 97 curated reaction event. The source record connects XAN, H2O, O2 to urate, H2O2 in cytosol and cross-references RHEA:21133.", "equation": "XAN + H2O + O2 → urate + H2O2", "inputs": [ { "node_id": "R-ALL-30064", "reactome_id": "R-ALL-30064", "label": "XAN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17712", "formula": "C5H4N4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30034", "reactome_id": "R-ALL-30034", "label": "urate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17775", "formula": "C5H4N4O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "xanthine oxidase activity of XDH dimer [cytosol]", "activity_id": "74246", "ec_numbers": [ "1.17.3.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:23203137", "title": "Mutations associated with functional disorder of xanthine oxidoreductase and hereditary xanthinuria in humans", "journal": "Int J Mol Sci", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23203137/" }, { "id": "PMID:8670112", "title": "Cloning and expression in vitro of human xanthine dehydrogenase/oxidase", "journal": "Biochem J", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8670112/" }, { "id": "PMID:17301077", "title": "Human xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site: roles of active site residues in binding and activation of purine substrate", "journal": "J Biochem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17301077/" } ], "x": 2381.3333333333335, "y": 3138.6071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74372", "label": "uracil + (deoxy)ribose 1-phosphate (deoxy)uridine + orthophosphate [UPP]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24390", "RHEA:22826" ], "source_url": "https://reactome.org/content/detail/R-HSA-74372", "summary": "uracil + (deoxy)ribose 1-phosphate (deoxy)uridine + orthophosphate [UPP] is a Reactome release 97 curated reaction event. The source record connects Ura, R1P, dRibP to (d)Ura, Pi in cytosol and cross-references RHEA:24390, RHEA:22826.", "equation": "Ura + R1P, dRibP → (d)Ura + Pi", "inputs": [ { "node_id": "R-ALL-83962", "reactome_id": "R-ALL-83962", "label": "Ura", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17568", "formula": "C4H4N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500244", "reactome_id": "R-ALL-500244", "label": "R1P, dRibP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-500430", "reactome_id": "R-ALL-500430", "label": "(d)Ura", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "uridine phosphorylase activity of UPP1, UPP2 [cytosol]", "activity_id": "73557", "ec_numbers": [ "2.4.2.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:7488099", "title": "Cloning and expression of human uridine phosphorylase", "journal": "Biochem Biophys Res Commun", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7488099/" }, { "id": "PMID:19291308", "title": "Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy", "journal": "BMC Struct Biol", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19291308/" }, { "id": "PMID:12849978", "title": "Identification of a novel human uridine phosphorylase", "journal": "Biochem Biophys Res Commun", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12849978/" } ], "x": 3878.666666666667, "y": 2920.464285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-74376", "label": "(deoxy)uridine + orthophosphate uracil + (deoxy)ribose 1-phosphate (UPP)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24389", "RHEA:22825" ], "source_url": "https://reactome.org/content/detail/R-HSA-74376", "summary": "(deoxy)uridine + orthophosphate uracil + (deoxy)ribose 1-phosphate (UPP) is a Reactome release 97 curated reaction event. The source record connects (d)Ura, Pi to Ura, R1P, dRibP in cytosol and cross-references RHEA:24389, RHEA:22825.", "equation": "(d)Ura + Pi → Ura + R1P, dRibP", "inputs": [ { "node_id": "R-ALL-500430", "reactome_id": "R-ALL-500430", "label": "(d)Ura", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-83962", "reactome_id": "R-ALL-83962", "label": "Ura", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17568", "formula": "C4H4N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-500244", "reactome_id": "R-ALL-500244", "label": "R1P, dRibP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "uridine phosphorylase activity of UPP1, UPP2 [cytosol]", "activity_id": "73557", "ec_numbers": [ "2.4.2.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:7488099", "title": "Cloning and expression of human uridine phosphorylase", "journal": "Biochem Biophys Res Commun", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7488099/" }, { "id": "PMID:19291308", "title": "Implications of the structure of human uridine phosphorylase 1 on the development of novel inhibitors for improving the therapeutic window of fluoropyrimidine chemotherapy", "journal": "BMC Struct Biol", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19291308/" }, { "id": "PMID:12849978", "title": "Identification of a novel human uridine phosphorylase", "journal": "Biochem Biophys Res Commun", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12849978/" } ], "x": 2938.666666666667, "y": 3174.2023809523807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-744231", "label": "SLC35D2 exchanges UDP-GlcNAc for UMP", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane", "compartment_ids": [ "GO:0000139" ], "rhea_ids": [ "RHEA:72695" ], "source_url": "https://reactome.org/content/detail/R-HSA-744231", "summary": "SLC35D2 exchanges UDP-GlcNAc for UMP is a Reactome release 97 curated reaction event. The source record connects UMP, UDP-GlcNAc to UMP, UDP-GlcNAc in Golgi membrane and cross-references RHEA:72695.", "equation": "UMP + UDP-GlcNAc → UMP + UDP-GlcNAc", "inputs": [ { "node_id": "R-ALL-735698", "reactome_id": "R-ALL-735698", "label": "UMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57865", "formula": "C9H11N2O9P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-162756", "reactome_id": "R-ALL-162756", "label": "UDP-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-109402", "reactome_id": "R-ALL-109402", "label": "UMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57865", "formula": "C9H11N2O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-914003", "reactome_id": "R-ALL-914003", "label": "UDP-GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nucleotide-sugar transmembrane transporter activity of SLC35D2 [Golgi membrane]", "activity_id": "744232", "ec_numbers": null, "accessions": [ "Q76EJ3" ] } ], "citations": [ { "id": "PMID:15082721", "title": "Molecular cloning and characterization of a human multisubstrate specific nucleotide-sugar transporter homologous to Drosophila fringe connection", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15082721/" }, { "id": "PMID:15607426", "title": "Identification and characterization of human Golgi nucleotide sugar transporter SLC35D2, a novel member of the SLC35 nucleotide sugar transporter family", "journal": "Genomics", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15607426/" } ], "x": 2660, "y": 759.6818181818181, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75125", "label": "(d)CDP or UDP + ADP (d)CMP or UMP + ATP (CMPK1)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24402", "RHEA:25096", "RHEA:11602" ], "source_url": "https://reactome.org/content/detail/R-HSA-75125", "summary": "(d)CDP or UDP + ADP (d)CMP or UMP + ATP (CMPK1) is a Reactome release 97 curated reaction event. The source record connects (d)CDP, UDP, ADP to (d)CMP, UMP, ATP in cytosol and cross-references RHEA:24402, RHEA:25096, RHEA:11602.", "equation": "(d)CDP, UDP + ADP → (d)CMP, UMP + ATP", "inputs": [ { "node_id": "R-ALL-500006", "reactome_id": "R-ALL-500006", "label": "(d)CDP, UDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500009", "reactome_id": "R-ALL-500009", "label": "(d)CMP, UMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside monophosphate kinase activity of CMPK1, AK1 [cytosol]", "activity_id": "73495", "ec_numbers": [ "2.7.4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:11912132", "title": "Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates.", "journal": "Cancer Res", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11912132/" }, { "id": "PMID:40615", "title": "Kinetics and equilibria of pyrimidine nucleoside monophosphate kinase from human erythrocytes", "journal": "Biochim Biophys Acta", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/40615/" } ], "x": 3878.666666666667, "y": 3098.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75126", "label": "dUDP or TDP + ADP dUMP or TMP + ATP [DTYMK]", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:30657", "RHEA:13519" ], "source_url": "https://reactome.org/content/detail/R-HSA-75126", "summary": "dUDP or TDP + ADP dUMP or TMP + ATP [DTYMK] is a Reactome release 97 curated reaction event. The source record connects dUDP, TDP, ADP to dUMP, TMP, ATP in cytosol and cross-references RHEA:30657, RHEA:13519.", "equation": "dUDP, TDP + ADP → dUMP, TMP + ATP", "inputs": [ { "node_id": "R-ALL-499999", "reactome_id": "R-ALL-499999", "label": "dUDP, TDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-499997", "reactome_id": "R-ALL-499997", "label": "dUMP, TMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside monophosphate kinase activity of DTYMK dimer [cytosol]", "activity_id": "73499", "ec_numbers": [ "2.7.4.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:18469", "title": "Human thymidylate kinase. Purification, characterization, and kinetic behavior of the thymidylate kinase derived from chronic myelocytic leukemia", "journal": "J Biol Chem", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/18469/" } ], "x": 3600, "y": 3148.3958333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75848", "label": "ACLY tetramer transforms CIT to Ac-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21162" ], "source_url": "https://reactome.org/content/detail/R-HSA-75848", "summary": "ACLY tetramer transforms CIT to Ac-CoA is a Reactome release 97 curated reaction event. The source record connects CIT, CoA-SH, ATP to Ac-CoA, ADP, OA in cytosol and cross-references RHEA:21162.", "equation": "CIT + CoA-SH + ATP → Ac-CoA + ADP + OA + Pi", "inputs": [ { "node_id": "R-ALL-76190", "reactome_id": "R-ALL-76190", "label": "CIT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16947", "formula": "C6H5O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76213", "reactome_id": "R-ALL-76213", "label": "OA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ATP citrate synthase activity of ACLY tetramer [cytosol]", "activity_id": "76189", "ec_numbers": [ "2.3.3.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:1371749", "title": "Cloning and expression of a human ATP-citrate lyase cDNA", "journal": "Eur J Biochem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1371749/" }, { "id": "PMID:23932781", "title": "Acetylation stabilizes ATP-citrate lyase to promote lipid biosynthesis and tumor growth", "journal": "Mol. Cell", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23932781/" }, { "id": "PMID:24286864", "title": "Cytosolic functions of MORC2 in lipogenesis and adipogenesis", "journal": "Biochim. Biophys. Acta", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24286864/" }, { "id": "PMID:37051670", "title": "Determiners of cell fates: the tricarboxylic acid cycle versus the citrate-malate shuttle", "journal": "Protein Cell", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37051670/" }, { "id": "PMID:36581208", "title": "Regulation and function of the mammalian tricarboxylic acid cycle", "journal": "J Biol Chem", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36581208/" }, { "id": "PMID:33917812", "title": "Acetyl-CoA Metabolism and Histone Acetylation in the Regulation of Aging and Lifespan", "journal": "Antioxidants (Basel)", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33917812/" } ], "x": 1251.3333333333333, "y": 1647.6666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75851", "label": "Btn-ACACA:2Mn2+ polymer carboxylates Ac-CoA to form Mal-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11309" ], "source_url": "https://reactome.org/content/detail/R-HSA-75851", "summary": "Btn-ACACA:2Mn2+ polymer carboxylates Ac-CoA to form Mal-CoA is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, ATP, HCO3- to ADP, Pi, Mal-CoA in cytosol and cross-references RHEA:11309.", "equation": "Ac-CoA + ATP + HCO3- → ADP + Pi + Mal-CoA + H+", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111627", "reactome_id": "R-ALL-111627", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29508", "reactome_id": "R-ALL-29508", "label": "Mal-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acetyl-CoA carboxylase activity of Btn-ACACA:2Mn2+ polymer [cytosol]", "activity_id": "76186", "ec_numbers": [ "6.4.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:17223360", "title": "Expression, purification, and characterization of human acetyl-CoA carboxylase 2", "journal": "Protein Expr Purif", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17223360/" }, { "id": "PMID:9099716", "title": "Human acetyl-CoA carboxylase 2. Molecular cloning, characterization, chromosomal mapping, and evidence for two isoforms.", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9099716/" }, { "id": "PMID:10677481", "title": "The subcellular localization of acetyl-CoA carboxylase 2", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10677481/" }, { "id": "PMID:20457939", "title": "Induced polymerization of mammalian acetyl-CoA carboxylase by MIG12 provides a tertiary level of regulation of fatty acid synthesis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20457939/" }, { "id": "PMID:22123817", "title": "Selectivity in post-translational biotin addition to five human carboxylases", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22123817/" }, { "id": "PMID:18556348", "title": "Hepatic expression of the SPOT 14 (S14) paralog S14-related (Mid1 interacting protein) is regulated by dietary carbohydrate", "journal": "Endocrinology", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18556348/" } ], "x": 590, "y": 1534.5087719298247, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75872", "label": "Conversion of malonyl-CoA and acetyl-CoA to palmitate", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14994" ], "source_url": "https://reactome.org/content/detail/R-HSA-75872", "summary": "Conversion of malonyl-CoA and acetyl-CoA to palmitate is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, Mal-CoA, NADPH to PALM, CO2, NADP+ in cytosol and cross-references RHEA:14994.", "equation": "Ac-CoA + 7 Mal-CoA + 14 NADPH + 14 H+ → PALM + 7 CO2 + 14 NADP+ + 8 CoA-SH + 6 H2O", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29508", "reactome_id": "R-ALL-29508", "label": "Mal-CoA", "role": "input", "stoichiometry": 7, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 14, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 14, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-3632881", "reactome_id": "R-ALL-3632881", "label": "PALM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:7896", "formula": "C16H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 7, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 14, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 6, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fatty acid synthase activity of FASN dimer [cytosol]", "activity_id": "539119", "ec_numbers": [ "2.3.1.85" ], "accessions": [] } ], "citations": [ { "id": "PMID:7567999", "title": "Human fatty acid synthase: properties and molecular cloning", "journal": "Proc Natl Acad Sci U S A", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7567999/" }, { "id": "PMID:12689621", "title": "Structural and functional organization of the animal fatty acid synthase.", "journal": "Prog Lipid Res", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12689621/" } ], "x": 868.6666666666667, "y": 1640.122807017544, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75885", "label": "1-acyl LPA is acylated to PA by AGPAT (LPAAT)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-75885", "summary": "1-acyl LPA is acylated to PA by AGPAT (LPAAT) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 1-acyl LPA, acyl-CoA to PA, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "1-acyl LPA + acyl-CoA → PA + CoA-SH", "inputs": [ { "node_id": "R-ALL-1500604", "reactome_id": "R-ALL-1500604", "label": "1-acyl LPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426755", "reactome_id": "R-ALL-426755", "label": "PA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1-acylglycerol-3-phosphate O-acyltransferase activity of AGPAT [endoplasmic reticulum membrane]", "activity_id": "1500600", "ec_numbers": [ "2.3.1.51" ], "accessions": [] } ], "citations": [ { "id": "PMID:16620771", "title": "Functional characterization of human 1-acylglycerol-3-phosphate acyltransferase isoform 8: cloning, tissue distribution, gene structure, and enzymatic activity", "journal": "Arch Biochem Biophys", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16620771/" }, { "id": "PMID:9461603", "title": "Characterization of a human lysophosphatidic acid acyltransferase that is encoded by a gene located in the class III region of the human major histocompatibility complex", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9461603/" }, { "id": "PMID:15367102", "title": "Cloning and characterization of murine 1-acyl-sn-glycerol 3-phosphate acyltransferases and their regulation by PPARalpha in murine heart", "journal": "Biochem J", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15367102/" }, { "id": "PMID:18718904", "title": "Acyl-CoA:lysophospholipid acyltransferases", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18718904/" }, { "id": "PMID:19336658", "title": "Biochemistry, physiology, and genetics of GPAT, AGPAT, and lipin enzymes in triglyceride synthesis", "journal": "Am J Physiol Endocrinol Metab", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19336658/" }, { "id": "PMID:9212163", "title": "Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytokine-induced signaling responses in cells", "journal": "DNA Cell Biol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9212163/" } ], "x": 2938.666666666667, "y": 1828.7465753424658, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-75886", "label": "glycerol 3-phosphate + acyl-CoA =\u003e 1-acylglycerol 3-phosphate + CoASH [mitochondrial membrane-associated]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-75886", "summary": "glycerol 3-phosphate + acyl-CoA =\u003e 1-acylglycerol 3-phosphate + CoASH [mitochondrial membrane-associated] is a Reactome release 97 curated reaction event. The source record connects G3P, FACoA to 1-acyl LPA, CoA-SH in cytosol · mitochondrial outer membrane.", "equation": "G3P + FACoA → 1-acyl LPA + CoA-SH", "inputs": [ { "node_id": "R-ALL-76114", "reactome_id": "R-ALL-76114", "label": "G3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-75967", "reactome_id": "R-ALL-75967", "label": "FACoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77636", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76112", "reactome_id": "R-ALL-76112", "label": "1-acyl LPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16975", "formula": "C4H8O7PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycerol-3-phosphate O-acyltransferase activity of GPAM or GPAT2 [mitochondrial outer membrane]", "activity_id": "76113", "ec_numbers": [ "2.3.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:18238778", "title": "AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18238778/" }, { "id": "PMID:18718904", "title": "Acyl-CoA:lysophospholipid acyltransferases", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18718904/" }, { "id": "PMID:19336658", "title": "Biochemistry, physiology, and genetics of GPAT, AGPAT, and lipin enzymes in triglyceride synthesis", "journal": "Am J Physiol Endocrinol Metab", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19336658/" } ], "x": 3321.3333333333335, "y": 2230.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75887", "label": "Conversion of Glycerol to Glycerol-3-phosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21645" ], "source_url": "https://reactome.org/content/detail/R-HSA-75887", "summary": "Conversion of Glycerol to Glycerol-3-phosphate is a Reactome release 97 curated reaction event. The source record connects Glycerol, ATP to G3P, ADP in cytosol and cross-references RHEA:21645.", "equation": "Glycerol + ATP → G3P + ADP", "inputs": [ { "node_id": "R-ALL-76116", "reactome_id": "R-ALL-76116", "label": "Glycerol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17754", "formula": "C3H8O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76114", "reactome_id": "R-ALL-76114", "label": "G3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycerol kinase activity of GK,GK2,GK3P [cytosol]", "activity_id": "76115", "ec_numbers": [ "2.7.1.30" ], "accessions": [] } ], "citations": [ { "id": "PMID:15845384", "title": "Human and murine glycerol kinase: influence of exon 18 alternative splicing on function", "journal": "Biochem Biophys Res Commun", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15845384/" } ], "x": 3878.666666666667, "y": 2133.5625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75889", "label": "DHAP is converted to G3P by GPD1/GPD1L", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11094" ], "source_url": "https://reactome.org/content/detail/R-HSA-75889", "summary": "DHAP is converted to G3P by GPD1/GPD1L is a Reactome release 97 curated reaction event. The source record connects DHAP, NADH, H+ to G3P, NAD+ in cytosol and cross-references RHEA:11094.", "equation": "DHAP + NADH + H+ → G3P + NAD+", "inputs": [ { "node_id": "R-ALL-75970", "reactome_id": "R-ALL-75970", "label": "DHAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57642", "formula": "C3H5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76114", "reactome_id": "R-ALL-76114", "label": "G3P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycerol-3-phosphate dehydrogenase [NAD(P)+] activity of GPD1/GPD1L homodimer [cytosol]", "activity_id": "1500607", "ec_numbers": [ "1.1.1.94" ], "accessions": [] } ], "citations": [ { "id": "PMID:19666841", "title": "GPD1L links redox state to cardiac excitability by PKC-dependent phosphorylation of the sodium channel SCN5A", "journal": "Am J Physiol Heart Circ Physiol", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19666841/" }, { "id": "PMID:16460752", "title": "Crystal structures of human glycerol 3-phosphate dehydrogenase 1 (GPD1)", "journal": "J Mol Biol", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16460752/" } ], "x": 2660, "y": 1540.9931506849316, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75899", "label": "1,2-diacyl-glycerol 3-phosphate + H2O =\u003e 1,2-diacyl-glycerol + orthophosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:27430" ], "source_url": "https://reactome.org/content/detail/R-HSA-75899", "summary": "1,2-diacyl-glycerol 3-phosphate + H2O =\u003e 1,2-diacyl-glycerol + orthophosphate is a Reactome release 97 curated reaction event. The source record connects 1,2-daG3P, H2O to DAG, Pi in cytosol · endoplasmic reticulum membrane and cross-references RHEA:27430.", "equation": "1,2-daG3P + H2O → DAG + Pi", "inputs": [ { "node_id": "R-ALL-76108", "reactome_id": "R-ALL-76108", "label": "1,2-daG3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58608", "formula": "C5H5O8PR2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76103", "reactome_id": "R-ALL-76103", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidate phosphatase activity of LPIN1,2,3 [endoplasmic reticulum membrane]", "activity_id": "549156", "ec_numbers": [ "3.1.3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:17158099", "title": "Three mammalian lipins act as phosphatidate phosphatases with distinct tissue expression patterns", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17158099/" }, { "id": "PMID:18694939", "title": "Temporal and spatial regulation of the phosphatidate phosphatases lipin 1 and 2", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18694939/" } ], "x": 3321.3333333333335, "y": 2182.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-75900", "label": "1,2-diacyl-glycerol + acyl-CoA =\u003e triacylglycerol + CoASH [DGAT1]", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-75900", "summary": "1,2-diacyl-glycerol + acyl-CoA =\u003e triacylglycerol + CoASH [DGAT1] is a Reactome release 97 curated reaction event. The source record connects DAG, FACoA to TAG, CoA-SH in cytosol · endoplasmic reticulum membrane.", "equation": "DAG + FACoA → TAG + CoA-SH", "inputs": [ { "node_id": "R-ALL-76103", "reactome_id": "R-ALL-76103", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-75967", "reactome_id": "R-ALL-75967", "label": "FACoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77636", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-549167", "reactome_id": "R-ALL-549167", "label": "TAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diacylglycerol O-acyltransferase activity of DGAT1 tetramer [endoplasmic reticulum membrane]", "activity_id": "76102", "ec_numbers": [ "2.3.1.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:11672446", "title": "Human acyl-CoA:diacylglycerol acyltransferase is a tetrameric protein", "journal": "Biochem J", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11672446/" } ], "x": 3600, "y": 2230.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76354", "label": "Vinyl chloride is oxidized to 2-Chloroethylene oxide", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76354", "summary": "Vinyl chloride is oxidized to 2-Chloroethylene oxide is a Reactome release 97 curated reaction event. The source record connects VC, NADPH, H+ to H2O, NADP+, 2CEO in smooth endoplasmic reticulum.", "equation": "VC + NADPH + H+ + O2 → H2O + NADP+ + 2CEO", "inputs": [ { "node_id": "R-ALL-39417", "reactome_id": "R-ALL-39417", "label": "VC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28509", "formula": "C2H3Cl", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156524", "reactome_id": "R-ALL-156524", "label": "2CEO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29129", "formula": "C2H3ClO", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2E1 based) [endoplasmic reticulum membrane]", "activity_id": "76353", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:497175", "title": "Activation of vinyl chloride to covalently bound metabolites: roles of 2-chloroethylene oxide and 2-chloroacetaldehyde", "journal": "Biochemistry", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/497175/" }, { "id": "PMID:1664256", "title": "Role of human cytochrome P-450 IIE1 in the oxidation of many low molecular weight cancer suspects", "journal": "Chem Res Toxicol", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1664256/" } ], "x": 7740, "y": 3028.0098039215686, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76373", "label": "N-hydroxylation of 4-aminobiphenyl", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76373", "summary": "N-hydroxylation of 4-aminobiphenyl is a Reactome release 97 curated reaction event. The source record connects ABPH, O2, NADPH to NHABP, H2O, NADP+ in smooth endoplasmic reticulum.", "equation": "ABPH + O2 + NADPH + H+ → NHABP + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-47687", "reactome_id": "R-ALL-47687", "label": "ABPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:1784", "formula": "C12H11N", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-34889", "reactome_id": "R-ALL-34889", "label": "NHABP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16580", "formula": "C12H11NO", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A2 [endoplasmic reticulum membrane]", "activity_id": "76372", "ec_numbers": null, "accessions": [ "P05177" ] } ], "citations": [ { "id": "PMID:9111224", "title": "Metabolism of carcinogenic heterocyclic and aromatic amines by recombinant human cytochrome P450 enzymes", "journal": "Carcinogenesis", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9111224/" }, { "id": "PMID:1934265", "title": "Metabolic activation of carcinogenic heterocyclic aromatic amines by human liver and colon", "journal": "Carcinogenesis", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1934265/" } ], "x": 8018.666666666667, "y": 3028.0098039215686, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76386", "label": "CYP1A2 S-demethylates 6MMP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76386", "summary": "CYP1A2 S-demethylates 6MMP is a Reactome release 97 curated reaction event. The source record connects 6MMP, NADPH, H+ to 6MP, H2O, CH2O in smooth endoplasmic reticulum.", "equation": "6MMP + NADPH + H+ + O2 → 6MP + H2O + CH2O + NADP+", "inputs": [ { "node_id": "R-ALL-76384", "reactome_id": "R-ALL-76384", "label": "6MMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28279", "formula": "C6H6N4S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-33153", "reactome_id": "R-ALL-33153", "label": "6MP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:2208", "formula": "C5H4N4S", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-143501", "reactome_id": "R-ALL-143501", "label": "CH2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16842", "formula": "CH2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A2 [endoplasmic reticulum membrane]", "activity_id": "76372", "ec_numbers": null, "accessions": [ "P05177" ] } ], "citations": [], "x": 7461.333333333333, "y": 2944.107843137255, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76397", "label": "Acetaminophen oxidised to N-acetylbenzoquinoneimine (NAPQI)", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76397", "summary": "Acetaminophen oxidised to N-acetylbenzoquinoneimine (NAPQI) is a Reactome release 97 curated reaction event. The source record connects Acetaminophen (TN TYLENOL), NADP+, H2 to NABQI, H2O, NADPH in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "Acetaminophen (TN TYLENOL) + NADP+ + H2 + O2 → NABQI + H2O + NADPH", "inputs": [ { "node_id": "R-ALL-39427", "reactome_id": "R-ALL-39427", "label": "Acetaminophen (TN TYLENOL)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:46195", "formula": "C8H9NO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113555", "reactome_id": "R-ALL-113555", "label": "H2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18276", "formula": "H2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76396", "reactome_id": "R-ALL-76396", "label": "NABQI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29132", "formula": "C8H7NO2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2E1 based) [endoplasmic reticulum membrane]", "activity_id": "76353", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10741631", "title": "Contribution of CYP2E1 and CYP3A to acetaminophen reactive metabolite formation", "journal": "Clin Pharmacol Ther", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10741631/" } ], "x": 7461.333333333333, "y": 3035.6372549019607, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76416", "label": "Benzene is hydroxylated to phenol", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76416", "summary": "Benzene is hydroxylated to phenol is a Reactome release 97 curated reaction event. The source record connects C6H6, O2, NADPH to PhOH, H2O, NADP+ in smooth endoplasmic reticulum.", "equation": "C6H6 + O2 + NADPH + H+ → PhOH + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-31711", "reactome_id": "R-ALL-31711", "label": "C6H6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16716", "formula": "C6H6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29630", "reactome_id": "R-ALL-29630", "label": "PhOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15882", "formula": "C6H6O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2E1 based) [endoplasmic reticulum membrane]", "activity_id": "76353", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11701230", "title": "Cytochrome P450 isozymes involved in the metabolism of phenol, a benzene metabolite", "journal": "Toxicol Lett", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11701230/" } ], "x": 7740, "y": 3035.6372549019607, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76426", "label": "N-atom dealkylation of caffeine", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76426", "summary": "N-atom dealkylation of caffeine is a Reactome release 97 curated reaction event. The source record connects CAF, O2, NADPH to Paraxanthine, H2O, NADP+ in smooth endoplasmic reticulum.", "equation": "CAF + O2 + NADPH + H+ → Paraxanthine + H2O + NADP+ + CH2O", "inputs": [ { "node_id": "R-ALL-40727", "reactome_id": "R-ALL-40727", "label": "CAF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27732", "formula": "C8H10N4O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76424", "reactome_id": "R-ALL-76424", "label": "Paraxanthine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:25858", "formula": "C7H8N4O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-143501", "reactome_id": "R-ALL-143501", "label": "CH2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16842", "formula": "CH2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A2 [endoplasmic reticulum membrane]", "activity_id": "76372", "ec_numbers": null, "accessions": [ "P05177" ] } ], "citations": [ { "id": "PMID:8553685", "title": "Determination of P4501A2 activity in human liver microsomes using [3-14C-methyl]caffeine", "journal": "Xenobiotica", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8553685/" } ], "x": 7740, "y": 2944.107843137255, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76434", "label": "Dehalogenation of carbon tetrachloride to form a free radical", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76434", "summary": "Dehalogenation of carbon tetrachloride to form a free radical is a Reactome release 97 curated reaction event. The source record connects CCl4 to Trichloromethyl radical, Cl- in smooth endoplasmic reticulum.", "equation": "CCl4 → Trichloromethyl radical + Cl-", "inputs": [ { "node_id": "R-ALL-40887", "reactome_id": "R-ALL-40887", "label": "CCl4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27385", "formula": "CCl4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76433", "reactome_id": "R-ALL-76433", "label": "Trichloromethyl radical", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "OtherEntity", "currency": false }, { "node_id": "R-ALL-29572", "reactome_id": "R-ALL-29572", "label": "Cl-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17996", "formula": "Cl", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2E1 based) [endoplasmic reticulum membrane]", "activity_id": "76353", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10731522", "title": "Cytochrome P450 2E1 is the primary enzyme responsible for low-dose carbon tetrachloride metabolism in human liver microsomes", "journal": "Chem Biol Interact", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10731522/" } ], "x": 7461.333333333333, "y": 3134.794117647059, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76453", "label": "Coumarin is 7-hydroxylated by CYP2A6", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen · smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005789", "GO:0005788", "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76453", "summary": "Coumarin is 7-hydroxylated by CYP2A6 is a Reactome release 97 curated reaction event. The source record connects COUM, O2, NADPH to HCOU, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen · smooth endoplasmic reticulum.", "equation": "COUM + O2 + NADPH + H+ → HCOU + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-76328", "reactome_id": "R-ALL-76328", "label": "COUM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28794", "formula": "C9H6O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76452", "reactome_id": "R-ALL-76452", "label": "HCOU", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27510", "formula": "C9H6O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "coumarin 7-hydroxylase activity of Cytochrome P450 (CYP2A3 based) [endoplasmic reticulum membrane]", "activity_id": "76451", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15665333", "title": "Kinetic analysis of oxidation of coumarins by human cytochrome P450 2A6", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15665333/" } ], "x": 8018.666666666667, "y": 3035.6372549019607, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76456", "label": "O-atom dealkylation of dextromethorphan", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen · smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005789", "GO:0005788", "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76456", "summary": "O-atom dealkylation of dextromethorphan is a Reactome release 97 curated reaction event. The source record connects DEXM, NADPH, O2 to DEXT, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen · smooth endoplasmic reticulum.", "equation": "DEXM + NADPH + O2 + H+ → DEXT + H2O + NADP+ + CH2O", "inputs": [ { "node_id": "R-ALL-39703", "reactome_id": "R-ALL-39703", "label": "DEXM", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "ChemicalDrug", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76455", "reactome_id": "R-ALL-76455", "label": "DEXT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "ChemicalDrug", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-143501", "reactome_id": "R-ALL-143501", "label": "CH2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16842", "formula": "CH2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2D6 based) [endoplasmic reticulum membrane]", "activity_id": "76454", "ec_numbers": null, "accessions": [] } ], "citations": [], "x": 7740, "y": 3058.5196078431372, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76466", "label": "CYP4A11 omega-hydroxylates DDCX", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76466", "summary": "CYP4A11 omega-hydroxylates DDCX is a Reactome release 97 curated reaction event. The source record connects DDCX, O2, NADPH to 12OH-DDCX, H2O, NADP+ in smooth endoplasmic reticulum.", "equation": "DDCX + O2 + NADPH + H+ → 12OH-DDCX + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-33549", "reactome_id": "R-ALL-33549", "label": "DDCX", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18262", "formula": "C12H23O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-42373", "reactome_id": "R-ALL-42373", "label": "12OH-DDCX", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:39567", "formula": "C12H24O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP4A11 based) [endoplasmic reticulum membrane]", "activity_id": "76465", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8914854", "title": "Identification of CYP4A11 as the major lauric acid omega-hydroxylase in human liver microsomes", "journal": "Arch Biochem Biophys", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8914854/" } ], "x": 7461.333333333333, "y": 3043.264705882353, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76472", "label": "Ethylene is oxidized to Ethylene oxide by CYP1A1", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen · smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005789", "GO:0005788", "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76472", "summary": "Ethylene is oxidized to Ethylene oxide by CYP1A1 is a Reactome release 97 curated reaction event. The source record connects C2H4, O2, NADPH to EtO, H2O, NADP+ in endoplasmic reticulum membrane · endoplasmic reticulum lumen · smooth endoplasmic reticulum.", "equation": "C2H4 + O2 + NADPH + H+ → EtO + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-38985", "reactome_id": "R-ALL-38985", "label": "C2H4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18153", "formula": "C2H4", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-38987", "reactome_id": "R-ALL-38987", "label": "EtO", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27561", "formula": "C2H4O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A1 [endoplasmic reticulum membrane]", "activity_id": "76471", "ec_numbers": null, "accessions": [ "P04798" ] } ], "citations": [ { "id": "PMID:12464245", "title": "Cytochrome P450 oxidations in the generation of reactive electrophiles: epoxidation and related reactions", "journal": "Arch Biochem Biophys", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12464245/" }, { "id": "PMID:7217086", "title": "Destruction of cytochrome P-450 by ethylene. Structure of the resulting prosthetic heme adduct.", "journal": "J Biol Chem", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/7217086/" } ], "x": 7740, "y": 3043.264705882353, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76475", "label": "Dehalogenation of the poly-halogenated hydrocarbon Halothane to form the acylhalide Trifluoroacetlychloride and hydrogen bromide", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-76475", "summary": "Dehalogenation of the poly-halogenated hydrocarbon Halothane to form the acylhalide Trifluoroacetlychloride and hydrogen bromide is a Reactome release 97 curated reaction event. The source record connects HALO, NADPH, H+ to TFAC, HBr, NADP+ in smooth endoplasmic reticulum.", "equation": "HALO + NADPH + H+ + O2 → TFAC + HBr + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-40795", "reactome_id": "R-ALL-40795", "label": "HALO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:5615", "formula": "C2HBrClF3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76473", "reactome_id": "R-ALL-76473", "label": "TFAC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29138", "formula": "C2ClF3O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76474", "reactome_id": "R-ALL-76474", "label": "HBr", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:47266", "formula": "HBr", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Cytochrome P450 (CYP2E1 based) [endoplasmic reticulum membrane]", "activity_id": "76353", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9103523", "title": "Cytochrome P450 2E1 is the principal catalyst of human oxidative halothane metabolism in vitro", "journal": "J Pharmacol Exp Ther", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9103523/" }, { "id": "PMID:8637342", "title": "Identification of the enzyme responsible for oxidative halothane metabolism: implications for prevention of halothane hepatitis", "journal": "Lancet", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8637342/" } ], "x": 8018.666666666667, "y": 2944.107843137255, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76494", "label": "POR reduces CYP450:Fe3+ to CYP450:Fe2+", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:24041" ], "source_url": "https://reactome.org/content/detail/R-HSA-76494", "summary": "POR reduces CYP450:Fe3+ to CYP450:Fe2+ is a Reactome release 97 curated reaction event. The source record connects NADPH, CYP450:Fe3+ to NADP+, CYP450:Fe2+, H+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:24041.", "equation": "NADPH + 2 CYP450:Fe3+ → NADP+ + 2 CYP450:Fe2+ + H+", "inputs": [ { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-8957351", "reactome_id": "R-HSA-8957351", "label": "CYP450:Fe3+", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-8957354", "reactome_id": "R-HSA-8957354", "label": "CYP450:Fe2+", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NADPH-hemoprotein reductase activity of POR [endoplasmic reticulum membrane]", "activity_id": "76491", "ec_numbers": [ "1.6.2.4" ], "accessions": [ "P16435" ] } ], "citations": [ { "id": "PMID:12381719", "title": "Modification of the nucleotide cofactor-binding site of cytochrome P-450 reductase to enhance turnover with NADH in Vivo", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12381719/" }, { "id": "PMID:18838505", "title": "Role of NADPH-cytochrome P450 reductase and cytochrome-b5/NADH-b5 reductase in variability of CYP3A activity in human liver microsomes", "journal": "Drug Metab. Dispos.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18838505/" }, { "id": "PMID:31105987", "title": "Mechanisms of Cytochrome P450-Catalyzed Oxidations", "journal": "ACS Catal", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/31105987/" }, { "id": "PMID:4389465", "title": "Resolution of the cytochrome P-450-containing omega-hydroxylation system of liver microsomes into three components", "journal": "J Biol Chem", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/4389465/" }, { "id": "PMID:23353702", "title": "NADPH P450 oxidoreductase: structure, function, and pathology of diseases", "journal": "Pharmacol Ther", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23353702/" }, { "id": "PMID:1550342", "title": "Quantification of cytochrome P450 reductase gene expression in human tissues", "journal": "Arch. Biochem. Biophys.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550342/" } ], "x": 7740, "y": 2928.8529411764707, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76496", "label": "PTGIS, CYP8A1 isomerise PGH2 to PGI2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "biological-oxidations", "fatty-acid-metabolism", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:23581" ], "source_url": "https://reactome.org/content/detail/R-HSA-76496", "summary": "PTGIS, CYP8A1 isomerise PGH2 to PGI2 is a Reactome release 97 curated reaction event. The source record connects PGH2 to PGI2 in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:23581.", "equation": "PGH2 → PGI2", "inputs": [ { "node_id": "R-ALL-30138", "reactome_id": "R-ALL-30138", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-31593", "reactome_id": "R-ALL-31593", "label": "PGI2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57403", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "prostaglandin-I synthase activity of PTGIS,CYP8B1 [endoplasmic reticulum membrane]", "activity_id": "76495", "ec_numbers": [ "5.3.99.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:15115769", "title": "Purification and characterization of recombinant human prostacyclin synthase", "journal": "J Biochem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15115769/" }, { "id": "PMID:17641676", "title": "1-Methylnicotinamide (MNA), a primary metabolite of nicotinamide, exerts anti-thrombotic activity mediated by a cyclooxygenase-2/prostacyclin pathway", "journal": "Br. J. Pharmacol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17641676/" } ], "x": 590, "y": 1637.1052631578948, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76500", "label": "TBXAS1 isomerises PGH2 to TXA2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "biological-oxidations", "fatty-acid-metabolism", "phase-one" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:17138" ], "source_url": "https://reactome.org/content/detail/R-HSA-76500", "summary": "TBXAS1 isomerises PGH2 to TXA2 is a Reactome release 97 curated reaction event. The source record connects PGH2 to TXA2 in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:17138.", "equation": "PGH2 → TXA2", "inputs": [ { "node_id": "R-ALL-30138", "reactome_id": "R-ALL-30138", "label": "PGH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57405", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-32879", "reactome_id": "R-ALL-32879", "label": "TXA2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57445", "formula": "C20H31O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "thromboxane-A synthase activity of TBXAS1 [endoplasmic reticulum membrane]", "activity_id": "76499", "ec_numbers": [ "5.3.99.5" ], "accessions": [ "P24557" ] } ], "citations": [ { "id": "PMID:7925341", "title": "Characterization of the human gene (TBXAS1) encoding thromboxane synthase", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7925341/" }, { "id": "PMID:11465543", "title": "Identification of genetic variants in the human thromboxane synthase gene (CYP5A1)", "journal": "Mutat Res", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11465543/" } ], "x": 868.6666666666667, "y": 1637.1052631578948, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-76590", "label": "AMP + H2O =\u003e IMP + NH4+ (AMPD)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14778" ], "source_url": "https://reactome.org/content/detail/R-HSA-76590", "summary": "AMP + H2O =\u003e IMP + NH4+ (AMPD) is a Reactome release 97 curated reaction event. The source record connects AMP, H2O, H+ to NH4+, IMP in cytosol and cross-references RHEA:14778.", "equation": "AMP + H2O + H+ → NH4+ + IMP", "inputs": [ { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29602", "reactome_id": "R-ALL-29602", "label": "IMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58053", "formula": "C10H11N4O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "AMP deaminase activity of AMPD tetramers [cytosol]", "activity_id": "76587", "ec_numbers": [ "3.5.4.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:1429593", "title": "Molecular cloning of AMP deaminase isoform L. Sequence and bacterial expression of human AMPD2 cDNA", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1429593/" }, { "id": "PMID:9857047", "title": "Novel aspects of tetramer assembly and N-terminal domain structure and function are revealed by recombinant expression of human AMP deaminase isoforms", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9857047/" } ], "x": 3600, "y": 2856.3928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77254", "label": "(S)-3-Hydroxydodecanoyl-CoA+NAD =\u003e 3-Oxododecanoyl-CoA+NADH+H", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31180" ], "source_url": "https://reactome.org/content/detail/R-HSA-77254", "summary": "(S)-3-Hydroxydodecanoyl-CoA+NAD =\u003e 3-Oxododecanoyl-CoA+NADH+H is a Reactome release 97 curated reaction event. The source record connects NAD+, (S)-3-Hydroxydodecanoyl-CoA to NADH, H+, 3-Oxododecanoyl-CoA in mitochondrial matrix and cross-references RHEA:31180.", "equation": "NAD+ + (S)-3-Hydroxydodecanoyl-CoA → NADH + H+ + 3-Oxododecanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77252", "reactome_id": "R-ALL-77252", "label": "(S)-3-Hydroxydodecanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62558", "formula": "C33H54N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77253", "reactome_id": "R-ALL-77253", "label": "3-Oxododecanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62615", "formula": "C33H52N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of HADH dimer [mitochondrial matrix]", "activity_id": "71061", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:8687463", "title": "Human short-chain L-3-hydroxyacyl-CoA dehydrogenase: cloning and characterization of the coding sequence.", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8687463/" } ], "x": 1251.3333333333333, "y": 1967, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77256", "label": "2-trans-Dodecenoyl-CoA+H2O =\u003e( S)-3-Hydroxydodecanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31077" ], "source_url": "https://reactome.org/content/detail/R-HSA-77256", "summary": "2-trans-Dodecenoyl-CoA+H2O =\u003e( S)-3-Hydroxydodecanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects H2O, 2-trans-Dodecenoyl-CoA to (S)-3-Hydroxydodecanoyl-CoA in mitochondrial matrix and cross-references RHEA:31077.", "equation": "H2O + 2-trans-Dodecenoyl-CoA → (S)-3-Hydroxydodecanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77255", "reactome_id": "R-ALL-77255", "label": "2-trans-Dodecenoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57330", "formula": "C33H52N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-77252", "reactome_id": "R-ALL-77252", "label": "(S)-3-Hydroxydodecanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62558", "formula": "C33H54N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of ECHS1 hexamer [mitochondrial matrix]", "activity_id": "71049", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295248", "title": "Enzymes of fatty acid metabolism. II. Properties of crystalline crotonase.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295248/" } ], "x": 868.6666666666667, "y": 1612.9649122807018, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77263", "label": "lauroyl-CoA+FAD =\u003e 2-trans-Dodecenoyl-CoA+FADH2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31040" ], "source_url": "https://reactome.org/content/detail/R-HSA-77263", "summary": "lauroyl-CoA+FAD =\u003e 2-trans-Dodecenoyl-CoA+FADH2 is a Reactome release 97 curated reaction event. The source record connects FAD, LAU-CoA, H+ to FADH2, 2-trans-Dodecenoyl-CoA in mitochondrial matrix and cross-references RHEA:31040.", "equation": "FAD + LAU-CoA + H+ → FADH2 + 2-trans-Dodecenoyl-CoA", "inputs": [ { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77261", "reactome_id": "R-ALL-77261", "label": "LAU-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57375", "formula": "C33H54N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77255", "reactome_id": "R-ALL-77255", "label": "2-trans-Dodecenoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57330", "formula": "C33H52N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADL tetramer [mitochondrial matrix]", "activity_id": "77259", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295225", "title": "On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295225/" }, { "id": "PMID:1540149", "title": "Beta-oxidation of long-chain fatty acids by human fibroblasts: evidence for a novel long-chain acyl-coenzyme A dehydrogenase.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1540149/" } ], "x": 311.33333333333337, "y": 1561.6666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77271", "label": "3-Oxotetradecanoyl-CoA+CoA-SH =\u003e Lauroyl-CoA + acetyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31093" ], "source_url": "https://reactome.org/content/detail/R-HSA-77271", "summary": "3-Oxotetradecanoyl-CoA+CoA-SH =\u003e Lauroyl-CoA + acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-Oxotetradecanoyl-CoA, CoA-SH to LAU-CoA, Ac-CoA in mitochondrial matrix and cross-references RHEA:31093.", "equation": "3-Oxotetradecanoyl-CoA + CoA-SH → LAU-CoA + Ac-CoA", "inputs": [ { "node_id": "R-ALL-77269", "reactome_id": "R-ALL-77269", "label": "3-Oxotetradecanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62543", "formula": "C35H56N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77261", "reactome_id": "R-ALL-77261", "label": "LAU-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57375", "formula": "C33H54N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acyltransferase activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77268", "ec_numbers": [ "2.3.1.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 590, "y": 1588.8245614035088, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77274", "label": "myristoyl-CoA+FAD =\u003e trans-tetradec-2-enoyl-CoA+FADH2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:28359", "RHEA:47317" ], "source_url": "https://reactome.org/content/detail/R-HSA-77274", "summary": "myristoyl-CoA+FAD =\u003e trans-tetradec-2-enoyl-CoA+FADH2 is a Reactome release 97 curated reaction event. The source record connects FAD, MYS-CoA, H+ to FADH2, trans-Tetradec-2-enoyl-CoA in mitochondrial matrix and cross-references RHEA:28359, RHEA:47317.", "equation": "FAD + MYS-CoA + H+ → FADH2 + trans-Tetradec-2-enoyl-CoA", "inputs": [ { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77272", "reactome_id": "R-ALL-77272", "label": "MYS-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57385", "formula": "C35H58N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77273", "reactome_id": "R-ALL-77273", "label": "trans-Tetradec-2-enoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61405", "formula": "C35H56N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADL tetramer [mitochondrial matrix]", "activity_id": "77259", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295225", "title": "On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295225/" }, { "id": "PMID:1540149", "title": "Beta-oxidation of long-chain fatty acids by human fibroblasts: evidence for a novel long-chain acyl-coenzyme A dehydrogenase.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1540149/" } ], "x": 590, "y": 1561.6666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77277", "label": "trans-Tetradec-2-enoyl-CoA+H2O =\u003e (S)-3-Hydroxytetradecanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31173" ], "source_url": "https://reactome.org/content/detail/R-HSA-77277", "summary": "trans-Tetradec-2-enoyl-CoA+H2O =\u003e (S)-3-Hydroxytetradecanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects H2O, trans-Tetradec-2-enoyl-CoA to (S)-3-Hydroxytetradecanoyl-CoA in mitochondrial matrix and cross-references RHEA:31173.", "equation": "H2O + trans-Tetradec-2-enoyl-CoA → (S)-3-Hydroxytetradecanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77273", "reactome_id": "R-ALL-77273", "label": "trans-Tetradec-2-enoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61405", "formula": "C35H56N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-77276", "reactome_id": "R-ALL-77276", "label": "(S)-3-Hydroxytetradecanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62614", "formula": "C35H58N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77275", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 311.33333333333337, "y": 1615.982456140351, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77283", "label": "(S)-3-Hydroxytetradecanoyl-CoA+NAD =\u003e 3-Oxotetradecanoyl-CoA+NADH+H", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31168" ], "source_url": "https://reactome.org/content/detail/R-HSA-77283", "summary": "(S)-3-Hydroxytetradecanoyl-CoA+NAD =\u003e 3-Oxotetradecanoyl-CoA+NADH+H is a Reactome release 97 curated reaction event. The source record connects NAD+, (S)-3-Hydroxytetradecanoyl-CoA to NADH, H+, 3-Oxotetradecanoyl-CoA in mitochondrial matrix and cross-references RHEA:31168.", "equation": "NAD+ + (S)-3-Hydroxytetradecanoyl-CoA → NADH + H+ + 3-Oxotetradecanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77276", "reactome_id": "R-ALL-77276", "label": "(S)-3-Hydroxytetradecanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62614", "formula": "C35H58N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77269", "reactome_id": "R-ALL-77269", "label": "3-Oxotetradecanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62543", "formula": "C35H56N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77282", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 868.6666666666667, "y": 1561.6666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77299", "label": "palmitoyl-CoA+FAD =\u003e trans-Hexadec-2-enoyl-CoA+FADH2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:30748", "RHEA:43449" ], "source_url": "https://reactome.org/content/detail/R-HSA-77299", "summary": "palmitoyl-CoA+FAD =\u003e trans-Hexadec-2-enoyl-CoA+FADH2 is a Reactome release 97 curated reaction event. The source record connects PalmCoA, FAD, H+ to FADH2, trans-Hexadec-2-enoyl-CoA in mitochondrial matrix and cross-references RHEA:30748, RHEA:43449.", "equation": "PalmCoA + FAD + H+ → FADH2 + trans-Hexadec-2-enoyl-CoA", "inputs": [ { "node_id": "R-ALL-77281", "reactome_id": "R-ALL-77281", "label": "PalmCoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57379", "formula": "C37H62N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77284", "reactome_id": "R-ALL-77284", "label": "trans-Hexadec-2-enoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61526", "formula": "C37H60N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADVL dimer [mitochondrial matrix]", "activity_id": "77280", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295225", "title": "On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295225/" }, { "id": "PMID:1540149", "title": "Beta-oxidation of long-chain fatty acids by human fibroblasts: evidence for a novel long-chain acyl-coenzyme A dehydrogenase.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1540149/" } ], "x": 311.33333333333337, "y": 1564.6842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77301", "label": "trans-Hexadec-2-enoyl-CoA+H2O =\u003e (S)-3-Hydroxyhexadecanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31165" ], "source_url": "https://reactome.org/content/detail/R-HSA-77301", "summary": "trans-Hexadec-2-enoyl-CoA+H2O =\u003e (S)-3-Hydroxyhexadecanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects trans-Hexadec-2-enoyl-CoA, H2O to (S)-3-Hydroxyhexadecanoyl-CoA in mitochondrial matrix and cross-references RHEA:31165.", "equation": "trans-Hexadec-2-enoyl-CoA + H2O → (S)-3-Hydroxyhexadecanoyl-CoA", "inputs": [ { "node_id": "R-ALL-77284", "reactome_id": "R-ALL-77284", "label": "trans-Hexadec-2-enoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61526", "formula": "C37H60N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77300", "reactome_id": "R-ALL-77300", "label": "(S)-3-Hydroxyhexadecanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62613", "formula": "C37H62N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77275", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 590, "y": 1615.982456140351, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77303", "label": "(S)-3-Hydroxyhexadecanoyl-CoA+NAD =\u003e 3-Oxopalmitoyl-CoA+NADH+H", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31160" ], "source_url": "https://reactome.org/content/detail/R-HSA-77303", "summary": "(S)-3-Hydroxyhexadecanoyl-CoA+NAD =\u003e 3-Oxopalmitoyl-CoA+NADH+H is a Reactome release 97 curated reaction event. The source record connects (S)-3-Hydroxyhexadecanoyl-CoA, NAD+ to NADH, 3-Oxopalmitoyl-CoA, H+ in mitochondrial matrix and cross-references RHEA:31160.", "equation": "(S)-3-Hydroxyhexadecanoyl-CoA + NAD+ → NADH + 3-Oxopalmitoyl-CoA + H+", "inputs": [ { "node_id": "R-ALL-77300", "reactome_id": "R-ALL-77300", "label": "(S)-3-Hydroxyhexadecanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62613", "formula": "C37H62N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77302", "reactome_id": "R-ALL-77302", "label": "3-Oxopalmitoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57349", "formula": "C37H60N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77282", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 590, "y": 1564.6842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77304", "label": "3-Oxopalmitoyl-CoA+CoA-SH =\u003e myristoyl-CoA+acetyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18163" ], "source_url": "https://reactome.org/content/detail/R-HSA-77304", "summary": "3-Oxopalmitoyl-CoA+CoA-SH =\u003e myristoyl-CoA+acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-Oxopalmitoyl-CoA, CoA-SH to MYS-CoA, Ac-CoA in mitochondrial matrix and cross-references RHEA:18163.", "equation": "3-Oxopalmitoyl-CoA + CoA-SH → MYS-CoA + Ac-CoA", "inputs": [ { "node_id": "R-ALL-77302", "reactome_id": "R-ALL-77302", "label": "3-Oxopalmitoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57349", "formula": "C37H60N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77272", "reactome_id": "R-ALL-77272", "label": "MYS-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57385", "formula": "C35H58N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acyltransferase activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77268", "ec_numbers": [ "2.3.1.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 868.6666666666667, "y": 1588.8245614035088, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77309", "label": "3-Oxododecanoyl-CoA+CoA-SH =\u003e Decanoyl-CoA + acetyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31185" ], "source_url": "https://reactome.org/content/detail/R-HSA-77309", "summary": "3-Oxododecanoyl-CoA+CoA-SH =\u003e Decanoyl-CoA + acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-Oxododecanoyl-CoA, CoA-SH to DEC-CoA, Ac-CoA in mitochondrial matrix and cross-references RHEA:31185.", "equation": "3-Oxododecanoyl-CoA + CoA-SH → DEC-CoA + Ac-CoA", "inputs": [ { "node_id": "R-ALL-77253", "reactome_id": "R-ALL-77253", "label": "3-Oxododecanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62615", "formula": "C33H52N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77308", "reactome_id": "R-ALL-77308", "label": "DEC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61430", "formula": "C31H50N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acyltransferase activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77268", "ec_numbers": [ "2.3.1.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 311.33333333333337, "y": 1591.842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77312", "label": "(S)-Hydroxybutanoyl-CoA+NAD =\u003e Acetoacetyl-CoA+NADH+H", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:30800" ], "source_url": "https://reactome.org/content/detail/R-HSA-77312", "summary": "(S)-Hydroxybutanoyl-CoA+NAD =\u003e Acetoacetyl-CoA+NADH+H is a Reactome release 97 curated reaction event. The source record connects NAD+, 3HB-CoA to NADH, H+, ACA-CoA in mitochondrial matrix and cross-references RHEA:30800.", "equation": "NAD+ + 3HB-CoA → NADH + H+ + ACA-CoA", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77251", "reactome_id": "R-ALL-77251", "label": "3HB-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57316", "formula": "C25H38N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73871", "reactome_id": "R-ALL-73871", "label": "ACA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57286", "formula": "C25H36N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of HADH dimer [mitochondrial matrix]", "activity_id": "71061", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:8687463", "title": "Human short-chain L-3-hydroxyacyl-CoA dehydrogenase: cloning and characterization of the coding sequence.", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8687463/" }, { "id": "PMID:10231530", "title": "Biochemical characterization and crystal structure determination of human heart short chain L-3-hydroxyacyl-CoA dehydrogenase provide insights into catalytic mechanism.", "journal": "Biochemistry", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10231530/" } ], "x": 868.6666666666667, "y": 1564.6842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77314", "label": "Crotonoyl-CoA+H2O =\u003e (S)-3-Hydroxybutanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:26560" ], "source_url": "https://reactome.org/content/detail/R-HSA-77314", "summary": "Crotonoyl-CoA+H2O =\u003e (S)-3-Hydroxybutanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects H2O, Crotonoyl-CoA to 3HB-CoA in mitochondrial matrix and cross-references RHEA:26560.", "equation": "H2O + Crotonoyl-CoA → 3HB-CoA", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77313", "reactome_id": "R-ALL-77313", "label": "Crotonoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57332", "formula": "C25H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-77251", "reactome_id": "R-ALL-77251", "label": "3HB-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57316", "formula": "C25H38N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of ECHS1 hexamer [mitochondrial matrix]", "activity_id": "71049", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295248", "title": "Enzymes of fatty acid metabolism. II. Properties of crystalline crotonase.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295248/" } ], "x": 868.6666666666667, "y": 1615.982456140351, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77319", "label": "Butanoyl-CoA+FAD =\u003e Crotonoyl-CoA+FADH2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:30732" ], "source_url": "https://reactome.org/content/detail/R-HSA-77319", "summary": "Butanoyl-CoA+FAD =\u003e Crotonoyl-CoA+FADH2 is a Reactome release 97 curated reaction event. The source record connects FAD, BT-CoA, H+ to FADH2, Crotonoyl-CoA in mitochondrial matrix and cross-references RHEA:30732.", "equation": "FAD + BT-CoA + H+ → FADH2 + Crotonoyl-CoA", "inputs": [ { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77318", "reactome_id": "R-ALL-77318", "label": "BT-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57371", "formula": "C25H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77313", "reactome_id": "R-ALL-77313", "label": "Crotonoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57332", "formula": "C25H36N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADS tetramer [mitochondrial matrix]", "activity_id": "77317", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295225", "title": "On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295225/" }, { "id": "PMID:3597357", "title": "Purification and properties of short chain acyl-CoA, medium chain acyl-CoA, and isovaleryl-CoA dehydrogenases from human liver.", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3597357/" } ], "x": 311.33333333333337, "y": 1567.701754385965, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77321", "label": "3-Oxohexanoyl-CoA+CoA-SH =\u003e Butanoyl-CoA + acetyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31113" ], "source_url": "https://reactome.org/content/detail/R-HSA-77321", "summary": "3-Oxohexanoyl-CoA+CoA-SH =\u003e Butanoyl-CoA + acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-Oxohexanoyl-CoA, CoA-SH to Ac-CoA, BT-CoA in mitochondrial matrix and cross-references RHEA:31113.", "equation": "3-Oxohexanoyl-CoA + CoA-SH → Ac-CoA + BT-CoA", "inputs": [ { "node_id": "R-ALL-77320", "reactome_id": "R-ALL-77320", "label": "3-Oxohexanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62418", "formula": "C27H40N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77318", "reactome_id": "R-ALL-77318", "label": "BT-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57371", "formula": "C25H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acyltransferase activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77268", "ec_numbers": [ "2.3.1.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 590, "y": 1591.842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77323", "label": "(S)-Hydroxyhexanoyl-CoA+NAD =\u003e 3-Oxohexanoyl-CoA+NADH+H", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31144" ], "source_url": "https://reactome.org/content/detail/R-HSA-77323", "summary": "(S)-Hydroxyhexanoyl-CoA+NAD =\u003e 3-Oxohexanoyl-CoA+NADH+H is a Reactome release 97 curated reaction event. The source record connects NAD+, (S)-Hydroxyhexanoyl-CoA to NADH, H+, 3-Oxohexanoyl-CoA in mitochondrial matrix and cross-references RHEA:31144.", "equation": "NAD+ + (S)-Hydroxyhexanoyl-CoA → NADH + H+ + 3-Oxohexanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77322", "reactome_id": "R-ALL-77322", "label": "(S)-Hydroxyhexanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62075", "formula": "C27H42N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77320", "reactome_id": "R-ALL-77320", "label": "3-Oxohexanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62418", "formula": "C27H40N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of HADH dimer [mitochondrial matrix]", "activity_id": "71061", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:8687463", "title": "Human short-chain L-3-hydroxyacyl-CoA dehydrogenase: cloning and characterization of the coding sequence.", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8687463/" } ], "x": 590, "y": 1567.701754385965, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77325", "label": "trans-Hex-2-enoyl-CoA+H2O (S)-Hydroxyhexanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:30549" ], "source_url": "https://reactome.org/content/detail/R-HSA-77325", "summary": "trans-Hex-2-enoyl-CoA+H2O (S)-Hydroxyhexanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects H2O, trans-Hex-2-enoyl-CoA to (S)-Hydroxyhexanoyl-CoA in mitochondrial matrix and cross-references RHEA:30549.", "equation": "H2O + trans-Hex-2-enoyl-CoA → (S)-Hydroxyhexanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77324", "reactome_id": "R-ALL-77324", "label": "trans-Hex-2-enoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62077", "formula": "C27H40N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-77322", "reactome_id": "R-ALL-77322", "label": "(S)-Hydroxyhexanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62075", "formula": "C27H42N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of ECHS1 hexamer [mitochondrial matrix]", "activity_id": "71049", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295248", "title": "Enzymes of fatty acid metabolism. II. Properties of crystalline crotonase.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295248/" } ], "x": 311.33333333333337, "y": 1619, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77327", "label": "Hexanoyl-CoA+FAD =\u003e trans-Hex-2-enoyl-CoA+FADH2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31208" ], "source_url": "https://reactome.org/content/detail/R-HSA-77327", "summary": "Hexanoyl-CoA+FAD =\u003e trans-Hex-2-enoyl-CoA+FADH2 is a Reactome release 97 curated reaction event. The source record connects FAD, HX-CoA, H+ to FADH2, trans-Hex-2-enoyl-CoA in mitochondrial matrix and cross-references RHEA:31208.", "equation": "FAD + HX-CoA + H+ → FADH2 + trans-Hex-2-enoyl-CoA", "inputs": [ { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77326", "reactome_id": "R-ALL-77326", "label": "HX-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62620", "formula": "C27H42N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77324", "reactome_id": "R-ALL-77324", "label": "trans-Hex-2-enoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62077", "formula": "C27H40N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADS tetramer [mitochondrial matrix]", "activity_id": "77317", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295225", "title": "On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295225/" }, { "id": "PMID:3597357", "title": "Purification and properties of short chain acyl-CoA, medium chain acyl-CoA, and isovaleryl-CoA dehydrogenases from human liver.", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3597357/" } ], "x": 868.6666666666667, "y": 1567.701754385965, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77329", "label": "3-Oxooctanoyl-CoA+CoA-SH =\u003e Hexanoyl-CoA + acetyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31205" ], "source_url": "https://reactome.org/content/detail/R-HSA-77329", "summary": "3-Oxooctanoyl-CoA+CoA-SH =\u003e Hexanoyl-CoA + acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-Oxooctanoyl-CoA, CoA-SH to HX-CoA, Ac-CoA in mitochondrial matrix and cross-references RHEA:31205.", "equation": "3-Oxooctanoyl-CoA + CoA-SH → HX-CoA + Ac-CoA", "inputs": [ { "node_id": "R-ALL-77328", "reactome_id": "R-ALL-77328", "label": "3-Oxooctanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62619", "formula": "C29H44N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77326", "reactome_id": "R-ALL-77326", "label": "HX-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62620", "formula": "C27H42N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acyltransferase activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77268", "ec_numbers": [ "2.3.1.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 868.6666666666667, "y": 1591.842105263158, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77331", "label": "(S)-Hydroxyoctanoyl-CoA+NAD =\u003e 3-Oxooctanoyl-CoA+NADH+H", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31196" ], "source_url": "https://reactome.org/content/detail/R-HSA-77331", "summary": "(S)-Hydroxyoctanoyl-CoA+NAD =\u003e 3-Oxooctanoyl-CoA+NADH+H is a Reactome release 97 curated reaction event. The source record connects NAD+, (S)-Hydroxyoctanoyl-CoA to NADH, H+, 3-Oxooctanoyl-CoA in mitochondrial matrix and cross-references RHEA:31196.", "equation": "NAD+ + (S)-Hydroxyoctanoyl-CoA → NADH + H+ + 3-Oxooctanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77330", "reactome_id": "R-ALL-77330", "label": "(S)-Hydroxyoctanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62617", "formula": "C29H46N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77328", "reactome_id": "R-ALL-77328", "label": "3-Oxooctanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62619", "formula": "C29H44N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of HADH dimer [mitochondrial matrix]", "activity_id": "71061", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:8687463", "title": "Human short-chain L-3-hydroxyacyl-CoA dehydrogenase: cloning and characterization of the coding sequence.", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8687463/" } ], "x": 311.33333333333337, "y": 1570.719298245614, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77333", "label": "trans-Oct-2-enoyl-CoA+H2O =\u003e (S)-Hydroxyoctanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31201" ], "source_url": "https://reactome.org/content/detail/R-HSA-77333", "summary": "trans-Oct-2-enoyl-CoA+H2O =\u003e (S)-Hydroxyoctanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects H2O, trans-Oct-2-enoyl-CoA to (S)-Hydroxyoctanoyl-CoA in mitochondrial matrix and cross-references RHEA:31201.", "equation": "H2O + trans-Oct-2-enoyl-CoA → (S)-Hydroxyoctanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77332", "reactome_id": "R-ALL-77332", "label": "trans-Oct-2-enoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62242", "formula": "C29H44N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-77330", "reactome_id": "R-ALL-77330", "label": "(S)-Hydroxyoctanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62617", "formula": "C29H46N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of ECHS1 hexamer [mitochondrial matrix]", "activity_id": "71049", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295248", "title": "Enzymes of fatty acid metabolism. II. Properties of crystalline crotonase.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295248/" } ], "x": 590, "y": 1619, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77338", "label": "Octanoyl-CoA+FAD =\u003e trans-Oct-2-enoyl-CoA+FADH2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:30944" ], "source_url": "https://reactome.org/content/detail/R-HSA-77338", "summary": "Octanoyl-CoA+FAD =\u003e trans-Oct-2-enoyl-CoA+FADH2 is a Reactome release 97 curated reaction event. The source record connects FAD, Octanoyl-CoA, H+ to FADH2, trans-Oct-2-enoyl-CoA in mitochondrial matrix and cross-references RHEA:30944.", "equation": "FAD + Octanoyl-CoA + H+ → FADH2 + trans-Oct-2-enoyl-CoA", "inputs": [ { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77337", "reactome_id": "R-ALL-77337", "label": "Octanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57386", "formula": "C29H46N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77332", "reactome_id": "R-ALL-77332", "label": "trans-Oct-2-enoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62242", "formula": "C29H44N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADM tetramer [mitochondrial matrix]", "activity_id": "77336", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295225", "title": "On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295225/" }, { "id": "PMID:3597357", "title": "Purification and properties of short chain acyl-CoA, medium chain acyl-CoA, and isovaleryl-CoA dehydrogenases from human liver.", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3597357/" } ], "x": 590, "y": 1570.719298245614, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77340", "label": "3-Oxodecanoyl-CoA+CoA-SH =\u003e Octanoyl-CoA + acetyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31089" ], "source_url": "https://reactome.org/content/detail/R-HSA-77340", "summary": "3-Oxodecanoyl-CoA+CoA-SH =\u003e Octanoyl-CoA + acetyl-CoA is a Reactome release 97 curated reaction event. The source record connects 3-Oxodecanoyl-CoA, CoA-SH to Octanoyl-CoA, Ac-CoA in mitochondrial matrix and cross-references RHEA:31089.", "equation": "3-Oxodecanoyl-CoA + CoA-SH → Octanoyl-CoA + Ac-CoA", "inputs": [ { "node_id": "R-ALL-77339", "reactome_id": "R-ALL-77339", "label": "3-Oxodecanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62548", "formula": "C31H48N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77337", "reactome_id": "R-ALL-77337", "label": "Octanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57386", "formula": "C29H46N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA C-acyltransferase activity of Trifunctional Protein [mitochondrial matrix]", "activity_id": "77268", "ec_numbers": [ "2.3.1.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:1550553", "title": "Human liver long-chain 3-hydroxyacyl-coenzyme A dehydrogenase is a multifunctional membrane-bound beta-oxidation enzyme of mitochondria.", "journal": "Biochem Biophys Res Commun", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1550553/" } ], "x": 311.33333333333337, "y": 1594.859649122807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77342", "label": "(S)-Hydroxydecanoyl-CoA+NAD =\u003e 3-Oxodecanoyl-CoA+NADH+H", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31188" ], "source_url": "https://reactome.org/content/detail/R-HSA-77342", "summary": "(S)-Hydroxydecanoyl-CoA+NAD =\u003e 3-Oxodecanoyl-CoA+NADH+H is a Reactome release 97 curated reaction event. The source record connects NAD+, (S)-Hydroxydecanoyl-CoA to NADH, H+, 3-Oxodecanoyl-CoA in mitochondrial matrix and cross-references RHEA:31188.", "equation": "NAD+ + (S)-Hydroxydecanoyl-CoA → NADH + H+ + 3-Oxodecanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77341", "reactome_id": "R-ALL-77341", "label": "(S)-Hydroxydecanoyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62616", "formula": "C31H50N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77339", "reactome_id": "R-ALL-77339", "label": "3-Oxodecanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62548", "formula": "C31H48N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity of HADH dimer [mitochondrial matrix]", "activity_id": "71061", "ec_numbers": [ "1.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:8687463", "title": "Human short-chain L-3-hydroxyacyl-CoA dehydrogenase: cloning and characterization of the coding sequence.", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8687463/" } ], "x": 868.6666666666667, "y": 1570.719298245614, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77344", "label": "trans-Dec-2-enoyl-CoA+H2O =\u003e (S)-Hydroxydecanoyl-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31193" ], "source_url": "https://reactome.org/content/detail/R-HSA-77344", "summary": "trans-Dec-2-enoyl-CoA+H2O =\u003e (S)-Hydroxydecanoyl-CoA is a Reactome release 97 curated reaction event. The source record connects H2O, tdec2-CoA to (S)-Hydroxydecanoyl-CoA in mitochondrial matrix and cross-references RHEA:31193.", "equation": "H2O + tdec2-CoA → (S)-Hydroxydecanoyl-CoA", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-77343", "reactome_id": "R-ALL-77343", "label": "tdec2-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61406", "formula": "C31H48N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-77341", "reactome_id": "R-ALL-77341", "label": "(S)-Hydroxydecanoyl-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62616", "formula": "C31H50N7O18P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "enoyl-CoA hydratase activity of ECHS1 hexamer [mitochondrial matrix]", "activity_id": "71049", "ec_numbers": [ "4.2.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295248", "title": "Enzymes of fatty acid metabolism. II. Properties of crystalline crotonase.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295248/" } ], "x": 868.6666666666667, "y": 1619, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-77345", "label": "Decanoyl-CoA+FAD =\u003e trans-Dec-2-enoyl-CoA+FADH2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:28355" ], "source_url": "https://reactome.org/content/detail/R-HSA-77345", "summary": "Decanoyl-CoA+FAD =\u003e trans-Dec-2-enoyl-CoA+FADH2 is a Reactome release 97 curated reaction event. The source record connects FAD, DEC-CoA, H+ to FADH2, tdec2-CoA in mitochondrial matrix and cross-references RHEA:28355.", "equation": "FAD + DEC-CoA + H+ → FADH2 + tdec2-CoA", "inputs": [ { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77308", "reactome_id": "R-ALL-77308", "label": "DEC-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61430", "formula": "C31H50N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-31649", "reactome_id": "R-ALL-31649", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-77343", "reactome_id": "R-ALL-77343", "label": "tdec2-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61406", "formula": "C31H48N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-CoA dehydrogenase activity of ACADM tetramer [mitochondrial matrix]", "activity_id": "77336", "ec_numbers": [ "1.3.99.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:13295225", "title": "On the mechanism of dehydrogenation of fatty acyl derivatives of coenzyme A. II. The electron-transferring flavoprotein.", "journal": "J Biol Chem", "year": 1956, "url": "https://pubmed.ncbi.nlm.nih.gov/13295225/" }, { "id": "PMID:3597357", "title": "Purification and properties of short chain acyl-CoA, medium chain acyl-CoA, and isovaleryl-CoA dehydrogenases from human liver.", "journal": "J Biol Chem", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/3597357/" } ], "x": 311.33333333333337, "y": 1573.7368421052631, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-804969", "label": "HSD17B1 hydrogenates E1 to EST17b", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24618" ], "source_url": "https://reactome.org/content/detail/R-HSA-804969", "summary": "HSD17B1 hydrogenates E1 to EST17b is a Reactome release 97 curated reaction event. The source record connects E1, H+, NADPH to EST17b, NADP+ in cytosol and cross-references RHEA:24618.", "equation": "E1 + H+ + NADPH → EST17b + NADP+", "inputs": [ { "node_id": "R-ALL-193066", "reactome_id": "R-ALL-193066", "label": "E1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17263", "formula": "C18H22O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-193132", "reactome_id": "R-ALL-193132", "label": "EST17b", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16469", "formula": "C18H24O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "estradiol 17-beta-dehydrogenase [NAD(P)+] activity of HSD17B1 dimer [cytosol]", "activity_id": "804965", "ec_numbers": [ "1.1.1.62" ], "accessions": [] } ], "citations": [ { "id": "PMID:19027824", "title": "Integrated view on 17beta-hydroxysteroid dehydrogenases", "journal": "Mol Cell Endocrinol.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19027824/" } ], "x": 4451.333333333333, "y": 1618.4621212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8847579", "label": "SCD5 desaturates ST-CoA to OLE-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:19722" ], "source_url": "https://reactome.org/content/detail/R-HSA-8847579", "summary": "SCD5 desaturates ST-CoA to OLE-CoA is a Reactome release 97 curated reaction event. The source record connects ST-CoA, H+, O2 to OLE-CoA, Fe(3+)Cb5, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:19722.", "equation": "ST-CoA + 2 H+ + O2 + 2 Fe(2+)Cb5 → OLE-CoA + 2 Fe(3+)Cb5 + 2 H2O", "inputs": [ { "node_id": "R-ALL-428139", "reactome_id": "R-ALL-428139", "label": "ST-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57394", "formula": "C39H66N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-4085415", "reactome_id": "R-ALL-4085415", "label": "Fe(2+)Cb5", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:16518", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5690558", "reactome_id": "R-ALL-5690558", "label": "OLE-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57387", "formula": "C39H64N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-4085425", "reactome_id": "R-ALL-4085425", "label": "Fe(3+)Cb5", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18097", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "stearoyl-CoA 9-desaturase activity of SCD5 dimer [endoplasmic reticulum membrane]", "activity_id": "8847576", "ec_numbers": [ "1.14.19.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:15907797", "title": "Characterization of HSCD5, a novel human stearoyl-CoA desaturase unique to primates", "journal": "Biochem. Biophys. Res. Commun.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15907797/" }, { "id": "PMID:15610069", "title": "Characterization of human SCD2, an oligomeric desaturase with improved stability and enzyme activity by cross-linking in intact cells", "journal": "Biochem. J.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15610069/" } ], "x": 311.33333333333337, "y": 1658.2280701754385, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8847912", "label": "PNPLA7 hydrolyzes LysoPtdCho", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8847912", "summary": "PNPLA7 hydrolyzes LysoPtdCho is a Reactome release 97 curated reaction event. The source record connects LysoPtdCho, H2O to GPCho, LCFA(-) in endoplasmic reticulum membrane · cytosol.", "equation": "LysoPtdCho + H2O → GPCho + LCFA(-)", "inputs": [ { "node_id": "R-ALL-6814253", "reactome_id": "R-ALL-6814253", "label": "LysoPtdCho", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58168", "formula": "C9H19NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1498750", "reactome_id": "R-ALL-1498750", "label": "GPCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16870", "formula": "C8H20NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524021", "reactome_id": "R-ALL-1524021", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine lysophospholipase A1 activity of PNPLA7 [endoplasmic reticulum membrane]", "activity_id": "8847901", "ec_numbers": [ "3.1.1.5" ], "accessions": [ "Q6ZV29" ] } ], "citations": [ { "id": "PMID:18086666", "title": "Identification of an insulin-regulated lysophospholipase with homology to neuropathy target esterase", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18086666/" }, { "id": "PMID:16799181", "title": "Characterization of the human patatin-like phospholipase family", "journal": "J. Lipid Res.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16799181/" } ], "x": 2381.3333333333335, "y": 1834.0753424657535, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8848215", "label": "ACAT2 condenses 2 Ac-CoA to form ACA-CoA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21037" ], "source_url": "https://reactome.org/content/detail/R-HSA-8848215", "summary": "ACAT2 condenses 2 Ac-CoA to form ACA-CoA is a Reactome release 97 curated reaction event. The source record connects Ac-CoA to ACA-CoA, CoA-SH, H2O in cytosol and cross-references RHEA:21037.", "equation": "2 Ac-CoA → ACA-CoA + CoA-SH + H2O", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-191293", "reactome_id": "R-ALL-191293", "label": "ACA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57286", "formula": "C25H36N7O18P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acetyl-CoA C-acetyltransferase activity of ACAT2 tetramer [cytosol]", "activity_id": "8848220", "ec_numbers": [ "2.3.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:7911016", "title": "Molecular cloning and nucleotide sequence of complementary DNA for human hepatic cytosolic acetoacetyl-coenzyme A thiolase", "journal": "Biochem. Biophys. Res. Commun.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7911016/" }, { "id": "PMID:9380443", "title": "Enzymes of ketone body utilization in human tissues: protein and messenger RNA levels of succinyl-coenzyme A (CoA):3-ketoacid CoA transferase and mitochondrial and cytosolic acetoacetyl-CoA thiolases", "journal": "Pediatr. Res.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9380443/" } ], "x": 2938.666666666667, "y": 2206.5, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8848246", "label": "DBI, ACBD7 bind MCFA-CoA and LCFA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8848246", "summary": "DBI, ACBD7 bind MCFA-CoA and LCFA-CoA is a Reactome release 97 curated reaction event. The source record connects DBI, (ACBD7), MCFA-CoA, LCFA-CoA to DBI,ACBD7:MCFA-CoA,LCFA-CoA in endoplasmic reticulum lumen.", "equation": "DBI, (ACBD7) + MCFA-CoA, LCFA-CoA → DBI,ACBD7:MCFA-CoA,LCFA-CoA", "inputs": [ { "node_id": "R-HSA-8848239", "reactome_id": "R-HSA-8848239", "label": "DBI, (ACBD7)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "CandidateSet", "currency": false }, { "node_id": "R-ALL-8848288", "reactome_id": "R-ALL-8848288", "label": "MCFA-CoA, LCFA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8848291", "reactome_id": "R-HSA-8848291", "label": "DBI,ACBD7:MCFA-CoA,LCFA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:17953517", "title": "Acyl-CoA-binding protein (ACBP) localizes to the endoplasmic reticulum and Golgi in a ligand-dependent manner in mammalian cells", "journal": "Biochem. J.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17953517/" }, { "id": "PMID:24080521", "title": "Acyl-CoA binding protein and epidermal barrier function", "journal": "Biochim. Biophys. Acta", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24080521/" } ], "x": 590, "y": 1748.7543859649122, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8848247", "label": "ACBD4,5 bind MCFA-CoA and LCFA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal membrane · peroxisomal matrix", "compartment_ids": [ "GO:0005778", "GO:0005782" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8848247", "summary": "ACBD4,5 bind MCFA-CoA and LCFA-CoA is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ACBD5,(ACBD4), MCFA-CoA, LCFA-CoA to ACBD4,5:LCFA-CoA, MCFA-CoA in peroxisomal membrane · peroxisomal matrix.", "equation": "ACBD5,(ACBD4) + MCFA-CoA, LCFA-CoA → ACBD4,5:LCFA-CoA, MCFA-CoA", "inputs": [ { "node_id": "R-HSA-8848234", "reactome_id": "R-HSA-8848234", "label": "ACBD5,(ACBD4)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal membrane", "entity_kind": "CandidateSet", "currency": false }, { "node_id": "R-ALL-5690520", "reactome_id": "R-ALL-5690520", "label": "MCFA-CoA, LCFA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal matrix", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8866017", "reactome_id": "R-HSA-8866017", "label": "ACBD4,5:LCFA-CoA, MCFA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "peroxisomal membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "fatty-acyl-CoA binding activity of ACBD5,(ACBD4) [peroxisomal membrane]", "activity_id": "9917327", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:24535825", "title": "Peroxisomal Atg37 binds Atg30 or palmitoyl-CoA to regulate phagophore formation during pexophagy", "journal": "J. Cell Biol.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24535825/" }, { "id": "PMID:24905344", "title": "Atg37 regulates the assembly of the pexophagic receptor protein complex", "journal": "Autophagy", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24905344/" } ], "x": 311.33333333333337, "y": 1742.719298245614, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8848250", "label": "ACBD6 binds LCFA-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8848250", "summary": "ACBD6 binds LCFA-CoA is a Reactome release 97 curated reaction event. The source record connects ACBD6, LCFA-CoA to ACBD6:LCFA-CoA in cytosol.", "equation": "ACBD6 + LCFA-CoA → ACBD6:LCFA-CoA", "inputs": [ { "node_id": "R-HSA-8848243", "reactome_id": "R-HSA-8848243", "label": "ACBD6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-164991", "reactome_id": "R-ALL-164991", "label": "LCFA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83139", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8848271", "reactome_id": "R-HSA-8848271", "label": "ACBD6:LCFA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:18268358", "title": "Characterization of an acyl-coenzyme A binding protein predominantly expressed in human primitive progenitor cells", "journal": "J. Lipid Res.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18268358/" }, { "id": "PMID:26290611", "title": "Ligand binding to the ACBD6 protein regulates the acyl-CoA transferase reactions in membranes", "journal": "J. Lipid Res.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26290611/" } ], "x": 868.6666666666667, "y": 1745.7368421052631, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8848338", "label": "PNPLA4 hydrolyzes TAG", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:12045" ], "source_url": "https://reactome.org/content/detail/R-HSA-8848338", "summary": "PNPLA4 hydrolyzes TAG is a Reactome release 97 curated reaction event. The source record connects TAG, H2O to DAG, LCFA(-), H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:12045.", "equation": "TAG + H2O → DAG + LCFA(-) + H+", "inputs": [ { "node_id": "R-ALL-549167", "reactome_id": "R-ALL-549167", "label": "TAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76103", "reactome_id": "R-ALL-76103", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8876730", "reactome_id": "R-ALL-8876730", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "triacylglycerol lipase activity of PNPLA4 [cytosol]", "activity_id": "8848353", "ec_numbers": [ "3.1.1.3" ], "accessions": [ "P41247" ] } ], "citations": [ { "id": "PMID:17603008", "title": "A comparative study of human GS2, its paralogues, and its rat orthologue", "journal": "Biochem. Biophys. Res. Commun.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17603008/" }, { "id": "PMID:15364929", "title": "Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15364929/" } ], "x": 3600, "y": 2036.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8848339", "label": "PNPLA5 hydrolyzes TAG", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:12045" ], "source_url": "https://reactome.org/content/detail/R-HSA-8848339", "summary": "PNPLA5 hydrolyzes TAG is a Reactome release 97 curated reaction event. The source record connects TAG, H2O to DAG, LCFA(-), H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:12045.", "equation": "TAG + H2O → DAG + LCFA(-) + H+", "inputs": [ { "node_id": "R-ALL-549167", "reactome_id": "R-ALL-549167", "label": "TAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-76103", "reactome_id": "R-ALL-76103", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8876730", "reactome_id": "R-ALL-8876730", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "triacylglycerol lipase activity of PNPLA5 [cytosol]", "activity_id": "8848358", "ec_numbers": [ "3.1.1.3" ], "accessions": [ "Q7Z6Z6" ] } ], "citations": [ { "id": "PMID:17603008", "title": "A comparative study of human GS2, its paralogues, and its rat orthologue", "journal": "Biochem. Biophys. Res. Commun.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17603008/" } ], "x": 3878.666666666667, "y": 2036.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8848355", "label": "PNPLA4 hydrolyzes retinyl palmitate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "triglycerides" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13934" ], "source_url": "https://reactome.org/content/detail/R-HSA-8848355", "summary": "PNPLA4 hydrolyzes retinyl palmitate is a Reactome release 97 curated reaction event. The source record connects atR-PALM, H2O to atROL, PALM(-), H+ in cytosol and cross-references RHEA:13934.", "equation": "atR-PALM + H2O → atROL + PALM(-) + H+", "inputs": [ { "node_id": "R-ALL-2534407", "reactome_id": "R-ALL-2534407", "label": "atR-PALM", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17616", "formula": "C36H60O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-30209", "reactome_id": "R-ALL-30209", "label": "atROL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17336", "formula": "C20H30O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8848337", "reactome_id": "R-ALL-8848337", "label": "PALM(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:7896", "formula": "C16H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "retinyl-palmitate esterase activity of PNPLA4 [cytosol]", "activity_id": "8848341", "ec_numbers": [ "3.1.1.21" ], "accessions": [ "P41247" ] } ], "citations": [ { "id": "PMID:16741517", "title": "Molecular screening for GS2 lipase regulators: inhibition of keratinocyte retinylester hydrolysis by TIP47", "journal": "J. Invest. Dermatol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16741517/" }, { "id": "PMID:17603008", "title": "A comparative study of human GS2, its paralogues, and its rat orthologue", "journal": "Biochem. Biophys. Res. Commun.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17603008/" }, { "id": "PMID:19181555", "title": "GS2 as a retinol transacylase and as a catalytic dyad independent regulator of retinylester accretion", "journal": "Mol. Genet. Metab.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19181555/" } ], "x": 3321.3333333333335, "y": 2036.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8848580", "label": "DGAT2L6,L7P transfer acyl group from acyl-CoA to DAG, forming TAG", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8848580", "summary": "DGAT2L6,L7P transfer acyl group from acyl-CoA to DAG, forming TAG is a Reactome release 97 curated reaction event. The source record connects DAG, acyl-CoA to TAG, CoA-SH in endoplasmic reticulum membrane · cytosol.", "equation": "DAG + acyl-CoA → TAG + CoA-SH", "inputs": [ { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500578", "reactome_id": "R-ALL-1500578", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1500594", "reactome_id": "R-ALL-1500594", "label": "TAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17855", "formula": "C6H5O6R3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "diacylglycerol O-acyltransferase activity of DGAT2L6 [endoplasmic reticulum membrane]", "activity_id": "8848579", "ec_numbers": [ "2.3.1.20" ], "accessions": [ "Q6ZPD8" ] } ], "citations": [ { "id": "PMID:15671038", "title": "Identification of two novel human acyl-CoA wax alcohol acyltransferases: members of the diacylglycerol acyltransferase 2 (DGAT2) gene superfamily", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15671038/" } ], "x": 2660, "y": 1834.0753424657535, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8849969", "label": "PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3 are dephosphorylated to PI5P, PI3P and PI(3,4)P by INPP5F at the endosome membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · early endosome membrane", "compartment_ids": [ "GO:0005829", "GO:0031901" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8849969", "summary": "PI(4,5)P2, PI(3,4)P2 and PI(3,4,5)P3 are dephosphorylated to PI5P, PI3P and PI(3,4)P by INPP5F at the endosome membrane is a Reactome release 97 curated reaction event. The source record connects PI(4,5)P2, PI(3,4)P2, PI(3,4,5)P3, H2O to PI5P, PI3P, PI(3,5)P2, Pi in cytosol · early endosome membrane.", "equation": "PI(4,5)P2, PI(3,4)P2, PI(3,4,5)P3 + H2O → PI5P, PI3P, PI(3,5)P2 + Pi", "inputs": [ { "node_id": "R-ALL-8849959", "reactome_id": "R-ALL-8849959", "label": "PI(4,5)P2, PI(3,4)P2, PI(3,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8849958", "reactome_id": "R-ALL-8849958", "label": "PI5P, PI3P, PI(3,5)P2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol phosphate 4-phosphatase activity of INPP5F [early endosome membrane]", "activity_id": "8849954", "ec_numbers": null, "accessions": [ "Q9Y2H2" ] } ], "citations": [ { "id": "PMID:25869668", "title": "Sac2/INPP5F is an inositol 4-phosphatase that functions in the endocytic pathway", "journal": "J. Cell Biol.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25869668/" } ], "x": 2938.666666666667, "y": 1855.390410958904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8850846", "label": "NTPDase1 hydrolyzes nucleoside triphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:23681" ], "source_url": "https://reactome.org/content/detail/R-HSA-8850846", "summary": "NTPDase1 hydrolyzes nucleoside triphosphates is a Reactome release 97 curated reaction event. The source record connects NTP, H2O to NDP, Pi, H+ in extracellular region · plasma membrane and cross-references RHEA:23681.", "equation": "NTP + H2O → NDP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8870907", "reactome_id": "R-ALL-8870907", "label": "NTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61557", "formula": "C5H8O13P3R", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8870906", "reactome_id": "R-ALL-8870906", "label": "NDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside triphosphate phosphatase activity of NTPDase1:Ca2+,Mg2+ [plasma membrane]", "activity_id": "8850850", "ec_numbers": [ "3.6.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:8955160", "title": "Identification and characterization of CD39/vascular ATP diphosphohydrolase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8955160/" }, { "id": "PMID:11927941", "title": "CD39 is the dominant Langerhans cell-associated ecto-NTPDase: modulatory roles in inflammation and immune responsiveness", "journal": "Nat. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11927941/" }, { "id": "PMID:10866813", "title": "Distribution, cloning, and characterization of porcine nucleoside triphosphate diphosphohydrolase-1", "journal": "Eur. J. Biochem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10866813/" }, { "id": "PMID:17502665", "title": "Adenosine generation catalyzed by CD39 and CD73 expressed on regulatory T cells mediates immune suppression", "journal": "J. Exp. Med.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17502665/" }, { "id": "PMID:18404504", "title": "Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8", "journal": "Purinergic Signal.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/18404504/" }, { "id": "PMID:21586362", "title": "Impact of ectoenzymes on p2 and p1 receptor signaling", "journal": "Adv. Pharmacol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21586362/" } ], "x": 2660, "y": 3138.6071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8850854", "label": "NTPDase1 hydrolyzes nucleoside diphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:36800" ], "source_url": "https://reactome.org/content/detail/R-HSA-8850854", "summary": "NTPDase1 hydrolyzes nucleoside diphosphates is a Reactome release 97 curated reaction event. The source record connects NDP, H2O to NMP, Pi, H+ in plasma membrane · extracellular region and cross-references RHEA:36800.", "equation": "NDP + H2O → NMP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8870906", "reactome_id": "R-ALL-8870906", "label": "NDP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8870962", "reactome_id": "R-ALL-8870962", "label": "NMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58043", "formula": "C5H8O7PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate phosphatase activity of NTPDase1:Ca2+,Mg2+ [plasma membrane]", "activity_id": "8850852", "ec_numbers": [ "3.6.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:8955160", "title": "Identification and characterization of CD39/vascular ATP diphosphohydrolase", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8955160/" }, { "id": "PMID:10866813", "title": "Distribution, cloning, and characterization of porcine nucleoside triphosphate diphosphohydrolase-1", "journal": "Eur. J. Biochem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10866813/" }, { "id": "PMID:18404504", "title": "Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8", "journal": "Purinergic Signal.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/18404504/" }, { "id": "PMID:10470077", "title": "Targeted disruption of cd39/ATP diphosphohydrolase results in disordered hemostasis and thromboregulation", "journal": "Nat. Med.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10470077/" }, { "id": "PMID:14675084", "title": "Heterologous cell-cell interactions: thromboregulation, cerebroprotection and cardioprotection by CD39 (NTPDase-1)", "journal": "J. Thromb. Haemost.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14675084/" } ], "x": 2938.666666666667, "y": 3138.6071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851089", "label": "NTPDase2 hydrolyzes nucleoside triphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:13066", "RHEA:19670", "RHEA:29388", "RHEA:28331", "RHEA:64901" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851089", "summary": "NTPDase2 hydrolyzes nucleoside triphosphates is a Reactome release 97 curated reaction event. The source record connects ATP,GTP,CTP,ITP,UTP, H2O to ADP,GDP,CDP,IDP,UDP, Pi, H+ in extracellular region · plasma membrane and cross-references RHEA:13066, RHEA:19670, RHEA:29388, RHEA:28331, RHEA:64901.", "equation": "ATP,GTP,CTP,ITP,UTP + H2O → ADP,GDP,CDP,IDP,UDP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8936166", "reactome_id": "R-ALL-8936166", "label": "ATP,GTP,CTP,ITP,UTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8851092", "reactome_id": "R-ALL-8851092", "label": "ADP,GDP,CDP,IDP,UDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside triphosphate phosphatase activity of NTPDase2:Ca2+,Mg2+ [plasma membrane]", "activity_id": "8851096", "ec_numbers": [ "3.6.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:10510450", "title": "Functional expression of a cDNA encoding a human ecto-ATPase", "journal": "Br. 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Chem.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/8995405/" }, { "id": "PMID:9364474", "title": "An ecto-ATPase and an ecto-ATP diphosphohydrolase are expressed in rat brain", "journal": "Neuropharmacology", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9364474/" }, { "id": "PMID:11929769", "title": "Differential catalytic properties and vascular topography of murine nucleoside triphosphate diphosphohydrolase 1 (NTPDase1) and NTPDase2 have implications for thromboregulation", "journal": "Blood", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11929769/" } ], "x": 2660, "y": 3053.1785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851110", "label": "NTPDase3 hydrolyzes nucleoside triphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:23681" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851110", "summary": "NTPDase3 hydrolyzes nucleoside triphosphates is a Reactome release 97 curated reaction event. The source record connects NTP, H2O to NDP, Pi, H+ in extracellular region · plasma membrane and cross-references RHEA:23681.", "equation": "NTP + H2O → NDP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8870907", "reactome_id": "R-ALL-8870907", "label": "NTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61557", "formula": "C5H8O13P3R", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8870906", "reactome_id": "R-ALL-8870906", "label": "NDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside triphosphate phosphatase activity of NTPDase3:Ca2+,Mg2+ [plasma membrane]", "activity_id": "8851106", "ec_numbers": [ "3.6.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:9675246", "title": "Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1", "journal": "Biochim. Biophys. Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9675246/" }, { "id": "PMID:18404504", "title": "Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8", "journal": "Purinergic Signal.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/18404504/" }, { "id": "PMID:15130768", "title": "Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-3", "journal": "Biochem. Pharmacol.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15130768/" }, { "id": "PMID:20682839", "title": "Identification of the ectonucleotidases expressed in mouse, rat, and human Langerhans islets: potential role of NTPDase3 in insulin secretion", "journal": "Am. J. Physiol. Endocrinol. Metab.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20682839/" }, { "id": "PMID:16338080", "title": "Immunolocalization of ecto-nucleoside triphosphate diphosphohydrolase 3 in rat brain: implications for modulation of multiple homeostatic systems including feeding and sleep-wake behaviors", "journal": "Neuroscience", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16338080/" } ], "x": 2381.3333333333335, "y": 3145.7261904761904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851129", "label": "NTPDase3 hydrolyzes nucleoside diphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:36800" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851129", "summary": "NTPDase3 hydrolyzes nucleoside diphosphates is a Reactome release 97 curated reaction event. The source record connects NDP, H2O to NMP, Pi, H+ in extracellular region · plasma membrane and cross-references RHEA:36800.", "equation": "NDP + H2O → NMP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8870906", "reactome_id": "R-ALL-8870906", "label": "NDP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8870962", "reactome_id": "R-ALL-8870962", "label": "NMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58043", "formula": "C5H8O7PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate phosphatase activity of NTPDase3:Ca2+,Mg2+ [plasma membrane]", "activity_id": "8851126", "ec_numbers": [ "3.6.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:9675246", "title": "Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1", "journal": "Biochim. Biophys. Acta", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9675246/" }, { "id": "PMID:18404504", "title": "Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8", "journal": "Purinergic Signal.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/18404504/" }, { "id": "PMID:15130768", "title": "Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-3", "journal": "Biochem. Pharmacol.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15130768/" }, { "id": "PMID:18404510", "title": "Cloning and characterization of the ecto-nucleotidase NTPDase3 from rat brain: Predicted secondary structure and relation to other members of the E-NTPDase family and actin", "journal": "Purinergic Signal.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/18404510/" } ], "x": 2660, "y": 3145.7261904761904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851225", "label": "NTPDase4 hydrolyzes nucleoside diphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "Golgi lumen · Golgi membrane", "compartment_ids": [ "GO:0005796", "GO:0000139" ], "rhea_ids": [ "RHEA:64877", "RHEA:64881", "RHEA:22157", "RHEA:64961" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851225", "summary": "NTPDase4 hydrolyzes nucleoside diphosphates is a Reactome release 97 curated reaction event. The source record connects UDP,GDP,CDP,dTDP, H2O to UMP,GMP,CMP,dTMP, Pi, H+ in Golgi lumen · Golgi membrane and cross-references RHEA:64877, RHEA:64881, RHEA:22157, RHEA:64961.", "equation": "UDP,GDP,CDP,dTDP + H2O → UMP,GMP,CMP,dTMP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8851224", "reactome_id": "R-ALL-8851224", "label": "UDP,GDP,CDP,dTDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-2022884", "reactome_id": "R-ALL-2022884", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8851227", "reactome_id": "R-ALL-8851227", "label": "UMP,GMP,CMP,dTMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-8851226", "reactome_id": "R-ALL-8851226", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate phosphatase activity of NTPDase4:Ca2+ [Golgi membrane]", "activity_id": "8851217", "ec_numbers": [ "3.6.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:9556635", "title": "Golgi localization and functional expression of human uridine diphosphatase", "journal": "J. Biol. Chem.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9556635/" } ], "x": 2660, "y": 3067.4166666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851234", "label": "NTPDase4 hydrolyzes nucleoside triphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "Golgi lumen · Golgi membrane", "compartment_ids": [ "GO:0005796", "GO:0000139" ], "rhea_ids": [ "RHEA:64901", "RHEA:29388", "RHEA:19670", "RHEA:19014" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851234", "summary": "NTPDase4 hydrolyzes nucleoside triphosphates is a Reactome release 97 curated reaction event. The source record connects UTP,GTP,CTP,dTTP, H2O to UDP,GDP,CDP,dTDP, Pi, H+ in Golgi lumen · Golgi membrane and cross-references RHEA:64901, RHEA:29388, RHEA:19670, RHEA:19014.", "equation": "UTP,GTP,CTP,dTTP + H2O → UDP,GDP,CDP,dTDP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8851233", "reactome_id": "R-ALL-8851233", "label": "UTP,GTP,CTP,dTTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-2022884", "reactome_id": "R-ALL-2022884", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8851224", "reactome_id": "R-ALL-8851224", "label": "UDP,GDP,CDP,dTDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-8851226", "reactome_id": "R-ALL-8851226", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside triphosphate phosphatase activity of NTPDase4:Ca2+ [Golgi membrane]", "activity_id": "8851236", "ec_numbers": [ "3.6.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:9556635", "title": "Golgi localization and functional expression of human uridine diphosphatase", "journal": "J. Biol. Chem.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9556635/" } ], "x": 2938.666666666667, "y": 3067.4166666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851356", "label": "NTPDase5 hydrolyzes nucleoside diphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [ "RHEA:64877", "RHEA:22157", "RHEA:64881", "RHEA:61437" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851356", "summary": "NTPDase5 hydrolyzes nucleoside diphosphates is a Reactome release 97 curated reaction event. The source record connects ADP,GDP,CDP,UDP, H2O to AMP,GMP,CMP,UMP, Pi, H+ in extracellular region and cross-references RHEA:64877, RHEA:22157, RHEA:64881, RHEA:61437.", "equation": "ADP,GDP,CDP,UDP + H2O → AMP,GMP,CMP,UMP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8936165", "reactome_id": "R-ALL-8936165", "label": "ADP,GDP,CDP,UDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8851369", "reactome_id": "R-ALL-8851369", "label": "AMP,GMP,CMP,UMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate phosphatase activity of NPTDase5:Ca2+,Mg2+ [extracellular region]", "activity_id": "8851370", "ec_numbers": [ "3.6.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:10400613", "title": "CD39-L4 is a secreted human apyrase, specific for the hydrolysis of nucleoside diphosphates", "journal": "J. Biol. Chem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10400613/" }, { "id": "PMID:11041857", "title": "Biochemical characterization of CD39L4", "journal": "Biochemistry", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11041857/" }, { "id": "PMID:21074248", "title": "The ER UDPase ENTPD5 promotes protein N-glycosylation, the Warburg effect, and proliferation in the PTEN pathway", "journal": "Cell", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21074248/" }, { "id": "PMID:22169232", "title": "ENTPD5, an endoplasmic reticulum UDPase, alleviates ER stress induced by protein overloading in AKT-activated cancer cells", "journal": "Cold Spring Harb. Symp. Quant. Biol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/22169232/" } ], "x": 2381.3333333333335, "y": 3074.535714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851396", "label": "NTPDase6 hydrolyzes nucleoside diphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [ "RHEA:22157", "RHEA:35208", "RHEA:64877", "RHEA:64881" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851396", "summary": "NTPDase6 hydrolyzes nucleoside diphosphates is a Reactome release 97 curated reaction event. The source record connects CDP,GDP,IDP,UDP, H2O to CMP,GMP,IMP,UMP, Pi, H+ in extracellular region and cross-references RHEA:22157, RHEA:35208, RHEA:64877, RHEA:64881.", "equation": "CDP,GDP,IDP,UDP + H2O → CMP,GMP,IMP,UMP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8851462", "reactome_id": "R-ALL-8851462", "label": "CDP,GDP,IDP,UDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8851461", "reactome_id": "R-ALL-8851461", "label": "CMP,GMP,IMP,UMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate phosphatase activity of NTPDase6:Ca2+,Mg2+ [extracellular region]", "activity_id": "8851466", "ec_numbers": [ "3.6.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:11041856", "title": "CD39L2, a gene encoding a human nucleoside diphosphatase, predominantly expressed in the heart", "journal": "Biochemistry", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11041856/" }, { "id": "PMID:14529283", "title": "Bacterial expression, characterization, and disulfide bond determination of soluble human NTPDase6 (CD39L2) nucleotidase: implications for structure and function", "journal": "Biochemistry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14529283/" }, { "id": "PMID:10948193", "title": "Expression and characterization of soluble and membrane-bound human nucleoside triphosphate diphosphohydrolase 6 (CD39L2)", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10948193/" } ], "x": 2660, "y": 3074.535714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851494", "label": "NTPDase7 hydrolyzes nucleoside triphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "endocytic vesicle lumen · endocytic vesicle membrane", "compartment_ids": [ "GO:0071682", "GO:0030666" ], "rhea_ids": [ "RHEA:29388", "RHEA:64901", "RHEA:19670" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851494", "summary": "NTPDase7 hydrolyzes nucleoside triphosphates is a Reactome release 97 curated reaction event. The source record connects CTP,GTP,UTP, H2O to CDP,GDP,UDP, Pi, H+ in endocytic vesicle lumen · endocytic vesicle membrane and cross-references RHEA:29388, RHEA:64901, RHEA:19670.", "equation": "CTP,GTP,UTP + H2O → CDP,GDP,UDP + Pi + H+", "inputs": [ { "node_id": "R-ALL-8851509", "reactome_id": "R-ALL-8851509", "label": "CTP,GTP,UTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endocytic vesicle lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-8851517", "reactome_id": "R-ALL-8851517", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endocytic vesicle lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8851518", "reactome_id": "R-ALL-8851518", "label": "CDP,GDP,UDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endocytic vesicle lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-8851513", "reactome_id": "R-ALL-8851513", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "endocytic vesicle lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5228597", "reactome_id": "R-ALL-5228597", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endocytic vesicle lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside triphosphate phosphatase activity of NTPDase7:Ca2+ [endocytic vesicle membrane]", "activity_id": "8851507", "ec_numbers": [ "3.6.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:11278936", "title": "Molecular cloning and characterization of a novel mammalian endo-apyrase (LALP1)", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11278936/" }, { "id": "PMID:23241884", "title": "Ecto-nucleoside triphosphate diphosphohydrolase 7 controls Th17 cell responses through regulation of luminal ATP in the small intestine", "journal": "J. Immunol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23241884/" } ], "x": 2660, "y": 3046.059523809524, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851538", "label": "NTPDase8 hydrolyzes nucleoside triphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:13066", "RHEA:19670", "RHEA:28331", "RHEA:29388", "RHEA:19014", "RHEA:64901" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851538", "summary": "NTPDase8 hydrolyzes nucleoside triphosphates is a Reactome release 97 curated reaction event. The source record connects H2O, ATP,GTP,ITP,CTP,TTP,UTP to Pi, ADP,GDP,IDP,CDP,TDP,UDP, H+ in extracellular region · plasma membrane and cross-references RHEA:13066, RHEA:19670, RHEA:28331, RHEA:29388, RHEA:19014, RHEA:64901.", "equation": "H2O + ATP,GTP,ITP,CTP,TTP,UTP → Pi + ADP,GDP,IDP,CDP,TDP,UDP + H+", "inputs": [ { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9726184", "reactome_id": "R-ALL-9726184", "label": "ATP,GTP,ITP,CTP,TTP,UTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9726189", "reactome_id": "R-ALL-9726189", "label": "ADP,GDP,IDP,CDP,TDP,UDP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside triphosphate phosphatase activity of NTPDase8:Ca2+,Mg2+ [plasma membrane]", "activity_id": "8851543", "ec_numbers": [ "3.6.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:17095758", "title": "Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8", "journal": "Am. J. Physiol. Gastrointest. Liver Physiol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17095758/" }, { "id": "PMID:10681547", "title": "Identification and characterization of a novel hepatic canalicular ATP diphosphohydrolase", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681547/" } ], "x": 2938.666666666667, "y": 3046.059523809524, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8851550", "label": "NTPDase8 hydrolyzes nucleoside diphosphates", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:64961", "RHEA:61437", "RHEA:22157", "RHEA:35208", "RHEA:64881", "RHEA:64877" ], "source_url": "https://reactome.org/content/detail/R-HSA-8851550", "summary": "NTPDase8 hydrolyzes nucleoside diphosphates is a Reactome release 97 curated reaction event. The source record connects H2O, ADP,GDP,IDP,CDP,TDP,UDP to Pi, AMP,GMP,IMP,CMP,TMP,UMP, H+ in extracellular region · plasma membrane and cross-references RHEA:64961, RHEA:61437, RHEA:22157, RHEA:35208, RHEA:64881, RHEA:64877.", "equation": "H2O + ADP,GDP,IDP,CDP,TDP,UDP → Pi + AMP,GMP,IMP,CMP,TMP,UMP + H+", "inputs": [ { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9726189", "reactome_id": "R-ALL-9726189", "label": "ADP,GDP,IDP,CDP,TDP,UDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9726186", "reactome_id": "R-ALL-9726186", "label": "AMP,GMP,IMP,CMP,TMP,UMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate phosphatase activity of NTPDase8:Ca2+,Mg2+ [plasma membrane]", "activity_id": "8851544", "ec_numbers": [ "3.6.1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:17095758", "title": "Cloning, purification, and identification of the liver canalicular ecto-ATPase as NTPDase8", "journal": "Am. J. Physiol. Gastrointest. Liver Physiol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17095758/" }, { "id": "PMID:10681547", "title": "Identification and characterization of a novel hepatic canalicular ATP diphosphohydrolase", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10681547/" }, { "id": "PMID:15122917", "title": "Cloning and characterization of mouse nucleoside triphosphate diphosphohydrolase-8", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15122917/" } ], "x": 2381.3333333333335, "y": 3046.059523809524, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8853391", "label": "GlcNAc (Man)5 translocates from cytosol to lysosomal lumen", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "oligosaccharide-recovery" ], "compartment": "cytosol · lysosomal lumen", "compartment_ids": [ "GO:0005829", "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8853391", "summary": "GlcNAc (Man)5 translocates from cytosol to lysosomal lumen is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc to alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc in cytosol · lysosomal lumen.", "equation": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc → alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc", "inputs": [ { "node_id": "R-ALL-6799543", "reactome_id": "R-ALL-6799543", "label": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:91280", "formula": "C38H65NO31", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8853396", "reactome_id": "R-ALL-8853396", "label": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:91280", "formula": "C38H65NO31", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9008702", "title": "Transfer of free polymannose-type oligosaccharides from the cytosol to lysosomes in cultured human hepatocellular carcinoma HepG2 cells", "journal": "J. Cell Biol.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9008702/" } ], "x": 3600, "y": 1033.75, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-8853686", "label": "MAN2B1 hydrolyses GlcNAc (Man)5 to GlcNAc (Man)3", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "oligosaccharide-recovery" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8853686", "summary": "MAN2B1 hydrolyses GlcNAc (Man)5 to GlcNAc (Man)3 is a Reactome release 97 curated reaction event. The source record connects alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc, H2O to alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc, Man in lysosomal lumen.", "equation": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc + H2O → alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc + 2 Man", "inputs": [ { "node_id": "R-ALL-8853396", "reactome_id": "R-ALL-8853396", "label": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:91280", "formula": "C38H65NO31", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-6799577", "reactome_id": "R-ALL-6799577", "label": "alpha-D-Man-(1-\u003e3)-[alpha-D-Man-(1-\u003e6)]-beta-D-Man-(1-\u003e4)-beta-D-GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:91279", "formula": "C26H45NO21", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-6799594", "reactome_id": "R-ALL-6799594", "label": "Man", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:4208", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alpha-mannosidase activity of MAN2B1:Zn2+ [lysosomal lumen]", "activity_id": "8853698", "ec_numbers": [ "3.2.1.24" ], "accessions": [] } ], "citations": [ { "id": "PMID:8910458", "title": "Cloning, expression, purification, and characterization of the human broad specificity lysosomal acid alpha-mannosidase", "journal": "J. Biol. Chem.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8910458/" }, { "id": "PMID:11350179", "title": "Purification and characterization of recombinant human lysosomal alpha-mannosidase", "journal": "Mol. Genet. Metab.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11350179/" }, { "id": "PMID:10513892", "title": "Lysosomal alpha-D-mannosidase", "journal": "Biosci. Rep.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10513892/" }, { "id": "PMID:25762455", "title": "amamutdb.no: A relational database for MAN2B1 allelic variants that compiles genotypes, clinical phenotypes, and biochemical and structural data of mutant MAN2B1 in α-mannosidosis", "journal": "Hum. Mutat.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25762455/" }, { "id": "PMID:25345101", "title": "Alpha-mannosidosis - a review of genetic, clinical findings and options of treatment", "journal": "Pediatr Endocrinol Rev", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25345101/" } ], "x": 3878.666666666667, "y": 839.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8853710", "label": "MANBA hydrolyses GlcNAc:Man", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "oligosaccharide-recovery" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8853710", "summary": "MANBA hydrolyses GlcNAc:Man is a Reactome release 97 curated reaction event. The source record connects GlcNAc:Man to Man, GlcNAc in lysosomal lumen.", "equation": "GlcNAc:Man → Man + GlcNAc", "inputs": [ { "node_id": "R-ALL-8853719", "reactome_id": "R-ALL-8853719", "label": "GlcNAc:Man", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:71553", "formula": "C14H25NO11", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6799594", "reactome_id": "R-ALL-6799594", "label": "Man", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4208", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1678743", "reactome_id": "R-ALL-1678743", "label": "GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17411", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-mannosidase activity of MANBA [lysosomal lumen]", "activity_id": "8853695", "ec_numbers": [ "3.2.1.25" ], "accessions": [ "O00462" ] } ], "citations": [ { "id": "PMID:9384606", "title": "Human beta-mannosidase cDNA characterization and first identification of a mutation associated with human beta-mannosidosis", "journal": "Hum. Mol. Genet.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9384606/" }, { "id": "PMID:1576208", "title": "Mammalian beta-D-mannosidase and beta-mannosidosis", "journal": "Biochimie", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1576208/" }, { "id": "PMID:17420068", "title": "Angiokeratoma corporis diffusum in human beta-mannosidosis: Report of a new case and a novel mutation", "journal": "J. Am. Acad. Dermatol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17420068/" } ], "x": 3321.3333333333335, "y": 1033.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8856223", "label": "B4GALNT1 dimer transfers GalNAc to gangliosides", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8856223", "summary": "B4GALNT1 dimer transfers GalNAc to gangliosides is a Reactome release 97 curated reaction event. The source record connects GD3,GM3,GT3,SM3,LacCer, UDP-GalNAc to GD2,GM2, GA2,GT2,SM2, UDP, H+ in Golgi membrane · Golgi lumen.", "equation": "GD3,GM3,GT3,SM3,LacCer + UDP-GalNAc → GD2,GM2, GA2,GT2,SM2 + UDP + H+", "inputs": [ { "node_id": "R-ALL-8856243", "reactome_id": "R-ALL-8856243", "label": "GD3,GM3,GT3,SM3,LacCer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-735700", "reactome_id": "R-ALL-735700", "label": "UDP-GalNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57847", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8856242", "reactome_id": "R-ALL-8856242", "label": "GD2,GM2, GA2,GT2,SM2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase activity of B4GALNT1 dimer [Golgi membrane]", "activity_id": "8856222", "ec_numbers": [ "2.4.1.92" ], "accessions": [] } ], "citations": [ { "id": "PMID:11018043", "title": "Disulfide bonds of GM2 synthase homodimers. Antiparallel orientation of the catalytic domains", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11018043/" }, { "id": "PMID:23746551", "title": "Alteration of ganglioside biosynthesis responsible for complex hereditary spastic paraplegia", "journal": "Am. J. Hum. Genet.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23746551/" }, { "id": "PMID:1601877", "title": "Expression cloning of beta 1,4 N-acetylgalactosaminyltransferase cDNAs that determine the expression of GM2 and GD2 gangliosides", "journal": "J. Biol. Chem.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1601877/" }, { "id": "PMID:7890749", "title": "Substrate specificity of beta 1,4-N-acetylgalactosaminyltransferase in vitro and in cDNA-transfected cells. GM2/GD2 synthase efficiently generates asialo-GM2 in certain cells", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7890749/" }, { "id": "PMID:29802621", "title": "Emerging concepts of ganglioside metabolism", "journal": "FEBS Lett", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29802621/" } ], "x": 3321.3333333333335, "y": 1794.826923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8858298", "label": "HRASLS transfer acyl group from PC to PE to form NAPE", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8858298", "summary": "HRASLS transfer acyl group from PC to PE to form NAPE is a Reactome release 97 curated reaction event. The source record connects PC, PE to LysoPtdCho, NAPE in cytosol · endoplasmic reticulum membrane.", "equation": "PC + PE → LysoPtdCho + NAPE", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500581", "reactome_id": "R-ALL-1500581", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6814253", "reactome_id": "R-ALL-6814253", "label": "LysoPtdCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58168", "formula": "C9H19NO7PR", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5694568", "reactome_id": "R-ALL-5694568", "label": "NAPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61232", "formula": "C8H11NO9PR3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyltransferase activity of HRASLS [cytosol]", "activity_id": "8858543", "ec_numbers": [ "2.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:26503625", "title": "The HRASLS (PLA/AT) subfamily of enzymes", "journal": "J. Biomed. Sci.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26503625/" }, { "id": "PMID:22605381", "title": "Structural basis for the acyltransferase activity of lecithin:retinol acyltransferase-like proteins", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22605381/" }, { "id": "PMID:22825852", "title": "Generation of N-acylphosphatidylethanolamine by members of the phospholipase A/acyltransferase (PLA/AT) family", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22825852/" } ], "x": 2938.666666666667, "y": 1834.0753424657535, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8862137", "label": "HSD17B2 oxidises estradiol (EST17b) to estrone (E1)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:24617" ], "source_url": "https://reactome.org/content/detail/R-HSA-8862137", "summary": "HSD17B2 oxidises estradiol (EST17b) to estrone (E1) is a Reactome release 97 curated reaction event. The source record connects NADP+, EST17b to E1, NADPH, H+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:24617.", "equation": "NADP+ + EST17b → E1 + NADPH + H+", "inputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193132", "reactome_id": "R-ALL-193132", "label": "EST17b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16469", "formula": "C18H24O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193066", "reactome_id": "R-ALL-193066", "label": "E1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17263", "formula": "C18H22O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "estradiol 17-beta-dehydrogenase [NAD(P)+] activity of HSD17B2 [endoplasmic reticulum membrane]", "activity_id": "8862138", "ec_numbers": [ "1.1.1.62" ], "accessions": [ "P37059" ] } ], "citations": [ { "id": "PMID:8099587", "title": "Expression cloning and characterization of human 17 beta-hydroxysteroid dehydrogenase type 2, a microsomal enzyme possessing 20 alpha-hydroxysteroid dehydrogenase activity", "journal": "J. Biol. Chem.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8099587/" }, { "id": "PMID:26230928", "title": "17β-Hydroxysteroid Dehydrogenase Type 2 Inhibition: Discovery of Selective and Metabolically Stable Compounds Inhibiting Both the Human Enzyme and Its Murine Ortholog", "journal": "PLoS ONE", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26230928/" }, { "id": "PMID:9783542", "title": "17Beta-hydroxysteroid dehydrogenases in human bone cells", "journal": "J. Bone Miner. Res.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9783542/" } ], "x": 4730, "y": 1618.4621212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8862152", "label": "2xHSD17B8:2xCBR4 reduces 3OA-ACP to 3HA-ACP", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:17399" ], "source_url": "https://reactome.org/content/detail/R-HSA-8862152", "summary": "2xHSD17B8:2xCBR4 reduces 3OA-ACP to 3HA-ACP is a Reactome release 97 curated reaction event. The source record connects NADPH, H+, 3OA-ACP to NADP+, 3HA-ACP in mitochondrial matrix and cross-references RHEA:17399.", "equation": "NADPH + H+ + 3OA-ACP → NADP+ + 3HA-ACP", "inputs": [ { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8862377", "reactome_id": "R-ALL-8862377", "label": "3OA-ACP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78776", "formula": "C17H26N3O10PSR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8862371", "reactome_id": "R-ALL-8862371", "label": "3HA-ACP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78827", "formula": "C17H28N3O10PSR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity of 2xHSD17B8:2xCBR4 [mitochondrial matrix]", "activity_id": "8862163", "ec_numbers": [ "1.1.1.100" ], "accessions": [] } ], "citations": [ { "id": "PMID:17978863", "title": "Expression in E. coli and tissue distribution of the human homologue of the mouse Ke 6 gene, 17beta-hydroxysteroid dehydrogenase type 8", "journal": "Mol. Cell. Biochem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17978863/" }, { "id": "PMID:19571038", "title": "17beta-hydroxysteroid dehydrogenase type 8 and carbonyl reductase type 4 assemble as a ketoacyl reductase of human mitochondrial FAS", "journal": "FASEB J.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19571038/" }, { "id": "PMID:15668256", "title": "Cloning, expression, and characterization of the human mitochondrial beta-ketoacyl synthase. Complementation of the yeast CEM1 knock-out strain", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15668256/" }, { "id": "PMID:25203508", "title": "Insights into mitochondrial fatty acid synthesis from the structure of heterotetrameric 3-ketoacyl-ACP reductase/3R-hydroxyacyl-CoA dehydrogenase", "journal": "Nat Commun", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25203508/" } ], "x": 868.6666666666667, "y": 1673.3157894736842, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8863761", "label": "UXS1 tetramer decarboxylates UDP-glucuronate to UDP-xylose", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "biological-oxidations", "glycosaminoglycans", "phase-two" ], "compartment": "Golgi lumen · Golgi membrane", "compartment_ids": [ "GO:0005796", "GO:0000139" ], "rhea_ids": [ "RHEA:23917" ], "source_url": "https://reactome.org/content/detail/R-HSA-8863761", "summary": "UXS1 tetramer decarboxylates UDP-glucuronate to UDP-xylose is a Reactome release 97 curated reaction event. The source record connects UDP-GlcA, H+ to UDP-Xyl, CO2 in Golgi lumen · Golgi membrane and cross-references RHEA:23917.", "equation": "UDP-GlcA + H+ → UDP-Xyl + CO2", "inputs": [ { "node_id": "R-ALL-1889970", "reactome_id": "R-ALL-1889970", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-742361", "reactome_id": "R-ALL-742361", "label": "UDP-Xyl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57632", "formula": "C14H20N2O16P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8863760", "reactome_id": "R-ALL-8863760", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "UDP-glucuronate decarboxylase activity of UXS1 tetramer [Golgi membrane]", "activity_id": "8863767", "ec_numbers": [ "4.1.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:22810237", "title": "Structure and mechanism of human UDP-xylose synthase: evidence for a promoting role of sugar ring distortion in a three-step catalytic conversion of UDP-glucuronic acid", "journal": "J Biol Chem", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22810237/" }, { "id": "PMID:11877387", "title": "UDP-glucuronate decarboxylase, a key enzyme in proteoglycan synthesis: cloning, characterization, and localization", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11877387/" }, { "id": "PMID:23656592", "title": "Human UDP-α-d-xylose synthase forms a catalytically important tetramer that has not been observed in crystal structures", "journal": "Biochemistry", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23656592/" }, { "id": "PMID:25521717", "title": "Man o' war mutation in UDP-α-D-xylose synthase favors the abortive catalytic cycle and uncovers a latent potential for hexamer formation", "journal": "Biochemistry", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25521717/" } ], "x": 2938.666666666667, "y": 759.6818181818181, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8865637", "label": "MFSD2A transports LPC from extracellular region to plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane · cytosol", "compartment_ids": [ "GO:0005576", "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8865637", "summary": "MFSD2A transports LPC from extracellular region to plasma membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects LPC (22:6), Na+ to LPC (22:6), Na+ in extracellular region · plasma membrane · cytosol.", "equation": "LPC (22:6) + Na+ → LPC (22:6) + Na+", "inputs": [ { "node_id": "R-ALL-8865642", "reactome_id": "R-ALL-8865642", "label": "LPC (22:6)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64567", "formula": "C30H50NO7P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-74113", "reactome_id": "R-ALL-74113", "label": "Na+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8865629", "reactome_id": "R-ALL-8865629", "label": "LPC (22:6)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64567", "formula": "C30H50NO7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-83910", "reactome_id": "R-ALL-83910", "label": "Na+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29101", "formula": "Na", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phospholipid transfer activity of MFSD2A [plasma membrane]", "activity_id": "8865644", "ec_numbers": null, "accessions": [ "Q8NA29" ] } ], "citations": [ { "id": "PMID:26005868", "title": "Inactivating mutations in MFSD2A, required for omega-3 fatty acid transport in brain, cause a lethal microcephaly syndrome", "journal": "Nat. Genet.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26005868/" }, { "id": "PMID:26945070", "title": "Structural insights into the transport mechanism of the human sodium-dependent lysophosphatidylcholine transporter Mfsd2a", "journal": "J. Biol. Chem.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26945070/" }, { "id": "PMID:24828044", "title": "Mfsd2a is a transporter for the essential omega-3 fatty acid docosahexaenoic acid", "journal": "Nature", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24828044/" } ], "x": 2381.3333333333335, "y": 1839.4041095890411, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8866405", "label": "RNR (M1M2B) reduces nucleotide diphosphates to deoxynucleotide diphosphates (glutaredoxin)", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8866405", "summary": "RNR (M1M2B) reduces nucleotide diphosphates to deoxynucleotide diphosphates (glutaredoxin) is a Reactome release 97 curated reaction event. The source record connects NDP(3-), GLRX to dNDP(3-), 2xHC-GLRX, H2O in cytosol.", "equation": "NDP(3-) + GLRX → dNDP(3-) + 2xHC-GLRX + H2O", "inputs": [ { "node_id": "R-ALL-8866401", "reactome_id": "R-ALL-8866401", "label": "NDP(3-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57930", "formula": "C5H8O10P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-55729", "reactome_id": "R-HSA-55729", "label": "GLRX", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8866399", "reactome_id": "R-ALL-8866399", "label": "dNDP(3-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73316", "formula": "C5H8O9P2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-111740", "reactome_id": "R-HSA-111740", "label": "2xHC-GLRX", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribonucleoside-diphosphate reductase activity, glutaredoxin disulfide as acceptor of RNR (M1M2B) [cytosol]", "activity_id": "8866406", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11796141", "title": "Structure and function of the radical enzyme ribonucleotide reductase", "journal": "Prog Biophys Mol Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11796141/" }, { "id": "PMID:11517226", "title": "Mammalian p53R2 protein forms an active ribonucleotide reductase in vitro with the R1 protein, which is expressed both in resting cells in response to DNA damage and in proliferating cells", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11517226/" }, { "id": "PMID:2668278", "title": "Thioredoxin and glutaredoxin systems", "journal": "J Biol Chem", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2668278/" }, { "id": "PMID:18997010", "title": "Ribonucleotide reduction is a cytosolic process in mammalian cells independently of DNA damage", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18997010/" }, { "id": "PMID:22847445", "title": "Mammalian ribonucleotide reductase subunit p53R2 is required for mitochondrial DNA replication and DNA repair in quiescent cells", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22847445/" }, { "id": "PMID:10884394", "title": "Cross-talk between the allosteric effector-binding sites in mouse ribonucleotide reductase", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10884394/" } ], "x": 3321.3333333333335, "y": 3185.7708333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8866601", "label": "SAMHD1:Zn2+ tetramer hydrolyzes dNTP to nucleoside and triphosphate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [ "RHEA:46149" ], "source_url": "https://reactome.org/content/detail/R-HSA-8866601", "summary": "SAMHD1:Zn2+ tetramer hydrolyzes dNTP to nucleoside and triphosphate is a Reactome release 97 curated reaction event. The source record connects H2O, dNTP(4-) to 2'-deoxynucleoside, PPPi, H+ in nucleoplasm and cross-references RHEA:46149.", "equation": "H2O + dNTP(4-) → 2'-deoxynucleoside + PPPi + H+", "inputs": [ { "node_id": "R-ALL-113518", "reactome_id": "R-ALL-113518", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8866574", "reactome_id": "R-ALL-8866574", "label": "dNTP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61560", "formula": "C5H8O12P3R", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8866608", "reactome_id": "R-ALL-8866608", "label": "2'-deoxynucleoside", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18274", "formula": "C5H9O3R", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8866567", "reactome_id": "R-ALL-8866567", "label": "PPPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18036", "formula": "O10P3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9668967", "reactome_id": "R-ALL-9668967", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "triphosphoric monoester hydrolase activity of SAMHD1:Zn2+ tetramer [nucleoplasm]", "activity_id": "8866631", "ec_numbers": [ "3.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:23858451", "title": "The deoxynucleotide triphosphohydrolase SAMHD1 is a major regulator of DNA precursor pools in mammalian cells", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23858451/" }, { "id": "PMID:22056990", "title": "HIV-1 restriction factor SAMHD1 is a deoxynucleoside triphosphate triphosphohydrolase", "journal": "Nature", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/22056990/" }, { "id": "PMID:22069334", "title": "Aicardi-Goutieres syndrome gene and HIV-1 restriction factor SAMHD1 is a dGTP-regulated deoxynucleotide triphosphohydrolase", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/22069334/" }, { "id": "PMID:19525956", "title": "Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response", "journal": "Nat. Genet.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19525956/" }, { "id": "PMID:23426366", "title": "Tetramerization of SAMHD1 is required for biological activity and inhibition of HIV infection", "journal": "J. Biol. Chem.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23426366/" }, { "id": "PMID:24217394", "title": "Structural insight into dGTP-dependent activation of tetrameric SAMHD1 deoxynucleoside triphosphate triphosphohydrolase", "journal": "Nat Commun", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24217394/" } ], "x": 2938.666666666667, "y": 3145.7261904761904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8866941", "label": "MID1IP1 binds THRSP", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8866941", "summary": "MID1IP1 binds THRSP is a Reactome release 97 curated reaction event. The source record connects MID1IP1, THRSP to MID1IP1:THRSP in cytosol.", "equation": "MID1IP1 + THRSP → MID1IP1:THRSP", "inputs": [ { "node_id": "R-HSA-8867328", "reactome_id": "R-HSA-8867328", "label": "MID1IP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-8867326", "reactome_id": "R-HSA-8867326", "label": "THRSP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8867329", "reactome_id": "R-HSA-8867329", "label": "MID1IP1:THRSP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular function activator activity of THRSP [cytosol]", "activity_id": "9954170", "ec_numbers": null, "accessions": [ "Q92748" ] } ], "citations": [ { "id": "PMID:18556348", "title": "Hepatic expression of the SPOT 14 (S14) paralog S14-related (Mid1 interacting protein) is regulated by dietary carbohydrate", "journal": "Endocrinology", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18556348/" }, { "id": "PMID:20233797", "title": "Overlapping roles of the glucose-responsive genes, S14 and S14R, in hepatic lipogenesis", "journal": "Endocrinology", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20233797/" }, { "id": "PMID:24277613", "title": "Spot14/Mig12 heterocomplex sequesters polymerization and restrains catalytic function of human acetyl-CoA carboxylase 2", "journal": "J. Mol. Recognit.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24277613/" } ], "x": 590, "y": 1742.719298245614, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8867667", "label": "OSBPs transport 25OH-CHOL from ER membrane to plasma membrane", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005789", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8867667", "summary": "OSBPs transport 25OH-CHOL from ER membrane to plasma membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects 25OH-CHOL to 25OH-CHOL in cytosol · endoplasmic reticulum membrane · plasma membrane.", "equation": "25OH-CHOL → 25OH-CHOL", "inputs": [ { "node_id": "R-ALL-8868399", "reactome_id": "R-ALL-8868399", "label": "25OH-CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:42977", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8868400", "reactome_id": "R-ALL-8868400", "label": "25OH-CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:42977", "formula": "C27H46O2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sterol transfer activity of OSBPs [cytosol]", "activity_id": "8868401", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11802775", "title": "Oxysterol-binding-protein (OSBP)-related protein 4 binds 25-hydroxycholesterol and interacts with vimentin intermediate filaments", "journal": "Biochem. J.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11802775/" }, { "id": "PMID:24742681", "title": "Oxysterol-binding protein (OSBP)-related protein 4 (ORP4) is essential for cell proliferation and survival", "journal": "J. Biol. Chem.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24742681/" }, { "id": "PMID:26715851", "title": "OSBP-Related Protein Family in Lipid Transport Over Membrane Contact Sites", "journal": "Lipid Insights", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26715851/" }, { "id": "PMID:17428193", "title": "The mammalian oxysterol-binding protein-related proteins (ORPs) bind 25-hydroxycholesterol in an evolutionarily conserved pocket", "journal": "Biochem. J.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17428193/" }, { "id": "PMID:23830809", "title": "Oxysterol-binding proteins: sterol and phosphoinositide sensors coordinating transport, signaling and metabolism", "journal": "Prog. Lipid Res.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23830809/" }, { "id": "PMID:23428468", "title": "Oxysterol-binding proteins: functions in cell regulation beyond lipid metabolism", "journal": "Biochem. Pharmacol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23428468/" } ], "x": 4451.333333333333, "y": 1742.2348484848485, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8867876", "label": "OSBPL5,8,10 exchange PS with PI4P from ER membrane to plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · plasma membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005886", "GO:0005829" ], "rhea_ids": [ "RHEA:81667" ], "source_url": "https://reactome.org/content/detail/R-HSA-8867876", "summary": "OSBPL5,8,10 exchange PS with PI4P from ER membrane to plasma membrane is a Reactome release 97 curated reaction event. The source record connects PS, PI4P to PS, PI4P in endoplasmic reticulum membrane · plasma membrane · cytosol and cross-references RHEA:81667.", "equation": "PS + PI4P → PS + PI4P", "inputs": [ { "node_id": "R-ALL-1500646", "reactome_id": "R-ALL-1500646", "label": "PS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-392417", "reactome_id": "R-ALL-392417", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-33625", "reactome_id": "R-ALL-33625", "label": "PS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1806234", "reactome_id": "R-ALL-1806234", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phospholipid transfer activity of OSBPL5,8,10 [cytosol]", "activity_id": "8867867", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:23934110", "title": "Interactome map uncovers phosphatidylserine transport by oxysterol-binding proteins", "journal": "Nature", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23934110/" }, { "id": "PMID:22906437", "title": "ORP10, a cholesterol binding protein associated with microtubules, regulates apolipoprotein B-100 secretion", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22906437/" }, { "id": "PMID:19554302", "title": "OSBPL10, a novel candidate gene for high triglyceride trait in dyslipidemic Finnish subjects, regulates cellular lipid metabolism", "journal": "J. Mol. Med.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19554302/" }, { "id": "PMID:26206935", "title": "INTRACELLULAR TRANSPORT. PI4P/phosphatidylserine countertransport at ORP5- and ORP8-mediated ER-plasma membrane contacts", "journal": "Science", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26206935/" }, { "id": "PMID:21220512", "title": "A role for oxysterol-binding protein-related protein 5 in endosomal cholesterol trafficking", "journal": "J. Cell Biol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21220512/" } ], "x": 2660, "y": 1658.2260273972602, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8868402", "label": "OSBP exchanges 25OH-CHOL with PI4P from ER membrane to Golgi membrane", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane · Golgi membrane", "compartment_ids": [ "GO:0005829", "GO:0005789", "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8868402", "summary": "OSBP exchanges 25OH-CHOL with PI4P from ER membrane to Golgi membrane is a Reactome release 97 curated reaction event. The source record connects 25OH-CHOL, PI4P to 25OH-CHOL, PI4P in cytosol · endoplasmic reticulum membrane · Golgi membrane.", "equation": "25OH-CHOL + PI4P → 25OH-CHOL + PI4P", "inputs": [ { "node_id": "R-ALL-8868399", "reactome_id": "R-ALL-8868399", "label": "25OH-CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:42977", "formula": "C27H46O2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8868393", "reactome_id": "R-ALL-8868393", "label": "25OH-CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:42977", "formula": "C27H46O2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1806234", "reactome_id": "R-ALL-1806234", "label": "PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sterol transfer activity of OSBP [cytosol]", "activity_id": "8868391", "ec_numbers": null, "accessions": [ "P22059" ] } ], "citations": [ { "id": "PMID:24209621", "title": "A four-step cycle driven by PI(4)P hydrolysis directs sterol/PI(4)P exchange by the ER-Golgi tether OSBP", "journal": "Cell", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24209621/" } ], "x": 4730, "y": 1718.659090909091, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8869241", "label": "PITPNM1,2,3 exchange PI for PA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005789", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8869241", "summary": "PITPNM1,2,3 exchange PI for PA is a Reactome release 97 curated reaction event. The source record connects PI, PA to PI, PA in cytosol · endoplasmic reticulum membrane · plasma membrane.", "equation": "PI + PA → PI + PA", "inputs": [ { "node_id": "R-ALL-426639", "reactome_id": "R-ALL-426639", "label": "PI", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602448", "reactome_id": "R-ALL-1602448", "label": "PA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-426658", "reactome_id": "R-ALL-426658", "label": "PI", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57880", "formula": "C11H16O13PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-426755", "reactome_id": "R-ALL-426755", "label": "PA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol transfer activity of PITPNM1,2,3 [cytosol]", "activity_id": "8869233", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:26028218", "title": "Phosphatidylinositol-Phosphatidic Acid Exchange by Nir2 at ER-PM Contact Sites Maintains Phosphoinositide Signaling Competence", "journal": "Dev. Cell", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26028218/" }, { "id": "PMID:25887399", "title": "Phosphatidylinositol 4,5-Bisphosphate Homeostasis Regulated by Nir2 and Nir3 Proteins at Endoplasmic Reticulum-Plasma Membrane Junctions", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25887399/" }, { "id": "PMID:10022914", "title": "Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein", "journal": "Mol. Cell. Biol.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10022914/" }, { "id": "PMID:17377520", "title": "Mutation in the PYK2-binding domain of PITPNM3 causes autosomal dominant cone dystrophy (CORD5) in two Swedish families", "journal": "Eur. J. Hum. Genet.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17377520/" } ], "x": 2660, "y": 1839.4041095890411, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8869425", "label": "PLA1A hydrolyses PS to 2-acyl LPS", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8869425", "summary": "PLA1A hydrolyses PS to 2-acyl LPS is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, PS to 2-acyl LPS, LCFA(-) in extracellular region · plasma membrane.", "equation": "H2O + PS → 2-acyl LPS + LCFA(-)", "inputs": [ { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-33625", "reactome_id": "R-ALL-33625", "label": "PS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11750", "formula": "C8H12NO10PR2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8869427", "reactome_id": "R-ALL-8869427", "label": "2-acyl LPS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:37646", "formula": "C7H13NO9PR", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1602366", "reactome_id": "R-ALL-1602366", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glycerophospholipid phospholipase A1 activity of PLA1A [extracellular region]", "activity_id": "8869424", "ec_numbers": [ "3.1.1.32" ], "accessions": [ "Q53H76" ] } ], "citations": [ { "id": "PMID:10196188", "title": "An alternative splicing form of phosphatidylserine-specific phospholipase A1 that exhibits lysophosphatidylserine-specific lysophospholipase activity in humans", "journal": "J. Biol. Chem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10196188/" }, { "id": "PMID:17101204", "title": "Structure and function of extracellular phospholipase A1 belonging to the pancreatic lipase gene family", "journal": "Biochimie", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17101204/" } ], "x": 2938.666666666667, "y": 1839.4041095890411, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8869603", "label": "SLC22A13 exchanges extracellular NCA for cytosolic urate", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane · extracellular region · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005576", "GO:0005829" ], "rhea_ids": [ "RHEA:72023" ], "source_url": "https://reactome.org/content/detail/R-HSA-8869603", "summary": "SLC22A13 exchanges extracellular NCA for cytosolic urate is a Reactome release 97 curated reaction event. The source record connects NCA, urate to NCA, urate in plasma membrane · extracellular region · cytosol and cross-references RHEA:72023.", "equation": "NCA + urate → NCA + urate", "inputs": [ { "node_id": "R-ALL-8869604", "reactome_id": "R-ALL-8869604", "label": "NCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32544", "formula": "C6H4NO2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-30034", "reactome_id": "R-ALL-30034", "label": "urate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17775", "formula": "C5H4N4O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-197230", "reactome_id": "R-ALL-197230", "label": "NCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32544", "formula": "C6H4NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-429056", "reactome_id": "R-ALL-429056", "label": "urate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17775", "formula": "C5H4N4O3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinate transmembrane transporter activity of SLC22A13 [plasma membrane]", "activity_id": "8869616", "ec_numbers": null, "accessions": [ "Q9Y226" ] } ], "citations": [ { "id": "PMID:18411268", "title": "Identification of a new urate and high affinity nicotinate transporter, hOAT10 (SLC22A13)", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18411268/" } ], "x": 5391.333333333333, "y": 2869.5394736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8869606", "label": "NMRK1 phosphorylates NAR to yield NAMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25569" ], "source_url": "https://reactome.org/content/detail/R-HSA-8869606", "summary": "NMRK1 phosphorylates NAR to yield NAMN is a Reactome release 97 curated reaction event. The source record connects NAR, ATP to ADP, H+, NAMN in cytosol and cross-references RHEA:25569.", "equation": "NAR + ATP → ADP + H+ + NAMN", "inputs": [ { "node_id": "R-ALL-8869365", "reactome_id": "R-ALL-8869365", "label": "NAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58527", "formula": "C11H13NO6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8869359", "reactome_id": "R-ALL-8869359", "label": "NAMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57502", "formula": "C11H12NO9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinate riboside kinase activity of NMRK1 [cytosol]", "activity_id": "8869636", "ec_numbers": [ "2.7.1.173" ], "accessions": [ "Q9NWW6" ] } ], "citations": [ { "id": "PMID:21504897", "title": "Pathways and subcellular compartmentation of NAD biosynthesis in human cells: from entry of extracellular precursors to mitochondrial NAD generation", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21504897/" }, { "id": "PMID:19531469", "title": "The intrinsic reactivity of ATP and the catalytic proficiencies of kinases acting on glucose, N-acetylgalactosamine, and homoserine: a thermodynamic analysis", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19531469/" }, { "id": "PMID:17914902", "title": "Nicotinamide riboside kinase structures reveal new pathways to NAD+", "journal": "PLoS Biol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17914902/" } ], "x": 5391.333333333333, "y": 2838.8289473684213, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8869607", "label": "NMRK2 phosphorylates NAR to yield NAMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25569" ], "source_url": "https://reactome.org/content/detail/R-HSA-8869607", "summary": "NMRK2 phosphorylates NAR to yield NAMN is a Reactome release 97 curated reaction event. The source record connects NAR, ATP to ADP, H+, NAMN in cytosol and cross-references RHEA:25569.", "equation": "NAR + ATP → ADP + H+ + NAMN", "inputs": [ { "node_id": "R-ALL-8869365", "reactome_id": "R-ALL-8869365", "label": "NAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58527", "formula": "C11H13NO6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8869359", "reactome_id": "R-ALL-8869359", "label": "NAMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57502", "formula": "C11H12NO9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinate riboside kinase activity of NMRK2 [cytosol]", "activity_id": "8869631", "ec_numbers": [ "2.7.1.173" ], "accessions": [ "Q9NPI5" ] } ], "citations": [ { "id": "PMID:10613898", "title": "A novel muscle-specific beta 1 integrin binding protein (MIBP) that modulates myogenic differentiation", "journal": "J. Cell Biol.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10613898/" }, { "id": "PMID:21504897", "title": "Pathways and subcellular compartmentation of NAD biosynthesis in human cells: from entry of extracellular precursors to mitochondrial NAD generation", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21504897/" }, { "id": "PMID:19531469", "title": "The intrinsic reactivity of ATP and the catalytic proficiencies of kinases acting on glucose, N-acetylgalactosamine, and homoserine: a thermodynamic analysis", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19531469/" }, { "id": "PMID:17914902", "title": "Nicotinamide riboside kinase structures reveal new pathways to NAD+", "journal": "PLoS Biol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17914902/" } ], "x": 5670, "y": 2838.8289473684213, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8869627", "label": "NMRK2 phosphorylates NR to yield NMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14018" ], "source_url": "https://reactome.org/content/detail/R-HSA-8869627", "summary": "NMRK2 phosphorylates NR to yield NMN is a Reactome release 97 curated reaction event. The source record connects NR, ATP to NMN, ADP, H+ in cytosol and cross-references RHEA:14018.", "equation": "NR + ATP → NMN + ADP + H+", "inputs": [ { "node_id": "R-ALL-8869377", "reactome_id": "R-ALL-8869377", "label": "NR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15927", "formula": "C11H15N2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8869353", "reactome_id": "R-ALL-8869353", "label": "NMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:14649", "formula": "C11H14N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribosylnicotinamide kinase activity of NMRK2 [cytosol]", "activity_id": "8869614", "ec_numbers": [ "2.7.1.22" ], "accessions": [ "Q9NPI5" ] } ], "citations": [ { "id": "PMID:15137942", "title": "Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans", "journal": "Cell", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15137942/" }, { "id": "PMID:33353981", "title": "NAD + metabolism and its roles in cellular processes during ageing", "journal": "Nat Rev Mol Cell Biol", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33353981/" }, { "id": "PMID:10613898", "title": "A novel muscle-specific beta 1 integrin binding protein (MIBP) that modulates myogenic differentiation", "journal": "J. Cell Biol.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10613898/" }, { "id": "PMID:21504897", "title": "Pathways and subcellular compartmentation of NAD biosynthesis in human cells: from entry of extracellular precursors to mitochondrial NAD generation", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21504897/" }, { "id": "PMID:19531469", "title": "The intrinsic reactivity of ATP and the catalytic proficiencies of kinases acting on glucose, N-acetylgalactosamine, and homoserine: a thermodynamic analysis", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19531469/" }, { "id": "PMID:17914902", "title": "Nicotinamide riboside kinase structures reveal new pathways to NAD+", "journal": "PLoS Biol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17914902/" } ], "x": 5948.666666666667, "y": 2838.8289473684213, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8869633", "label": "NMRK1 phosphorylates NR to yield NMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14018" ], "source_url": "https://reactome.org/content/detail/R-HSA-8869633", "summary": "NMRK1 phosphorylates NR to yield NMN is a Reactome release 97 curated reaction event. The source record connects NR, ATP to NMN, ADP, H+ in cytosol and cross-references RHEA:14018.", "equation": "NR + ATP → NMN + ADP + H+", "inputs": [ { "node_id": "R-ALL-8869377", "reactome_id": "R-ALL-8869377", "label": "NR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15927", "formula": "C11H15N2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8869353", "reactome_id": "R-ALL-8869353", "label": "NMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:14649", "formula": "C11H14N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribosylnicotinamide kinase activity of NMRK1 [cytosol]", "activity_id": "8869615", "ec_numbers": [ "2.7.1.22" ], "accessions": [ "Q9NWW6" ] } ], "citations": [ { "id": "PMID:15137942", "title": "Discoveries of nicotinamide riboside as a nutrient and conserved NRK genes establish a Preiss-Handler independent route to NAD+ in fungi and humans", "journal": "Cell", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15137942/" }, { "id": "PMID:33353981", "title": "NAD + metabolism and its roles in cellular processes during ageing", "journal": "Nat Rev Mol Cell Biol", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33353981/" }, { "id": "PMID:21504897", "title": "Pathways and subcellular compartmentation of NAD biosynthesis in human cells: from entry of extracellular precursors to mitochondrial NAD generation", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21504897/" }, { "id": "PMID:8809081", "title": "Purification and properties of a human nicotinamide ribonucleoside kinase", "journal": "Arch. Biochem. Biophys.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8809081/" }, { "id": "PMID:19531469", "title": "The intrinsic reactivity of ATP and the catalytic proficiencies of kinases acting on glucose, N-acetylgalactosamine, and homoserine: a thermodynamic analysis", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19531469/" }, { "id": "PMID:17914902", "title": "Nicotinamide riboside kinase structures reveal new pathways to NAD+", "journal": "PLoS Biol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17914902/" } ], "x": 5391.333333333333, "y": 2849.065789473684, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8870332", "label": "PTPN13:PLEKHA1,2 bind PI(3,4)P2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8870332", "summary": "PTPN13:PLEKHA1,2 bind PI(3,4)P2 is a Reactome release 97 curated reaction event. The source record connects PTPN13:PLEKHA1,2, PI(3,4)P2 to PTPN13:PLEKHA1,2:PIP2 in plasma membrane.", "equation": "PTPN13:PLEKHA1,2 + PI(3,4)P2 → PTPN13:PLEKHA1,2:PIP2", "inputs": [ { "node_id": "R-HSA-8870330", "reactome_id": "R-HSA-8870330", "label": "PTPN13:PLEKHA1,2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-202247", "reactome_id": "R-ALL-202247", "label": "PI(3,4)P2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16152", "formula": "C11H19O19P3R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8870324", "reactome_id": "R-HSA-8870324", "label": "PTPN13:PLEKHA1,2:PIP2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol bisphosphate binding activity of PTPN13:PLEKHA1,2 [plasma membrane]", "activity_id": "9960147", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:14516276", "title": "Interaction of the protein tyrosine phosphatase PTPL1 with the PtdIns(3,4)P2-binding adaptor protein TAPP1", "journal": "Biochem. J.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14516276/" }, { "id": "PMID:12101241", "title": "TAPP1 and TAPP2 are targets of phosphatidylinositol 3-kinase signaling in B cells: sustained plasma membrane recruitment triggered by the B-cell antigen receptor", "journal": "Mol. Cell. Biol.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12101241/" }, { "id": "PMID:21204784", "title": "Role of TAPP1 and TAPP2 adaptor binding to PtdIns(3,4)P2 in regulating insulin sensitivity defined by knock-in analysis", "journal": "Biochem. J.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21204784/" } ], "x": 2381.3333333333335, "y": 1866.0479452054794, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8870346", "label": "BST1 hydrolyzes NAD+ to yield NAM and ADP-ribose", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:16302" ], "source_url": "https://reactome.org/content/detail/R-HSA-8870346", "summary": "BST1 hydrolyzes NAD+ to yield NAM and ADP-ribose is a Reactome release 97 curated reaction event. The source record connects NAD+, H2O to NAM, ADP-ribose, H+ in extracellular region · plasma membrane and cross-references RHEA:16302.", "equation": "NAD+ + H2O → NAM + ADP-ribose + H+", "inputs": [ { "node_id": "R-ALL-352330", "reactome_id": "R-ALL-352330", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8870343", "reactome_id": "R-ALL-8870343", "label": "NAM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8870342", "reactome_id": "R-ALL-8870342", "label": "ADP-ribose", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57967", "formula": "C15H21N5O14P2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NAD+ nucleosidase activity, cyclic ADP-ribose generating of BST1 dimer [plasma membrane]", "activity_id": "8870350", "ec_numbers": [ "3.2.2.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:11866528", "title": "Crystallographic studies on human BST-1/CD157 with ADP-ribosyl cyclase and NAD glycohydrolase activities", "journal": "J. Mol. Biol.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11866528/" } ], "x": 5670, "y": 2849.065789473684, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8870352", "label": "SLC36A4 transports L-Trp from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "plasma membrane · extracellular region · cytosol", "compartment_ids": [ "GO:0005886", "GO:0005576", "GO:0005829" ], "rhea_ids": [ "RHEA:70947" ], "source_url": "https://reactome.org/content/detail/R-HSA-8870352", "summary": "SLC36A4 transports L-Trp from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects L-Trp to L-Trp in plasma membrane · extracellular region · cytosol and cross-references RHEA:70947.", "equation": "L-Trp → L-Trp", "inputs": [ { "node_id": "R-ALL-352006", "reactome_id": "R-ALL-352006", "label": "L-Trp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57912", "formula": "C11H12N2O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29500", "reactome_id": "R-ALL-29500", "label": "L-Trp", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57912", "formula": "C11H12N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-tryptophan transmembrane transporter activity of SLC36A4 [plasma membrane]", "activity_id": "8870348", "ec_numbers": null, "accessions": [ "Q6YBV0" ] } ], "citations": [ { "id": "PMID:23478338", "title": "Rab12 regulates mTORC1 activity and autophagy through controlling the degradation of amino-acid transporter PAT4", "journal": "EMBO Rep.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23478338/" }, { "id": "PMID:21097500", "title": "SLC36A4 (hPAT4) is a high affinity amino acid transporter when expressed in Xenopus laevis oocytes", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21097500/" } ], "x": 7461.333333333333, "y": 1884.625, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8870489", "label": "PLEKHA4,(5,6) bind PI3P", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8870489", "summary": "PLEKHA4,(5,6) bind PI3P is a Reactome release 97 curated reaction event. The source record connects PLEKHA4,(5,6), PI3P to PLEKHA4,(5,6):PI3P in plasma membrane.", "equation": "PLEKHA4,(5,6) + PI3P → PLEKHA4,(5,6):PI3P", "inputs": [ { "node_id": "R-HSA-8870496", "reactome_id": "R-HSA-8870496", "label": "PLEKHA4,(5,6)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "CandidateSet", "currency": false }, { "node_id": "R-ALL-1806276", "reactome_id": "R-ALL-1806276", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8870509", "reactome_id": "R-HSA-8870509", "label": "PLEKHA4,(5,6):PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol-3-phosphate binding activity of PLEKHA4,(5,6) [plasma membrane]", "activity_id": "9960152", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:22037487", "title": "Identification and characterization of splicing variants of PLEKHA5 (Plekha5) during brain development", "journal": "Gene", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22037487/" }, { "id": "PMID:11001876", "title": "Identification of pleckstrin-homology-domain-containing proteins with novel phosphoinositide-binding specificities", "journal": "Biochem. J.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11001876/" } ], "x": 2660, "y": 1866.0479452054794, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8870499", "label": "PLEKHA3,8 bind PI4P, ARF1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "Golgi membrane", "compartment_ids": [ "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8870499", "summary": "PLEKHA3,8 bind PI4P, ARF1 is a Reactome release 97 curated reaction event. The source record connects PLEKHA3,8, PI4P, ARF1 to ARF1:PLEKHA3,8:PI4P in Golgi membrane.", "equation": "PLEKHA3,8 + PI4P + ARF1 → ARF1:PLEKHA3,8:PI4P", "inputs": [ { "node_id": "R-HSA-8870506", "reactome_id": "R-HSA-8870506", "label": "PLEKHA3,8", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-1806217", "reactome_id": "R-ALL-1806217", "label": "PI4P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17526", "formula": "C11H18O16P2R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-199971", "reactome_id": "R-HSA-199971", "label": "ARF1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8870493", "reactome_id": "R-HSA-8870493", "label": "ARF1:PLEKHA3,8:PI4P", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of PLEKHA3,8 [Golgi membrane]", "activity_id": "9960139", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15107860", "title": "FAPPs control Golgi-to-cell-surface membrane traffic by binding to ARF and PtdIns(4)P", "journal": "Nat Cell Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15107860/" }, { "id": "PMID:10559940", "title": "ARF mediates recruitment of PtdIns-4-OH kinase-beta and stimulates synthesis of PtdIns(4,5)P2 on the Golgi complex", "journal": "Nat Cell Biol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10559940/" } ], "x": 2938.666666666667, "y": 1866.0479452054794, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8871366", "label": "p-Y389,400-RUFY1 binds RAB4A:GTP, RAB5:GTP, RAB14:GTP", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane", "compartment_ids": [ "GO:0031901" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8871366", "summary": "p-Y389,400-RUFY1 binds RAB4A:GTP, RAB5:GTP, RAB14:GTP is a Reactome release 97 curated reaction event. The source record connects p-Y389,400-RUFY1, RAB14:GTP, RAB4A:GTP to p-Y389,400-RUFY1:p-Y389,400-RUFY1:RAB4A:GTP:RAB5:GTP:RAB14:GTP in early endosome membrane.", "equation": "p-Y389,400-RUFY1 + RAB14:GTP + RAB4A:GTP + RAB5A:GTP → p-Y389,400-RUFY1:p-Y389,400-RUFY1:RAB4A:GTP:RAB5:GTP:RAB14:GTP", "inputs": [ { "node_id": "R-HSA-5215951", "reactome_id": "R-HSA-5215951", "label": "p-Y389,400-RUFY1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-8871377", "reactome_id": "R-HSA-8871377", "label": "RAB14:GTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-8871367", "reactome_id": "R-HSA-8871367", "label": "RAB4A:GTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2130191", "reactome_id": "R-HSA-2130191", "label": "RAB5A:GTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8871353", "reactome_id": "R-HSA-8871353", "label": "p-Y389,400-RUFY1:p-Y389,400-RUFY1:RAB4A:GTP:RAB5:GTP:RAB14:GTP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein-macromolecule adaptor activity of p-Y389,400-RUFY1 [early endosome membrane]", "activity_id": "9960150", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:23519112", "title": "RUFY, Rab and Rap Family Proteins Involved in a Regulation of Cell Polarity and Membrane Trafficking", "journal": "Int J Mol Sci", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23519112/" }, { "id": "PMID:16154996", "title": "Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16154996/" }, { "id": "PMID:14617813", "title": "Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes", "journal": "Mol. Biol. Cell", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14617813/" }, { "id": "PMID:16522682", "title": "The Rab4 effector Rabip4 plays a role in the endocytotic trafficking of Glut 4 in 3T3-L1 adipocytes", "journal": "J. Cell. Sci.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16522682/" }, { "id": "PMID:20534812", "title": "Functional cross-talk between Rab14 and Rab4 through a dual effector, RUFY1/Rabip4", "journal": "Mol Biol Cell", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20534812/" } ], "x": 2938.666666666667, "y": 1887.36301369863, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8871370", "label": "p-Y389,400-RUFY1 translocates from cytosol to early endosome membrane", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · early endosome membrane", "compartment_ids": [ "GO:0005829", "GO:0031901" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8871370", "summary": "p-Y389,400-RUFY1 translocates from cytosol to early endosome membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects p-Y389,400-RUFY1 to p-Y389,400-RUFY1 in cytosol · early endosome membrane.", "equation": "p-Y389,400-RUFY1 → p-Y389,400-RUFY1", "inputs": [ { "node_id": "R-HSA-8871352", "reactome_id": "R-HSA-8871352", "label": "p-Y389,400-RUFY1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-5215951", "reactome_id": "R-HSA-5215951", "label": "p-Y389,400-RUFY1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11877430", "title": "Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1. A possible role of ETK in regulation of vesicle trafficking", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11877430/" } ], "x": 2381.3333333333335, "y": 1919.3356164383563, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-8871373", "label": "BMX phosphorylates RUFY1", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10597" ], "source_url": "https://reactome.org/content/detail/R-HSA-8871373", "summary": "BMX phosphorylates RUFY1 is a Reactome release 97 curated reaction event. The source record connects RUFY1, ATP to p-Y389,400-RUFY1, ADP, H+ in cytosol and cross-references RHEA:10597.", "equation": "RUFY1 + 2 ATP → p-Y389,400-RUFY1 + 2 ADP + H+", "inputs": [ { "node_id": "R-HSA-8871374", "reactome_id": "R-HSA-8871374", "label": "RUFY1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-8871352", "reactome_id": "R-HSA-8871352", "label": "p-Y389,400-RUFY1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "non-membrane spanning protein tyrosine kinase activity of BMX [cytosol]", "activity_id": "8871361", "ec_numbers": [ "2.7.10.2" ], "accessions": [ "P51813" ] } ], "citations": [ { "id": "PMID:11877430", "title": "Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1. A possible role of ETK in regulation of vesicle trafficking", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11877430/" } ], "x": 2381.3333333333335, "y": 1663.554794520548, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8871376", "label": "p-Y389,400-RUFY1 binds PI3P", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "early endosome membrane", "compartment_ids": [ "GO:0031901" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8871376", "summary": "p-Y389,400-RUFY1 binds PI3P is a Reactome release 97 curated reaction event. The source record connects p-Y389,400-RUFY1, PI3P to p-Y389,400-RUFY1:PI3P in early endosome membrane.", "equation": "p-Y389,400-RUFY1 + PI3P → p-Y389,400-RUFY1:PI3P", "inputs": [ { "node_id": "R-HSA-5215951", "reactome_id": "R-HSA-5215951", "label": "p-Y389,400-RUFY1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-1806200", "reactome_id": "R-ALL-1806200", "label": "PI3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58088", "formula": "C11H15O16P2R2", "compartment": "early endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8871354", "reactome_id": "R-HSA-8871354", "label": "p-Y389,400-RUFY1:PI3P", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "early endosome membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein-macromolecule adaptor activity of p-Y389,400-RUFY1 [early endosome membrane]", "activity_id": "9960150", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11877430", "title": "Interaction between tyrosine kinase Etk and a RUN domain- and FYVE domain-containing protein RUFY1. A possible role of ETK in regulation of vesicle trafficking", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11877430/" } ], "x": 2381.3333333333335, "y": 1871.376712328767, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8873794", "label": "PCTP binds PC", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8873794", "summary": "PCTP binds PC is a Reactome release 97 curated reaction event. The source record connects PCTP, PC to PCTP:PC in cytosol.", "equation": "PCTP + PC → PCTP:PC", "inputs": [ { "node_id": "R-HSA-5215990", "reactome_id": "R-HSA-5215990", "label": "PCTP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-1524163", "reactome_id": "R-ALL-1524163", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8873866", "reactome_id": "R-HSA-8873866", "label": "PCTP:PC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine binding activity of PCTP [cytosol]", "activity_id": "9955874", "ec_numbers": null, "accessions": [ "Q9UKL6" ] } ], "citations": [ { "id": "PMID:12055623", "title": "Structure of human phosphatidylcholine transfer protein in complex with its ligand", "journal": "Nat. Struct. Biol.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12055623/" }, { "id": "PMID:20338778", "title": "PC-TP/StARD2: Of membranes and metabolism", "journal": "Trends Endocrinol. Metab.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20338778/" }, { "id": "PMID:23901139", "title": "Phosphatidylcholine transfer protein interacts with thioesterase superfamily member 2 to attenuate insulin signaling", "journal": "Sci Signal", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23901139/" }, { "id": "PMID:24732803", "title": "Thioesterase superfamily member 2 (Them2) and phosphatidylcholine transfer protein (PC-TP) interact to promote fatty acid oxidation and control glucose utilization", "journal": "Mol. Cell. Biol.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24732803/" } ], "x": 2660, "y": 1871.376712328767, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8873834", "label": "STARD10 transports PC from ER membrane to lamellar body membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane · lamellar body membrane", "compartment_ids": [ "GO:0005829", "GO:0005789", "GO:0097232" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8873834", "summary": "STARD10 transports PC from ER membrane to lamellar body membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects STARD10:LPCAT1:PC to STARD10:PC, LPCAT1 in cytosol · endoplasmic reticulum membrane · lamellar body membrane.", "equation": "STARD10:LPCAT1:PC → STARD10:PC + LPCAT1", "inputs": [ { "node_id": "R-HSA-8873921", "reactome_id": "R-HSA-8873921", "label": "STARD10:LPCAT1:PC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8873863", "reactome_id": "R-HSA-8873863", "label": "STARD10:PC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lamellar body membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1498772", "reactome_id": "R-HSA-1498772", "label": "LPCAT1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "lipid carrier activity of STARD10:LPCAT1:PC [endoplasmic reticulum membrane]", "activity_id": "9955875", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15911624", "title": "StarD10, a START domain protein overexpressed in breast cancer, functions as a phospholipid transfer protein", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15911624/" }, { "id": "PMID:17561512", "title": "Phosphorylation of StarD10 on serine 284 by casein kinase II modulates its lipid transfer activity", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17561512/" }, { "id": "PMID:26048993", "title": "Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) Specifically Interacts with Phospholipid Transfer Protein StarD10 to Facilitate Surfactant Phospholipid Trafficking in Alveolar Type II Cells", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26048993/" } ], "x": 2381.3333333333335, "y": 1892.6917808219177, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8873850", "label": "STARD5 binds DCA, LCA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8873850", "summary": "STARD5 binds DCA, LCA is a Reactome release 97 curated reaction event. The source record connects STARD5, DCA, LCA to STARD5:DCA/LCA in cytosol.", "equation": "STARD5 + DCA, LCA → STARD5:DCA/LCA", "inputs": [ { "node_id": "R-HSA-196114", "reactome_id": "R-HSA-196114", "label": "STARD5", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-8873845", "reactome_id": "R-ALL-8873845", "label": "DCA, LCA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8873894", "reactome_id": "R-HSA-8873894", "label": "STARD5:DCA/LCA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "bile acid binding activity of STARD5 [cytosol]", "activity_id": "9957261", "ec_numbers": null, "accessions": [ "Q9NSY2" ] } ], "citations": [ { "id": "PMID:23337244", "title": "STARD5 specific ligand binding: comparison with STARD1 and STARD4 subfamilies", "journal": "Mol. Cell. Endocrinol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23337244/" }, { "id": "PMID:23018617", "title": "StAR-related lipid transfer domain protein 5 binds primary bile acids", "journal": "J. Lipid Res.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23018617/" } ], "x": 5008.666666666667, "y": 1812.9621212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8873923", "label": "STARD10 binds LPCAT1 and PC", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8873923", "summary": "STARD10 binds LPCAT1 and PC is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects STARD10, LPCAT1, PC to STARD10:LPCAT1:PC in cytosol · endoplasmic reticulum membrane.", "equation": "STARD10 + LPCAT1 + PC → STARD10:LPCAT1:PC", "inputs": [ { "node_id": "R-HSA-8873867", "reactome_id": "R-HSA-8873867", "label": "STARD10", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-1498772", "reactome_id": "R-HSA-1498772", "label": "LPCAT1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8873921", "reactome_id": "R-HSA-8873921", "label": "STARD10:LPCAT1:PC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine binding activity of STARD10 [cytosol]", "activity_id": "9956306", "ec_numbers": null, "accessions": [ "Q9Y365" ] } ], "citations": [ { "id": "PMID:15911624", "title": "StarD10, a START domain protein overexpressed in breast cancer, functions as a phospholipid transfer protein", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15911624/" }, { "id": "PMID:17561512", "title": "Phosphorylation of StarD10 on serine 284 by casein kinase II modulates its lipid transfer activity", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17561512/" }, { "id": "PMID:26048993", "title": "Lysophosphatidylcholine Acyltransferase 1 (LPCAT1) Specifically Interacts with Phospholipid Transfer Protein StarD10 to Facilitate Surfactant Phospholipid Trafficking in Alveolar Type II Cells", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26048993/" } ], "x": 2938.666666666667, "y": 1871.376712328767, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8873929", "label": "Casein kinase II phosphorylates STARD10", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8873929", "summary": "Casein kinase II phosphorylates STARD10 is a Reactome release 97 curated reaction event. The source record connects STARD10, ATP to p-S284-STARD10, ADP in cytosol.", "equation": "STARD10 + ATP → p-S284-STARD10 + ADP", "inputs": [ { "node_id": "R-HSA-8873867", "reactome_id": "R-HSA-8873867", "label": "STARD10", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-8873930", "reactome_id": "R-HSA-8873930", "label": "p-S284-STARD10", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein serine/threonine kinase activity of Casein kinase II [cytosol]", "activity_id": "8850941", "ec_numbers": [ "2.7.11.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:17561512", "title": "Phosphorylation of StarD10 on serine 284 by casein kinase II modulates its lipid transfer activity", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17561512/" } ], "x": 2381.3333333333335, "y": 1860.7191780821918, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8874435", "label": "THEM86B hydrolyses PMCHO, PMETAM", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8874435", "summary": "THEM86B hydrolyses PMCHO, PMETAM is a Reactome release 97 curated reaction event. The source record connects PMCHO, PMETAM, H2O to fatty aldehyde, GPCHO, GPETAM in endoplasmic reticulum membrane · cytosol.", "equation": "PMCHO, PMETAM + H2O → fatty aldehyde + GPCHO, GPETAM", "inputs": [ { "node_id": "R-ALL-8874447", "reactome_id": "R-ALL-8874447", "label": "PMCHO, PMETAM", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5696116", "reactome_id": "R-ALL-5696116", "label": "fatty aldehyde", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35746", "formula": "CHOR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8874450", "reactome_id": "R-ALL-8874450", "label": "GPCHO, GPETAM", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "ether hydrolase activity of TMEM86B [endoplasmic reticulum membrane]", "activity_id": "8874446", "ec_numbers": [ "3.3.2" ], "accessions": [ "Q8N661" ] } ], "citations": [ { "id": "PMID:21515882", "title": "Purification, identification, and cloning of lysoplasmalogenase, the enzyme that catalyzes hydrolysis of the vinyl ether bond of lysoplasmalogen", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21515882/" }, { "id": "PMID:26463208", "title": "Dysregulation of Plasmalogen Homeostasis Impairs Cholesterol Biosynthesis", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26463208/" } ], "x": 2660, "y": 1860.7191780821918, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8874470", "label": "TNFAIP8 proteins transfer PI(4,5)P2, PI(3,4,5)P3 from cytosolic vesicles to plasma membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytoplasmic vesicle membrane · cytosol · plasma membrane", "compartment_ids": [ "GO:0030659", "GO:0005829", "GO:0005886" ], "rhea_ids": [ "RHEA:85915" ], "source_url": "https://reactome.org/content/detail/R-HSA-8874470", "summary": "TNFAIP8 proteins transfer PI(4,5)P2, PI(3,4,5)P3 from cytosolic vesicles to plasma membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PI(4,5)P2, PI(3,4,5)P3 to PI(4,5)P2, PI(3,4,5)P3 in cytoplasmic vesicle membrane · cytosol · plasma membrane and cross-references RHEA:85915.", "equation": "PI(4,5)P2, PI(3,4,5)P3 → PI(4,5)P2, PI(3,4,5)P3", "inputs": [ { "node_id": "R-ALL-8874484", "reactome_id": "R-ALL-8874484", "label": "PI(4,5)P2, PI(3,4,5)P3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytoplasmic vesicle membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8874460", "reactome_id": "R-ALL-8874460", "label": "PI(4,5)P2, PI(3,4,5)P3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "phosphatidylinositol transfer activity of TNFAIP8 proteins [cytosol]", "activity_id": "8874465", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:21924498", "title": "The TIPE (TNFAIP8) family in inflammation, immunity, and cancer", "journal": "Mol. Immunol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21924498/" }, { "id": "PMID:25479791", "title": "Identical expression profiling of human and murine TIPE3 protein reveals links to its functions", "journal": "J. Histochem. Cytochem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25479791/" }, { "id": "PMID:25242044", "title": "TIPE3 is the transfer protein of lipid second messengers that promote cancer", "journal": "Cancer Cell", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25242044/" }, { "id": "PMID:25314070", "title": "A new TIPE of phosphoinositide regulator in cancer", "journal": "Cancer Cell", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25314070/" } ], "x": 2938.666666666667, "y": 1663.554794520548, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8874745", "label": "ACAA2 tetramer transfers acyl group from Ac-CoA to acyl-CoA forming 3OA-CoA and CoA-SH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:21565" ], "source_url": "https://reactome.org/content/detail/R-HSA-8874745", "summary": "ACAA2 tetramer transfers acyl group from Ac-CoA to acyl-CoA forming 3OA-CoA and CoA-SH is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, acyl-CoA to 3OA-CoA, CoA-SH in mitochondrial matrix and cross-references RHEA:21565.", "equation": "Ac-CoA + acyl-CoA → 3OA-CoA + CoA-SH", "inputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524038", "reactome_id": "R-ALL-1524038", "label": "acyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58342", "formula": "C22H31N7O17P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8874749", "reactome_id": "R-ALL-8874749", "label": "3OA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57347", "formula": "C24H33N7O18P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acetyl-CoA C-acyltransferase activity of ACAA2 tetramer [mitochondrial matrix]", "activity_id": "8874747", "ec_numbers": [ "2.3.1.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:8241273", "title": "Cloning and sequence analysis of a full length cDNA encoding human mitochondrial 3-oxoacyl-CoA thiolase", "journal": "Biochim. Biophys. Acta", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8241273/" }, { "id": "PMID:4721607", "title": "The oxoacyl-coenzyme A thiolases of animal tissues", "journal": "Biochem. J.", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4721607/" } ], "x": 868.6666666666667, "y": 1682.3684210526317, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8875013", "label": "ACSM3,ACSM6 ligate CoA to BUT", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:46173" ], "source_url": "https://reactome.org/content/detail/R-HSA-8875013", "summary": "ACSM3,ACSM6 ligate CoA to BUT is a Reactome release 97 curated reaction event. The source record connects BUT, ATP, CoA-SH to BT-CoA, AMP, PPi in mitochondrial matrix and cross-references RHEA:46173.", "equation": "BUT + ATP + CoA-SH → BT-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-8875042", "reactome_id": "R-ALL-8875042", "label": "BUT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17968", "formula": "C4H7O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77318", "reactome_id": "R-ALL-77318", "label": "BT-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57371", "formula": "C25H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "medium-chain fatty acid-CoA ligase activity of ACSM3,ACSM6 [mitochondrial matrix]", "activity_id": "8875012", "ec_numbers": [ "6.2.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:11470804", "title": "Molecular identification and characterization of two medium-chain acyl-CoA synthetases, MACS1 and the Sa gene product", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11470804/" }, { "id": "PMID:7907320", "title": "Human SA gene locus as a candidate locus for essential hypertension", "journal": "Hypertension", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7907320/" }, { "id": "PMID:11772874", "title": "Association between SAH, an acyl-CoA synthetase gene, and hypertriglyceridemia, obesity, and hypertension", "journal": "Circulation", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11772874/" }, { "id": "PMID:17278971", "title": "SAH gene variants are associated with obesity-related hypertension in Caucasians: the PEGASE Study", "journal": "J. Hypertens.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17278971/" }, { "id": "PMID:18192838", "title": "SAH gene variants revisited in the European Project On Genes in Hypertension", "journal": "J. Hypertens.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18192838/" } ], "x": 311.33333333333337, "y": 1670.298245614035, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8875071", "label": "ACSS3 ligates CoA to CH3COO-", "schema_class": "Reaction", "domain_id": "metabolic-integration", "pathway_ids": [ "ketone-bodies" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:23177" ], "source_url": "https://reactome.org/content/detail/R-HSA-8875071", "summary": "ACSS3 ligates CoA to CH3COO- is a Reactome release 97 curated reaction event. The source record connects CH3COO-, ATP, CoA-SH to Ac-CoA, AMP, PPi in mitochondrial matrix and cross-references RHEA:23177.", "equation": "CH3COO- + ATP + CoA-SH → Ac-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-113539", "reactome_id": "R-ALL-113539", "label": "CH3COO-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acetyl-CoA synthetase activity of ACSS3 [mitochondrial matrix]", "activity_id": "8875070", "ec_numbers": [ "6.2.1.1" ], "accessions": [ "Q9H6R3" ] } ], "citations": [ { "id": "PMID:17762044", "title": "Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome", "journal": "J. Lipid Res.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17762044/" } ], "x": 7740, "y": 311.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8875077", "label": "SLC27A3 ligates CoA-SH to VLCFA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "very-long-chain-acyl-coa" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:54537" ], "source_url": "https://reactome.org/content/detail/R-HSA-8875077", "summary": "SLC27A3 ligates CoA-SH to VLCFA is a Reactome release 97 curated reaction event. The source record connects ATP, CoA-SH, VLCFA to VLCFA-CoA, AMP, PPi in mitochondrial matrix and cross-references RHEA:54537.", "equation": "ATP + CoA-SH + VLCFA → VLCFA-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5696008", "reactome_id": "R-ALL-5696008", "label": "VLCFA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58950", "formula": "CO2R", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5696003", "reactome_id": "R-ALL-5696003", "label": "VLCFA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:138261", "formula": "C22H31N7O17P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159448", "reactome_id": "R-ALL-159448", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-159450", "reactome_id": "R-ALL-159450", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "very long-chain fatty acid-CoA ligase activity of SLC27A3 [mitochondrial matrix]", "activity_id": "8875075", "ec_numbers": null, "accessions": [ "Q5K4L6" ] } ], "citations": [ { "id": "PMID:17762044", "title": "Evidence for 26 distinct acyl-coenzyme A synthetase genes in the human genome", "journal": "J. Lipid Res.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17762044/" }, { "id": "PMID:15469937", "title": "Mouse very long-chain Acyl-CoA synthetase 3/fatty acid transport protein 3 catalyzes fatty acid activation but not fatty acid transport in MA-10 cells", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15469937/" } ], "x": 868.6666666666667, "y": 1540.5438596491229, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8875623", "label": "SLC25A18,22 import L-Glu, H+", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport", "malate-aspartate-shuttle" ], "compartment": "cytosol · mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:70955", "RHEA:70957" ], "source_url": "https://reactome.org/content/detail/R-HSA-8875623", "summary": "SLC25A18,22 import L-Glu, H+ is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects L-Glu, H+ to L-Glu, H+ in cytosol · mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:70955, RHEA:70957.", "equation": "L-Glu + H+ → L-Glu + H+", "inputs": [ { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "amino acid:proton symporter activity of SLC25A18,A22 [mitochondrial inner membrane]", "activity_id": "8875618", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11897791", "title": "Identification of the mitochondrial glutamate transporter. Bacterial expression, reconstitution, functional characterization, and tissue distribution of two human isoforms", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11897791/" }, { "id": "PMID:15592994", "title": "Impaired mitochondrial glutamate transport in autosomal recessive neonatal myoclonic epilepsy", "journal": "Am. J. Hum. Genet.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15592994/" }, { "id": "PMID:34679360", "title": "Neonatal Seizures: An Overview of Genetic Causes and Treatment Options", "journal": "Brain Sci", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34679360/" } ], "x": 4730, "y": 780.9, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8875838", "label": "SLC25A19 transports ThDP from cytosol to mitochondrial matrix", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial intermembrane space · mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005758", "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:75471" ], "source_url": "https://reactome.org/content/detail/R-HSA-8875838", "summary": "SLC25A19 transports ThDP from cytosol to mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ThDP to ThDP in mitochondrial intermembrane space · mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:75471.", "equation": "ThDP → ThDP", "inputs": [ { "node_id": "R-ALL-29480", "reactome_id": "R-ALL-29480", "label": "ThDP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58937", "formula": "C12H16N4O7P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8878387", "reactome_id": "R-ALL-8878387", "label": "ThDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58937", "formula": "C12H16N4O7P2S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "thiamine pyrophosphate transmembrane transporter activity of SLC25A19 [mitochondrial inner membrane]", "activity_id": "8875831", "ec_numbers": null, "accessions": [ "Q9HC21" ] } ], "citations": [ { "id": "PMID:18280798", "title": "The evidence that the DNC (SLC25A19) is not the mitochondrial deoxyribonucleotide carrier", "journal": "Mitochondrion", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18280798/" }, { "id": "PMID:20583149", "title": "Amish microcephaly: Long-term survival and biochemical characterization", "journal": "Am. J. Med. Genet. A", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20583149/" } ], "x": 5948.666666666667, "y": 3023.092105263158, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8876789", "label": "CARNMT1 methylates CARN to Anserine", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14206" ], "source_url": "https://reactome.org/content/detail/R-HSA-8876789", "summary": "CARNMT1 methylates CARN to Anserine is a Reactome release 97 curated reaction event. The source record connects CARN, AdoMet to Anserine, AdoHcy, H+ in cytosol and cross-references RHEA:14206.", "equation": "CARN + AdoMet → Anserine + AdoHcy + H+", "inputs": [ { "node_id": "R-ALL-6786250", "reactome_id": "R-ALL-6786250", "label": "CARN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57485", "formula": "C9H14N4O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8876775", "reactome_id": "R-ALL-8876775", "label": "Anserine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58445", "formula": "C10H16N4O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-71285", "reactome_id": "R-ALL-71285", "label": "AdoHcy", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carnosine N-methyltransferase activity of CARNMT1 [cytosol]", "activity_id": "8876777", "ec_numbers": [ "2.1.1.22" ], "accessions": [ "Q8N4J0" ] } ], "citations": [ { "id": "PMID:26001783", "title": "UPF0586 Protein C9orf41 Homolog Is Anserine-producing Methyltransferase", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26001783/" } ], "x": 8018.666666666667, "y": 2030.6875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8876889", "label": "Btn-ACACB:2Mn2+ polymer carboxylates Ac-CoA to form Mal-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:11309" ], "source_url": "https://reactome.org/content/detail/R-HSA-8876889", "summary": "Btn-ACACB:2Mn2+ polymer carboxylates Ac-CoA to form Mal-CoA is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, ATP, HCO3- to ADP, Pi, Mal-CoA in cytosol · mitochondrial outer membrane and cross-references RHEA:11309.", "equation": "Ac-CoA + ATP + HCO3- → ADP + Pi + Mal-CoA + H+", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111627", "reactome_id": "R-ALL-111627", "label": "HCO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17544", "formula": "CHO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29508", "reactome_id": "R-ALL-29508", "label": "Mal-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "acetyl-CoA carboxylase activity of Btn-ACACB:2Mn2+ polymer [mitochondrial outer membrane]", "activity_id": "8876888", "ec_numbers": [ "6.4.1.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:17223360", "title": "Expression, purification, and characterization of human acetyl-CoA carboxylase 2", "journal": "Protein Expr Purif", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17223360/" }, { "id": "PMID:10677481", "title": "The subcellular localization of acetyl-CoA carboxylase 2", "journal": "Proc Natl Acad Sci U S A", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10677481/" }, { "id": "PMID:20457939", "title": "Induced polymerization of mammalian acetyl-CoA carboxylase by MIG12 provides a tertiary level of regulation of fatty acid synthesis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20457939/" }, { "id": "PMID:22123817", "title": "Selectivity in post-translational biotin addition to five human carboxylases", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22123817/" }, { "id": "PMID:18556348", "title": "Hepatic expression of the SPOT 14 (S14) paralog S14-related (Mid1 interacting protein) is regulated by dietary carbohydrate", "journal": "Endocrinology", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18556348/" }, { "id": "PMID:24277613", "title": "Spot14/Mig12 heterocomplex sequesters polymerization and restrains catalytic function of human acetyl-CoA carboxylase 2", "journal": "J. Mol. Recognit.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24277613/" } ], "x": 868.6666666666667, "y": 1534.5087719298247, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8878654", "label": "ACP6 hydrolyses MYS-LPA", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8878654", "summary": "ACP6 hydrolyses MYS-LPA is a Reactome release 97 curated reaction event. The source record connects MYS-LPA, H2O to 1-MMG, Pi in mitochondrial matrix.", "equation": "MYS-LPA + H2O → 1-MMG + Pi", "inputs": [ { "node_id": "R-ALL-8878649", "reactome_id": "R-ALL-8878649", "label": "MYS-LPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62833", "formula": "C17H35O7P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8934414", "reactome_id": "R-ALL-8934414", "label": "1-MMG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:75562", "formula": "C17H34O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113548", "reactome_id": "R-ALL-113548", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lysophosphatidic acid phosphatase activity of ACP6 [mitochondrial matrix]", "activity_id": "8878643", "ec_numbers": [ "3.1.3.106" ], "accessions": [ "Q9NPH0" ] } ], "citations": [ { "id": "PMID:23807634", "title": "Crystal structures and biochemical studies of human lysophosphatidic acid phosphatase type 6", "journal": "Protein Cell", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23807634/" }, { "id": "PMID:10506173", "title": "Isolation of a cDNA encoding human lysophosphatidic acid phosphatase that is involved in the regulation of mitochondrial lipid biosynthesis", "journal": "J. Biol. Chem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10506173/" } ], "x": 2381.3333333333335, "y": 1844.7328767123288, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8878787", "label": "ALPI dimer hydrolyses phosphate monoesters", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:15018" ], "source_url": "https://reactome.org/content/detail/R-HSA-8878787", "summary": "ALPI dimer hydrolyses phosphate monoesters is a Reactome release 97 curated reaction event. The source record connects H2O, phosphate monoester to Pi, Alcohol in extracellular region · plasma membrane and cross-references RHEA:15018.", "equation": "H2O + phosphate monoester → Pi + Alcohol", "inputs": [ { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8878780", "reactome_id": "R-ALL-8878780", "label": "phosphate monoester", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:7794", "formula": "H2O4PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8878776", "reactome_id": "R-ALL-8878776", "label": "Alcohol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30879", "formula": "HOR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alkaline phosphatase activity of ALPI:2Ca2+:Mg2+ dimer [plasma membrane]", "activity_id": "8878778", "ec_numbers": [ "3.1.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:3458202", "title": "Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3458202/" }, { "id": "PMID:24966474", "title": "Alkaline phosphatase: an overview", "journal": "Indian J Clin Biochem", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24966474/" } ], "x": 2381.3333333333335, "y": 1535.664383561644, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8878815", "label": "Formation of 4Fe-4S cluster on ISCA1:ISCA2", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "iron-sulfur" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8878815", "summary": "Formation of 4Fe-4S cluster on ISCA1:ISCA2 is a Reactome release 97 curated reaction event. The source record connects GLRX5:2Fe-2S, ISCA1:ISCA2 to ISCA1:ISCA2:4Fe-4S, GLRX5 in mitochondrial matrix.", "equation": "2 GLRX5:2Fe-2S + ISCA1:ISCA2 → ISCA1:ISCA2:4Fe-4S + 2 GLRX5", "inputs": [ { "node_id": "R-HSA-8878793", "reactome_id": "R-HSA-8878793", "label": "GLRX5:2Fe-2S", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-8878840", "reactome_id": "R-HSA-8878840", "label": "ISCA1:ISCA2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8878796", "reactome_id": "R-HSA-8878796", "label": "ISCA1:ISCA2:4Fe-4S", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-2564761", "reactome_id": "R-HSA-2564761", "label": "GLRX5", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "iron-sulfur transferase activity of ISCA1:ISCA2 [mitochondrial matrix]", "activity_id": "9954150", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25347204", "title": "Formation of [4Fe-4S] clusters in the mitochondrial iron-sulfur cluster assembly machinery", "journal": "J. Am. Chem. Soc.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25347204/" }, { "id": "PMID:22323289", "title": "The human mitochondrial ISCA1, ISCA2, and IBA57 proteins are required for [4Fe-4S] protein maturation", "journal": "Mol. Biol. Cell", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22323289/" }, { "id": "PMID:24733926", "title": "[2Fe-2S] cluster transfer in iron-sulfur protein biogenesis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24733926/" }, { "id": "PMID:20668094", "title": "Characterization of the human HSC20, an unusual DnaJ type III protein, involved in iron-sulfur cluster biogenesis", "journal": "Hum. Mol. Genet.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20668094/" }, { "id": "PMID:21987576", "title": "Specialized function of yeast Isa1 and Isa2 proteins in the maturation of mitochondrial [4Fe-4S] proteins", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21987576/" }, { "id": "PMID:27989128", "title": "[4Fe-4S] Cluster Assembly in Mitochondria and Its Impairment by Copper", "journal": "J. Am. Chem. Soc.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/27989128/" } ], "x": 5670, "y": 3573.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8878914", "label": "B3GALNT1 transfers GalNAc to Gb3Cer to form Gb4Cer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi lumen · Golgi membrane", "compartment_ids": [ "GO:0005796", "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8878914", "summary": "B3GALNT1 transfers GalNAc to Gb3Cer to form Gb4Cer is a Reactome release 97 curated reaction event. The source record connects Gb3Cer, UDP-GalNAc to Gb4Cer, UDP, H+ in Golgi lumen · Golgi membrane.", "equation": "Gb3Cer + UDP-GalNAc → Gb4Cer + UDP + H+", "inputs": [ { "node_id": "R-ALL-8879102", "reactome_id": "R-ALL-8879102", "label": "Gb3Cer", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18313", "formula": "C37H66NO18R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-735700", "reactome_id": "R-ALL-735700", "label": "UDP-GalNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57847", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8879101", "reactome_id": "R-ALL-8879101", "label": "Gb4Cer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:88167", "formula": "C30H50N2O23R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase activity of B3GALNT1 [Golgi membrane]", "activity_id": "8879103", "ec_numbers": [ "2.4.1.79" ], "accessions": [ "O75752" ] } ], "citations": [ { "id": "PMID:10993897", "title": "Expression cloning of human globoside synthase cDNAs. Identification of beta 3Gal-T3 as UDP-N-acetylgalactosamine:globotriaosylceramide beta 1,3-N-acetylgalactosaminyltransferase", "journal": "J. Biol. Chem.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10993897/" }, { "id": "PMID:9582303", "title": "A family of human beta3-galactosyltransferases. Characterization of four members of a UDP-galactose:beta-N-acetyl-glucosamine/beta-nacetyl-galactosamine beta-1,3-galactosyltransferase family", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9582303/" } ], "x": 3878.666666666667, "y": 1720.0192307692307, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-888614", "label": "IDO2 dioxygenates L-Trp to NFK", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24537" ], "source_url": "https://reactome.org/content/detail/R-HSA-888614", "summary": "IDO2 dioxygenates L-Trp to NFK is a Reactome release 97 curated reaction event. The source record connects L-Trp, O2 to NFK in cytosol and cross-references RHEA:24537.", "equation": "L-Trp + O2 → NFK", "inputs": [ { "node_id": "R-ALL-29500", "reactome_id": "R-ALL-29500", "label": "L-Trp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57912", "formula": "C11H12N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-33179", "reactome_id": "R-ALL-33179", "label": "NFK", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58629", "formula": "C11H12N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-tryptophan 2,3-dioxygenase activity of IDO2 [cytosol]", "activity_id": "888607", "ec_numbers": [ "1.13.11.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:17671174", "title": "Novel tryptophan catabolic enzyme IDO2 is the preferred biochemical target of the antitumor indoleamine 2,3-dioxygenase inhibitory compound D-1-methyl-tryptophan", "journal": "Cancer Res", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17671174/" } ], "x": 8018.666666666667, "y": 1859.7083333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8932633", "label": "PON1,2,3:Ca2+ dimers hydrolyse 5-HETEL to 5-HETE", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8932633", "summary": "PON1,2,3:Ca2+ dimers hydrolyse 5-HETEL to 5-HETE is a Reactome release 97 curated reaction event. The source record connects 5-HETEL, H2O to 5-HETE in extracellular region.", "equation": "5-HETEL + H2O → 5-HETE", "inputs": [ { "node_id": "R-ALL-8932801", "reactome_id": "R-ALL-8932801", "label": "5-HETEL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132873", "formula": "C20H38O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8932804", "reactome_id": "R-ALL-8932804", "label": "5-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:65341", "formula": "C20H31O3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "acyl-L-homoserine-lactone lactonohydrolase activity of PON1,2,3:2xCa2+ dimers [extracellular region]", "activity_id": "8932640", "ec_numbers": [ "3.1.1.81" ], "accessions": [] } ], "citations": [ { "id": "PMID:15772423", "title": "Human paraoxonases (PON1, PON2, and PON3) are lactonases with overlapping and distinct substrate specificities", "journal": "J. Lipid Res.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15772423/" }, { "id": "PMID:19082953", "title": "Paraoxonases (PON1, PON2, PON3) analyses in vitro and in vivo in relation to cardiovascular diseases", "journal": "Methods Mol. Biol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/19082953/" } ], "x": 590, "y": 1724.6140350877192, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8932980", "label": "SERINC3,5,(1,2,4) transport L-Ser from cytosol to plasma membrane", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "serine" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [ "RHEA:35031" ], "source_url": "https://reactome.org/content/detail/R-HSA-8932980", "summary": "SERINC3,5,(1,2,4) transport L-Ser from cytosol to plasma membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects L-Ser to L-Ser in cytosol · plasma membrane and cross-references RHEA:35031.", "equation": "L-Ser → L-Ser", "inputs": [ { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8932979", "reactome_id": "R-ALL-8932979", "label": "L-Ser", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of SERINC3,5,(1,2,4) [plasma membrane]", "activity_id": "8932974", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:26703715", "title": "Spotlight on HIV-1 Nef: SERINC3 and SERINC5 Identified as Restriction Factors Antagonized by the Pathogenesis Factor", "journal": "Viruses", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26703715/" }, { "id": "PMID:16120614", "title": "Serinc, an activity-regulated protein family, incorporates serine into membrane lipid synthesis", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16120614/" }, { "id": "PMID:26416733", "title": "SERINC3 and SERINC5 restrict HIV-1 infectivity and are counteracted by Nef", "journal": "Nature", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26416733/" } ], "x": 590, "y": 2994.1111111111113, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8933547", "label": "MCAT transfers Mal from Mal-CoA to NDUFAB1", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8933547", "summary": "MCAT transfers Mal from Mal-CoA to NDUFAB1 is a Reactome release 97 curated reaction event. The source record connects Malonyl-CoA, NDUFAB1 to Mal-PPANT-S112-NDUFAB1, CoA-SH in mitochondrial inner membrane · mitochondrial matrix.", "equation": "Malonyl-CoA + NDUFAB1 → Mal-PPANT-S112-NDUFAB1 + CoA-SH", "inputs": [ { "node_id": "R-ALL-977314", "reactome_id": "R-ALL-977314", "label": "Malonyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-163925", "reactome_id": "R-HSA-163925", "label": "NDUFAB1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8934586", "reactome_id": "R-HSA-8934586", "label": "Mal-PPANT-S112-NDUFAB1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "[acyl-carrier-protein] S-malonyltransferase activity of MCAT [mitochondrial matrix]", "activity_id": "8933543", "ec_numbers": [ "2.3.1.39" ], "accessions": [ "Q8IVS2" ] } ], "citations": [ { "id": "PMID:12882974", "title": "Cloning, expression, characterization, and interaction of two components of a human mitochondrial fatty acid synthase. Malonyltransferase and acyl carrier protein", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12882974/" } ], "x": 590, "y": 1733.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-893583", "label": "kynurenine + 2-oxoglutarate =\u003e 4-(2-aminophenyl)-2,4-dioxobutanoic acid + glutamate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:20965" ], "source_url": "https://reactome.org/content/detail/R-HSA-893583", "summary": "kynurenine + 2-oxoglutarate =\u003e 4-(2-aminophenyl)-2,4-dioxobutanoic acid + glutamate is a Reactome release 97 curated reaction event. The source record connects L-KYN, 2OG to APDBA, L-Glu in mitochondrial matrix and cross-references RHEA:20965.", "equation": "L-KYN + 2OG → APDBA + L-Glu", "inputs": [ { "node_id": "R-ALL-893607", "reactome_id": "R-ALL-893607", "label": "L-KYN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57959", "formula": "C10H12N2O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-893617", "reactome_id": "R-ALL-893617", "label": "APDBA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58147", "formula": "C10H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-kynurenine:2-oxoglutarate transaminase activity of AADAT dimer [mitochondrial matrix]", "activity_id": "893618", "ec_numbers": [ "2.6.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:18056995", "title": "Crystal structure of human kynurenine aminotransferase II", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18056995/" }, { "id": "PMID:13069505", "title": "The transamination of kynurenine", "journal": "J Biol Chem", "year": 1953, "url": "https://pubmed.ncbi.nlm.nih.gov/13069505/" } ], "x": 7740, "y": 1834.7916666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-893593", "label": "phenylalanine + pyruvate =\u003e keto-phenylpyruvate + alanine", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13054" ], "source_url": "https://reactome.org/content/detail/R-HSA-893593", "summary": "phenylalanine + pyruvate =\u003e keto-phenylpyruvate + alanine is a Reactome release 97 curated reaction event. The source record connects L-Phe, PYR to L-Ala, kPPV in cytosol and cross-references RHEA:13054.", "equation": "L-Phe + PYR → L-Ala + kPPV", "inputs": [ { "node_id": "R-ALL-29502", "reactome_id": "R-ALL-29502", "label": "L-Phe", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58095", "formula": "C9H11NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29432", "reactome_id": "R-ALL-29432", "label": "L-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2160487", "reactome_id": "R-ALL-2160487", "label": "kPPV", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18005", "formula": "C9H7O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-phenylalanine:pyruvate transaminase activity of PXLP-KYAT1 dimer [cytosol]", "activity_id": "893585", "ec_numbers": [ "2.6.1.58" ], "accessions": [] } ], "citations": [ { "id": "PMID:8294935", "title": "Purification and characterization of kynurenine aminotransferase I from human brain", "journal": "J Neurochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8294935/" }, { "id": "PMID:19338303", "title": "Structural insight into the inhibition of human kynurenine aminotransferase I/glutamine transaminase K", "journal": "J Med Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19338303/" }, { "id": "PMID:7883047", "title": "Molecular cloning and expression of a cDNA for human kidney cysteine conjugate beta-lyase", "journal": "FEBS Lett", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7883047/" }, { "id": "PMID:15364907", "title": "Crystal structure of human kynurenine aminotransferase I", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15364907/" } ], "x": 7078.666666666667, "y": 1847.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-893596", "label": "PXLP-KYAT1 dimer transaminates L-KYN to KYNA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:65561" ], "source_url": "https://reactome.org/content/detail/R-HSA-893596", "summary": "PXLP-KYAT1 dimer transaminates L-KYN to KYNA is a Reactome release 97 curated reaction event. The source record connects L-KYN, 2OG to KYNA, L-Glu, H2O in cytosol and cross-references RHEA:65561.", "equation": "L-KYN + 2OG → KYNA + L-Glu + H2O", "inputs": [ { "node_id": "R-ALL-29970", "reactome_id": "R-ALL-29970", "label": "L-KYN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57959", "formula": "C10H12N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-893587", "reactome_id": "R-ALL-893587", "label": "KYNA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58454", "formula": "C10H6NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-kynurenine:2-oxoglutarate transaminase activity of PXLP-KYAT1 dimer [cytosol]", "activity_id": "893577", "ec_numbers": [ "2.6.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:8294935", "title": "Purification and characterization of kynurenine aminotransferase I from human brain", "journal": "J Neurochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8294935/" }, { "id": "PMID:19338303", "title": "Structural insight into the inhibition of human kynurenine aminotransferase I/glutamine transaminase K", "journal": "J Med Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19338303/" }, { "id": "PMID:13069505", "title": "The transamination of kynurenine", "journal": "J Biol Chem", "year": 1953, "url": "https://pubmed.ncbi.nlm.nih.gov/13069505/" }, { "id": "PMID:7883047", "title": "Molecular cloning and expression of a cDNA for human kidney cysteine conjugate beta-lyase", "journal": "FEBS Lett", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7883047/" }, { "id": "PMID:15364907", "title": "Crystal structure of human kynurenine aminotransferase I", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15364907/" } ], "x": 8018.666666666667, "y": 1809.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-893597", "label": "4-(2-aminophenyl)-2,4-dioxobutanoate =\u003e kynurenic acid + H2O [mitochondrial]", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:42453" ], "source_url": "https://reactome.org/content/detail/R-HSA-893597", "summary": "4-(2-aminophenyl)-2,4-dioxobutanoate =\u003e kynurenic acid + H2O [mitochondrial] is a Reactome release 97 curated reaction event. The source record connects APDBA to KYNA, H2O in mitochondrial matrix and cross-references RHEA:42453.", "equation": "APDBA → KYNA + H2O", "inputs": [ { "node_id": "R-ALL-893617", "reactome_id": "R-ALL-893617", "label": "APDBA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58147", "formula": "C10H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-893594", "reactome_id": "R-ALL-893594", "label": "KYNA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58454", "formula": "C10H6NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:13069505", "title": "The transamination of kynurenine", "journal": "J Biol Chem", "year": 1953, "url": "https://pubmed.ncbi.nlm.nih.gov/13069505/" } ], "x": 7740, "y": 1884.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-893616", "label": "glutamine + pyruvate =\u003e 2-oxoglutaramate + alanine", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10401" ], "source_url": "https://reactome.org/content/detail/R-HSA-893616", "summary": "glutamine + pyruvate =\u003e 2-oxoglutaramate + alanine is a Reactome release 97 curated reaction event. The source record connects L-Gln, PYR to 2OGA, L-Ala in cytosol and cross-references RHEA:10401.", "equation": "L-Gln + PYR → 2OGA + L-Ala", "inputs": [ { "node_id": "R-ALL-29472", "reactome_id": "R-ALL-29472", "label": "L-Gln", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58359", "formula": "C5H10N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-893598", "reactome_id": "R-ALL-893598", "label": "2OGA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16769", "formula": "C5H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29432", "reactome_id": "R-ALL-29432", "label": "L-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-glutamine:pyruvate transaminase activity of PXLP-KYAT1 dimer [cytosol]", "activity_id": "893579", "ec_numbers": [ "2.6.1.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:8294935", "title": "Purification and characterization of kynurenine aminotransferase I from human brain", "journal": "J Neurochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8294935/" }, { "id": "PMID:19338303", "title": "Structural insight into the inhibition of human kynurenine aminotransferase I/glutamine transaminase K", "journal": "J Med Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19338303/" }, { "id": "PMID:7883047", "title": "Molecular cloning and expression of a cDNA for human kidney cysteine conjugate beta-lyase", "journal": "FEBS Lett", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7883047/" }, { "id": "PMID:15364907", "title": "Crystal structure of human kynurenine aminotransferase I", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15364907/" } ], "x": 8018.666666666667, "y": 1637.65, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8936442", "label": "MARC1,MARC2 reduce N-hydroxylated compounds", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8936442", "summary": "MARC1,MARC2 reduce N-hydroxylated compounds is a Reactome release 97 curated reaction event. The source record connects NOHA, H+, NADH to L-Arg, NAD+, H2O in cytosol · mitochondrial outer membrane.", "equation": "NOHA + H+ + NADH → L-Arg + NAD+ + H2O", "inputs": [ { "node_id": "R-ALL-8936443", "reactome_id": "R-ALL-8936443", "label": "NOHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:47823", "formula": "C6H14N4O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29468", "reactome_id": "R-ALL-29468", "label": "L-Arg", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:32682", "formula": "C6H15N4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity of MARC1:Mo-MPT:MARC2:Mo-MPT:CYB5B:CYB5R3 [mitochondrial outer membrane]", "activity_id": "8936434", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:19053771", "title": "The fourth molybdenum containing enzyme mARC: cloning and involvement in the activation of N-hydroxylated prodrugs", "journal": "J. Med. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/19053771/" }, { "id": "PMID:24423752", "title": "Functional characterization of protein variants encoded by nonsynonymous single nucleotide polymorphisms in MARC1 and MARC2 in healthy Caucasians", "journal": "Drug Metab. Dispos.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24423752/" }, { "id": "PMID:25425164", "title": "The mammalian molybdenum enzymes of mARC", "journal": "J. Biol. Inorg. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25425164/" }, { "id": "PMID:25144769", "title": "The N-reductive system composed of mitochondrial amidoxime reducing component (mARC), cytochrome b5 (CYB5B) and cytochrome b5 reductase (CYB5R) is regulated by fasting and high fat diet in mice", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25144769/" } ], "x": 8018.666666666667, "y": 3058.5196078431372, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8936519", "label": "NQO2:FAD dimer reduces quinones to hydroquinones", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8936519", "summary": "NQO2:FAD dimer reduces quinones to hydroquinones is a Reactome release 97 curated reaction event. The source record connects quinone, RFDHN to hydroquinone, RFNA in cytosol.", "equation": "quinone + RFDHN → hydroquinone + RFNA", "inputs": [ { "node_id": "R-ALL-8936520", "reactome_id": "R-ALL-8936520", "label": "quinone", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36141", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8936516", "reactome_id": "R-ALL-8936516", "label": "RFDHN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:55458", "formula": "C11H16N2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8936507", "reactome_id": "R-ALL-8936507", "label": "hydroquinone", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17594", "formula": "C6H6O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8936514", "reactome_id": "R-ALL-8936514", "label": "RFNA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15927", "formula": "C11H15N2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dihydronicotinamide riboside quinone reductase activity of NQO2:FAD dimer [cytosol]", "activity_id": "8936508", "ec_numbers": [ "1.10.5.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:15350128", "title": "Crystal structure of quinone reductase 2 in complex with resveratrol", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15350128/" }, { "id": "PMID:18254726", "title": "Kinetic, thermodynamic and X-ray structural insights into the interaction of melatonin and analogues with quinone reductase 2", "journal": "Biochem. J.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18254726/" }, { "id": "PMID:9367528", "title": "Catalytic properties of NAD(P)H:quinone oxidoreductase-2 (NQO2), a dihydronicotinamide riboside dependent oxidoreductase", "journal": "Arch. Biochem. Biophys.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9367528/" }, { "id": "PMID:16765324", "title": "NRH:quinone oxidoreductase 2 (NQO2) catalyzes metabolic activation of quinones and anti-tumor drugs", "journal": "Biochem. Pharmacol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16765324/" }, { "id": "PMID:10945627", "title": "Bioactivation of 5-(aziridin-1-yl)-2,4-dinitrobenzamide (CB 1954) by human NAD(P)H quinone oxidoreductase 2: a novel co-substrate-mediated antitumor prodrug therapy", "journal": "Cancer Res.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10945627/" }, { "id": "PMID:8985016", "title": "Cancer chemopreventive activity of resveratrol, a natural product derived from grapes", "journal": "Science", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/8985016/" } ], "x": 7740, "y": 3104.2843137254904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8936849", "label": "AHR:2xHSP90:AIP:PTGES3 binds TCDD", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8936849", "summary": "AHR:2xHSP90:AIP:PTGES3 binds TCDD is a Reactome release 97 curated reaction event. The source record connects AHR:2xHSP90AB1:AIP:PTGES3, TCDD to AHR:TCDD:2xHSP90AB1:AIP:PTGES3 in cytosol.", "equation": "AHR:2xHSP90AB1:AIP:PTGES3 + TCDD → AHR:TCDD:2xHSP90AB1:AIP:PTGES3", "inputs": [ { "node_id": "R-HSA-8936844", "reactome_id": "R-HSA-8936844", "label": "AHR:2xHSP90AB1:AIP:PTGES3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-8936852", "reactome_id": "R-ALL-8936852", "label": "TCDD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28119", "formula": "C12H4Cl4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8936846", "reactome_id": "R-HSA-8936846", "label": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "nuclear receptor activity of AHR:2xHSP90AB1:AIP:PTGES3 [cytosol]", "activity_id": "9958468", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:22759865", "title": "p23 co-chaperone protects the aryl hydrocarbon receptor from degradation in mouse and human cell lines", "journal": "Biochem. Pharmacol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22759865/" }, { "id": "PMID:11274138", "title": "Stable association of hsp90 and p23, but Not hsp70, with active human telomerase", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11274138/" }, { "id": "PMID:7961644", "title": "Dioxin binding activities of polymorphic forms of mouse and human arylhydrocarbon receptors", "journal": "J. Biol. Chem.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7961644/" }, { "id": "PMID:12573486", "title": "Functional role of AhR in the expression of toxic effects by TCDD", "journal": "Biochim. Biophys. Acta", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12573486/" }, { "id": "PMID:18540824", "title": "The aryl hydrocarbon receptor complex and the control of gene expression", "journal": "Crit. Rev. Eukaryot. Gene Expr.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18540824/" }, { "id": "PMID:9447995", "title": "Hepatitis B virus X-associated protein 2 is a subunit of the unliganded aryl hydrocarbon receptor core complex and exhibits transcriptional enhancer activity", "journal": "Mol. Cell. Biol.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9447995/" } ], "x": 7461.333333333333, "y": 3150.049019607843, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8936851", "label": "AHRR binds ARNT", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8936851", "summary": "AHRR binds ARNT is a Reactome release 97 curated reaction event. The source record connects AHRR, ARNT, ARNT2 to AHRR:ARNT in cytosol.", "equation": "AHRR + ARNT, ARNT2 → AHRR:ARNT", "inputs": [ { "node_id": "R-HSA-8936841", "reactome_id": "R-HSA-8936841", "label": "AHRR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-8936837", "reactome_id": "R-HSA-8936837", "label": "ARNT, ARNT2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8936835", "reactome_id": "R-HSA-8936835", "label": "AHRR:ARNT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription repressor activity, RNA polymerase II-specific of AHRR [nucleoplasm]", "activity_id": "9958473", "ec_numbers": null, "accessions": [ "A9YTQ3" ] } ], "citations": [ { "id": "PMID:17980155", "title": "Identification of intracellular localization signals and of mechanisms underlining the nucleocytoplasmic shuttling of human aryl hydrocarbon receptor repressor", "journal": "Biochem. Biophys. Res. Commun.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17980155/" }, { "id": "PMID:18848529", "title": "Regulation of constitutive and inducible AHR signaling: complex interactions involving the AHR repressor", "journal": "Biochem. Pharmacol.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/18848529/" }, { "id": "PMID:18000031", "title": "Repression of aryl hydrocarbon receptor (AHR) signaling by AHR repressor: role of DNA binding and competition for AHR nuclear translocator", "journal": "Mol. Pharmacol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18000031/" } ], "x": 8018.666666666667, "y": 3157.676470588235, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8937169", "label": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3 translocates from cytosol to nucleoplasm", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8937169", "summary": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3 translocates from cytosol to nucleoplasm is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects AHR:TCDD:2xHSP90AB1:AIP:PTGES3 to AHR:TCDD:2xHSP90AB1:AIP:PTGES3 in cytosol · nucleoplasm.", "equation": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3 → AHR:TCDD:2xHSP90AB1:AIP:PTGES3", "inputs": [ { "node_id": "R-HSA-8936846", "reactome_id": "R-HSA-8936846", "label": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8937154", "reactome_id": "R-HSA-8937154", "label": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "nuclear receptor activity of AHR:TCDD:2xHSP90AB1:AIP:PTGES3 [cytosol]", "activity_id": "9958474", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:18540824", "title": "The aryl hydrocarbon receptor complex and the control of gene expression", "journal": "Crit. Rev. Eukaryot. Gene Expr.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18540824/" }, { "id": "PMID:11259606", "title": "The hsp90 chaperone complex regulates intracellular localization of the dioxin receptor", "journal": "Mol. Cell. Biol.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11259606/" } ], "x": 7461.333333333333, "y": 3165.3039215686276, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8937177", "label": "AHR:TCDD binds ARNT", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8937177", "summary": "AHR:TCDD binds ARNT is a Reactome release 97 curated reaction event. The source record connects AHR:TCDD, ARNT, ARNT2 to AHR:TCDD:ARNT in nucleoplasm.", "equation": "AHR:TCDD + ARNT, ARNT2 → AHR:TCDD:ARNT", "inputs": [ { "node_id": "R-HSA-8937150", "reactome_id": "R-HSA-8937150", "label": "AHR:TCDD", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-8936837", "reactome_id": "R-HSA-8936837", "label": "ARNT, ARNT2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8937203", "reactome_id": "R-HSA-8937203", "label": "AHR:TCDD:ARNT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription activator activity, RNA polymerase II-specific of ARNT, ARNT2 [nucleoplasm]", "activity_id": "9958470", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10409767", "title": "Multiple roles of ligand in transforming the dioxin receptor to an active basic helix-loop-helix/PAS transcription factor complex with the nuclear protein Arnt", "journal": "Mol. Cell. Biol.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10409767/" } ], "x": 7740, "y": 3165.3039215686276, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8937191", "label": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3 dissociates", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8937191", "summary": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3 dissociates is a Reactome release 97 curated reaction event. The source record connects AHR:TCDD:2xHSP90AB1:AIP:PTGES3 to AHR:TCDD, 2xHSP90AB1:AIP:PTGES3 in nucleoplasm.", "equation": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3 → AHR:TCDD + 2xHSP90AB1:AIP:PTGES3", "inputs": [ { "node_id": "R-HSA-8937154", "reactome_id": "R-HSA-8937154", "label": "AHR:TCDD:2xHSP90AB1:AIP:PTGES3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8937150", "reactome_id": "R-HSA-8937150", "label": "AHR:TCDD", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-8937201", "reactome_id": "R-HSA-8937201", "label": "2xHSP90AB1:AIP:PTGES3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "nuclear receptor activity of AHR:TCDD:2xHSP90AB1:AIP:PTGES3 [nucleoplasm]", "activity_id": "9958471", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10409767", "title": "Multiple roles of ligand in transforming the dioxin receptor to an active basic helix-loop-helix/PAS transcription factor complex with the nuclear protein Arnt", "journal": "Mol. Cell. Biol.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10409767/" } ], "x": 8018.666666666667, "y": 3165.3039215686276, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8937419", "label": "CBR3 reduces DOX to DOXOL", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8937419", "summary": "CBR3 reduces DOX to DOXOL is a Reactome release 97 curated reaction event. The source record connects NADPH, doxorubicin to DOXOL, NADP+ in cytosol.", "equation": "NADPH + doxorubicin → DOXOL + NADP+", "inputs": [ { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9659674", "reactome_id": "R-ALL-9659674", "label": "doxorubicin", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8937418", "reactome_id": "R-ALL-8937418", "label": "DOXOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133817", "formula": "C27H31NO11", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "carbonyl reductase (NADPH) activity of CBR3 [cytosol]", "activity_id": "8937403", "ec_numbers": [ "1.1.1.184" ], "accessions": [ "O75828" ] } ], "citations": [ { "id": "PMID:25446851", "title": "Metabolism of doxorubicin to the cardiotoxic metabolite doxorubicinol is increased in a mouse model of chronic glutathione deficiency: A potential role for carbonyl reductase 3", "journal": "Chem. Biol. Interact.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25446851/" }, { "id": "PMID:2897122", "title": "Doxorubicin cardiotoxicity may be caused by its metabolite, doxorubicinol", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2897122/" }, { "id": "PMID:20007405", "title": "Naturally occurring variants of human CBR3 alter anthracycline in vitro metabolism", "journal": "J. Pharmacol. Exp. Ther.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20007405/" } ], "x": 7740, "y": 3119.5392156862745, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8937442", "label": "CES3 hydrolyses CHEST to CHOL and LCFA(-)", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · lipid droplet · transport vesicle membrane", "compartment_ids": [ "GO:0005829", "GO:0005811", "GO:0030658" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8937442", "summary": "CES3 hydrolyses CHEST to CHOL and LCFA(-) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H2O, CHEST to CHOL, LCFA(-) in cytosol · lipid droplet · transport vesicle membrane.", "equation": "H2O + CHEST → CHOL + LCFA(-)", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-163579", "reactome_id": "R-ALL-163579", "label": "CHEST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17002", "formula": "C28H45O2R", "compartment": "lipid droplet", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-216762", "reactome_id": "R-ALL-216762", "label": "CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "transport vesicle membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8876730", "reactome_id": "R-ALL-8876730", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carboxylic ester hydrolase activity of CES3 [cytosol]", "activity_id": "8937438", "ec_numbers": [ "3.1.1.1" ], "accessions": [ "Q6UWW8" ] } ], "citations": [ { "id": "PMID:22700792", "title": "Identification of a novel intracellular cholesteryl ester hydrolase (carboxylesterase 3) in human macrophages: compensatory increase in its expression after carboxylesterase 1 silencing", "journal": "Am. J. Physiol., Cell Physiol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22700792/" }, { "id": "PMID:20422440", "title": "Mammalian carboxylesterase 3: comparative genomics and proteomics", "journal": "Genetica", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20422440/" } ], "x": 8018.666666666667, "y": 3104.2843137254904, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8938073", "label": "PARPs transfer ADP-D-ribose to proteins (poly(ADP-ribosyl)ation)", "schema_class": "Polymerisation", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:58225" ], "source_url": "https://reactome.org/content/detail/R-HSA-8938073", "summary": "PARPs transfer ADP-D-ribose to proteins (poly(ADP-ribosyl)ation) is a Reactome release 97 curated reaction event. The source record connects NAD+, acceptor protein to NAM, ADP-D-ribosyl-acceptor protein in cytosol and cross-references RHEA:58225.", "equation": "3 NAD+ + acceptor protein → 3 NAM + ADP-D-ribosyl-acceptor protein", "inputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9938200", "reactome_id": "R-HSA-9938200", "label": "acceptor protein", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "GenomeEncodedEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-197277", "reactome_id": "R-ALL-197277", "label": "NAM", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9938174", "reactome_id": "R-HSA-9938174", "label": "ADP-D-ribosyl-acceptor protein", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "GenomeEncodedEntity", "currency": false } ], "catalysts": [ { "label": "NAD+ poly-ADP-ribosyltransferase activity of PARPs [cytosol]", "activity_id": "8938084", "ec_numbers": [ "2.4.2.30" ], "accessions": [] } ], "citations": [ { "id": "PMID:22701565", "title": "PARP16/ARTD15 is a novel endoplasmic-reticulum-associated mono-ADP-ribosyltransferase that interacts with, and modifies karyopherin-ß1", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22701565/" }, { "id": "PMID:15674325", "title": "PARP-10, a novel Myc-interacting protein with poly(ADP-ribose) polymerase activity, inhibits transformation", "journal": "Oncogene", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15674325/" }, { "id": "PMID:23230272", "title": "BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8", "journal": "Mol. Cell. Biol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23230272/" } ], "x": 5670, "y": 3043.565789473684, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8938076", "label": "CD38 hydrolyses NAD+ to NAM and ADP-ribose", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:16302" ], "source_url": "https://reactome.org/content/detail/R-HSA-8938076", "summary": "CD38 hydrolyses NAD+ to NAM and ADP-ribose is a Reactome release 97 curated reaction event. The source record connects NAD+, H2O to NAM, ADP-ribose, H+ in extracellular region · plasma membrane and cross-references RHEA:16302.", "equation": "NAD+ + H2O → NAM + ADP-ribose + H+", "inputs": [ { "node_id": "R-ALL-352330", "reactome_id": "R-ALL-352330", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8870343", "reactome_id": "R-ALL-8870343", "label": "NAM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8870342", "reactome_id": "R-ALL-8870342", "label": "ADP-ribose", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57967", "formula": "C15H21N5O14P2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NAD+ nucleosidase activity, cyclic ADP-ribose generating of CD38 [plasma membrane]", "activity_id": "8938077", "ec_numbers": [ "3.2.2.6" ], "accessions": [ "P28907" ] } ], "citations": [ { "id": "PMID:16690024", "title": "NAADP+ synthesis from cADPRP and nicotinic acid by ADP-ribosyl cyclases", "journal": "Biochem. Biophys. Res. Commun.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16690024/" }, { "id": "PMID:8527488", "title": "Cyclic ADP-ribose and its metabolic enzymes", "journal": "Biochimie", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8527488/" }, { "id": "PMID:10331643", "title": "Structures and activities of cyclic ADP-ribose, NAADP and their metabolic enzymes", "journal": "Mol. Cell. Biochem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10331643/" }, { "id": "PMID:22822066", "title": "Cyclic ADP-ribose and nicotinic acid adenine dinucleotide phosphate (NAADP) as messengers for calcium mobilization", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22822066/" } ], "x": 5948.666666666667, "y": 2849.065789473684, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8938300", "label": "Vanin hydrolyses pantetheine to PanK, 2AET", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [ "RHEA:13446" ], "source_url": "https://reactome.org/content/detail/R-HSA-8938300", "summary": "Vanin hydrolyses pantetheine to PanK, 2AET is a Reactome release 97 curated reaction event. The source record connects Pantetheine to 2AET, PanK in cytosol · plasma membrane and cross-references RHEA:13446.", "equation": "Pantetheine → 2AET + PanK", "inputs": [ { "node_id": "R-ALL-8939157", "reactome_id": "R-ALL-8939157", "label": "Pantetheine", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16753", "formula": "C11H22N2O4S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9837424", "reactome_id": "R-ALL-9837424", "label": "2AET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58029", "formula": "C2H8NS", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-199192", "reactome_id": "R-ALL-199192", "label": "PanK", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29032", "formula": "C9H16NO5", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pantetheine hydrolase activity of VNN1,VNN2 [plasma membrane]", "activity_id": "8939020", "ec_numbers": [ "3.5.1.92" ], "accessions": [] } ], "citations": [ { "id": "PMID:10567687", "title": "Is pantetheinase the actual identity of mouse and human vanin-1 proteins?", "journal": "FEBS Lett.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10567687/" }, { "id": "PMID:32234503", "title": "Regulation of coenzyme A levels by degradation: the 'Ins and Outs'", "journal": "Prog Lipid Res", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32234503/" }, { "id": "PMID:8934567", "title": "Vanin-1, a novel GPI-linked perivascular molecule involved in thymus homing", "journal": "Immunity", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8934567/" } ], "x": 5670, "y": 3012.8552631578946, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8938314", "label": "ENPPs hydrolyse CoA-SH to PPANT, PAP", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:64989" ], "source_url": "https://reactome.org/content/detail/R-HSA-8938314", "summary": "ENPPs hydrolyse CoA-SH to PPANT, PAP is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CoA-SH, H2O to PPANT, PAP in extracellular region · plasma membrane and cross-references RHEA:64989.", "equation": "CoA-SH + H2O → PPANT + PAP", "inputs": [ { "node_id": "R-ALL-8939024", "reactome_id": "R-ALL-8939024", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8939005", "reactome_id": "R-ALL-8939005", "label": "PPANT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61723", "formula": "C11H21N2O7PS", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8942170", "reactome_id": "R-ALL-8942170", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity of 2xENPP1, 2xENPP2, 2xENPP3 [plasma membrane]", "activity_id": "8939004", "ec_numbers": [ "3" ], "accessions": [] } ], "citations": [ { "id": "PMID:12757929", "title": "Physiological and pathophysiological functions of the ecto-nucleotide pyrophosphatase/phosphodiesterase family", "journal": "Biochim. Biophys. Acta", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12757929/" } ], "x": 5948.666666666667, "y": 3033.328947368421, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-8939039", "label": "PPANT translocates from extracellular region to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8939039", "summary": "PPANT translocates from extracellular region to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PPANT to PPANT in cytosol · extracellular region.", "equation": "PPANT → PPANT", "inputs": [ { "node_id": "R-ALL-8939005", "reactome_id": "R-ALL-8939005", "label": "PPANT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61723", "formula": "C11H21N2O7PS", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-196767", "reactome_id": "R-ALL-196767", "label": "PPANT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61723", "formula": "C11H21N2O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:26322826", "title": "Extracellular 4'-phosphopantetheine is a source for intracellular coenzyme A synthesis", "journal": "Nat. Chem. Biol.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26322826/" } ], "x": 5948.666666666667, "y": 3145.934210526316, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8939959", "label": "NMNAT2 transfers an adenylyl group from ATP to NMN to yield NAD+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · Golgi membrane", "compartment_ids": [ "GO:0005829", "GO:0000139" ], "rhea_ids": [ "RHEA:21361" ], "source_url": "https://reactome.org/content/detail/R-HSA-8939959", "summary": "NMNAT2 transfers an adenylyl group from ATP to NMN to yield NAD+ is a Reactome release 97 curated reaction event. The source record connects NMN, ATP, H+ to NAD+, PPi in cytosol · Golgi membrane and cross-references RHEA:21361.", "equation": "NMN + ATP + H+ → NAD+ + PPi", "inputs": [ { "node_id": "R-ALL-8869353", "reactome_id": "R-ALL-8869353", "label": "NMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:14649", "formula": "C11H14N2O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "nicotinamide-nucleotide adenylyltransferase activity of NMNAT2:Mg2+ [Golgi membrane]", "activity_id": "8939960", "ec_numbers": [ "2.7.7.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16118205", "title": "Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16118205/" }, { "id": "PMID:20388704", "title": "Isoform-specific targeting and interaction domains in human nicotinamide mononucleotide adenylyltransferases", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20388704/" }, { "id": "PMID:12359228", "title": "Identification of a novel human nicotinamide mononucleotide adenylyltransferase", "journal": "Biochem Biophys Res Commun", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12359228/" }, { "id": "PMID:17402747", "title": "Initial-rate kinetics of human NMN-adenylyltransferases: substrate and metal ion specificity, inhibition by products and multisubstrate analogues, and isozyme contributions to NAD+ biosynthesis", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17402747/" }, { "id": "PMID:14516279", "title": "Characterization of human brain nicotinamide 5'-mononucleotide adenylyltransferase-2 and expression in human pancreas", "journal": "Biochem J", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/14516279/" } ], "x": 5391.333333333333, "y": 2859.3026315789475, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8940070", "label": "NT5E:Zn2+ hydrolyses NAD+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8940070", "summary": "NT5E:Zn2+ hydrolyses NAD+ is a Reactome release 97 curated reaction event. The source record connects H2O, NAD+ to NMN, Pi, AMP in extracellular region · plasma membrane.", "equation": "H2O + NAD+ → NMN + Pi + AMP + 2 H+", "inputs": [ { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-352330", "reactome_id": "R-ALL-352330", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8940067", "reactome_id": "R-ALL-8940067", "label": "NMN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:14649", "formula": "C11H14N2O8P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109275", "reactome_id": "R-ALL-109275", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5E:Zn2+ dimer [plasma membrane]", "activity_id": "109273", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:21933152", "title": "The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/21933152/" }, { "id": "PMID:2129526", "title": "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2129526/" }, { "id": "PMID:1637327", "title": "5'-nucleotidase: molecular structure and functional aspects", "journal": "Biochem J", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1637327/" } ], "x": 5948.666666666667, "y": 3053.8026315789475, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8940074", "label": "NT5E:Zn2+ hydrolyses NMN", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [ "RHEA:30816" ], "source_url": "https://reactome.org/content/detail/R-HSA-8940074", "summary": "NT5E:Zn2+ hydrolyses NMN is a Reactome release 97 curated reaction event. The source record connects H2O, NMN to NR, Pi in extracellular region · plasma membrane and cross-references RHEA:30816.", "equation": "H2O + NMN → NR + Pi", "inputs": [ { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-8940067", "reactome_id": "R-ALL-8940067", "label": "NMN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:14649", "formula": "C11H14N2O8P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8940077", "reactome_id": "R-ALL-8940077", "label": "NR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15927", "formula": "C11H15N2O5", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109277", "reactome_id": "R-ALL-109277", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5'-nucleotidase activity of NT5E:Zn2+ dimer [plasma membrane]", "activity_id": "109273", "ec_numbers": [ "3.1.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:21933152", "title": "The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism", "journal": "Biochem. J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/21933152/" }, { "id": "PMID:2129526", "title": "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2129526/" }, { "id": "PMID:1637327", "title": "5'-nucleotidase: molecular structure and functional aspects", "journal": "Biochem J", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1637327/" } ], "x": 5670, "y": 2869.5394736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8940134", "label": "FOLR2 binds FOLA", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "extracellular region · plasma membrane", "compartment_ids": [ "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8940134", "summary": "FOLR2 binds FOLA is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects FOLA, FOLR2 to FOLR2:FOLA in extracellular region · plasma membrane.", "equation": "FOLA + FOLR2 → FOLR2:FOLA", "inputs": [ { "node_id": "R-ALL-200716", "reactome_id": "R-ALL-200716", "label": "FOLA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62501", "formula": "C19H17N7O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-8940133", "reactome_id": "R-HSA-8940133", "label": "FOLR2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8940118", "reactome_id": "R-HSA-8940118", "label": "FOLR2:FOLA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "folic acid receptor activity of FOLR2 [plasma membrane]", "activity_id": "9954163", "ec_numbers": null, "accessions": [ "P14207" ] } ], "citations": [ { "id": "PMID:2605182", "title": "Homologous membrane folate binding proteins in human placenta: cloning and sequence of a cDNA", "journal": "Biochemistry", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2605182/" }, { "id": "PMID:23934049", "title": "Structures of human folate receptors reveal biological trafficking states and diversity in folate and antifolate recognition", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23934049/" }, { "id": "PMID:4066659", "title": "Studies of the role of a particulate folate-binding protein in the uptake of 5-methyltetrahydrofolate by cultured human KB cells", "journal": "J. Biol. Chem.", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/4066659/" } ], "x": 4730, "y": 3138.9545454545455, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8941701", "label": "UGT1A10 transfers GlcA from UDP-GlcA to GCTN", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8941701", "summary": "UGT1A10 transfers GlcA from UDP-GlcA to GCTN is a Reactome release 97 curated reaction event. The source record connects GCTN, UDP-GlcA to GCTN4OG, UDP in endoplasmic reticulum membrane · endoplasmic reticulum lumen.", "equation": "GCTN + UDP-GlcA → GCTN4OG + UDP", "inputs": [ { "node_id": "R-ALL-8941723", "reactome_id": "R-ALL-8941723", "label": "GCTN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:34778", "formula": "C16H12O5", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174384", "reactome_id": "R-ALL-174384", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8941725", "reactome_id": "R-ALL-8941725", "label": "GCTN4OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747564", "formula": "C22H19O11", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158602", "reactome_id": "R-ALL-158602", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucuronosyltransferase activity of UGT1A10 [endoplasmic reticulum membrane]", "activity_id": "8941722", "ec_numbers": [ "2.4.1.17" ], "accessions": [ "Q9HAW8" ] } ], "citations": [ { "id": "PMID:19545173", "title": "Structure and concentration changes affect characterization of UGT isoform-specific metabolism of isoflavones", "journal": "Mol. Pharm.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19545173/" }, { "id": "PMID:27556486", "title": "Natural Polyphenols for Prevention and Treatment of Cancer", "journal": "Nutrients", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27556486/" }, { "id": "PMID:18052085", "title": "Commentary: bioavailability of flavonoids and polyphenols: call to arms", "journal": "Mol. Pharm.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/18052085/" } ], "x": 7461.333333333333, "y": 3111.9117647058824, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8942208", "label": "ALOXE3 isomerises 12R-HpETE to HXA3", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8942208", "summary": "ALOXE3 isomerises 12R-HpETE to HXA3 is a Reactome release 97 curated reaction event. The source record connects 12R-HpETE to HXA3 in cytosol.", "equation": "12R-HpETE → HXA3", "inputs": [ { "node_id": "R-ALL-2142790", "reactome_id": "R-ALL-2142790", "label": "12R-HpETE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:75230", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8942209", "reactome_id": "R-ALL-8942209", "label": "HXA3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132127", "formula": "C20H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydroperoxy icosatetraenoate isomerase activity of ALOXE3 [cytosol]", "activity_id": "8942207", "ec_numbers": [ "5.4.4.7" ], "accessions": [ "Q9BYJ1" ] } ], "citations": [ { "id": "PMID:12881489", "title": "The lipoxygenase gene ALOXE3 implicated in skin differentiation encodes a hydroperoxide isomerase", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12881489/" }, { "id": "PMID:21558561", "title": "Lipoxygenases mediate the effect of essential fatty acid in skin barrier formation: a proposed role in releasing omega-hydroxyceramide for construction of the corneocyte lipid envelope", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21558561/" }, { "id": "PMID:15629692", "title": "Mutations associated with a congenital form of ichthyosis (NCIE) inactivate the epidermal lipoxygenases 12R-LOX and eLOX3", "journal": "Biochim. Biophys. Acta", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15629692/" }, { "id": "PMID:26370990", "title": "Homozygous ALOXE3 Nonsense Variant Identified in a Patient with Non-Bullous Congenital Ichthyosiform Erythroderma Complicated by Superimposed Bullous Majocchi's Granuloma: The Consequences of Skin Barrier Dysfunction", "journal": "Int J Mol Sci", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26370990/" } ], "x": 868.6666666666667, "y": 1733.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8942344", "label": "FOLR2 transports FOLA from extracellular region to cytosol", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "extracellular region · plasma membrane · cytosol", "compartment_ids": [ "GO:0005576", "GO:0005886", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8942344", "summary": "FOLR2 transports FOLA from extracellular region to cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects FOLR2:FOLA to FOLR2, FOLA in extracellular region · plasma membrane · cytosol.", "equation": "FOLR2:FOLA → FOLR2 + FOLA", "inputs": [ { "node_id": "R-HSA-8940118", "reactome_id": "R-HSA-8940118", "label": "FOLR2:FOLA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8940133", "reactome_id": "R-HSA-8940133", "label": "FOLR2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-197965", "reactome_id": "R-ALL-197965", "label": "FOLA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62501", "formula": "C19H17N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:4066659", "title": "Studies of the role of a particulate folate-binding protein in the uptake of 5-methyltetrahydrofolate by cultured human KB cells", "journal": "J. Biol. Chem.", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/4066659/" }, { "id": "PMID:23934049", "title": "Structures of human folate receptors reveal biological trafficking states and diversity in folate and antifolate recognition", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23934049/" } ], "x": 4451.333333333333, "y": 3174.318181818182, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-8942575", "label": "N-acetylaspartylglutamate synthase A ligates NAA, L-Glu forming NAAG", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "glutamate-glutamine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:40036" ], "source_url": "https://reactome.org/content/detail/R-HSA-8942575", "summary": "N-acetylaspartylglutamate synthase A ligates NAA, L-Glu forming NAAG is a Reactome release 97 curated reaction event. The source record connects L-Glu, ATP, NAASP to ADP, NAAG, H+ in cytosol and cross-references RHEA:40036.", "equation": "L-Glu + ATP + NAASP → ADP + NAAG + H+ + Pi", "inputs": [ { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5693772", "reactome_id": "R-ALL-5693772", "label": "NAASP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16953", "formula": "C6H7NO5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5693762", "reactome_id": "R-ALL-5693762", "label": "NAAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76931", "formula": "C11H13N2O8", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N-acetyl-L-aspartate-L-glutamate ligase activity of RIMKLA, RIMKLB [cytosol]", "activity_id": "8942574", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:20657015", "title": "Molecular identification of N-acetylaspartylglutamate synthase and beta-citrylglutamate synthase", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20657015/" } ], "x": 8018.666666666667, "y": 1547.95, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8943279", "label": "GGT dimers hydrolyse GSH", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "biological-oxidations", "glutathione", "phase-two" ], "compartment": "plasma membrane · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:28808" ], "source_url": "https://reactome.org/content/detail/R-HSA-8943279", "summary": "GGT dimers hydrolyse GSH is a Reactome release 97 curated reaction event. The source record connects GSH, H2O to CysGly, L-Glu in plasma membrane · extracellular region and cross-references RHEA:28808.", "equation": "GSH + H2O → CysGly + L-Glu", "inputs": [ { "node_id": "R-ALL-1247892", "reactome_id": "R-ALL-1247892", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16856", "formula": "C10H17N3O6S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1247934", "reactome_id": "R-ALL-1247934", "label": "CysGly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61694", "formula": "C5H10N2O3S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-210382", "reactome_id": "R-ALL-210382", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione gamma-glutamate hydrolase activity of GGT dimers [plasma membrane]", "activity_id": "8943271", "ec_numbers": [ "3.4.19.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:17924658", "title": "Kinetic characterization and identification of the acylation and glycosylation sites of recombinant human gamma-glutamyltranspeptidase", "journal": "Biochemistry", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17924658/" }, { "id": "PMID:8813074", "title": "Immunohistochemical detection of gamma-glutamyl transpeptidase in normal human tissue", "journal": "J. Histochem. Cytochem.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8813074/" }, { "id": "PMID:18357469", "title": "The human gamma-glutamyltransferase gene family", "journal": "Hum Genet", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18357469/" }, { "id": "PMID:2573352", "title": "Different gamma-glutamyl transpeptidase mRNAs are expressed in human liver and kidney", "journal": "Biochem Biophys Res Commun", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2573352/" }, { "id": "PMID:19463", "title": "Human kidney gamma-glutamyl transpeptidase. Catalytic properties, subunit structure, and localization of the gamma-glutamyl binding site on the light subunit", "journal": "J Biol Chem", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/19463/" }, { "id": "PMID:9080", "title": "Hydrolysis and transfer reactions catalyzed by gamma-glutamyl transpeptidase; evidence for separate substrate sites and for high affinity of L-cystine", "journal": "Biochem. Biophys. Res. Commun.", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/9080/" } ], "x": 590, "y": 3556.333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8949399", "label": "CAR translocates from the cytosol to the mitochondrial intermembrane space", "schema_class": "BlackBoxEvent", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "cytosol · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005829", "GO:0005758" ], "rhea_ids": [ "RHEA:34959" ], "source_url": "https://reactome.org/content/detail/R-HSA-8949399", "summary": "CAR translocates from the cytosol to the mitochondrial intermembrane space is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CAR to CAR in cytosol · mitochondrial intermembrane space and cross-references RHEA:34959.", "equation": "CAR → CAR", "inputs": [ { "node_id": "R-ALL-30235", "reactome_id": "R-ALL-30235", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-549207", "reactome_id": "R-ALL-549207", "label": "CAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12440696", "title": "Origami in the outer membrane: the transmembrane arrangement of mitochondrial porins", "journal": "Biochem. Cell Biol.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12440696/" } ], "x": 590, "y": 1640.122807017544, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-8951850", "label": "TSPO:BZRAP1 transports CHOL from outer mitochondrial membrane to inner mitochondrial membrane", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · mitochondrial outer membrane · mitochondrial inner membrane", "compartment_ids": [ "GO:0005829", "GO:0005741", "GO:0005743" ], "rhea_ids": [ "RHEA:39747" ], "source_url": "https://reactome.org/content/detail/R-HSA-8951850", "summary": "TSPO:BZRAP1 transports CHOL from outer mitochondrial membrane to inner mitochondrial membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CHOL to CHOL in cytosol · mitochondrial outer membrane · mitochondrial inner membrane and cross-references RHEA:39747.", "equation": "CHOL → CHOL", "inputs": [ { "node_id": "R-ALL-8951853", "reactome_id": "R-ALL-8951853", "label": "CHOL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-193156", "reactome_id": "R-ALL-193156", "label": "CHOL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16113", "formula": "C27H46O", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cholesterol transfer activity of TSPO:BZRAP1 [mitochondrial outer membrane]", "activity_id": "8951851", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:9915832", "title": "Cloning and characterization of PRAX-1. A new protein that specifically interacts with the peripheral benzodiazepine receptor", "journal": "J. Biol. Chem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9915832/" }, { "id": "PMID:8307574", "title": "The human peripheral benzodiazepine receptor gene: cloning and characterization of alternative splicing in normal tissues and in a patient with congenital lipoid adrenal hyperplasia", "journal": "Genomics", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8307574/" }, { "id": "PMID:2981667", "title": "Peripheral-type benzodiazepine receptors in endocrine organs: autoradiographic localization in rat pituitary, adrenal, and testis", "journal": "Endocrinology", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2981667/" }, { "id": "PMID:3001071", "title": "The peripheral-type benzodiazepine receptor. Localization to the mitochondrial outer membrane", "journal": "J. Biol. Chem.", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3001071/" }, { "id": "PMID:25818881", "title": "Translocator protein-mediated pharmacology of cholesterol transport and steroidogenesis", "journal": "Mol. Cell. Endocrinol.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25818881/" } ], "x": 4730, "y": 1630.25, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8952137", "label": "Phospholipid phosphatase 6 hydrolyses Presqualene diphosphate to presqualene monophosphate", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:67969" ], "source_url": "https://reactome.org/content/detail/R-HSA-8952137", "summary": "Phospholipid phosphatase 6 hydrolyses Presqualene diphosphate to presqualene monophosphate is a Reactome release 97 curated reaction event. The source record connects PSQPP, H2O to PSMP, Pi, H+ in cytosol and cross-references RHEA:67969.", "equation": "PSQPP + H2O → PSMP + Pi + H+", "inputs": [ { "node_id": "R-ALL-191265", "reactome_id": "R-ALL-191265", "label": "PSQPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57310", "formula": "C30H49O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8952135", "reactome_id": "R-ALL-8952135", "label": "PSMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:176803", "formula": "C30H49O4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "hydrolase activity of PLPP6 [plasma membrane]", "activity_id": "8952141", "ec_numbers": [ "3" ], "accessions": [ "Q8IY26" ] } ], "citations": [ { "id": "PMID:16464866", "title": "Identification and functional characterization of a presqualene diphosphate phosphatase", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16464866/" } ], "x": 2660, "y": 2128.7, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8952251", "label": "PLA2G15 hydrolyses LPC to GPCho and LCFA(-)", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8952251", "summary": "PLA2G15 hydrolyses LPC to GPCho and LCFA(-) is a Reactome release 97 curated reaction event. The source record connects lysoPC, H2O to GPCho, LCFA(-) in extracellular region.", "equation": "lysoPC + H2O → GPCho + LCFA(-)", "inputs": [ { "node_id": "R-ALL-2173763", "reactome_id": "R-ALL-2173763", "label": "lysoPC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60479", "formula": "C9H19NO7PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8952253", "reactome_id": "R-ALL-8952253", "label": "GPCho", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16870", "formula": "C8H20NO6P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8954142", "reactome_id": "R-ALL-8954142", "label": "LCFA(-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57560", "formula": "CO2R", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "A2-type glycerophospholipase activity of PLA2G15 [extracellular region]", "activity_id": "8952250", "ec_numbers": [ "3.1.1.4" ], "accessions": [ "Q8NCC3" ] } ], "citations": [ { "id": "PMID:10092508", "title": "Cloning and expression of a novel lysophospholipase which structurally resembles lecithin cholesterol acyltransferase", "journal": "Biochem. Biophys. Res. Commun.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10092508/" }, { "id": "PMID:11790796", "title": "Cloning and characterization of a lysosomal phospholipase A2, 1-O-acylceramide synthase", "journal": "J. Biol. Chem.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11790796/" }, { "id": "PMID:9525960", "title": "Purification and characterization of 1-O-acylceramide synthase, a novel phospholipase A2 with transacylase activity", "journal": "J. Biol. Chem.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9525960/" } ], "x": 2660, "y": 1844.7328767123288, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8952873", "label": "MECR dimer reduces tdec2-CoA to DEC-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "beta-oxidation", "fatty-acid-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:44961" ], "source_url": "https://reactome.org/content/detail/R-HSA-8952873", "summary": "MECR dimer reduces tdec2-CoA to DEC-CoA is a Reactome release 97 curated reaction event. The source record connects tdec2-CoA, NADPH, H+ to DEC-CoA, NADP+ in mitochondrial matrix and cross-references RHEA:44961.", "equation": "tdec2-CoA + NADPH + H+ → DEC-CoA + NADP+", "inputs": [ { "node_id": "R-ALL-77343", "reactome_id": "R-ALL-77343", "label": "tdec2-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61406", "formula": "C31H48N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-77308", "reactome_id": "R-ALL-77308", "label": "DEC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61430", "formula": "C31H50N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "trans-2-enoyl-CoA reductase (NADPH) activity of MECR dimer [mitochondrial matrix]", "activity_id": "8952869", "ec_numbers": [ "1.3.1.38" ], "accessions": [] } ], "citations": [ { "id": "PMID:18479707", "title": "Structural enzymological studies of 2-enoyl thioester reductase of the human mitochondrial FAS II pathway: new insights into its substrate recognition properties", "journal": "J. Mol. Biol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18479707/" }, { "id": "PMID:12654921", "title": "Characterization of 2-enoyl thioester reductase from mammals. An ortholog of YBR026p/MRF1'p of the yeast mitochondrial fatty acid synthesis type II", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12654921/" } ], "x": 311.33333333333337, "y": 1676.3333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8953339", "label": "DNPH1 hydrolyses dGMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:57817" ], "source_url": "https://reactome.org/content/detail/R-HSA-8953339", "summary": "DNPH1 hydrolyses dGMP is a Reactome release 97 curated reaction event. The source record connects dGMP, H2O to 2DORP, Gua in cytosol and cross-references RHEA:57817.", "equation": "dGMP + H2O → 2DORP + Gua", "inputs": [ { "node_id": "R-ALL-109327", "reactome_id": "R-ALL-109327", "label": "dGMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57673", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8953344", "reactome_id": "R-ALL-8953344", "label": "2DORP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62877", "formula": "C5H9O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-83956", "reactome_id": "R-ALL-83956", "label": "Gua", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16235", "formula": "C5H5N5O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "5-hydroxymethyl-dUMP N-hydrolase activity of DNPH1 [cytosol]", "activity_id": "8953366", "ec_numbers": [ "3.2.2" ], "accessions": [ "O43598" ] } ], "citations": [ { "id": "PMID:24260472", "title": "N (6)-substituted AMPs inhibit mammalian deoxynucleotide N-hydrolase DNPH1", "journal": "PLoS ONE", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24260472/" }, { "id": "PMID:17234634", "title": "The c-Myc target gene Rcl (C6orf108) encodes a novel enzyme, deoxynucleoside 5'-monophosphate N-glycosidase", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17234634/" }, { "id": "PMID:25108359", "title": "6-(Hetero)Arylpurine nucleotides as inhibitors of the oncogenic target DNPH1: synthesis, structural studies and cytotoxic activities", "journal": "Eur J Med Chem", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25108359/" } ], "x": 2660, "y": 3167.0833333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8953430", "label": "PXMP2 trimer transports glycolate from cytosol to peroxisomal matrix", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "peroxisomal membrane", "compartment_ids": [ "GO:0005778" ], "rhea_ids": [ "RHEA:29447" ], "source_url": "https://reactome.org/content/detail/R-HSA-8953430", "summary": "PXMP2 trimer transports glycolate from cytosol to peroxisomal matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects glycolate to glycolate in peroxisomal membrane and cross-references RHEA:29447.", "equation": "glycolate → glycolate", "inputs": [ { "node_id": "R-ALL-8953440", "reactome_id": "R-ALL-8953440", "label": "glycolate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29805", "formula": "C2H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389822", "reactome_id": "R-ALL-389822", "label": "glycolate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29805", "formula": "C2H3O3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "channel activity of PXMP2 trimer [peroxisomal membrane]", "activity_id": "8953438", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:19352492", "title": "Pxmp2 is a channel-forming protein in Mammalian peroxisomal membrane", "journal": "PLoS ONE", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19352492/" }, { "id": "PMID:26858947", "title": "Metabolic Interplay between Peroxisomes and Other Subcellular Organelles Including Mitochondria and the Endoplasmic Reticulum", "journal": "Front Cell Dev Biol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26858947/" } ], "x": 868.6666666666667, "y": 3237, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8953499", "label": "IMPAD1 hydrolyses PAP to AMP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "Golgi lumen", "compartment_ids": [ "GO:0005796" ], "rhea_ids": [ "RHEA:10041" ], "source_url": "https://reactome.org/content/detail/R-HSA-8953499", "summary": "IMPAD1 hydrolyses PAP to AMP is a Reactome release 97 curated reaction event. The source record connects PAP, H2O to adenosine 5'-monophosphate, Pi in Golgi lumen and cross-references RHEA:10041.", "equation": "PAP + H2O → adenosine 5'-monophosphate + Pi", "inputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2022884", "reactome_id": "R-ALL-2022884", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8953493", "reactome_id": "R-ALL-8953493", "label": "adenosine 5'-monophosphate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8851226", "reactome_id": "R-ALL-8851226", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3'(2'),5'-bisphosphate nucleotidase activity of IMPAD1:Mg2+ [Golgi lumen]", "activity_id": "8953492", "ec_numbers": [ "3.1.3.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:23479625", "title": "Role for cytoplasmic nucleotide hydrolysis in hepatic function and protein synthesis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23479625/" } ], "x": 7461.333333333333, "y": 2928.8529411764707, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8954262", "label": "TPST1,2 transfer SO4(2-) from PAPS to PODXL2", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "Golgi lumen · Golgi membrane", "compartment_ids": [ "GO:0005796", "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8954262", "summary": "TPST1,2 transfer SO4(2-) from PAPS to PODXL2 is a Reactome release 97 curated reaction event. The source record connects PODXL2, PAPS to OSulf-Y97,118-PODXL2, PAP in Golgi lumen · Golgi membrane.", "equation": "PODXL2 + 2 PAPS → OSulf-Y97,118-PODXL2 + 2 PAP", "inputs": [ { "node_id": "R-HSA-8954248", "reactome_id": "R-HSA-8954248", "label": "PODXL2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8954273", "reactome_id": "R-HSA-8954273", "label": "OSulf-Y97,118-PODXL2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "protein-tyrosine sulfotransferase activity of TPST1,2 [Golgi membrane]", "activity_id": "8954238", "ec_numbers": [ "2.8.2.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:12889478", "title": "Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl Lewis x", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12889478/" }, { "id": "PMID:18606703", "title": "Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins", "journal": "J. Immunol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18606703/" }, { "id": "PMID:18672380", "title": "Mass spectrometric kinetic analysis of human tyrosylprotein sulfotransferase-1 and -2", "journal": "J. Am. Soc. Mass Spectrom.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18672380/" }, { "id": "PMID:23481380", "title": "Crystal structure of human tyrosylprotein sulfotransferase-2 reveals the mechanism of protein tyrosine sulfation reaction", "journal": "Nat Commun", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23481380/" }, { "id": "PMID:9501187", "title": "Tyrosylprotein sulfotransferase: purification and molecular cloning of an enzyme that catalyzes tyrosine O-sulfation, a common posttranslational modification of eukaryotic proteins", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9501187/" } ], "x": 7740, "y": 3134.794117647059, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8954327", "label": "UCKL1 phosphorylates urindine, cytidine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage", "pyrimidine-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24675", "RHEA:16826" ], "source_url": "https://reactome.org/content/detail/R-HSA-8954327", "summary": "UCKL1 phosphorylates urindine, cytidine is a Reactome release 97 curated reaction event. The source record connects Cyt-Rib, Ura-Rib, ATP to CMP, UMP, ADP, H+ in cytosol and cross-references RHEA:24675, RHEA:16826.", "equation": "Cyt-Rib, Ura-Rib + ATP → CMP, UMP + ADP + H+", "inputs": [ { "node_id": "R-ALL-500759", "reactome_id": "R-ALL-500759", "label": "Cyt-Rib, Ura-Rib", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-500761", "reactome_id": "R-ALL-500761", "label": "CMP, UMP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "uridine kinase activity of UCKL1 [cytosol]", "activity_id": "8954329", "ec_numbers": [ "2.7.1.48" ], "accessions": [ "Q9NWZ5" ] } ], "citations": [ { "id": "PMID:12199906", "title": "Epstein-Barr virus encoded nuclear protein EBNA-3 binds a novel human uridine kinase/uracil phosphoribosyltransferase", "journal": "BMC Cell Biol.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12199906/" }, { "id": "PMID:195585", "title": "Uridine kinase activities and pyrimidine nucleoside phosphorylation in fluoropyrimidine-sensitive and -resistant cell lines of the Novikoff hepatoma", "journal": "Biochem J", "year": 1977, "url": "https://pubmed.ncbi.nlm.nih.gov/195585/" }, { "id": "PMID:11306702", "title": "Phosphorylation of uridine and cytidine nucleoside analogs by two human uridine-cytidine kinases", "journal": "Mol Pharmacol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11306702/" } ], "x": 3600, "y": 2899.1071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8954398", "label": "PLD6 dimer hydrolyses cardiolipin to PA and PG", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "mitochondrial outer membrane · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005741", "GO:0005758" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8954398", "summary": "PLD6 dimer hydrolyses cardiolipin to PA and PG is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects cardiolipin, H2O to PA, PG in mitochondrial outer membrane · mitochondrial intermembrane space.", "equation": "cardiolipin + H2O → PA + PG", "inputs": [ { "node_id": "R-ALL-8954419", "reactome_id": "R-ALL-8954419", "label": "cardiolipin", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-189422", "reactome_id": "R-ALL-189422", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1500590", "reactome_id": "R-ALL-1500590", "label": "PA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57739", "formula": "C5H5O8PR2", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-8954401", "reactome_id": "R-ALL-8954401", "label": "PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64716", "formula": "C8H12O10PR2", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cardiolipin phospholipase D activity of PLD6 dimer [mitochondrial outer membrane]", "activity_id": "8954400", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:17028579", "title": "A common lipid links Mfn-mediated mitochondrial fusion and SNARE-regulated exocytosis", "journal": "Nat. Cell Biol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17028579/" }, { "id": "PMID:14573790", "title": "Phospholipid scramblase 3 controls mitochondrial structure, function, and apoptotic response", "journal": "Mol. Cancer Res.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14573790/" }, { "id": "PMID:26711011", "title": "Mitoguardin Regulates Mitochondrial Fusion through MitoPLD and Is Required for Neuronal Homeostasis", "journal": "Mol. Cell", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26711011/" } ], "x": 2938.666666666667, "y": 1844.7328767123288, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8955030", "label": "NADK2 dimer phosphorylates NAD+ to NADP+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18630" ], "source_url": "https://reactome.org/content/detail/R-HSA-8955030", "summary": "NADK2 dimer phosphorylates NAD+ to NADP+ is a Reactome release 97 curated reaction event. The source record connects NAD+, ATP to NADP+, ADP, H+ in mitochondrial matrix and cross-references RHEA:18630.", "equation": "NAD+ + ATP → NADP+ + ADP + H+", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NAD+ kinase activity of NADK2 dimer [mitochondrial matrix]", "activity_id": "8955033", "ec_numbers": [ "2.7.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:23212377", "title": "Identification and characterization of a human mitochondrial NAD kinase", "journal": "Nat Commun", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23212377/" }, { "id": "PMID:24847004", "title": "Mitochondrial NADP(H) deficiency due to a mutation in NADK2 causes dienoyl-CoA reductase deficiency with hyperlysinemia", "journal": "Hum. Mol. Genet.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24847004/" } ], "x": 5670, "y": 2859.3026315789475, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8955760", "label": "PGM2L1:Mg2+ phosphorylates G6P to G1,6BP", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycolysis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:70912" ], "source_url": "https://reactome.org/content/detail/R-HSA-8955760", "summary": "PGM2L1:Mg2+ phosphorylates G6P to G1,6BP is a Reactome release 97 curated reaction event. The source record connects G6P, 1,3BPG to G1,6BP, 3PG, H+ in cytosol and cross-references RHEA:70912.", "equation": "G6P + 1,3BPG → G1,6BP + 3PG + H+", "inputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29800", "reactome_id": "R-ALL-29800", "label": "1,3BPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57604", "formula": "C3H4O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8955759", "reactome_id": "R-ALL-8955759", "label": "G1,6BP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58392", "formula": "C6H10O12P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29728", "reactome_id": "R-ALL-29728", "label": "3PG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58272", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucose-1,6-bisphosphate synthase activity of PGM2L1:Mg2+ [cytosol]", "activity_id": "8955758", "ec_numbers": [ "2.7.1.106" ], "accessions": [] } ], "citations": [ { "id": "PMID:18927083", "title": "Mammalian phosphomannomutase PMM1 is the brain IMP-sensitive glucose-1,6-bisphosphatase", "journal": "J. Biol. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18927083/" }, { "id": "PMID:17804405", "title": "Molecular identification of mammalian phosphopentomutase and glucose-1,6-bisphosphate synthase, two members of the alpha-D-phosphohexomutase family", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17804405/" } ], "x": 590, "y": 335.9375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8955794", "label": "PGP:Mg2+ dimer hydrolyses G3P to glycerol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:66373" ], "source_url": "https://reactome.org/content/detail/R-HSA-8955794", "summary": "PGP:Mg2+ dimer hydrolyses G3P to glycerol is a Reactome release 97 curated reaction event. The source record connects H2O, G3P to Glycerol, Pi in cytosol and cross-references RHEA:66373.", "equation": "H2O + G3P → Glycerol + Pi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76114", "reactome_id": "R-ALL-76114", "label": "G3P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57597", "formula": "C3H7O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76116", "reactome_id": "R-ALL-76116", "label": "Glycerol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17754", "formula": "C3H8O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sn-glycerol 3-phosphatase activity of PGP:Mg2+ dimer [cytosol]", "activity_id": "8955800", "ec_numbers": [ "3.1.3.21" ], "accessions": [] } ], "citations": [ { "id": "PMID:35272070", "title": "Glycerol-3-phosphate phosphatase operates a glycerol shunt in pancreatic β-cells that controls insulin secretion and metabolic stress", "journal": "Mol Metab", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35272070/" }, { "id": "PMID:36198384", "title": "Hepatic glycerol shunt and glycerol-3-phosphate phosphatase control liver metabolism and glucodetoxification under hyperglycemia", "journal": "Mol Metab", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36198384/" }, { "id": "PMID:26755581", "title": "Identification of a mammalian glycerol-3-phosphate phosphatase: Role in metabolism and signaling in pancreatic β-cells and hepatocytes", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26755581/" }, { "id": "PMID:39542419", "title": "Essential role of germ cell glycerol-3-phosphate phosphatase for sperm health, oxidative stress control and male fertility in mice", "journal": "Mol Metab", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/39542419/" }, { "id": "PMID:24338473", "title": "Evolutionary and structural analyses of mammalian haloacid dehalogenase-type phosphatases AUM and chronophin provide insight into the basis of their different substrate specificities", "journal": "J Biol Chem", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24338473/" } ], "x": 2660, "y": 1535.664383561644, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8955817", "label": "PRODH2 oxidises HPRO to 1PYR-3OH-5COOH", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "proline" ], "compartment": "mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:52513" ], "source_url": "https://reactome.org/content/detail/R-HSA-8955817", "summary": "PRODH2 oxidises HPRO to 1PYR-3OH-5COOH is a Reactome release 97 curated reaction event. The source record connects HPRO, FAD to FADH2, H2O, 1PYR-3OH-5COOH in mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:52513.", "equation": "HPRO + FAD → FADH2 + H2O + 1PYR-3OH-5COOH", "inputs": [ { "node_id": "R-ALL-6784215", "reactome_id": "R-ALL-6784215", "label": "HPRO", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58375", "formula": "C5H9NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113597", "reactome_id": "R-ALL-113597", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-164934", "reactome_id": "R-ALL-164934", "label": "FADH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58307", "formula": "C27H33N9O15P2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9929425", "reactome_id": "R-ALL-9929425", "label": "1PYR-3OH-5COOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62612", "formula": "C5H6NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "proline dehydrogenase activity of PRODH2 [mitochondrial inner membrane]", "activity_id": "8955819", "ec_numbers": [ "1.5.5.2" ], "accessions": [ "Q9UF12" ] } ], "citations": [ { "id": "PMID:25697095", "title": "Proline dehydrogenase 2 (PRODH2) is a hydroxyproline dehydrogenase (HYPDH) and molecular target for treating primary hyperoxaluria", "journal": "Biochem. J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25697095/" } ], "x": 6800, "y": 2363.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8955844", "label": "RBKS phosphorylates ribose to R5P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13698" ], "source_url": "https://reactome.org/content/detail/R-HSA-8955844", "summary": "RBKS phosphorylates ribose to R5P is a Reactome release 97 curated reaction event. The source record connects D-ribose, ATP to R5P, ADP, H+ in cytosol and cross-references RHEA:13698.", "equation": "D-ribose + ATP → R5P + ADP + H+", "inputs": [ { "node_id": "R-ALL-8955853", "reactome_id": "R-ALL-8955853", "label": "D-ribose", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:47013", "formula": "C5H10O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73578", "reactome_id": "R-ALL-73578", "label": "R5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78346", "formula": "C5H9O8P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ribokinase activity of RBKS [cytosol]", "activity_id": "8955849", "ec_numbers": [ "2.7.1.15" ], "accessions": [ "Q9H477" ] } ], "citations": [ { "id": "PMID:17585908", "title": "Identification and characterization of human ribokinase and comparison of its properties with E. coli ribokinase and human adenosine kinase", "journal": "FEBS Lett.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17585908/" }, { "id": "PMID:18560757", "title": "Adenosine kinase and ribokinase--the RK family of proteins", "journal": "Cell. Mol. Life Sci.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18560757/" } ], "x": 868.6666666666667, "y": 769.7857142857143, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8956458", "label": "RNLS:FAD oxidises dh-beta-NAD to NAD+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [ "RHEA:40396", "RHEA:47997" ], "source_url": "https://reactome.org/content/detail/R-HSA-8956458", "summary": "RNLS:FAD oxidises dh-beta-NAD to NAD+ is a Reactome release 97 curated reaction event. The source record connects dh-beta-NAD, O2, H+ to NAD+, H2O2 in extracellular region and cross-references RHEA:40396, RHEA:47997.", "equation": "dh-beta-NAD + O2 + H+ → NAD+ + H2O2", "inputs": [ { "node_id": "R-ALL-8956473", "reactome_id": "R-ALL-8956473", "label": "dh-beta-NAD", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-352327", "reactome_id": "R-ALL-352327", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-352330", "reactome_id": "R-ALL-352330", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-351629", "reactome_id": "R-ALL-351629", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on NAD(P)H of RNLS:FAD [extracellular region]", "activity_id": "8956470", "ec_numbers": [ "1.6" ], "accessions": [] } ], "citations": [ { "id": "PMID:21699903", "title": "FAD-binding site and NADP reactivity in human renalase: a new enzyme involved in blood pressure regulation", "journal": "J. Mol. Biol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21699903/" }, { "id": "PMID:25531177", "title": "Metabolic function for human renalase: oxidation of isomeric forms of β-NAD(P)H that are inhibitory to primary metabolism", "journal": "Biochemistry", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25531177/" } ], "x": 5948.666666666667, "y": 2869.5394736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8957389", "label": "RPP14 (HTD2) dehydrates 3HA-CoA to t2E-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:26527" ], "source_url": "https://reactome.org/content/detail/R-HSA-8957389", "summary": "RPP14 (HTD2) dehydrates 3HA-CoA to t2E-CoA is a Reactome release 97 curated reaction event. The source record connects 3HA-CoA to t2E-CoA, H2O in mitochondrial matrix and cross-references RHEA:26527.", "equation": "3HA-CoA → t2E-CoA + H2O", "inputs": [ { "node_id": "R-ALL-8957387", "reactome_id": "R-ALL-8957387", "label": "3HA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57319", "formula": "C24H35N7O18P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8957386", "reactome_id": "R-ALL-8957386", "label": "t2E-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58856", "formula": "C24H33N7O17P3SR", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-hydroxyacyl-CoA dehydratase activity of HTD2 [mitochondrial matrix]", "activity_id": "8957385", "ec_numbers": [ "4.2.1.150" ], "accessions": [ "P86397" ] } ], "citations": [ { "id": "PMID:17898086", "title": "An ancient genetic link between vertebrate mitochondrial fatty acid synthesis and RNA processing", "journal": "FASEB J.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17898086/" } ], "x": 868.6666666666667, "y": 1688.4035087719299, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8959462", "label": "SAMD8 transfers phosphatidyl from PE onto C16DH CER", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:42128" ], "source_url": "https://reactome.org/content/detail/R-HSA-8959462", "summary": "SAMD8 transfers phosphatidyl from PE onto C16DH CER is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PE, C16DH CER to CPE, DAG in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:42128.", "equation": "PE + C16DH CER → CPE + DAG", "inputs": [ { "node_id": "R-ALL-9843247", "reactome_id": "R-ALL-9843247", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64612", "formula": "C7H12NO8PR2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9843232", "reactome_id": "R-ALL-9843232", "label": "C16DH CER", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:67042", "formula": "C34H69NO3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8959463", "reactome_id": "R-ALL-8959463", "label": "CPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78654", "formula": "C36H75N2O6P", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1500596", "reactome_id": "R-ALL-1500596", "label": "DAG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17815", "formula": "C5H6O5R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ceramide phosphoethanolamine synthase activity of SAMD8 trimer [endoplasmic reticulum membrane]", "activity_id": "8959466", "ec_numbers": [ "2.7.8.48" ], "accessions": [] } ], "citations": [ { "id": "PMID:19506037", "title": "Sphingomyelin synthase-related protein SMSr controls ceramide homeostasis in the ER", "journal": "J. Cell Biol.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19506037/" }, { "id": "PMID:31067435", "title": "Ceramide phosphoethanolamine, an enigmatic cellular membrane sphingolipid", "journal": "Biochim Biophys Acta Biomembr", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31067435/" }, { "id": "PMID:28120887", "title": "ER residency of the ceramide phosphoethanolamine synthase SMSr relies on homotypic oligomerization mediated by its SAM domain", "journal": "Sci Rep", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28120887/" } ], "x": 3878.666666666667, "y": 1645.2115384615386, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-8959719", "label": "SHPK phosphorylates Sedo to Sedo7P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23845" ], "source_url": "https://reactome.org/content/detail/R-HSA-8959719", "summary": "SHPK phosphorylates Sedo to Sedo7P is a Reactome release 97 curated reaction event. The source record connects Sedo, ATP to SH7P, ADP, H+ in cytosol and cross-references RHEA:23845.", "equation": "Sedo + ATP → SH7P + ADP + H+", "inputs": [ { "node_id": "R-ALL-8959707", "reactome_id": "R-ALL-8959707", "label": "Sedo", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16802", "formula": "C7H14O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29882", "reactome_id": "R-ALL-29882", "label": "SH7P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57483", "formula": "C7H13O10P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sedoheptulokinase activity of SHPK [cytosol]", "activity_id": "8959703", "ec_numbers": [ "2.7.1.14" ], "accessions": [ "Q9UHJ6" ] } ], "citations": [ { "id": "PMID:22682222", "title": "The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism", "journal": "Cell Metab.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22682222/" }, { "id": "PMID:18186520", "title": "Sedoheptulokinase deficiency due to a 57-kb deletion in cystinosis patients causes urinary accumulation of sedoheptulose: elucidation of the CARKL gene", "journal": "Hum. Mutat.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18186520/" }, { "id": "PMID:18775706", "title": "Characterization of mammalian sedoheptulokinase and mechanism of formation of erythritol in sedoheptulokinase deficiency", "journal": "FEBS Lett.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18775706/" } ], "x": 311.33333333333337, "y": 825.3571428571429, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8963851", "label": "VD3 dissociates from GC", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8963851", "summary": "VD3 dissociates from GC is a Reactome release 97 curated reaction event. The source record connects GC:VD3 to GC, VD3 in extracellular region.", "equation": "GC:VD3 → GC + VD3", "inputs": [ { "node_id": "R-HSA-352339", "reactome_id": "R-HSA-352339", "label": "GC:VD3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-209886", "reactome_id": "R-HSA-209886", "label": "GC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-209767", "reactome_id": "R-ALL-209767", "label": "VD3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28940", "formula": "C27H44O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of GC:VD3 [extracellular region]", "activity_id": "9959972", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11799400", "title": "A structural basis for the unique binding features of the human vitamin D-binding protein", "journal": "Nat Struct Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11799400/" } ], "x": 5008.666666666667, "y": 1748.128787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8963872", "label": "VD3 translocates from ER membrane to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "extracellular region · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005576", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8963872", "summary": "VD3 translocates from ER membrane to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects VD3 to VD3 in extracellular region · endoplasmic reticulum membrane.", "equation": "VD3 → VD3", "inputs": [ { "node_id": "R-ALL-8963870", "reactome_id": "R-ALL-8963870", "label": "VD3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28940", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-209767", "reactome_id": "R-ALL-209767", "label": "VD3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28940", "formula": "C27H44O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11799400", "title": "A structural basis for the unique binding features of the human vitamin D-binding protein", "journal": "Nat Struct Biol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11799400/" } ], "x": 5008.666666666667, "y": 1860.1136363636363, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-8963913", "label": "1,25(OH)2D translocates from cytosol to nucleoplasm", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · nucleoplasm", "compartment_ids": [ "GO:0005829", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8963913", "summary": "1,25(OH)2D translocates from cytosol to nucleoplasm is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 1,25(OH)2D to 1,25(OH)2D in cytosol · nucleoplasm.", "equation": "1,25(OH)2D → 1,25(OH)2D", "inputs": [ { "node_id": "R-ALL-209800", "reactome_id": "R-ALL-209800", "label": "1,25(OH)2D", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17823", "formula": "C27H44O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8963912", "reactome_id": "R-ALL-8963912", "label": "1,25(OH)2D", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17823", "formula": "C27H44O3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:26681795", "title": "Vitamin D: Metabolism, Molecular Mechanism of Action, and Pleiotropic Effects", "journal": "Physiol. Rev.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26681795/" } ], "x": 4451.333333333333, "y": 1866.0075757575758, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-8963915", "label": "1,25(OH)2D binds VDR", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-8963915", "summary": "1,25(OH)2D binds VDR is a Reactome release 97 curated reaction event. The source record connects 1,25(OH)2D, VDR to 1,25(OH)2D:VDR in nucleoplasm.", "equation": "1,25(OH)2D + VDR → 1,25(OH)2D:VDR", "inputs": [ { "node_id": "R-ALL-8963912", "reactome_id": "R-ALL-8963912", "label": "1,25(OH)2D", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17823", "formula": "C27H44O3", "compartment": "nucleoplasm", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-375512", "reactome_id": "R-HSA-375512", "label": "VDR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-8963916", "reactome_id": "R-HSA-8963916", "label": "1,25(OH)2D:VDR", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "nuclear steroid receptor activity of VDR [nucleoplasm]", "activity_id": "9959974", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15728261", "title": "Superagonistic action of 14-epi-analogs of 1,25-dihydroxyvitamin D explained by vitamin D receptor-coactivator interaction", "journal": "Mol. Pharmacol.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15728261/" }, { "id": "PMID:15647825", "title": "1,25-Dihydroxyvitamin D3 stimulates cyclic vitamin D receptor/retinoid X receptor DNA-binding, co-activator recruitment, and histone acetylation in intact osteoblasts", "journal": "J. Bone Miner. Res.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15647825/" }, { "id": "PMID:28206978", "title": "Pathogenic roles of alterations in vitamin D and vitamin D receptor in gastric tumorigenesis", "journal": "Oncotarget", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28206978/" }, { "id": "PMID:22877430", "title": "Extraskeletal effects of vitamin D", "journal": "Endocrinol. Metab. Clin. North Am.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22877430/" }, { "id": "PMID:23857223", "title": "Vitamin D and immune function", "journal": "Nutrients", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23857223/" }, { "id": "PMID:21849106", "title": "Vitamin D and immune function: an overview", "journal": "Proc Nutr Soc", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/21849106/" } ], "x": 4451.333333333333, "y": 1754.0227272727273, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-8982667", "label": "PGM2:Mg2+ isomerises dR1P to dR5P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "pentose-phosphate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:27659" ], "source_url": "https://reactome.org/content/detail/R-HSA-8982667", "summary": "PGM2:Mg2+ isomerises dR1P to dR5P is a Reactome release 97 curated reaction event. The source record connects dR1P to dR5P in cytosol and cross-references RHEA:27659.", "equation": "dR1P → dR5P", "inputs": [ { "node_id": "R-ALL-6787326", "reactome_id": "R-ALL-6787326", "label": "dR1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57259", "formula": "C5H9O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-6787328", "reactome_id": "R-ALL-6787328", "label": "dR5P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:62877", "formula": "C5H9O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphopentomutase activity of PGM2:Mg2+ [cytosol]", "activity_id": "6787332", "ec_numbers": [ "5.4.2.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:17804405", "title": "Molecular identification of mammalian phosphopentomutase and glucose-1,6-bisphosphate synthase, two members of the alpha-D-phosphohexomutase family", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17804405/" } ], "x": 590, "y": 992.0714285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-901097", "label": "PXLP-KYAT3 (CCBL2) dimer transaminates L-KYN to KYNA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:65561" ], "source_url": "https://reactome.org/content/detail/R-HSA-901097", "summary": "PXLP-KYAT3 (CCBL2) dimer transaminates L-KYN to KYNA is a Reactome release 97 curated reaction event. The source record connects L-KYN, 2OG to KYNA, L-Glu, H2O in cytosol and cross-references RHEA:65561.", "equation": "L-KYN + 2OG → KYNA + L-Glu + H2O", "inputs": [ { "node_id": "R-ALL-29970", "reactome_id": "R-ALL-29970", "label": "L-KYN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57959", "formula": "C10H12N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-893587", "reactome_id": "R-ALL-893587", "label": "KYNA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58454", "formula": "C10H6NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-kynurenine:2-oxoglutarate transaminase activity of CCBL2 dimer [cytosol]", "activity_id": "901096", "ec_numbers": [ "2.6.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:16376499", "title": "Characterization of kynurenine aminotransferase III, a novel member of a phylogenetically conserved KAT family", "journal": "Gene", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16376499/" } ], "x": 7461.333333333333, "y": 1834.7916666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9011543", "label": "DCA binds PDK2", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9011543", "summary": "DCA binds PDK2 is a Reactome release 97 curated reaction event. The source record connects DCA, PDK2(?-407) to DCA:PDK2 in mitochondrial matrix.", "equation": "DCA + PDK2(?-407) → DCA:PDK2", "inputs": [ { "node_id": "R-ALL-9010792", "reactome_id": "R-ALL-9010792", "label": "DCA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "ChemicalDrug", "currency": false }, { "node_id": "R-HSA-203943", "reactome_id": "R-HSA-203943", "label": "PDK2(?-407)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9011531", "reactome_id": "R-HSA-9011531", "label": "DCA:PDK2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19833728", "title": "Pivotal role of the C-terminal DW-motif in mediating inhibition of pyruvate dehydrogenase kinase 2 by dichloroacetate", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19833728/" }, { "id": "PMID:17544412", "title": "Amino acid residues responsible for the recognition of dichloroacetate by pyruvate dehydrogenase kinase 2", "journal": "FEBS Lett.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17544412/" } ], "x": 4730, "y": 593.65, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9011595", "label": "GSTZ1 dimer dehalogenates DCA", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:16438" ], "source_url": "https://reactome.org/content/detail/R-HSA-9011595", "summary": "GSTZ1 dimer dehalogenates DCA is a Reactome release 97 curated reaction event. The source record connects DCA, GSH to GS-MCA, Cl-, H+ in mitochondrial matrix and cross-references RHEA:16438.", "equation": "DCA + GSH → GS-MCA + Cl- + H+", "inputs": [ { "node_id": "R-ALL-9010792", "reactome_id": "R-ALL-9010792", "label": "DCA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "ChemicalDrug", "currency": false }, { "node_id": "R-ALL-1655887", "reactome_id": "R-ALL-1655887", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16856", "formula": "C10H17N3O6S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9861569", "reactome_id": "R-ALL-9861569", "label": "GS-MCA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:229639", "formula": "C12H17ClN3O8S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9861558", "reactome_id": "R-ALL-9861558", "label": "Cl-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17996", "formula": "Cl", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glutathione transferase activity of GSTZ1 dimer [mitochondrial matrix]", "activity_id": "9011598", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:20884751", "title": "Mitochondrion as a novel site of dichloroacetate biotransformation by glutathione transferase zeta 1", "journal": "J. Pharmacol. Exp. Ther.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/20884751/" }, { "id": "PMID:9710704", "title": "Pharmacokinetics, metabolism and toxicology of dichloroacetate", "journal": "Drug Metab. Rev.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9710704/" }, { "id": "PMID:11327815", "title": "Crystal structure of maleylacetoacetate isomerase/glutathione transferase zeta reveals the molecular basis for its remarkable catalytic promiscuity.", "journal": "Biochemistry", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11327815/" }, { "id": "PMID:24637752", "title": "Preliminary X-ray crystallographic analysis of glutathione transferase zeta 1 (GSTZ1a-1a)", "journal": "Acta Crystallogr F Struct Biol Commun", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24637752/" }, { "id": "PMID:9815194", "title": "Glutathione transferase zeta-catalyzed biotransformation of dichloroacetic acid and other alpha-haloacids", "journal": "Chem Res Toxicol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9815194/" } ], "x": 5008.666666666667, "y": 399.15, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9012016", "label": "FAHD1 dimer hydrolyses OA to PYR", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15642" ], "source_url": "https://reactome.org/content/detail/R-HSA-9012016", "summary": "FAHD1 dimer hydrolyses OA to PYR is a Reactome release 97 curated reaction event. The source record connects OA, H+ to PYR, CO2 in mitochondrial matrix and cross-references RHEA:15642.", "equation": "OA + H+ → PYR + CO2", "inputs": [ { "node_id": "R-ALL-113587", "reactome_id": "R-ALL-113587", "label": "OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxaloacetate decarboxylase activity of FAHD1:Mg2+ dimer [mitochondrial matrix]", "activity_id": "9012042", "ec_numbers": [ "4.1.1.112" ], "accessions": [] } ], "citations": [ { "id": "PMID:15551868", "title": "X-ray structure of fumarylacetoacetate hydrolase family member Homo sapiens FLJ36880", "journal": "Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15551868/" }, { "id": "PMID:25575590", "title": "Identification of FAH domain-containing protein 1 (FAHD1) as oxaloacetate decarboxylase", "journal": "J. Biol. Chem.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25575590/" } ], "x": 5008.666666666667, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9012036", "label": "ME1 tetramer decarboxylates MAL to PYR", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:18254" ], "source_url": "https://reactome.org/content/detail/R-HSA-9012036", "summary": "ME1 tetramer decarboxylates MAL to PYR is a Reactome release 97 curated reaction event. The source record connects MAL, NADP+ to PYR, CO2, NADPH in cytosol and cross-references RHEA:18254.", "equation": "MAL + NADP+ → PYR + CO2 + NADPH + H+", "inputs": [ { "node_id": "R-ALL-198498", "reactome_id": "R-ALL-198498", "label": "MAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "malate dehydrogenase (decarboxylating) (NADP+) activity of ME1 tetramer [cytosol]", "activity_id": "9012039", "ec_numbers": [ "1.1.1.40" ], "accessions": [] } ], "citations": [ { "id": "PMID:1935931", "title": "Malic enzyme in human liver. Intracellular distribution, purification and properties of cytosolic isozyme", "journal": "Eur. J. Biochem.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1935931/" }, { "id": "PMID:14596586", "title": "Structure and function of malic enzymes, a new class of oxidative decarboxylases", "journal": "Biochemistry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14596586/" }, { "id": "PMID:24998673", "title": "Structural characteristics of the nonallosteric human cytosolic malic enzyme", "journal": "Biochim. Biophys. Acta", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24998673/" }, { "id": "PMID:23284632", "title": "Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23284632/" }, { "id": "PMID:33064660", "title": "Malic enzyme 1 (ME1) in the biology of cancer: it is not just intermediary metabolism", "journal": "J Mol Endocrinol", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33064660/" }, { "id": "PMID:7757881", "title": "Purification and properties of cytosolic and mitochondrial malic enzyme isolated from human brain", "journal": "Int J Biochem Cell Biol", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7757881/" } ], "x": 4451.333333333333, "y": 282.45, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9012268", "label": "ME2 tetramer decarboxylates MAL to PYR", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:12654" ], "source_url": "https://reactome.org/content/detail/R-HSA-9012268", "summary": "ME2 tetramer decarboxylates MAL to PYR is a Reactome release 97 curated reaction event. The source record connects MAL, NAD+ to PYR, CO2, NADH in mitochondrial matrix and cross-references RHEA:12654.", "equation": "MAL + NAD+ → PYR + CO2 + NADH", "inputs": [ { "node_id": "R-ALL-113544", "reactome_id": "R-ALL-113544", "label": "MAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "malate dehydrogenase (decarboxylating) (NAD+) activity of ME2:Mg2+ tetramer [mitochondrial matrix]", "activity_id": "9012261", "ec_numbers": [ "1.1.1.39" ], "accessions": [] } ], "citations": [ { "id": "PMID:1993674", "title": "Human NAD(+)-dependent mitochondrial malic enzyme. cDNA cloning, primary structure, and expression in Escherichia coli", "journal": "J. Biol. Chem.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1993674/" }, { "id": "PMID:12962632", "title": "Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism", "journal": "Structure", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12962632/" }, { "id": "PMID:14596586", "title": "Structure and function of malic enzymes, a new class of oxidative decarboxylases", "journal": "Biochemistry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14596586/" }, { "id": "PMID:23284632", "title": "Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23284632/" }, { "id": "PMID:12121650", "title": "Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate", "journal": "Structure", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12121650/" }, { "id": "PMID:25202825", "title": "Human mitochondrial NAD(P)(+)-dependent malic enzyme participates in cutaneous melanoma progression and invasion", "journal": "J. Invest. Dermatol.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25202825/" } ], "x": 4730, "y": 321.35, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9012349", "label": "ME3 tetramer decarboxylates MAL to PYR", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18254" ], "source_url": "https://reactome.org/content/detail/R-HSA-9012349", "summary": "ME3 tetramer decarboxylates MAL to PYR is a Reactome release 97 curated reaction event. The source record connects MAL, NADP+ to PYR, CO2, NADPH in mitochondrial matrix and cross-references RHEA:18254.", "equation": "MAL + NADP+ → PYR + CO2 + NADPH", "inputs": [ { "node_id": "R-ALL-113544", "reactome_id": "R-ALL-113544", "label": "MAL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113563", "reactome_id": "R-ALL-113563", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113600", "reactome_id": "R-ALL-113600", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "malate dehydrogenase (decarboxylating) (NADP+) activity of ME3:Mg2+ tetramer [mitochondrial matrix]", "activity_id": "9012354", "ec_numbers": [ "1.1.1.40" ], "accessions": [] } ], "citations": [ { "id": "PMID:7818469", "title": "Purification, cDNA cloning and heterologous expression of the human mitochondrial NADP(+)-dependent malic enzyme", "journal": "Biochem. J.", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7818469/" }, { "id": "PMID:14596586", "title": "Structure and function of malic enzymes, a new class of oxidative decarboxylases", "journal": "Biochemistry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14596586/" }, { "id": "PMID:25594249", "title": "Mitochondrial malic enzyme 3 is important for insulin secretion in pancreatic β-cells", "journal": "Mol. Endocrinol.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25594249/" }, { "id": "PMID:23284632", "title": "Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23284632/" }, { "id": "PMID:36590518", "title": "Integrative structural and functional analysis of human malic enzyme 3: A potential therapeutic target for pancreatic cancer", "journal": "Heliyon", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36590518/" }, { "id": "PMID:33572903", "title": "The Pancreatic β-Cell: The Perfect Redox System", "journal": "Antioxidants (Basel)", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33572903/" } ], "x": 5008.666666666667, "y": 321.35, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9012374", "label": "VDAC1 transports PYR from cytosol to mitochondrial intermembrane space", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol · mitochondrial intermembrane space · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005758", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9012374", "summary": "VDAC1 transports PYR from cytosol to mitochondrial intermembrane space is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PYR to PYR in cytosol · mitochondrial intermembrane space · mitochondrial outer membrane.", "equation": "PYR → PYR", "inputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9012375", "reactome_id": "R-ALL-9012375", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "voltage-gated monoatomic anion channel activity of VDAC1 [mitochondrial outer membrane]", "activity_id": "9012385", "ec_numbers": null, "accessions": [ "P21796" ] } ], "citations": [ { "id": "PMID:8726225", "title": "Deficiency of the voltage-dependent anion channel: a novel cause of mitochondriopathy", "journal": "Pediatr. Res.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8726225/" }, { "id": "PMID:21156174", "title": "Biphasic effect of nitric oxide on the cardiac voltage-dependent anion channel", "journal": "FEBS Lett", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21156174/" }, { "id": "PMID:35164499", "title": "Structure and Gating Behavior of the Human Integral Membrane Protein VDAC1 in a Lipid Bilayer", "journal": "J Am Chem Soc", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35164499/" }, { "id": "PMID:34359907", "title": "The Role of Voltage-Dependent Anion Channel in Mitochondrial Dysfunction and Human Disease", "journal": "Cells", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34359907/" }, { "id": "PMID:25748677", "title": "Mitochondrial pyruvate transport: a historical perspective and future research directions", "journal": "Biochem. J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25748677/" } ], "x": 4451.333333333333, "y": 593.65, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9012597", "label": "GOT2 dimer transfers amino group from L-Cys to 2OG to form 3MPYR and Glu", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:17442" ], "source_url": "https://reactome.org/content/detail/R-HSA-9012597", "summary": "GOT2 dimer transfers amino group from L-Cys to 2OG to form 3MPYR and Glu is a Reactome release 97 curated reaction event. The source record connects L-Cys, 2OG to 3MPYR, L-Glu in mitochondrial matrix and cross-references RHEA:17442.", "equation": "L-Cys + 2OG → 3MPYR + L-Glu", "inputs": [ { "node_id": "R-ALL-379721", "reactome_id": "R-ALL-379721", "label": "L-Cys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113594", "reactome_id": "R-ALL-113594", "label": "2OG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9012592", "reactome_id": "R-ALL-9012592", "label": "3MPYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57678", "formula": "C3H3O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113552", "reactome_id": "R-ALL-113552", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-cysteine:2-oxoglutarate transaminase activity of GOT2 dimer [mitochondrial matrix]", "activity_id": "9012605", "ec_numbers": [ "2.6.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:9537447", "title": "Ethanol up-regulates fatty acid uptake and plasma membrane expression and export of mitochondrial aspartate aminotransferase in HepG2 cells", "journal": "Hepatology", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9537447/" } ], "x": 1530, "y": 2946.3333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9012721", "label": "MPST transfers sulfur atom from 3MPYR to HSO3- to form S2O3(2-) and PYR", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9012721", "summary": "MPST transfers sulfur atom from 3MPYR to HSO3- to form S2O3(2-) and PYR is a Reactome release 97 curated reaction event. The source record connects 3MPYR, HSO3- to S2O3(2-), PYR in mitochondrial matrix.", "equation": "3MPYR + HSO3- → S2O3(2-) + PYR", "inputs": [ { "node_id": "R-ALL-9012592", "reactome_id": "R-ALL-9012592", "label": "3MPYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57678", "formula": "C3H3O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9012724", "reactome_id": "R-ALL-9012724", "label": "HSO3-", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17137", "formula": "HO3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1614595", "reactome_id": "R-ALL-1614595", "label": "S2O3(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33542", "formula": "HO3S2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfurtransferase activity of MPST [mitochondrial matrix]", "activity_id": "9012723", "ec_numbers": [ "2.8.1" ], "accessions": [ "P25325" ] } ], "citations": [ { "id": "PMID:23698001", "title": "Structure and kinetic analysis of H2S production by human mercaptopyruvate sulfurtransferase", "journal": "J. Biol. Chem.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23698001/" } ], "x": 1251.3333333333333, "y": 3003.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9013198", "label": "TST transfers sulfur from S2O3(2-) to HCN to form HSCN", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:16881", "RHEA:16882" ], "source_url": "https://reactome.org/content/detail/R-HSA-9013198", "summary": "TST transfers sulfur from S2O3(2-) to HCN to form HSCN is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects S2O3(2-), HCN to HSCN, SO3(2-), H+ in mitochondrial matrix and cross-references RHEA:16881, RHEA:16882.", "equation": "S2O3(2-) + HCN → HSCN + SO3(2-) + 2 H+", "inputs": [ { "node_id": "R-ALL-1614595", "reactome_id": "R-ALL-1614595", "label": "S2O3(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33542", "formula": "HO3S2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9013199", "reactome_id": "R-ALL-9013199", "label": "HCN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18407", "formula": "CHN", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9013191", "reactome_id": "R-ALL-9013191", "label": "HSCN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18022", "formula": "CNS", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9013207", "reactome_id": "R-ALL-9013207", "label": "SO3(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17359", "formula": "O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "thiosulfate-cyanide sulfurtransferase activity of TST [mitochondrial matrix]", "activity_id": "1614667", "ec_numbers": [ "2.8.1.1" ], "accessions": [ "Q16762" ] } ], "citations": [ { "id": "PMID:18872649", "title": "Enzymatic conversion of cyanide to thiocyanate", "journal": "Am. J. Physiol.", "year": 1948, "url": "https://pubmed.ncbi.nlm.nih.gov/18872649/" }, { "id": "PMID:9070219", "title": "Cloning and expression of human liver rhodanese cDNA", "journal": "Biochem. Biophys. Res. Commun.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9070219/" }, { "id": "PMID:19625201", "title": "A partial exploration of the potential energy surfaces of SCN and HSCN: implications for the enzyme-mediated detoxification of cyanide", "journal": "J. Mol. Graph. Model.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19625201/" } ], "x": 1530, "y": 2850.777777777778, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9013471", "label": "MPST transfers sulfur from 3MPYR to HCN to form HSCN", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9013471", "summary": "MPST transfers sulfur from 3MPYR to HCN to form HSCN is a Reactome release 97 curated reaction event. The source record connects 3MPYR, HCN to HSCN, PYR in mitochondrial matrix.", "equation": "3MPYR + HCN → HSCN + PYR", "inputs": [ { "node_id": "R-ALL-9012592", "reactome_id": "R-ALL-9012592", "label": "3MPYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57678", "formula": "C3H3O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9013199", "reactome_id": "R-ALL-9013199", "label": "HCN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18407", "formula": "CHN", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9013191", "reactome_id": "R-ALL-9013191", "label": "HSCN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18022", "formula": "CNS", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfurtransferase activity of MPST [mitochondrial matrix]", "activity_id": "9012723", "ec_numbers": [ "2.8.1" ], "accessions": [ "P25325" ] } ], "citations": [ { "id": "PMID:18872649", "title": "Enzymatic conversion of cyanide to thiocyanate", "journal": "Am. J. Physiol.", "year": 1948, "url": "https://pubmed.ncbi.nlm.nih.gov/18872649/" }, { "id": "PMID:19625201", "title": "A partial exploration of the potential energy surfaces of SCN and HSCN: implications for the enzyme-mediated detoxification of cyanide", "journal": "J. Mol. Graph. Model.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19625201/" }, { "id": "PMID:28412453", "title": "In-vitro mercaptopyruvate sulfurtransferase species comparison in humans and common laboratory animals", "journal": "Toxicol. Lett.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28412453/" } ], "x": 1530, "y": 3003.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9013533", "label": "MPST transfers sulfur from sulfanegen to HCN to form HSCN", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9013533", "summary": "MPST transfers sulfur from sulfanegen to HCN to form HSCN is a Reactome release 97 curated reaction event. The source record connects sulfanegen, HCN to HSCN, PYR in mitochondrial matrix.", "equation": "sulfanegen + 2 HCN → 2 HSCN + 2 PYR", "inputs": [ { "node_id": "R-ALL-9013543", "reactome_id": "R-ALL-9013543", "label": "sulfanegen", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "ChemicalDrug", "currency": false }, { "node_id": "R-ALL-9013199", "reactome_id": "R-ALL-9013199", "label": "HCN", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:18407", "formula": "CHN", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9013191", "reactome_id": "R-ALL-9013191", "label": "HSCN", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:18022", "formula": "CNS", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfurtransferase activity of MPST [mitochondrial matrix]", "activity_id": "9012723", "ec_numbers": [ "2.8.1" ], "accessions": [ "P25325" ] } ], "citations": [ { "id": "PMID:12871026", "title": "Do antidotes for acute cyanide poisoning act on mercaptopyruvate sulfurtransferase to facilitate detoxification?", "journal": "Curr. Drug Targets Immune Endocr. Metabol. Disord.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12871026/" } ], "x": 1808.6666666666667, "y": 3003.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9014627", "label": "SDS dimers:PXLP convert L-Thr to 2OBUTA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "threonine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22109" ], "source_url": "https://reactome.org/content/detail/R-HSA-9014627", "summary": "SDS dimers:PXLP convert L-Thr to 2OBUTA is a Reactome release 97 curated reaction event. The source record connects L-Thr to NH4+, 2OBUTA in cytosol and cross-references RHEA:22109.", "equation": "L-Thr → NH4+ + 2OBUTA", "inputs": [ { "node_id": "R-ALL-351990", "reactome_id": "R-ALL-351990", "label": "L-Thr", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57926", "formula": "C4H9NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-936714", "reactome_id": "R-ALL-936714", "label": "2OBUTA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16763", "formula": "C4H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "threonine deaminase activity of PXLP-SDS dimers [cytosol]", "activity_id": "9014630", "ec_numbers": [ "4.3.1.19" ], "accessions": [] } ], "citations": [ { "id": "PMID:15689518", "title": "Crystal structure of the pyridoxal-5'-phosphate-dependent serine dehydratase from human liver", "journal": "Protein Sci.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15689518/" }, { "id": "PMID:18342636", "title": "A catalytic mechanism that explains a low catalytic activity of serine dehydratase like-1 from human cancer cells: crystal structure and site-directed mutagenesis studies", "journal": "Biochim. Biophys. Acta", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18342636/" } ], "x": 7740, "y": 2326.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9014641", "label": "HRSP12 deaminates 2-iminobutanoate to 2OBUTA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "threonine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:39976" ], "source_url": "https://reactome.org/content/detail/R-HSA-9014641", "summary": "HRSP12 deaminates 2-iminobutanoate to 2OBUTA is a Reactome release 97 curated reaction event. The source record connects 2-iminobutanoate, H2O to 2OBUTA, NH4+ in cytosol and cross-references RHEA:39976.", "equation": "2-iminobutanoate + H2O → 2OBUTA + NH4+", "inputs": [ { "node_id": "R-ALL-9929622", "reactome_id": "R-ALL-9929622", "label": "2-iminobutanoate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:76545", "formula": "C4H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-936714", "reactome_id": "R-ALL-936714", "label": "2OBUTA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16763", "formula": "C4H5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "2-iminobutanoate deaminase activity of HRSP12 [cytosol]", "activity_id": "9014617", "ec_numbers": null, "accessions": [ "P52758" ] } ], "citations": [ { "id": "PMID:22094463", "title": "Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22094463/" }, { "id": "PMID:23386433", "title": "RidA proteins prevent metabolic damage inflicted by PLP-dependent dehydratases in all domains of life", "journal": "MBio", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23386433/" }, { "id": "PMID:25975565", "title": "Genomic and experimental evidence for multiple metabolic functions in the RidA/YjgF/YER057c/UK114 (Rid) protein family", "journal": "BMC Genomics", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25975565/" }, { "id": "PMID:20306345", "title": "Cysteine S-conjugate β-lyases: important roles in the metabolism of naturally occurring sulfur and selenium-containing compounds, xenobiotics and anticancer agents", "journal": "Amino Acids", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/20306345/" }, { "id": "PMID:25620221", "title": "From microbiology to cancer biology: the Rid protein family prevents cellular damage caused by endogenously generated reactive nitrogen species", "journal": "Mol. Microbiol.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25620221/" } ], "x": 7461.333333333333, "y": 2326.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9014741", "label": "PXLP-K56-SRR dimer deaminates D-Ser", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "serine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:13978" ], "source_url": "https://reactome.org/content/detail/R-HSA-9014741", "summary": "PXLP-K56-SRR dimer deaminates D-Ser is a Reactome release 97 curated reaction event. The source record connects D-Ser to PYR, NH4+ in cytosol and cross-references RHEA:13978.", "equation": "D-Ser → PYR + NH4+", "inputs": [ { "node_id": "R-ALL-376186", "reactome_id": "R-ALL-376186", "label": "D-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35247", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "D-serine ammonia-lyase activity of PXLP-K56-SRR dimer [cytosol]", "activity_id": "9014746", "ec_numbers": [ "4.3.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:24795622", "title": "Serine racemase: a key player in apoptosis and necrosis", "journal": "Front Synaptic Neurosci", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24795622/" }, { "id": "PMID:11054547", "title": "Human serine racemase: moleular cloning, genomic organization and functional analysis", "journal": "Gene", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11054547/" }, { "id": "PMID:12393813", "title": "Cofactors of serine racemase that physiologically stimulate the synthesis of the N-methyl-D-aspartate (NMDA) receptor coagonist D-serine", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12393813/" }, { "id": "PMID:15536068", "title": "Serine racemase modulates intracellular D-serine levels through an alpha,beta-elimination activity", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15536068/" }, { "id": "PMID:20106978", "title": "The structure of mammalian serine racemase: evidence for conformational changes upon inhibitor binding", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20106978/" } ], "x": 311.33333333333337, "y": 2917.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9014766", "label": "PXLP-K56-SRR dimer isomerises L-Ser to D-Ser", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "serine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10981" ], "source_url": "https://reactome.org/content/detail/R-HSA-9014766", "summary": "PXLP-K56-SRR dimer isomerises L-Ser to D-Ser is a Reactome release 97 curated reaction event. The source record connects L-Ser to D-Ser in cytosol and cross-references RHEA:10981.", "equation": "L-Ser → D-Ser", "inputs": [ { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-376186", "reactome_id": "R-ALL-376186", "label": "D-Ser", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:35247", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "serine racemase activity of PXLP-K56-SRR dimer [cytosol]", "activity_id": "9014761", "ec_numbers": [ "5.1.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:24795622", "title": "Serine racemase: a key player in apoptosis and necrosis", "journal": "Front Synaptic Neurosci", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24795622/" }, { "id": "PMID:11054547", "title": "Human serine racemase: moleular cloning, genomic organization and functional analysis", "journal": "Gene", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11054547/" }, { "id": "PMID:12393813", "title": "Cofactors of serine racemase that physiologically stimulate the synthesis of the N-methyl-D-aspartate (NMDA) receptor coagonist D-serine", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12393813/" }, { "id": "PMID:15536068", "title": "Serine racemase modulates intracellular D-serine levels through an alpha,beta-elimination activity", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15536068/" }, { "id": "PMID:20106978", "title": "The structure of mammalian serine racemase: evidence for conformational changes upon inhibitor binding", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20106978/" }, { "id": "PMID:15193426", "title": "Characterization and localization of a human serine racemase", "journal": "Brain Res. Mol. Brain Res.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15193426/" } ], "x": 311.33333333333337, "y": 2994.1111111111113, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018858", "label": "ALOX5 oxidises 18(S)-HEPE to 5(S)-Hp-18(S)-HEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50205" ], "source_url": "https://reactome.org/content/detail/R-HSA-9018858", "summary": "ALOX5 oxidises 18(S)-HEPE to 5(S)-Hp-18(S)-HEPE is a Reactome release 97 curated reaction event. The source record connects 18(S)-HEPE, O2 to 5(S)-Hp-18(S)-HEPE in cytosol and cross-references RHEA:50205.", "equation": "18(S)-HEPE + O2 → 5(S)-Hp-18(S)-HEPE", "inputs": [ { "node_id": "R-ALL-9020474", "reactome_id": "R-ALL-9020474", "label": "18(S)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132083", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9020602", "reactome_id": "R-ALL-9020602", "label": "5(S)-Hp-18(S)-HEPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132091", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:17114001", "title": "Resolvin E2: identification and anti-inflammatory actions: pivotal role of human 5-lipoxygenase in resolvin E series biosynthesis", "journal": "Chem. Biol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17114001/" }, { "id": "PMID:22450811", "title": "Resolvin E2 formation and impact in inflammation resolution", "journal": "J. Immunol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22450811/" } ], "x": 5391.333333333333, "y": 2053.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018859", "label": "ALOX5 oxidises 5(S)-Hp-18(S)-HEPE to 5S,6S-epoxy-18(S)-HEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018859", "summary": "ALOX5 oxidises 5(S)-Hp-18(S)-HEPE to 5S,6S-epoxy-18(S)-HEPE is a Reactome release 97 curated reaction event. The source record connects 5(S)-Hp-18(S)-HEPE, O2 to 5S,6S-epoxy-18(S)-HEPE in cytosol.", "equation": "5(S)-Hp-18(S)-HEPE + O2 → 5S,6S-epoxy-18(S)-HEPE", "inputs": [ { "node_id": "R-ALL-9020602", "reactome_id": "R-ALL-9020602", "label": "5(S)-Hp-18(S)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132091", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9022655", "reactome_id": "R-ALL-9022655", "label": "5S,6S-epoxy-18(S)-HEPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747568", "formula": "C20H27O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:17114001", "title": "Resolvin E2: identification and anti-inflammatory actions: pivotal role of human 5-lipoxygenase in resolvin E series biosynthesis", "journal": "Chem. Biol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17114001/" }, { "id": "PMID:22450811", "title": "Resolvin E2 formation and impact in inflammation resolution", "journal": "J. Immunol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22450811/" } ], "x": 5670, "y": 2174.0833333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018862", "label": "LTA4H:Zn2+ hydrolyses 5S,6S-epoxy-18(S)-HEPE to 18(S)-RvE1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018862", "summary": "LTA4H:Zn2+ hydrolyses 5S,6S-epoxy-18(S)-HEPE to 18(S)-RvE1 is a Reactome release 97 curated reaction event. The source record connects 5S,6S-epoxy-18(S)-HEPE, H2O to 18(S)-RvE1 in cytosol.", "equation": "5S,6S-epoxy-18(S)-HEPE + H2O → 18(S)-RvE1", "inputs": [ { "node_id": "R-ALL-9022655", "reactome_id": "R-ALL-9022655", "label": "5S,6S-epoxy-18(S)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747568", "formula": "C20H27O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9022660", "reactome_id": "R-ALL-9022660", "label": "18(S)-RvE1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:136057", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "9022649", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:21206090", "title": "Pro-resolving actions and stereoselective biosynthesis of 18S E-series resolvins in human leukocytes and murine inflammation", "journal": "J. Clin. Invest.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21206090/" } ], "x": 5948.666666666667, "y": 2174.0833333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018863", "label": "ALOX5 oxidises 18(R)-HEPE to 5(S)-Hp-18(R)-HEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:51969" ], "source_url": "https://reactome.org/content/detail/R-HSA-9018863", "summary": "ALOX5 oxidises 18(R)-HEPE to 5(S)-Hp-18(R)-HEPE is a Reactome release 97 curated reaction event. The source record connects 18(R)-HEPE, O2 to 5(S)-Hp-18(R)-HEPE in cytosol and cross-references RHEA:51969.", "equation": "18(R)-HEPE + O2 → 5(S)-Hp-18(R)-HEPE", "inputs": [ { "node_id": "R-ALL-9023651", "reactome_id": "R-ALL-9023651", "label": "18(R)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90818", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9023702", "reactome_id": "R-ALL-9023702", "label": "5(S)-Hp-18(R)-HEPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132218", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:22450811", "title": "Resolvin E2 formation and impact in inflammation resolution", "journal": "J. Immunol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22450811/" }, { "id": "PMID:17114001", "title": "Resolvin E2: identification and anti-inflammatory actions: pivotal role of human 5-lipoxygenase in resolvin E series biosynthesis", "journal": "Chem. Biol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17114001/" } ], "x": 5670, "y": 2053.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018867", "label": "5-HEDH dehydrogenates 5(S)-Hp-18(S)-HpEPE to 18(S)-RvE2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:52025" ], "source_url": "https://reactome.org/content/detail/R-HSA-9018867", "summary": "5-HEDH dehydrogenates 5(S)-Hp-18(S)-HpEPE to 18(S)-RvE2 is a Reactome release 97 curated reaction event. The source record connects 5(S)-Hp-18(S)-HEPE, hydrogen donor to 18(S)-RvE2, hydrogen acceptor, H2O in cytosol and cross-references RHEA:52025.", "equation": "5(S)-Hp-18(S)-HEPE + hydrogen donor → 18(S)-RvE2 + hydrogen acceptor + H2O", "inputs": [ { "node_id": "R-ALL-9020602", "reactome_id": "R-ALL-9020602", "label": "5(S)-Hp-18(S)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132091", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1131953", "reactome_id": "R-ALL-1131953", "label": "hydrogen donor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17499", "formula": "H2A", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9022650", "reactome_id": "R-ALL-9022650", "label": "18(S)-RvE2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:136056", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1130886", "reactome_id": "R-ALL-1130886", "label": "hydrogen acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13193", "formula": "A", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NADPH dehydrogenase activity of 5-HEDH [cytosol]", "activity_id": "9022666", "ec_numbers": [ "1.6.99.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:17114001", "title": "Resolvin E2: identification and anti-inflammatory actions: pivotal role of human 5-lipoxygenase in resolvin E series biosynthesis", "journal": "Chem. Biol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17114001/" }, { "id": "PMID:22450811", "title": "Resolvin E2 formation and impact in inflammation resolution", "journal": "J. Immunol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22450811/" }, { "id": "PMID:19164464", "title": "Substrate selectivity of 5-hydroxyeicosanoid dehydrogenase and its inhibition by 5-hydroxy-Delta6-long-chain fatty acids", "journal": "J. Pharmacol. Exp. Ther.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19164464/" } ], "x": 5391.333333333333, "y": 2025.892857142857, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018868", "label": "GPX4-2 reduces 18(S)-HpEPE to 18(S)-HEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018868", "summary": "GPX4-2 reduces 18(S)-HpEPE to 18(S)-HEPE is a Reactome release 97 curated reaction event. The source record connects 18(S)-HpEPE, GSH to 18(S)-HEPE, GSSG, H2O in cytosol.", "equation": "18(S)-HpEPE + 2 GSH → 18(S)-HEPE + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-9020583", "reactome_id": "R-ALL-9020583", "label": "18(S)-HpEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747601", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9020474", "reactome_id": "R-ALL-9020474", "label": "18(S)-HEPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132083", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phospholipid-hydroperoxide glutathione peroxidase activity of GPX4-2 [cytosol]", "activity_id": "9020570", "ec_numbers": [ "1.11.1.12" ], "accessions": [ "P36969" ] } ], "citations": [ { "id": "PMID:24170573", "title": "Expression and characterization of recombinant human phospholipid hydroperoxide glutathione peroxidase", "journal": "IUBMB Life", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24170573/" }, { "id": "PMID:8605014", "title": "Expression of human phospholipid hydroperoxide glutathione peroxidase gene for protection of host cells from lipid hydroperoxide-mediated injury", "journal": "Biochem. Biophys. Res. Commun.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8605014/" } ], "x": 5948.666666666667, "y": 2090.7261904761904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018874", "label": "CYP monooxygenates EPA to 18(S)-HpEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018874", "summary": "CYP monooxygenates EPA to 18(S)-HpEPE is a Reactome release 97 curated reaction event. The source record connects EPA, NADPH, O2 to 18(S)-HpEPE, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "EPA + NADPH + O2 + H+ → 18(S)-HpEPE + NADP+", "inputs": [ { "node_id": "R-ALL-2026977", "reactome_id": "R-ALL-2026977", "label": "EPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58562", "formula": "C20H29O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9020583", "reactome_id": "R-ALL-9020583", "label": "18(S)-HpEPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747601", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP [endoplasmic reticulum membrane]", "activity_id": "9029002", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:20732876", "title": "Arachidonic acid-metabolizing cytochrome P450 enzymes are targets of {omega}-3 fatty acids", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20732876/" } ], "x": 5948.666666666667, "y": 2081.464285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018877", "label": "LTA4H:Zn2+ hydrolyses 5S,6S-epoxy-18(R)-HEPE to 18(R)-RvE1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018877", "summary": "LTA4H:Zn2+ hydrolyses 5S,6S-epoxy-18(R)-HEPE to 18(R)-RvE1 is a Reactome release 97 curated reaction event. The source record connects 5S,6S-epoxy-18(R)-HEPE, H2O to 18(R)-RvE1 in cytosol.", "equation": "5S,6S-epoxy-18(R)-HEPE + H2O → 18(R)-RvE1", "inputs": [ { "node_id": "R-ALL-9023708", "reactome_id": "R-ALL-9023708", "label": "5S,6S-epoxy-18(R)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747604", "formula": "C20H27O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9023712", "reactome_id": "R-ALL-9023712", "label": "18(R)-RvE1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:91000", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "9022649", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:2995393", "title": "Enzymatic hydration of leukotriene A4. Purification and characterization of a novel epoxide hydrolase from human erythrocytes", "journal": "J Biol Chem", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/2995393/" } ], "x": 5391.333333333333, "y": 2183.345238095238, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018880", "label": "Ac-PTGS2 dimer oxidises EPA to 18(R)-HpEPE or 18(S)-HpEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018880", "summary": "Ac-PTGS2 dimer oxidises EPA to 18(R)-HpEPE or 18(S)-HpEPE is a Reactome release 97 curated reaction event. The source record connects EPA, O2 to 18(S)-HpEPE, 18(R)-HpEPE in cytosol · endoplasmic reticulum membrane.", "equation": "EPA + O2 → 18(S)-HpEPE, 18(R)-HpEPE", "inputs": [ { "node_id": "R-ALL-2026977", "reactome_id": "R-ALL-2026977", "label": "EPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58562", "formula": "C20H29O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9022921", "reactome_id": "R-ALL-9022921", "label": "18(S)-HpEPE, 18(R)-HpEPE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen of Ac-PTGS2 dimer [endoplasmic reticulum membrane]", "activity_id": "9022922", "ec_numbers": [ "1.13.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:11034610", "title": "Novel functional sets of lipid-derived mediators with antiinflammatory actions generated from omega-3 fatty acids via cyclooxygenase 2-nonsteroidal antiinflammatory drugs and transcellular processing", "journal": "J. Exp. Med.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11034610/" }, { "id": "PMID:21206090", "title": "Pro-resolving actions and stereoselective biosynthesis of 18S E-series resolvins in human leukocytes and murine inflammation", "journal": "J. Clin. Invest.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21206090/" } ], "x": 5391.333333333333, "y": 2275.964285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018894", "label": "ALOX5 oxidises 5(S)-Hp-18(R)-HEPE to 5S,6S-epoxy-18(R)-HEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018894", "summary": "ALOX5 oxidises 5(S)-Hp-18(R)-HEPE to 5S,6S-epoxy-18(R)-HEPE is a Reactome release 97 curated reaction event. The source record connects 5(S)-Hp-18(R)-HEPE, O2 to 5S,6S-epoxy-18(R)-HEPE in cytosol.", "equation": "5(S)-Hp-18(R)-HEPE + O2 → 5S,6S-epoxy-18(R)-HEPE", "inputs": [ { "node_id": "R-ALL-9023702", "reactome_id": "R-ALL-9023702", "label": "5(S)-Hp-18(R)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132218", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9023708", "reactome_id": "R-ALL-9023708", "label": "5S,6S-epoxy-18(R)-HEPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747604", "formula": "C20H27O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:17114001", "title": "Resolvin E2: identification and anti-inflammatory actions: pivotal role of human 5-lipoxygenase in resolvin E series biosynthesis", "journal": "Chem. Biol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17114001/" }, { "id": "PMID:22450811", "title": "Resolvin E2 formation and impact in inflammation resolution", "journal": "J. Immunol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22450811/" } ], "x": 5670, "y": 2183.345238095238, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018895", "label": "GPX4-2 reduces 18(R)-HpEPE to 18(R)-HEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018895", "summary": "GPX4-2 reduces 18(R)-HpEPE to 18(R)-HEPE is a Reactome release 97 curated reaction event. The source record connects 18(R)-HpEPE, GSH to 18(R)-HEPE, GSSG, H2O in cytosol.", "equation": "18(R)-HpEPE + 2 GSH → 18(R)-HEPE + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-9023653", "reactome_id": "R-ALL-9023653", "label": "18(R)-HpEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747570", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9023651", "reactome_id": "R-ALL-9023651", "label": "18(R)-HEPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90818", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phospholipid-hydroperoxide glutathione peroxidase activity of GPX4-2 [cytosol]", "activity_id": "9020570", "ec_numbers": [ "1.11.1.12" ], "accessions": [ "P36969" ] } ], "citations": [ { "id": "PMID:24170573", "title": "Expression and characterization of recombinant human phospholipid hydroperoxide glutathione peroxidase", "journal": "IUBMB Life", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24170573/" }, { "id": "PMID:8605014", "title": "Expression of human phospholipid hydroperoxide glutathione peroxidase gene for protection of host cells from lipid hydroperoxide-mediated injury", "journal": "Biochem. Biophys. Res. Commun.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8605014/" } ], "x": 5391.333333333333, "y": 2099.9880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018901", "label": "5-HEDH dehydrogenates 5(S)-Hp-18(R)-HEPE to 18(R)-RvE2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:52021" ], "source_url": "https://reactome.org/content/detail/R-HSA-9018901", "summary": "5-HEDH dehydrogenates 5(S)-Hp-18(R)-HEPE to 18(R)-RvE2 is a Reactome release 97 curated reaction event. The source record connects 5(S)-Hp-18(R)-HEPE, hydrogen donor to 18(R)-RvE2, hydrogen acceptor, H2O in cytosol and cross-references RHEA:52021.", "equation": "5(S)-Hp-18(R)-HEPE + hydrogen donor → 18(R)-RvE2 + hydrogen acceptor + H2O", "inputs": [ { "node_id": "R-ALL-9023702", "reactome_id": "R-ALL-9023702", "label": "5(S)-Hp-18(R)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132218", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1131953", "reactome_id": "R-ALL-1131953", "label": "hydrogen donor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17499", "formula": "H2A", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9023700", "reactome_id": "R-ALL-9023700", "label": "18(R)-RvE2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133368", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1130886", "reactome_id": "R-ALL-1130886", "label": "hydrogen acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13193", "formula": "A", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NADPH dehydrogenase activity of 5-HEDH [cytosol]", "activity_id": "9022666", "ec_numbers": [ "1.6.99.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:22450811", "title": "Resolvin E2 formation and impact in inflammation resolution", "journal": "J. Immunol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22450811/" }, { "id": "PMID:17114001", "title": "Resolvin E2: identification and anti-inflammatory actions: pivotal role of human 5-lipoxygenase in resolvin E series biosynthesis", "journal": "Chem. Biol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17114001/" }, { "id": "PMID:19164464", "title": "Substrate selectivity of 5-hydroxyeicosanoid dehydrogenase and its inhibition by 5-hydroxy-Delta6-long-chain fatty acids", "journal": "J. Pharmacol. Exp. Ther.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19164464/" } ], "x": 5670, "y": 2025.892857142857, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9018907", "label": "ALOX15 oxidises 18(R)-HEPE to 18(R)-RvE3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9018907", "summary": "ALOX15 oxidises 18(R)-HEPE to 18(R)-RvE3 is a Reactome release 97 curated reaction event. The source record connects 18(R)-HEPE, O2 to (18R)-resolvin E3 in cytosol.", "equation": "18(R)-HEPE + O2 → (18R)-resolvin E3", "inputs": [ { "node_id": "R-ALL-9023651", "reactome_id": "R-ALL-9023651", "label": "18(R)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90818", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9023644", "reactome_id": "R-ALL-9023644", "label": "(18R)-resolvin E3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747569", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15 [cytosol]", "activity_id": "9022673", "ec_numbers": [ "1.13.11.33" ], "accessions": [ "P16050" ] } ], "citations": [ { "id": "PMID:22275352", "title": "Identification and structure determination of novel anti-inflammatory mediator resolvin E3, 17,18-dihydroxyeicosapentaenoic acid", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22275352/" }, { "id": "PMID:23293324", "title": "Stereochemical assignment and anti-inflammatory properties of the omega-3 lipid mediator resolvin E3", "journal": "J. Biochem.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23293324/" } ], "x": 5948.666666666667, "y": 2183.345238095238, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020249", "label": "Hydroperoxy reductase reduces 4(S)-Hp-17(S)-HDHA to RvD6", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020249", "summary": "Hydroperoxy reductase reduces 4(S)-Hp-17(S)-HDHA to RvD6 is a Reactome release 97 curated reaction event. The source record connects 4(S)-Hp-17(S)-HDHA, reduced acceptor to resolvin D6, oxidised acceptor in cytosol.", "equation": "4(S)-Hp-17(S)-HDHA + reduced acceptor → resolvin D6 + oxidised acceptor", "inputs": [ { "node_id": "R-ALL-9024797", "reactome_id": "R-ALL-9024797", "label": "4(S)-Hp-17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747595", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359016", "reactome_id": "R-ALL-5359016", "label": "reduced acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024803", "reactome_id": "R-ALL-9024803", "label": "resolvin D6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747593", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9024637", "reactome_id": "R-ALL-9024637", "label": "oxidised acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of Hydroperoxy reductase [cytosol]", "activity_id": "9024806", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5670, "y": 2275.964285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020251", "label": "ALOX5 oxidises 17(R)-HDHA to 7(S)-Hp-17(R)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020251", "summary": "ALOX5 oxidises 17(R)-HDHA to 7(S)-Hp-17(R)-HDHA is a Reactome release 97 curated reaction event. The source record connects 17(R)-HDHA, O2 to 7(S)-Hp-17(R)-HDHA in cytosol.", "equation": "17(R)-HDHA + O2 → 7(S)-Hp-17(R)-HDHA", "inputs": [ { "node_id": "R-ALL-9024463", "reactome_id": "R-ALL-9024463", "label": "17(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90814", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024479", "reactome_id": "R-ALL-9024479", "label": "7(S)-Hp-17(R)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747574", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5391.333333333333, "y": 2192.6071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020252", "label": "LTA4H:Zn2+ hydrolyses 7S(8)-epoxy-17(R)-HDHA to AT-RvD1 or AT-RvD2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020252", "summary": "LTA4H:Zn2+ hydrolyses 7S(8)-epoxy-17(R)-HDHA to AT-RvD1 or AT-RvD2 is a Reactome release 97 curated reaction event. The source record connects 7S(8)-epoxy-17(R)-HDHA, H2O to AT-RvD1, AT-RvD2 in cytosol.", "equation": "7S(8)-epoxy-17(R)-HDHA + H2O → AT-RvD1, AT-RvD2", "inputs": [ { "node_id": "R-ALL-9024602", "reactome_id": "R-ALL-9024602", "label": "7S(8)-epoxy-17(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747576", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024574", "reactome_id": "R-ALL-9024574", "label": "AT-RvD1, AT-RvD2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "9022649", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:17244615", "title": "Resolvin D1 and its aspirin-triggered 17R epimer. Stereochemical assignments, anti-inflammatory properties, and enzymatic inactivation", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17244615/" }, { "id": "PMID:19865173", "title": "Resolvin D2 is a potent regulator of leukocytes and controls microbial sepsis", "journal": "Nature", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19865173/" }, { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5948.666666666667, "y": 2275.964285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020253", "label": "LTA4H:Zn2+ hydrolyses 4S(5)-epoxy-17(S)-HDHA to RvD3 or RvD4", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020253", "summary": "LTA4H:Zn2+ hydrolyses 4S(5)-epoxy-17(S)-HDHA to RvD3 or RvD4 is a Reactome release 97 curated reaction event. The source record connects 4S(5)-epoxy-17(S)-HDHA, H2O to RvD3, RvD4 in cytosol.", "equation": "4S(5)-epoxy-17(S)-HDHA + H2O → RvD3, RvD4", "inputs": [ { "node_id": "R-ALL-9024781", "reactome_id": "R-ALL-9024781", "label": "4S(5)-epoxy-17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747582", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024809", "reactome_id": "R-ALL-9024809", "label": "RvD3, RvD4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "9022649", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:23438748", "title": "Resolvin D3 and aspirin-triggered resolvin D3 are potent immunoresolvents", "journal": "Chem. Biol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23438748/" }, { "id": "PMID:28980694", "title": "Stereocontrolled synthesis and investigation of the biosynthetic transformations of 16(S),17(S)-epoxy-PDn-3 DPA", "journal": "Org. Biomol. Chem.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28980694/" }, { "id": "PMID:26743932", "title": "Resolvin D4 stereoassignment and its novel actions in host protection and bacterial clearance", "journal": "Sci Rep", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26743932/" } ], "x": 5391.333333333333, "y": 2285.2261904761904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020255", "label": "ALOX5 dehydrogenates 7(S)-Hp-17(S)-HDHA to 7S(8)-epoxy-17S-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020255", "summary": "ALOX5 dehydrogenates 7(S)-Hp-17(S)-HDHA to 7S(8)-epoxy-17S-HDHA is a Reactome release 97 curated reaction event. The source record connects 7(S)-Hp-17(S)-HDHA, NADP+ to 7S(8)-epoxy-17(S)-HDHA, NADPH in cytosol.", "equation": "7(S)-Hp-17(S)-HDHA + NADP+ → 7S(8)-epoxy-17(S)-HDHA + NADPH", "inputs": [ { "node_id": "R-ALL-9024765", "reactome_id": "R-ALL-9024765", "label": "7(S)-Hp-17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747594", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024768", "reactome_id": "R-ALL-9024768", "label": "7S(8)-epoxy-17(S)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747581", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5948.666666666667, "y": 2109.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020256", "label": "ALOX5 dehydrogenates 7(S)-Hp-17R-HDHA to 7S(8)-epoxy-17R-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020256", "summary": "ALOX5 dehydrogenates 7(S)-Hp-17R-HDHA to 7S(8)-epoxy-17R-HDHA is a Reactome release 97 curated reaction event. The source record connects 7(S)-Hp-17(R)-HDHA, NADP+ to 7S(8)-epoxy-17(R)-HDHA, NADPH in cytosol.", "equation": "7(S)-Hp-17(R)-HDHA + NADP+ → 7S(8)-epoxy-17(R)-HDHA + NADPH", "inputs": [ { "node_id": "R-ALL-9024479", "reactome_id": "R-ALL-9024479", "label": "7(S)-Hp-17(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747574", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024602", "reactome_id": "R-ALL-9024602", "label": "7S(8)-epoxy-17(R)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747576", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5391.333333333333, "y": 2118.5119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020257", "label": "LTA4H:Zn2+ hydrolyses 17R(16)-epoxy-DHA to AT-(N)PD1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020257", "summary": "LTA4H:Zn2+ hydrolyses 17R(16)-epoxy-DHA to AT-(N)PD1 is a Reactome release 97 curated reaction event. The source record connects 17R(16)-epoxy-DHA, H2O to AT-(N)PD1 in cytosol.", "equation": "17R(16)-epoxy-DHA + H2O → AT-(N)PD1", "inputs": [ { "node_id": "R-ALL-9024893", "reactome_id": "R-ALL-9024893", "label": "17R(16)-epoxy-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140346", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024879", "reactome_id": "R-ALL-9024879", "label": "AT-(N)PD1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140208", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "9022649", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:21867913", "title": "Novel proresolving aspirin-triggered DHA pathway", "journal": "Chem. Biol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21867913/" } ], "x": 5670, "y": 2192.6071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020258", "label": "LTA4H:Zn2+ hydrolyses 7S(8)-epoxy-17(S)-HDHA to RvD1 or RvD2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020258", "summary": "LTA4H:Zn2+ hydrolyses 7S(8)-epoxy-17(S)-HDHA to RvD1 or RvD2 is a Reactome release 97 curated reaction event. The source record connects 7S(8)-epoxy-17(S)-HDHA, H2O to RvD1, RvD2 in cytosol.", "equation": "7S(8)-epoxy-17(S)-HDHA + H2O → RvD1, RvD2", "inputs": [ { "node_id": "R-ALL-9024768", "reactome_id": "R-ALL-9024768", "label": "7S(8)-epoxy-17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747581", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024759", "reactome_id": "R-ALL-9024759", "label": "RvD1, RvD2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "9022649", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:19865173", "title": "Resolvin D2 is a potent regulator of leukocytes and controls microbial sepsis", "journal": "Nature", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19865173/" }, { "id": "PMID:17244615", "title": "Resolvin D1 and its aspirin-triggered 17R epimer. Stereochemical assignments, anti-inflammatory properties, and enzymatic inactivation", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17244615/" }, { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5670, "y": 2285.2261904761904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020259", "label": "ALOX5 oxidises 17(R)-HDHA to 4(S)-Hp-17(R)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020259", "summary": "ALOX5 oxidises 17(R)-HDHA to 4(S)-Hp-17(R)-HDHA is a Reactome release 97 curated reaction event. The source record connects 17(R)-HDHA, O2 to 4(S)-Hp-17(R)-HDHA in cytosol.", "equation": "17(R)-HDHA + O2 → 4(S)-Hp-17(R)-HDHA", "inputs": [ { "node_id": "R-ALL-9024463", "reactome_id": "R-ALL-9024463", "label": "17(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90814", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024576", "reactome_id": "R-ALL-9024576", "label": "4(S)-Hp-17(R)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747573", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5948.666666666667, "y": 2192.6071428571427, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020260", "label": "Hydroperoxy reductase reduces 7(S)-Hp-17(S)-HDHA to RvD5", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020260", "summary": "Hydroperoxy reductase reduces 7(S)-Hp-17(S)-HDHA to RvD5 is a Reactome release 97 curated reaction event. The source record connects 7(S)-Hp-17(S)-HDHA, reduced acceptor to resolvin D5, oxidised acceptor in cytosol.", "equation": "7(S)-Hp-17(S)-HDHA + reduced acceptor → resolvin D5 + oxidised acceptor", "inputs": [ { "node_id": "R-ALL-9024765", "reactome_id": "R-ALL-9024765", "label": "7(S)-Hp-17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747594", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359016", "reactome_id": "R-ALL-5359016", "label": "reduced acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024795", "reactome_id": "R-ALL-9024795", "label": "resolvin D5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140352", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9024637", "reactome_id": "R-ALL-9024637", "label": "oxidised acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of Hydroperoxy reductase [cytosol]", "activity_id": "9024806", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5948.666666666667, "y": 2285.2261904761904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020261", "label": "Ac-PTGS2 dimer oxidises DHA to 17(R)-Hp-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020261", "summary": "Ac-PTGS2 dimer oxidises DHA to 17(R)-Hp-DHA is a Reactome release 97 curated reaction event. The source record connects DHA, O2 to 17(R)-Hp-DHA in cytosol.", "equation": "DHA + O2 → 17(R)-Hp-DHA", "inputs": [ { "node_id": "R-ALL-9024011", "reactome_id": "R-ALL-9024011", "label": "DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024522", "reactome_id": "R-ALL-9024522", "label": "17(R)-Hp-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747572", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of Ac-PTGS2 dimer [endoplasmic reticulum membrane]", "activity_id": "2161919", "ec_numbers": [ "1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" }, { "id": "PMID:17244615", "title": "Resolvin D1 and its aspirin-triggered 17R epimer. Stereochemical assignments, anti-inflammatory properties, and enzymatic inactivation", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17244615/" } ], "x": 5391.333333333333, "y": 2201.8690476190477, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020262", "label": "ALOX15 dehydrogenates 17(R)-Hp-DHA to 17R(16)-epoxy-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020262", "summary": "ALOX15 dehydrogenates 17(R)-Hp-DHA to 17R(16)-epoxy-DHA is a Reactome release 97 curated reaction event. The source record connects 17(R)-Hp-DHA, NADP+ to 17R(16)-epoxy-DHA, NADPH in cytosol.", "equation": "17(R)-Hp-DHA + NADP+ → 17R(16)-epoxy-DHA + NADPH", "inputs": [ { "node_id": "R-ALL-9024522", "reactome_id": "R-ALL-9024522", "label": "17(R)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747572", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024893", "reactome_id": "R-ALL-9024893", "label": "17R(16)-epoxy-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140346", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15 [cytosol]", "activity_id": "9022673", "ec_numbers": [ "1.13.11.33" ], "accessions": [ "P16050" ] } ], "citations": [ { "id": "PMID:21867913", "title": "Novel proresolving aspirin-triggered DHA pathway", "journal": "Chem. Biol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21867913/" } ], "x": 5670, "y": 2118.5119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020264", "label": "ALOX5 oxidises 17(S)-HDHA to 4(S)-Hp-17(S)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020264", "summary": "ALOX5 oxidises 17(S)-HDHA to 4(S)-Hp-17(S)-HDHA is a Reactome release 97 curated reaction event. The source record connects 17(S)-HDHA, O2 to 4(S)-Hp-17(S)-HDHA in cytosol.", "equation": "17(S)-HDHA + O2 → 4(S)-Hp-17(S)-HDHA", "inputs": [ { "node_id": "R-ALL-9024815", "reactome_id": "R-ALL-9024815", "label": "17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195403", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024797", "reactome_id": "R-ALL-9024797", "label": "4(S)-Hp-17(S)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747595", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5670, "y": 2201.8690476190477, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020270", "label": "LTA4H:Zn2+ hydrolyses 4S(5)-epoxy-17(R)-HDHA to AT-RvD3 or AT-RvD4", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020270", "summary": "LTA4H:Zn2+ hydrolyses 4S(5)-epoxy-17(R)-HDHA to AT-RvD3 or AT-RvD4 is a Reactome release 97 curated reaction event. The source record connects 4S(5)-epoxy-17(R)-HDHA, H2O to AT-RvD3, AT-RvD4 in cytosol.", "equation": "4S(5)-epoxy-17(R)-HDHA + H2O → AT-RvD3, AT-RvD4", "inputs": [ { "node_id": "R-ALL-9024577", "reactome_id": "R-ALL-9024577", "label": "4S(5)-epoxy-17(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747575", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024570", "reactome_id": "R-ALL-9024570", "label": "AT-RvD3, AT-RvD4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "9022649", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" }, { "id": "PMID:23438748", "title": "Resolvin D3 and aspirin-triggered resolvin D3 are potent immunoresolvents", "journal": "Chem. Biol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23438748/" }, { "id": "PMID:28980694", "title": "Stereocontrolled synthesis and investigation of the biosynthetic transformations of 16(S),17(S)-epoxy-PDn-3 DPA", "journal": "Org. Biomol. Chem.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28980694/" }, { "id": "PMID:26743932", "title": "Resolvin D4 stereoassignment and its novel actions in host protection and bacterial clearance", "journal": "Sci Rep", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26743932/" } ], "x": 5391.333333333333, "y": 2294.4880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020271", "label": "GPX4-2 reduces 17(R)-Hp-DHA to 17(R)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020271", "summary": "GPX4-2 reduces 17(R)-Hp-DHA to 17(R)-HDHA is a Reactome release 97 curated reaction event. The source record connects 17(R)-Hp-DHA, GSH to 17(R)-HDHA, GSSG, H2O in cytosol.", "equation": "17(R)-Hp-DHA + 2 GSH → 17(R)-HDHA + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-9024522", "reactome_id": "R-ALL-9024522", "label": "17(R)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747572", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024463", "reactome_id": "R-ALL-9024463", "label": "17(R)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90814", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phospholipid-hydroperoxide glutathione peroxidase activity of GPX4-2 [cytosol]", "activity_id": "9020570", "ec_numbers": [ "1.11.1.12" ], "accessions": [ "P36969" ] } ], "citations": [ { "id": "PMID:24170573", "title": "Expression and characterization of recombinant human phospholipid hydroperoxide glutathione peroxidase", "journal": "IUBMB Life", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24170573/" }, { "id": "PMID:8605014", "title": "Expression of human phospholipid hydroperoxide glutathione peroxidase gene for protection of host cells from lipid hydroperoxide-mediated injury", "journal": "Biochem. Biophys. Res. Commun.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8605014/" } ], "x": 5670, "y": 2099.9880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020273", "label": "GPX4-2 reduces 17(S)-Hp-DHA to 17(S)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:76716" ], "source_url": "https://reactome.org/content/detail/R-HSA-9020273", "summary": "GPX4-2 reduces 17(S)-Hp-DHA to 17(S)-HDHA is a Reactome release 97 curated reaction event. The source record connects 17(S)-Hp-DHA, GSH to 17(S)-HDHA, GSSG, H2O in cytosol and cross-references RHEA:76716.", "equation": "17(S)-Hp-DHA + 2 GSH → 17(S)-HDHA + GSSG + H2O", "inputs": [ { "node_id": "R-ALL-9024012", "reactome_id": "R-ALL-9024012", "label": "17(S)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133795", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024815", "reactome_id": "R-ALL-9024815", "label": "17(S)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195403", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111745", "reactome_id": "R-ALL-111745", "label": "GSSG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58297", "formula": "C20H30N6O12S2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phospholipid-hydroperoxide glutathione peroxidase activity of GPX4-2 [cytosol]", "activity_id": "9020570", "ec_numbers": [ "1.11.1.12" ], "accessions": [ "P36969" ] } ], "citations": [ { "id": "PMID:24170573", "title": "Expression and characterization of recombinant human phospholipid hydroperoxide glutathione peroxidase", "journal": "IUBMB Life", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24170573/" }, { "id": "PMID:8605014", "title": "Expression of human phospholipid hydroperoxide glutathione peroxidase gene for protection of host cells from lipid hydroperoxide-mediated injury", "journal": "Biochem. Biophys. Res. Commun.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8605014/" } ], "x": 5948.666666666667, "y": 2025.892857142857, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020274", "label": "ALOX12:Fe2+ oxidises DHA to 14(S)-Hp-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020274", "summary": "ALOX12:Fe2+ oxidises DHA to 14(S)-Hp-DHA is a Reactome release 97 curated reaction event. The source record connects DHA, O2 to 14(S)-Hp-DHA in cytosol.", "equation": "DHA + O2 → 14(S)-Hp-DHA", "inputs": [ { "node_id": "R-ALL-9024011", "reactome_id": "R-ALL-9024011", "label": "DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9025012", "reactome_id": "R-ALL-9025012", "label": "14(S)-Hp-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140279", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen of ALOX12:Fe2+ [cytosol]", "activity_id": "9024992", "ec_numbers": [ "1.13.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:19103881", "title": "Maresins: novel macrophage mediators with potent antiinflammatory and proresolving actions", "journal": "J. Exp. Med.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19103881/" }, { "id": "PMID:25036362", "title": "Maresin biosynthesis and identification of maresin 2, a new anti-inflammatory and pro-resolving mediator from human macrophages", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25036362/" } ], "x": 5948.666666666667, "y": 2201.8690476190477, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020275", "label": "ALOX15 oxidises DHA to 17(S)-Hp-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50841" ], "source_url": "https://reactome.org/content/detail/R-HSA-9020275", "summary": "ALOX15 oxidises DHA to 17(S)-Hp-DHA is a Reactome release 97 curated reaction event. The source record connects DHA, O2 to 17(S)-Hp-DHA in cytosol and cross-references RHEA:50841.", "equation": "DHA + O2 → 17(S)-Hp-DHA", "inputs": [ { "node_id": "R-ALL-9024011", "reactome_id": "R-ALL-9024011", "label": "DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024012", "reactome_id": "R-ALL-9024012", "label": "17(S)-Hp-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133795", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15 [cytosol]", "activity_id": "9022673", "ec_numbers": [ "1.13.11.33" ], "accessions": [ "P16050" ] } ], "citations": [ { "id": "PMID:12590139", "title": "Novel docosatrienes and 17S-resolvins generated from docosahexaenoic acid in murine brain, human blood, and glial cells. Autacoids in anti-inflammation", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12590139/" }, { "id": "PMID:2147774", "title": "Formation of 15-lipoxygenase product from docosahexaenoic acid (22:6w3) by human platelets", "journal": "Prostaglandins", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2147774/" } ], "x": 5948.666666666667, "y": 2053.6785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020277", "label": "ALOX5 dehydrogenates 4(S)-Hp-17(S)-HDHA to 4S(5)-epoxy-17(S)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020277", "summary": "ALOX5 dehydrogenates 4(S)-Hp-17(S)-HDHA to 4S(5)-epoxy-17(S)-HDHA is a Reactome release 97 curated reaction event. The source record connects 4(S)-Hp-17(S)-HDHA, NADP+ to 4S(5)-epoxy-17(S)-HDHA, NADPH in cytosol.", "equation": "4(S)-Hp-17(S)-HDHA + NADP+ → 4S(5)-epoxy-17(S)-HDHA + NADPH", "inputs": [ { "node_id": "R-ALL-9024797", "reactome_id": "R-ALL-9024797", "label": "4(S)-Hp-17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747595", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024781", "reactome_id": "R-ALL-9024781", "label": "4S(5)-epoxy-17(S)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747582", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5948.666666666667, "y": 2118.5119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020278", "label": "ALOX5 dehydrogenates 4(S)-Hp-17(R)-HDHA to 4S(5)-epoxy-17(R)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020278", "summary": "ALOX5 dehydrogenates 4(S)-Hp-17(R)-HDHA to 4S(5)-epoxy-17(R)-HDHA is a Reactome release 97 curated reaction event. The source record connects 4(S)-Hp-17(R)-HDHA, NADP+ to 4S(5)-epoxy-17(R)-HDHA, NADPH in cytosol.", "equation": "4(S)-Hp-17(R)-HDHA + NADP+ → 4S(5)-epoxy-17(R)-HDHA + NADPH", "inputs": [ { "node_id": "R-ALL-9024576", "reactome_id": "R-ALL-9024576", "label": "4(S)-Hp-17(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747573", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024577", "reactome_id": "R-ALL-9024577", "label": "4S(5)-epoxy-17(R)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747575", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5391.333333333333, "y": 2127.7738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020282", "label": "ALOX5 oxidises 17(S)-HDHA to 7(S)-Hp-17(S)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020282", "summary": "ALOX5 oxidises 17(S)-HDHA to 7(S)-Hp-17(S)-HDHA is a Reactome release 97 curated reaction event. The source record connects 17(S)-HDHA, O2 to 7(S)-Hp-17(S)-HDHA in cytosol.", "equation": "17(S)-HDHA + O2 → 7(S)-Hp-17(S)-HDHA", "inputs": [ { "node_id": "R-ALL-9024815", "reactome_id": "R-ALL-9024815", "label": "17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195403", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024765", "reactome_id": "R-ALL-9024765", "label": "7(S)-Hp-17(S)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747594", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5391.333333333333, "y": 2211.1309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9020610", "label": "ALOX15 oxidises 18(S)-HEPE to 18(S)-RvE3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9020610", "summary": "ALOX15 oxidises 18(S)-HEPE to 18(S)-RvE3 is a Reactome release 97 curated reaction event. The source record connects 18(S)-HEPE, O2 to 18(S)-RvE3 in cytosol.", "equation": "18(S)-HEPE + O2 → 18(S)-RvE3", "inputs": [ { "node_id": "R-ALL-9020474", "reactome_id": "R-ALL-9020474", "label": "18(S)-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132083", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9022679", "reactome_id": "R-ALL-9022679", "label": "18(S)-RvE3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747565", "formula": "C20H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15 [cytosol]", "activity_id": "9022673", "ec_numbers": [ "1.13.11.33" ], "accessions": [ "P16050" ] } ], "citations": [ { "id": "PMID:22275352", "title": "Identification and structure determination of novel anti-inflammatory mediator resolvin E3, 17,18-dihydroxyeicosapentaenoic acid", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22275352/" }, { "id": "PMID:23293324", "title": "Stereochemical assignment and anti-inflammatory properties of the omega-3 lipid mediator resolvin E3", "journal": "J. Biochem.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23293324/" } ], "x": 5670, "y": 2211.1309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9023968", "label": "HPGD dimer oxidises 18(S)-RvE1 to 18-oxo-RvE1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9023968", "summary": "HPGD dimer oxidises 18(S)-RvE1 to 18-oxo-RvE1 is a Reactome release 97 curated reaction event. The source record connects 18(S)-RvE1, NAD+ to 18-oxo-RvE1, NADH in cytosol.", "equation": "18(S)-RvE1 + NAD+ → 18-oxo-RvE1 + NADH", "inputs": [ { "node_id": "R-ALL-9022660", "reactome_id": "R-ALL-9022660", "label": "18(S)-RvE1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:136057", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9023963", "reactome_id": "R-ALL-9023963", "label": "18-oxo-RvE1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:91001", "formula": "C20H27O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "15-hydroxyprostaglandin dehydrogenase (NAD+) activity of HPGD dimer [cytosol]", "activity_id": "2161623", "ec_numbers": [ "1.1.1.141" ], "accessions": [] } ], "citations": [ { "id": "PMID:16757471", "title": "Metabolic inactivation of resolvin E1 and stabilization of its anti-inflammatory actions", "journal": "J. Biol. Chem.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16757471/" }, { "id": "PMID:18292578", "title": "Resolvin E1 metabolome in local inactivation during inflammation-resolution", "journal": "J. Immunol.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18292578/" } ], "x": 5670, "y": 2127.7738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9023980", "label": "18(S)-RvE1, E2 and E3 translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9023980", "summary": "18(S)-RvE1, E2 and E3 translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 18(S)-RvE1,E2,E3 to 18(S)-RvE1,E2,E3 in cytosol · extracellular region.", "equation": "18(S)-RvE1,E2,E3 → 18(S)-RvE1,E2,E3", "inputs": [ { "node_id": "R-ALL-9023985", "reactome_id": "R-ALL-9023985", "label": "18(S)-RvE1,E2,E3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9029501", "reactome_id": "R-ALL-9029501", "label": "18(S)-RvE1,E2,E3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11034610", "title": "Novel functional sets of lipid-derived mediators with antiinflammatory actions generated from omega-3 fatty acids via cyclooxygenase 2-nonsteroidal antiinflammatory drugs and transcellular processing", "journal": "J. Exp. Med.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11034610/" } ], "x": 5948.666666666667, "y": 2350.059523809524, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9023983", "label": "18(R)-RvE1, E2 and E3 translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9023983", "summary": "18(R)-RvE1, E2 and E3 translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 18(R)-RvE1,E2,E3 to 18(R)-RvE1,E2,E3 in cytosol · extracellular region.", "equation": "18(R)-RvE1,E2,E3 → 18(R)-RvE1,E2,E3", "inputs": [ { "node_id": "R-ALL-9023979", "reactome_id": "R-ALL-9023979", "label": "18(R)-RvE1,E2,E3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9023981", "reactome_id": "R-ALL-9023981", "label": "18(R)-RvE1,E2,E3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11034610", "title": "Novel functional sets of lipid-derived mediators with antiinflammatory actions generated from omega-3 fatty acids via cyclooxygenase 2-nonsteroidal antiinflammatory drugs and transcellular processing", "journal": "J. Exp. Med.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11034610/" } ], "x": 5391.333333333333, "y": 2359.3214285714284, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9024586", "label": "AT-RvD1-6 translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024586", "summary": "AT-RvD1-6 translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects AT-RvD1-6 to AT-RvD1-6 in cytosol · extracellular region.", "equation": "AT-RvD1-6 → AT-RvD1-6", "inputs": [ { "node_id": "R-ALL-9024582", "reactome_id": "R-ALL-9024582", "label": "AT-RvD1-6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024575", "reactome_id": "R-ALL-9024575", "label": "AT-RvD1-6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11034610", "title": "Novel functional sets of lipid-derived mediators with antiinflammatory actions generated from omega-3 fatty acids via cyclooxygenase 2-nonsteroidal antiinflammatory drugs and transcellular processing", "journal": "J. Exp. Med.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11034610/" } ], "x": 5670, "y": 2359.3214285714284, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9024624", "label": "Hydroperoxy reductase reduces 4(S)-Hp-17(R)-HDHA to AT-RvD6", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024624", "summary": "Hydroperoxy reductase reduces 4(S)-Hp-17(R)-HDHA to AT-RvD6 is a Reactome release 97 curated reaction event. The source record connects 4(S)-Hp-17(R)-HDHA, reduced acceptor to AT-RvD6, oxidised acceptor in cytosol.", "equation": "4(S)-Hp-17(R)-HDHA + reduced acceptor → AT-RvD6 + oxidised acceptor", "inputs": [ { "node_id": "R-ALL-9024576", "reactome_id": "R-ALL-9024576", "label": "4(S)-Hp-17(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747573", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359016", "reactome_id": "R-ALL-5359016", "label": "reduced acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024633", "reactome_id": "R-ALL-9024633", "label": "AT-RvD6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747596", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9024637", "reactome_id": "R-ALL-9024637", "label": "oxidised acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of Hydroperoxy reductase [cytosol]", "activity_id": "9024806", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" } ], "x": 5670, "y": 2294.4880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024630", "label": "Hydroperoxy reductase reduces 7(S)-Hp-17(R)-HDHA to AT-RvD5", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024630", "summary": "Hydroperoxy reductase reduces 7(S)-Hp-17(R)-HDHA to AT-RvD5 is a Reactome release 97 curated reaction event. The source record connects 7(S)-Hp-17(R)-HDHA, reduced acceptor to AT-RvD5, oxidised acceptor in cytosol.", "equation": "7(S)-Hp-17(R)-HDHA + reduced acceptor → AT-RvD5 + oxidised acceptor", "inputs": [ { "node_id": "R-ALL-9024479", "reactome_id": "R-ALL-9024479", "label": "7(S)-Hp-17(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747574", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359016", "reactome_id": "R-ALL-5359016", "label": "reduced acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024639", "reactome_id": "R-ALL-9024639", "label": "AT-RvD5", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747578", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9024637", "reactome_id": "R-ALL-9024637", "label": "oxidised acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of Hydroperoxy reductase [cytosol]", "activity_id": "9024806", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" }, { "id": "PMID:28098462", "title": "Total Synthesis of Resolvin D5", "journal": "J. Org. Chem.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28098462/" } ], "x": 5948.666666666667, "y": 2294.4880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024766", "label": "HPGD dimer oxidises RvD1 to 17(S)-oxo-RvD1 and 8-oxo-17(S)-RvD1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:50129", "RHEA:50125" ], "source_url": "https://reactome.org/content/detail/R-HSA-9024766", "summary": "HPGD dimer oxidises RvD1 to 17(S)-oxo-RvD1 and 8-oxo-17(S)-RvD1 is a Reactome release 97 curated reaction event. The source record connects RvD1, NAD+ to 17(S)-oxo-RvD1, 8-oxo-17(S)-RvD1, NADH, H+ in cytosol and cross-references RHEA:50129, RHEA:50125.", "equation": "RvD1 + NAD+ → 17(S)-oxo-RvD1, 8-oxo-17(S)-RvD1 + NADH + H+", "inputs": [ { "node_id": "R-ALL-9024764", "reactome_id": "R-ALL-9024764", "label": "RvD1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:132079", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024799", "reactome_id": "R-ALL-9024799", "label": "17(S)-oxo-RvD1, 8-oxo-17(S)-RvD1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "15-hydroxyprostaglandin dehydrogenase (NAD+) activity of HPGD dimer [cytosol]", "activity_id": "2161623", "ec_numbers": [ "1.1.1.141" ], "accessions": [] } ], "citations": [ { "id": "PMID:17244615", "title": "Resolvin D1 and its aspirin-triggered 17R epimer. Stereochemical assignments, anti-inflammatory properties, and enzymatic inactivation", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17244615/" } ], "x": 5948.666666666667, "y": 2035.154761904762, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024785", "label": "RvD1-6 translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024785", "summary": "RvD1-6 translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects RvD1-6 to RvD1-6 in cytosol · extracellular region.", "equation": "RvD1-6 → RvD1-6", "inputs": [ { "node_id": "R-ALL-9024814", "reactome_id": "R-ALL-9024814", "label": "RvD1-6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024812", "reactome_id": "R-ALL-9024812", "label": "RvD1-6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11034610", "title": "Novel functional sets of lipid-derived mediators with antiinflammatory actions generated from omega-3 fatty acids via cyclooxygenase 2-nonsteroidal antiinflammatory drugs and transcellular processing", "journal": "J. Exp. Med.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11034610/" } ], "x": 5948.666666666667, "y": 2359.3214285714284, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9024872", "label": "ALOX15 oxidises 17(S)-Hp-DHA to PDX", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024872", "summary": "ALOX15 oxidises 17(S)-Hp-DHA to PDX is a Reactome release 97 curated reaction event. The source record connects 17(S)-Hp-DHA, O2 to PDX in cytosol.", "equation": "17(S)-Hp-DHA + O2 → PDX", "inputs": [ { "node_id": "R-ALL-9024012", "reactome_id": "R-ALL-9024012", "label": "17(S)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133795", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024883", "reactome_id": "R-ALL-9024883", "label": "PDX", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:138653", "formula": "C22H32O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15 [cytosol]", "activity_id": "9022673", "ec_numbers": [ "1.13.11.33" ], "accessions": [ "P16050" ] } ], "citations": [ { "id": "PMID:16424216", "title": "Anti-inflammatory actions of neuroprotectin D1/protectin D1 and its natural stereoisomers: assignments of dihydroxy-containing docosatrienes", "journal": "J. Immunol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16424216/" }, { "id": "PMID:12590139", "title": "Novel docosatrienes and 17S-resolvins generated from docosahexaenoic acid in murine brain, human blood, and glial cells. Autacoids in anti-inflammation", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12590139/" }, { "id": "PMID:19818771", "title": "Full characterization of PDX, a neuroprotectin/protectin D1 isomer, which inhibits blood platelet aggregation", "journal": "FEBS Lett.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19818771/" } ], "x": 5948.666666666667, "y": 2211.1309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024881", "label": "ALOX15 dehydrogenates 17(S)-Hp-DHA to 16S,17S-epoxy-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024881", "summary": "ALOX15 dehydrogenates 17(S)-Hp-DHA to 16S,17S-epoxy-DHA is a Reactome release 97 curated reaction event. The source record connects 17(S)-Hp-DHA, NADP+ to 16S,17S-epoxy-DHA, NADPH in cytosol.", "equation": "17(S)-Hp-DHA + NADP+ → 16S,17S-epoxy-DHA + NADPH", "inputs": [ { "node_id": "R-ALL-9024012", "reactome_id": "R-ALL-9024012", "label": "17(S)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133795", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024873", "reactome_id": "R-ALL-9024873", "label": "16S,17S-epoxy-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140313", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15 [cytosol]", "activity_id": "9022673", "ec_numbers": [ "1.13.11.33" ], "accessions": [ "P16050" ] } ], "citations": [ { "id": "PMID:26580578", "title": "Synthesis of the 16S,17S-Epoxyprotectin Intermediate in the Biosynthesis of Protectins by Human Macrophages", "journal": "J. Nat. Prod.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26580578/" }, { "id": "PMID:12590139", "title": "Novel docosatrienes and 17S-resolvins generated from docosahexaenoic acid in murine brain, human blood, and glial cells. Autacoids in anti-inflammation", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12590139/" }, { "id": "PMID:17055291", "title": "Resolvin D1, protectin D1, and related docosahexaenoic acid-derived products: Analysis via electrospray/low energy tandem mass spectrometry based on spectra and fragmentation mechanisms", "journal": "J. Am. Soc. Mass Spectrom.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17055291/" }, { "id": "PMID:26878209", "title": "The Protectin PCTR1 Is Produced by Human M2 Macrophages and Enhances Resolution of Infectious Inflammation", "journal": "Am. J. Pathol.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26878209/" }, { "id": "PMID:16424216", "title": "Anti-inflammatory actions of neuroprotectin D1/protectin D1 and its natural stereoisomers: assignments of dihydroxy-containing docosatrienes", "journal": "J. Immunol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16424216/" } ], "x": 5948.666666666667, "y": 2127.7738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024890", "label": "LTA4H:Zn2+ hydrolyses 16S,17S-epoxy-DHA to (N)PD1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024890", "summary": "LTA4H:Zn2+ hydrolyses 16S,17S-epoxy-DHA to (N)PD1 is a Reactome release 97 curated reaction event. The source record connects 16S,17S-epoxy-DHA, H2O to (N)PD1 in cytosol.", "equation": "16S,17S-epoxy-DHA + H2O → (N)PD1", "inputs": [ { "node_id": "R-ALL-9024873", "reactome_id": "R-ALL-9024873", "label": "16S,17S-epoxy-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140313", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024886", "reactome_id": "R-ALL-9024886", "label": "(N)PD1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195348", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of LTA4H:Zn2+ [cytosol]", "activity_id": "9022649", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:16424216", "title": "Anti-inflammatory actions of neuroprotectin D1/protectin D1 and its natural stereoisomers: assignments of dihydroxy-containing docosatrienes", "journal": "J. Immunol.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16424216/" }, { "id": "PMID:12590139", "title": "Novel docosatrienes and 17S-resolvins generated from docosahexaenoic acid in murine brain, human blood, and glial cells. Autacoids in anti-inflammation", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12590139/" } ], "x": 5391.333333333333, "y": 2220.3928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024973", "label": "Epoxide hydrolase hydrolyses 13(S),14(S)-epoxy-DHA to MaR1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024973", "summary": "Epoxide hydrolase hydrolyses 13(S),14(S)-epoxy-DHA to MaR1 is a Reactome release 97 curated reaction event. The source record connects 13(S),14(S)-epoxy-DHA, H2O to MaR1 in cytosol.", "equation": "13(S),14(S)-epoxy-DHA + H2O → MaR1", "inputs": [ { "node_id": "R-ALL-9025001", "reactome_id": "R-ALL-9025001", "label": "13(S),14(S)-epoxy-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:131958", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9025000", "reactome_id": "R-ALL-9025000", "label": "MaR1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:138249", "formula": "C22H32O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of Epoxide hydrolase [cytosol]", "activity_id": "9025026", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:23504711", "title": "The novel 13S,14S-epoxy-maresin is converted by human macrophages to maresin 1 (MaR1), inhibits leukotriene A4 hydrolase (LTA4H), and shifts macrophage phenotype", "journal": "FASEB J.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23504711/" }, { "id": "PMID:19103881", "title": "Maresins: novel macrophage mediators with potent antiinflammatory and proresolving actions", "journal": "J. Exp. Med.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19103881/" }, { "id": "PMID:25409514", "title": "The pro-resolving lipid mediator maresin 1 (MaR1) attenuates inflammatory signaling pathways in vascular smooth muscle and endothelial cells", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25409514/" }, { "id": "PMID:22253477", "title": "Macrophage proresolving mediator maresin 1 stimulates tissue regeneration and controls pain", "journal": "FASEB J.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22253477/" }, { "id": "PMID:25036362", "title": "Maresin biosynthesis and identification of maresin 2, a new anti-inflammatory and pro-resolving mediator from human macrophages", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25036362/" } ], "x": 5670, "y": 2220.3928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024983", "label": "ALOX12:Fe2+ dehydrogenates 14(S)-Hp-DHA to 13(S),14(S)-epoxy-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024983", "summary": "ALOX12:Fe2+ dehydrogenates 14(S)-Hp-DHA to 13(S),14(S)-epoxy-DHA is a Reactome release 97 curated reaction event. The source record connects 14(S)-Hp-DHA, NADP+ to 13(S),14(S)-epoxy-DHA, NADPH in cytosol.", "equation": "14(S)-Hp-DHA + NADP+ → 13(S),14(S)-epoxy-DHA + NADPH", "inputs": [ { "node_id": "R-ALL-9025012", "reactome_id": "R-ALL-9025012", "label": "14(S)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140279", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9025001", "reactome_id": "R-ALL-9025001", "label": "13(S),14(S)-epoxy-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:131958", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "arachidonate 12(S)-lipoxygenase activity of ALOX12:Fe2+ [cytosol]", "activity_id": "2161926", "ec_numbers": [ "1.13.11.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:23504711", "title": "The novel 13S,14S-epoxy-maresin is converted by human macrophages to maresin 1 (MaR1), inhibits leukotriene A4 hydrolase (LTA4H), and shifts macrophage phenotype", "journal": "FASEB J.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23504711/" }, { "id": "PMID:19103881", "title": "Maresins: novel macrophage mediators with potent antiinflammatory and proresolving actions", "journal": "J. Exp. Med.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19103881/" }, { "id": "PMID:25036362", "title": "Maresin biosynthesis and identification of maresin 2, a new anti-inflammatory and pro-resolving mediator from human macrophages", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25036362/" } ], "x": 5391.333333333333, "y": 2137.035714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024993", "label": "EPHX2 dimer hydrolyses 13(S),14(S)-epoxy-DHA to MaR2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:53537" ], "source_url": "https://reactome.org/content/detail/R-HSA-9024993", "summary": "EPHX2 dimer hydrolyses 13(S),14(S)-epoxy-DHA to MaR2 is a Reactome release 97 curated reaction event. The source record connects 13(S),14(S)-epoxy-DHA, H2O to MaR2 in cytosol and cross-references RHEA:53537.", "equation": "13(S),14(S)-epoxy-DHA + H2O → MaR2", "inputs": [ { "node_id": "R-ALL-9025001", "reactome_id": "R-ALL-9025001", "label": "13(S),14(S)-epoxy-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:131958", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9025011", "reactome_id": "R-ALL-9025011", "label": "MaR2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:137442", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of EPHX2 dimer [cytosol]", "activity_id": "2161846", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:25036362", "title": "Maresin biosynthesis and identification of maresin 2, a new anti-inflammatory and pro-resolving mediator from human macrophages", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25036362/" } ], "x": 5391.333333333333, "y": 2062.940476190476, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9024997", "label": "ALOX5 oxidises 14(S)-Hp-DHA to 7(S),14(S)-diHp-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9024997", "summary": "ALOX5 oxidises 14(S)-Hp-DHA to 7(S),14(S)-diHp-DHA is a Reactome release 97 curated reaction event. The source record connects 14(S)-Hp-DHA, O2 to 7(S),14(S)-diHp-DHA in cytosol.", "equation": "14(S)-Hp-DHA + O2 → 7(S),14(S)-diHp-DHA", "inputs": [ { "node_id": "R-ALL-9025012", "reactome_id": "R-ALL-9025012", "label": "14(S)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140279", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9024989", "reactome_id": "R-ALL-9024989", "label": "7(S),14(S)-diHp-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140196", "formula": "C22H32O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:19103881", "title": "Maresins: novel macrophage mediators with potent antiinflammatory and proresolving actions", "journal": "J. Exp. Med.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19103881/" } ], "x": 5948.666666666667, "y": 2220.3928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9025007", "label": "Hydroperoxy reductase reduces 7(S),14(S)-diHp-DHA to 7-epi-MaR1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9025007", "summary": "Hydroperoxy reductase reduces 7(S),14(S)-diHp-DHA to 7-epi-MaR1 is a Reactome release 97 curated reaction event. The source record connects 7(S),14(S)-diHp-DHA, reduced acceptor to 7-epi-MaR1, oxidised acceptor in cytosol.", "equation": "7(S),14(S)-diHp-DHA + reduced acceptor → 7-epi-MaR1 + oxidised acceptor", "inputs": [ { "node_id": "R-ALL-9024989", "reactome_id": "R-ALL-9024989", "label": "7(S),14(S)-diHp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140196", "formula": "C22H32O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359016", "reactome_id": "R-ALL-5359016", "label": "reduced acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9025005", "reactome_id": "R-ALL-9025005", "label": "7-epi-MaR1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140350", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9024637", "reactome_id": "R-ALL-9024637", "label": "oxidised acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of Hydroperoxy reductase [cytosol]", "activity_id": "9024806", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:19103881", "title": "Maresins: novel macrophage mediators with potent antiinflammatory and proresolving actions", "journal": "J. Exp. Med.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19103881/" }, { "id": "PMID:20708099", "title": "Specialized pro-resolving lipid mediators in the inflammatory response: An update", "journal": "Biochim. Biophys. Acta", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20708099/" } ], "x": 5391.333333333333, "y": 2303.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9025152", "label": "ALOX15 oxidises DPAn-6 to 17(S)-HDPAn-6 and 10(S),17(S)-diHDPAn-6", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9025152", "summary": "ALOX15 oxidises DPAn-6 to 17(S)-HDPAn-6 and 10(S),17(S)-diHDPAn-6 is a Reactome release 97 curated reaction event. The source record connects O2, DPAn-6 to 17(S)-HDPAn-6, 10(S),17(S)-diHDPAn-6 in cytosol.", "equation": "O2 + DPAn-6 → 17(S)-HDPAn-6, 10(S),17(S)-diHDPAn-6", "inputs": [ { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9025155", "reactome_id": "R-ALL-9025155", "label": "DPAn-6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77226", "formula": "C22H33O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9025151", "reactome_id": "R-ALL-9025151", "label": "17(S)-HDPAn-6, 10(S),17(S)-diHDPAn-6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15 [cytosol]", "activity_id": "9022673", "ec_numbers": [ "1.13.11.33" ], "accessions": [ "P16050" ] } ], "citations": [ { "id": "PMID:19324874", "title": "Biogenic synthesis, purification, and chemical characterization of anti-inflammatory resolvins derived from docosapentaenoic acid (DPAn-6)", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19324874/" }, { "id": "PMID:19679107", "title": "Metabolism and biological production of resolvins derived from docosapentaenoic acid (DPAn-6)", "journal": "Biochem. Pharmacol.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19679107/" }, { "id": "PMID:20364438", "title": "Docosahexaenoic acid (DHA) and docosapentaenoic acid (DPAn-6) algal oils reduce inflammatory mediators in human peripheral mononuclear cells in vitro and paw edema in vivo", "journal": "Lipids", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20364438/" }, { "id": "PMID:23471029", "title": "Controlled formation of mono- and dihydroxy-resolvins from EPA and DHA using soybean 15-lipoxygenase", "journal": "J. Lipid Res.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23471029/" }, { "id": "PMID:24571431", "title": "Total synthesis of neuroprotectin D1 analogues derived from omega-6 docosapentaenoic acid (DPA) and adrenic acid (AdA) from a common pivotal, late-stage intermediate", "journal": "J. Org. Chem.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24571431/" } ], "x": 5670, "y": 2303.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9025957", "label": "ALOX12:Fe2+ oxidises DPAn-6 to 14(S)-HDPAn-6", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9025957", "summary": "ALOX12:Fe2+ oxidises DPAn-6 to 14(S)-HDPAn-6 is a Reactome release 97 curated reaction event. The source record connects O2, DPAn-6 to 14(S)-HDPAn-6 in cytosol.", "equation": "O2 + DPAn-6 → 14(S)-HDPAn-6", "inputs": [ { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9025155", "reactome_id": "R-ALL-9025155", "label": "DPAn-6", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77226", "formula": "C22H33O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9025960", "reactome_id": "R-ALL-9025960", "label": "14(S)-HDPAn-6", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140251", "formula": "C22H33O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 12(S)-lipoxygenase activity of ALOX12:Fe2+ [cytosol]", "activity_id": "2161926", "ec_numbers": [ "1.13.11.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:19324874", "title": "Biogenic synthesis, purification, and chemical characterization of anti-inflammatory resolvins derived from docosapentaenoic acid (DPAn-6)", "journal": "J. Biol. Chem.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19324874/" }, { "id": "PMID:19679107", "title": "Metabolism and biological production of resolvins derived from docosapentaenoic acid (DPAn-6)", "journal": "Biochem. Pharmacol.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19679107/" }, { "id": "PMID:23471029", "title": "Controlled formation of mono- and dihydroxy-resolvins from EPA and DHA using soybean 15-lipoxygenase", "journal": "J. Lipid Res.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23471029/" } ], "x": 5391.333333333333, "y": 2229.654761904762, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9025995", "label": "ALOX5 dehydrogenates 7,17-diHp-DPAn-3 to 7,8-epoxy,17-HDPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9025995", "summary": "ALOX5 dehydrogenates 7,17-diHp-DPAn-3 to 7,8-epoxy,17-HDPAn-3 is a Reactome release 97 curated reaction event. The source record connects 7,17-diHp-DPAn-3, NADP+ to 7,8-epoxy,17-HDPAn-3, NADPH in cytosol.", "equation": "7,17-diHp-DPAn-3 + NADP+ → 7,8-epoxy,17-HDPAn-3 + NADPH", "inputs": [ { "node_id": "R-ALL-9026034", "reactome_id": "R-ALL-9026034", "label": "7,17-diHp-DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140381", "formula": "C22H33O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026050", "reactome_id": "R-ALL-9026050", "label": "7,8-epoxy,17-HDPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140293", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" } ], "x": 5670, "y": 2137.035714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9025996", "label": "ALOX5 oxidises 17(S)-Hp-DPAn-3 to 7,17-diHp-DPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9025996", "summary": "ALOX5 oxidises 17(S)-Hp-DPAn-3 to 7,17-diHp-DPAn-3 is a Reactome release 97 curated reaction event. The source record connects 17(S)-Hp-DPAn-3, O2 to 7,17-diHp-DPAn-3 in cytosol.", "equation": "17(S)-Hp-DPAn-3 + O2 → 7,17-diHp-DPAn-3", "inputs": [ { "node_id": "R-ALL-9026047", "reactome_id": "R-ALL-9026047", "label": "17(S)-Hp-DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140291", "formula": "C22H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026034", "reactome_id": "R-ALL-9026034", "label": "7,17-diHp-DPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140381", "formula": "C22H33O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" } ], "x": 5670, "y": 2229.654761904762, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9025998", "label": "Epoxide hydrolase hydrolyses 13,14-epoxy-DPAn-3 to MaR1n-3 DPA or MaR2n-3 DPA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9025998", "summary": "Epoxide hydrolase hydrolyses 13,14-epoxy-DPAn-3 to MaR1n-3 DPA or MaR2n-3 DPA is a Reactome release 97 curated reaction event. The source record connects 13,14(S)-epoxy-DPAn-3, H2O to MaR1n-3 DPA, MaR2n-3 DPA in cytosol.", "equation": "13,14(S)-epoxy-DPAn-3 + H2O → MaR1n-3 DPA, MaR2n-3 DPA", "inputs": [ { "node_id": "R-ALL-9026307", "reactome_id": "R-ALL-9026307", "label": "13,14(S)-epoxy-DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140235", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026321", "reactome_id": "R-ALL-9026321", "label": "MaR1n-3 DPA, MaR2n-3 DPA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of Epoxide hydrolase [cytosol]", "activity_id": "9025026", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" }, { "id": "PMID:25225129", "title": "Total synthesis of the anti-inflammatory and pro-resolving lipid mediator MaR1n-3 DPA utilizing an sp(3) -sp(3) Negishi cross-coupling reaction", "journal": "Chemistry", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25225129/" } ], "x": 5948.666666666667, "y": 2303.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9025999", "label": "ALOX5 dehydrogenates 17(S)-Hp-DPAn-3 to 16(S),17(S)-epoxy-DPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9025999", "summary": "ALOX5 dehydrogenates 17(S)-Hp-DPAn-3 to 16(S),17(S)-epoxy-DPAn-3 is a Reactome release 97 curated reaction event. The source record connects 17(S)-Hp-DPAn-3, NADP+ to 16(S),17(S)-epoxy-DPAn-3, NADPH in cytosol.", "equation": "17(S)-Hp-DPAn-3 + NADP+ → 16(S),17(S)-epoxy-DPAn-3 + NADPH", "inputs": [ { "node_id": "R-ALL-9026047", "reactome_id": "R-ALL-9026047", "label": "17(S)-Hp-DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140291", "formula": "C22H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026046", "reactome_id": "R-ALL-9026046", "label": "16(S),17(S)-epoxy-DPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140290", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:28980694", "title": "Stereocontrolled synthesis and investigation of the biosynthetic transformations of 16(S),17(S)-epoxy-PDn-3 DPA", "journal": "Org. 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The source record connects 16(S),17(S)-epoxy-DPAn-3, H2O to PD1n-3DPA,PD2n-3DPA in cytosol.", "equation": "16(S),17(S)-epoxy-DPAn-3 + H2O → PD1n-3DPA,PD2n-3DPA", "inputs": [ { "node_id": "R-ALL-9026046", "reactome_id": "R-ALL-9026046", "label": "16(S),17(S)-epoxy-DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140290", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026023", "reactome_id": "R-ALL-9026023", "label": "PD1n-3DPA,PD2n-3DPA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of Epoxide hydrolase [cytosol]", "activity_id": "9025026", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:28980694", "title": "Stereocontrolled synthesis and investigation of the biosynthetic transformations of 16(S),17(S)-epoxy-PDn-3 DPA", "journal": "Org. 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Prod.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24576195/" } ], "x": 5391.333333333333, "y": 2313.0119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026001", "label": "Hydroperoxy reductase reduces 7,17-diHp-DPAn-3 to RvD5n-3DPA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026001", "summary": "Hydroperoxy reductase reduces 7,17-diHp-DPAn-3 to RvD5n-3DPA is a Reactome release 97 curated reaction event. The source record connects 7,17-diHp-DPAn-3, reduced acceptor to RvD5n-3DPA, oxidised acceptor in cytosol.", "equation": "7,17-diHp-DPAn-3 + reduced acceptor → RvD5n-3DPA + oxidised acceptor", "inputs": [ { "node_id": "R-ALL-9026034", "reactome_id": "R-ALL-9026034", "label": "7,17-diHp-DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140381", "formula": "C22H33O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359016", "reactome_id": "R-ALL-5359016", "label": "reduced acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026036", "reactome_id": "R-ALL-9026036", "label": "RvD5n-3DPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140419", "formula": "C22H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9024637", "reactome_id": "R-ALL-9024637", "label": "oxidised acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of Hydroperoxy reductase [cytosol]", "activity_id": "9024806", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" } ], "x": 5670, "y": 2313.0119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026003", "label": "ALOX15 oxidises DPAn-3 to 17(S)-Hp-DPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026003", "summary": "ALOX15 oxidises DPAn-3 to 17(S)-Hp-DPAn-3 is a Reactome release 97 curated reaction event. The source record connects DPAn-3, O2 to 17(S)-Hp-DPAn-3 in cytosol.", "equation": "DPAn-3 + O2 → 17(S)-Hp-DPAn-3", "inputs": [ { "node_id": "R-ALL-9608444", "reactome_id": "R-ALL-9608444", "label": "DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77224", "formula": "C22H33O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026047", "reactome_id": "R-ALL-9026047", "label": "17(S)-Hp-DPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140291", "formula": "C22H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 15-lipoxygenase activity of ALOX15 [cytosol]", "activity_id": "9022673", "ec_numbers": [ "1.13.11.33" ], "accessions": [ "P16050" ] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" }, { "id": "PMID:28980694", "title": "Stereocontrolled synthesis and investigation of the biosynthetic transformations of 16(S),17(S)-epoxy-PDn-3 DPA", "journal": "Org. Biomol. Chem.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28980694/" }, { "id": "PMID:24576195", "title": "Total synthesis of the lipid mediator PD1n-3 DPA: configurational assignments and anti-inflammatory and pro-resolving actions", "journal": "J. Nat. Prod.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24576195/" } ], "x": 5948.666666666667, "y": 2229.654761904762, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026005", "label": "ALOX5 oxidises 14(S)-Hp-DPAn-3 to MaR3n-3 DPA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026005", "summary": "ALOX5 oxidises 14(S)-Hp-DPAn-3 to MaR3n-3 DPA is a Reactome release 97 curated reaction event. The source record connects 14(S)-Hp-DPAn-3, O2 to MaR3n-3 DPA in cytosol.", "equation": "14(S)-Hp-DPAn-3 + O2 → MaR3n-3 DPA", "inputs": [ { "node_id": "R-ALL-9026293", "reactome_id": "R-ALL-9026293", "label": "14(S)-Hp-DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140289", "formula": "C22H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026311", "reactome_id": "R-ALL-9026311", "label": "MaR3n-3 DPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140393", "formula": "C22H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" }, { "id": "PMID:28408222", "title": "Recent advances in the chemistry and biology of anti-inflammatory and specialized pro-resolving mediators biosynthesized from n-3 docosapentaenoic acid", "journal": "Bioorg. Med. Chem. Lett.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28408222/" } ], "x": 5391.333333333333, "y": 2238.9166666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026006", "label": "ALOX12:Fe2+ oxidises DPAn-3 to 14(S)-Hp-DPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026006", "summary": "ALOX12:Fe2+ oxidises DPAn-3 to 14(S)-Hp-DPAn-3 is a Reactome release 97 curated reaction event. The source record connects DPAn-3, O2 to 14(S)-Hp-DPAn-3 in cytosol.", "equation": "DPAn-3 + O2 → 14(S)-Hp-DPAn-3", "inputs": [ { "node_id": "R-ALL-9608444", "reactome_id": "R-ALL-9608444", "label": "DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77224", "formula": "C22H33O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026293", "reactome_id": "R-ALL-9026293", "label": "14(S)-Hp-DPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140289", "formula": "C22H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "arachidonate 12(S)-lipoxygenase activity of ALOX12:Fe2+ [cytosol]", "activity_id": "2161926", "ec_numbers": [ "1.13.11.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" } ], "x": 5670, "y": 2238.9166666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026007", "label": "ALOX12:Fe2+ dehydrogenates 14(S)-Hp-DPAn-3 to 13,14-epoxy-DPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026007", "summary": "ALOX12:Fe2+ dehydrogenates 14(S)-Hp-DPAn-3 to 13,14-epoxy-DPAn-3 is a Reactome release 97 curated reaction event. The source record connects 14(S)-Hp-DHA, NADP+ to 13,14(S)-epoxy-DPAn-3, NADPH in cytosol.", "equation": "14(S)-Hp-DHA + NADP+ → 13,14(S)-epoxy-DPAn-3 + NADPH", "inputs": [ { "node_id": "R-ALL-9025012", "reactome_id": "R-ALL-9025012", "label": "14(S)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140279", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026307", "reactome_id": "R-ALL-9026307", "label": "13,14(S)-epoxy-DPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140235", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "arachidonate 12(S)-lipoxygenase activity of ALOX12:Fe2+ [cytosol]", "activity_id": "2161926", "ec_numbers": [ "1.13.11.31" ], "accessions": [] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" } ], "x": 5391.333333333333, "y": 2146.2976190476193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026008", "label": "Epoxide hydrolase hydrolyses 7,8-epoxy-HDPAn-3 to RvD1n-3DPA or RvD2n-3DPA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026008", "summary": "Epoxide hydrolase hydrolyses 7,8-epoxy-HDPAn-3 to RvD1n-3DPA or RvD2n-3DPA is a Reactome release 97 curated reaction event. The source record connects 7,8-epoxy,17-HDPAn-3, H2O to RvD1n-3DPA, RvD2n-3DPA in cytosol.", "equation": "7,8-epoxy,17-HDPAn-3 + H2O → RvD1n-3DPA, RvD2n-3DPA", "inputs": [ { "node_id": "R-ALL-9026050", "reactome_id": "R-ALL-9026050", "label": "7,8-epoxy,17-HDPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140293", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026291", "reactome_id": "R-ALL-9026291", "label": "RvD1n-3DPA, RvD2n-3DPA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of Epoxide hydrolase [cytosol]", "activity_id": "9025026", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" } ], "x": 5948.666666666667, "y": 2313.0119047619046, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026405", "label": "ALOX5 oxidises 13(R)-HDPAn-3 to RvT1-4", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026405", "summary": "ALOX5 oxidises 13(R)-HDPAn-3 to RvT1-4 is a Reactome release 97 curated reaction event. The source record connects 13(R)-HDPAn-3, O2 to RvT1-4 in cytosol.", "equation": "13(R)-HDPAn-3 + O2 → RvT1-4", "inputs": [ { "node_id": "R-ALL-9026400", "reactome_id": "R-ALL-9026400", "label": "13(R)-HDPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90824", "formula": "C22H33O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026394", "reactome_id": "R-ALL-9026394", "label": "RvT1-4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:26236990", "title": "Elucidation of novel 13-series resolvins that increase with atorvastatin and clear infections", "journal": "Nat. Med.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26236990/" }, { "id": "PMID:27704804", "title": "Synthesis of 13(R)-Hydroxy-7Z,10Z,13R,14E,16Z,19Z Docosapentaenoic Acid (13R-HDPA) and Its Biosynthetic Conversion to the 13-Series Resolvins", "journal": "J. Nat. Prod.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27704804/" } ], "x": 5391.333333333333, "y": 2322.2738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026408", "label": "PTGS2 dimer oxidises DPAn-3 to 13(R)-HDPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:48853" ], "source_url": "https://reactome.org/content/detail/R-HSA-9026408", "summary": "PTGS2 dimer oxidises DPAn-3 to 13(R)-HDPAn-3 is a Reactome release 97 curated reaction event. The source record connects DPAn-3, O2, hydrogen donor to 13(R)-HDPAn-3, hydrogen acceptor, H2O in cytosol · endoplasmic reticulum membrane and cross-references RHEA:48853.", "equation": "DPAn-3 + O2 + hydrogen donor → 13(R)-HDPAn-3 + hydrogen acceptor + H2O", "inputs": [ { "node_id": "R-ALL-9608444", "reactome_id": "R-ALL-9608444", "label": "DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77224", "formula": "C22H33O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1131953", "reactome_id": "R-ALL-1131953", "label": "hydrogen donor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17499", "formula": "H2A", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026407", "reactome_id": "R-ALL-9026407", "label": "13(R)-HDPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90824", "formula": "C22H33O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1130886", "reactome_id": "R-ALL-1130886", "label": "hydrogen acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:13193", "formula": "A", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen of PTGS2 dimer [endoplasmic reticulum membrane]", "activity_id": "9026406", "ec_numbers": [ "1.13.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:26236990", "title": "Elucidation of novel 13-series resolvins that increase with atorvastatin and clear infections", "journal": "Nat. Med.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26236990/" }, { "id": "PMID:27704804", "title": "Synthesis of 13(R)-Hydroxy-7Z,10Z,13R,14E,16Z,19Z Docosapentaenoic Acid (13R-HDPA) and Its Biosynthetic Conversion to the 13-Series Resolvins", "journal": "J. Nat. Prod.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27704804/" } ], "x": 5391.333333333333, "y": 2016.6309523809523, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026411", "label": "13(R)-HDPAn-3 translocates from endothelial cell to neutrophil", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026411", "summary": "13(R)-HDPAn-3 translocates from endothelial cell to neutrophil is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 13(R)-HDPAn-3 to 13(R)-HDPAn-3 in cytosol.", "equation": "13(R)-HDPAn-3 → 13(R)-HDPAn-3", "inputs": [ { "node_id": "R-ALL-9026407", "reactome_id": "R-ALL-9026407", "label": "13(R)-HDPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90824", "formula": "C22H33O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026400", "reactome_id": "R-ALL-9026400", "label": "13(R)-HDPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90824", "formula": "C22H33O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:26236990", "title": "Elucidation of novel 13-series resolvins that increase with atorvastatin and clear infections", "journal": "Nat. Med.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26236990/" }, { "id": "PMID:27704804", "title": "Synthesis of 13(R)-Hydroxy-7Z,10Z,13R,14E,16Z,19Z Docosapentaenoic Acid (13R-HDPA) and Its Biosynthetic Conversion to the 13-Series Resolvins", "journal": "J. Nat. Prod.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27704804/" } ], "x": 5948.666666666667, "y": 2340.7976190476193, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9026544", "label": "Non-enzymatic hydrolysis hydrolyses 13(S),14(S)-epoxy-DHA to 7-epi-MaR1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026544", "summary": "Non-enzymatic hydrolysis hydrolyses 13(S),14(S)-epoxy-DHA to 7-epi-MaR1 is a Reactome release 97 curated reaction event. The source record connects 13(S),14(S)-epoxy-DHA, H2O to 7-epi-MaR1 in cytosol.", "equation": "13(S),14(S)-epoxy-DHA + H2O → 7-epi-MaR1", "inputs": [ { "node_id": "R-ALL-9025001", "reactome_id": "R-ALL-9025001", "label": "13(S),14(S)-epoxy-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:131958", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9025005", "reactome_id": "R-ALL-9025005", "label": "7-epi-MaR1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140350", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19103881", "title": "Maresins: novel macrophage mediators with potent antiinflammatory and proresolving actions", "journal": "J. Exp. Med.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19103881/" } ], "x": 5948.666666666667, "y": 2322.2738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026757", "label": "GGT hydrolyses MCTR1 to MCTR2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:53513" ], "source_url": "https://reactome.org/content/detail/R-HSA-9026757", "summary": "GGT hydrolyses MCTR1 to MCTR2 is a Reactome release 97 curated reaction event. The source record connects MCTR1, H2O to MCTR2, L-Glu in cytosol and cross-references RHEA:53513.", "equation": "MCTR1 + H2O → MCTR2 + L-Glu", "inputs": [ { "node_id": "R-ALL-9026761", "reactome_id": "R-ALL-9026761", "label": "MCTR1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:137407", "formula": "C32H45N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026760", "reactome_id": "R-ALL-9026760", "label": "MCTR2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:137408", "formula": "C27H39N2O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione gamma-glutamate hydrolase activity of GGT [cytosol]", "activity_id": "9026767", "ec_numbers": [ "3.4.19.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:25324525", "title": "Identification of 14-series sulfido-conjugated mediators that promote resolution of infection and organ protection", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25324525/" }, { "id": "PMID:27791009", "title": "Maresin conjugates in tissue regeneration biosynthesis enzymes in human macrophages", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27791009/" }, { "id": "PMID:26881986", "title": "Identification and Actions of a Novel Third Maresin Conjugate in Tissue Regeneration: MCTR3", "journal": "PLoS ONE", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26881986/" } ], "x": 5391.333333333333, "y": 2044.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026771", "label": "DPEP hydrolyses MCTR2 to MCTR3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:53541" ], "source_url": "https://reactome.org/content/detail/R-HSA-9026771", "summary": "DPEP hydrolyses MCTR2 to MCTR3 is a Reactome release 97 curated reaction event. The source record connects MCTR2, H2O to MCTR3, Gly in cytosol and cross-references RHEA:53541.", "equation": "MCTR2 + H2O → MCTR3 + Gly", "inputs": [ { "node_id": "R-ALL-9026760", "reactome_id": "R-ALL-9026760", "label": "MCTR2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:137408", "formula": "C27H39N2O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026776", "reactome_id": "R-ALL-9026776", "label": "MCTR3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:137410", "formula": "C25H36NO5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dipeptidase activity of DPEP [cytosol]", "activity_id": "9026769", "ec_numbers": [ "3.4.13.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:25324525", "title": "Identification of 14-series sulfido-conjugated mediators that promote resolution of infection and organ protection", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25324525/" }, { "id": "PMID:27791009", "title": "Maresin conjugates in tissue regeneration biosynthesis enzymes in human macrophages", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27791009/" }, { "id": "PMID:26881986", "title": "Identification and Actions of a Novel Third Maresin Conjugate in Tissue Regeneration: MCTR3", "journal": "PLoS ONE", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26881986/" } ], "x": 5670, "y": 2044.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026777", "label": "GSTM4 dimer transfers GSH to 13(S),14(S)-epoxy-DHA to form MCTR1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:53509" ], "source_url": "https://reactome.org/content/detail/R-HSA-9026777", "summary": "GSTM4 dimer transfers GSH to 13(S),14(S)-epoxy-DHA to form MCTR1 is a Reactome release 97 curated reaction event. The source record connects 13(S),14(S)-epoxy-DHA, GSH to MCTR1 in cytosol and cross-references RHEA:53509.", "equation": "13(S),14(S)-epoxy-DHA + GSH → MCTR1", "inputs": [ { "node_id": "R-ALL-9025001", "reactome_id": "R-ALL-9025001", "label": "13(S),14(S)-epoxy-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:131958", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026761", "reactome_id": "R-ALL-9026761", "label": "MCTR1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:137407", "formula": "C32H45N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione transferase activity of GSTM4 dimer [cytosol]", "activity_id": "9026765", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:27791009", "title": "Maresin conjugates in tissue regeneration biosynthesis enzymes in human macrophages", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27791009/" }, { "id": "PMID:25324525", "title": "Identification of 14-series sulfido-conjugated mediators that promote resolution of infection and organ protection", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25324525/" }, { "id": "PMID:26881986", "title": "Identification and Actions of a Novel Third Maresin Conjugate in Tissue Regeneration: MCTR3", "journal": "PLoS ONE", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26881986/" } ], "x": 5670, "y": 2062.940476190476, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026780", "label": "LTC4S trimer transfers GSH to 13(S),14(S)-epoxy-DHA to form MCTR1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:53509" ], "source_url": "https://reactome.org/content/detail/R-HSA-9026780", "summary": "LTC4S trimer transfers GSH to 13(S),14(S)-epoxy-DHA to form MCTR1 is a Reactome release 97 curated reaction event. The source record connects 13(S),14(S)-epoxy-DHA, GSH to MCTR1 in cytosol · endoplasmic reticulum membrane and cross-references RHEA:53509.", "equation": "13(S),14(S)-epoxy-DHA + GSH → MCTR1", "inputs": [ { "node_id": "R-ALL-9025001", "reactome_id": "R-ALL-9025001", "label": "13(S),14(S)-epoxy-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:131958", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026761", "reactome_id": "R-ALL-9026761", "label": "MCTR1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:137407", "formula": "C32H45N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione transferase activity of LTC4S trimer [endoplasmic reticulum membrane]", "activity_id": "9026768", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:27791009", "title": "Maresin conjugates in tissue regeneration biosynthesis enzymes in human macrophages", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27791009/" }, { "id": "PMID:25324525", "title": "Identification of 14-series sulfido-conjugated mediators that promote resolution of infection and organ protection", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25324525/" }, { "id": "PMID:26881986", "title": "Identification and Actions of a Novel Third Maresin Conjugate in Tissue Regeneration: MCTR3", "journal": "PLoS ONE", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26881986/" } ], "x": 5948.666666666667, "y": 2062.940476190476, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026901", "label": "GGT transfers GSH to 16S,17S-epoxy-DHA to form PCTR1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026901", "summary": "GGT transfers GSH to 16S,17S-epoxy-DHA to form PCTR1 is a Reactome release 97 curated reaction event. The source record connects 16S,17S-epoxy-DHA, GSH to PCTR1 in cytosol.", "equation": "16S,17S-epoxy-DHA + GSH → PCTR1", "inputs": [ { "node_id": "R-ALL-9024873", "reactome_id": "R-ALL-9024873", "label": "16S,17S-epoxy-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140313", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026903", "reactome_id": "R-ALL-9026903", "label": "PCTR1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140394", "formula": "C32H45N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione transferase activity of GGT [cytosol]", "activity_id": "9026925", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:26878209", "title": "The Protectin PCTR1 Is Produced by Human M2 Macrophages and Enhances Resolution of Infectious Inflammation", "journal": "Am. J. Pathol.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26878209/" }, { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" } ], "x": 5948.666666666667, "y": 2238.9166666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026907", "label": "GGT hydrolyses PCTR2 to PCTR3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026907", "summary": "GGT hydrolyses PCTR2 to PCTR3 is a Reactome release 97 curated reaction event. The source record connects PCTR2, H2O to PCTR3, Gly in cytosol.", "equation": "PCTR2 + H2O → PCTR3 + Gly", "inputs": [ { "node_id": "R-ALL-9026921", "reactome_id": "R-ALL-9026921", "label": "PCTR2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140397", "formula": "C27H39N2O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026906", "reactome_id": "R-ALL-9026906", "label": "PCTR3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140401", "formula": "C25H36NO5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione gamma-glutamate hydrolase activity of GGT [cytosol]", "activity_id": "9026767", "ec_numbers": [ "3.4.19.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" } ], "x": 5670, "y": 2146.2976190476193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026911", "label": "LTC4S trimer transfers GSH to 7S(8)-epoxy-17(S)-HDHA to form RCTR1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026911", "summary": "LTC4S trimer transfers GSH to 7S(8)-epoxy-17(S)-HDHA to form RCTR1 is a Reactome release 97 curated reaction event. The source record connects 7S(8)-epoxy-17(S)-HDHA, GSH to RCTR1 in cytosol.", "equation": "7S(8)-epoxy-17(S)-HDHA + GSH → RCTR1", "inputs": [ { "node_id": "R-ALL-9024768", "reactome_id": "R-ALL-9024768", "label": "7S(8)-epoxy-17(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747581", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29450", "reactome_id": "R-ALL-29450", "label": "GSH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57925", "formula": "C10H16N3O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026915", "reactome_id": "R-ALL-9026915", "label": "RCTR1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140409", "formula": "C32H45N3O10S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione transferase activity of LTC4S trimer [endoplasmic reticulum membrane]", "activity_id": "9026768", "ec_numbers": [ "2.5.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" } ], "x": 5391.333333333333, "y": 2248.1785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026912", "label": "GGT hydrolyses PCTR1 to PCTR2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026912", "summary": "GGT hydrolyses PCTR1 to PCTR2 is a Reactome release 97 curated reaction event. The source record connects PCTR1, H2O to PCTR2, L-Glu in cytosol.", "equation": "PCTR1 + H2O → PCTR2 + L-Glu", "inputs": [ { "node_id": "R-ALL-9026903", "reactome_id": "R-ALL-9026903", "label": "PCTR1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140394", "formula": "C32H45N3O9S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026921", "reactome_id": "R-ALL-9026921", "label": "PCTR2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140397", "formula": "C27H39N2O6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione gamma-glutamate hydrolase activity of GGT [cytosol]", "activity_id": "9026767", "ec_numbers": [ "3.4.19.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" } ], "x": 5948.666666666667, "y": 2146.2976190476193, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026916", "label": "GGT hydrolyses RCTR2 to RCTR3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026916", "summary": "GGT hydrolyses RCTR2 to RCTR3 is a Reactome release 97 curated reaction event. The source record connects RCTR2, H2O to RCTR3, Gly in cytosol.", "equation": "RCTR2 + H2O → RCTR3 + Gly", "inputs": [ { "node_id": "R-ALL-9026922", "reactome_id": "R-ALL-9026922", "label": "RCTR2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140412", "formula": "C27H39N2O7S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026914", "reactome_id": "R-ALL-9026914", "label": "RCTR3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140413", "formula": "C25H36NO6S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione gamma-glutamate hydrolase activity of GGT [cytosol]", "activity_id": "9026767", "ec_numbers": [ "3.4.19.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" } ], "x": 5391.333333333333, "y": 2155.559523809524, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026917", "label": "Lipoxygenase dehydrogenates 7(S),17(S)-diHp-DHA to 7S(8)-epoxy-17(S)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026917", "summary": "Lipoxygenase dehydrogenates 7(S),17(S)-diHp-DHA to 7S(8)-epoxy-17(S)-HDHA is a Reactome release 97 curated reaction event. The source record connects 7(S),17(S)-diHp-DHA, NADP+ to 7S(8)-epoxy-17(S)-HDHA, NADPH in cytosol.", "equation": "7(S),17(S)-diHp-DHA + NADP+ → 7S(8)-epoxy-17(S)-HDHA + NADPH", "inputs": [ { "node_id": "R-ALL-9026919", "reactome_id": "R-ALL-9026919", "label": "7(S),17(S)-diHp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140349", "formula": "C22H31O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9024768", "reactome_id": "R-ALL-9024768", "label": "7S(8)-epoxy-17(S)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:747581", "formula": "C22H29O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity of Lipoxygenase [cytosol]", "activity_id": "9026924", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" } ], "x": 5670, "y": 2155.559523809524, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026918", "label": "Lipoxygenase oxidises 17(S)-Hp-DHA to 7(S),17(S)-diHp-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026918", "summary": "Lipoxygenase oxidises 17(S)-Hp-DHA to 7(S),17(S)-diHp-DHA is a Reactome release 97 curated reaction event. The source record connects 17(S)-Hp-DHA, O2 to 7(S),17(S)-diHp-DHA in cytosol.", "equation": "17(S)-Hp-DHA + O2 → 7(S),17(S)-diHp-DHA", "inputs": [ { "node_id": "R-ALL-9024012", "reactome_id": "R-ALL-9024012", "label": "17(S)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133795", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9026919", "reactome_id": "R-ALL-9026919", "label": "7(S),17(S)-diHp-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140349", "formula": "C22H31O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen of Lipoxygenase [cytosol]", "activity_id": "9026905", "ec_numbers": [ "1.13.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" } ], "x": 5670, "y": 2248.1785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9026927", "label": "GGT hydrolyses RCTR1 to RCTR2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9026927", "summary": "GGT hydrolyses RCTR1 to RCTR2 is a Reactome release 97 curated reaction event. The source record connects RCTR1, H2O to RCTR2, L-Glu in cytosol.", "equation": "RCTR1 + H2O → RCTR2 + L-Glu", "inputs": [ { "node_id": "R-ALL-9026915", "reactome_id": "R-ALL-9026915", "label": "RCTR1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140409", "formula": "C32H45N3O10S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9026922", "reactome_id": "R-ALL-9026922", "label": "RCTR2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140412", "formula": "C27H39N2O7S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glutathione gamma-glutamate hydrolase activity of GGT [cytosol]", "activity_id": "9026767", "ec_numbers": [ "3.4.19.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" } ], "x": 5948.666666666667, "y": 2155.559523809524, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027033", "label": "Hydroperoxy reducatase reduces 14(S)-Hp-DHA to 14(S)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027033", "summary": "Hydroperoxy reducatase reduces 14(S)-Hp-DHA to 14(S)-HDHA is a Reactome release 97 curated reaction event. The source record connects 14(S)-Hp-DHA, reduced acceptor to 14(S)-HDHA, oxidised acceptor in cytosol.", "equation": "14(S)-Hp-DHA + reduced acceptor → 14(S)-HDHA + oxidised acceptor", "inputs": [ { "node_id": "R-ALL-9025012", "reactome_id": "R-ALL-9025012", "label": "14(S)-Hp-DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140279", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5359016", "reactome_id": "R-ALL-5359016", "label": "reduced acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9027029", "reactome_id": "R-ALL-9027029", "label": "14(S)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:136526", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9024637", "reactome_id": "R-ALL-9024637", "label": "oxidised acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of Hydroperoxy reductase [cytosol]", "activity_id": "9024806", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:25200603", "title": "Maresin-like lipid mediators are produced by leukocytes and platelets and rescue reparative function of diabetes-impaired macrophages", "journal": "Chem. Biol.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25200603/" } ], "x": 5670, "y": 2322.2738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027042", "label": "CPY4 ω-oxidises 14(S)-HDHA to MaR-L1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027042", "summary": "CPY4 ω-oxidises 14(S)-HDHA to MaR-L1 is a Reactome release 97 curated reaction event. The source record connects 14(S)-HDHA, NADPH, O2 to MaR-L1, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "14(S)-HDHA + NADPH + O2 + H+ → MaR-L1 + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-9027029", "reactome_id": "R-ALL-9027029", "label": "14(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:136526", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9027040", "reactome_id": "R-ALL-9027040", "label": "MaR-L1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:136529", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP4 [endoplasmic reticulum membrane]", "activity_id": "9020576", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25200603", "title": "Maresin-like lipid mediators are produced by leukocytes and platelets and rescue reparative function of diabetes-impaired macrophages", "journal": "Chem. Biol.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25200603/" } ], "x": 5670, "y": 2072.2023809523807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027043", "label": "CYPs hydroxylate DHA to 14(R)-HDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027043", "summary": "CYPs hydroxylate DHA to 14(R)-HDHA is a Reactome release 97 curated reaction event. The source record connects DHA, H+, NADPH to 14(R)-HDHA, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "DHA + H+ + NADPH + O2 → 14(R)-HDHA + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-9024011", "reactome_id": "R-ALL-9024011", "label": "DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9027049", "reactome_id": "R-ALL-9027049", "label": "14(R)-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:136525", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A2,2C8,2C9,2D6,2E1,3A4 [endoplasmic reticulum membrane]", "activity_id": "9027048", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25200603", "title": "Maresin-like lipid mediators are produced by leukocytes and platelets and rescue reparative function of diabetes-impaired macrophages", "journal": "Chem. Biol.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25200603/" } ], "x": 5948.666666666667, "y": 2072.2023809523807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027044", "label": "CYP4 ω-oxidises 14(R)-HDHA to MaR-L2", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027044", "summary": "CYP4 ω-oxidises 14(R)-HDHA to MaR-L2 is a Reactome release 97 curated reaction event. The source record connects 14(R)-HDHA, NADPH, O2 to MaR-L2, H2O, NADP+ in cytosol · endoplasmic reticulum membrane.", "equation": "14(R)-HDHA + NADPH + O2 + H+ → MaR-L2 + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-9027049", "reactome_id": "R-ALL-9027049", "label": "14(R)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:136525", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9027045", "reactome_id": "R-ALL-9027045", "label": "MaR-L2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:136530", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP4 [endoplasmic reticulum membrane]", "activity_id": "9020576", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25200603", "title": "Maresin-like lipid mediators are produced by leukocytes and platelets and rescue reparative function of diabetes-impaired macrophages", "journal": "Chem. 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The source record connects 14(S)-HDHA, NADPH, O2 to 14(S),21(S/R)-diHDHA, NADP+, H2O in cytosol · endoplasmic reticulum membrane.", "equation": "14(S)-HDHA + NADPH + O2 + H+ → 14(S),21(S/R)-diHDHA + NADP+ + H2O", "inputs": [ { "node_id": "R-ALL-9027029", "reactome_id": "R-ALL-9027029", "label": "14(S)-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:136526", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9027326", "reactome_id": "R-ALL-9027326", "label": "14(S),21(S/R)-diHDHA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP2E1 [endoplasmic reticulum membrane]", "activity_id": "9028883", "ec_numbers": null, "accessions": [ "P05181" ] } ], "citations": [ { "id": "PMID:19965612", "title": "Novel 14,21-dihydroxy-docosahexaenoic acids: structures, formation pathways, and enhancement of wound healing", "journal": "J. Lipid Res.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19965612/" }, { "id": "PMID:21112969", "title": "14S,21R-dihydroxydocosahexaenoic acid remedies impaired healing and mesenchymal stem cell functions in diabetic wounds", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21112969/" }, { "id": "PMID:21839062", "title": "Autacoid 14S,21R-dihydroxy-docosahexaenoic acid counteracts diabetic impairment of macrophage prohealing functions", "journal": "Am. J. Pathol.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21839062/" } ], "x": 5391.333333333333, "y": 2090.7261904761904, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027321", "label": "CYP2E1 oxidises 14(R)-HDHA to 14(R),21(R)-diHDHA and 14(R),21(S)-diHDHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027321", "summary": "CYP2E1 oxidises 14(R)-HDHA to 14(R),21(R)-diHDHA and 14(R),21(S)-diHDHA is a Reactome release 97 curated reaction event. 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The source record connects DHA, O2 to 7-HDHA in cytosol.", "equation": "DHA + O2 → 7-HDHA", "inputs": [ { "node_id": "R-ALL-9024011", "reactome_id": "R-ALL-9024011", "label": "DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9028274", "reactome_id": "R-ALL-9028274", "label": "7-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140373", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:24736647", "title": "Generation and dietary modulation of anti-inflammatory electrophilic omega-3 fatty acid derivatives", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24736647/" } ], "x": 5948.666666666667, "y": 2248.1785714285716, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027625", "label": "5-HEDH dehydrogenates 7-HDPAn-3 to 7-oxo-DPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027625", "summary": "5-HEDH dehydrogenates 7-HDPAn-3 to 7-oxo-DPAn-3 is a Reactome release 97 curated reaction event. The source record connects 7-HDPAn-3, NADP+ to 7-oxo-DPAn-3, NADPH, H+ in cytosol · endoplasmic reticulum membrane.", "equation": "7-HDPAn-3 + NADP+ → 7-oxo-DPAn-3 + NADPH + H+", "inputs": [ { "node_id": "R-ALL-9028240", "reactome_id": "R-ALL-9028240", "label": "7-HDPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140375", "formula": "C22H33O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9028200", "reactome_id": "R-ALL-9028200", "label": "7-oxo-DPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140386", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of 5-HEDH [endoplasmic reticulum membrane]", "activity_id": "9028228", "ec_numbers": [ "1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:24736647", "title": "Generation and dietary modulation of anti-inflammatory electrophilic omega-3 fatty acid derivatives", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24736647/" }, { "id": "PMID:19164464", "title": "Substrate selectivity of 5-hydroxyeicosanoid dehydrogenase and its inhibition by 5-hydroxy-Delta6-long-chain fatty acids", "journal": "J. Pharmacol. Exp. Ther.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19164464/" } ], "x": 5948.666666666667, "y": 2099.9880952380954, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027627", "label": "Ac-PTGS2 dimer oxidises DHA to 17-HDHA (macrophages)", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027627", "summary": "Ac-PTGS2 dimer oxidises DHA to 17-HDHA (macrophages) is a Reactome release 97 curated reaction event. The source record connects DHA, O2 to 17-HDHA in cytosol · endoplasmic reticulum membrane.", "equation": "DHA + O2 → 17-HDHA", "inputs": [ { "node_id": "R-ALL-9024011", "reactome_id": "R-ALL-9024011", "label": "DHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77016", "formula": "C22H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9027622", "reactome_id": "R-ALL-9027622", "label": "17-HDHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140260", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of Ac-PTGS2 dimer [endoplasmic reticulum membrane]", "activity_id": "2161919", "ec_numbers": [ "1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" }, { "id": "PMID:20436486", "title": "Cyclooxygenase-2 generates anti-inflammatory mediators from omega-3 fatty acids", "journal": "Nat. Chem. Biol.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20436486/" } ], "x": 5391.333333333333, "y": 2257.440476190476, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027628", "label": "ALOX5 oxidises EPA to 5-HEPE", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027628", "summary": "ALOX5 oxidises EPA to 5-HEPE is a Reactome release 97 curated reaction event. The source record connects EPA, O2 to 5-HEPE in cytosol.", "equation": "EPA + O2 → 5-HEPE", "inputs": [ { "node_id": "R-ALL-2026977", "reactome_id": "R-ALL-2026977", "label": "EPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58562", "formula": "C20H29O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9028283", "reactome_id": "R-ALL-9028283", "label": "5-HEPE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90737", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:24736647", "title": "Generation and dietary modulation of anti-inflammatory electrophilic omega-3 fatty acid derivatives", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24736647/" }, { "id": "PMID:8847485", "title": "Formation of a 5-oxo metabolite of 5,8,11,14,17-eicosapentaenoic acid and its effects on human neutrophils and eosinophils", "journal": "J. Lipid Res.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8847485/" } ], "x": 5670, "y": 2257.440476190476, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027631", "label": "5-HEDH dehydrogenates 7-HDHA to 7-oxo-DHA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027631", "summary": "5-HEDH dehydrogenates 7-HDHA to 7-oxo-DHA is a Reactome release 97 curated reaction event. The source record connects 7-HDHA, NADP+ to 7-oxo-DHA, NADPH, H+ in cytosol · endoplasmic reticulum membrane.", "equation": "7-HDHA + NADP+ → 7-oxo-DHA + NADPH + H+", "inputs": [ { "node_id": "R-ALL-9028274", "reactome_id": "R-ALL-9028274", "label": "7-HDHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140373", "formula": "C22H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9028265", "reactome_id": "R-ALL-9028265", "label": "7-oxo-DHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140382", "formula": "C22H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of 5-HEDH [endoplasmic reticulum membrane]", "activity_id": "9028228", "ec_numbers": [ "1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:24736647", "title": "Generation and dietary modulation of anti-inflammatory electrophilic omega-3 fatty acid derivatives", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24736647/" }, { "id": "PMID:19164464", "title": "Substrate selectivity of 5-hydroxyeicosanoid dehydrogenase and its inhibition by 5-hydroxy-Delta6-long-chain fatty acids", "journal": "J. Pharmacol. Exp. Ther.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19164464/" } ], "x": 5391.333333333333, "y": 2109.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027632", "label": "5-HEDH dehydrogenates 5-HEPE to 5-oxo-EPA", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027632", "summary": "5-HEDH dehydrogenates 5-HEPE to 5-oxo-EPA is a Reactome release 97 curated reaction event. The source record connects 5-HEPE, NADP+ to 5-oxo-EPA, NADPH, H+ in cytosol · endoplasmic reticulum membrane.", "equation": "5-HEPE + NADP+ → 5-oxo-EPA + NADPH + H+", "inputs": [ { "node_id": "R-ALL-9028283", "reactome_id": "R-ALL-9028283", "label": "5-HEPE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90737", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9028202", "reactome_id": "R-ALL-9028202", "label": "5-oxo-EPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140337", "formula": "C20H27O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor of 5-HEDH [endoplasmic reticulum membrane]", "activity_id": "9028228", "ec_numbers": [ "1.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:8847485", "title": "Formation of a 5-oxo metabolite of 5,8,11,14,17-eicosapentaenoic acid and its effects on human neutrophils and eosinophils", "journal": "J. Lipid Res.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8847485/" }, { "id": "PMID:24736647", "title": "Generation and dietary modulation of anti-inflammatory electrophilic omega-3 fatty acid derivatives", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24736647/" }, { "id": "PMID:19164464", "title": "Substrate selectivity of 5-hydroxyeicosanoid dehydrogenase and its inhibition by 5-hydroxy-Delta6-long-chain fatty acids", "journal": "J. Pharmacol. Exp. Ther.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19164464/" } ], "x": 5670, "y": 2109.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9027633", "label": "ALOX5 oxidises DPAn-3 to 7-HDPAn-3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9027633", "summary": "ALOX5 oxidises DPAn-3 to 7-HDPAn-3 is a Reactome release 97 curated reaction event. The source record connects O2, DPAn-3 to 7-HDPAn-3 in cytosol.", "equation": "O2 + DPAn-3 → 7-HDPAn-3", "inputs": [ { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9608444", "reactome_id": "R-ALL-9608444", "label": "DPAn-3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77224", "formula": "C22H33O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9028240", "reactome_id": "R-ALL-9028240", "label": "7-HDPAn-3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140375", "formula": "C22H33O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of ALOX5 [cytosol]", "activity_id": "9020578", "ec_numbers": [ "1.13" ], "accessions": [ "P09917" ] } ], "citations": [ { "id": "PMID:24736647", "title": "Generation and dietary modulation of anti-inflammatory electrophilic omega-3 fatty acid derivatives", "journal": "PLoS ONE", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24736647/" } ], "x": 5948.666666666667, "y": 2257.440476190476, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9028255", "label": "PTGS2 dimer oxidises EPA to PGH3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9028255", "summary": "PTGS2 dimer oxidises EPA to PGH3 is a Reactome release 97 curated reaction event. The source record connects EPA, O2 to PGH3 in cytosol · endoplasmic reticulum membrane.", "equation": "EPA + O2 → PGH3", "inputs": [ { "node_id": "R-ALL-2026977", "reactome_id": "R-ALL-2026977", "label": "EPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58562", "formula": "C20H29O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9028280", "reactome_id": "R-ALL-9028280", "label": "PGH3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133134", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen of PTGS2 dimer [endoplasmic reticulum membrane]", "activity_id": "9027536", "ec_numbers": [ "1.13" ], "accessions": [] } ], "citations": [ { "id": "PMID:20436486", "title": "Cyclooxygenase-2 generates anti-inflammatory mediators from omega-3 fatty acids", "journal": "Nat. Chem. Biol.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20436486/" }, { "id": "PMID:12391014", "title": "Resolvins: a family of bioactive products of omega-3 fatty acid transformation circuits initiated by aspirin treatment that counter proinflammation signals", "journal": "J. Exp. Med.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12391014/" }, { "id": "PMID:17519235", "title": "Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products", "journal": "J. Biol. Chem.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17519235/" } ], "x": 5391.333333333333, "y": 2266.7023809523807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9028260", "label": "δ12-PGJ3 spontaneously dehydrates to 15d-PGJ3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9028260", "summary": "δ12-PGJ3 spontaneously dehydrates to 15d-PGJ3 is a Reactome release 97 curated reaction event. The source record connects δ12-PGJ3 to 15d-PGJ3, H2O in cytosol.", "equation": "δ12-PGJ3 → 15d-PGJ3 + H2O", "inputs": [ { "node_id": "R-ALL-9028201", "reactome_id": "R-ALL-9028201", "label": "δ12-PGJ3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140288", "formula": "C20H27O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9028207", "reactome_id": "R-ALL-9028207", "label": "15d-PGJ3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140283", "formula": "C20H25O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:6578214", "title": "Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro", "journal": "J. Biol. Chem.", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6578214/" } ], "x": 5391.333333333333, "y": 2331.535714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9028263", "label": "PGJ3 isomerises to δ12-PGJ3", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9028263", "summary": "PGJ3 isomerises to δ12-PGJ3 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PGJ3 to δ12-PGJ3 in cytosol.", "equation": "PGJ3 → δ12-PGJ3", "inputs": [ { "node_id": "R-ALL-9028230", "reactome_id": "R-ALL-9028230", "label": "PGJ3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140285", "formula": "C20H27O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9028201", "reactome_id": "R-ALL-9028201", "label": "δ12-PGJ3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140288", "formula": "C20H27O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:6578214", "title": "Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro", "journal": "J. Biol. Chem.", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6578214/" } ], "x": 5391.333333333333, "y": 2350.059523809524, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9028273", "label": "PGH3 spontaneously dehydrates to PGJ3", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9028273", "summary": "PGH3 spontaneously dehydrates to PGJ3 is a Reactome release 97 curated reaction event. The source record connects PGH3 to PGJ3, H2O in cytosol.", "equation": "PGH3 → PGJ3 + H2O", "inputs": [ { "node_id": "R-ALL-9028280", "reactome_id": "R-ALL-9028280", "label": "PGH3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:133134", "formula": "C20H29O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9028230", "reactome_id": "R-ALL-9028230", "label": "PGJ3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140285", "formula": "C20H27O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:6578214", "title": "Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro", "journal": "J. Biol. Chem.", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6578214/" } ], "x": 5670, "y": 2331.535714285714, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9031802", "label": "Maresins translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9031802", "summary": "Maresins translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Maresins to Maresins in cytosol · extracellular region.", "equation": "Maresins → Maresins", "inputs": [ { "node_id": "R-ALL-9031813", "reactome_id": "R-ALL-9031813", "label": "Maresins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9031815", "reactome_id": "R-ALL-9031815", "label": "Maresins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:25139562", "title": "Protectins and maresins: New pro-resolving families of mediators in acute inflammation and resolution bioactive metabolome", "journal": "Biochim. Biophys. Acta", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25139562/" } ], "x": 5391.333333333333, "y": 2368.5833333333335, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9031803", "label": "Maresin-like SPMs translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9031803", "summary": "Maresin-like SPMs translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Maresin-like SPMs to Maresin-like SPMs in cytosol · extracellular region.", "equation": "Maresin-like SPMs → Maresin-like SPMs", "inputs": [ { "node_id": "R-ALL-9031800", "reactome_id": "R-ALL-9031800", "label": "Maresin-like SPMs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9031809", "reactome_id": "R-ALL-9031809", "label": "Maresin-like SPMs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:25200603", "title": "Maresin-like lipid mediators are produced by leukocytes and platelets and rescue reparative function of diabetes-impaired macrophages", "journal": "Chem. Biol.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25200603/" } ], "x": 5670, "y": 2368.5833333333335, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9031807", "label": "Protectins translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9031807", "summary": "Protectins translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Protectins to Protectins in cytosol · extracellular region.", "equation": "Protectins → Protectins", "inputs": [ { "node_id": "R-ALL-9031812", "reactome_id": "R-ALL-9031812", "label": "Protectins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9031819", "reactome_id": "R-ALL-9031819", "label": "Protectins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:25359497", "title": "Lipid mediators in the resolution of inflammation", "journal": "Cold Spring Harb Perspect Biol", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25359497/" }, { "id": "PMID:25139562", "title": "Protectins and maresins: New pro-resolving families of mediators in acute inflammation and resolution bioactive metabolome", "journal": "Biochim. Biophys. Acta", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25139562/" } ], "x": 5948.666666666667, "y": 2368.5833333333335, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9031856", "label": "Sulfido conjugates translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9031856", "summary": "Sulfido conjugates translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects sulfido conjugates to sulfido conjugates in cytosol · extracellular region.", "equation": "sulfido conjugates → sulfido conjugates", "inputs": [ { "node_id": "R-ALL-9031855", "reactome_id": "R-ALL-9031855", "label": "sulfido conjugates", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9031853", "reactome_id": "R-ALL-9031853", "label": "sulfido conjugates", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:25713027", "title": "Novel proresolving and tissue-regenerative resolvin and protectin sulfido-conjugated pathways", "journal": "FASEB J.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25713027/" }, { "id": "PMID:27791009", "title": "Maresin conjugates in tissue regeneration biosynthesis enzymes in human macrophages", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27791009/" } ], "x": 5391.333333333333, "y": 2377.845238095238, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9031881", "label": "DPAn-3 resolvins translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9031881", "summary": "DPAn-3 resolvins translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DPAn-3 resolvins to DPAn-3 resolvins in cytosol · extracellular region.", "equation": "DPAn-3 resolvins → DPAn-3 resolvins", "inputs": [ { "node_id": "R-ALL-9031889", "reactome_id": "R-ALL-9031889", "label": "DPAn-3 resolvins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9031890", "reactome_id": "R-ALL-9031890", "label": "DPAn-3 resolvins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" }, { "id": "PMID:28408222", "title": "Recent advances in the chemistry and biology of anti-inflammatory and specialized pro-resolving mediators biosynthesized from n-3 docosapentaenoic acid", "journal": "Bioorg. Med. Chem. Lett.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28408222/" } ], "x": 5670, "y": 2377.845238095238, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9031884", "label": "DPAn-6 SPMs translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9031884", "summary": "DPAn-6 SPMs translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DPAn-6 SPMs to DPAn-6 SPMs in cytosol · extracellular region.", "equation": "DPAn-6 SPMs → DPAn-6 SPMs", "inputs": [ { "node_id": "R-ALL-9031904", "reactome_id": "R-ALL-9031904", "label": "DPAn-6 SPMs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9031892", "reactome_id": "R-ALL-9031892", "label": "DPAn-6 SPMs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19679107", "title": "Metabolism and biological production of resolvins derived from docosapentaenoic acid (DPAn-6)", "journal": "Biochem. Pharmacol.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19679107/" } ], "x": 5948.666666666667, "y": 2377.845238095238, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9031894", "label": "DPAn-3 maresins translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9031894", "summary": "DPAn-3 maresins translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects DPAn-3 maresins to DPAn-3 maresins in cytosol · extracellular region.", "equation": "DPAn-3 maresins → DPAn-3 maresins", "inputs": [ { "node_id": "R-ALL-9031885", "reactome_id": "R-ALL-9031885", "label": "DPAn-3 maresins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9031900", "reactome_id": "R-ALL-9031900", "label": "DPAn-3 maresins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:28408222", "title": "Recent advances in the chemistry and biology of anti-inflammatory and specialized pro-resolving mediators biosynthesized from n-3 docosapentaenoic acid", "journal": "Bioorg. Med. Chem. Lett.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28408222/" }, { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" } ], "x": 5391.333333333333, "y": 2387.107142857143, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9031896", "label": "PD1n-3, PD2n-3 translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9031896", "summary": "PD1n-3, PD2n-3 translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PD1n-3DPA,PD2n-3DPA to PD1n-3DPA,PD2n-3DPA in cytosol · extracellular region.", "equation": "PD1n-3DPA,PD2n-3DPA → PD1n-3DPA,PD2n-3DPA", "inputs": [ { "node_id": "R-ALL-9026023", "reactome_id": "R-ALL-9026023", "label": "PD1n-3DPA,PD2n-3DPA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9031899", "reactome_id": "R-ALL-9031899", "label": "PD1n-3DPA,PD2n-3DPA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:28408222", "title": "Recent advances in the chemistry and biology of anti-inflammatory and specialized pro-resolving mediators biosynthesized from n-3 docosapentaenoic acid", "journal": "Bioorg. Med. Chem. Lett.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28408222/" }, { "id": "PMID:23736886", "title": "Novel n-3 immunoresolvents: structures and actions", "journal": "Sci Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23736886/" } ], "x": 5670, "y": 2387.107142857143, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9032021", "label": "RvT1-4 translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9032021", "summary": "RvT1-4 translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects RvT1-4 to RvT1-4 in cytosol · extracellular region.", "equation": "RvT1-4 → RvT1-4", "inputs": [ { "node_id": "R-ALL-9026394", "reactome_id": "R-ALL-9026394", "label": "RvT1-4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9032020", "reactome_id": "R-ALL-9032020", "label": "RvT1-4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:28465323", "title": "13-Series resolvins mediate the leukocyte-platelet actions of atorvastatin and pravastatin in inflammatory arthritis", "journal": "FASEB J.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28465323/" }, { "id": "PMID:26236990", "title": "Elucidation of novel 13-series resolvins that increase with atorvastatin and clear infections", "journal": "Nat. Med.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26236990/" } ], "x": 5948.666666666667, "y": 2387.107142857143, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9032036", "label": "LTA4 translocates from neutrophil cytosol to platelet cytosol", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9032036", "summary": "LTA4 translocates from neutrophil cytosol to platelet cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects LTA4 to LTA4 in cytosol.", "equation": "LTA4 → LTA4", "inputs": [ { "node_id": "R-ALL-265281", "reactome_id": "R-ALL-265281", "label": "LTA4", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9032038", "reactome_id": "R-ALL-9032038", "label": "LTA4", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:2820055", "title": "Leukotrienes and lipoxins: structures, biosynthesis, and biological effects", "journal": "Science", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2820055/" } ], "x": 5670, "y": 2350.059523809524, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9032054", "label": "LXA4/B4 translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9032054", "summary": "LXA4/B4 translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects LXA4/B4 to LXA4/B4 in cytosol · extracellular region.", "equation": "LXA4/B4 → LXA4/B4", "inputs": [ { "node_id": "R-ALL-2161856", "reactome_id": "R-ALL-2161856", "label": "LXA4/B4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9037644", "reactome_id": "R-ALL-9037644", "label": "LXA4/B4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:2820055", "title": "Leukotrienes and lipoxins: structures, biosynthesis, and biological effects", "journal": "Science", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2820055/" } ], "x": 5391.333333333333, "y": 2396.3690476190477, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9032315", "label": "oxo-DHAs translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9032315", "summary": "oxo-DHAs translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects oxo-DHAs to oxo-DHAs in cytosol · extracellular region.", "equation": "oxo-DHAs → oxo-DHAs", "inputs": [ { "node_id": "R-ALL-9032319", "reactome_id": "R-ALL-9032319", "label": "oxo-DHAs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9032322", "reactome_id": "R-ALL-9032322", "label": "oxo-DHAs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "fatty acid transmembrane transporter activity of transporter [plasma membrane]", "activity_id": "9911705", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:26339618", "title": "Endogenous Generation and Signaling Actions of Omega-3 Fatty Acid Electrophilic Derivatives", "journal": "Biomed Res Int", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26339618/" } ], "x": 5391.333333333333, "y": 2340.7976190476193, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9032323", "label": "oxo-DPAn-3s translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9032323", "summary": "oxo-DPAn-3s translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects oxo-DPAn-3s to oxo-DPAn-3s in cytosol · extracellular region.", "equation": "oxo-DPAn-3s → oxo-DPAn-3s", "inputs": [ { "node_id": "R-ALL-9032318", "reactome_id": "R-ALL-9032318", "label": "oxo-DPAn-3s", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9032325", "reactome_id": "R-ALL-9032325", "label": "oxo-DPAn-3s", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "fatty acid transmembrane transporter activity of transporter [plasma membrane]", "activity_id": "9911705", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:26339618", "title": "Endogenous Generation and Signaling Actions of Omega-3 Fatty Acid Electrophilic Derivatives", "journal": "Biomed Res Int", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26339618/" } ], "x": 5670, "y": 2340.7976190476193, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9032327", "label": "5-oxo-EPA, 15d-PGJ3 translocate from cytosol to extracellular region", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9032327", "summary": "5-oxo-EPA, 15d-PGJ3 translocate from cytosol to extracellular region is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 5-oxo-EPA, 15d-PGJ3 to 5-oxo-EPA, 15d-PGJ3 in cytosol · extracellular region.", "equation": "5-oxo-EPA + 15d-PGJ3 → 5-oxo-EPA + 15d-PGJ3", "inputs": [ { "node_id": "R-ALL-9028202", "reactome_id": "R-ALL-9028202", "label": "5-oxo-EPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140337", "formula": "C20H27O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9028207", "reactome_id": "R-ALL-9028207", "label": "15d-PGJ3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140283", "formula": "C20H25O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9032328", "reactome_id": "R-ALL-9032328", "label": "5-oxo-EPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140337", "formula": "C20H27O3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9032313", "reactome_id": "R-ALL-9032313", "label": "15d-PGJ3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140283", "formula": "C20H25O3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "prostaglandin transmembrane transporter activity of transporter [plasma membrane]", "activity_id": "9911740", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:26339618", "title": "Endogenous Generation and Signaling Actions of Omega-3 Fatty Acid Electrophilic Derivatives", "journal": "Biomed Res Int", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26339618/" } ], "x": 5948.666666666667, "y": 2331.535714285714, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9034539", "label": "PXLP-K56-SRR dimer deaminates L-Ser", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "serine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19170" ], "source_url": "https://reactome.org/content/detail/R-HSA-9034539", "summary": "PXLP-K56-SRR dimer deaminates L-Ser is a Reactome release 97 curated reaction event. The source record connects L-Ser to PYR, NH4+ in cytosol and cross-references RHEA:19170.", "equation": "L-Ser → PYR + NH4+", "inputs": [ { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "D-serine ammonia-lyase activity of PXLP-K56-SRR dimer [cytosol]", "activity_id": "9014746", "ec_numbers": [ "4.3.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:24795622", "title": "Serine racemase: a key player in apoptosis and necrosis", "journal": "Front Synaptic Neurosci", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24795622/" }, { "id": "PMID:12393813", "title": "Cofactors of serine racemase that physiologically stimulate the synthesis of the N-methyl-D-aspartate (NMDA) receptor coagonist D-serine", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12393813/" }, { "id": "PMID:15536068", "title": "Serine racemase modulates intracellular D-serine levels through an alpha,beta-elimination activity", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15536068/" }, { "id": "PMID:20106978", "title": "The structure of mammalian serine racemase: evidence for conformational changes upon inhibitor binding", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20106978/" } ], "x": 590, "y": 2917.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9034756", "label": "MPST transfers sulfur atom from 3MPYR to form CysS248-MPST", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:56297" ], "source_url": "https://reactome.org/content/detail/R-HSA-9034756", "summary": "MPST transfers sulfur atom from 3MPYR to form CysS248-MPST is a Reactome release 97 curated reaction event. The source record connects 3MPYR, MPST to CysS248-MPST, PYR in mitochondrial matrix and cross-references RHEA:56297.", "equation": "3MPYR + MPST → CysS248-MPST + PYR", "inputs": [ { "node_id": "R-ALL-9012592", "reactome_id": "R-ALL-9012592", "label": "3MPYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57678", "formula": "C3H3O3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9012720", "reactome_id": "R-HSA-9012720", "label": "MPST", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9034913", "reactome_id": "R-HSA-9034913", "label": "CysS248-MPST", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfurtransferase activity of MPST [mitochondrial matrix]", "activity_id": "9012723", "ec_numbers": [ "2.8.1" ], "accessions": [ "P25325" ] } ], "citations": [ { "id": "PMID:23413359", "title": "Polysulfides are possible H2S-derived signaling molecules in rat brain", "journal": "FASEB J.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23413359/" }, { "id": "PMID:26437775", "title": "Identification of H2S3 and H2S produced by 3-mercaptopyruvate sulfurtransferase in the brain", "journal": "Sci Rep", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26437775/" }, { "id": "PMID:29055825", "title": "Analysis of endogenous H2S and H2Sn in mouse brain by high-performance liquid chromatography with fluorescence and tandem mass spectrometric detection", "journal": "Free Radic. Biol. Med.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/29055825/" }, { "id": "PMID:27484215", "title": "Hydrogen polysulfide (H2S n ) signaling along with hydrogen sulfide (H2S) and nitric oxide (NO)", "journal": "J Neural Transm (Vienna)", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27484215/" } ], "x": 1808.6666666666667, "y": 2965.4444444444443, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9035484", "label": "MPST and TXN2 enable the synthesis of disulfanide from CysS248-MPST", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "sulfur-amino-acids" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:56301" ], "source_url": "https://reactome.org/content/detail/R-HSA-9035484", "summary": "MPST and TXN2 enable the synthesis of disulfanide from CysS248-MPST is a Reactome release 97 curated reaction event. The source record connects TXN2, CysS248-MPST to MPST, 2xHC-TXN2, disulfanide in mitochondrial matrix and cross-references RHEA:56301.", "equation": "TXN2 + CysS248-MPST → MPST + 2xHC-TXN2 + disulfanide", "inputs": [ { "node_id": "R-HSA-3323048", "reactome_id": "R-HSA-3323048", "label": "TXN2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9034913", "reactome_id": "R-HSA-9034913", "label": "CysS248-MPST", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9012720", "reactome_id": "R-HSA-9012720", "label": "MPST", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-3323059", "reactome_id": "R-HSA-3323059", "label": "2xHC-TXN2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9930114", "reactome_id": "R-ALL-9930114", "label": "disulfanide", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30604", "formula": "HS2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor of CysS248-MPST:TXN2 [mitochondrial matrix]", "activity_id": "9930120", "ec_numbers": [ "1.8.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:23413359", "title": "Polysulfides are possible H2S-derived signaling molecules in rat brain", "journal": "FASEB J.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23413359/" }, { "id": "PMID:28874874", "title": "3-Mercaptopyruvate sulfurtransferase produces potential redox regulators cysteine- and glutathione-persulfide (Cys-SSH and GSSH) together with signaling molecules H2S2, H2S3 and H2S", "journal": "Sci Rep", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28874874/" }, { "id": "PMID:29055825", "title": "Analysis of endogenous H2S and H2Sn in mouse brain by high-performance liquid chromatography with fluorescence and tandem mass spectrometric detection", "journal": "Free Radic. Biol. Med.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/29055825/" }, { "id": "PMID:16195549", "title": "Crystal structures of oxidized and reduced forms of human mitochondrial thioredoxin 2", "journal": "Protein Sci.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16195549/" }, { "id": "PMID:23698001", "title": "Structure and kinetic analysis of H2S production by human mercaptopyruvate sulfurtransferase", "journal": "J. Biol. Chem.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23698001/" } ], "x": 1251.3333333333333, "y": 2984.5555555555557, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9036727", "label": "GAA hydrolyzes lysosomal glycogen", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9036727", "summary": "GAA hydrolyzes lysosomal glycogen is a Reactome release 97 curated reaction event. The source record connects glycogen-GYG1 dimer, H2O to poly((1,4)-alpha-glucosyl)GYG1 dimer, Glc in lysosomal lumen.", "equation": "glycogen-GYG1 dimer + 2 H2O → poly((1,4)-alpha-glucosyl)GYG1 dimer + 2 Glc", "inputs": [ { "node_id": "R-HSA-9036726", "reactome_id": "R-HSA-9036726", "label": "glycogen-GYG1 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-5357533", "reactome_id": "R-HSA-5357533", "label": "poly((1,4)-alpha-glucosyl)GYG1 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-1605745", "reactome_id": "R-ALL-1605745", "label": "Glc", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:4167", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "alpha-glucosidase activity of GAA (70, 76 kDa) [lysosomal lumen]", "activity_id": "5357535", "ec_numbers": [ "3.2.1.20" ], "accessions": [] } ], "citations": [ { "id": "PMID:5264799", "title": "Simultaneous absence of alpha-1,4-glucosidase and alpha-1,6-glucosidase activities (pH 4) in tissues of children with type II glycogen storage disease", "journal": "Biochemistry", "year": 1970, "url": "https://pubmed.ncbi.nlm.nih.gov/5264799/" }, { "id": "PMID:3049072", "title": "Primary structure and processing of lysosomal alpha-glucosidase; homology with the intestinal sucrase-isomaltase complex", "journal": "EMBO J.", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3049072/" }, { "id": "PMID:21767668", "title": "Specific functions of lysosomal proteases in endocytic and autophagic pathways", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/21767668/" }, { "id": "PMID:8420990", "title": "Structural and functional changes of lysosomal acid alpha-glucosidase during intracellular transport and maturation", "journal": "J. Biol. Chem.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8420990/" } ], "x": 1530, "y": 966.4230769230769, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9037761", "label": "CYP1, CYP2 hydroxylate (N)PD1 to 22-OH-(N)PD1", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9037761", "summary": "CYP1, CYP2 hydroxylate (N)PD1 to 22-OH-(N)PD1 is a Reactome release 97 curated reaction event. The source record connects (N)PD1, H+, NADPH to 22-OH-(N)PD1, H2O, NADP+ in endoplasmic reticulum membrane · cytosol.", "equation": "(N)PD1 + H+ + NADPH + O2 → 22-OH-(N)PD1 + H2O + NADP+", "inputs": [ { "node_id": "R-ALL-9024886", "reactome_id": "R-ALL-9024886", "label": "(N)PD1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:195348", "formula": "C22H31O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9037755", "reactome_id": "R-ALL-9037755", "label": "22-OH-(N)PD1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140348", "formula": "C22H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of CYP1A1, CYP1A2 [endoplasmic reticulum membrane]", "activity_id": "9037765", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25247845", "title": "Synthesis and anti-inflammatory and pro-resolving activities of 22-OH-PD1, a monohydroxylated metabolite of protectin D1", "journal": "J. Nat. Prod.", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25247845/" }, { "id": "PMID:23956430", "title": "Contributions of the three CYP1 monooxygenases to pro-inflammatory and inflammation-resolution lipid mediator pathways", "journal": "J. Immunol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23956430/" } ], "x": 5670, "y": 2081.464285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-904864", "label": "Mitochondrial AGXT2 tetramer transaminates glyoxylate and alanine to glycine and pyruvate", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "glycine-glyoxylate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:24249" ], "source_url": "https://reactome.org/content/detail/R-HSA-904864", "summary": "Mitochondrial AGXT2 tetramer transaminates glyoxylate and alanine to glycine and pyruvate is a Reactome release 97 curated reaction event. The source record connects L-Ala, glyoxylate to Gly, PYR in mitochondrial matrix and cross-references RHEA:24249.", "equation": "L-Ala + glyoxylate → Gly + PYR", "inputs": [ { "node_id": "R-ALL-379697", "reactome_id": "R-ALL-379697", "label": "L-Ala", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-904849", "reactome_id": "R-ALL-904849", "label": "glyoxylate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-alanine:glyoxylate transaminase activity of AGXT2 tetramer [mitochondrial matrix]", "activity_id": "904847", "ec_numbers": [ "2.6.1.44" ], "accessions": [] } ], "citations": [ { "id": "PMID:20018850", "title": "Human alanine-glyoxylate aminotransferase 2 lowers asymmetric dimethylarginine and protects from inhibition of nitric oxide production", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20018850/" }, { "id": "PMID:2158891", "title": "Purification, characterization and inhibition of D-3-aminoisobutyrate aminotransferase from the rat liver", "journal": "Eur J Biochem", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2158891/" } ], "x": 311.33333333333337, "y": 3237, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-909755", "label": "Mitochondrial uptake of (R)-3-aminoisobutyric acid", "schema_class": "BlackBoxEvent", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-909755", "summary": "Mitochondrial uptake of (R)-3-aminoisobutyric acid is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 3AIB to 3AIB in cytosol · mitochondrial matrix.", "equation": "3AIB → 3AIB", "inputs": [ { "node_id": "R-ALL-73619", "reactome_id": "R-ALL-73619", "label": "3AIB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57731", "formula": "C4H9NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-909779", "reactome_id": "R-ALL-909779", "label": "3AIB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57731", "formula": "C4H9NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10989446", "title": "Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver", "journal": "Methods Enzymol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10989446/" } ], "x": 2938.666666666667, "y": 3181.3214285714284, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-909765", "label": "Mitochondrial uptake of beta-alanine", "schema_class": "BlackBoxEvent", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-909765", "summary": "Mitochondrial uptake of beta-alanine is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects b-Ala to b-Ala in cytosol · mitochondrial matrix.", "equation": "b-Ala → b-Ala", "inputs": [ { "node_id": "R-ALL-73590", "reactome_id": "R-ALL-73590", "label": "b-Ala", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57966", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-909777", "reactome_id": "R-ALL-909777", "label": "b-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57966", "formula": "C3H7NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10989446", "title": "Purification, properties, and sequencing of aminoisobutyrate aminotransferases from rat liver", "journal": "Methods Enzymol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10989446/" } ], "x": 2381.3333333333335, "y": 3188.440476190476, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-909776", "label": "beta-alanine + pyruvate =\u003e 3-oxopropanoate + alanine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:14079" ], "source_url": "https://reactome.org/content/detail/R-HSA-909776", "summary": "beta-alanine + pyruvate =\u003e 3-oxopropanoate + alanine is a Reactome release 97 curated reaction event. The source record connects b-Ala, PYR to OPROP, L-Ala in mitochondrial matrix and cross-references RHEA:14079.", "equation": "b-Ala + PYR → OPROP + L-Ala", "inputs": [ { "node_id": "R-ALL-909777", "reactome_id": "R-ALL-909777", "label": "b-Ala", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57966", "formula": "C3H7NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-909783", "reactome_id": "R-ALL-909783", "label": "OPROP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33190", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-379697", "reactome_id": "R-ALL-379697", "label": "L-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-alanine:pyruvate transaminase activity of AGXT2 tetramer [mitochondrial matrix]", "activity_id": "909784", "ec_numbers": [ "2.6.1.18" ], "accessions": [] } ], "citations": [ { "id": "PMID:20018850", "title": "Human alanine-glyoxylate aminotransferase 2 lowers asymmetric dimethylarginine and protects from inhibition of nitric oxide production", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20018850/" } ], "x": 2938.666666666667, "y": 3167.0833333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-909780", "label": "(R)-3-amino-2-methylpropanoate + pyruvate =\u003e 2-methyl-3-oxopropanoate + L-alanine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "pyrimidine-catabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18394" ], "source_url": "https://reactome.org/content/detail/R-HSA-909780", "summary": "(R)-3-amino-2-methylpropanoate + pyruvate =\u003e 2-methyl-3-oxopropanoate + L-alanine is a Reactome release 97 curated reaction event. The source record connects 3AIB, PYR to L-Ala, 2-methyl-3-oxopropanoate in mitochondrial matrix and cross-references RHEA:18394.", "equation": "3AIB + PYR → L-Ala + 2-methyl-3-oxopropanoate", "inputs": [ { "node_id": "R-ALL-909779", "reactome_id": "R-ALL-909779", "label": "3AIB", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57731", "formula": "C4H9NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-379697", "reactome_id": "R-ALL-379697", "label": "L-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9976511", "reactome_id": "R-ALL-9976511", "label": "2-methyl-3-oxopropanoate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57700", "formula": "C4H5O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(R)-3-amino-2-methylpropionate:pyruvate transaminase activity of AGXT2 tetramer [mitochondrial matrix]", "activity_id": "909768", "ec_numbers": [ "2.6.1.40" ], "accessions": [] } ], "citations": [ { "id": "PMID:20018850", "title": "Human alanine-glyoxylate aminotransferase 2 lowers asymmetric dimethylarginine and protects from inhibition of nitric oxide production", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20018850/" } ], "x": 2660, "y": 3181.3214285714284, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-917892", "label": "FLVCR1-1 transports heme from cytosol to extracellular region", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:75443" ], "source_url": "https://reactome.org/content/detail/R-HSA-917892", "summary": "FLVCR1-1 transports heme from cytosol to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ferroheme to heme in plasma membrane and cross-references RHEA:75443.", "equation": "ferroheme → heme", "inputs": [ { "node_id": "R-ALL-71185", "reactome_id": "R-ALL-71185", "label": "ferroheme", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60344", "formula": "C34H30FeN4O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-917877", "reactome_id": "R-ALL-917877", "label": "heme", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60344", "formula": "C34H30FeN4O4", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "heme transmembrane transporter activity of FLVCR1-1 [plasma membrane]", "activity_id": "917815", "ec_numbers": null, "accessions": [ "Q9Y5Y0" ] } ], "citations": [ { "id": "PMID:15369674", "title": "Identification of a human heme exporter that is essential for erythropoiesis", "journal": "Cell", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15369674/" }, { "id": "PMID:18815190", "title": "Enhanced alternative splicing of the FLVCR1 gene in Diamond Blackfan anemia disrupts FLVCR1 expression and function that are critical for erythropoiesis", "journal": "Haematologica", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18815190/" } ], "x": 6521.333333333333, "y": 2928.035714285714, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-917979", "label": "ABCG2 tetramer transports heme from cytosol to extracellular region", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:19261" ], "source_url": "https://reactome.org/content/detail/R-HSA-917979", "summary": "ABCG2 tetramer transports heme from cytosol to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ferroheme, ATP, H2O to heme, ADP, Pi in plasma membrane and cross-references RHEA:19261.", "equation": "ferroheme + ATP + H2O → heme + ADP + Pi + H+", "inputs": [ { "node_id": "R-ALL-71185", "reactome_id": "R-ALL-71185", "label": "ferroheme", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60344", "formula": "C34H30FeN4O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-917877", "reactome_id": "R-ALL-917877", "label": "heme", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60344", "formula": "C34H30FeN4O4", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "ABC-type heme transporter activity of ABCG2 tetramer [plasma membrane]", "activity_id": "917884", "ec_numbers": [ "7.6.2.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:15001581", "title": "Characterization of oligomeric human half-ABC transporter ATP-binding cassette G2", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15001581/" }, { "id": "PMID:15044468", "title": "The stem cell marker Bcrp/ABCG2 enhances hypoxic cell survival through interactions with heme", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15044468/" }, { "id": "PMID:14576842", "title": "Multidrug resistance mediated by the breast cancer resistance protein BCRP (ABCG2)", "journal": "Oncogene", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14576842/" }, { "id": "PMID:12958161", "title": "The expression and functional characterization of ABCG2 in brain endothelial cells and vessels", "journal": "FASEB J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12958161/" } ], "x": 6800, "y": 2816.8928571428573, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-947499", "label": "Exchange of oxygen with sulfur in MoCo", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-947499", "summary": "Exchange of oxygen with sulfur in MoCo is a Reactome release 97 curated reaction event. The source record connects L-Cys, MoCo to L-Ala, sulfurated MoCo in cytosol.", "equation": "L-Cys + MoCo → L-Ala + sulfurated MoCo", "inputs": [ { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-947539", "reactome_id": "R-ALL-947539", "label": "MoCo", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:25372", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29432", "reactome_id": "R-ALL-29432", "label": "L-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-947575", "reactome_id": "R-ALL-947575", "label": "sulfurated MoCo", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60102", "formula": "C10H11MoN5O8PS3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "molybdenum cofactor sulfurtransferase activity of PXLP-K264-MOCOS [cytosol]", "activity_id": "947545", "ec_numbers": [ "2.8.1.9" ], "accessions": [ "Q96EN8" ] } ], "citations": [ { "id": "PMID:11302742", "title": "Mutation of human molybdenum cofactor sulfurase gene is responsible for classical xanthinuria type II", "journal": "Biochem Biophys Res Commun", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11302742/" } ], "x": 5948.666666666667, "y": 3074.2763157894738, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-947514", "label": "PXLP-K198-NFS1 transfers sulfur from cysteine onto MOCS3", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-947514", "summary": "PXLP-K198-NFS1 transfers sulfur from cysteine onto MOCS3 is a Reactome release 97 curated reaction event. The source record connects L-Cys, MOCS3:Zn2+ (red.) to L-Ala, MOCS3-S-S(1-):Zn2+ in cytosol.", "equation": "L-Cys + MOCS3:Zn2+ (red.) → L-Ala + MOCS3-S-S(1-):Zn2+", "inputs": [ { "node_id": "R-ALL-111707", "reactome_id": "R-ALL-111707", "label": "L-Cys", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:35235", "formula": "C3H7NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-947543", "reactome_id": "R-HSA-947543", "label": "MOCS3:Zn2+ (red.)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29432", "reactome_id": "R-ALL-29432", "label": "L-Ala", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57972", "formula": "C3H7NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-947584", "reactome_id": "R-HSA-947584", "label": "MOCS3-S-S(1-):Zn2+", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cysteine desulfurase activity of PXLP-K198-NFS1-2 dimer [cytosol]", "activity_id": "947571", "ec_numbers": [ "2.8.1.7" ], "accessions": [] } ], "citations": [ { "id": "PMID:18650437", "title": "A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18650437/" } ], "x": 5391.333333333333, "y": 3115.2236842105262, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-947531", "label": "Molybdenum ion transfer onto molybdopterin", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-947531", "summary": "Molybdenum ion transfer onto molybdopterin is a Reactome release 97 curated reaction event. The source record connects MPT, MoO4(2-), ATP to MoCo, AMP, PPi in cytosol · plasma membrane.", "equation": "MPT + MoO4(2-) + ATP + H2O → MoCo + AMP + PPi", "inputs": [ { "node_id": "R-ALL-947496", "reactome_id": "R-ALL-947496", "label": "MPT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58698", "formula": "C10H11N5O6PS2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-947561", "reactome_id": "R-ALL-947561", "label": "MoO4(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36264", "formula": "MoO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-947539", "reactome_id": "R-ALL-947539", "label": "MoCo", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:25372", "formula": "", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "molybdopterin molybdotransferase activity of 3xGPHN:3xMg2+ [plasma membrane]", "activity_id": "947533", "ec_numbers": [ "2.10.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:9990024", "title": "The neurotransmitter receptor-anchoring protein gephyrin reconstitutes molybdenum cofactor biosynthesis in bacteria, plants, and mammalian cells", "journal": "Proc Natl Acad Sci U S A", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9990024/" } ], "x": 5391.333333333333, "y": 3053.8026315789475, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-947535", "label": "Cyclisation of GTP to precursor Z", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-947535", "summary": "Cyclisation of GTP to precursor Z is a Reactome release 97 curated reaction event. The source record connects GTP, AdoMet, H2O to Precursor Z, PPi, L-Met in cytosol.", "equation": "GTP + AdoMet + H2O → Precursor Z + PPi + L-Met + dADE", "inputs": [ { "node_id": "R-ALL-29438", "reactome_id": "R-ALL-29438", "label": "GTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:37565", "formula": "C10H12N5O14P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-71284", "reactome_id": "R-ALL-71284", "label": "AdoMet", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-947537", "reactome_id": "R-ALL-947537", "label": "Precursor Z", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59648", "formula": "C10H11N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174390", "reactome_id": "R-ALL-174390", "label": "L-Met", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57844", "formula": "C5H11NO2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-947703", "reactome_id": "R-ALL-947703", "label": "dADE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17319", "formula": "C10H13N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "S-adenosyl-L-methionine binding activity of holo-MOCS1 [cytosol]", "activity_id": "947552", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:11891227", "title": "Functionality of alternative splice forms of the first enzymes involved in human molybdenum cofactor biosynthesis", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11891227/" }, { "id": "PMID:15180982", "title": "Characterization of MOCS1A, an oxygen-sensitive iron-sulfur protein involved in human molybdenum cofactor biosynthesis", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15180982/" } ], "x": 5670, "y": 3053.8026315789475, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-947538", "label": "Transfer of sulfur from MOCS3-S-S onto MOCS2A", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-947538", "summary": "Transfer of sulfur from MOCS3-S-S onto MOCS2A is a Reactome release 97 curated reaction event. The source record connects MOCS2, ATP, MOCS3-S-S(1-):Zn2+ to SOG-MOCS2, AMP, PPi in cytosol.", "equation": "MOCS2 + ATP + MOCS3-S-S(1-):Zn2+ → SOG-MOCS2 + AMP + PPi + MOCS3:Zn2+ (ox.)", "inputs": [ { "node_id": "R-HSA-947542", "reactome_id": "R-HSA-947542", "label": "MOCS2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-947584", "reactome_id": "R-HSA-947584", "label": "MOCS3-S-S(1-):Zn2+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-947517", "reactome_id": "R-HSA-947517", "label": "SOG-MOCS2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-947568", "reactome_id": "R-HSA-947568", "label": "MOCS3:Zn2+ (ox.)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "sulfurtransferase activity of MOCS3-S-S(1-):Zn2+ [cytosol]", "activity_id": "947580", "ec_numbers": [ "2.8.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:18650437", "title": "A novel role for human Nfs1 in the cytoplasm: Nfs1 acts as a sulfur donor for MOCS3, a protein involved in molybdenum cofactor biosynthesis", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18650437/" } ], "x": 5948.666666666667, "y": 3084.5131578947367, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-947541", "label": "Sulfhydrylation and ring cleavage of precursor Z", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-947541", "summary": "Sulfhydrylation and ring cleavage of precursor Z is a Reactome release 97 curated reaction event. The source record connects Precursor Z, H2O, 2xMOCS2-CO-S(1-):2xMOCS2B to MPT, H+, 2xMOCS2A:2xMOCS2B in cytosol.", "equation": "Precursor Z + H2O + 2xMOCS2-CO-S(1-):2xMOCS2B → MPT + H+ + 2xMOCS2A:2xMOCS2B", "inputs": [ { "node_id": "R-ALL-947537", "reactome_id": "R-ALL-947537", "label": "Precursor Z", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:59648", "formula": "C10H11N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-947582", "reactome_id": "R-HSA-947582", "label": "2xMOCS2-CO-S(1-):2xMOCS2B", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-947496", "reactome_id": "R-ALL-947496", "label": "MPT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58698", "formula": "C10H11N5O6PS2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-947569", "reactome_id": "R-HSA-947569", "label": "2xMOCS2A:2xMOCS2B", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molybdopterin synthase activity of 2xMOCS2-CO-S(1-):2xMOCS2B [cytosol]", "activity_id": "947528", "ec_numbers": [ "2.8.1.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:12571226", "title": "Mechanistic and mutational studies of Escherichia coli molybdopterin synthase clarify the final step of molybdopterin biosynthesis", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12571226/" }, { "id": "PMID:12732628", "title": "Mechanistic studies of human molybdopterin synthase reaction and characterization of mutants identified in group B patients of molybdenum cofactor deficiency", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12732628/" } ], "x": 5391.333333333333, "y": 3084.5131578947367, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9605496", "label": "NME6 phosphorylates (d)NDPs to (d)NTPs", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-interconversion" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:27687", "RHEA:25238", "RHEA:25099", "RHEA:27675", "RHEA:27691", "RHEA:27679", "RHEA:27683", "RHEA:28583" ], "source_url": "https://reactome.org/content/detail/R-HSA-9605496", "summary": "NME6 phosphorylates (d)NDPs to (d)NTPs is a Reactome release 97 curated reaction event. The source record connects (d)NDP, ATP to (d)NTP, ADP in mitochondrial matrix and cross-references RHEA:27687, RHEA:25238, RHEA:25099, RHEA:27675, RHEA:27691, RHEA:27679, RHEA:27683, RHEA:28583.", "equation": "(d)NDP + ATP → (d)NTP + ADP", "inputs": [ { "node_id": "R-ALL-482803", "reactome_id": "R-ALL-482803", "label": "(d)NDP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113593", "reactome_id": "R-ALL-113593", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-482807", "reactome_id": "R-ALL-482807", "label": "(d)NTP", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "nucleoside diphosphate kinase activity of NME6 [mitochondrial matrix]", "activity_id": "9605498", "ec_numbers": [ "2.7.4.6" ], "accessions": [ "O75414" ] } ], "citations": [ { "id": "PMID:10618642", "title": "A novel human nucleoside diphosphate (NDP) kinase, Nm23-H6, localizes in mitochondria and affects cytokinesis", "journal": "J. Cell. Biochem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10618642/" }, { "id": "PMID:10453732", "title": "NME6: a new member of the nm23/nucleoside diphosphate kinase gene family located on human chromosome 3p21.3", "journal": "Hum. Genet.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10453732/" } ], "x": 3600, "y": 3061.1875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9632033", "label": "CSGALNACTs transfer GalNAc to CS chain", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9632033", "summary": "CSGALNACTs transfer GalNAc to CS chain is a Reactome release 97 curated reaction event. The source record connects chondroitin(2)-core proteins, UDP-GalNAc to UDP, chondroitin(3)-core proteins, H+ in Golgi membrane · Golgi lumen.", "equation": "chondroitin(2)-core proteins + UDP-GalNAc → UDP + chondroitin(3)-core proteins + H+", "inputs": [ { "node_id": "R-HSA-2064050", "reactome_id": "R-HSA-2064050", "label": "chondroitin(2)-core proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-735700", "reactome_id": "R-ALL-735700", "label": "UDP-GalNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57847", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2064075", "reactome_id": "R-HSA-2064075", "label": "chondroitin(3)-core proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity of CSGALNACT [Golgi membrane]", "activity_id": "9632031", "ec_numbers": [ "2.4.1.175" ], "accessions": [] } ], "citations": [ { "id": "PMID:11788602", "title": "Molecular cloning and expression of human chondroitin N-acetylgalactosaminyltransferase: the key enzyme for chain initiation and elongation of chondroitin/dermatan sulfate on the protein linkage region tetrasaccharide shared by heparin/heparan sulfate", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11788602/" }, { "id": "PMID:12163485", "title": "Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that transfers N-acetylgalactosamine to glucuronic acid in initiation and elongation of chondroitin sulfate synthesis", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12163485/" }, { "id": "PMID:27599773", "title": "Chondroitin Sulfate N-acetylgalactosaminyltransferase-1 (CSGalNAcT-1) Deficiency Results in a Mild Skeletal Dysplasia and Joint Laxity", "journal": "Hum Mutat", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/27599773/" }, { "id": "PMID:23774590", "title": "Biosynthesis and function of chondroitin sulfate", "journal": "Biochim Biophys Acta", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23774590/" } ], "x": 2938.666666666667, "y": 1007.2272727272727, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9632038", "label": "UGT1A1 tetramer transfers GlcA from UDP-GlcA to BMG to form BDG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two", "porphyrins" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9632038", "summary": "UGT1A1 tetramer transfers GlcA from UDP-GlcA to BMG to form BDG is a Reactome release 97 curated reaction event. The source record connects BMG, UDP-GlcA to BDG, UDP in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "BMG + UDP-GlcA → BDG + UDP", "inputs": [ { "node_id": "R-ALL-159160", "reactome_id": "R-ALL-159160", "label": "BMG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16427", "formula": "C39H44N4O12", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174384", "reactome_id": "R-ALL-174384", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159149", "reactome_id": "R-ALL-159149", "label": "BDG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58471", "formula": "C45H50N4O18", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158602", "reactome_id": "R-ALL-158602", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucuronosyltransferase activity of UGT1A1 tetramer [endoplasmic reticulum membrane]", "activity_id": "9704997", "ec_numbers": [ "2.4.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:6654901", "title": "The hepatic microsomal formation of bilirubin diglucuronide", "journal": "J Biol Chem", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6654901/" }, { "id": "PMID:6796486", "title": "Bilirubin mono- and diglucuronide formation by human liver in vitro: assay by high-pressure liquid chromatography", "journal": "Hepatology", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/6796486/" }, { "id": "PMID:3082969", "title": "Microsomal UDP-glucuronyltransferase-catalyzed bilirubin diglucuronide formation in human liver", "journal": "J Hepatol", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3082969/" }, { "id": "PMID:4639028", "title": "Bilirubin conjugates in bile of man and rat in the normal state and in liver disease", "journal": "J Clin Invest", "year": 1972, "url": "https://pubmed.ncbi.nlm.nih.gov/4639028/" } ], "x": 6800, "y": 3011.3928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9632039", "label": "UGT1A1 transfers GlcA from UDP-GlcA to BIL to form BMG", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two", "porphyrins" ], "compartment": "endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005788", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9632039", "summary": "UGT1A1 transfers GlcA from UDP-GlcA to BIL to form BMG is a Reactome release 97 curated reaction event. The source record connects BIL, UDP-GlcA to BMG, UDP in endoplasmic reticulum lumen · endoplasmic reticulum membrane.", "equation": "BIL + UDP-GlcA → BMG + UDP", "inputs": [ { "node_id": "R-ALL-159151", "reactome_id": "R-ALL-159151", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-174384", "reactome_id": "R-ALL-174384", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-159160", "reactome_id": "R-ALL-159160", "label": "BMG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16427", "formula": "C39H44N4O12", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-158602", "reactome_id": "R-ALL-158602", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "glucuronosyltransferase activity of UGT1A1 [endoplasmic reticulum membrane]", "activity_id": "9632040", "ec_numbers": [ "2.4.1.17" ], "accessions": [ "P22309" ] } ], "citations": [ { "id": "PMID:8027054", "title": "Bilirubin UDP-glucuronosyltransferase 1 is the only relevant bilirubin glucuronidating isoform in man", "journal": "J Biol Chem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8027054/" }, { "id": "PMID:1339448", "title": "A novel complex locus UGT1 encodes human bilirubin, phenol, and other UDP-glucuronosyltransferase isozymes with identical carboxyl termini", "journal": "J. Biol. Chem.", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1339448/" } ], "x": 7078.666666666667, "y": 3011.3928571428573, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9638046", "label": "ACY3:Zn2+ dimer hydrolyses mercapturic acids", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:36856" ], "source_url": "https://reactome.org/content/detail/R-HSA-9638046", "summary": "ACY3:Zn2+ dimer hydrolyses mercapturic acids is a Reactome release 97 curated reaction event. The source record connects AFXBO-NAC,AFNBO-NAC, H2O to AFXBO-C,AFNBO-C, acetate in cytosol and cross-references RHEA:36856.", "equation": "AFXBO-NAC,AFNBO-NAC + H2O → AFXBO-C,AFNBO-C + acetate", "inputs": [ { "node_id": "R-ALL-5490236", "reactome_id": "R-ALL-5490236", "label": "AFXBO-NAC,AFNBO-NAC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5490231", "reactome_id": "R-ALL-5490231", "label": "AFXBO-C,AFNBO-C", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-9717939", "reactome_id": "R-ALL-9717939", "label": "acetate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aminoacylase activity of ACY3:Zn2+ dimer [cytosol]", "activity_id": "9638047", "ec_numbers": [ "3.5.1.14" ], "accessions": [] } ], "citations": [ { "id": "PMID:12933810", "title": "Essential roles of zinc ligation and enzyme dimerization for catalysis in the aminoacylase-1/M20 family", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12933810/" } ], "x": 7740, "y": 2936.4803921568628, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9638075", "label": "HEXB cleaves the terminal GalNAc from DS", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9638075", "summary": "HEXB cleaves the terminal GalNAc from DS is a Reactome release 97 curated reaction event. The source record connects H2O, ChEBI:63516 chain to GalNAc, IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl in lysosomal lumen.", "equation": "H2O + ChEBI:63516 chain → GalNAc + IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2065269", "reactome_id": "R-ALL-2065269", "label": "ChEBI:63516 chain", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:747395", "formula": "C45H69N2O40S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605658", "reactome_id": "R-ALL-1605658", "label": "GalNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28037", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2105025", "reactome_id": "R-ALL-2105025", "label": "IdoA-GalNAc(4S)-GlcA-Gal-Gal-Xyl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63873", "formula": "C37H59NO35S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXB [lysosomal lumen]", "activity_id": "9638074", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:2971395", "title": "Oligosaccharide structure and amino acid sequence of the major glycopeptides of mature human beta-hexosaminidase", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2971395/" } ], "x": 2381.3333333333335, "y": 983.6515151515151, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9638076", "label": "HEXB cleaves the terminal GlcNAc from small HA fragments", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9638076", "summary": "HEXB cleaves the terminal GlcNAc from small HA fragments is a Reactome release 97 curated reaction event. The source record connects H2O, GlcNAc-GlcA-GlcNAc to GlcNAc, GlcA-β1,3-GlcNAc in lysosomal lumen.", "equation": "H2O + GlcNAc-GlcA-GlcNAc → GlcNAc + GlcA-β1,3-GlcNAc", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2162223", "reactome_id": "R-ALL-2162223", "label": "GlcNAc-GlcA-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "OtherEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1678743", "reactome_id": "R-ALL-1678743", "label": "GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17411", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-2162224", "reactome_id": "R-ALL-2162224", "label": "GlcA-β1,3-GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64024", "formula": "C14H23NO12", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXB [lysosomal lumen]", "activity_id": "9638077", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:2971395", "title": "Oligosaccharide structure and amino acid sequence of the major glycopeptides of mature human beta-hexosaminidase", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2971395/" } ], "x": 2381.3333333333335, "y": 1019.0151515151515, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9638078", "label": "HEXB cleaves the terminal GlcNAc from keratan sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9638078", "summary": "HEXB cleaves the terminal GlcNAc from keratan sulfate is a Reactome release 97 curated reaction event. The source record connects H2O, GlcNAc-Gal-GlcNAc(S)-Gal to Gal-GlcNAc(S)-Gal, GlcNAc in lysosomal lumen.", "equation": "H2O + GlcNAc-Gal-GlcNAc(S)-Gal → Gal-GlcNAc(S)-Gal + GlcNAc", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2105012", "reactome_id": "R-ALL-2105012", "label": "GlcNAc-Gal-GlcNAc(S)-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:63852", "formula": "C28H48N2O24S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2105010", "reactome_id": "R-ALL-2105010", "label": "Gal-GlcNAc(S)-Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63853", "formula": "C20H35NO19S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1678743", "reactome_id": "R-ALL-1678743", "label": "GlcNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17411", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXB [lysosomal lumen]", "activity_id": "9638079", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:2971395", "title": "Oligosaccharide structure and amino acid sequence of the major glycopeptides of mature human beta-hexosaminidase", "journal": "Biochemistry", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/2971395/" } ], "x": 2660, "y": 983.6515151515151, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9638125", "label": "PGM1:Mg2+ isomerises G1P to G6P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "galactose", "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23537" ], "source_url": "https://reactome.org/content/detail/R-HSA-9638125", "summary": "PGM1:Mg2+ isomerises G1P to G6P is a Reactome release 97 curated reaction event. The source record connects G1P to G6P in cytosol and cross-references RHEA:23537.", "equation": "G1P → G6P", "inputs": [ { "node_id": "R-ALL-29548", "reactome_id": "R-ALL-29548", "label": "G1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58601", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphoglucomutase activity of PGM1:Mg2+ [cytosol]", "activity_id": "9638129", "ec_numbers": [ "5.4.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:7902568", "title": "The classical human phosphoglucomutase (PGM1) isozyme polymorphism is generated by intragenic recombination", "journal": "Proc Natl Acad Sci U S A", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7902568/" }, { "id": "PMID:4829444", "title": "A kinetic study of the isozymes determined by the three human phosphoglucomutase loci PGM1, PGM2, and PGM3", "journal": "Eur J Biochem", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4829444/" } ], "x": 1808.6666666666667, "y": 756.9615384615385, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9638127", "label": "PGM1:Mg2+ isomerises G6P to G1P", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:23538" ], "source_url": "https://reactome.org/content/detail/R-HSA-9638127", "summary": "PGM1:Mg2+ isomerises G6P to G1P is a Reactome release 97 curated reaction event. The source record connects G6P to G1P in cytosol and cross-references RHEA:23538.", "equation": "G6P → G1P", "inputs": [ { "node_id": "R-ALL-30537", "reactome_id": "R-ALL-30537", "label": "G6P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58225", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29548", "reactome_id": "R-ALL-29548", "label": "G1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58601", "formula": "C6H11O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphoglucomutase activity of PGM1:Mg2+ [cytosol]", "activity_id": "9638129", "ec_numbers": [ "5.4.2.2" ], "accessions": [] } ], "citations": [ { "id": "PMID:7902568", "title": "The classical human phosphoglucomutase (PGM1) isozyme polymorphism is generated by intragenic recombination", "journal": "Proc Natl Acad Sci U S A", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7902568/" }, { "id": "PMID:4829444", "title": "A kinetic study of the isozymes determined by the three human phosphoglucomutase loci PGM1, PGM2, and PGM3", "journal": "Eur J Biochem", "year": 1974, "url": "https://pubmed.ncbi.nlm.nih.gov/4829444/" } ], "x": 1251.3333333333333, "y": 786.8846153846154, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9640302", "label": "Asc.- radical dissociates to AscH- and DeHA", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:30268" ], "source_url": "https://reactome.org/content/detail/R-HSA-9640302", "summary": "Asc.- radical dissociates to AscH- and DeHA is a Reactome release 97 curated reaction event. The source record connects Asc.-, H+ to AscH-, DeHA in cytosol and cross-references RHEA:30268.", "equation": "2 Asc.- + H+ → AscH- + DeHA", "inputs": [ { "node_id": "R-ALL-1497805", "reactome_id": "R-ALL-1497805", "label": "Asc.-", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:59513", "formula": "C6H6O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-198816", "reactome_id": "R-ALL-198816", "label": "AscH-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:38290", "formula": "C6H7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-198827", "reactome_id": "R-ALL-198827", "label": "DeHA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58539", "formula": "C6H5O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:22728050", "title": "Ascorbic acid: chemistry, biology and the treatment of cancer", "journal": "Biochim. Biophys. Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22728050/" } ], "x": 5391.333333333333, "y": 3033.328947368421, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9640307", "label": "DeHA hydrolyses to 2,3-DKG", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:70300" ], "source_url": "https://reactome.org/content/detail/R-HSA-9640307", "summary": "DeHA hydrolyses to 2,3-DKG is a Reactome release 97 curated reaction event. The source record connects DeHA, H2O to 2,3-DKG in cytosol and cross-references RHEA:70300.", "equation": "DeHA + H2O → 2,3-DKG", "inputs": [ { "node_id": "R-ALL-198827", "reactome_id": "R-ALL-198827", "label": "DeHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58539", "formula": "C6H5O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9640308", "reactome_id": "R-ALL-9640308", "label": "2,3-DKG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57441", "formula": "C6H7O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10727845", "title": "The non-oxidative degradation of ascorbic acid at physiological conditions", "journal": "Biochim. Biophys. Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10727845/" } ], "x": 5670, "y": 3033.328947368421, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9640316", "label": "DeHA hydrolyses to threonate and oxalate", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9640316", "summary": "DeHA hydrolyses to threonate and oxalate is a Reactome release 97 curated reaction event. The source record connects DeHA, H2O, H2O2 to oxalate, threonate in cytosol.", "equation": "DeHA + H2O + H2O2 → oxalate + threonate", "inputs": [ { "node_id": "R-ALL-198827", "reactome_id": "R-ALL-198827", "label": "DeHA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58539", "formula": "C6H5O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9640314", "reactome_id": "R-ALL-9640314", "label": "oxalate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30623", "formula": "C2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9640313", "reactome_id": "R-ALL-9640313", "label": "threonate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57561", "formula": "C4H7O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10727845", "title": "The non-oxidative degradation of ascorbic acid at physiological conditions", "journal": "Biochim. Biophys. Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10727845/" } ], "x": 5391.333333333333, "y": 3094.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9640321", "label": "2,3-DKG hydrolyses to ERU and oxalate", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9640321", "summary": "2,3-DKG hydrolyses to ERU and oxalate is a Reactome release 97 curated reaction event. The source record connects 2,3-DKG, H2O to ERU, oxalate, H+ in cytosol.", "equation": "2,3-DKG + H2O → ERU + oxalate + H+", "inputs": [ { "node_id": "R-ALL-9640308", "reactome_id": "R-ALL-9640308", "label": "2,3-DKG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57441", "formula": "C6H7O7", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9640306", "reactome_id": "R-ALL-9640306", "label": "ERU", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:27913", "formula": "C4H8O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9640314", "reactome_id": "R-ALL-9640314", "label": "oxalate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30623", "formula": "C2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [], "citations": [ { "id": "PMID:10727845", "title": "The non-oxidative degradation of ascorbic acid at physiological conditions", "journal": "Biochim. Biophys. Acta", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10727845/" } ], "x": 5670, "y": 3094.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-964958", "label": "2xPDXK:2xZn2+ phosphorylates PXA", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25105" ], "source_url": "https://reactome.org/content/detail/R-HSA-964958", "summary": "2xPDXK:2xZn2+ phosphorylates PXA is a Reactome release 97 curated reaction event. The source record connects PXA, ATP to PXAP, ADP in cytosol and cross-references RHEA:25105.", "equation": "PXA + ATP → PXAP + ADP", "inputs": [ { "node_id": "R-ALL-964945", "reactome_id": "R-ALL-964945", "label": "PXA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16410", "formula": "C8H12N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-965146", "reactome_id": "R-ALL-965146", "label": "PXAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18335", "formula": "C8H13N2O5P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyridoxal kinase activity of 2xPDXK:2xZn2+ [cytosol]", "activity_id": "965111", "ec_numbers": [ "2.7.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:15249053", "title": "Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases", "journal": "Protein Expr Purif", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15249053/" }, { "id": "PMID:10987144", "title": "Human pyridoxal kinase: overexpression and properties of the recombinant enzyme", "journal": "Mol Cells", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10987144/" } ], "x": 5391.333333333333, "y": 2982.1447368421054, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-964962", "label": "2xPDKX:2xZn2+ phosphorylates PDX", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:25109" ], "source_url": "https://reactome.org/content/detail/R-HSA-964962", "summary": "2xPDKX:2xZn2+ phosphorylates PDX is a Reactome release 97 curated reaction event. The source record connects PDX, ATP to PDXP, ADP in cytosol and cross-references RHEA:25109.", "equation": "PDX + ATP → PDXP + ADP", "inputs": [ { "node_id": "R-ALL-965053", "reactome_id": "R-ALL-965053", "label": "PDX", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16709", "formula": "C8H11NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-964952", "reactome_id": "R-ALL-964952", "label": "PDXP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28803", "formula": "C8H12NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyridoxal kinase activity of 2xPDXK:2xZn2+ [cytosol]", "activity_id": "965111", "ec_numbers": [ "2.7.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:15249053", "title": "Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases", "journal": "Protein Expr Purif", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15249053/" }, { "id": "PMID:10987144", "title": "Human pyridoxal kinase: overexpression and properties of the recombinant enzyme", "journal": "Mol Cells", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10987144/" } ], "x": 5670, "y": 2982.1447368421054, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-964970", "label": "2xPDXK:2xZn2+ phosphorylates PXL", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:10225" ], "source_url": "https://reactome.org/content/detail/R-HSA-964970", "summary": "2xPDXK:2xZn2+ phosphorylates PXL is a Reactome release 97 curated reaction event. The source record connects PXL, ATP to PXLP, ADP in cytosol and cross-references RHEA:10225.", "equation": "PXL + ATP → PXLP + ADP", "inputs": [ { "node_id": "R-ALL-965131", "reactome_id": "R-ALL-965131", "label": "PXL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17310", "formula": "C8H9NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29390", "reactome_id": "R-ALL-29390", "label": "PXLP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:597326", "formula": "C8H8NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "pyridoxal kinase activity of 2xPDXK:2xZn2+ [cytosol]", "activity_id": "965111", "ec_numbers": [ "2.7.1.35" ], "accessions": [] } ], "citations": [ { "id": "PMID:15249053", "title": "Expression, purification, and kinetic constants for human and Escherichia coli pyridoxal kinases", "journal": "Protein Expr Purif", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15249053/" }, { "id": "PMID:10987144", "title": "Human pyridoxal kinase: overexpression and properties of the recombinant enzyme", "journal": "Mol Cells", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10987144/" } ], "x": 5948.666666666667, "y": 2982.1447368421054, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-965019", "label": "2xPNPO:2xFMN oxidizes PDXP to PXLP", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15150" ], "source_url": "https://reactome.org/content/detail/R-HSA-965019", "summary": "2xPNPO:2xFMN oxidizes PDXP to PXLP is a Reactome release 97 curated reaction event. The source record connects PDXP, O2 to PXLP, H2O2 in cytosol and cross-references RHEA:15150.", "equation": "PDXP + O2 → PXLP + H2O2", "inputs": [ { "node_id": "R-ALL-964952", "reactome_id": "R-ALL-964952", "label": "PDXP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28803", "formula": "C8H12NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29390", "reactome_id": "R-ALL-29390", "label": "PXLP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:597326", "formula": "C8H8NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pyridoxamine phosphate oxidase activity of 2xPNPO:2xFMN [cytosol]", "activity_id": "965270", "ec_numbers": [ "1.4.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:15182361", "title": "Genomic organization, tissue distribution and deletion mutation of human pyridoxine 5'-phosphate oxidase", "journal": "Eur J Biochem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15182361/" } ], "x": 5391.333333333333, "y": 2992.3815789473683, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-965067", "label": "THTPA:Mg2+ hydrolyzes ThTP to TDP", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11745" ], "source_url": "https://reactome.org/content/detail/R-HSA-965067", "summary": "THTPA:Mg2+ hydrolyzes ThTP to TDP is a Reactome release 97 curated reaction event. The source record connects ThTP, H2O to ThDP, Pi in cytosol and cross-references RHEA:11745.", "equation": "ThTP + H2O → ThDP + Pi", "inputs": [ { "node_id": "R-ALL-964965", "reactome_id": "R-ALL-964965", "label": "ThTP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:9534", "formula": "C12H20N4O10P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29480", "reactome_id": "R-ALL-29480", "label": "ThDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58937", "formula": "C12H16N4O7P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "thiamine triphosphate phosphatase activity of THTPA:Mg2+ [cytosol]", "activity_id": "964942", "ec_numbers": [ "3.6.1.28" ], "accessions": [] } ], "citations": [ { "id": "PMID:11827967", "title": "Molecular characterization of a specific thiamine triphosphatase widely expressed in mammalian tissues", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11827967/" } ], "x": 5670, "y": 2992.3815789473683, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-965079", "label": "2xPNPO:2xFMN oxidizes PXAP to PXLP", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15818" ], "source_url": "https://reactome.org/content/detail/R-HSA-965079", "summary": "2xPNPO:2xFMN oxidizes PXAP to PXLP is a Reactome release 97 curated reaction event. The source record connects PXAP, H2O, O2 to PXLP, NH4+, H2O2 in cytosol and cross-references RHEA:15818.", "equation": "PXAP + H2O + O2 → PXLP + NH4+ + H2O2", "inputs": [ { "node_id": "R-ALL-965146", "reactome_id": "R-ALL-965146", "label": "PXAP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18335", "formula": "C8H13N2O5P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29390", "reactome_id": "R-ALL-29390", "label": "PXLP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:597326", "formula": "C8H8NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pyridoxamine phosphate oxidase activity of 2xPNPO:2xFMN [cytosol]", "activity_id": "965270", "ec_numbers": [ "1.4.3.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:15182361", "title": "Genomic organization, tissue distribution and deletion mutation of human pyridoxine 5'-phosphate oxidase", "journal": "Eur J Biochem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15182361/" } ], "x": 5670, "y": 2900.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9660192", "label": "VDR agonists bind VDR", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9660192", "summary": "VDR agonists bind VDR is a Reactome release 97 curated reaction event. The source record connects VDR, VDR agonists to VDR:VDR agonists in nucleoplasm.", "equation": "VDR + VDR agonists → VDR:VDR agonists", "inputs": [ { "node_id": "R-HSA-375512", "reactome_id": "R-HSA-375512", "label": "VDR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9660116", "reactome_id": "R-ALL-9660116", "label": "VDR agonists", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9660135", "reactome_id": "R-HSA-9660135", "label": "VDR:VDR agonists", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24569127", "title": "Active vitamin D analogs, maxacalcitol and alfacalcidol, as maintenance therapy for mild secondary hyperparathyroidism in hemodialysis patients - a randomized study", "journal": "Int J Clin Pharmacol Ther", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24569127/" }, { "id": "PMID:28841848", "title": "Comparative efficacy and safety of paricalcitol versus vitamin D receptor activators for dialysis patients with secondary hyperparathyroidism: a meta-analysis of randomized controlled trials", "journal": "BMC Nephrol", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28841848/" }, { "id": "PMID:26748501", "title": "Comparison between paricalcitol and active non-selective vitamin D receptor activator for secondary hyperparathyroidism in chronic kidney disease: a systematic review and meta-analysis of randomized controlled trials", "journal": "Int Urol Nephrol", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26748501/" }, { "id": "PMID:19473635", "title": "Comparison of oral falecalcitriol and intravenous calcitriol in hemodialysis patients with secondary hyperparathyroidism: a randomized, crossover trial", "journal": "Clin. Nephrol.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19473635/" }, { "id": "PMID:25363733", "title": "Comparison of paricalcitol with maxacalcitol injection in Japanese hemodialysis patients with secondary hyperparathyroidism", "journal": "Ther Apher Dial", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25363733/" }, { "id": "PMID:11234559", "title": "Double-blind, placebo-controlled, randomized, right-left study comparing calcipotriol monotherapy with a combined treatment of calcipotriol and diflucortolone valerate in chronic plaque psoriasis", "journal": "Acta Derm Venereol Suppl (Stockh)", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11234559/" } ], "x": 4451.333333333333, "y": 1830.6439393939395, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9661397", "label": "SLCO1B1 transports BIL from extracellular region (blood) to cytosol (hepatocyte)", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · extracellular region · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661397", "summary": "SLCO1B1 transports BIL from extracellular region (blood) to cytosol (hepatocyte) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects BIL to BIL in cytosol · extracellular region · plasma membrane.", "equation": "BIL → BIL", "inputs": [ { "node_id": "R-ALL-9661418", "reactome_id": "R-ALL-9661418", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189386", "reactome_id": "R-ALL-189386", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "secondary active transmembrane transporter activity of SLCO1B1 [plasma membrane]", "activity_id": "9661435", "ec_numbers": null, "accessions": [ "Q9Y6L6" ] } ], "citations": [ { "id": "PMID:10644574", "title": "A novel human organic anion transporting polypeptide localized to the basolateral hepatocyte membrane", "journal": "Am J Physiol Gastrointest Liver Physiol", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10644574/" }, { "id": "PMID:11134001", "title": "Hepatic uptake of bilirubin and its conjugates by the human organic anion transporter SLC21A6", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11134001/" } ], "x": 7078.666666666667, "y": 3039.1785714285716, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9661405", "label": "ABCC1 transports BIL from cytosol to extracellular region (blood)", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661405", "summary": "ABCC1 transports BIL from cytosol to extracellular region (blood) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects BIL to BIL in cytosol · extracellular region.", "equation": "BIL → BIL", "inputs": [ { "node_id": "R-ALL-189386", "reactome_id": "R-ALL-189386", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9661418", "reactome_id": "R-ALL-9661418", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ABC-type transporter activity of ABCC1 [plasma membrane]", "activity_id": "266058", "ec_numbers": null, "accessions": [ "P33527" ] } ], "citations": [ { "id": "PMID:11023036", "title": "Recent advances in bilirubin metabolism research: the molecular mechanism of hepatocyte bilirubin transport and its clinical relevance", "journal": "J. Gastroenterol.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11023036/" }, { "id": "PMID:15245331", "title": "The human multidrug-resistance-associated protein MRP1 mediates ATP-dependent transport of unconjugated bilirubin", "journal": "Biochem. J.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15245331/" } ], "x": 6521.333333333333, "y": 3066.964285714286, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9661408", "label": "FLVCR1-2 transports heme from mitochondrial matrix to cytosol", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · mitochondrial matrix · mitochondrial inner membrane", "compartment_ids": [ "GO:0005829", "GO:0005759", "GO:0005743" ], "rhea_ids": [ "RHEA:75443" ], "source_url": "https://reactome.org/content/detail/R-HSA-9661408", "summary": "FLVCR1-2 transports heme from mitochondrial matrix to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects heme to ferroheme in cytosol · mitochondrial matrix · mitochondrial inner membrane and cross-references RHEA:75443.", "equation": "heme → ferroheme", "inputs": [ { "node_id": "R-ALL-189444", "reactome_id": "R-ALL-189444", "label": "heme", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60344", "formula": "C34H30FeN4O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-71185", "reactome_id": "R-ALL-71185", "label": "ferroheme", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60344", "formula": "C34H30FeN4O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "heme transmembrane transporter activity of FLVCR1-2 [mitochondrial inner membrane]", "activity_id": "9661443", "ec_numbers": null, "accessions": [ "Q9Y5Y0" ] } ], "citations": [ { "id": "PMID:23187127", "title": "The mitochondrial heme exporter FLVCR1b mediates erythroid differentiation", "journal": "J. Clin. Invest.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23187127/" } ], "x": 6800, "y": 2928.035714285714, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9661417", "label": "ABCG2 tetramer transports BMG,BDG from cytosol to extracellular region", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · extracellular region · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661417", "summary": "ABCG2 tetramer transports BMG,BDG from cytosol to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects BMG,BDG, ATP, H2O to BMG,BDG, ADP, Pi in cytosol · extracellular region · plasma membrane.", "equation": "BMG,BDG + ATP + H2O → BMG,BDG + ADP + Pi", "inputs": [ { "node_id": "R-ALL-5679042", "reactome_id": "R-ALL-5679042", "label": "BMG,BDG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-5679034", "reactome_id": "R-ALL-5679034", "label": "BMG,BDG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "bilirubin transmembrane transporter activity of ABCG2 tetramer [plasma membrane]", "activity_id": "9661400", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15001581", "title": "Characterization of oligomeric human half-ABC transporter ATP-binding cassette G2", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15001581/" } ], "x": 6521.333333333333, "y": 3039.1785714285716, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9661419", "label": "ALB binds extracellular heme", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661419", "summary": "ALB binds extracellular heme is a Reactome release 97 curated reaction event. The source record connects ALB, heme to ALB:heme in extracellular region.", "equation": "ALB + heme → ALB:heme", "inputs": [ { "node_id": "R-HSA-194115", "reactome_id": "R-HSA-194115", "label": "ALB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-917877", "reactome_id": "R-ALL-917877", "label": "heme", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60344", "formula": "C34H30FeN4O4", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9661451", "reactome_id": "R-HSA-9661451", "label": "ALB:heme", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "heme binding activity of ALB [extracellular region]", "activity_id": "9955845", "ec_numbers": null, "accessions": [ "P02768" ] } ], "citations": [ { "id": "PMID:36979511", "title": "Heme Scavenging and Delivery: The Role of Human Serum Albumin", "journal": "Biomolecules", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36979511/" }, { "id": "PMID:20705604", "title": "ABCG2 transports and transfers heme to albumin through its large extracellular loop", "journal": "J. Biol. Chem.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20705604/" } ], "x": 6521.333333333333, "y": 3094.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9661425", "label": "BIL:ALB dissociates", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661425", "summary": "BIL:ALB dissociates is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects BIL:ALB to BIL, ALB in extracellular region.", "equation": "BIL:ALB → BIL + ALB", "inputs": [ { "node_id": "R-HSA-9661438", "reactome_id": "R-HSA-9661438", "label": "BIL:ALB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9661418", "reactome_id": "R-ALL-9661418", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-194115", "reactome_id": "R-HSA-194115", "label": "ALB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:11134001", "title": "Hepatic uptake of bilirubin and its conjugates by the human organic anion transporter SLC21A6", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11134001/" } ], "x": 6800, "y": 3178.1071428571427, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9661432", "label": "BIL binds ALB", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661432", "summary": "BIL binds ALB is a Reactome release 97 curated reaction event. The source record connects BIL, ALB to BIL:ALB in extracellular region.", "equation": "BIL + ALB → BIL:ALB", "inputs": [ { "node_id": "R-ALL-9661418", "reactome_id": "R-ALL-9661418", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-194115", "reactome_id": "R-HSA-194115", "label": "ALB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9661438", "reactome_id": "R-HSA-9661438", "label": "BIL:ALB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of ALB [extracellular region]", "activity_id": "9955838", "ec_numbers": null, "accessions": [ "P02768" ] } ], "citations": [ { "id": "PMID:1157293", "title": "The albumin binding of unconjugated bilirubin in serum", "journal": "Clin. Biochem.", "year": 1975, "url": "https://pubmed.ncbi.nlm.nih.gov/1157293/" }, { "id": "PMID:11397817", "title": "Affinity of human serum albumin for bilirubin varies with albumin concentration and buffer composition: results of a novel ultrafiltration method", "journal": "J. Biol. Chem.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11397817/" } ], "x": 6800, "y": 3094.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9661446", "label": "BMG, BDG translocates from ER lumen to cytosol", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · endoplasmic reticulum lumen · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005788", "GO:0005789" ], "rhea_ids": [ "RHEA:72795", "RHEA:72791" ], "source_url": "https://reactome.org/content/detail/R-HSA-9661446", "summary": "BMG, BDG translocates from ER lumen to cytosol is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects BMG,BDG to BMG,BDG in cytosol · endoplasmic reticulum lumen · endoplasmic reticulum membrane and cross-references RHEA:72795, RHEA:72791.", "equation": "BMG,BDG → BMG,BDG", "inputs": [ { "node_id": "R-ALL-9661424", "reactome_id": "R-ALL-9661424", "label": "BMG,BDG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5679042", "reactome_id": "R-ALL-5679042", "label": "BMG,BDG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "secondary active transmembrane transporter activity of BGET [endoplasmic reticulum membrane]", "activity_id": "9663472", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:23500526", "title": "The UDP-glucuronosyltransferases: their role in drug metabolism and detoxification", "journal": "Int. J. Biochem. Cell Biol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23500526/" }, { "id": "PMID:24704527", "title": "Inherited disorders of bilirubin transport and conjugation: new insights into molecular mechanisms and consequences", "journal": "Gastroenterology", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24704527/" } ], "x": 6521.333333333333, "y": 2816.8928571428573, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9661710", "label": "An unknown oxidase oxidises D-UBGN to UBN", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661710", "summary": "An unknown oxidase oxidises D-UBGN to UBN is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects D-UBGN, an oxidized electron acceptor to UBN, a reduced electron acceptor in extracellular region.", "equation": "D-UBGN + an oxidized electron acceptor → UBN + a reduced electron acceptor", "inputs": [ { "node_id": "R-ALL-9661518", "reactome_id": "R-ALL-9661518", "label": "D-UBGN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:4260", "formula": "C33H42N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9661729", "reactome_id": "R-ALL-9661729", "label": "an oxidized electron acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17654", "formula": "", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9661716", "reactome_id": "R-ALL-9661716", "label": "UBN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36378", "formula": "C33H42N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9661699", "reactome_id": "R-ALL-9661699", "label": "a reduced electron acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17654", "formula": "", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-CH group of donors of UBGNO [extracellular region]", "activity_id": "9661731", "ec_numbers": [ "1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:6456089", "title": "Urinary urobilinogen determined by a mercuric chloride procedure", "journal": "Clin. Chem.", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/6456089/" } ], "x": 6800, "y": 3122.535714285714, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9661723", "label": "SLCO2B1-3 transports BIL from extracellular region (blood) to cytosol (hepatocyte)", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "plasma membrane · cytosol · extracellular region", "compartment_ids": [ "GO:0005886", "GO:0005829", "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661723", "summary": "SLCO2B1-3 transports BIL from extracellular region (blood) to cytosol (hepatocyte) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects BIL to BIL in plasma membrane · cytosol · extracellular region.", "equation": "BIL → BIL", "inputs": [ { "node_id": "R-ALL-9661418", "reactome_id": "R-ALL-9661418", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189386", "reactome_id": "R-ALL-189386", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "secondary active transmembrane transporter activity of SLCO2B1-3 [plasma membrane]", "activity_id": "9661702", "ec_numbers": null, "accessions": [ "O94956" ] } ], "citations": [ { "id": "PMID:11159893", "title": "Organic anion-transporting polypeptide B (OATP-B) and its functional comparison with three other OATPs of human liver", "journal": "Gastroenterology", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11159893/" }, { "id": "PMID:23531488", "title": "Transport function and transcriptional regulation of a liver-enriched human organic anion transporting polypeptide 2B1 transcriptional start site variant", "journal": "Mol. Pharmacol.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23531488/" } ], "x": 6800, "y": 3066.964285714286, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9661726", "label": "An unknown reductase reduces D-UBGN to STBN", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661726", "summary": "An unknown reductase reduces D-UBGN to STBN is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects D-UBGN, a reduced electron acceptor to STBN, an oxidized electron acceptor in extracellular region.", "equation": "D-UBGN + a reduced electron acceptor → STBN + an oxidized electron acceptor", "inputs": [ { "node_id": "R-ALL-9661518", "reactome_id": "R-ALL-9661518", "label": "D-UBGN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:4260", "formula": "C33H42N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9661699", "reactome_id": "R-ALL-9661699", "label": "a reduced electron acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17654", "formula": "", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9661704", "reactome_id": "R-ALL-9661704", "label": "STBN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:26756", "formula": "C33H46N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9661729", "reactome_id": "R-ALL-9661729", "label": "an oxidized electron acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17654", "formula": "", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor of UBGNR [extracellular region]", "activity_id": "9661700", "ec_numbers": [ "1.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:16504607", "title": "Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora", "journal": "J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16504607/" } ], "x": 7078.666666666667, "y": 3122.535714285714, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9661745", "label": "An unknown BILR reduces BIL to D-UBGN", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661745", "summary": "An unknown BILR reduces BIL to D-UBGN is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects BIL, a reduced electron acceptor to D-UBGN, an oxidized electron acceptor in extracellular region.", "equation": "BIL + a reduced electron acceptor → D-UBGN + an oxidized electron acceptor", "inputs": [ { "node_id": "R-ALL-9661418", "reactome_id": "R-ALL-9661418", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9661699", "reactome_id": "R-ALL-9661699", "label": "a reduced electron acceptor", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17654", "formula": "", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9661518", "reactome_id": "R-ALL-9661518", "label": "D-UBGN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:4260", "formula": "C33H42N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9661729", "reactome_id": "R-ALL-9661729", "label": "an oxidized electron acceptor", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17654", "formula": "", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor of BILR [extracellular region]", "activity_id": "9661735", "ec_numbers": [ "1.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:5652509", "title": "Synthesis of urobilinogen by broken cell preparations of intestinal bacteria", "journal": "Gastroenterology", "year": 1968, "url": "https://pubmed.ncbi.nlm.nih.gov/5652509/" }, { "id": "PMID:13591316", "title": "Preferential reduction of conjugated bilirubin to urobilinogen by normal fecal flora", "journal": "Proc. Soc. Exp. Biol. Med.", "year": 1958, "url": "https://pubmed.ncbi.nlm.nih.gov/13591316/" }, { "id": "PMID:16504607", "title": "Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora", "journal": "J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16504607/" }, { "id": "PMID:22540115", "title": "Reduction of bilirubin ditaurate by the intestinal bacterium Clostridium perfringens", "journal": "Acta Biochim. Pol.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22540115/" } ], "x": 6521.333333333333, "y": 3150.3214285714284, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9661799", "label": "SLCO1B3 transports BIL from extracellular region (blood) to cytosol (hepatocyte)", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol · extracellular region · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661799", "summary": "SLCO1B3 transports BIL from extracellular region (blood) to cytosol (hepatocyte) is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects BIL to BIL in cytosol · extracellular region · plasma membrane.", "equation": "BIL → BIL", "inputs": [ { "node_id": "R-ALL-9661418", "reactome_id": "R-ALL-9661418", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189386", "reactome_id": "R-ALL-189386", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "secondary active transmembrane transporter activity of SLCO1B3 [plasma membrane]", "activity_id": "9661798", "ec_numbers": null, "accessions": [ "Q9NPD5" ] } ], "citations": [ { "id": "PMID:22232210", "title": "Complete OATP1B1 and OATP1B3 deficiency causes human Rotor syndrome by interrupting conjugated bilirubin reuptake into the liver", "journal": "J. Clin. Invest.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22232210/" } ], "x": 7078.666666666667, "y": 3066.964285714286, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9661820", "label": "Bacterial GUSB hydrolyses BDG to BIL", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9661820", "summary": "Bacterial GUSB hydrolyses BDG to BIL is a Reactome release 97 curated reaction event. The source record connects BDG, NADPH, H2O to NADP+, GlcA, BIL in extracellular region.", "equation": "BDG + 3 NADPH + 2 H2O → 3 NADP+ + 2 GlcA + BIL", "inputs": [ { "node_id": "R-ALL-5679019", "reactome_id": "R-ALL-5679019", "label": "BDG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58471", "formula": "C45H50N4O18", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-351627", "reactome_id": "R-ALL-351627", "label": "NADPH", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-109276", "reactome_id": "R-ALL-109276", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-351628", "reactome_id": "R-ALL-351628", "label": "NADP+", "role": "output", "stoichiometry": 3, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9661481", "reactome_id": "R-ALL-9661481", "label": "GlcA", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15748", "formula": "C6H9O7", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9661418", "reactome_id": "R-ALL-9661418", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity, hydrolyzing O-glycosyl compounds of GUSB [extracellular region]", "activity_id": "9661510", "ec_numbers": [ "3.2.1" ], "accessions": [ "Q8VNV4" ] } ], "citations": [ { "id": "PMID:8845801", "title": "Purification and characterization of beta-glucuronidase from Escherichia coli HGU-3, a human intestinal bacterium", "journal": "Biol. Pharm. Bull.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8845801/" }, { "id": "PMID:11522976", "title": "Expression of bacterial beta-glucuronidase in human bile: an in vitro study", "journal": "Gastrointest. Endosc.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11522976/" }, { "id": "PMID:793184", "title": "[Bilirubin metabolism (author's transl)]", "journal": "Wien. Klin. Wochenschr.", "year": 1976, "url": "https://pubmed.ncbi.nlm.nih.gov/793184/" } ], "x": 6800, "y": 2955.8214285714284, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9663492", "label": "BIL dissociates from GSTA1, FABP1", "schema_class": "BlackBoxEvent", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9663492", "summary": "BIL dissociates from GSTA1, FABP1 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects BIL:GSTA1, FABP1 to BIL, GSTA1, FABP1 in cytosol.", "equation": "BIL:GSTA1, FABP1 → BIL + GSTA1, FABP1", "inputs": [ { "node_id": "R-HSA-9663490", "reactome_id": "R-HSA-9663490", "label": "BIL:GSTA1, FABP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-189386", "reactome_id": "R-ALL-189386", "label": "BIL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9663507", "reactome_id": "R-HSA-9663507", "label": "GSTA1, FABP1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:7372607", "title": "Bilirubin binding to human liver ligandin (glutathione S-transferase)", "journal": "J. Biol. Chem.", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/7372607/" }, { "id": "PMID:4980931", "title": "Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions", "journal": "J. Clin. Invest.", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/4980931/" } ], "x": 7078.666666666667, "y": 3178.1071428571427, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9663511", "label": "BIL binds GSTA1, FABP1", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "porphyrins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9663511", "summary": "BIL binds GSTA1, FABP1 is a Reactome release 97 curated reaction event. The source record connects BIL, GSTA1, FABP1 to BIL:GSTA1, FABP1 in cytosol.", "equation": "BIL + GSTA1, FABP1 → BIL:GSTA1, FABP1", "inputs": [ { "node_id": "R-ALL-189386", "reactome_id": "R-ALL-189386", "label": "BIL", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57977", "formula": "C33H34N4O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9663507", "reactome_id": "R-HSA-9663507", "label": "GSTA1, FABP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9663490", "reactome_id": "R-HSA-9663490", "label": "BIL:GSTA1, FABP1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "molecular carrier activity of GSTA1, FABP1 [cytosol]", "activity_id": "9955861", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:7372607", "title": "Bilirubin binding to human liver ligandin (glutathione S-transferase)", "journal": "J. Biol. Chem.", "year": 1980, "url": "https://pubmed.ncbi.nlm.nih.gov/7372607/" }, { "id": "PMID:4980931", "title": "Two hepatic cytoplasmic protein fractions, Y and Z, and their possible role in the hepatic uptake of bilirubin, sulfobromophthalein, and other anions", "journal": "J. Clin. Invest.", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/4980931/" } ], "x": 7078.666666666667, "y": 3094.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9672770", "label": "SLC25A44 transports Leu, Ile and Val from cytosol to mitochondrial matrix", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [ "RHEA:70943", "RHEA:73011", "RHEA:29703" ], "source_url": "https://reactome.org/content/detail/R-HSA-9672770", "summary": "SLC25A44 transports Leu, Ile and Val from cytosol to mitochondrial matrix is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects Leu, Ile, Val to Leu, Ile, Val in mitochondrial inner membrane and cross-references RHEA:70943, RHEA:73011, RHEA:29703.", "equation": "Leu, Ile, Val → Leu, Ile, Val", "inputs": [ { "node_id": "R-ALL-508182", "reactome_id": "R-ALL-508182", "label": "Leu, Ile, Val", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-508190", "reactome_id": "R-ALL-508190", "label": "Leu, Ile, Val", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "branched-chain amino acid transmembrane transporter activity of SLC25A44 [mitochondrial inner membrane]", "activity_id": "9672794", "ec_numbers": null, "accessions": [ "Q96H78" ] } ], "citations": [ { "id": "PMID:31435015", "title": "BCAA catabolism in brown fat controls energy homeostasis through SLC25A44", "journal": "Nature", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31435015/" } ], "x": 6521.333333333333, "y": 1541.3055555555557, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9677320", "label": "PTGS1 dimer binds PTGS1 Inhibitors", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9677320", "summary": "PTGS1 dimer binds PTGS1 Inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PTGS1 dimer, PTGS1 Inhibitors to PTGS1 dimer:PTGS1 Inhibitors in cytosol · endoplasmic reticulum membrane.", "equation": "PTGS1 dimer + PTGS1 Inhibitors → PTGS1 dimer:PTGS1 Inhibitors", "inputs": [ { "node_id": "R-HSA-428986", "reactome_id": "R-HSA-428986", "label": "PTGS1 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9677343", "reactome_id": "R-ALL-9677343", "label": "PTGS1 Inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9677354", "reactome_id": "R-HSA-9677354", "label": "PTGS1 dimer:PTGS1 Inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:5284360", "title": "Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs", "journal": "Nature New Biol.", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/5284360/" }, { "id": "PMID:9219313", "title": "Mechanism of action of aspirin-like drugs", "journal": "Semin. Arthritis Rheum.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9219313/" }, { "id": "PMID:9626023", "title": "Cyclooxygenase-1 and cyclooxygenase-2 selectivity of widely used nonsteroidal anti-inflammatory drugs", "journal": "Am. J. Med.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9626023/" }, { "id": "PMID:10377455", "title": "Nonsteroid drug selectivities for cyclo-oxygenase-1 rather than cyclo-oxygenase-2 are associated with human gastrointestinal toxicity: a full in vitro analysis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10377455/" } ], "x": 868.6666666666667, "y": 1748.7543859649122, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9677917", "label": "MAOA inhibitors bind MAOA:FAD", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9677917", "summary": "MAOA inhibitors bind MAOA:FAD is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects MAOA:FAD, MAOA Inhibitors to MAOA:FAD:MAOA Inhibitors in cytosol · mitochondrial outer membrane.", "equation": "MAOA:FAD + MAOA Inhibitors → MAOA:FAD:MAOA Inhibitors", "inputs": [ { "node_id": "R-HSA-141332", "reactome_id": "R-HSA-141332", "label": "MAOA:FAD", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9677898", "reactome_id": "R-ALL-9677898", "label": "MAOA Inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9677899", "reactome_id": "R-HSA-9677899", "label": "MAOA:FAD:MAOA Inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:21200377", "title": "Inhibition of monoamine oxidase activity by antidepressants and mood stabilizers", "journal": "Neuro Endocrinol Lett", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21200377/" }, { "id": "PMID:18426226", "title": "Structural and mechanistic studies of arylalkylhydrazine inhibition of human monoamine oxidases A and B", "journal": "Biochemistry", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18426226/" }, { "id": "PMID:21680183", "title": "Pyrazoline based MAO inhibitors: synthesis, biological evaluation and SAR studies", "journal": "Bioorg. Med. Chem. Lett.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21680183/" }, { "id": "PMID:15110846", "title": "Fluorinated phenylcyclopropylamines. Part 3: Inhibition of monoamine oxidase A and B", "journal": "Bioorg. Med. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15110846/" }, { "id": "PMID:8875133", "title": "Moclobemide. An update of its pharmacological properties and therapeutic use", "journal": "Drugs", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8875133/" }, { "id": "PMID:26316187", "title": "Monoamine Oxidase-A Occupancy by Moclobemide and Phenelzine: Implications for the Development of Monoamine Oxidase Inhibitors", "journal": "Int J Neuropsychopharmacol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26316187/" } ], "x": 7740, "y": 3172.9313725490197, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9677929", "label": "MAOB inhibitors bind MAOB:FAD", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9677929", "summary": "MAOB inhibitors bind MAOB:FAD is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects MAOB:FAD, MAOB Inhibitors to MAOB:FAD:MAOB Inhibitors in cytosol · mitochondrial outer membrane.", "equation": "MAOB:FAD + MAOB Inhibitors → MAOB:FAD:MAOB Inhibitors", "inputs": [ { "node_id": "R-HSA-141326", "reactome_id": "R-HSA-141326", "label": "MAOB:FAD", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9677913", "reactome_id": "R-ALL-9677913", "label": "MAOB Inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9677922", "reactome_id": "R-HSA-9677922", "label": "MAOB:FAD:MAOB Inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial outer membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:21680183", "title": "Pyrazoline based MAO inhibitors: synthesis, biological evaluation and SAR studies", "journal": "Bioorg. Med. Chem. Lett.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21680183/" }, { "id": "PMID:21377879", "title": "Development of selective and reversible pyrazoline based MAO-B inhibitors: virtual screening, synthesis and biological evaluation", "journal": "Bioorg. Med. Chem. Lett.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21377879/" }, { "id": "PMID:15110846", "title": "Fluorinated phenylcyclopropylamines. Part 3: Inhibition of monoamine oxidase A and B", "journal": "Bioorg. Med. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15110846/" }, { "id": "PMID:15974574", "title": "Design, synthesis, and biological activities of pyrrolylethanoneamine derivatives, a novel class of monoamine oxidases inhibitors", "journal": "J. Med. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15974574/" }, { "id": "PMID:24139167", "title": "1,3-Dialkyl-substituted tetrahydropyrimido[1,2-f]purine-2,4-diones as multiple target drugs for the potential treatment of neurodegenerative diseases", "journal": "Bioorg. Med. Chem.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24139167/" }, { "id": "PMID:11159700", "title": "Rasagiline [N-propargyl-1R(+)-aminoindan], a selective and potent inhibitor of mitochondrial monoamine oxidase B", "journal": "Br. J. Pharmacol.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11159700/" } ], "x": 8018.666666666667, "y": 3172.9313725490197, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9678749", "label": "IMPDH tetramers bind IMPDH inhibitors", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9678749", "summary": "IMPDH tetramers bind IMPDH inhibitors is a Reactome release 97 curated reaction event. The source record connects IMPDH tetramers, IMPDH inhibitors to IMPDH tetramers:IMPDH inhibitors in cytosol.", "equation": "IMPDH tetramers + IMPDH inhibitors → IMPDH tetramers:IMPDH inhibitors", "inputs": [ { "node_id": "R-HSA-500309", "reactome_id": "R-HSA-500309", "label": "IMPDH tetramers", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-9678719", "reactome_id": "R-ALL-9678719", "label": "IMPDH inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9679104", "reactome_id": "R-HSA-9679104", "label": "IMPDH tetramers:IMPDH inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:1967654", "title": "Synthesis and immunosuppressive activity of some side-chain variants of mycophenolic acid", "journal": "J. Med. Chem.", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/1967654/" }, { "id": "PMID:14529546", "title": "Thioguanine, mercaptopurine: their analogs and nucleosides as antimetabolites", "journal": "Curr. Pharm. Des.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14529546/" }, { "id": "PMID:18609073", "title": "IMP dehydrogenase basal activity in MOLT-4 human leukaemia cells is altered by mycophenolic acid and 6-thioguanosine", "journal": "Scand. J. Clin. Lab. Invest.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18609073/" }, { "id": "PMID:21320556", "title": "Mechanism of action of ribavirin in a novel hepatitis C virus replication cell system", "journal": "Virus Res.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21320556/" }, { "id": "PMID:21960835", "title": "Mechanism of action of ribavirin in the treatment of chronic hepatitis C", "journal": "Gastroenterol Hepatol (N Y)", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/21960835/" } ], "x": 2660, "y": 2943.15625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9678766", "label": "CYP3A4 binds CYP3A4 inhibitors", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9678766", "summary": "CYP3A4 binds CYP3A4 inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CYP3A4, CYP3A4 inhibitors to CYP3A4:CYP3A4 inhibitors in cytosol · endoplasmic reticulum membrane.", "equation": "CYP3A4 + CYP3A4 inhibitors → CYP3A4:CYP3A4 inhibitors", "inputs": [ { "node_id": "R-HSA-52639", "reactome_id": "R-HSA-52639", "label": "CYP3A4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9679016", "reactome_id": "R-ALL-9679016", "label": "CYP3A4 inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9679000", "reactome_id": "R-HSA-9679000", "label": "CYP3A4:CYP3A4 inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10445380", "title": "Nefazodone, meta-chlorophenylpiperazine, and their metabolites in vitro: cytochromes mediating transformation, and P450-3A4 inhibitory actions", "journal": "Psychopharmacology (Berl.)", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10445380/" }, { "id": "PMID:25923589", "title": "Ritonavir is the best alternative to ketoconazole as an index inhibitor of cytochrome P450-3A in drug-drug interaction studies", "journal": "Br J Clin Pharmacol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25923589/" }, { "id": "PMID:11422004", "title": "Inhibitory effects of verapamil and diltiazem on simvastatin metabolism in human liver microsomes", "journal": "Br J Clin Pharmacol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11422004/" }, { "id": "PMID:23616950", "title": "Cobicistat, a pharmacoenhancer for HIV treatments", "journal": "Drugs Today", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23616950/" }, { "id": "PMID:24900196", "title": "Cobicistat (GS-9350): A Potent and Selective Inhibitor of Human CYP3A as a Novel Pharmacoenhancer", "journal": "ACS Med Chem Lett", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/24900196/" } ], "x": 7461.333333333333, "y": 3180.5588235294117, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9680865", "label": "CYP3A4, CYP2B6 bind ritonavir", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9680865", "summary": "CYP3A4, CYP2B6 bind ritonavir is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CYP3A4, CYP2B6, ritonavir to CYP3A4,CYP2B6:ritinovir in cytosol · endoplasmic reticulum membrane.", "equation": "CYP3A4, CYP2B6 + ritonavir → CYP3A4,CYP2B6:ritinovir", "inputs": [ { "node_id": "R-HSA-9680941", "reactome_id": "R-HSA-9680941", "label": "CYP3A4, CYP2B6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-9679091", "reactome_id": "R-ALL-9679091", "label": "ritonavir", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9680864", "reactome_id": "R-HSA-9680864", "label": "CYP3A4,CYP2B6:ritinovir", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:18285471", "title": "Rapid clinical induction of hepatic cytochrome P4502B6 activity by ritonavir", "journal": "Antimicrob. Agents Chemother.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18285471/" }, { "id": "PMID:25923589", "title": "Ritonavir is the best alternative to ketoconazole as an index inhibitor of cytochrome P450-3A in drug-drug interaction studies", "journal": "Br J Clin Pharmacol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25923589/" }, { "id": "PMID:15523003", "title": "Mechanism-based inactivation of CYP3A by HIV protease inhibitors", "journal": "J. Pharmacol. Exp. Ther.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15523003/" }, { "id": "PMID:32152082", "title": "Compounds with therapeutic potential against novel respiratory 2019 coronavirus", "journal": "Antimicrob. Agents Chemother.", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32152082/" }, { "id": "PMID:32210741", "title": "Unrevealing sequence and structural features of novel coronavirus using in silico approaches: The main protease as molecular target", "journal": "EXCLI J", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32210741/" }, { "id": "PMID:32187464", "title": "A Trial of Lopinavir-Ritonavir in Adults Hospitalized with Severe Covid-19", "journal": "N. Engl. J. Med.", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32187464/" } ], "x": 7740, "y": 3180.5588235294117, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9693722", "label": "Unknown sepiapterin synthase transforms PTHP to sepiapterin", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9693722", "summary": "Unknown sepiapterin synthase transforms PTHP to sepiapterin is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PTHP to sepiapterin in cytosol.", "equation": "PTHP → sepiapterin", "inputs": [ { "node_id": "R-ALL-1474179", "reactome_id": "R-ALL-1474179", "label": "PTHP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17804", "formula": "C9H11N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1497811", "reactome_id": "R-ALL-1497811", "label": "sepiapterin", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:194527", "formula": "C9H11N5O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity of sepiapterin synthase [cytosol]", "activity_id": "9693719", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:21550412", "title": "Synthesis and recycling of tetrahydrobiopterin in endothelial function and vascular disease", "journal": "Nitric Oxide", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21550412/" } ], "x": 5008.666666666667, "y": 3622.375, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9695890", "label": "SPNS2,MFSD2B transport S1P from cytosol to extracellular region", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · extracellular region · plasma membrane", "compartment_ids": [ "GO:0005829", "GO:0005576", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9695890", "summary": "SPNS2,MFSD2B transport S1P from cytosol to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects S1P to S1P in cytosol · extracellular region · plasma membrane.", "equation": "S1P → S1P", "inputs": [ { "node_id": "R-ALL-5279322", "reactome_id": "R-ALL-5279322", "label": "S1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:76941", "formula": "C3H8NO5PR", "compartment": "cytoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9843733", "reactome_id": "R-ALL-9843733", "label": "S1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76941", "formula": "C3H8NO5PR", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sphingolipid intramembrane carrier activity of SPNS2,MFSD2B [plasma membrane]", "activity_id": "9695894", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:23180825", "title": "Spns2, a transporter of phosphorylated sphingoid bases, regulates their blood and lymph levels, and the lymphatic network", "journal": "FASEB J.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23180825/" }, { "id": "PMID:30655317", "title": "New insights into functions of the sphingosine-1-phosphate transporter SPNS2", "journal": "J Lipid Res", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30655317/" }, { "id": "PMID:37224812", "title": "Structural and functional insights into Spns2-mediated transport of sphingosine-1-phosphate", "journal": "Cell", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37224812/" }, { "id": "PMID:37390971", "title": "An erythrocyte-centric view on the MFSD2B sphingosine-1-phosphate transporter", "journal": "Pharmacol Ther", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37390971/" }, { "id": "PMID:29563527", "title": "MFSD2B is a sphingosine 1-phosphate transporter in erythroid cells", "journal": "Sci Rep", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29563527/" } ], "x": 3321.3333333333335, "y": 1854.673076923077, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9695917", "label": "SPHK2 binds SPHK2 inhibitors", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9695917", "summary": "SPHK2 binds SPHK2 inhibitors is a Reactome release 97 curated reaction event. The source record connects SPHK2, SPHK2 inhibitors to SPHK2:SPHK2 inhibitors in cytosol.", "equation": "SPHK2 + SPHK2 inhibitors → SPHK2:SPHK2 inhibitors", "inputs": [ { "node_id": "R-HSA-428236", "reactome_id": "R-HSA-428236", "label": "SPHK2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9695954", "reactome_id": "R-ALL-9695954", "label": "SPHK2 inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "CandidateSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9695910", "reactome_id": "R-HSA-9695910", "label": "SPHK2:SPHK2 inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20061445", "title": "Pharmacology and antitumor activity of ABC294640, a selective inhibitor of sphingosine kinase-2", "journal": "J. Pharmacol. Exp. Ther.", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20061445/" }, { "id": "PMID:22970244", "title": "Characterization of isoenzyme-selective inhibitors of human sphingosine kinases", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22970244/" }, { "id": "PMID:21620961", "title": "(R)-FTY720 methyl ether is a specific sphingosine kinase 2 inhibitor: Effect on sphingosine kinase 2 expression in HEK 293 cells and actin rearrangement and survival of MCF-7 breast cancer cells", "journal": "Cell. Signal.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21620961/" }, { "id": "PMID:25788259", "title": "A selective ATP-competitive sphingosine kinase inhibitor demonstrates anti-cancer properties", "journal": "Oncotarget", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25788259/" }, { "id": "PMID:31895563", "title": "Discovery of a Small Side Cavity in Sphingosine Kinase 2 that Enhances Inhibitor Potency and Selectivity", "journal": "J. Med. Chem.", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/31895563/" }, { "id": "PMID:31783527", "title": "Emerging Connections of S1P-Metabolizing Enzymes with Host Defense and Immunity During Virus Infections", "journal": "Viruses", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31783527/" } ], "x": 3321.3333333333335, "y": 1899.5576923076924, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9695949", "label": "SPHK2 phosphorylates sphingoid", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:51496" ], "source_url": "https://reactome.org/content/detail/R-HSA-9695949", "summary": "SPHK2 phosphorylates sphingoid is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATP, SPG to ADP, H+, S1P in cytosol · endoplasmic reticulum membrane and cross-references RHEA:51496.", "equation": "ATP + SPG → ADP + H+ + S1P", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428682", "reactome_id": "R-ALL-428682", "label": "SPG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:84410", "formula": "C3H9NO2R", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-5279322", "reactome_id": "R-ALL-5279322", "label": "S1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76941", "formula": "C3H8NO5PR", "compartment": "cytoplasm", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lipid kinase activity of SPHK2 [cytosol]", "activity_id": "9695919", "ec_numbers": null, "accessions": [ "Q9NRA0" ] } ], "citations": [ { "id": "PMID:10751414", "title": "Molecular cloning and functional characterization of a novel mammalian sphingosine kinase type 2 isoform", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10751414/" }, { "id": "PMID:16118219", "title": "SphK1 and SphK2, sphingosine kinase isoenzymes with opposing functions in sphingolipid metabolism", "journal": "J. Biol. Chem.", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16118219/" }, { "id": "PMID:28415564", "title": "Mammalian sphingosine kinase (SphK) isoenzymes and isoform expression: challenges for SphK as an oncotarget", "journal": "Oncotarget", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28415564/" }, { "id": "PMID:21864225", "title": "The compartmentalization and translocation of the sphingosine kinases: mechanisms and functions in cell signaling and sphingolipid metabolism", "journal": "Crit Rev Biochem Mol Biol", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21864225/" } ], "x": 3878.666666666667, "y": 1570.4038461538462, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9705584", "label": "HMGCR dimer binds statins", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9705584", "summary": "HMGCR dimer binds statins is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects HMGCR dimer, statins to HMGCR dimer:statins in cytosol · endoplasmic reticulum membrane.", "equation": "HMGCR dimer + statins → HMGCR dimer:statins", "inputs": [ { "node_id": "R-HSA-191277", "reactome_id": "R-HSA-191277", "label": "HMGCR dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9705575", "reactome_id": "R-ALL-9705575", "label": "statins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9705595", "reactome_id": "R-HSA-9705595", "label": "HMGCR dimer:statins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16128575", "title": "Binding thermodynamics of statins to HMG-CoA reductase", "journal": "Biochemistry", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16128575/" }, { "id": "PMID:32859023", "title": "An Atomic-Level Perspective of HMG-CoA-Reductase: The Target Enzyme to Treat Hypercholesterolemia", "journal": "Molecules", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32859023/" }, { "id": "PMID:12486413", "title": "Structural mechanism for statin inhibition of 3-hydroxy-3-methylglutaryl coenzyme A reductase", "journal": "Am Heart J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12486413/" }, { "id": "PMID:11349148", "title": "Structural mechanism for statin inhibition of HMG-CoA reductase", "journal": "Science", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11349148/" } ], "x": 2660, "y": 2388.0333333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9705713", "label": "SRD5A2 dehydrogenates TEST to DHTEST", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:50822" ], "source_url": "https://reactome.org/content/detail/R-HSA-9705713", "summary": "SRD5A2 dehydrogenates TEST to DHTEST is a Reactome release 97 curated reaction event. The source record connects TEST, NADPH, H+ to DHTEST, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:50822.", "equation": "TEST + NADPH + H+ → DHTEST + NADP+", "inputs": [ { "node_id": "R-ALL-193057", "reactome_id": "R-ALL-193057", "label": "TEST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17347", "formula": "C19H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-469662", "reactome_id": "R-ALL-469662", "label": "DHTEST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16330", "formula": "C19H30O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-oxo-5-alpha-steroid 4-dehydrogenase activity of SRD5A2 [endoplasmic reticulum membrane]", "activity_id": "9705712", "ec_numbers": [ "1.3.99.5" ], "accessions": [ "P31213" ] } ], "citations": [ { "id": "PMID:1944596", "title": "Deletion of steroid 5 alpha-reductase 2 gene in male pseudohermaphroditism", "journal": "Nature", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1944596/" }, { "id": "PMID:9208814", "title": "Male pseudohermaphroditism resulting from a novel mutation in the human steroid 5 alpha-reductase type 2 gene (SRD5A2)", "journal": "Mol Pathol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9208814/" }, { "id": "PMID:9536209", "title": "A critical review of the origin and control of adrenal androgens", "journal": "Baillieres Clin Obstet Gynaecol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9536209/" } ], "x": 5008.666666666667, "y": 1618.4621212121212, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9705714", "label": "SRD5A3 dehydrogenates TEST to DHTEST", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [ "RHEA:50822" ], "source_url": "https://reactome.org/content/detail/R-HSA-9705714", "summary": "SRD5A3 dehydrogenates TEST to DHTEST is a Reactome release 97 curated reaction event. The source record connects TEST, NADPH, H+ to DHTEST, NADP+ in cytosol · endoplasmic reticulum membrane and cross-references RHEA:50822.", "equation": "TEST + NADPH + H+ → DHTEST + NADP+", "inputs": [ { "node_id": "R-ALL-193057", "reactome_id": "R-ALL-193057", "label": "TEST", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17347", "formula": "C19H28O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-469662", "reactome_id": "R-ALL-469662", "label": "DHTEST", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16330", "formula": "C19H30O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-oxo-5-alpha-steroid 4-dehydrogenase activity of SRD5A3 [endoplasmic reticulum membrane]", "activity_id": "9705710", "ec_numbers": [ "1.3.99.5" ], "accessions": [ "Q9H8P0" ] } ], "citations": [ { "id": "PMID:17986282", "title": "Novel 5 alpha-steroid reductase (SRD5A3, type-3) is overexpressed in hormone-refractory prostate cancer", "journal": "Cancer Sci", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17986282/" }, { "id": "PMID:9536209", "title": "A critical review of the origin and control of adrenal androgens", "journal": "Baillieres Clin Obstet Gynaecol", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9536209/" } ], "x": 4451.333333333333, "y": 1624.3560606060605, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9705794", "label": "SRD5A2 binds SRD5A2 inhibitors", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9705794", "summary": "SRD5A2 binds SRD5A2 inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects SRD5A2, SRD5A2 inhibitors to SRD5A2:SRD5A2 inhibitors in cytosol · endoplasmic reticulum membrane.", "equation": "SRD5A2 + SRD5A2 inhibitors → SRD5A2:SRD5A2 inhibitors", "inputs": [ { "node_id": "R-HSA-469664", "reactome_id": "R-HSA-469664", "label": "SRD5A2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9705798", "reactome_id": "R-ALL-9705798", "label": "SRD5A2 inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9705766", "reactome_id": "R-HSA-9705766", "label": "SRD5A2:SRD5A2 inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:3783591", "title": "Azasteroids: structure-activity relationships for inhibition of 5 alpha-reductase and of androgen receptor binding", "journal": "J Med Chem", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3783591/" }, { "id": "PMID:9871428", "title": "A model for the turnover of dihydrotestosterone in the presence of the irreversible 5 alpha-reductase inhibitors GI198745 and finasteride", "journal": "Clin Pharmacol Ther", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9871428/" }, { "id": "PMID:19936164", "title": "Therapeutic options in the treatment of benign prostatic hyperplasia", "journal": "Patient Prefer Adherence", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19936164/" }, { "id": "PMID:25961201", "title": "Human type 3 5α-reductase is expressed in peripheral tissues at higher levels than types 1 and 2 and its activity is potently inhibited by finasteride and dutasteride", "journal": "Horm Mol Biol Clin Investig", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/25961201/" }, { "id": "PMID:18318566", "title": "Dutasteride: a review of its use in the management of prostate disorders", "journal": "Drugs", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18318566/" }, { "id": "PMID:1279965", "title": "Finasteride for benign prostatic hyperplasia", "journal": "Am Fam Physician", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1279965/" } ], "x": 4730, "y": 1830.6439393939395, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9707186", "label": "ALOX5 binds ALOX5 inhibitors", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "nuclear envelope · cytosol", "compartment_ids": [ "GO:0005635", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9707186", "summary": "ALOX5 binds ALOX5 inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ALOX5:ALOX5AP:LTC4S, ALOX5 inhibitors to ALOX5:ALOX5 inhibitors in nuclear envelope · cytosol.", "equation": "ALOX5:ALOX5AP:LTC4S + ALOX5 inhibitors → ALOX5:ALOX5 inhibitors", "inputs": [ { "node_id": "R-HSA-2318764", "reactome_id": "R-HSA-2318764", "label": "ALOX5:ALOX5AP:LTC4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nuclear envelope", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9707237", "reactome_id": "R-ALL-9707237", "label": "ALOX5 inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9707188", "reactome_id": "R-HSA-9707188", "label": "ALOX5:ALOX5 inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nuclear envelope", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:1848634", "title": "5-lipoxygenase inhibitory activity of zileuton", "journal": "J Pharmacol Exp Ther", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1848634/" }, { "id": "PMID:20436887", "title": "Zileuton, a new efficient and safe systemic anti-acne drug", "journal": "Dermatoendocrinol", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/20436887/" }, { "id": "PMID:15604543", "title": "Zileuton, an oral 5-lipoxygenase inhibitor, directly reduces sebum production", "journal": "Dermatology", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15604543/" }, { "id": "PMID:29133059", "title": "Recent advances in the search for novel 5-lipoxygenase inhibitors for the treatment of asthma", "journal": "Eur J Med Chem", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29133059/" }, { "id": "PMID:27046942", "title": "Maximum Time of the Effect of Antileukotriene - Zileuton in Treatment of Patients with Bronchial Asthma", "journal": "Acta Inform Med", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27046942/" }, { "id": "PMID:3020588", "title": "Meclofenamate sodium is an inhibitor of both the 5-lipoxygenase and cyclooxygenase pathways of the arachidonic acid cascade in vitro", "journal": "Prostaglandins Leukot Med", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3020588/" } ], "x": 311.33333333333337, "y": 1751.7719298245613, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9707353", "label": "CYP19A1 binds Aromatase inhibitors", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "steroids" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9707353", "summary": "CYP19A1 binds Aromatase inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CYP19A1, Aromatase inhibitors to CYP19A1:Aromatase inhibitors in cytosol · endoplasmic reticulum membrane.", "equation": "CYP19A1 + Aromatase inhibitors → CYP19A1:Aromatase inhibitors", "inputs": [ { "node_id": "R-HSA-193137", "reactome_id": "R-HSA-193137", "label": "CYP19A1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9707314", "reactome_id": "R-ALL-9707314", "label": "Aromatase inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9707321", "reactome_id": "R-HSA-9707321", "label": "CYP19A1:Aromatase inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:7336466", "title": "Inactivation of aromatase in vitro by 4-hydroxy-4-androstene-3,17-dione and 4-acetoxy-4-androstene-3,17-dione and sustained effects in vivo", "journal": "Steroids", "year": 1981, "url": "https://pubmed.ncbi.nlm.nih.gov/7336466/" }, { "id": "PMID:2149502", "title": "Highly selective inhibition of estrogen biosynthesis by CGS 20267, a new non-steroidal aromatase inhibitor", "journal": "J Steroid Biochem Mol Biol", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2149502/" }, { "id": "PMID:11895893", "title": "Approval summary: letrozole in the treatment of postmenopausal women with advanced breast cancer", "journal": "Clin Cancer Res", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11895893/" }, { "id": "PMID:12404296", "title": "An overview of the pharmacology and pharmacokinetics of the newer generation aromatase inhibitors anastrozole, letrozole, and exemestane", "journal": "Cancer", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12404296/" }, { "id": "PMID:16332571", "title": "HPMA copolymer-aminoglutethimide conjugates inhibit aromatase in MCF-7 cell lines", "journal": "J Drug Target", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16332571/" }, { "id": "PMID:1825337", "title": "Fadrozole hydrochloride: a potent, selective, nonsteroidal inhibitor of aromatase for the treatment of estrogen-dependent disease", "journal": "J Med Chem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1825337/" } ], "x": 5008.666666666667, "y": 1830.6439393939395, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9708759", "label": "DHODH:FMN binds DHODH inhibitors", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-biosynthesis" ], "compartment": "mitochondrial inner membrane · cytosol", "compartment_ids": [ "GO:0005743", "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9708759", "summary": "DHODH:FMN binds DHODH inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects DHODH:FMN, DHODH inhibitors to DHODH:FMN:DHODH inhibitors in mitochondrial inner membrane · cytosol.", "equation": "DHODH:FMN + DHODH inhibitors → DHODH:FMN:DHODH inhibitors", "inputs": [ { "node_id": "R-HSA-73534", "reactome_id": "R-HSA-73534", "label": "DHODH:FMN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9708729", "reactome_id": "R-ALL-9708729", "label": "DHODH inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "CandidateSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9708760", "reactome_id": "R-HSA-9708760", "label": "DHODH:FMN:DHODH inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:8573583", "title": "The immunosuppressive metabolite of leflunomide is a potent inhibitor of human dihydroorotate dehydrogenase", "journal": "Biochemistry", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8573583/" }, { "id": "PMID:11053058", "title": "Leflunomide: mode of action in the treatment of rheumatoid arthritis", "journal": "Ann Rheum Dis", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11053058/" }, { "id": "PMID:21157651", "title": "Teriflunomide, an inhibitor of dihydroorotate dehydrogenase for the potential oral treatment of multiple sclerosis", "journal": "Curr Opin Investig Drugs", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/21157651/" }, { "id": "PMID:32754890", "title": "Novel and potent inhibitors targeting DHODH are broad-spectrum antivirals against RNA viruses including newly-emerged coronavirus SARS-CoV-2", "journal": "Protein Cell", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32754890/" }, { "id": "PMID:32735689", "title": "Dihydroorotate dehydrogenase inhibitors in SARS-CoV-2 infection", "journal": "Eur J Clin Invest", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32735689/" }, { "id": "PMID:32696396", "title": "A Small-Scale Medication of Leflunomide as a Treatment of COVID-19 in an Open-Label Blank-Controlled Clinical Trial", "journal": "Virol Sin", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32696396/" } ], "x": 2938.666666666667, "y": 2943.15625, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9709098", "label": "DHFR dimer reduces FOLA to DHF", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:31105" ], "source_url": "https://reactome.org/content/detail/R-HSA-9709098", "summary": "DHFR dimer reduces FOLA to DHF is a Reactome release 97 curated reaction event. The source record connects FOLA, NADPH, H+ to DHF, NADP+ in cytosol and cross-references RHEA:31105.", "equation": "FOLA + NADPH + H+ → DHF + NADP+", "inputs": [ { "node_id": "R-ALL-197965", "reactome_id": "R-ALL-197965", "label": "FOLA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62501", "formula": "C19H17N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-73604", "reactome_id": "R-ALL-73604", "label": "DHF", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57451", "formula": "C19H19N7O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "folate reductase activity of DHFR dimer [cytosol]", "activity_id": "9709122", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:6323448", "title": "The functional human dihydrofolate reductase gene", "journal": "J Biol Chem", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6323448/" }, { "id": "PMID:2248959", "title": "Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate", "journal": "Biochemistry", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2248959/" } ], "x": 4451.333333333333, "y": 2856.0454545454545, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9709109", "label": "DHFR dimer binds DHFR inhibitors", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "folate-pterines", "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9709109", "summary": "DHFR dimer binds DHFR inhibitors is a Reactome release 97 curated reaction event. The source record connects DHFR dimer, DHFR inhibitors to DHFR dimer:DHFR inhibitors in cytosol.", "equation": "DHFR dimer + DHFR inhibitors → DHFR dimer:DHFR inhibitors", "inputs": [ { "node_id": "R-HSA-1497822", "reactome_id": "R-HSA-1497822", "label": "DHFR dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9709140", "reactome_id": "R-ALL-9709140", "label": "DHFR inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9709105", "reactome_id": "R-HSA-9709105", "label": "DHFR dimer:DHFR inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:32423175", "title": "Overview of Dual-Acting Drug Methotrexate in Different Neurological Diseases, Autoimmune Pathologies and Cancers", "journal": "Int J Mol Sci", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32423175/" }, { "id": "PMID:32658475", "title": "The Structural Basis for Nonsteroidal Anti-Inflammatory Drug Inhibition of Human Dihydrofolate Reductase", "journal": "J Med Chem", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32658475/" }, { "id": "PMID:17308042", "title": "Pemetrexed: biochemical and cellular pharmacology, mechanisms, and clinical applications", "journal": "Mol Cancer Ther", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17308042/" }, { "id": "PMID:33053560", "title": "Irinotecan and Gemcitabine as Second-Line Treatment in Patients with Malignant Pleural Mesothelioma following Platinum plus Pemetrexed Chemotherapy: A Retrospective Study", "journal": "Oncology", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33053560/" }, { "id": "PMID:10091649", "title": "Structure and dynamics in solution of the complex of Lactobacillus casei dihydrofolate reductase with the new lipophilic antifolate drug trimetrexate", "journal": "Protein Sci", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10091649/" }, { "id": "PMID:19424049", "title": "Trimetrexate and folinic acid: a valuable salvage option for Pneumocystis jirovecii pneumonia", "journal": "AIDS", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19424049/" } ], "x": 5008.666666666667, "y": 3138.9545454545455, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9716836", "label": "PTGS2 dimer binds PTGS inhibitors", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9716836", "summary": "PTGS2 dimer binds PTGS inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PTGS2 dimer, PTGS inhibitors to PTGS2 dimer:PTGS inhibitors in cytosol · endoplasmic reticulum membrane.", "equation": "PTGS2 dimer + PTGS inhibitors → PTGS2 dimer:PTGS inhibitors", "inputs": [ { "node_id": "R-HSA-140491", "reactome_id": "R-HSA-140491", "label": "PTGS2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9716837", "reactome_id": "R-ALL-9716837", "label": "PTGS inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9716835", "reactome_id": "R-HSA-9716835", "label": "PTGS2 dimer:PTGS inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10966456", "title": "Cyclooxygenases: structural, cellular, and molecular biology", "journal": "Annu Rev Biochem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10966456/" }, { "id": "PMID:8901870", "title": "Flexibility of the NSAID binding site in the structure of human cyclooxygenase-2", "journal": "Nat. Struct. Biol.", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8901870/" }, { "id": "PMID:21467029", "title": "Human cyclooxygenase-2 is a sequence homodimer that functions as a conformational heterodimer", "journal": "J. Biol. Chem.", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21467029/" }, { "id": "PMID:28710775", "title": "Cyclooxygenase Inhibition: Pain, Inflammation, and the Cardiovascular System", "journal": "Clin Pharmacol Ther", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28710775/" } ], "x": 590, "y": 1751.7719298245613, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9716838", "label": "PTGS1,2 dimers bind PTGS Inhibitors", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0005829", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9716838", "summary": "PTGS1,2 dimers bind PTGS Inhibitors is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PTGS1 dimer, PTGS inhibitors, PTGS2 dimer to PTGS1 dimer:PTGS Inhibitors, PTGS2 dimer:PTGS inhibitors in cytosol · endoplasmic reticulum membrane.", "equation": "PTGS1 dimer + PTGS inhibitors + PTGS2 dimer → PTGS1 dimer:PTGS Inhibitors + PTGS2 dimer:PTGS inhibitors", "inputs": [ { "node_id": "R-HSA-428986", "reactome_id": "R-HSA-428986", "label": "PTGS1 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9716837", "reactome_id": "R-ALL-9716837", "label": "PTGS inhibitors", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-140491", "reactome_id": "R-HSA-140491", "label": "PTGS2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9716834", "reactome_id": "R-HSA-9716834", "label": "PTGS1 dimer:PTGS Inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9716835", "reactome_id": "R-HSA-9716835", "label": "PTGS2 dimer:PTGS inhibitors", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:5284360", "title": "Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs", "journal": "Nature New Biol.", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/5284360/" }, { "id": "PMID:9219313", "title": "Mechanism of action of aspirin-like drugs", "journal": "Semin. Arthritis Rheum.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9219313/" }, { "id": "PMID:9626023", "title": "Cyclooxygenase-1 and cyclooxygenase-2 selectivity of widely used nonsteroidal anti-inflammatory drugs", "journal": "Am. J. Med.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9626023/" }, { "id": "PMID:10377455", "title": "Nonsteroid drug selectivities for cyclo-oxygenase-1 rather than cyclo-oxygenase-2 are associated with human gastrointestinal toxicity: a full in vitro analysis", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10377455/" }, { "id": "PMID:28710775", "title": "Cyclooxygenase Inhibition: Pain, Inflammation, and the Cardiovascular System", "journal": "Clin Pharmacol Ther", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28710775/" } ], "x": 868.6666666666667, "y": 1751.7719298245613, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9717830", "label": "GGPS1 hexamer transfers IPPP to GPP", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19362" ], "source_url": "https://reactome.org/content/detail/R-HSA-9717830", "summary": "GGPS1 hexamer transfers IPPP to GPP is a Reactome release 97 curated reaction event. The source record connects GPP, IPPP to FAPP, PPi in cytosol and cross-references RHEA:19362.", "equation": "GPP + IPPP → FAPP + PPi", "inputs": [ { "node_id": "R-ALL-191409", "reactome_id": "R-ALL-191409", "label": "GPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58057", "formula": "C10H17O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-191362", "reactome_id": "R-ALL-191362", "label": "IPPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:128769", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-191385", "reactome_id": "R-ALL-191385", "label": "FAPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:175763", "formula": "C15H25O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "(2E,6E)-farnesyl diphosphate synthase activity of holo-GGPS1 hexamer [cytosol]", "activity_id": "9717839", "ec_numbers": [ "2.5.1.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:10026212", "title": "Human geranylgeranyl diphosphate synthase. cDNA cloning and expression.", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10026212/" }, { "id": "PMID:9741684", "title": "Human geranylgeranyl diphosphate synthase: isolation of the cDNA, chromosomal mapping and tissue expression", "journal": "J Lipid Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9741684/" }, { "id": "PMID:16684881", "title": "The molecular mechanism of nitrogen-containing bisphosphonates as antiosteoporosis drugs", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16684881/" } ], "x": 2381.3333333333335, "y": 2232.4333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9717834", "label": "GGPS1 hexamer transfers IPPP to DMAPP", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:22409" ], "source_url": "https://reactome.org/content/detail/R-HSA-9717834", "summary": "GGPS1 hexamer transfers IPPP to DMAPP is a Reactome release 97 curated reaction event. The source record connects DMAPP, IPPP to GPP, PPi in cytosol and cross-references RHEA:22409.", "equation": "DMAPP + IPPP → GPP + PPi", "inputs": [ { "node_id": "R-ALL-191324", "reactome_id": "R-ALL-191324", "label": "DMAPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57623", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-191362", "reactome_id": "R-ALL-191362", "label": "IPPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:128769", "formula": "C5H9O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-191409", "reactome_id": "R-ALL-191409", "label": "GPP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58057", "formula": "C10H17O7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "dimethylallyltranstransferase activity of holo-GGPS1 hexamer [cytosol]", "activity_id": "9717831", "ec_numbers": [ "2.5.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:10026212", "title": "Human geranylgeranyl diphosphate synthase. cDNA cloning and expression.", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10026212/" }, { "id": "PMID:9741684", "title": "Human geranylgeranyl diphosphate synthase: isolation of the cDNA, chromosomal mapping and tissue expression", "journal": "J Lipid Res", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9741684/" }, { "id": "PMID:16684881", "title": "The molecular mechanism of nitrogen-containing bisphosphonates as antiosteoporosis drugs", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16684881/" }, { "id": "PMID:33246356", "title": "Structure, catalysis, and inhibition mechanism of prenyltransferase", "journal": "IUBMB Life", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33246356/" } ], "x": 2660, "y": 2232.4333333333334, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9717841", "label": "holo-FDPS dimer binds NBPs", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9717841", "summary": "holo-FDPS dimer binds NBPs is a Reactome release 97 curated reaction event. The source record connects holo-FDPS dimer, NBPs to holo-FDPS dimer:NBPs in cytosol.", "equation": "holo-FDPS dimer + NBPs → holo-FDPS dimer:NBPs", "inputs": [ { "node_id": "R-HSA-981570", "reactome_id": "R-HSA-981570", "label": "holo-FDPS dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9717840", "reactome_id": "R-ALL-9717840", "label": "NBPs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9717836", "reactome_id": "R-HSA-9717836", "label": "holo-FDPS dimer:NBPs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:10620343", "title": "Alendronate is a specific, nanomolar inhibitor of farnesyl diphosphate synthase", "journal": "Arch. Biochem. Biophys.", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10620343/" }, { "id": "PMID:18937434", "title": "Design, synthesis, and biological evaluation of novel aminobisphosphonates possessing an in vivo antitumor activity through a gammadelta-T lymphocytes-mediated activation mechanism", "journal": "J. Med. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18937434/" }, { "id": "PMID:18327899", "title": "Structure-activity relationships among the nitrogen containing bisphosphonates in clinical use and other analogues: time-dependent inhibition of human farnesyl pyrophosphate synthase", "journal": "J. Med. Chem.", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18327899/" }, { "id": "PMID:11160603", "title": "Structure-activity relationships for inhibition of farnesyl diphosphate synthase in vitro and inhibition of bone resorption in vivo by nitrogen-containing bisphosphonates", "journal": "J. Pharmacol. Exp. Ther.", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11160603/" }, { "id": "PMID:21420384", "title": "Correlation between time-dependent inhibition of human farnesyl pyrophosphate synthase and blockade of mevalonate pathway by nitrogen-containing bisphosphonates in cultured cells", "journal": "Biochem Biophys Res Commun", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21420384/" }, { "id": "PMID:20394422", "title": "Synthesis, chiral high performance liquid chromatographic resolution and enantiospecific activity of a potent new geranylgeranyl transferase inhibitor, 2-hydroxy-3-imidazo[1,2-a]pyridin-3-yl-2-phosphonopropionic acid", "journal": "J Med Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20394422/" } ], "x": 2938.666666666667, "y": 2388.0333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9727347", "label": "XDH dehydrogenates hypoxanthine to form xanthine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:24671" ], "source_url": "https://reactome.org/content/detail/R-HSA-9727347", "summary": "XDH dehydrogenates hypoxanthine to form xanthine is a Reactome release 97 curated reaction event. The source record connects H2O, Hyp, NAD+ to H+, NADH, XAN in cytosol and cross-references RHEA:24671.", "equation": "H2O + Hyp + NAD+ → H+ + NADH + XAN", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113599", "reactome_id": "R-ALL-113599", "label": "Hyp", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17368", "formula": "C5H4N4O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30064", "reactome_id": "R-ALL-30064", "label": "XAN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17712", "formula": "C5H4N4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hypoxanthine dehydrogenase activity of XDH dimer [cytosol]", "activity_id": "9727348", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:23203137", "title": "Mutations associated with functional disorder of xanthine oxidoreductase and hereditary xanthinuria in humans", "journal": "Int J Mol Sci", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23203137/" }, { "id": "PMID:8670112", "title": "Cloning and expression in vitro of human xanthine dehydrogenase/oxidase", "journal": "Biochem J", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8670112/" }, { "id": "PMID:17301077", "title": "Human xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site: roles of active site residues in binding and activation of purine substrate", "journal": "J Biochem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17301077/" } ], "x": 2381.3333333333335, "y": 3088.7738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9727349", "label": "XDH dehydrogenates xanthine to form urate", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16670" ], "source_url": "https://reactome.org/content/detail/R-HSA-9727349", "summary": "XDH dehydrogenates xanthine to form urate is a Reactome release 97 curated reaction event. The source record connects H2O, NAD+, XAN to H+, NADH, urate in cytosol and cross-references RHEA:16670.", "equation": "H2O + NAD+ + XAN → H+ + NADH + urate", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30064", "reactome_id": "R-ALL-30064", "label": "XAN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17712", "formula": "C5H4N4O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-30034", "reactome_id": "R-ALL-30034", "label": "urate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17775", "formula": "C5H4N4O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "xanthine dehydrogenase activity of XDH dimer [cytosol]", "activity_id": "9727342", "ec_numbers": [ "1.17.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:23203137", "title": "Mutations associated with functional disorder of xanthine oxidoreductase and hereditary xanthinuria in humans", "journal": "Int J Mol Sci", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23203137/" }, { "id": "PMID:8670112", "title": "Cloning and expression in vitro of human xanthine dehydrogenase/oxidase", "journal": "Biochem J", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8670112/" }, { "id": "PMID:17301077", "title": "Human xanthine oxidase changes its substrate specificity to aldehyde oxidase type upon mutation of amino acid residues in the active site: roles of active site residues in binding and activation of purine substrate", "journal": "J Biochem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17301077/" } ], "x": 2660, "y": 3088.7738095238096, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9731228", "label": "NUDT1 hydrolyses 8-oxo-dATP to 8-oxo-dAMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:65397" ], "source_url": "https://reactome.org/content/detail/R-HSA-9731228", "summary": "NUDT1 hydrolyses 8-oxo-dATP to 8-oxo-dAMP is a Reactome release 97 curated reaction event. The source record connects 8-oxo-dATP(4-), H2O to 8-oxo-dAMP(2-), H+, PPi in cytosol and cross-references RHEA:65397.", "equation": "8-oxo-dATP(4-) + H2O → 8-oxo-dAMP(2-) + H+ + PPi", "inputs": [ { "node_id": "R-ALL-9731229", "reactome_id": "R-ALL-9731229", "label": "8-oxo-dATP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:172871", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9731235", "reactome_id": "R-ALL-9731235", "label": "8-oxo-dAMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:71361", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "8-oxo-(d)RTP hydrolase activity of NUDT1 [cytosol]", "activity_id": "9731237", "ec_numbers": [ "3.6.1.9" ], "accessions": [] } ], "citations": [ { "id": "PMID:10373420", "title": "The oxidized forms of dATP are substrates for the human MutT homologue, the hMTH1 protein", "journal": "J. Biol. Chem.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10373420/" }, { "id": "PMID:7782328", "title": "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria", "journal": "J. Biol. Chem.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7782328/" }, { "id": "PMID:15133035", "title": "Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15133035/" }, { "id": "PMID:10536140", "title": "Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism", "journal": "Nucleic Acids Res.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10536140/" }, { "id": "PMID:12857738", "title": "An oxidized purine nucleoside triphosphatase, MTH1, suppresses cell death caused by oxidative stress", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12857738/" } ], "x": 2381.3333333333335, "y": 3152.845238095238, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9731590", "label": "NUDT1 hydrolyzes O6-methyl-dGTP to O6-methyl-dGMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:67601" ], "source_url": "https://reactome.org/content/detail/R-HSA-9731590", "summary": "NUDT1 hydrolyzes O6-methyl-dGTP to O6-methyl-dGMP is a Reactome release 97 curated reaction event. The source record connects H2O, O(6)-methyl-dGTP(4-) to H+, O(6)-methyl-dGMP(2-), PPi in cytosol and cross-references RHEA:67601.", "equation": "H2O + O(6)-methyl-dGTP(4-) → H+ + O(6)-methyl-dGMP(2-) + PPi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731603", "reactome_id": "R-ALL-9731603", "label": "O(6)-methyl-dGTP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:169974", "formula": "C11H14N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731591", "reactome_id": "R-ALL-9731591", "label": "O(6)-methyl-dGMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:169975", "formula": "C11H14N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides of NUDT1 [cytosol]", "activity_id": "9731610", "ec_numbers": [ "3.6.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:30304478", "title": "MutT homologue 1 (MTH1) catalyzes the hydrolysis of mutagenic O6-methyl-dGTP", "journal": "Nucleic Acids Res", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30304478/" }, { "id": "PMID:7782328", "title": "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria", "journal": "J. Biol. Chem.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7782328/" }, { "id": "PMID:15133035", "title": "Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15133035/" }, { "id": "PMID:10536140", "title": "Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism", "journal": "Nucleic Acids Res.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10536140/" }, { "id": "PMID:8226881", "title": "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis", "journal": "J. Biol. Chem.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8226881/" }, { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" } ], "x": 2660, "y": 3152.845238095238, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9731613", "label": "NUDT1 hydrolyzes N6-methyl-dATP to N6-methyl-dAMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:67605" ], "source_url": "https://reactome.org/content/detail/R-HSA-9731613", "summary": "NUDT1 hydrolyzes N6-methyl-dATP to N6-methyl-dAMP is a Reactome release 97 curated reaction event. The source record connects H2O, N(6)-methyl-dATP(4-) to H+, N(6)-methyl-dAMP(2-), PPi in cytosol and cross-references RHEA:67605.", "equation": "H2O + N(6)-methyl-dATP(4-) → H+ + N(6)-methyl-dAMP(2-) + PPi", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731628", "reactome_id": "R-ALL-9731628", "label": "N(6)-methyl-dATP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:172872", "formula": "C11H14N5O12P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731609", "reactome_id": "R-ALL-9731609", "label": "N(6)-methyl-dAMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:169976", "formula": "C11H14N5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides of NUDT1 [cytosol]", "activity_id": "9731616", "ec_numbers": [ "3.6.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:7782328", "title": "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria", "journal": "J. Biol. Chem.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7782328/" }, { "id": "PMID:15133035", "title": "Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15133035/" }, { "id": "PMID:10536140", "title": "Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism", "journal": "Nucleic Acids Res.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10536140/" }, { "id": "PMID:8226881", "title": "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis", "journal": "J. Biol. Chem.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8226881/" }, { "id": "PMID:32144205", "title": "MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool", "journal": "J Biol Chem", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32144205/" }, { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" } ], "x": 2938.666666666667, "y": 3152.845238095238, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9731632", "label": "NUDT1 hydrolyzes N6-methyl-ATP to N6-methyl-AMP", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-catabolism", "purine-catabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:67609" ], "source_url": "https://reactome.org/content/detail/R-HSA-9731632", "summary": "NUDT1 hydrolyzes N6-methyl-ATP to N6-methyl-AMP is a Reactome release 97 curated reaction event. The source record connects N(6)-methyl-ATP(4-), H2O to H+, N(6)-methyl-AMP(2-), PPi in cytosol and cross-references RHEA:67609.", "equation": "N(6)-methyl-ATP(4-) + H2O → H+ + N(6)-methyl-AMP(2-) + PPi", "inputs": [ { "node_id": "R-ALL-9731617", "reactome_id": "R-ALL-9731617", "label": "N(6)-methyl-ATP(4-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:172873", "formula": "C11H14N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731626", "reactome_id": "R-ALL-9731626", "label": "N(6)-methyl-AMP(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:144842", "formula": "C11H14N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides of NUDT1 [cytosol]", "activity_id": "9731634", "ec_numbers": [ "3.6.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:7782328", "title": "Intracellular localization of 8-oxo-dGTPase in human cells, with special reference to the role of the enzyme in mitochondria", "journal": "J. Biol. Chem.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7782328/" }, { "id": "PMID:15133035", "title": "Structure of human MTH1, a Nudix family hydrolase that selectively degrades oxidized purine nucleoside triphosphates", "journal": "J. Biol. Chem.", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15133035/" }, { "id": "PMID:10536140", "title": "Multi-forms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism", "journal": "Nucleic Acids Res.", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10536140/" }, { "id": "PMID:8226881", "title": "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP, a mutagenic substrate for DNA synthesis", "journal": "J. Biol. Chem.", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8226881/" }, { "id": "PMID:32144205", "title": "MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool", "journal": "J Biol Chem", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32144205/" }, { "id": "PMID:22556419", "title": "Human MTH3 (NUDT18) Protein Hydrolyzes Oxidized Forms of Guanosine and Deoxyguanosine Diphosphates: COMPARISON WITH MTH1 AND MTH2", "journal": "J. Biol. Chem.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22556419/" } ], "x": 2381.3333333333335, "y": 3159.964285714286, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9731661", "label": "MAPDA hydrolyzes N6-methyl-dAMP to dIMP and methylamine", "schema_class": "Reaction", "domain_id": "nucleotides", "pathway_ids": [ "nucleotide-salvage" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9731661", "summary": "MAPDA hydrolyzes N6-methyl-dAMP to dIMP and methylamine is a Reactome release 97 curated reaction event. The source record connects H+, H2O, N(6)-methyl-dAMP(2-) to dIMP, methylamine in cytosol.", "equation": "H+ + H2O + N(6)-methyl-dAMP(2-) → dIMP + methylamine", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9731609", "reactome_id": "R-ALL-9731609", "label": "N(6)-methyl-dAMP(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:169976", "formula": "C11H14N5O6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9731659", "reactome_id": "R-ALL-9731659", "label": "dIMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61194", "formula": "C10H11N4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9731652", "reactome_id": "R-ALL-9731652", "label": "methylamine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:59338", "formula": "CH6N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "N6-methyl-AMP deaminase activity of MAPDA:Zn [cytosol]", "activity_id": "2161186", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:21755941", "title": "Adenosine deaminase-like protein 1 (ADAL1): characterization and substrate specificity in the hydrolysis of N(6)- or O(6)-substituted purine or 2-aminopurine nucleoside monophosphates", "journal": "J Med Chem", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21755941/" }, { "id": "PMID:32144205", "title": "MutT homologue 1 (MTH1) removes N6-methyl-dATP from the dNTP pool", "journal": "J Biol Chem", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32144205/" } ], "x": 3878.666666666667, "y": 2941.8214285714284, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9733545", "label": "Bile salts and acids bind ALB", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9733545", "summary": "Bile salts and acids bind ALB is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects ALB, bile salts and acids to ALB:bile salts and acids in extracellular region.", "equation": "ALB + bile salts and acids → ALB:bile salts and acids", "inputs": [ { "node_id": "R-HSA-194115", "reactome_id": "R-HSA-194115", "label": "ALB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-192483", "reactome_id": "R-ALL-192483", "label": "bile salts and acids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-194110", "reactome_id": "R-HSA-194110", "label": "ALB:bile salts and acids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:13416382", "title": "Bile acid content of human serum. II. The binding of cholanic acids by human plasma proteins", "journal": "J Clin Invest", "year": 1957, "url": "https://pubmed.ncbi.nlm.nih.gov/13416382/" }, { "id": "PMID:7077161", "title": "Quantitative aspects of the interaction of bile acids with human serum albumin", "journal": "J Lipid Res", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7077161/" } ], "x": 4730, "y": 1913.159090909091, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9733960", "label": "Bile salts and acids dissociate from ALB", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9733960", "summary": "Bile salts and acids dissociate from ALB is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects ALB:bile salts and acids to ALB, bile salts and acids in extracellular region.", "equation": "ALB:bile salts and acids → ALB + bile salts and acids", "inputs": [ { "node_id": "R-HSA-194110", "reactome_id": "R-HSA-194110", "label": "ALB:bile salts and acids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-194115", "reactome_id": "R-HSA-194115", "label": "ALB", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-192483", "reactome_id": "R-ALL-192483", "label": "bile salts and acids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:13416382", "title": "Bile acid content of human serum. II. The binding of cholanic acids by human plasma proteins", "journal": "J Clin Invest", "year": 1957, "url": "https://pubmed.ncbi.nlm.nih.gov/13416382/" }, { "id": "PMID:7077161", "title": "Quantitative aspects of the interaction of bile acids with human serum albumin", "journal": "J Lipid Res", "year": 1982, "url": "https://pubmed.ncbi.nlm.nih.gov/7077161/" } ], "x": 5008.666666666667, "y": 1913.159090909091, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9733964", "label": "SLC51A:SLC51B transports bile salts from cytosol to extracellular region", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol · plasma membrane · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:71855", "RHEA:72683", "RHEA:75051", "RHEA:71859", "RHEA:71703", "RHEA:71851" ], "source_url": "https://reactome.org/content/detail/R-HSA-9733964", "summary": "SLC51A:SLC51B transports bile salts from cytosol to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects bile salts and acids to bile salts and acids in cytosol · plasma membrane · extracellular region and cross-references RHEA:71855, RHEA:72683, RHEA:75051, RHEA:71859, RHEA:71703, RHEA:71851.", "equation": "bile salts and acids → bile salts and acids", "inputs": [ { "node_id": "R-ALL-194156", "reactome_id": "R-ALL-194156", "label": "bile salts and acids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-192483", "reactome_id": "R-ALL-192483", "label": "bile salts and acids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "bile acid transmembrane transporter activity of SLC51A:SLC51B [plasma membrane]", "activity_id": "9733966", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:16317684", "title": "OSTalpha-OSTbeta: a major basolateral bile acid and steroid transporter in human intestinal, renal, and biliary epithelia", "journal": "Hepatology", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16317684/" }, { "id": "PMID:17650074", "title": "Heterodimerization, trafficking and membrane topology of the two proteins, Ost alpha and Ost beta, that constitute the organic solute and steroid transporter", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17650074/" } ], "x": 4730, "y": 1535.9469696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9733969", "label": "Bile salts dissociate from FABP6", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9733969", "summary": "Bile salts dissociate from FABP6 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects bile salts and acids:FABP6 to bile salts and acids, FABP6 in cytosol.", "equation": "bile salts and acids:FABP6 → bile salts and acids + FABP6", "inputs": [ { "node_id": "R-HSA-194191", "reactome_id": "R-HSA-194191", "label": "bile salts and acids:FABP6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-194156", "reactome_id": "R-ALL-194156", "label": "bile salts and acids", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-194158", "reactome_id": "R-HSA-194158", "label": "FABP6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12486725", "title": "Insights into the bile acid transportation system: the human ileal lipid-binding protein-cholyltaurine complex and its comparison with homologous structures", "journal": "Proteins", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12486725/" }, { "id": "PMID:7588781", "title": "Molecular cloning, expression, and characterization of a human intestinal 15-kDa protein", "journal": "Eur J Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7588781/" } ], "x": 4451.333333333333, "y": 1919.0530303030303, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9733973", "label": "Bile salts bind FABP6", "schema_class": "BlackBoxEvent", "domain_id": "lipid-mediators", "pathway_ids": [ "bile-acids", "steroids" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9733973", "summary": "Bile salts bind FABP6 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects bile salts and acids, FABP6 to bile salts and acids:FABP6 in cytosol.", "equation": "bile salts and acids + FABP6 → bile salts and acids:FABP6", "inputs": [ { "node_id": "R-ALL-194156", "reactome_id": "R-ALL-194156", "label": "bile salts and acids", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-194158", "reactome_id": "R-HSA-194158", "label": "FABP6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-194191", "reactome_id": "R-HSA-194191", "label": "bile salts and acids:FABP6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:7588781", "title": "Molecular cloning, expression, and characterization of a human intestinal 15-kDa protein", "journal": "Eur J Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7588781/" }, { "id": "PMID:12486725", "title": "Insights into the bile acid transportation system: the human ileal lipid-binding protein-cholyltaurine complex and its comparison with homologous structures", "journal": "Proteins", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12486725/" } ], "x": 4730, "y": 1919.0530303030303, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9734535", "label": "SLC27A2 ligates CoA to bempedoic acid to form ETC-1002-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9734535", "summary": "SLC27A2 ligates CoA to bempedoic acid to form ETC-1002-CoA is a Reactome release 97 curated reaction event. The source record connects bempedoic acid, CoA-SH, ATP to ETC-1002-CoA, AMP, PPi in cytosol.", "equation": "bempedoic acid + CoA-SH + ATP → ETC-1002-CoA + AMP + PPi", "inputs": [ { "node_id": "R-ALL-9734538", "reactome_id": "R-ALL-9734538", "label": "bempedoic acid", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9734541", "reactome_id": "R-ALL-9734541", "label": "ETC-1002-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false }, { "node_id": "R-ALL-76577", "reactome_id": "R-ALL-76577", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-111294", "reactome_id": "R-ALL-111294", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "long-chain fatty acid-CoA ligase activity of SLC27A2 [endoplasmic reticulum membrane]", "activity_id": "193506", "ec_numbers": [ "6.2.1.3" ], "accessions": [ "O14975" ] } ], "citations": [ { "id": "PMID:27892461", "title": "Liver-specific ATP-citrate lyase inhibition by bempedoic acid decreases LDL-C and attenuates atherosclerosis", "journal": "Nat Commun", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27892461/" } ], "x": 311.33333333333337, "y": 1718.578947368421, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9734540", "label": "ACLY tetramer binds ETC-1002-CoA", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9734540", "summary": "ACLY tetramer binds ETC-1002-CoA is a Reactome release 97 curated reaction event. The source record connects ACLY tetramer, ETC-1002-CoA to ACLY tetramer:ETC-1002-CoA in cytosol.", "equation": "ACLY tetramer + ETC-1002-CoA → ACLY tetramer:ETC-1002-CoA", "inputs": [ { "node_id": "R-HSA-76188", "reactome_id": "R-HSA-76188", "label": "ACLY tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9734541", "reactome_id": "R-ALL-9734541", "label": "ETC-1002-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "ChemicalDrug", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9734534", "reactome_id": "R-HSA-9734534", "label": "ACLY tetramer:ETC-1002-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:23118444", "title": "AMP-activated protein kinase and ATP-citrate lyase are two distinct molecular targets for ETC-1002, a novel small molecule regulator of lipid and carbohydrate metabolism", "journal": "J Lipid Res", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23118444/" }, { "id": "PMID:27892461", "title": "Liver-specific ATP-citrate lyase inhibition by bempedoic acid decreases LDL-C and attenuates atherosclerosis", "journal": "Nat Commun", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27892461/" } ], "x": 311.33333333333337, "y": 1754.7894736842104, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9755937", "label": "DHCR24 reduces LAN to 24,25-dhLAN", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:33919" ], "source_url": "https://reactome.org/content/detail/R-HSA-9755937", "summary": "DHCR24 reduces LAN to 24,25-dhLAN is a Reactome release 97 curated reaction event. The source record connects LAN, NADPH, H+ to NADP+, 24,25-dhLAN in endoplasmic reticulum membrane · cytosol and cross-references RHEA:33919.", "equation": "LAN + NADPH + H+ → NADP+ + 24,25-dhLAN", "inputs": [ { "node_id": "R-ALL-194634", "reactome_id": "R-ALL-194634", "label": "LAN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16521", "formula": "C30H50O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-1130708", "reactome_id": "R-ALL-1130708", "label": "24,25-dhLAN", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28113", "formula": "C30H52O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "Delta24-sterol reductase activity of DHCR24 [endoplasmic reticulum membrane]", "activity_id": "196404", "ec_numbers": [ "1.3.1.72" ], "accessions": [ "Q15392" ] } ], "citations": [ { "id": "PMID:14404284", "title": "Preputial gland tumor sterols. 3. A metabolic pathway from lanosterol to cholesterol", "journal": "J. Biol. Chem.", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/14404284/" }, { "id": "PMID:23050906", "title": "Promoter analysis of the DHCR24 (3β-hydroxysterol Δ(24)-reductase) gene: characterization of SREBP (sterol-regulatory-element-binding protein)-mediated activation", "journal": "Biosci Rep", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23050906/" } ], "x": 2938.666666666667, "y": 2128.7, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9759202", "label": "LRP2-mediated TCN2:RCbl uptake and delivery to lysosome", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "lysosomal lumen · plasma membrane", "compartment_ids": [ "GO:0043202", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9759202", "summary": "LRP2-mediated TCN2:RCbl uptake and delivery to lysosome is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects LRP2:LDLRAP1:TCN2:RCbl to TCN2:RCbl, LRP2:LDLRAP1 in lysosomal lumen · plasma membrane.", "equation": "LRP2:LDLRAP1:TCN2:RCbl → TCN2:RCbl + LRP2:LDLRAP1", "inputs": [ { "node_id": "R-HSA-9759208", "reactome_id": "R-HSA-9759208", "label": "LRP2:LDLRAP1:TCN2:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-3000242", "reactome_id": "R-HSA-3000242", "label": "TCN2:RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9759635", "reactome_id": "R-HSA-9759635", "label": "LRP2:LDLRAP1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of LRP2:LDLRAP1:TCN2:RCbl [plasma membrane]", "activity_id": "9759383", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8706697", "title": "Cloning and sequencing of human gp330, a Ca(2+)-binding receptor with potential intracellular signaling properties", "journal": "Eur J Biochem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8706697/" }, { "id": "PMID:7768901", "title": "Identification of glycoprotein 330 as an endocytic receptor for apolipoprotein J/clusterin", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7768901/" }, { "id": "PMID:8710919", "title": "Megalin-mediated endocytosis of transcobalamin-vitamin-B12 complexes suggests a role of the receptor in vitamin-B12 homeostasis", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8710919/" }, { "id": "PMID:14528014", "title": "The adaptor protein ARH escorts megalin to and through endosomes", "journal": "Mol Biol Cell", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14528014/" }, { "id": "PMID:23836931", "title": "ARH directs megalin to the endocytic recycling compartment to regulate its proteolysis and gene expression", "journal": "J Cell Biol", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23836931/" } ], "x": 5391.333333333333, "y": 3186.8815789473683, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9759206", "label": "ABCD4:LMBRD1 transports RCbl from lysosomal lumen to cytosol", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · lysosomal lumen · lysosomal membrane", "compartment_ids": [ "GO:0005829", "GO:0043202", "GO:0005765" ], "rhea_ids": [ "RHEA:17873" ], "source_url": "https://reactome.org/content/detail/R-HSA-9759206", "summary": "ABCD4:LMBRD1 transports RCbl from lysosomal lumen to cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects ATP, H2O, RCbl to ADP, Pi, RCbl in cytosol · lysosomal lumen · lysosomal membrane and cross-references RHEA:17873.", "equation": "ATP + H2O + RCbl → ADP + Pi + RCbl", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9759390", "reactome_id": "R-ALL-9759390", "label": "RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9759394", "reactome_id": "R-ALL-9759394", "label": "RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:140785", "formula": "C62H88CoN13O14PR", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ABC-type vitamin B12 transporter activity of ABCD4:LMBRD1 [lysosomal membrane]", "activity_id": "5223338", "ec_numbers": [ "7.6.2.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:22922874", "title": "Mutations in ABCD4 cause a new inborn error of vitamin B12 metabolism", "journal": "Nat. Genet.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22922874/" }, { "id": "PMID:27456980", "title": "Translocation of the ABC transporter ABCD4 from the endoplasmic reticulum to lysosomes requires the escort protein LMBD1", "journal": "Sci Rep", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27456980/" }, { "id": "PMID:33845046", "title": "The lysosomal protein ABCD4 can transport vitamin B 12 across liposomal membranes in vitro", "journal": "J Biol Chem", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33845046/" }, { "id": "PMID:19136951", "title": "Identification of a putative lysosomal cobalamin exporter altered in the cblF defect of vitamin B12 metabolism", "journal": "Nat. Genet.", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19136951/" }, { "id": "PMID:4001945", "title": "Defect in vitamin B12 release from lysosomes: newly described inborn error of vitamin B12 metabolism", "journal": "Science", "year": 1985, "url": "https://pubmed.ncbi.nlm.nih.gov/4001945/" } ], "x": 5948.666666666667, "y": 2900.25, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9759209", "label": "LRP2 binds extracellular TCN2:RCbl", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9759209", "summary": "LRP2 binds extracellular TCN2:RCbl is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects TCN2:RCbl, LRP2:LDLRAP1 to LRP2:LDLRAP1:TCN2:RCbl in plasma membrane.", "equation": "TCN2:RCbl + LRP2:LDLRAP1 → LRP2:LDLRAP1:TCN2:RCbl", "inputs": [ { "node_id": "R-HSA-9759429", "reactome_id": "R-HSA-9759429", "label": "TCN2:RCbl", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9759635", "reactome_id": "R-HSA-9759635", "label": "LRP2:LDLRAP1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9759208", "reactome_id": "R-HSA-9759208", "label": "LRP2:LDLRAP1:TCN2:RCbl", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cargo receptor activity of LRP2:LDLRAP1 [plasma membrane]", "activity_id": "350169", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8706697", "title": "Cloning and sequencing of human gp330, a Ca(2+)-binding receptor with potential intracellular signaling properties", "journal": "Eur J Biochem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8706697/" }, { "id": "PMID:7768901", "title": "Identification of glycoprotein 330 as an endocytic receptor for apolipoprotein J/clusterin", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7768901/" }, { "id": "PMID:8710919", "title": "Megalin-mediated endocytosis of transcobalamin-vitamin-B12 complexes suggests a role of the receptor in vitamin-B12 homeostasis", "journal": "Proc Natl Acad Sci U S A", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8710919/" }, { "id": "PMID:14528014", "title": "The adaptor protein ARH escorts megalin to and through endosomes", "journal": "Mol Biol Cell", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14528014/" }, { "id": "PMID:23836931", "title": "ARH directs megalin to the endocytic recycling compartment to regulate its proteolysis and gene expression", "journal": "J Cell Biol", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23836931/" } ], "x": 5670, "y": 3145.934210526316, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9759549", "label": "Cob(I)alamin bound to MMACHC is oxidized to cob(II)alamin", "schema_class": "BlackBoxEvent", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17482" ], "source_url": "https://reactome.org/content/detail/R-HSA-9759549", "summary": "Cob(I)alamin bound to MMACHC is oxidized to cob(II)alamin is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MMACHC:cob(I)alamin, H+, NAD+ to MMACHC:cob(II)alamin, NADH in cytosol and cross-references RHEA:17482.", "equation": "2 MMACHC:cob(I)alamin + H+ + NAD+ → 2 MMACHC:cob(II)alamin + NADH", "inputs": [ { "node_id": "R-HSA-9759561", "reactome_id": "R-HSA-9759561", "label": "MMACHC:cob(I)alamin", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-3095903", "reactome_id": "R-HSA-3095903", "label": "MMACHC:cob(II)alamin", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "oxidoreductase activity of Protein [cytosol]", "activity_id": "9908094", "ec_numbers": [ "1" ], "accessions": [] } ], "citations": [ { "id": "PMID:33797888", "title": "Redox-Linked Coordination Chemistry Directs Vitamin B 12 Trafficking", "journal": "Acc Chem Res", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33797888/" }, { "id": "PMID:4390543", "title": "Enzymatic conversion of vitamin B 12a to adenosyl-B 12: evidence for the existence of two separate reducing systems", "journal": "Arch Biochem Biophys", "year": 1969, "url": "https://pubmed.ncbi.nlm.nih.gov/4390543/" } ], "x": 5391.333333333333, "y": 3043.565789473684, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-977301", "label": "Histidine is decarboxylated to histamine", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20841" ], "source_url": "https://reactome.org/content/detail/R-HSA-977301", "summary": "Histidine is decarboxylated to histamine is a Reactome release 97 curated reaction event. The source record connects L-His, H+ to Hist, CO2 in cytosol and cross-references RHEA:20841.", "equation": "L-His + H+ → Hist + CO2", "inputs": [ { "node_id": "R-ALL-29612", "reactome_id": "R-ALL-29612", "label": "L-His", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57595", "formula": "C6H9N3O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-175992", "reactome_id": "R-ALL-175992", "label": "Hist", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58432", "formula": "C5H10N3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "histidine decarboxylase activity of PXLP-K305-HDC dimer [cytosol]", "activity_id": "977296", "ec_numbers": [ "4.1.1.22" ], "accessions": [] } ], "citations": [ { "id": "PMID:1425659", "title": "Functional analysis of alternatively spliced transcripts of the human histidine decarboxylase gene and its expression in human tissues and basophilic leukemia cells", "journal": "Eur J Biochem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1425659/" } ], "x": 7740, "y": 2068.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-977317", "label": "malonyl-CoA is decarboxylated to acetyl-CoA in peroxisome", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:18782" ], "source_url": "https://reactome.org/content/detail/R-HSA-977317", "summary": "malonyl-CoA is decarboxylated to acetyl-CoA in peroxisome is a Reactome release 97 curated reaction event. The source record connects Malonyl-CoA, H+ to Ac-CoA, CO2 in peroxisomal matrix and cross-references RHEA:18782.", "equation": "Malonyl-CoA + H+ → Ac-CoA + CO2", "inputs": [ { "node_id": "R-ALL-977323", "reactome_id": "R-ALL-977323", "label": "Malonyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57384", "formula": "C24H33N7O19P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-193465", "reactome_id": "R-ALL-193465", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-353123", "reactome_id": "R-ALL-353123", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-389536", "reactome_id": "R-ALL-389536", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "malonyl-CoA decarboxylase activity of MLYCD(40-493) [peroxisomal matrix]", "activity_id": "977321", "ec_numbers": [ "4.1.1.9" ], "accessions": [ "O95822" ] } ], "citations": [ { "id": "PMID:10455107", "title": "MCD encodes peroxisomal and cytoplasmic forms of malonyl-CoA decarboxylase and is mutated in malonyl-CoA decarboxylase deficiency", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10455107/" } ], "x": 590, "y": 1594.859649122807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-977324", "label": "PSPH:Mg2+ dimer dephosphorylates O-P-Ser", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "serine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21209" ], "source_url": "https://reactome.org/content/detail/R-HSA-977324", "summary": "PSPH:Mg2+ dimer dephosphorylates O-P-Ser is a Reactome release 97 curated reaction event. The source record connects O-P-Ser, H2O to L-Ser, Pi in cytosol and cross-references RHEA:21209.", "equation": "O-P-Ser + H2O → L-Ser + Pi", "inputs": [ { "node_id": "R-ALL-936706", "reactome_id": "R-ALL-936706", "label": "O-P-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57524", "formula": "C3H6NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-phosphoserine phosphatase activity of PSPH:Mg2+ dimer [cytosol]", "activity_id": "977341", "ec_numbers": [ "3.1.3.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:9188776", "title": "Human L-3-phosphoserine phosphatase: sequence, expression and evidence for a phosphoenzyme intermediate", "journal": "FEBS Lett", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9188776/" } ], "x": 590, "y": 2841.222222222222, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-977333", "label": "PXLP-K200-PSAT1 dimer transfers amino group from L-Glu to 3POPA", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "serine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:14331" ], "source_url": "https://reactome.org/content/detail/R-HSA-977333", "summary": "PXLP-K200-PSAT1 dimer transfers amino group from L-Glu to 3POPA is a Reactome release 97 curated reaction event. The source record connects 3POPA, L-Glu to 2OG, O-P-Ser in cytosol and cross-references RHEA:14331.", "equation": "3POPA + L-Glu → 2OG + O-P-Ser", "inputs": [ { "node_id": "R-ALL-977329", "reactome_id": "R-ALL-977329", "label": "3POPA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18110", "formula": "C3H2O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29404", "reactome_id": "R-ALL-29404", "label": "L-Glu", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:29985", "formula": "C5H8NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29406", "reactome_id": "R-ALL-29406", "label": "2OG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16810", "formula": "C5H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-936706", "reactome_id": "R-ALL-936706", "label": "O-P-Ser", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57524", "formula": "C3H6NO6P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "O-phospho-L-serine:2-oxoglutarate transaminase activity of PXLP-K200-PSAT1 dimer [cytosol]", "activity_id": "977342", "ec_numbers": [ "2.6.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:12633500", "title": "Characterization of human phosphoserine aminotransferase involved in the phosphorylated pathway of L-serine biosynthesis", "journal": "Biochem J", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12633500/" } ], "x": 868.6666666666667, "y": 2841.222222222222, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-977348", "label": "PHGDH tetramer dehydrogenates 3PG", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "serine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:12642" ], "source_url": "https://reactome.org/content/detail/R-HSA-977348", "summary": "PHGDH tetramer dehydrogenates 3PG is a Reactome release 97 curated reaction event. The source record connects 3PG, NAD+ to 3POPA, NADH, H+ in cytosol and cross-references RHEA:12642.", "equation": "3PG + NAD+ → 3POPA + NADH + H+", "inputs": [ { "node_id": "R-ALL-29728", "reactome_id": "R-ALL-29728", "label": "3PG", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58272", "formula": "C3H4O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-977329", "reactome_id": "R-ALL-977329", "label": "3POPA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18110", "formula": "C3H2O7P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphoglycerate dehydrogenase activity of PHGDH tetramer [cytosol]", "activity_id": "977334", "ec_numbers": [ "1.1.1.95" ], "accessions": [] } ], "citations": [ { "id": "PMID:19235232", "title": "Novel mutations in 3-phosphoglycerate dehydrogenase (PHGDH) are distributed throughout the protein and result in altered enzyme kinetics", "journal": "Hum Mutat", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19235232/" } ], "x": 311.33333333333337, "y": 2841.222222222222, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9773976", "label": "AKR1E2 reduces 1,5-aF to 1,5-aG", "schema_class": "Reaction", "domain_id": "carbohydrate-core", "pathway_ids": [ "glycogen" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20667" ], "source_url": "https://reactome.org/content/detail/R-HSA-9773976", "summary": "AKR1E2 reduces 1,5-aF to 1,5-aG is a Reactome release 97 curated reaction event. The source record connects 1,5-aF, H+, NADPH to 1,5-aG, NADP+ in cytosol and cross-references RHEA:20667.", "equation": "1,5-aF + H+ + NADPH → 1,5-aG + NADP+", "inputs": [ { "node_id": "R-ALL-9773955", "reactome_id": "R-ALL-9773955", "label": "1,5-aF", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16715", "formula": "C6H10O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9773958", "reactome_id": "R-ALL-9773958", "label": "1,5-aG", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16070", "formula": "C6H12O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "1,5-anhydro-D-fructose reductase activity of AKR1E2 [cytosol]", "activity_id": "9773965", "ec_numbers": [ "1.1.1.263" ], "accessions": [ "Q96JD6" ] } ], "citations": [ { "id": "PMID:15118078", "title": "Characterization of htAKR, a novel gene product in the aldo-keto reductase family specifically expressed in human testis", "journal": "Mol Hum Reprod", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15118078/" }, { "id": "PMID:9022716", "title": "Hepatic production of 1,5-anhydrofructose and 1,5-anhydroglucitol in rat by the third glycogenolytic pathway", "journal": "Eur J Biochem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/9022716/" }, { "id": "PMID:12604216", "title": "Human testis specific protein: a new member of aldo-keto reductase superfamily", "journal": "Chem Biol Interact", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12604216/" }, { "id": "PMID:18323634", "title": "Mouse AKR1E1 is an ortholog of pig liver NADPH dependent 1,5-anhydro-D-fructose reductase", "journal": "Biosci Biotechnol Biochem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18323634/" }, { "id": "PMID:9504428", "title": "Purification and some properties of a hepatic NADPH-dependent reductase that specifically acts on 1,5-anhydro-D-fructose", "journal": "J Biochem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9504428/" }, { "id": "PMID:35534727", "title": "A glucose-like metabolite deficient in diabetes inhibits cellular entry of SARS-CoV-2", "journal": "Nat Metab", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35534727/" } ], "x": 1530, "y": 756.9615384615385, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9792568", "label": "CTSL1 cleaves proHPSE", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9792568", "summary": "CTSL1 cleaves proHPSE is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects proHPSE to HPSE dimer in lysosomal lumen.", "equation": "proHPSE → HPSE dimer", "inputs": [ { "node_id": "R-HSA-9792563", "reactome_id": "R-HSA-9792563", "label": "proHPSE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1666976", "reactome_id": "R-HSA-1666976", "label": "HPSE dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "cysteine-type endopeptidase activity of CTSL1(114-288) [lysosomal lumen]", "activity_id": "9792571", "ec_numbers": [ "3.4.22.1" ], "accessions": [ "P07711" ] } ], "citations": [ { "id": "PMID:18450756", "title": "Cathepsin L is responsible for processing and activation of proheparanase through multiple cleavages of a linker segment", "journal": "J Biol Chem", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18450756/" } ], "x": 2938.666666666667, "y": 1077.9545454545455, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9792569", "label": "proHPSE binds SDC1", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9792569", "summary": "proHPSE binds SDC1 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects HS(6)-SDC1, proHPSE to proHPSE:SDC1 in plasma membrane.", "equation": "HS(6)-SDC1 + proHPSE → proHPSE:SDC1", "inputs": [ { "node_id": "R-HSA-2076652", "reactome_id": "R-HSA-2076652", "label": "HS(6)-SDC1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9792564", "reactome_id": "R-HSA-9792564", "label": "proHPSE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9792578", "reactome_id": "R-HSA-9792578", "label": "proHPSE:SDC1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:20576607", "title": "Heparanase 2 interacts with heparan sulfate with high affinity and inhibits heparanase activity", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20576607/" }, { "id": "PMID:24788042", "title": "Processing of heparanase is mediated by syndecan-1 cytoplasmic domain and involves syntenin and α-actinin", "journal": "Cell Mol Life Sci", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24788042/" } ], "x": 2660, "y": 1054.378787878788, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9792581", "label": "proHPSE:SDC1 is delivered to lysosomes", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen · plasma membrane", "compartment_ids": [ "GO:0043202", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9792581", "summary": "proHPSE:SDC1 is delivered to lysosomes is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects proHPSE:SDC1 to proHPSE:SDC1 in lysosomal lumen · plasma membrane.", "equation": "proHPSE:SDC1 → proHPSE:SDC1", "inputs": [ { "node_id": "R-HSA-9792578", "reactome_id": "R-HSA-9792578", "label": "proHPSE:SDC1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9792577", "reactome_id": "R-HSA-9792577", "label": "proHPSE:SDC1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24788042", "title": "Processing of heparanase is mediated by syndecan-1 cytoplasmic domain and involves syntenin and α-actinin", "journal": "Cell Mol Life Sci", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24788042/" } ], "x": 2381.3333333333335, "y": 1125.1060606060605, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9837333", "label": "SLC25A42 exchanges cytosolic CoA-SH for mitochondrial matrix ADP", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [ "RHEA:72839" ], "source_url": "https://reactome.org/content/detail/R-HSA-9837333", "summary": "SLC25A42 exchanges cytosolic CoA-SH for mitochondrial matrix ADP is a Reactome release 97 curated reaction event. The source record connects ADP, CoA-SH to ADP, CoA-SH in cytosol · mitochondrial matrix and cross-references RHEA:72839.", "equation": "ADP + CoA-SH → ADP + CoA-SH", "inputs": [ { "node_id": "R-ALL-113581", "reactome_id": "R-ALL-113581", "label": "ADP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "coenzyme A transmembrane transporter activity of SLC25A42 [mitochondrial inner membrane]", "activity_id": "9837330", "ec_numbers": null, "accessions": [ "Q86VD7" ] } ], "citations": [ { "id": "PMID:36983025", "title": "Inherited Disorders of Coenzyme A Biosynthesis: Models, Mechanisms, and Treatments", "journal": "Int J Mol Sci", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36983025/" }, { "id": "PMID:19429682", "title": "A novel member of solute carrier family 25 (SLC25A42) is a transporter of coenzyme A and adenosine 3',5'-diphosphate in human mitochondria", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19429682/" } ], "x": 5948.666666666667, "y": 2992.3815789473683, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9837337", "label": "DCAKD phosphorylates DP-CoA", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:18246" ], "source_url": "https://reactome.org/content/detail/R-HSA-9837337", "summary": "DCAKD phosphorylates DP-CoA is a Reactome release 97 curated reaction event. The source record connects ATP, DP-CoA to ADP, CoA-SH, H+ in cytosol · mitochondrial outer membrane and cross-references RHEA:18246.", "equation": "ATP + DP-CoA → ADP + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-196778", "reactome_id": "R-ALL-196778", "label": "DP-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57328", "formula": "C21H33N7O13P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "dephospho-CoA kinase activity of DCAKD [mitochondrial outer membrane]", "activity_id": "9837345", "ec_numbers": [ "2.7.1.24" ], "accessions": [ "Q8WVC6" ] } ], "citations": [ { "id": "PMID:11923312", "title": "Complete reconstitution of the human coenzyme A biosynthetic pathway via comparative genomics", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11923312/" } ], "x": 5948.666666666667, "y": 2941.1973684210525, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9837354", "label": "NUDT8 hydrolyzes CoA-SH to PPANT", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:64989" ], "source_url": "https://reactome.org/content/detail/R-HSA-9837354", "summary": "NUDT8 hydrolyzes CoA-SH to PPANT is a Reactome release 97 curated reaction event. The source record connects CoA-SH, H2O to H+, PAP, PPANT in mitochondrial matrix and cross-references RHEA:64989.", "equation": "CoA-SH + H2O → 2 H+ + PAP + PPANT", "inputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9837341", "reactome_id": "R-ALL-9837341", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9837343", "reactome_id": "R-ALL-9837343", "label": "PPANT", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:61723", "formula": "C11H21N2O7PS", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "coenzyme A diphosphatase activity of NUDT8 [mitochondrial matrix]", "activity_id": "9837350", "ec_numbers": [ "3.6.1.77" ], "accessions": [ "Q8WV74" ] } ], "citations": [ { "id": "PMID:31004344", "title": "Nudt8 is a novel CoA diphosphohydrolase that resides in the mitochondria", "journal": "FEBS Lett", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31004344/" } ], "x": 5391.333333333333, "y": 2951.434210526316, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9837419", "label": "PANK4 hydrolyzes PPANT to pantetheine", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:68329" ], "source_url": "https://reactome.org/content/detail/R-HSA-9837419", "summary": "PANK4 hydrolyzes PPANT to pantetheine is a Reactome release 97 curated reaction event. The source record connects PPANT, H2O to Pantetheine, Pi in cytosol and cross-references RHEA:68329.", "equation": "PPANT + H2O → Pantetheine + Pi", "inputs": [ { "node_id": "R-ALL-196767", "reactome_id": "R-ALL-196767", "label": "PPANT", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:61723", "formula": "C11H21N2O7PS", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-8938313", "reactome_id": "R-ALL-8938313", "label": "Pantetheine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16753", "formula": "C11H22N2O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatase activity of PANK4 [cytosol]", "activity_id": "9837405", "ec_numbers": [ "3.1.3" ], "accessions": [ "Q9NVE7" ] } ], "citations": [ { "id": "PMID:27322068", "title": "A family of metal-dependent phosphatases implicated in metabolite damage-control", "journal": "Nat Chem Biol", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27322068/" }, { "id": "PMID:32234503", "title": "Regulation of coenzyme A levels by degradation: the 'Ins and Outs'", "journal": "Prog Lipid Res", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32234503/" } ], "x": 5391.333333333333, "y": 3002.6184210526317, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9840334", "label": "PSAP is cleaved", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen · lysosomal membrane", "compartment_ids": [ "GO:0043202", "GO:0005765" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840334", "summary": "PSAP is cleaved is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects PSAP to PSAP(195-273), PSAP(311-391), PSAP(405-486) in lysosomal lumen · lysosomal membrane.", "equation": "PSAP → PSAP(195-273) + PSAP(311-391) + PSAP(405-486) + PSAP(60-142)", "inputs": [ { "node_id": "R-HSA-9840325", "reactome_id": "R-HSA-9840325", "label": "PSAP", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9840336", "reactome_id": "R-HSA-9840336", "label": "PSAP(195-273)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-1605602", "reactome_id": "R-HSA-1605602", "label": "PSAP(311-391)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9840328", "reactome_id": "R-HSA-9840328", "label": "PSAP(405-486)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-351333", "reactome_id": "R-HSA-351333", "label": "PSAP(60-142)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16230343", "title": "Lipid-binding proteins in membrane digestion, antigen presentation, and antimicrobial defense", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16230343/" }, { "id": "PMID:29443946", "title": "A Comparative Study of Human Saposins", "journal": "Molecules", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29443946/" }, { "id": "PMID:8573994", "title": "Molecular role of sulfated glycoprotein-1 (SGP-1/prosaposin) in Sertoli cells", "journal": "Histol Histopathol", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8573994/" }, { "id": "PMID:2001789", "title": "Saposin proteins: structure, function, and role in human lysosomal storage disorders", "journal": "FASEB J", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/2001789/" }, { "id": "PMID:19836391", "title": "Lysosomal degradation of membrane lipids", "journal": "FEBS Lett", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/19836391/" }, { "id": "PMID:8663398", "title": "Proteolytic processing patterns of prosaposin in insect and mammalian cells", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8663398/" } ], "x": 3600, "y": 1914.5192307692307, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9840470", "label": "PSAP(195-273) dimer:PE binds and mobilizes ligands", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen · multivesicular body, internal vesicle membrane", "compartment_ids": [ "GO:0043202", "GO:0097488" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840470", "summary": "PSAP(195-273) dimer:PE binds and mobilizes ligands is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PSAP(195-273):PE, PSAP(195-273) ligands to PSAP(195-273):Ligands:PE in lysosomal lumen · multivesicular body, internal vesicle membrane.", "equation": "PSAP(195-273):PE + PSAP(195-273) ligands → PSAP(195-273):Ligands:PE", "inputs": [ { "node_id": "R-HSA-9842117", "reactome_id": "R-HSA-9842117", "label": "PSAP(195-273):PE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9842141", "reactome_id": "R-ALL-9842141", "label": "PSAP(195-273) ligands", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "multivesicular body, internal vesicle membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9840472", "reactome_id": "R-HSA-9840472", "label": "PSAP(195-273):Ligands:PE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "sphingolipid activator protein activity of PSAP(195-273) dimer [lysosomal lumen]", "activity_id": "9840712", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:10942779", "title": "Degradation of membrane-bound ganglioside GM1. Stimulation by bis(monoacylglycero)phosphate and the activator proteins SAP-B and GM2-AP", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10942779/" }, { "id": "PMID:12518053", "title": "Crystal structure of saposin B reveals a dimeric shell for lipid binding", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12518053/" }, { "id": "PMID:1889421", "title": "Glycosphingolipid specificity of the human sulfatide activator protein", "journal": "Eur J Biochem", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1889421/" }, { "id": "PMID:29802621", "title": "Emerging concepts of ganglioside metabolism", "journal": "FEBS Lett", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29802621/" } ], "x": 3600, "y": 1884.5961538461538, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9840488", "label": "PSAP(195-273) forms a dimer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840488", "summary": "PSAP(195-273) forms a dimer is a Reactome release 97 curated reaction event. The source record connects PSAP(195-273) to PSAP(195-273) dimer in lysosomal lumen.", "equation": "2 PSAP(195-273) → PSAP(195-273) dimer", "inputs": [ { "node_id": "R-HSA-9840336", "reactome_id": "R-HSA-9840336", "label": "PSAP(195-273)", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9840482", "reactome_id": "R-HSA-9840482", "label": "PSAP(195-273) dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:12510003", "title": "Expression, purification, crystallization, and preliminary X-ray analysis of recombinant human saposin B", "journal": "Protein Expr Purif", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12510003/" }, { "id": "PMID:7730378", "title": "Structural analysis of saposin C and B. Complete localization of disulfide bridges", "journal": "J Biol Chem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7730378/" }, { "id": "PMID:12518053", "title": "Crystal structure of saposin B reveals a dimeric shell for lipid binding", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12518053/" } ], "x": 3600, "y": 1899.5576923076924, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9840579", "label": "GM1 localizes to ILV membrane", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "endosome membrane · multivesicular body, internal vesicle membrane", "compartment_ids": [ "GO:0010008", "GO:0097488" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840579", "summary": "GM1 localizes to ILV membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects GM1-d18 to GM1 in endosome membrane · multivesicular body, internal vesicle membrane.", "equation": "GM1-d18 → GM1", "inputs": [ { "node_id": "R-ALL-9840584", "reactome_id": "R-ALL-9840584", "label": "GM1-d18", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:73110", "formula": "C73H130N3O31", "compartment": "endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9840481", "reactome_id": "R-ALL-9840481", "label": "GM1", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:82639", "formula": "C41H66N3O31R2", "compartment": "multivesicular body, internal vesicle membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:29802621", "title": "Emerging concepts of ganglioside metabolism", "journal": "FEBS Lett", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29802621/" } ], "x": 3878.666666666667, "y": 1899.5576923076924, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9840581", "label": "GM1 localizes to endosome membrane", "schema_class": "BlackBoxEvent", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "endosome membrane · plasma membrane", "compartment_ids": [ "GO:0010008", "GO:0005886" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840581", "summary": "GM1 localizes to endosome membrane is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects GM1 to GM1-d18 in endosome membrane · plasma membrane.", "equation": "GM1 → GM1-d18", "inputs": [ { "node_id": "R-ALL-9840585", "reactome_id": "R-ALL-9840585", "label": "GM1", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:82639", "formula": "C41H66N3O31R2", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9840584", "reactome_id": "R-ALL-9840584", "label": "GM1-d18", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:73110", "formula": "C73H130N3O31", "compartment": "endosome membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:29802621", "title": "Emerging concepts of ganglioside metabolism", "journal": "FEBS Lett", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29802621/" } ], "x": 3321.3333333333335, "y": 1914.5192307692307, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9840795", "label": "Beta-galactosidases hydrolyze GM2A:GA1 to GM2A:GA2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840795", "summary": "Beta-galactosidases hydrolyze GM2A:GA1 to GM2A:GA2 is a Reactome release 97 curated reaction event. The source record connects H2O, GM2A:GA1 to Gal, GM2A:GA2 in lysosomal lumen.", "equation": "H2O + GM2A:GA1 → Gal + GM2A:GA2", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9840793", "reactome_id": "R-HSA-9840793", "label": "GM2A:GA1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605637", "reactome_id": "R-ALL-1605637", "label": "Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840792", "reactome_id": "R-HSA-9840792", "label": "GM2A:GA2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "beta-galactosidase activity of BGAL [lysosomal lumen]", "activity_id": "1605789", "ec_numbers": [ "3.2.1.23" ], "accessions": [] } ], "citations": [ { "id": "PMID:15714521", "title": "Role of beta-galactosidase and elastin binding protein in lysosomal and nonlysosomal complexes of patients with GM1-gangliosidosis", "journal": "Hum Mutat", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15714521/" } ], "x": 3600, "y": 1854.673076923077, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9840833", "label": "bHEXA,bHEXB hydrolyze GM2A:GA2 to GM2A:LacCer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840833", "summary": "bHEXA,bHEXB hydrolyze GM2A:GA2 to GM2A:LacCer is a Reactome release 97 curated reaction event. The source record connects H2O, GM2A:GA2 to GalNAc, GM2A:LacCer in lysosomal lumen.", "equation": "H2O + GM2A:GA2 → GalNAc + GM2A:LacCer", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9840792", "reactome_id": "R-HSA-9840792", "label": "GM2A:GA2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605658", "reactome_id": "R-ALL-1605658", "label": "GalNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28037", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840837", "reactome_id": "R-HSA-9840837", "label": "GM2A:LacCer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXA,bHEXB [lysosomal lumen]", "activity_id": "1605761", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:5135907", "title": "Enzyme alterations and lipid storage in three variants of Tay-Sachs disease", "journal": "J Neurochem", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/5135907/" }, { "id": "PMID:29747811", "title": "Ganglioside Metabolism in Health and Disease", "journal": "Prog Mol Biol Transl Sci", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29747811/" }, { "id": "PMID:29802621", "title": "Emerging concepts of ganglioside metabolism", "journal": "FEBS Lett", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29802621/" } ], "x": 3878.666666666667, "y": 1854.673076923077, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9840884", "label": "bHEXA,bHEXS hydrolyze GM2A:SM2", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840884", "summary": "bHEXA,bHEXS hydrolyze GM2A:SM2 is a Reactome release 97 curated reaction event. The source record connects GM2A:SM2, H2O to GalNAc, SM3, GM2A(32-193) in lysosomal lumen.", "equation": "GM2A:SM2 + H2O → GalNAc + SM3 + GM2A(32-193)", "inputs": [ { "node_id": "R-HSA-9840882", "reactome_id": "R-HSA-9840882", "label": "GM2A:SM2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1605658", "reactome_id": "R-ALL-1605658", "label": "GalNAc", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28037", "formula": "C8H15NO6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9840879", "reactome_id": "R-ALL-9840879", "label": "SM3", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:90163", "formula": "C16H26NO16SR2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-1605614", "reactome_id": "R-HSA-1605614", "label": "GM2A(32-193)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "beta-N-acetylhexosaminidase activity of bHEXA,bHEXS [lysosomal lumen]", "activity_id": "9840888", "ec_numbers": [ "3.2.1.52" ], "accessions": [] } ], "citations": [ { "id": "PMID:11707436", "title": "Physiological substrates for human lysosomal beta -hexosaminidase S", "journal": "J Biol Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/11707436/" } ], "x": 3600, "y": 1794.826923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9840949", "label": "ARSA removes sulfate from SM3", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9840949", "summary": "ARSA removes sulfate from SM3 is a Reactome release 97 curated reaction event. The source record connects SM3, H2O to LacCer, SO4(2-) in lysosomal lumen.", "equation": "SM3 + H2O → LacCer + SO4(2-)", "inputs": [ { "node_id": "R-ALL-9840879", "reactome_id": "R-ALL-9840879", "label": "SM3", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:90163", "formula": "C16H26NO16SR2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1605601", "reactome_id": "R-ALL-1605601", "label": "LacCer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:195546", "formula": "C16H27NO13R2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1606845", "reactome_id": "R-ALL-1606845", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "cerebroside-sulfatase activity of active ARSA:Ca2+ [lysosomal lumen]", "activity_id": "1606828", "ec_numbers": [ "3.1.6.8" ], "accessions": [] } ], "citations": [ { "id": "PMID:11350180", "title": "Characterization of urinary sulfatides in metachromatic leukodystrophy using electrospray ionization-tandem mass spectrometry", "journal": "Mol Genet Metab", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11350180/" }, { "id": "PMID:5164093", "title": "Human urinary sulfatides in patients with sulfatidosis (metachromatic leukodystrophy)", "journal": "J Lipid Res", "year": 1971, "url": "https://pubmed.ncbi.nlm.nih.gov/5164093/" } ], "x": 3878.666666666667, "y": 1764.9038461538462, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9841189", "label": "GLA hydrolyzes PSAP(195-273):Gal2Cer:PE", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9841189", "summary": "GLA hydrolyzes PSAP(195-273):Gal2Cer:PE is a Reactome release 97 curated reaction event. The source record connects H2O, PSAP(195-273):Gal2Cer:PE to Gal, GalCer, PSAP(195-273) dimer in lysosomal lumen.", "equation": "H2O + PSAP(195-273):Gal2Cer:PE → Gal + GalCer + PSAP(195-273) dimer + PE 30,32,34", "inputs": [ { "node_id": "R-ALL-1605715", "reactome_id": "R-ALL-1605715", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9841186", "reactome_id": "R-HSA-9841186", "label": "PSAP(195-273):Gal2Cer:PE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1605637", "reactome_id": "R-ALL-1605637", "label": "Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1606573", "reactome_id": "R-ALL-1606573", "label": "GalCer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36498", "formula": "C10H17NO8R2", "compartment": "lysosomal lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9840482", "reactome_id": "R-HSA-9840482", "label": "PSAP(195-273) dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9840939", "reactome_id": "R-ALL-9840939", "label": "PE 30,32,34", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "alpha-galactosidase activity of GLA dimer [lysosomal lumen]", "activity_id": "1605696", "ec_numbers": [ "3.2.1.22" ], "accessions": [] } ], "citations": [ { "id": "PMID:3014515", "title": "Human alpha-galactosidase A: nucleotide sequence of a cDNA clone encoding the mature enzyme", "journal": "Proc Natl Acad Sci U S A", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3014515/" }, { "id": "PMID:15003450", "title": "The molecular defect leading to Fabry disease: structure of human alpha-galactosidase", "journal": "J Mol Biol", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15003450/" }, { "id": "PMID:7504405", "title": "Nature and frequency of mutations in the alpha-galactosidase A gene that cause Fabry disease", "journal": "Am J Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/7504405/" }, { "id": "PMID:16595074", "title": "Fabry disease: identification of 50 novel alpha-galactosidase A mutations causing the classic phenotype and three-dimensional structural analysis of 29 missense mutations", "journal": "Hum Genomics", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16595074/" }, { "id": "PMID:8370580", "title": "Prosaposin deficiency: further characterization of the sphingolipid activator protein-deficient sibs. Multiple glycolipid elevations (including lactosylceramidosis), partial enzyme deficiencies and ultrastructure of the skin in this generalized sphingolipid storage disease", "journal": "Hum Genet", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8370580/" }, { "id": "PMID:39940", "title": "Studies on human liver alpha-galactosidases. I. Purification of alpha-galactosidase A and its enzymatic properties with glycolipid and oligosaccharide substrates", "journal": "J Biol Chem", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/39940/" } ], "x": 3878.666666666667, "y": 1794.826923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9842115", "label": "PSAP(195-273) dimer binds PE", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "lysosomal lumen · multivesicular body, internal vesicle membrane", "compartment_ids": [ "GO:0043202", "GO:0097488" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9842115", "summary": "PSAP(195-273) dimer binds PE is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PSAP(195-273) dimer, PE 30,32,34 to PSAP(195-273):PE in lysosomal lumen · multivesicular body, internal vesicle membrane.", "equation": "PSAP(195-273) dimer + PE 30,32,34 → PSAP(195-273):PE", "inputs": [ { "node_id": "R-HSA-9840482", "reactome_id": "R-HSA-9840482", "label": "PSAP(195-273) dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9840942", "reactome_id": "R-ALL-9840942", "label": "PE 30,32,34", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "multivesicular body, internal vesicle membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9842117", "reactome_id": "R-HSA-9842117", "label": "PSAP(195-273):PE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "phosphatidylethanolamine binding activity of PSAP(195-273) dimer [lysosomal lumen]", "activity_id": "9956908", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:12518053", "title": "Crystal structure of saposin B reveals a dimeric shell for lipid binding", "journal": "Proc Natl Acad Sci U S A", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12518053/" } ], "x": 3878.666666666667, "y": 1884.5961538461538, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9843721", "label": "ABCC1,ABCG2 transport C18-S1P to extracellular region", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "cytosol · plasma membrane · extracellular region", "compartment_ids": [ "GO:0005829", "GO:0005886", "GO:0005576" ], "rhea_ids": [ "RHEA:38951" ], "source_url": "https://reactome.org/content/detail/R-HSA-9843721", "summary": "ABCC1,ABCG2 transport C18-S1P to extracellular region is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects C18-S1P, ATP, H2O to C18-S1P, ADP, H+ in cytosol · plasma membrane · extracellular region and cross-references RHEA:38951.", "equation": "C18-S1P + ATP + H2O → C18-S1P + ADP + H+ + Pi", "inputs": [ { "node_id": "R-ALL-428203", "reactome_id": "R-ALL-428203", "label": "C18-S1P", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:60119", "formula": "C18H37NO5P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-419305", "reactome_id": "R-ALL-419305", "label": "C18-S1P", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60119", "formula": "C18H37NO5P", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sphingolipid intramembrane carrier activity of ABCC1,ABCG2 [plasma membrane]", "activity_id": "9843728", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:20110355", "title": "Estradiol induces export of sphingosine 1-phosphate from breast cancer cells via ABCC1 and ABCG2", "journal": "J Biol Chem", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20110355/" }, { "id": "PMID:17050692", "title": "Role of ABCC1 in export of sphingosine-1-phosphate from mast cells", "journal": "Proc Natl Acad Sci U S A", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17050692/" }, { "id": "PMID:19370318", "title": "Involvement of the ABC-transporter ABCC1 and the sphingosine 1-phosphate receptor subtype S1P(3) in the cytoprotection of human fibroblasts by the glucocorticoid dexamethasone", "journal": "J Mol Med (Berl)", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19370318/" } ], "x": 3878.666666666667, "y": 1540.4807692307693, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9844587", "label": "GAL3ST1 transfers sulfate to GalCer,LacCer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane", "compartment_ids": [ "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9844587", "summary": "GAL3ST1 transfers sulfate to GalCer,LacCer is a Reactome release 97 curated reaction event. The source record connects PAPS, GalCer,LacCer to PAP, H+, SM3,SM4 in Golgi membrane.", "equation": "PAPS + GalCer,LacCer → PAP + H+ + SM3,SM4", "inputs": [ { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9845491", "reactome_id": "R-ALL-9845491", "label": "GalCer,LacCer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9845488", "reactome_id": "R-ALL-9845488", "label": "SM3,SM4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "galactosylceramide sulfotransferase activity of GAL3ST1 [Golgi membrane]", "activity_id": "1638107", "ec_numbers": [ "2.8.2.11" ], "accessions": [ "Q99999" ] } ], "citations": [ { "id": "PMID:9030544", "title": "Molecular cloning and expression of cDNA encoding human 3'-phosphoadenylylsulfate:galactosylceramide 3'-sulfotransferase", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9030544/" }, { "id": "PMID:8830034", "title": "Purification and characterization of 3'-phosphoadenosine-5'-phosphosulfate:GalCer sulfotransferase from human renal cancer cells", "journal": "J Biochem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8830034/" }, { "id": "PMID:23574804", "title": "Biosynthesis and biological function of sulfoglycolipids", "journal": "Proc Jpn Acad Ser B Phys Biol Sci", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23574804/" } ], "x": 3321.3333333333335, "y": 1809.7884615384614, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9844860", "label": "ST3GAL5 transfers NeuNAc to ceramides", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:41776" ], "source_url": "https://reactome.org/content/detail/R-HSA-9844860", "summary": "ST3GAL5 transfers NeuNAc to ceramides is a Reactome release 97 curated reaction event. The source record connects LacCer/GalCer/GA1/GA2, CMP-Neu5Ac to GM1/2/3/4, CMP, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:41776.", "equation": "LacCer/GalCer/GA1/GA2 + CMP-Neu5Ac → GM1/2/3/4 + CMP + H+", "inputs": [ { "node_id": "R-ALL-9844855", "reactome_id": "R-ALL-9844855", "label": "LacCer/GalCer/GA1/GA2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-727815", "reactome_id": "R-ALL-727815", "label": "CMP-Neu5Ac", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57812", "formula": "C20H29N4O16P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9844871", "reactome_id": "R-ALL-9844871", "label": "GM1/2/3/4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-727810", "reactome_id": "R-ALL-727810", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "beta-galactoside (CMP) alpha-2,3-sialyltransferase activity of ST3GAL5 [Golgi membrane]", "activity_id": "9844856", "ec_numbers": [ "2.4.3.4" ], "accessions": [ "Q9UNP4" ] } ], "citations": [ { "id": "PMID:9822625", "title": "Expression cloning and functional characterization of human cDNA for ganglioside GM3 synthase", "journal": "J. Biol. Chem.", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9822625/" }, { "id": "PMID:23290777", "title": "Ceramide glycosylation catalyzed by glucosylceramide synthase and cancer drug resistance", "journal": "Adv. Cancer Res.", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23290777/" }, { "id": "PMID:16934889", "title": "Human GM3 synthase: a new mRNA variant encodes an NH2-terminal extended form of the protein", "journal": "Biochim Biophys Acta", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16934889/" }, { "id": "PMID:11030748", "title": "Evidence supporting a late Golgi location for lactosylceramide to ganglioside GM3 conversion", "journal": "Glycobiology", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/11030748/" }, { "id": "PMID:10764824", "title": "Molecular identification, tissue distribution and subcellular localization of mST3GalV/GM3 synthase", "journal": "Glycobiology", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10764824/" }, { "id": "PMID:35628171", "title": "Ganglioside GM3 Synthase Deficiency in Mouse Models and Human Patients", "journal": "Int J Mol Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35628171/" } ], "x": 3321.3333333333335, "y": 1660.173076923077, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9845516", "label": "B3GALT4 transfers Gal to gangliosides", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9845516", "summary": "B3GALT4 transfers Gal to gangliosides is a Reactome release 97 curated reaction event. The source record connects GM2,GD2,GA2, UDP-Gal to GM1,GD1b,GA1, UDP, H+ in Golgi membrane · Golgi lumen.", "equation": "GM2,GD2,GA2 + UDP-Gal → GM1,GD1b,GA1 + UDP + H+", "inputs": [ { "node_id": "R-ALL-9845519", "reactome_id": "R-ALL-9845519", "label": "GM2,GD2,GA2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-735682", "reactome_id": "R-ALL-735682", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9845510", "reactome_id": "R-ALL-9845510", "label": "GM1,GD1b,GA1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N-acetyl-beta-D-glucosaminide beta-(1,3)-galactosyltransferase activity of B3GALT4 [Golgi membrane]", "activity_id": "9845518", "ec_numbers": [ "2.4.1.86" ], "accessions": [ "O96024" ] } ], "citations": [ { "id": "PMID:9582303", "title": "A family of human beta3-galactosyltransferases. Characterization of four members of a UDP-galactose:beta-N-acetyl-glucosamine/beta-nacetyl-galactosamine beta-1,3-galactosyltransferase family", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9582303/" }, { "id": "PMID:12222957", "title": "The galactosyltransferase family", "journal": "Cell. Mol. Life Sci.", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12222957/" } ], "x": 3600, "y": 1809.7884615384614, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9845538", "label": "ST3GAL2,3 transfer Neu5Ac to gangliosides", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:47572" ], "source_url": "https://reactome.org/content/detail/R-HSA-9845538", "summary": "ST3GAL2,3 transfer Neu5Ac to gangliosides is a Reactome release 97 curated reaction event. The source record connects CMP-Neu5Ac, GM1,GD1b,GA1 to CMP, GD1a,GM1b,GT1b, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:47572.", "equation": "CMP-Neu5Ac + GM1,GD1b,GA1 → CMP + GD1a,GM1b,GT1b + H+", "inputs": [ { "node_id": "R-ALL-727815", "reactome_id": "R-ALL-727815", "label": "CMP-Neu5Ac", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57812", "formula": "C20H29N4O16P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9845510", "reactome_id": "R-ALL-9845510", "label": "GM1,GD1b,GA1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-727810", "reactome_id": "R-ALL-727810", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9845541", "reactome_id": "R-ALL-9845541", "label": "GD1a,GM1b,GT1b", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "beta-galactoside (CMP) alpha-2,3-sialyltransferase activity of ST3GAL2,3 [Golgi membrane]", "activity_id": "9845565", "ec_numbers": [ "2.4.3.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:8920913", "title": "Molecular cloning and expression of human Gal beta 1,3GalNAc alpha 2,3-sialytransferase (hST3Gal II)", "journal": "Biochem Biophys Res Commun", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8920913/" }, { "id": "PMID:22735313", "title": "Biosynthesis of the major brain gangliosides GD1a and GT1b", "journal": "Glycobiology", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22735313/" }, { "id": "PMID:34022416", "title": "Phenotype of ST3GAL3 deficient patients: A case and review of the literature", "journal": "Eur J Med Genet", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34022416/" }, { "id": "PMID:12815231", "title": "Cloning and sequencing of nineteen transcript isoforms of the human alpha2,3-sialyltransferase gene, ST3Gal III; its genomic organisation and expression in human tissues", "journal": "Glycoconj J", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12815231/" } ], "x": 3600, "y": 1660.173076923077, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9845587", "label": "ST8SIA5 transfers Neu5Ac to gangliosides", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9845587", "summary": "ST8SIA5 transfers Neu5Ac to gangliosides is a Reactome release 97 curated reaction event. The source record connects CMP-Neu5Ac, GM1b,GD1a,GD3,GT1b to CMP, GQ1b,GT3,GD1c,GT1a, H+ in Golgi membrane · Golgi lumen.", "equation": "CMP-Neu5Ac + GM1b,GD1a,GD3,GT1b → CMP + GQ1b,GT3,GD1c,GT1a + H+", "inputs": [ { "node_id": "R-ALL-727815", "reactome_id": "R-ALL-727815", "label": "CMP-Neu5Ac", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57812", "formula": "C20H29N4O16P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9845581", "reactome_id": "R-ALL-9845581", "label": "GM1b,GD1a,GD3,GT1b", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-727810", "reactome_id": "R-ALL-727810", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9845591", "reactome_id": "R-ALL-9845591", "label": "GQ1b,GT3,GD1c,GT1a", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alpha-N-acetylneuraminate alpha-2,8-sialyltransferase activity of ST8SIA5 [Golgi membrane]", "activity_id": "9845593", "ec_numbers": [ "2.4.3.8" ], "accessions": [ "O15466" ] } ], "citations": [ { "id": "PMID:9199191", "title": "Molecular cloning and expression of human alpha2,8-sialyltransferase (hST8Sia V)", "journal": "Biochem. Biophys. Res. Commun.", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9199191/" }, { "id": "PMID:8910600", "title": "Molecular cloning and expression of a fifth type of alpha2,8-sialyltransferase (ST8Sia V). Its substrate specificity is similar to that of SAT-V/III, which synthesize GD1c, GT1a, GQ1b and GT3", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8910600/" }, { "id": "PMID:34982351", "title": "Analysis of biochemical features of ST8 α-N-acetyl-neuraminide α2,8-sialyltransferase (St8sia) 5 isoforms", "journal": "Glycoconj J", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/34982351/" } ], "x": 3878.666666666667, "y": 1809.7884615384614, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9846305", "label": "ST6GALNAC5,6 transfer Neu5Ac to GM1b", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi lumen · Golgi membrane", "compartment_ids": [ "GO:0005796", "GO:0000139" ], "rhea_ids": [ "RHEA:41968" ], "source_url": "https://reactome.org/content/detail/R-HSA-9846305", "summary": "ST6GALNAC5,6 transfer Neu5Ac to GM1b is a Reactome release 97 curated reaction event. The source record connects GM1b, CMP-Neu5Ac to GD1alpha, CMP, H+ in Golgi lumen · Golgi membrane and cross-references RHEA:41968.", "equation": "GM1b + CMP-Neu5Ac → GD1alpha + CMP + H+", "inputs": [ { "node_id": "R-ALL-9845551", "reactome_id": "R-ALL-9845551", "label": "GM1b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78568", "formula": "C56H95N3O31R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-727815", "reactome_id": "R-ALL-727815", "label": "CMP-Neu5Ac", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57812", "formula": "C20H29N4O16P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9846309", "reactome_id": "R-ALL-9846309", "label": "GD1alpha", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78569", "formula": "C67H111N4O39R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-727810", "reactome_id": "R-ALL-727810", "label": "CMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60377", "formula": "C9H12N3O8P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase activity of ST6GALNAC5,6 [Golgi membrane]", "activity_id": "9846306", "ec_numbers": [ "2.4.3.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:12668675", "title": "Synthesis of disialyl Lewis a (Le(a)) structure in colon cancer cell lines by a sialyltransferase, ST6GalNAc VI, responsible for the synthesis of alpha-series gangliosides", "journal": "J. Biol. Chem.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12668675/" }, { "id": "PMID:10601645", "title": "A novel glycosyltransferase with a polyglutamine repeat; a new candidate for GD1alpha synthase (ST6GalNAc V)(1)", "journal": "FEBS Lett", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10601645/" } ], "x": 3321.3333333333335, "y": 1585.3653846153845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9846332", "label": "FUT1,2 transfer fucose to gangliosides", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi lumen · Golgi membrane", "compartment_ids": [ "GO:0005796", "GO:0000139" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9846332", "summary": "FUT1,2 transfer fucose to gangliosides is a Reactome release 97 curated reaction event. The source record connects GM1,GA1, GDP-Fuc to Fuc-GM1, Fuc-GA1, GDP, H+ in Golgi lumen · Golgi membrane.", "equation": "GM1,GA1 + GDP-Fuc → Fuc-GM1, Fuc-GA1 + GDP + H+", "inputs": [ { "node_id": "R-ALL-9846337", "reactome_id": "R-ALL-9846337", "label": "GM1,GA1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-205683", "reactome_id": "R-ALL-205683", "label": "GDP-Fuc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57273", "formula": "C16H23N5O15P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9846331", "reactome_id": "R-ALL-9846331", "label": "Fuc-GM1, Fuc-GA1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-205689", "reactome_id": "R-ALL-205689", "label": "GDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58189", "formula": "C10H12N5O11P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "fucosyltransferase activity of FUT1,2 [Golgi membrane]", "activity_id": "9846340", "ec_numbers": [ "2.4.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:2118655", "title": "Molecular cloning, sequence, and expression of a human GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase cDNA that can form the H blood group antigen", "journal": "Proc. Natl. Acad. Sci. U.S.A.", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2118655/" }, { "id": "PMID:7876235", "title": "Sequence and expression of a candidate for the human Secretor blood group alpha(1,2)fucosyltransferase gene (FUT2). Homozygosity for an enzyme-inactivating nonsense mutation commonly correlates with the non-secretor phenotype", "journal": "J. Biol. Chem.", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7876235/" } ], "x": 3321.3333333333335, "y": 1824.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9846477", "label": "A4GALT transfers galactose to LacCer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:11924" ], "source_url": "https://reactome.org/content/detail/R-HSA-9846477", "summary": "A4GALT transfers galactose to LacCer is a Reactome release 97 curated reaction event. The source record connects LacCer-d18, UDP-Gal to Gb3Cer, UDP, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:11924.", "equation": "LacCer-d18 + UDP-Gal → Gb3Cer + UDP + H+", "inputs": [ { "node_id": "R-ALL-9844874", "reactome_id": "R-ALL-9844874", "label": "LacCer-d18", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17950", "formula": "C31H56NO13R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-735682", "reactome_id": "R-ALL-735682", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8879102", "reactome_id": "R-ALL-8879102", "label": "Gb3Cer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18313", "formula": "C37H66NO18R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "lactosylceramide 4-alpha-galactosyltransferase activity of A4GALT [Golgi membrane]", "activity_id": "9846475", "ec_numbers": [ "2.4.1.228" ], "accessions": [ "Q9NPC4" ] } ], "citations": [ { "id": "PMID:10748143", "title": "Molecular cloning of globotriaosylceramide/CD77 synthase, a glycosyltransferase that initiates the synthesis of globo series glycosphingolipids", "journal": "J Biol Chem", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10748143/" }, { "id": "PMID:12888565", "title": "The blood group P1 synthase gene is identical to the Gb3/CD77 synthase gene. A clue to the solution of the P1/P2/p puzzle", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12888565/" } ], "x": 3600, "y": 1585.3653846153845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9846501", "label": "B3GNT5 transfers GlcNAc to LacCer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi lumen · Golgi membrane", "compartment_ids": [ "GO:0005796", "GO:0000139" ], "rhea_ids": [ "RHEA:13905" ], "source_url": "https://reactome.org/content/detail/R-HSA-9846501", "summary": "B3GNT5 transfers GlcNAc to LacCer is a Reactome release 97 curated reaction event. The source record connects LacCer-d18, UDP-GlcNAc to Lc3Cer, UDP, H+ in Golgi lumen · Golgi membrane and cross-references RHEA:13905.", "equation": "LacCer-d18 + UDP-GlcNAc → Lc3Cer + UDP + H+", "inputs": [ { "node_id": "R-ALL-9844874", "reactome_id": "R-ALL-9844874", "label": "LacCer-d18", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17950", "formula": "C31H56NO13R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-914003", "reactome_id": "R-ALL-914003", "label": "UDP-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9846503", "reactome_id": "R-ALL-9846503", "label": "Lc3Cer", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17103", "formula": "C39H69N2O18R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity of B3GNT5 [Golgi membrane]", "activity_id": "9846493", "ec_numbers": [ "2.4.1.206" ], "accessions": [ "Q9BYG0" ] } ], "citations": [ { "id": "PMID:11283017", "title": "Molecular cloning and characterization of UDP-GlcNAc:lactosylceramide beta 1,3-N-acetylglucosaminyltransferase (beta 3Gn-T5), an essential enzyme for the expression of HNK-1 and Lewis X epitopes on glycolipids", "journal": "J Biol Chem", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11283017/" }, { "id": "PMID:20547865", "title": "Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation", "journal": "Proc Natl Acad Sci U S A", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20547865/" } ], "x": 3878.666666666667, "y": 1585.3653846153845, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9851347", "label": "B4GALT5/6 transfer Gal to GlcCer", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:31495" ], "source_url": "https://reactome.org/content/detail/R-HSA-9851347", "summary": "B4GALT5/6 transfer Gal to GlcCer is a Reactome release 97 curated reaction event. The source record connects UDP-Gal, GlcCer to UDP, H+, LacCer-d18 in Golgi membrane · Golgi lumen and cross-references RHEA:31495.", "equation": "UDP-Gal + GlcCer → UDP + H+ + LacCer-d18", "inputs": [ { "node_id": "R-ALL-735682", "reactome_id": "R-ALL-735682", "label": "UDP-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:66914", "formula": "C15H22N2O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1660674", "reactome_id": "R-ALL-1660674", "label": "GlcCer", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:22801", "formula": "C25H46NO8R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9844874", "reactome_id": "R-ALL-9844874", "label": "LacCer-d18", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17950", "formula": "C31H56NO13R", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "UDP-galactose:glucosylceramide beta-1,4-galactosyltransferase activity of B4GALT5/6 [Golgi membrane]", "activity_id": "9845277", "ec_numbers": [ "2.4.1.274" ], "accessions": [] } ], "citations": [ { "id": "PMID:24498430", "title": "Establishment of HeLa cell mutants deficient in sphingolipid-related genes using TALENs", "journal": "PLoS One", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24498430/" }, { "id": "PMID:33673027", "title": "Convergence: Lactosylceramide-Centric Signaling Pathways Induce Inflammation, Oxidative Stress, and Other Phenotypic Outcomes", "journal": "Int J Mol Sci", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33673027/" }, { "id": "PMID:23882130", "title": "β4GalT6 is involved in the synthesis of lactosylceramide with less intensity than β4GalT5", "journal": "Glycobiology", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23882130/" } ], "x": 3321.3333333333335, "y": 1600.326923076923, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9853499", "label": "DLD dimer dehydrogenates dihydrolipoyl", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15046" ], "source_url": "https://reactome.org/content/detail/R-HSA-9853499", "summary": "DLD dimer dehydrogenates dihydrolipoyl is a Reactome release 97 curated reaction event. The source record connects NAD+, LipoH2-K110-DLST to H+, NADH, Lipo-K110-DLST in mitochondrial matrix and cross-references RHEA:15046.", "equation": "NAD+ + LipoH2-K110-DLST → H+ + NADH + Lipo-K110-DLST", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9853485", "reactome_id": "R-HSA-9853485", "label": "LipoH2-K110-DLST", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-69993", "reactome_id": "R-HSA-69993", "label": "Lipo-K110-DLST", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "dihydrolipoyl dehydrogenase (NADH) activity of OGDH complex [mitochondrial matrix]", "activity_id": "5694028", "ec_numbers": [ "1.8.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:15946682", "title": "Crystal structure of human dihydrolipoamide dehydrogenase: NAD+/NADH binding and the structural basis of disease-causing mutations", "journal": "J Mol Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15946682/" }, { "id": "PMID:28579060", "title": "Human dihydrolipoamide dehydrogenase (E3) deficiency: Novel insights into the structural basis and molecular pathomechanism", "journal": "Neurochem Int", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/28579060/" }, { "id": "PMID:26456061", "title": "Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components", "journal": "Free Radic Biol Med", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26456061/" }, { "id": "PMID:37701333", "title": "Biochemical characterization of patients with dihydrolipoamide dehydrogenase deficiency", "journal": "JIMD Rep", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37701333/" } ], "x": 5391.333333333333, "y": 481.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9853512", "label": "DLST transfers succinyl to CoA", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15215" ], "source_url": "https://reactome.org/content/detail/R-HSA-9853512", "summary": "DLST transfers succinyl to CoA is a Reactome release 97 curated reaction event. The source record connects CoA-SH, SuccinyllipoH2-K110-DLST to SUCC-CoA, LipoH2-K110-DLST in mitochondrial matrix and cross-references RHEA:15215.", "equation": "CoA-SH + SuccinyllipoH2-K110-DLST → SUCC-CoA + LipoH2-K110-DLST", "inputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9853502", "reactome_id": "R-HSA-9853502", "label": "SuccinyllipoH2-K110-DLST", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70958", "reactome_id": "R-ALL-70958", "label": "SUCC-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57292", "formula": "C25H35N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9853485", "reactome_id": "R-HSA-9853485", "label": "LipoH2-K110-DLST", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "dihydrolipoyllysine-residue succinyltransferase activity of OGDH complex [mitochondrial matrix]", "activity_id": "9853498", "ec_numbers": [ "2.3.1.61" ], "accessions": [] } ], "citations": [ { "id": "PMID:33684457", "title": "Structure of the dihydrolipoamide succinyltransferase (E2) component of the human alpha-ketoglutarate dehydrogenase complex (hKGDHc) revealed by cryo-EM and cross-linking mass spectrometry: Implications for the overall hKGDHc structure", "journal": "Biochim Biophys Acta Gen Subj", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33684457/" }, { "id": "PMID:30929736", "title": "Recurrent Germline DLST Mutations in Individuals with Multiple Pheochromocytomas and Paragangliomas", "journal": "Am J Hum Genet", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30929736/" }, { "id": "PMID:8076640", "title": "Isolation, characterization and structural organization of the gene and pseudogene for the dihydrolipoamide succinyltransferase component of the human 2-oxoglutarate dehydrogenase complex", "journal": "Eur J Biochem", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/8076640/" } ], "x": 5391.333333333333, "y": 530.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9854315", "label": "CSKMT methylates Citrate Synthase", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:55544", "RHEA:55548", "RHEA:55552" ], "source_url": "https://reactome.org/content/detail/R-HSA-9854315", "summary": "CSKMT methylates Citrate Synthase is a Reactome release 97 curated reaction event. The source record connects Methylated(n) CS dimer, S-adenosyl-L-methionine to Methylated(n+1) CS dimer, H+, S-adenosyl-L-homocysteine in mitochondrial matrix and cross-references RHEA:55544, RHEA:55548, RHEA:55552.", "equation": "Methylated(n) CS dimer + 2 S-adenosyl-L-methionine → Methylated(n+1) CS dimer + 2 H+ + 2 S-adenosyl-L-homocysteine", "inputs": [ { "node_id": "R-HSA-9854325", "reactome_id": "R-HSA-9854325", "label": "Methylated(n) CS dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-9854319", "reactome_id": "R-ALL-9854319", "label": "S-adenosyl-L-methionine", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:59789", "formula": "C15H23N6O5S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9854313", "reactome_id": "R-HSA-9854313", "label": "Methylated(n+1) CS dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9854312", "reactome_id": "R-ALL-9854312", "label": "S-adenosyl-L-homocysteine", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57856", "formula": "C14H20N6O5S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "protein-lysine N-methyltransferase activity of CSKMT [mitochondrial matrix]", "activity_id": "9854316", "ec_numbers": [ "2.1.1" ], "accessions": [ "A8MUP2" ] } ], "citations": [ { "id": "PMID:28887308", "title": "Uncovering human METTL12 as a mitochondrial methyltransferase that modulates citrate synthase activity through metabolite-sensitive lysine methylation", "journal": "J Biol Chem", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28887308/" }, { "id": "PMID:28391595", "title": "Human METTL12 is a mitochondrial methyltransferase that modifies citrate synthase", "journal": "FEBS Lett", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28391595/" }, { "id": "PMID:37533128", "title": "Methyltransferase-like proteins in cancer biology and potential therapeutic targeting", "journal": "J Hematol Oncol", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37533128/" } ], "x": 5391.333333333333, "y": 287.3125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9854368", "label": "ROS,RNS oxidize ACO2:4Fe-4S", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9854368", "summary": "ROS,RNS oxidize ACO2:4Fe-4S is a Reactome release 97 curated reaction event. The source record connects ROS,RNS, ACO2:4Fe-4S to ACO2:3Fe-4S, Fe2+ in mitochondrial matrix.", "equation": "ROS,RNS + ACO2:4Fe-4S → ACO2:3Fe-4S + Fe2+", "inputs": [ { "node_id": "R-ALL-9854374", "reactome_id": "R-ALL-9854374", "label": "ROS,RNS", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-9854344", "reactome_id": "R-HSA-9854344", "label": "ACO2:4Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9854373", "reactome_id": "R-HSA-9854373", "label": "ACO2:3Fe-4S", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113531", "reactome_id": "R-ALL-113531", "label": "Fe2+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "molecular sensor activity of ACO2:4Fe-4S [mitochondrial matrix]", "activity_id": "9956929", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:1315737", "title": "Inactivation-reactivation of aconitase in Escherichia coli. A sensitive measure of superoxide radical", "journal": "J Biol Chem", "year": 1992, "url": "https://pubmed.ncbi.nlm.nih.gov/1315737/" }, { "id": "PMID:17349934", "title": "Mitochondrial aconitase reaction with nitric oxide, S-nitrosoglutathione, and peroxynitrite: mechanisms and relative contributions to aconitase inactivation", "journal": "Free Radic Biol Med", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17349934/" }, { "id": "PMID:11848830", "title": "Aconitase as Iron-Sulfur Protein, Enzyme, and Iron-Regulatory Protein", "journal": "Chem Rev", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/11848830/" } ], "x": 5670, "y": 530.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9854405", "label": "Frataxin transfers Fe2+ to ACO2", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9854405", "summary": "Frataxin transfers Fe2+ to ACO2 is a Reactome release 97 curated reaction event. The source record connects ACO2:3Fe-4S, 2 Iron:FXN to ACO2:4Fe-4S, FXN(81-210), Fe2+ in mitochondrial matrix.", "equation": "ACO2:3Fe-4S + 2 Iron:FXN → ACO2:4Fe-4S + FXN(81-210) + Fe2+", "inputs": [ { "node_id": "R-HSA-9854373", "reactome_id": "R-HSA-9854373", "label": "ACO2:3Fe-4S", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1362402", "reactome_id": "R-HSA-1362402", "label": "2 Iron:FXN", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9854344", "reactome_id": "R-HSA-9854344", "label": "ACO2:4Fe-4S", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1362390", "reactome_id": "R-HSA-1362390", "label": "FXN(81-210)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113531", "reactome_id": "R-ALL-113531", "label": "Fe2+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "iron chaperone activity of 2 Iron:FXN [mitochondrial matrix]", "activity_id": "9855646", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:36738801", "title": "Redox sensitive human mitochondrial aconitase and its interaction with frataxin: In vitro and in silico studies confirm that it takes two to tango", "journal": "Free Radic Biol Med", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36738801/" }, { "id": "PMID:15247478", "title": "Frataxin acts as an iron chaperone protein to modulate mitochondrial aconitase activity", "journal": "Science", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15247478/" }, { "id": "PMID:9989622", "title": "Friedreich's ataxia: point mutations and clinical presentation of compound heterozygotes", "journal": "Ann Neurol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/9989622/" } ], "x": 5948.666666666667, "y": 530.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9854415", "label": "ACAT1 tetramer acetylates IDH2 dimer", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:45948" ], "source_url": "https://reactome.org/content/detail/R-HSA-9854415", "summary": "ACAT1 tetramer acetylates IDH2 dimer is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, IDH2 dimer to CoA-SH, H+, AcK-IDH2 dimer in mitochondrial matrix and cross-references RHEA:45948.", "equation": "2 Ac-CoA + IDH2 dimer → 2 CoA-SH + 2 H+ + AcK-IDH2 dimer", "inputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-450982", "reactome_id": "R-HSA-450982", "label": "IDH2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9854462", "reactome_id": "R-HSA-9854462", "label": "AcK-IDH2 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "acetyltransferase activity of ACAT1 tetramer [mitochondrial matrix]", "activity_id": "9854426", "ec_numbers": [ "2.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:20167786", "title": "Regulation of cellular metabolism by protein lysine acetylation", "journal": "Science", "year": 2010, "url": "https://pubmed.ncbi.nlm.nih.gov/20167786/" }, { "id": "PMID:34289383", "title": "Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells", "journal": "Mol Cell", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34289383/" } ], "x": 5670, "y": 481.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9854463", "label": "SIRT3 deacetylates AcK-IDH2 dimer", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:43636" ], "source_url": "https://reactome.org/content/detail/R-HSA-9854463", "summary": "SIRT3 deacetylates AcK-IDH2 dimer is a Reactome release 97 curated reaction event. The source record connects NAD+, AcK-IDH2 dimer to NAM, 2'-O-acetyl-ADP-ribose, IDH2 dimer in mitochondrial matrix and cross-references RHEA:43636.", "equation": "2 NAD+ + AcK-IDH2 dimer → 2 NAM + 2 2'-O-acetyl-ADP-ribose + IDH2 dimer", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9854462", "reactome_id": "R-HSA-9854462", "label": "AcK-IDH2 dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5688274", "reactome_id": "R-ALL-5688274", "label": "NAM", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5688337", "reactome_id": "R-ALL-5688337", "label": "2'-O-acetyl-ADP-ribose", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:83767", "formula": "C17H23N5O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-450982", "reactome_id": "R-HSA-450982", "label": "IDH2 dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "NAD-dependent protein lysine deacetylase activity of SIRT3(?-399):Zn2+ [mitochondrial matrix]", "activity_id": "5688302", "ec_numbers": [ "2.3.1.286" ], "accessions": [] } ], "citations": [ { "id": "PMID:17923681", "title": "Mammalian Sir2 homolog SIRT3 regulates global mitochondrial lysine acetylation", "journal": "Mol. Cell. Biol.", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17923681/" }, { "id": "PMID:22416140", "title": "SIRT3 protein deacetylates isocitrate dehydrogenase 2 (IDH2) and regulates mitochondrial redox status", "journal": "J Biol Chem", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22416140/" }, { "id": "PMID:34289383", "title": "Lysine acetylation restricts mutant IDH2 activity to optimize transformation in AML cells", "journal": "Mol Cell", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34289383/" }, { "id": "PMID:37237500", "title": "Sirtuin-3-Mediated Cellular Metabolism Links Cardiovascular Remodeling with Hypertension", "journal": "Biology (Basel)", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37237500/" } ], "x": 5948.666666666667, "y": 481.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9854672", "label": "FAD attaches to SDHA", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9854672", "summary": "FAD attaches to SDHA is a Reactome release 97 curated reaction event. The source record connects SDHA, FAD to FAD-SDHA in mitochondrial matrix.", "equation": "SDHA + FAD → FAD-SDHA", "inputs": [ { "node_id": "R-HSA-9854690", "reactome_id": "R-HSA-9854690", "label": "SDHA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113596", "reactome_id": "R-ALL-113596", "label": "FAD", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57692", "formula": "C27H30N9O15P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-70983", "reactome_id": "R-HSA-70983", "label": "FAD-SDHA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "FAD binding activity of SDHA [mitochondrial matrix]", "activity_id": "9956931", "ec_numbers": null, "accessions": [ "P31040" ] } ], "citations": [ { "id": "PMID:36089066", "title": "How an assembly factor enhances covalent FAD attachment to the flavoprotein subunit of complex II", "journal": "J Biol Chem", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36089066/" }, { "id": "PMID:19628817", "title": "SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma", "journal": "Science", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19628817/" } ], "x": 5391.333333333333, "y": 579.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9854961", "label": "SDHAF4 binds to FAD-SDHA", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9854961", "summary": "SDHAF4 binds to FAD-SDHA is a Reactome release 97 curated reaction event. The source record connects FAD-SDHA, SDHAF4 to FAD-SDHA:SDHAF4 in mitochondrial matrix.", "equation": "FAD-SDHA + SDHAF4 → FAD-SDHA:SDHAF4", "inputs": [ { "node_id": "R-HSA-70983", "reactome_id": "R-HSA-70983", "label": "FAD-SDHA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9854972", "reactome_id": "R-HSA-9854972", "label": "SDHAF4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9854960", "reactome_id": "R-HSA-9854960", "label": "FAD-SDHA:SDHAF4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24954416", "title": "SDHAF4 promotes mitochondrial succinate dehydrogenase activity and prevents neurodegeneration", "journal": "Cell Metab", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24954416/" }, { "id": "PMID:35037426", "title": "Hepatic Suppression of Mitochondrial Complex II Assembly Drives Systemic Metabolic Benefits", "journal": "Adv Sci (Weinh)", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35037426/" } ], "x": 5948.666666666667, "y": 579.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9854984", "label": "Transfer of Fe-S clusters to SDHB", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9854984", "summary": "Transfer of Fe-S clusters to SDHB is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster, ISCA1:ISCA2:4Fe-4S, SDHB to FXN:ISD11:NFS1:ISCU, ISCA1:ISCA2, Fe2+ in mitochondrial matrix.", "equation": "FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster + 2 ISCA1:ISCA2:4Fe-4S + SDHB + SDHAF1 + SDHAF3 → FXN:ISD11:NFS1:ISCU + 2 ISCA1:ISCA2 + Fe2+ + SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHAF1:SDHAF3", "inputs": [ { "node_id": "R-HSA-1362398", "reactome_id": "R-HSA-1362398", "label": "FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-8878796", "reactome_id": "R-HSA-8878796", "label": "ISCA1:ISCA2:4Fe-4S", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9854983", "reactome_id": "R-HSA-9854983", "label": "SDHB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9854988", "reactome_id": "R-HSA-9854988", "label": "SDHAF1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9855015", "reactome_id": "R-HSA-9855015", "label": "SDHAF3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1362404", "reactome_id": "R-HSA-1362404", "label": "FXN:ISD11:NFS1:ISCU", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-8878840", "reactome_id": "R-HSA-8878840", "label": "ISCA1:ISCA2", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-113531", "reactome_id": "R-ALL-113531", "label": "Fe2+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:29033", "formula": "Fe", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9854993", "reactome_id": "R-HSA-9854993", "label": "SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHAF1:SDHAF3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "iron chaperone activity of 2 Iron:FXN [mitochondrial matrix]", "activity_id": "9855646", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:25488574", "title": "Protein-mediated assembly of succinate dehydrogenase and its cofactors", "journal": "Crit Rev Biochem Mol Biol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25488574/" }, { "id": "PMID:28380382", "title": "A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III", "journal": "Cell Metab", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28380382/" }, { "id": "PMID:26749241", "title": "Disease-Causing SDHAF1 Mutations Impair Transfer of Fe-S Clusters to SDHB", "journal": "Cell Metab", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26749241/" }, { "id": "PMID:24954417", "title": "The LYR factors SDHAF1 and SDHAF3 mediate maturation of the iron-sulfur subunit of succinate dehydrogenase", "journal": "Cell Metab", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24954417/" }, { "id": "PMID:30030361", "title": "Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes", "journal": "Essays Biochem", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30030361/" } ], "x": 5670, "y": 627.6875, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9855212", "label": "SDHA binds to SDHB", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9855212", "summary": "SDHA binds to SDHB is a Reactome release 97 curated reaction event. The source record connects FAD-SDHA:SDHAF4, SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHAF1:SDHAF3 to SDHAF4, FAD-SDHA:SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHFA1:SDHFA3 in mitochondrial matrix.", "equation": "FAD-SDHA:SDHAF4 + SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHAF1:SDHAF3 → SDHAF4 + FAD-SDHA:SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHFA1:SDHFA3", "inputs": [ { "node_id": "R-HSA-9854960", "reactome_id": "R-HSA-9854960", "label": "FAD-SDHA:SDHAF4", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9854993", "reactome_id": "R-HSA-9854993", "label": "SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHAF1:SDHAF3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9854972", "reactome_id": "R-HSA-9854972", "label": "SDHAF4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9855213", "reactome_id": "R-HSA-9855213", "label": "FAD-SDHA:SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHFA1:SDHFA3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein folding chaperone activity of SDHAF4 [mitochondrial matrix]", "activity_id": "9861211", "ec_numbers": null, "accessions": [ "Q5VUM1" ] } ], "citations": [ { "id": "PMID:25488574", "title": "Protein-mediated assembly of succinate dehydrogenase and its cofactors", "journal": "Crit Rev Biochem Mol Biol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25488574/" }, { "id": "PMID:24954417", "title": "The LYR factors SDHAF1 and SDHAF3 mediate maturation of the iron-sulfur subunit of succinate dehydrogenase", "journal": "Cell Metab", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24954417/" } ], "x": 5670, "y": 579.0625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9855252", "label": "SDHA:SDHB binds to SDHC:SDHD", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "tca" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9855252", "summary": "SDHA:SDHB binds to SDHC:SDHD is a Reactome release 97 curated reaction event. The source record connects FAD-SDHA:SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHFA1:SDHFA3, SDHC:SDHD:heme b to SDH complex, SDHAF1, SDHAF3 in mitochondrial inner membrane · mitochondrial matrix.", "equation": "FAD-SDHA:SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHFA1:SDHFA3 + SDHC:SDHD:heme b → SDH complex + SDHAF1 + SDHAF3", "inputs": [ { "node_id": "R-HSA-9855213", "reactome_id": "R-HSA-9855213", "label": "FAD-SDHA:SDHB:2Fe-2S:3Fe-4S:4Fe-4S:SDHFA1:SDHFA3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9853501", "reactome_id": "R-HSA-9853501", "label": "SDHC:SDHD:heme b", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-70990", "reactome_id": "R-HSA-70990", "label": "SDH complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9854988", "reactome_id": "R-HSA-9854988", "label": "SDHAF1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9855015", "reactome_id": "R-HSA-9855015", "label": "SDHAF3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:30030361", "title": "Assembly of mammalian oxidative phosphorylation complexes I-V and supercomplexes", "journal": "Essays Biochem", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30030361/" }, { "id": "PMID:25488574", "title": "Protein-mediated assembly of succinate dehydrogenase and its cofactors", "journal": "Crit Rev Biochem Mol Biol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25488574/" }, { "id": "PMID:23043141", "title": "Flavinylation and assembly of succinate dehydrogenase are dependent on the C-terminal tail of the flavoprotein subunit", "journal": "J Biol Chem", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23043141/" }, { "id": "PMID:19332149", "title": "Mutations in the heme b-binding residue of SDHC inhibit assembly of respiratory chain complex II in mammalian cells", "journal": "Mitochondrion", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19332149/" } ], "x": 5391.333333333333, "y": 627.6875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9856502", "label": "mito-STARD7 transports Q10 to outer mitochondrial membrane", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial inner membrane · mitochondrial outer membrane", "compartment_ids": [ "GO:0005743", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9856502", "summary": "mito-STARD7 transports Q10 to outer mitochondrial membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CoQH2 to CoQH2 in mitochondrial inner membrane · mitochondrial outer membrane.", "equation": "CoQH2 → CoQH2", "inputs": [ { "node_id": "R-ALL-2162198", "reactome_id": "R-ALL-2162198", "label": "CoQH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9856515", "reactome_id": "R-ALL-9856515", "label": "CoQH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lipid transfer activity of mito-STARD7 [mitochondrial outer membrane]", "activity_id": "9856507", "ec_numbers": null, "accessions": [ "Q9NQZ5" ] } ], "citations": [ { "id": "PMID:36658222", "title": "Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7", "journal": "Nat Cell Biol", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36658222/" }, { "id": "PMID:37507930", "title": "New Insights on the Uptake and Trafficking of Coenzyme Q", "journal": "Antioxidants (Basel)", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37507930/" }, { "id": "PMID:34362905", "title": "UbiB proteins regulate cellular CoQ distribution in Saccharomyces cerevisiae", "journal": "Nat Commun", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34362905/" } ], "x": 5008.666666666667, "y": 3525.125, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9856510", "label": "STARD7 transports Q10 to plasma membrane", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "plasma membrane · mitochondrial outer membrane", "compartment_ids": [ "GO:0005886", "GO:0005741" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9856510", "summary": "STARD7 transports Q10 to plasma membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CoQH2 to CoQH2 in plasma membrane · mitochondrial outer membrane.", "equation": "CoQH2 → CoQH2", "inputs": [ { "node_id": "R-ALL-9856515", "reactome_id": "R-ALL-9856515", "label": "CoQH2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9856514", "reactome_id": "R-ALL-9856514", "label": "CoQH2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64183", "formula": "C59H92O4", "compartment": "plasma membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "lipid transfer activity of cytosolic STARD7 [mitochondrial outer membrane]", "activity_id": "9856505", "ec_numbers": null, "accessions": [ "Q9NQZ5" ] } ], "citations": [ { "id": "PMID:36658222", "title": "Mitochondria regulate intracellular coenzyme Q transport and ferroptotic resistance via STARD7", "journal": "Nat Cell Biol", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36658222/" }, { "id": "PMID:37507930", "title": "New Insights on the Uptake and Trafficking of Coenzyme Q", "journal": "Antioxidants (Basel)", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37507930/" } ], "x": 4451.333333333333, "y": 3557.5416666666665, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9856718", "label": "HPDL dioxygenates HPPA", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:21377" ], "source_url": "https://reactome.org/content/detail/R-HSA-9856718", "summary": "HPDL dioxygenates HPPA is a Reactome release 97 curated reaction event. The source record connects HPP, O2 to 4-HMA, CO2 in mitochondrial matrix and cross-references RHEA:21377.", "equation": "HPP + O2 → 4-HMA + CO2", "inputs": [ { "node_id": "R-ALL-9856684", "reactome_id": "R-ALL-9856684", "label": "HPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36242", "formula": "C9H7O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113533", "reactome_id": "R-ALL-113533", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9856722", "reactome_id": "R-ALL-9856722", "label": "4-HMA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17210", "formula": "C8H7O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "4-hydroxymandelate synthase activity of HPDL:Fe2+ [mitochondrial matrix]", "activity_id": "9856680", "ec_numbers": [ "1.13.11.46" ], "accessions": [] } ], "citations": [ { "id": "PMID:37508007", "title": "Biosynthesis, Deficiency, and Supplementation of Coenzyme Q", "journal": "Antioxidants (Basel)", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37508007/" }, { "id": "PMID:34471290", "title": "The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 synthesis pathway", "journal": "Nature", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34471290/" }, { "id": "PMID:32707086", "title": "Bi-allelic HPDL Variants Cause a Neurodegenerative Disease Ranging from Neonatal Encephalopathy to Adolescent-Onset Spastic Paraplegia", "journal": "Am J Hum Genet", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32707086/" } ], "x": 4451.333333333333, "y": 3363.0416666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9856737", "label": "Unknown enzyme(s) process 4-HMA to 4-Hbz", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9856737", "summary": "Unknown enzyme(s) process 4-HMA to 4-Hbz is a Reactome release 97 curated reaction event. The source record connects 4-HMA to 4-Hbz in mitochondrial matrix.", "equation": "4-HMA → 4-Hbz", "inputs": [ { "node_id": "R-ALL-9856722", "reactome_id": "R-ALL-9856722", "label": "4-HMA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17210", "formula": "C8H7O4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9856800", "reactome_id": "R-ALL-9856800", "label": "4-Hbz", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17597", "formula": "C7H6O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carboxy-lyase activity of Unknown carboxylyase [mitochondrial matrix]", "activity_id": "9949579", "ec_numbers": [ "4.1.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:37508007", "title": "Biosynthesis, Deficiency, and Supplementation of Coenzyme Q", "journal": "Antioxidants (Basel)", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37508007/" }, { "id": "PMID:34471290", "title": "The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 synthesis pathway", "journal": "Nature", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34471290/" }, { "id": "PMID:37863926", "title": "Lactate dehydrogenase D is a general dehydrogenase for D-2-hydroxyacids and is associated with D-lactic acidosis", "journal": "Nat Commun", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37863926/" }, { "id": "PMID:33431826", "title": "Structure, substrate specificity, and catalytic mechanism of human D-2-HGDH and insights into pathogenicity of disease-associated mutations", "journal": "Cell Discov", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33431826/" }, { "id": "PMID:32561586", "title": "The Mandelate Pathway, an Alternative to the Phenylalanine Ammonia Lyase Pathway for the Synthesis of Benzenoids in Ascomycete Yeasts", "journal": "Appl Environ Microbiol", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32561586/" }, { "id": "PMID:34198496", "title": "Coenzyme Q Biosynthesis: An Update on the Origins of the Benzenoid Ring and Discovery of New Ring Precursors", "journal": "Metabolites", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34198496/" } ], "x": 4730, "y": 3557.5416666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9856794", "label": "Unknown dehydrogenase oxidizes 4-HBz", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "cofactor-metabolism" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:20306" ], "source_url": "https://reactome.org/content/detail/R-HSA-9856794", "summary": "Unknown dehydrogenase oxidizes 4-HBz is a Reactome release 97 curated reaction event. The source record connects 4-Hbz, H2O, NAD+ to PHB, NADH, H+ in mitochondrial matrix and cross-references RHEA:20306.", "equation": "4-Hbz + H2O + NAD+ → PHB + NADH + H+", "inputs": [ { "node_id": "R-ALL-9856800", "reactome_id": "R-ALL-9856800", "label": "4-Hbz", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:17597", "formula": "C7H6O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2162213", "reactome_id": "R-ALL-2162213", "label": "PHB", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17879", "formula": "C7H5O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "4-hydroxybenzaldehyde dehydrogenase (NAD+) activity of Unknown dehydrogenase [mitochondrial matrix]", "activity_id": "9856735", "ec_numbers": [ "1.2.1.64" ], "accessions": [] } ], "citations": [ { "id": "PMID:27693056", "title": "Mechanistic Details of Early Steps in Coenzyme Q Biosynthesis Pathway in Yeast", "journal": "Cell Chem Biol", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27693056/" }, { "id": "PMID:27669165", "title": "Mitochondrial protein functions elucidated by multi-omic mass spectrometry profiling", "journal": "Nat Biotechnol", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27669165/" } ], "x": 4451.333333333333, "y": 3298.2083333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9856871", "label": "MDH1 reduces OA", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport", "malate-aspartate-shuttle" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:21434" ], "source_url": "https://reactome.org/content/detail/R-HSA-9856871", "summary": "MDH1 reduces OA is a Reactome release 97 curated reaction event. The source record connects NADH, OA, H+ to MAL, NAD+ in cytosol and cross-references RHEA:21434.", "equation": "NADH + OA + H+ → MAL + NAD+", "inputs": [ { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-76213", "reactome_id": "R-ALL-76213", "label": "OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-198498", "reactome_id": "R-ALL-198498", "label": "MAL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15589", "formula": "C4H4O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-malate dehydrogenase (NAD+) activity of MDH1 dimer [cytosol]", "activity_id": "198512", "ec_numbers": [ "1.1.1.37" ], "accessions": [] } ], "citations": [ { "id": "PMID:2775751", "title": "Refined crystal structure of cytoplasmic malate dehydrogenase at 2.5-A resolution", "journal": "Biochemistry", "year": 1989, "url": "https://pubmed.ncbi.nlm.nih.gov/2775751/" }, { "id": "PMID:3052244", "title": "Biochemical and genetic identity of alpha-keto acid reductase and cytoplasmic malate dehydrogenase from human erythrocytes", "journal": "Ann Hum Genet", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3052244/" }, { "id": "PMID:15565635", "title": "Developmental regulation and cellular distribution of human cytosolic malate dehydrogenase (MDH1)", "journal": "J Cell Biochem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15565635/" }, { "id": "PMID:22971926", "title": "Cytosolic malate dehydrogenase regulates senescence in human fibroblasts", "journal": "Biogerontology", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22971926/" }, { "id": "PMID:31538237", "title": "MDH1 deficiency is a metabolic disorder of the malate-aspartate shuttle associated with early onset severe encephalopathy", "journal": "Hum Genet", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31538237/" }, { "id": "PMID:35036692", "title": "Structural Characterization of the Human Cytosolic Malate Dehydrogenase I", "journal": "ACS Omega", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35036692/" } ], "x": 4451.333333333333, "y": 754.9666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9858321", "label": "DHTKD1 dimer decarboxylates 2-OA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:69577" ], "source_url": "https://reactome.org/content/detail/R-HSA-9858321", "summary": "DHTKD1 dimer decarboxylates 2-OA is a Reactome release 97 curated reaction event. The source record connects H+, Lipo-K110-DLST, 2-OA to CO2, GlutaryllipoH2-K110-DLST in mitochondrial matrix and cross-references RHEA:69577.", "equation": "H+ + Lipo-K110-DLST + 2-OA → CO2 + GlutaryllipoH2-K110-DLST", "inputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-69993", "reactome_id": "R-HSA-69993", "label": "Lipo-K110-DLST", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-70951", "reactome_id": "R-ALL-70951", "label": "2-OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57499", "formula": "C6H6O5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9858581", "reactome_id": "R-HSA-9858581", "label": "GlutaryllipoH2-K110-DLST", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor of OADH complex [mitochondrial matrix]", "activity_id": "9853486", "ec_numbers": [ "1.2.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:29191460", "title": "The mitochondrial 2-oxoadipate and 2-oxoglutarate dehydrogenase complexes share their E2 and E3 components for their function and both generate reactive oxygen species", "journal": "Free Radic Biol Med", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29191460/" }, { "id": "PMID:32695416", "title": "Crystal structure and interaction studies of human DHTKD1 provide insight into a mitochondrial megacomplex in lysine catabolism", "journal": "IUCrJ", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32695416/" }, { "id": "PMID:32633484", "title": "Inhibition and Crystal Structure of the Human DHTKD1-Thiamin Diphosphate Complex", "journal": "ACS Chem Biol", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32633484/" }, { "id": "PMID:23141294", "title": "A nonsense mutation in DHTKD1 causes Charcot-Marie-Tooth disease type 2 in a large Chinese pedigree", "journal": "Am J Hum Genet", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23141294/" }, { "id": "PMID:25860818", "title": "Genetic basis of alpha-aminoadipic and alpha-ketoadipic aciduria", "journal": "J Inherit Metab Dis", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25860818/" }, { "id": "PMID:35052424", "title": "Heterozygous DHTKD1 Variants in Two European Cohorts of Amyotrophic Lateral Sclerosis Patients", "journal": "Genes (Basel)", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/35052424/" } ], "x": 6521.333333333333, "y": 2124.125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9858589", "label": "DLD dimer dehydrogenates dihydrolipoyl", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15046" ], "source_url": "https://reactome.org/content/detail/R-HSA-9858589", "summary": "DLD dimer dehydrogenates dihydrolipoyl is a Reactome release 97 curated reaction event. The source record connects NAD+, LipoH2-K110-DLST to H+, NADH, Lipo-K110-DLST in mitochondrial matrix and cross-references RHEA:15046.", "equation": "NAD+ + LipoH2-K110-DLST → H+ + NADH + Lipo-K110-DLST", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9853485", "reactome_id": "R-HSA-9853485", "label": "LipoH2-K110-DLST", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-69993", "reactome_id": "R-HSA-69993", "label": "Lipo-K110-DLST", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "dihydrolipoyl dehydrogenase (NADH) activity of OADH complex [mitochondrial matrix]", "activity_id": "9858592", "ec_numbers": [ "1.8.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:15946682", "title": "Crystal structure of human dihydrolipoamide dehydrogenase: NAD+/NADH binding and the structural basis of disease-causing mutations", "journal": "J Mol Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15946682/" }, { "id": "PMID:28579060", "title": "Human dihydrolipoamide dehydrogenase (E3) deficiency: Novel insights into the structural basis and molecular pathomechanism", "journal": "Neurochem Int", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/28579060/" }, { "id": "PMID:26456061", "title": "Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components", "journal": "Free Radic Biol Med", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26456061/" }, { "id": "PMID:37701333", "title": "Biochemical characterization of patients with dihydrolipoamide dehydrogenase deficiency", "journal": "JIMD Rep", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37701333/" }, { "id": "PMID:29191460", "title": "The mitochondrial 2-oxoadipate and 2-oxoglutarate dehydrogenase complexes share their E2 and E3 components for their function and both generate reactive oxygen species", "journal": "Free Radic Biol Med", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29191460/" } ], "x": 6800, "y": 2124.125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9858590", "label": "DLST transfers glutaryl to CoA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18011" ], "source_url": "https://reactome.org/content/detail/R-HSA-9858590", "summary": "DLST transfers glutaryl to CoA is a Reactome release 97 curated reaction event. The source record connects CoA-SH, GlutaryllipoH2-K110-DLST, H+ to LipoH2-K110-DLST, GL-CoA in mitochondrial matrix and cross-references RHEA:18011.", "equation": "CoA-SH + GlutaryllipoH2-K110-DLST + H+ → LipoH2-K110-DLST + GL-CoA", "inputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9858581", "reactome_id": "R-HSA-9858581", "label": "GlutaryllipoH2-K110-DLST", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-9853485", "reactome_id": "R-HSA-9853485", "label": "LipoH2-K110-DLST", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-70955", "reactome_id": "R-ALL-70955", "label": "GL-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57378", "formula": "C26H37N7O19P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "peptide glutaryltransferase activity of OADH complex [mitochondrial matrix]", "activity_id": "9858591", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:30929736", "title": "Recurrent Germline DLST Mutations in Individuals with Multiple Pheochromocytomas and Paragangliomas", "journal": "Am J Hum Genet", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30929736/" }, { "id": "PMID:29191460", "title": "The mitochondrial 2-oxoadipate and 2-oxoglutarate dehydrogenase complexes share their E2 and E3 components for their function and both generate reactive oxygen species", "journal": "Free Radic Biol Med", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29191460/" } ], "x": 7078.666666666667, "y": 2124.125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9859148", "label": "BCKDHA:BCKDHB tetramer decarboxylates KIC, KMVA, KIV", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:13458" ], "source_url": "https://reactome.org/content/detail/R-HSA-9859148", "summary": "BCKDHA:BCKDHB tetramer decarboxylates KIC, KMVA, KIV is a Reactome release 97 curated reaction event. The source record connects Lipo-K105-DBT, KIC, KMVA, KIV, H+ to CO2, BCAA-lipoH2-K105-DBT in mitochondrial matrix and cross-references RHEA:13458.", "equation": "Lipo-K105-DBT + KIC, KMVA, KIV + H+ → CO2 + BCAA-lipoH2-K105-DBT", "inputs": [ { "node_id": "R-HSA-70017", "reactome_id": "R-HSA-70017", "label": "Lipo-K105-DBT", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-508181", "reactome_id": "R-ALL-508181", "label": "KIC, KMVA, KIV", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9859149", "reactome_id": "R-HSA-9859149", "label": "BCAA-lipoH2-K105-DBT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor of BCKDH [mitochondrial matrix]", "activity_id": "9859137", "ec_numbers": [ "1.2.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:10745006", "title": "Crystal structure of human branched-chain alpha-ketoacid dehydrogenase and the molecular basis of multienzyme complex deficiency in maple syrup urine disease.", "journal": "Structure Fold Des", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10745006/" } ], "x": 6800, "y": 1674.1944444444443, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9859163", "label": "DBT transfers BCAA to CoA", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:18867" ], "source_url": "https://reactome.org/content/detail/R-HSA-9859163", "summary": "DBT transfers BCAA to CoA is a Reactome release 97 curated reaction event. The source record connects BCAA-lipoH2-K105-DBT, CoA-SH to BCAA-CoAs, LipoH2-K105-DBT in mitochondrial matrix and cross-references RHEA:18867.", "equation": "BCAA-lipoH2-K105-DBT + CoA-SH → BCAA-CoAs + LipoH2-K105-DBT", "inputs": [ { "node_id": "R-HSA-9859149", "reactome_id": "R-HSA-9859149", "label": "BCAA-lipoH2-K105-DBT", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-508261", "reactome_id": "R-ALL-508261", "label": "BCAA-CoAs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-9859167", "reactome_id": "R-HSA-9859167", "label": "LipoH2-K105-DBT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "acyltransferase activity, transferring groups other than amino-acyl groups of BCKDH [mitochondrial matrix]", "activity_id": "9859164", "ec_numbers": [ "2.3.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:35799415", "title": "Genomic and biochemical analysis of repeatedly observed variants in DBT in individuals with maple syrup urine disease of Central American ancestry", "journal": "Am J Med Genet A", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35799415/" } ], "x": 7078.666666666667, "y": 1674.1944444444443, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9859172", "label": "DLD dimer dehydrogenates dihydrolipoyl", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15046" ], "source_url": "https://reactome.org/content/detail/R-HSA-9859172", "summary": "DLD dimer dehydrogenates dihydrolipoyl is a Reactome release 97 curated reaction event. The source record connects NAD+, LipoH2-K105-DBT to H+, NADH, Lipo-K105-DBT in mitochondrial matrix and cross-references RHEA:15046.", "equation": "NAD+ + LipoH2-K105-DBT → H+ + NADH + Lipo-K105-DBT", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9859167", "reactome_id": "R-HSA-9859167", "label": "LipoH2-K105-DBT", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-70017", "reactome_id": "R-HSA-70017", "label": "Lipo-K105-DBT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "dihydrolipoyl dehydrogenase (NADH) activity of BCKDH [mitochondrial matrix]", "activity_id": "9859174", "ec_numbers": [ "1.8.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:15946682", "title": "Crystal structure of human dihydrolipoamide dehydrogenase: NAD+/NADH binding and the structural basis of disease-causing mutations", "journal": "J Mol Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15946682/" }, { "id": "PMID:28579060", "title": "Human dihydrolipoamide dehydrogenase (E3) deficiency: Novel insights into the structural basis and molecular pathomechanism", "journal": "Neurochem Int", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/28579060/" }, { "id": "PMID:26456061", "title": "Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components", "journal": "Free Radic Biol Med", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26456061/" }, { "id": "PMID:37701333", "title": "Biochemical characterization of patients with dihydrolipoamide dehydrogenase deficiency", "journal": "JIMD Rep", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37701333/" } ], "x": 7078.666666666667, "y": 1657.5833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861563", "label": "CTLH E3 ligase ubiquitinates LDHA", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9861563", "summary": "CTLH E3 ligase ubiquitinates LDHA is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects LDHA, Ub to PolyUb-LDHA in cytosol.", "equation": "LDHA + Ub → PolyUb-LDHA", "inputs": [ { "node_id": "R-HSA-70502", "reactome_id": "R-HSA-70502", "label": "LDHA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-113595", "reactome_id": "R-HSA-113595", "label": "Ub", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9861549", "reactome_id": "R-HSA-9861549", "label": "PolyUb-LDHA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "ubiquitin-protein transferase activity of CTLH E3 ligase [cytosol]", "activity_id": "9861628", "ec_numbers": [ "6.3.2.19" ], "accessions": [] } ], "citations": [ { "id": "PMID:31285494", "title": "The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation", "journal": "Sci Rep", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31285494/" }, { "id": "PMID:34383978", "title": "Proteomic analysis of ubiquitination substrates reveals a CTLH E3 ligase complex-dependent regulation of glycolysis", "journal": "FASEB J", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34383978/" }, { "id": "PMID:29911972", "title": "The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation", "journal": "Elife", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29911972/" } ], "x": 4451.333333333333, "y": 632.55, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9861595", "label": "Cytosolic H2O2 decarboxylates PYR to acetate", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9861595", "summary": "Cytosolic H2O2 decarboxylates PYR to acetate is a Reactome release 97 curated reaction event. The source record connects PYR, H2O2 to H2O, CO2, acetate in cytosol.", "equation": "PYR + H2O2 → H2O + CO2 + acetate", "inputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9717939", "reactome_id": "R-ALL-9717939", "label": "acetate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:30245009", "title": "Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals", "journal": "Cell", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30245009/" } ], "x": 4730, "y": 554.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861616", "label": "DLD dimer dehydrogenates dihydrolipoyl", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:15046" ], "source_url": "https://reactome.org/content/detail/R-HSA-9861616", "summary": "DLD dimer dehydrogenates dihydrolipoyl is a Reactome release 97 curated reaction event. The source record connects NAD+, LipoH2-K259-DLAT to H+, NADH, Lipo-K259-DLAT in mitochondrial matrix and cross-references RHEA:15046.", "equation": "NAD+ + LipoH2-K259-DLAT → H+ + NADH + Lipo-K259-DLAT", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9861664", "reactome_id": "R-HSA-9861664", "label": "LipoH2-K259-DLAT", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-69969", "reactome_id": "R-HSA-69969", "label": "Lipo-K259-DLAT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "dihydrolipoyl dehydrogenase (NADH) activity of PDH complex [mitochondrial matrix]", "activity_id": "9861687", "ec_numbers": [ "1.8.1.4" ], "accessions": [] } ], "citations": [ { "id": "PMID:15946682", "title": "Crystal structure of human dihydrolipoamide dehydrogenase: NAD+/NADH binding and the structural basis of disease-causing mutations", "journal": "J Mol Biol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15946682/" }, { "id": "PMID:28579060", "title": "Human dihydrolipoamide dehydrogenase (E3) deficiency: Novel insights into the structural basis and molecular pathomechanism", "journal": "Neurochem Int", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/28579060/" }, { "id": "PMID:26456061", "title": "Formation of reactive oxygen species by human and bacterial pyruvate and 2-oxoglutarate dehydrogenase multienzyme complexes reconstituted from recombinant components", "journal": "Free Radic Biol Med", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26456061/" }, { "id": "PMID:37701333", "title": "Biochemical characterization of patients with dihydrolipoamide dehydrogenase deficiency", "journal": "JIMD Rep", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37701333/" } ], "x": 4451.333333333333, "y": 476.95, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861626", "label": "SIRT4 cleaves lipoyl from DLAT", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:63641" ], "source_url": "https://reactome.org/content/detail/R-HSA-9861626", "summary": "SIRT4 cleaves lipoyl from DLAT is a Reactome release 97 curated reaction event. The source record connects H2O, Lipo-K259-DLAT, NAD+ to 2''-O-lipoyl-ADP-D-ribose, NAM, DLAT in mitochondrial matrix and cross-references RHEA:63641.", "equation": "H2O + Lipo-K259-DLAT + NAD+ → 2''-O-lipoyl-ADP-D-ribose + NAM + DLAT", "inputs": [ { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-69969", "reactome_id": "R-HSA-69969", "label": "Lipo-K259-DLAT", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9861625", "reactome_id": "R-ALL-9861625", "label": "2''-O-lipoyl-ADP-D-ribose", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:189572", "formula": "C23H33N5O15P2S2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5688274", "reactome_id": "R-ALL-5688274", "label": "NAM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-6792585", "reactome_id": "R-HSA-6792585", "label": "DLAT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "lipoamidase activity of SIRT4:Zn2+ [mitochondrial matrix]", "activity_id": "9861740", "ec_numbers": [ "3.5.1.138" ], "accessions": [] } ], "citations": [ { "id": "PMID:25525879", "title": "Sirtuin 4 is a lipoamidase regulating pyruvate dehydrogenase complex activity", "journal": "Cell", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25525879/" } ], "x": 4451.333333333333, "y": 438.04999999999995, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861631", "label": "ME1 tetramerizes", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9861631", "summary": "ME1 tetramerizes is a Reactome release 97 curated reaction event. The source record connects ME1, Mg2+/Mn2+ to ME1 tetramer in cytosol.", "equation": "4 ME1 + 4 Mg2+/Mn2+ → ME1 tetramer", "inputs": [ { "node_id": "R-HSA-400119", "reactome_id": "R-HSA-400119", "label": "ME1", "role": "input", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-1500648", "reactome_id": "R-ALL-1500648", "label": "Mg2+/Mn2+", "role": "input", "stoichiometry": 4, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9012061", "reactome_id": "R-HSA-9012061", "label": "ME1 tetramer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:31735643", "title": "Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis", "journal": "Mol Cell", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/31735643/" }, { "id": "PMID:7757881", "title": "Purification and properties of cytosolic and mitochondrial malic enzyme isolated from human brain", "journal": "Int J Biochem Cell Biol", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7757881/" } ], "x": 5008.666666666667, "y": 593.65, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861640", "label": "CTLH E3 ligase ubiquitinates PKM-1", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9861640", "summary": "CTLH E3 ligase ubiquitinates PKM-1 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Ub, PKM-1 to PolyUb-PKM-1 in cytosol.", "equation": "Ub + PKM-1 → PolyUb-PKM-1", "inputs": [ { "node_id": "R-HSA-113595", "reactome_id": "R-HSA-113595", "label": "Ub", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-71667", "reactome_id": "R-HSA-71667", "label": "PKM-1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9861692", "reactome_id": "R-HSA-9861692", "label": "PolyUb-PKM-1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "ubiquitin-protein transferase activity of CTLH E3 ligase [cytosol]", "activity_id": "9861628", "ec_numbers": [ "6.3.2.19" ], "accessions": [] } ], "citations": [ { "id": "PMID:31285494", "title": "The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation", "journal": "Sci Rep", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31285494/" }, { "id": "PMID:34383978", "title": "Proteomic analysis of ubiquitination substrates reveals a CTLH E3 ligase complex-dependent regulation of glycolysis", "journal": "FASEB J", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34383978/" }, { "id": "PMID:29911972", "title": "The multi-subunit GID/CTLH E3 ubiquitin ligase promotes cell proliferation and targets the transcription factor Hbp1 for degradation", "journal": "Elife", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29911972/" } ], "x": 4730, "y": 632.55, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9861642", "label": "NEK1 phosphorylates ME1", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:17990" ], "source_url": "https://reactome.org/content/detail/R-HSA-9861642", "summary": "NEK1 phosphorylates ME1 is a Reactome release 97 curated reaction event. The source record connects ME1, ATP to p-S336-ME1, ADP, H+ in cytosol and cross-references RHEA:17990.", "equation": "ME1 + ATP → p-S336-ME1 + ADP + H+", "inputs": [ { "node_id": "R-HSA-400119", "reactome_id": "R-HSA-400119", "label": "ME1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-9861606", "reactome_id": "R-HSA-9861606", "label": "p-S336-ME1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "protein serine/threonine kinase activity of NEK1:Mg2+ [cytosol]", "activity_id": "9861723", "ec_numbers": [ "2.7.11.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:31735643", "title": "Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis", "journal": "Mol Cell", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/31735643/" }, { "id": "PMID:37585529", "title": "Loss of function of the ALS-associated NEK1 kinase disrupts microtubule homeostasis and nuclear import", "journal": "Sci Adv", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37585529/" }, { "id": "PMID:33462636", "title": "NEK1 mutations and the risk of amyotrophic lateral sclerosis (ALS): a meta-analysis", "journal": "Neurol Sci", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33462636/" }, { "id": "PMID:22499340", "title": "NEK1 and DYNC2H1 are both involved in short rib polydactyly Majewski type but not in Beemer Langer cases", "journal": "J Med Genet", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22499340/" } ], "x": 4730, "y": 476.95, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861660", "label": "ME1 tetramer decarboxylates OA to PYR", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:15642" ], "source_url": "https://reactome.org/content/detail/R-HSA-9861660", "summary": "ME1 tetramer decarboxylates OA to PYR is a Reactome release 97 curated reaction event. The source record connects OA, H+ to PYR, CO2 in cytosol and cross-references RHEA:15642.", "equation": "OA + H+ → PYR + CO2", "inputs": [ { "node_id": "R-ALL-76213", "reactome_id": "R-ALL-76213", "label": "OA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16452", "formula": "C4H2O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxaloacetate decarboxylase activity of ME1 tetramer [cytosol]", "activity_id": "9861583", "ec_numbers": [ "4.1.1.112" ], "accessions": [] } ], "citations": [ { "id": "PMID:7757881", "title": "Purification and properties of cytosolic and mitochondrial malic enzyme isolated from human brain", "journal": "Int J Biochem Cell Biol", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7757881/" }, { "id": "PMID:1935931", "title": "Malic enzyme in human liver. Intracellular distribution, purification and properties of cytosolic isozyme", "journal": "Eur. J. Biochem.", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1935931/" }, { "id": "PMID:14596586", "title": "Structure and function of malic enzymes, a new class of oxidative decarboxylases", "journal": "Biochemistry", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14596586/" }, { "id": "PMID:24998673", "title": "Structural characteristics of the nonallosteric human cytosolic malic enzyme", "journal": "Biochim. Biophys. Acta", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24998673/" }, { "id": "PMID:23284632", "title": "Biophysical characterization of the dimer and tetramer interface interactions of the human cytosolic malic enzyme", "journal": "PLoS ONE", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23284632/" } ], "x": 4451.333333333333, "y": 399.15, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861667", "label": "DLAT trimer transfers acetyl to CoA", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:17019" ], "source_url": "https://reactome.org/content/detail/R-HSA-9861667", "summary": "DLAT trimer transfers acetyl to CoA is a Reactome release 97 curated reaction event. The source record connects CoA-SH, AcetyllipoH2-K132,K259-DLAT to Ac-CoA, LipoH2-K259-DLAT in mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:17019.", "equation": "CoA-SH + AcetyllipoH2-K132,K259-DLAT → Ac-CoA + LipoH2-K259-DLAT", "inputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9861594", "reactome_id": "R-HSA-9861594", "label": "AcetyllipoH2-K132,K259-DLAT", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113559", "reactome_id": "R-ALL-113559", "label": "Ac-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9861664", "reactome_id": "R-HSA-9861664", "label": "LipoH2-K259-DLAT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "dihydrolipoyllysine-residue acetyltransferase activity of PDH complex [mitochondrial matrix]", "activity_id": "9861662", "ec_numbers": [ "2.3.1.12" ], "accessions": [] } ], "citations": [ { "id": "PMID:9045657", "title": "Assembly and full functionality of recombinantly expressed dihydrolipoyl acetyltransferase component of the human pyruvate dehydrogenase complex", "journal": "J Biol Chem", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9045657/" }, { "id": "PMID:3174635", "title": "Primary structure of the human M2 mitochondrial autoantigen of primary biliary cirrhosis: dihydrolipoamide acetyltransferase", "journal": "Proc Natl Acad Sci U S A", "year": 1988, "url": "https://pubmed.ncbi.nlm.nih.gov/3174635/" }, { "id": "PMID:16049940", "title": "Clinical and genetic spectrum of pyruvate dehydrogenase deficiency: dihydrolipoamide acetyltransferase (E2) deficiency", "journal": "Ann Neurol", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/16049940/" } ], "x": 4730, "y": 515.85, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861725", "label": "PGAM5 dodecamer dephosphorylates p-S336-ME1", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "cytosol · mitochondrial outer membrane", "compartment_ids": [ "GO:0005829", "GO:0005741" ], "rhea_ids": [ "RHEA:20630" ], "source_url": "https://reactome.org/content/detail/R-HSA-9861725", "summary": "PGAM5 dodecamer dephosphorylates p-S336-ME1 is a Reactome release 97 curated reaction event. The source record connects H2O, p-S336-ME1 to Pi, ME1 in cytosol · mitochondrial outer membrane and cross-references RHEA:20630.", "equation": "H2O + p-S336-ME1 → Pi + ME1", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9861606", "reactome_id": "R-HSA-9861606", "label": "p-S336-ME1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29372", "reactome_id": "R-ALL-29372", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-400119", "reactome_id": "R-HSA-400119", "label": "ME1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "protein serine/threonine phosphatase activity of PGAM5 dodecamer [mitochondrial outer membrane]", "activity_id": "9861707", "ec_numbers": [ "3.1.3.16" ], "accessions": [] } ], "citations": [ { "id": "PMID:31735643", "title": "Dynamic Regulation of ME1 Phosphorylation and Acetylation Affects Lipid Metabolism and Colorectal Tumorigenesis", "journal": "Mol Cell", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/31735643/" }, { "id": "PMID:30705304", "title": "Functional role of PGAM5 multimeric assemblies and their polymerization into filaments", "journal": "Nat Commun", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30705304/" }, { "id": "PMID:18387606", "title": "PGAM5 tethers a ternary complex containing Keap1 and Nrf2 to mitochondria", "journal": "Exp Cell Res", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18387606/" } ], "x": 5008.666666666667, "y": 515.85, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861734", "label": "PDH E1 decarboxylates PYR, transferring acetyl to DLAT", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [ "RHEA:19190" ], "source_url": "https://reactome.org/content/detail/R-HSA-9861734", "summary": "PDH E1 decarboxylates PYR, transferring acetyl to DLAT is a Reactome release 97 curated reaction event. The source record connects PYR, Lipo-K259-DLAT, H+ to AcetyllipoH2-K132,K259-DLAT, CO2 in mitochondrial inner membrane · mitochondrial matrix and cross-references RHEA:19190.", "equation": "PYR + Lipo-K259-DLAT + H+ → AcetyllipoH2-K132,K259-DLAT + CO2", "inputs": [ { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-69969", "reactome_id": "R-HSA-69969", "label": "Lipo-K259-DLAT", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-9861594", "reactome_id": "R-HSA-9861594", "label": "AcetyllipoH2-K132,K259-DLAT", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "pyruvate dehydrogenase (acetyl-transferring) activity of PDH complex [mitochondrial matrix]", "activity_id": "450854", "ec_numbers": [ "1.2.4.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:12651851", "title": "Structural basis for flip-flop action of thiamin pyrophosphate-dependent enzymes revealed by human pyruvate dehydrogenase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12651851/" }, { "id": "PMID:21908116", "title": "Pyruvate dehydrogenase deficiency and epilepsy", "journal": "Brain Dev", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21908116/" } ], "x": 5008.666666666667, "y": 476.95, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9861745", "label": "Mitochondrial H2O2 decarboxylates PYR to acetate", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9861745", "summary": "Mitochondrial H2O2 decarboxylates PYR to acetate is a Reactome release 97 curated reaction event. The source record connects H2O2, PYR to CO2, H2O, CH3COO- in mitochondrial matrix.", "equation": "H2O2 + PYR → CO2 + H2O + CH3COO-", "inputs": [ { "node_id": "R-ALL-1614617", "reactome_id": "R-ALL-1614617", "label": "H2O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113557", "reactome_id": "R-ALL-113557", "label": "PYR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29376", "reactome_id": "R-ALL-29376", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113539", "reactome_id": "R-ALL-113539", "label": "CH3COO-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:30089", "formula": "C2H3O2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:30245009", "title": "Acetate Production from Glucose and Coupling to Mitochondrial Metabolism in Mammals", "journal": "Cell", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30245009/" } ], "x": 5008.666666666667, "y": 554.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9863531", "label": "GS-MCA spontaneously hydrolyzes", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "pyruvate" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9863531", "summary": "GS-MCA spontaneously hydrolyzes is a Reactome release 97 curated reaction event. The source record connects GS-MCA, H2O to GSH, glyoxylate, H+ in mitochondrial matrix.", "equation": "GS-MCA + H2O → GSH + glyoxylate + H+ + Cl-", "inputs": [ { "node_id": "R-ALL-9861569", "reactome_id": "R-ALL-9861569", "label": "GS-MCA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:229639", "formula": "C12H17ClN3O8S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-1655887", "reactome_id": "R-ALL-1655887", "label": "GSH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16856", "formula": "C10H17N3O6S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-904849", "reactome_id": "R-ALL-904849", "label": "glyoxylate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9861558", "reactome_id": "R-ALL-9861558", "label": "Cl-", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17996", "formula": "Cl", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:9815194", "title": "Glutathione transferase zeta-catalyzed biotransformation of dichloroacetic acid and other alpha-haloacids", "journal": "Chem Res Toxicol", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9815194/" } ], "x": 4451.333333333333, "y": 554.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9865309", "label": "MT-CO1 mRNA is translated", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865309", "summary": "MT-CO1 mRNA is translated is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MT-CO1 mRNA to nascent MT-CO1 in mitochondrial inner membrane · mitochondrial matrix.", "equation": "MT-CO1 mRNA → nascent MT-CO1", "inputs": [ { "node_id": "R-HSA-6793012", "reactome_id": "R-HSA-6793012", "label": "MT-CO1 mRNA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-164314", "reactome_id": "R-HSA-164314", "label": "nascent MT-CO1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:19503089", "title": "Mutation in TACO1, encoding a translational activator of COX I, results in cytochrome c oxidase deficiency and late-onset Leigh syndrome", "journal": "Nat Genet", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19503089/" }, { "id": "PMID:37247699", "title": "PET117 assembly factor stabilizes translation activator TACO1 thereby upregulates mitochondria-encoded cytochrome C oxidase 1 synthesis", "journal": "Free Radic Biol Med", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/37247699/" }, { "id": "PMID:27693358", "title": "Mitochondrial Protein Synthesis Adapts to Influx of Nuclear-Encoded Protein", "journal": "Cell", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/27693358/" }, { "id": "PMID:28199844", "title": "MR-1S Interacts with PET100 and PET117 in Module-Based Assembly of Human Cytochrome c Oxidase", "journal": "Cell Rep", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28199844/" }, { "id": "PMID:34969438", "title": "Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors", "journal": "Elife", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34969438/" } ], "x": 5008.666666666667, "y": 1092.1, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9865350", "label": "Nascent MT-CO1 binds to MITRAC, Mg2+, PE, CL", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865350", "summary": "Nascent MT-CO1 binds to MITRAC, Mg2+, PE, CL is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MITRAC, nascent MT-CO1, Mg2+ to MT-CO1:Mg2+:PE:CL:MITRAC in mitochondrial inner membrane.", "equation": "MITRAC + nascent MT-CO1 + Mg2+ + PE + cardiolipin → MT-CO1:Mg2+:PE:CL:MITRAC", "inputs": [ { "node_id": "R-HSA-9865338", "reactome_id": "R-HSA-9865338", "label": "MITRAC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-164314", "reactome_id": "R-HSA-164314", "label": "nascent MT-CO1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-109496", "reactome_id": "R-ALL-109496", "label": "Mg2+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18420", "formula": "Mg", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524083", "reactome_id": "R-ALL-1524083", "label": "PE", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57613", "formula": "C7H12NO8PR2", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-1524072", "reactome_id": "R-ALL-1524072", "label": "cardiolipin", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28494", "formula": "C13H18O17P2R4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9865433", "reactome_id": "R-HSA-9865433", "label": "MT-CO1:Mg2+:PE:CL:MITRAC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:23260140", "title": "MITRAC links mitochondrial protein translocation to respiratory-chain assembly and translational regulation", "journal": "Cell", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23260140/" }, { "id": "PMID:26321642", "title": "MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly", "journal": "Cell Rep", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26321642/" }, { "id": "PMID:28082314", "title": "A CMC1 -knockout reveals translation-independent control of human mitochondrial complex IV biogenesis", "journal": "EMBO Rep", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28082314/" }, { "id": "PMID:9843204", "title": "SURF1, encoding a factor involved in the biogenesis of cytochrome c oxidase, is mutated in Leigh syndrome", "journal": "Nat Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9843204/" } ], "x": 4451.333333333333, "y": 1040.2333333333333, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9865412", "label": "TIMM21 carries COX4, COX5A, COX6C to MT-CO1:MITRAC", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005743", "GO:0005758" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865412", "summary": "TIMM21 carries COX4, COX5A, COX6C to MT-CO1:MITRAC is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects TIMM21:COX4:COX5A:COX6C, MT-CO1:Mg2+:PE:CL:MITRAC to MT-CO1:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C in mitochondrial inner membrane · mitochondrial intermembrane space.", "equation": "TIMM21:COX4:COX5A:COX6C + MT-CO1:Mg2+:PE:CL:MITRAC → MT-CO1:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C", "inputs": [ { "node_id": "R-HSA-9865418", "reactome_id": "R-HSA-9865418", "label": "TIMM21:COX4:COX5A:COX6C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9865433", "reactome_id": "R-HSA-9865433", "label": "MT-CO1:Mg2+:PE:CL:MITRAC", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9865410", "reactome_id": "R-HSA-9865410", "label": "MT-CO1:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:28082314", "title": "A CMC1 -knockout reveals translation-independent control of human mitochondrial complex IV biogenesis", "journal": "EMBO Rep", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28082314/" }, { "id": "PMID:23260140", "title": "MITRAC links mitochondrial protein translocation to respiratory-chain assembly and translational regulation", "journal": "Cell", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/23260140/" }, { "id": "PMID:26321642", "title": "MITRAC7 Acts as a COX1-Specific Chaperone and Reveals a Checkpoint during Cytochrome c Oxidase Assembly", "journal": "Cell Rep", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26321642/" } ], "x": 5008.666666666667, "y": 988.3666666666667, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9865449", "label": "Metallochaperone inserts Cu2+ into MT-CO1", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865449", "summary": "Metallochaperone inserts Cu2+ into MT-CO1 is a Reactome release 97 curated reaction event. The source record connects MT-CO1:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C, COX17:Cu to MT-CO1:Cu2+:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C, COX17 in mitochondrial inner membrane.", "equation": "MT-CO1:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C + COX17:Cu → MT-CO1:Cu2+:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C + COX17", "inputs": [ { "node_id": "R-HSA-9865410", "reactome_id": "R-HSA-9865410", "label": "MT-CO1:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9865624", "reactome_id": "R-HSA-9865624", "label": "COX17:Cu", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9865438", "reactome_id": "R-HSA-9865438", "label": "MT-CO1:Cu2+:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-1307788", "reactome_id": "R-HSA-1307788", "label": "COX17", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "copper chaperone activity of metallochaperone complex [mitochondrial inner membrane]", "activity_id": "9865627", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:35750769", "title": "Coordination of metal center biogenesis in human cytochrome c oxidase", "journal": "Nat Commun", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35750769/" }, { "id": "PMID:23345593", "title": "COX19 mediates the transduction of a mitochondrial redox signal from SCO1 that regulates ATP7A-mediated cellular copper efflux", "journal": "Mol Biol Cell", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23345593/" }, { "id": "PMID:25926683", "title": "Redox-regulated dynamic interplay between Cox19 and the copper-binding protein Cox11 in the intermembrane space of mitochondria facilitates biogenesis of cytochrome c oxidase", "journal": "Mol Biol Cell", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25926683/" }, { "id": "PMID:34504870", "title": "Role of Copper on Mitochondrial Function and Metabolism", "journal": "Front Mol Biosci", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34504870/" } ], "x": 4730, "y": 858.7, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9865569", "label": "MT-CO2 mRNA is translated", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865569", "summary": "MT-CO2 mRNA is translated is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MT-CO2 mRNA to nascent MT-CO2 in mitochondrial inner membrane · mitochondrial matrix.", "equation": "MT-CO2 mRNA → nascent MT-CO2", "inputs": [ { "node_id": "R-HSA-6792984", "reactome_id": "R-HSA-6792984", "label": "MT-CO2 mRNA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9902095", "reactome_id": "R-HSA-9902095", "label": "nascent MT-CO2", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:28330871", "title": "Human mitochondrial cytochrome c oxidase assembly factor COX18 acts transiently as a membrane insertase within the subunit 2 maturation module", "journal": "J Biol Chem", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28330871/" } ], "x": 4451.333333333333, "y": 1118.0333333333333, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9865579", "label": "MT-CO1 and MT-CO2 complexes associate, installing heme moieties", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865579", "summary": "MT-CO1 and MT-CO2 complexes associate, installing heme moieties is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects COX7B, COX7C, MT-CO1:Cu2+:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C to holo-MT-CO1,2 complex, TIMM21 in mitochondrial inner membrane.", "equation": "COX7B + COX7C + MT-CO1:Cu2+:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C + MT-CO2:Mg2+:CuA + 3 heme A + COX8A,C → holo-MT-CO1,2 complex + TIMM21", "inputs": [ { "node_id": "R-HSA-164309", "reactome_id": "R-HSA-164309", "label": "COX7B", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-164306", "reactome_id": "R-HSA-164306", "label": "COX7C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9865438", "reactome_id": "R-HSA-9865438", "label": "MT-CO1:Cu2+:Mg2+:PE:CL:MITRAC:TIMM21:COX4:COX5A:COX6C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9865631", "reactome_id": "R-HSA-9865631", "label": "MT-CO2:Mg2+:CuA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9864492", "reactome_id": "R-ALL-9864492", "label": "heme A", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:61715", "formula": "C49H54FeN4O6", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-5336142", "reactome_id": "R-HSA-5336142", "label": "COX8A,C", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9865576", "reactome_id": "R-HSA-9865576", "label": "holo-MT-CO1,2 complex", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9865416", "reactome_id": "R-HSA-9865416", "label": "TIMM21", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "copper chaperone activity of metallochaperone complex [mitochondrial inner membrane]", "activity_id": "9865627", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:33129245", "title": "Role of cytochrome c oxidase nuclear-encoded subunits in health and disease", "journal": "Physiol Res", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33129245/" }, { "id": "PMID:25959673", "title": "Cooperation between COA6 and SCO2 in COX2 maturation during cytochrome c oxidase assembly links two mitochondrial cardiomyopathies", "journal": "Cell Metab.", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25959673/" }, { "id": "PMID:9843204", "title": "SURF1, encoding a factor involved in the biogenesis of cytochrome c oxidase, is mutated in Leigh syndrome", "journal": "Nat Genet", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9843204/" }, { "id": "PMID:15764605", "title": "Assembly of cytochrome-c oxidase in the absence of assembly protein Surf1p leads to loss of the active site heme", "journal": "J Biol Chem", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15764605/" }, { "id": "PMID:19625251", "title": "Surf1, associated with Leigh syndrome in humans, is a heme-binding protein in bacterial oxidase biogenesis", "journal": "J Biol Chem", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19625251/" }, { "id": "PMID:34428995", "title": "Biosynthesis and trafficking of heme o and heme a : new structural insights and their implications for reaction mechanisms and prenylated heme transfer", "journal": "Crit Rev Biochem Mol Biol", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34428995/" } ], "x": 5008.666666666667, "y": 1014.3, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9865630", "label": "Metallochaperone inserts 2Cu2+ into MT-CO2", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865630", "summary": "Metallochaperone inserts 2Cu2+ into MT-CO2 is a Reactome release 97 curated reaction event. The source record connects COX17:Cu, MT-CO2:COX20:TMEM177 to COX17, MT-CO2:Mg2+:CuA, COX20:TMEM177 in mitochondrial inner membrane.", "equation": "2 COX17:Cu + MT-CO2:COX20:TMEM177 → 2 COX17 + MT-CO2:Mg2+:CuA + COX20:TMEM177", "inputs": [ { "node_id": "R-HSA-9865624", "reactome_id": "R-HSA-9865624", "label": "COX17:Cu", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9865557", "reactome_id": "R-HSA-9865557", "label": "MT-CO2:COX20:TMEM177", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1307788", "reactome_id": "R-HSA-1307788", "label": "COX17", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9865631", "reactome_id": "R-HSA-9865631", "label": "MT-CO2:Mg2+:CuA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9865587", "reactome_id": "R-HSA-9865587", "label": "COX20:TMEM177", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "copper chaperone activity of metallochaperone complex [mitochondrial inner membrane]", "activity_id": "9865627", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:35750769", "title": "Coordination of metal center biogenesis in human cytochrome c oxidase", "journal": "Nat Commun", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35750769/" }, { "id": "PMID:25926683", "title": "Redox-regulated dynamic interplay between Cox19 and the copper-binding protein Cox11 in the intermembrane space of mitochondria facilitates biogenesis of cytochrome c oxidase", "journal": "Mol Biol Cell", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25926683/" }, { "id": "PMID:34504870", "title": "Role of Copper on Mitochondrial Function and Metabolism", "journal": "Front Mol Biosci", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34504870/" } ], "x": 5008.666666666667, "y": 858.7, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9865663", "label": "MT-CO3, COX6A,B,7A and NDUFA4 bind to holo-MT-CO1,2 complex", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865663", "summary": "MT-CO3, COX6A,B,7A and NDUFA4 bind to holo-MT-CO1,2 complex is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MT-CO3:PE:CL, NDUFA4:CL, COX7A1,2,2L to Cytochrome c oxidase, MITRAC in mitochondrial inner membrane.", "equation": "MT-CO3:PE:CL + NDUFA4:CL + COX7A1,2,2L + COX6A:PE + COX6B1,2 + holo-MT-CO1,2 complex → Cytochrome c oxidase + MITRAC", "inputs": [ { "node_id": "R-HSA-9864495", "reactome_id": "R-HSA-9864495", "label": "MT-CO3:PE:CL", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9864472", "reactome_id": "R-HSA-9864472", "label": "NDUFA4:CL", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-5336144", "reactome_id": "R-HSA-5336144", "label": "COX7A1,2,2L", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-9864460", "reactome_id": "R-HSA-9864460", "label": "COX6A:PE", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-5336172", "reactome_id": "R-HSA-5336172", "label": "COX6B1,2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-HSA-9865576", "reactome_id": "R-HSA-9865576", "label": "holo-MT-CO1,2 complex", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-164316", "reactome_id": "R-HSA-164316", "label": "Cytochrome c oxidase", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9865338", "reactome_id": "R-HSA-9865338", "label": "MITRAC", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:22342701", "title": "Rcf1 mediates cytochrome oxidase assembly and respirasome formation, revealing heterogeneity of the enzyme complex", "journal": "Cell Metab.", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22342701/" }, { "id": "PMID:32375044", "title": "Distinct Roles of Mitochondrial HIGD1A and HIGD2A in Respiratory Complex and Supercomplex Biogenesis", "journal": "Cell Rep", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32375044/" }, { "id": "PMID:33291261", "title": "HIGD-Driven Regulation of Cytochrome c Oxidase Biogenesis and Function", "journal": "Cells", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33291261/" }, { "id": "PMID:32317297", "title": "HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV", "journal": "Mol Cell Proteomics", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32317297/" }, { "id": "PMID:28870773", "title": "Mitochondrial cytochrome c oxidase biogenesis: Recent developments", "journal": "Semin Cell Dev Biol", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/28870773/" }, { "id": "PMID:31536960", "title": "Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis", "journal": "iScience", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31536960/" } ], "x": 4730, "y": 1118.0333333333333, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9865893", "label": "MT-CYB is translated", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9865893", "summary": "MT-CYB is translated is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MT-CYB mRNA, UQCC1:UQCC2:UQCC5:UQCC6 to Intermediate 0 in mitochondrial inner membrane · mitochondrial matrix.", "equation": "MT-CYB mRNA + UQCC1:UQCC2:UQCC5:UQCC6 → Intermediate 0", "inputs": [ { "node_id": "R-HSA-9865896", "reactome_id": "R-HSA-9865896", "label": "MT-CYB mRNA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9865909", "reactome_id": "R-HSA-9865909", "label": "UQCC1:UQCC2:UQCC5:UQCC6", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9865914", "reactome_id": "R-HSA-9865914", "label": "Intermediate 0", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein folding chaperone activity of UQCC1:UQCC2:UQCC5:UQCC6 [mitochondrial inner membrane]", "activity_id": "9865905", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:29733856", "title": "Biogenesis of the bc 1 Complex of the Mitochondrial Respiratory Chain", "journal": "J Mol Biol", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29733856/" }, { "id": "PMID:36142449", "title": "Assembly of the Multi-Subunit Cytochrome bc 1 Complex in the Yeast Saccharomyces cerevisiae", "journal": "Int J Mol Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36142449/" }, { "id": "PMID:34969438", "title": "Defining the interactome of the human mitochondrial ribosome identifies SMIM4 and TMEM223 as respiratory chain assembly factors", "journal": "Elife", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/34969438/" }, { "id": "PMID:24385928", "title": "Mutations in the UQCC1-interacting protein, UQCC2, cause human complex III deficiency associated with perturbed cytochrome b protein expression", "journal": "PLoS Genet", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24385928/" } ], "x": 4730, "y": 1040.2333333333333, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9866061", "label": "Intermediate 0 binds heme and UQCC3", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9866061", "summary": "Intermediate 0 binds heme and UQCC3 is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects UQCC3, heme b, Intermediate 0 to Intermediate I in mitochondrial inner membrane.", "equation": "UQCC3 + heme b + Intermediate 0 → Intermediate I", "inputs": [ { "node_id": "R-HSA-9866062", "reactome_id": "R-HSA-9866062", "label": "UQCC3", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9853511", "reactome_id": "R-ALL-9853511", "label": "heme b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:26355", "formula": "C34H32FeN4O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9865914", "reactome_id": "R-HSA-9865914", "label": "Intermediate 0", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9866058", "reactome_id": "R-HSA-9866058", "label": "Intermediate I", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:25605331", "title": "C11orf83, a mitochondrial cardiolipin-binding protein involved in bc1 complex assembly and supercomplex stabilization", "journal": "Mol Cell Biol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/25605331/" }, { "id": "PMID:29733856", "title": "Biogenesis of the bc 1 Complex of the Mitochondrial Respiratory Chain", "journal": "J Mol Biol", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29733856/" }, { "id": "PMID:36142449", "title": "Assembly of the Multi-Subunit Cytochrome bc 1 Complex in the Yeast Saccharomyces cerevisiae", "journal": "Int J Mol Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36142449/" }, { "id": "PMID:25008109", "title": "A mutation in the human CBP4 ortholog UQCC3 impairs complex III assembly, activity and cytochrome b stability", "journal": "Hum Mol Genet", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/25008109/" } ], "x": 5008.666666666667, "y": 1066.1666666666667, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9866104", "label": "Intermediate I binds second heme, UQCRB, UQCRQ", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9866104", "summary": "Intermediate I binds second heme, UQCRB, UQCRQ is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects UQCRB, UQCRQ, Intermediate I to Intermediate II, UQCC1:UQCC2:UQCC5:UQCC6 in mitochondrial inner membrane.", "equation": "UQCRB + UQCRQ + Intermediate I + heme b → Intermediate II + UQCC1:UQCC2:UQCC5:UQCC6", "inputs": [ { "node_id": "R-HSA-164287", "reactome_id": "R-HSA-164287", "label": "UQCRB", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-164283", "reactome_id": "R-HSA-164283", "label": "UQCRQ", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9866058", "reactome_id": "R-HSA-9866058", "label": "Intermediate I", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9853511", "reactome_id": "R-ALL-9853511", "label": "heme b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:26355", "formula": "C34H32FeN4O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9866106", "reactome_id": "R-HSA-9866106", "label": "Intermediate II", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9865909", "reactome_id": "R-HSA-9865909", "label": "UQCC1:UQCC2:UQCC5:UQCC6", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:29733856", "title": "Biogenesis of the bc 1 Complex of the Mitochondrial Respiratory Chain", "journal": "J Mol Biol", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29733856/" }, { "id": "PMID:36142449", "title": "Assembly of the Multi-Subunit Cytochrome bc 1 Complex in the Yeast Saccharomyces cerevisiae", "journal": "Int J Mol Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36142449/" }, { "id": "PMID:28844695", "title": "Architecture of Human Mitochondrial Respiratory Megacomplex I 2 III 2 IV 2", "journal": "Cell", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28844695/" } ], "x": 4451.333333333333, "y": 1092.1, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9866132", "label": "Intermediate II binds CYC1, UQCRC1, UQCRC2, UQCRH", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9866132", "summary": "Intermediate II binds CYC1, UQCRC1, UQCRC2, UQCRH is a Reactome release 97 curated reaction event. The source record connects CYC1:heme c, Intermediate II, UQCRC1 to Late core complex monomer, UQCC3 in mitochondrial inner membrane.", "equation": "CYC1:heme c + Intermediate II + UQCRC1 + UQCRC2 + UQCRH, (UQCRHL) → Late core complex monomer + UQCC3", "inputs": [ { "node_id": "R-HSA-164584", "reactome_id": "R-HSA-164584", "label": "CYC1:heme c", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9866106", "reactome_id": "R-HSA-9866106", "label": "Intermediate II", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-164284", "reactome_id": "R-HSA-164284", "label": "UQCRC1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-164280", "reactome_id": "R-HSA-164280", "label": "UQCRC2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9932280", "reactome_id": "R-HSA-9932280", "label": "UQCRH, (UQCRHL)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "CandidateSet", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9866126", "reactome_id": "R-HSA-9866126", "label": "Late core complex monomer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9866062", "reactome_id": "R-HSA-9866062", "label": "UQCC3", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "protein binding activity of Intermediate II [mitochondrial inner membrane]", "activity_id": "9907159", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:29733856", "title": "Biogenesis of the bc 1 Complex of the Mitochondrial Respiratory Chain", "journal": "J Mol Biol", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29733856/" }, { "id": "PMID:36142449", "title": "Assembly of the Multi-Subunit Cytochrome bc 1 Complex in the Yeast Saccharomyces cerevisiae", "journal": "Int J Mol Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36142449/" }, { "id": "PMID:28844695", "title": "Architecture of Human Mitochondrial Respiratory Megacomplex I 2 III 2 IV 2", "journal": "Cell", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28844695/" }, { "id": "PMID:32929120", "title": "UQCRH downregulation promotes Warburg effect in renal cell carcinoma cells", "journal": "Sci Rep", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32929120/" } ], "x": 4451.333333333333, "y": 884.6333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9866162", "label": "Late core complex dimerizes", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9866162", "summary": "Late core complex dimerizes is a Reactome release 97 curated reaction event. The source record connects Late core complex monomer to Late core complex dimer in mitochondrial inner membrane.", "equation": "2 Late core complex monomer → Late core complex dimer", "inputs": [ { "node_id": "R-HSA-9866126", "reactome_id": "R-HSA-9866126", "label": "Late core complex monomer", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9866174", "reactome_id": "R-HSA-9866174", "label": "Late core complex dimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:29733856", "title": "Biogenesis of the bc 1 Complex of the Mitochondrial Respiratory Chain", "journal": "J Mol Biol", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29733856/" }, { "id": "PMID:36142449", "title": "Assembly of the Multi-Subunit Cytochrome bc 1 Complex in the Yeast Saccharomyces cerevisiae", "journal": "Int J Mol Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36142449/" }, { "id": "PMID:28844695", "title": "Architecture of Human Mitochondrial Respiratory Megacomplex I 2 III 2 IV 2", "journal": "Cell", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28844695/" } ], "x": 4451.333333333333, "y": 1014.3, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9866164", "label": "Late core complex dimer binds UQCR10, UQCR11, UQCRFS1.", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9866164", "summary": "Late core complex dimer binds UQCR10, UQCR11, UQCRFS1. is a Reactome release 97 curated reaction event. The source record connects UQCR10, UQCR11, Late core complex dimer to Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(1-274) in mitochondrial inner membrane.", "equation": "2 UQCR10 + 2 UQCR11 + Late core complex dimer + 2 holo-UQCRFS1(1-274) → Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(1-274)", "inputs": [ { "node_id": "R-HSA-164282", "reactome_id": "R-HSA-164282", "label": "UQCR10", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-164285", "reactome_id": "R-HSA-164285", "label": "UQCR11", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9866174", "reactome_id": "R-HSA-9866174", "label": "Late core complex dimer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-164304", "reactome_id": "R-HSA-164304", "label": "holo-UQCRFS1(1-274)", "role": "input", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9906021", "reactome_id": "R-HSA-9906021", "label": "Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(1-274)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:29733856", "title": "Biogenesis of the bc 1 Complex of the Mitochondrial Respiratory Chain", "journal": "J Mol Biol", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29733856/" }, { "id": "PMID:36142449", "title": "Assembly of the Multi-Subunit Cytochrome bc 1 Complex in the Yeast Saccharomyces cerevisiae", "journal": "Int J Mol Sci", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/36142449/" }, { "id": "PMID:28844695", "title": "Architecture of Human Mitochondrial Respiratory Megacomplex I 2 III 2 IV 2", "journal": "Cell", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28844695/" } ], "x": 4730, "y": 1014.3, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9866217", "label": "HCCS inserts heme into cytochrome c", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [ "RHEA:22650" ], "source_url": "https://reactome.org/content/detail/R-HSA-9866217", "summary": "HCCS inserts heme into cytochrome c is a Reactome release 97 curated reaction event. The source record connects heme b, apo-CYCS to Cytochrome c (reduced) in mitochondrial inner membrane and cross-references RHEA:22650.", "equation": "heme b + apo-CYCS → Cytochrome c (reduced)", "inputs": [ { "node_id": "R-ALL-9853511", "reactome_id": "R-ALL-9853511", "label": "heme b", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:26355", "formula": "C34H32FeN4O4", "compartment": "mitochondrial inner membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-352608", "reactome_id": "R-HSA-352608", "label": "apo-CYCS", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-352609", "reactome_id": "R-HSA-352609", "label": "Cytochrome c (reduced)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "holocytochrome-c synthase activity of HCCS [mitochondrial inner membrane]", "activity_id": "9866232", "ec_numbers": [ "4.4.1.17" ], "accessions": [ "P53701" ] } ], "citations": [ { "id": "PMID:23150584", "title": "Human mitochondrial holocytochrome c synthase's heme binding, maturation determinants, and complex formation with cytochrome c", "journal": "Proc Natl Acad Sci U S A", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23150584/" }, { "id": "PMID:17033964", "title": "Mutations of the mitochondrial holocytochrome c-type synthase in X-linked dominant microphthalmia with linear skin defects syndrome", "journal": "Am J Hum Genet", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/17033964/" } ], "x": 4730, "y": 832.7666666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9866253", "label": "apo-UQCRFS1 binds LYRM7", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9866253", "summary": "apo-UQCRFS1 binds LYRM7 is a Reactome release 97 curated reaction event. The source record connects LYRM7, UQCRFS1(1-274) to UQCRFS1(1-274):LYRM7 in mitochondrial matrix.", "equation": "LYRM7 + UQCRFS1(1-274) → UQCRFS1(1-274):LYRM7", "inputs": [ { "node_id": "R-HSA-9866252", "reactome_id": "R-HSA-9866252", "label": "LYRM7", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-9906006", "reactome_id": "R-HSA-9906006", "label": "UQCRFS1(1-274)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9866263", "reactome_id": "R-HSA-9866263", "label": "UQCRFS1(1-274):LYRM7", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "protein folding chaperone activity of LYRM7 [mitochondrial matrix]", "activity_id": "9906879", "ec_numbers": null, "accessions": [ "Q5U5X0" ] } ], "citations": [ { "id": "PMID:23168492", "title": "LYRM7/MZM1L is a UQCRFS1 chaperone involved in the last steps of mitochondrial Complex III assembly in human cells", "journal": "Biochim Biophys Acta", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23168492/" }, { "id": "PMID:29243944", "title": "Mitochondrial complex III Rieske Fe-S protein processing and assembly", "journal": "Cell Cycle", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29243944/" }, { "id": "PMID:36757047", "title": "LYRM7-associated mitochondrial complex III deficiency with non-cavitating leukoencephalopathy and stroke-like episodes", "journal": "Am J Med Genet A", "year": 2023, "url": "https://pubmed.ncbi.nlm.nih.gov/36757047/" }, { "id": "PMID:28380382", "title": "A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III", "journal": "Cell Metab", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28380382/" } ], "x": 4730, "y": 884.6333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9866272", "label": "2Fe-2S is inserted in UQCRFS1", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9866272", "summary": "2Fe-2S is inserted in UQCRFS1 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster, UQCRFS1(1-274):LYRM7 to FXN:ISD11:NFS1:ISCU, holo-UQCRFS1(1-274), LYRM7 in mitochondrial inner membrane · mitochondrial matrix.", "equation": "FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster + UQCRFS1(1-274):LYRM7 → FXN:ISD11:NFS1:ISCU + holo-UQCRFS1(1-274) + LYRM7", "inputs": [ { "node_id": "R-HSA-1362398", "reactome_id": "R-HSA-1362398", "label": "FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9866263", "reactome_id": "R-HSA-9866263", "label": "UQCRFS1(1-274):LYRM7", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-1362404", "reactome_id": "R-HSA-1362404", "label": "FXN:ISD11:NFS1:ISCU", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-164304", "reactome_id": "R-HSA-164304", "label": "holo-UQCRFS1(1-274)", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9866252", "reactome_id": "R-HSA-9866252", "label": "LYRM7", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "iron chaperone activity of FXN:NFS1:ISD11:ISCU:2Fe-2S Cluster [mitochondrial matrix]", "activity_id": "9866276", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:28380382", "title": "A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III", "journal": "Cell Metab", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28380382/" }, { "id": "PMID:29243944", "title": "Mitochondrial complex III Rieske Fe-S protein processing and assembly", "journal": "Cell Cycle", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29243944/" } ], "x": 5008.666666666667, "y": 884.6333333333333, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9902096", "label": "COX18 inserts nascent MT-CO2 in COX20:TMEM77", "schema_class": "Reaction", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9902096", "summary": "COX18 inserts nascent MT-CO2 in COX20:TMEM77 is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects Mg2+, COX20:TMEM177, nascent MT-CO2 to MT-CO2:COX20:TMEM177 in mitochondrial inner membrane · mitochondrial matrix.", "equation": "Mg2+ + COX20:TMEM177 + nascent MT-CO2 → MT-CO2:COX20:TMEM177", "inputs": [ { "node_id": "R-ALL-109496", "reactome_id": "R-ALL-109496", "label": "Mg2+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:18420", "formula": "Mg", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-9865587", "reactome_id": "R-HSA-9865587", "label": "COX20:TMEM177", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9902095", "reactome_id": "R-HSA-9902095", "label": "nascent MT-CO2", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9865557", "reactome_id": "R-HSA-9865557", "label": "MT-CO2:COX20:TMEM177", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "membrane insertase activity of COX18 [mitochondrial inner membrane]", "activity_id": "9865559", "ec_numbers": null, "accessions": [ "Q8N8Q8" ] } ], "citations": [ { "id": "PMID:28330871", "title": "Human mitochondrial cytochrome c oxidase assembly factor COX18 acts transiently as a membrane insertase within the subunit 2 maturation module", "journal": "J Biol Chem", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28330871/" }, { "id": "PMID:23125284", "title": "A mutation in the FAM36A gene, the human ortholog of COX20, impairs cytochrome c oxidase assembly and is associated with ataxia and muscle hypotonia", "journal": "Hum Mol Genet", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23125284/" }, { "id": "PMID:24403053", "title": "Human COX20 cooperates with SCO1 and SCO2 to mature COX2 and promote the assembly of cytochrome c oxidase", "journal": "Hum Mol Genet", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24403053/" }, { "id": "PMID:29154948", "title": "The mitochondrial TMEM177 associates with COX20 during COX2 biogenesis", "journal": "Biochim Biophys Acta Mol Cell Res", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29154948/" } ], "x": 4451.333333333333, "y": 858.7, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9906017", "label": "Unknown peptidase cleaves UQCRFS1 subunit", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9906017", "summary": "Unknown peptidase cleaves UQCRFS1 subunit is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(1-274) to Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(79-274):Fragments in mitochondrial inner membrane.", "equation": "Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(1-274) → Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(79-274):Fragments", "inputs": [ { "node_id": "R-HSA-9906021", "reactome_id": "R-HSA-9906021", "label": "Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(1-274)", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9906014", "reactome_id": "R-HSA-9906014", "label": "Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(79-274):Fragments", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "endopeptidase activity of Unidentified protease [mitochondrial inner membrane]", "activity_id": "9906015", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:28673544", "title": "TTC19 Plays a Husbandry Role on UQCRFS1 Turnover in the Biogenesis of Mitochondrial Respiratory Complex III", "journal": "Mol Cell", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28673544/" }, { "id": "PMID:29243944", "title": "Mitochondrial complex III Rieske Fe-S protein processing and assembly", "journal": "Cell Cycle", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29243944/" } ], "x": 5008.666666666667, "y": 1040.2333333333333, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9906042", "label": "TTC19 clears UQCRFS1 fragments from Complex III", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9906042", "summary": "TTC19 clears UQCRFS1 fragments from Complex III is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(79-274):Fragments to Cytochrome c reductase, Unspecified N-terminal UQCRFS1 fragments in mitochondrial inner membrane.", "equation": "Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(79-274):Fragments → Cytochrome c reductase + 2 Unspecified N-terminal UQCRFS1 fragments", "inputs": [ { "node_id": "R-HSA-9906014", "reactome_id": "R-HSA-9906014", "label": "Dimer of Late core complex:UQCR10:UQCR11:holo-UQCRFS1(79-274):Fragments", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9866171", "reactome_id": "R-HSA-9866171", "label": "Cytochrome c reductase", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-9906010", "reactome_id": "R-ALL-9906010", "label": "Unspecified N-terminal UQCRFS1 fragments", "role": "output", "stoichiometry": 2, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "OtherEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:28673544", "title": "TTC19 Plays a Husbandry Role on UQCRFS1 Turnover in the Biogenesis of Mitochondrial Respiratory Complex III", "journal": "Mol Cell", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28673544/" }, { "id": "PMID:29243944", "title": "Mitochondrial complex III Rieske Fe-S protein processing and assembly", "journal": "Cell Cycle", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/29243944/" }, { "id": "PMID:21278747", "title": "Mutations in TTC19 cause mitochondrial complex III deficiency and neurological impairment in humans and flies", "journal": "Nat Genet", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21278747/" }, { "id": "PMID:37927170", "title": "A TTC19 mutation associated with progressive movement disorders and peripheral neuropathy: Case report and systematic review", "journal": "CNS Neurosci Ther", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/37927170/" } ], "x": 5008.666666666667, "y": 1118.0333333333333, "lod": "pathway", "collapsed": true, "transport": false }, { "id": "R-HSA-9906955", "label": "MT-ND4 is translated", "schema_class": "BlackBoxEvent", "domain_id": "central-energy", "pathway_ids": [ "electron-transport" ], "compartment": "mitochondrial inner membrane · mitochondrial matrix", "compartment_ids": [ "GO:0005743", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9906955", "summary": "MT-ND4 is translated is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects MT-ND4 mRNA to MT-ND4 in mitochondrial inner membrane · mitochondrial matrix.", "equation": "MT-ND4 mRNA → MT-ND4", "inputs": [ { "node_id": "R-HSA-6793019", "reactome_id": "R-HSA-6793019", "label": "MT-ND4 mRNA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-60561", "reactome_id": "R-HSA-60561", "label": "MT-ND4", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial inner membrane", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "membrane insertase activity of OXA1L:TMEM126A [mitochondrial inner membrane]", "activity_id": "9906939", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:17936786", "title": "Knockdown of human Oxa1l impairs the biogenesis of F1Fo-ATP synthase and NADH:ubiquinone oxidoreductase", "journal": "J Mol Biol", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17936786/" }, { "id": "PMID:38199007", "title": "Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria", "journal": "Mol Cell", "year": 2024, "url": "https://pubmed.ncbi.nlm.nih.gov/38199007/" }, { "id": "PMID:33882309", "title": "NDUFS3 depletion permits complex I maturation and reveals TMEM126A/OPA7 as an assembly factor binding the ND4-module intermediate", "journal": "Cell Rep", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33882309/" }, { "id": "PMID:33879611", "title": "Optic atrophy-associated TMEM126A is an assembly factor for the ND4-module of mitochondrial complex I", "journal": "Proc Natl Acad Sci U S A", "year": 2021, "url": "https://pubmed.ncbi.nlm.nih.gov/33879611/" } ], "x": 4451.333333333333, "y": 1066.1666666666667, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9911362", "label": "CPT1B transfers PALM to CAR", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "cytosol · mitochondrial outer membrane · mitochondrial intermembrane space", "compartment_ids": [ "GO:0005829", "GO:0005741", "GO:0005758" ], "rhea_ids": [ "RHEA:12661", "RHEA:12662" ], "source_url": "https://reactome.org/content/detail/R-HSA-9911362", "summary": "CPT1B transfers PALM to CAR is a Reactome release 97 curated reaction event. The source record connects CAR, PALM-CoA to CoA-SH, L-PCAR in cytosol · mitochondrial outer membrane · mitochondrial intermembrane space and cross-references RHEA:12661, RHEA:12662.", "equation": "CAR + PALM-CoA → CoA-SH + L-PCAR", "inputs": [ { "node_id": "R-ALL-549207", "reactome_id": "R-ALL-549207", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-200993", "reactome_id": "R-ALL-200993", "label": "PALM-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57379", "formula": "C37H62N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-201034", "reactome_id": "R-ALL-201034", "label": "L-PCAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17490", "formula": "C23H45NO4", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carnitine O-palmitoyltransferase activity of CPT1B [mitochondrial outer membrane]", "activity_id": "9911359", "ec_numbers": [ "2.3.1.21" ], "accessions": [ "Q92523" ] } ], "citations": [ { "id": "PMID:9344464", "title": "Functional studies of yeast-expressed human heart muscle carnitine palmitoyltransferase I", "journal": "Arch Biochem Biophys", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9344464/" } ], "x": 868.6666666666667, "y": 1594.859649122807, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9912889", "label": "SLC35D2 exchanges UDP-GlcA for UMP", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "Golgi membrane", "compartment_ids": [ "GO:0000139" ], "rhea_ids": [ "RHEA:72727" ], "source_url": "https://reactome.org/content/detail/R-HSA-9912889", "summary": "SLC35D2 exchanges UDP-GlcA for UMP is a Reactome release 97 curated reaction event. The source record connects UDP-GlcA, UMP to UDP-GlcA, UMP in Golgi membrane and cross-references RHEA:72727.", "equation": "UDP-GlcA + UMP → UDP-GlcA + UMP", "inputs": [ { "node_id": "R-ALL-158601", "reactome_id": "R-ALL-158601", "label": "UDP-GlcA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-735698", "reactome_id": "R-ALL-735698", "label": "UMP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57865", "formula": "C9H11N2O9P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-1889970", "reactome_id": "R-ALL-1889970", "label": "UDP-GlcA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58052", "formula": "C15H19N2O18P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-109402", "reactome_id": "R-ALL-109402", "label": "UMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57865", "formula": "C9H11N2O9P", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "UDP-glucuronate transmembrane transporter activity of SLC35D2 [Golgi membrane]", "activity_id": "9912903", "ec_numbers": null, "accessions": [ "Q76EJ3" ] } ], "citations": [ { "id": "PMID:15607426", "title": "Identification and characterization of human Golgi nucleotide sugar transporter SLC35D2, a novel member of the SLC35 nucleotide sugar transporter family", "journal": "Genomics", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15607426/" }, { "id": "PMID:17599910", "title": "Variety of nucleotide sugar transporters with respect to the interaction with nucleoside mono- and diphosphates", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17599910/" } ], "x": 7461.333333333333, "y": 2814.4411764705883, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9914143", "label": "SLC27A2-mediated ligation of peroxisomal fatty acid and CoASH", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "fatty-acid-metabolism", "peroxisomal-lipids" ], "compartment": "peroxisomal matrix", "compartment_ids": [ "GO:0005782" ], "rhea_ids": [ "RHEA:54537" ], "source_url": "https://reactome.org/content/detail/R-HSA-9914143", "summary": "SLC27A2-mediated ligation of peroxisomal fatty acid and CoASH is a Reactome release 97 curated reaction event. The source record connects ATP, CoA-SH, VLCFA to AMP, PPi, VLCFA-CoA in peroxisomal matrix and cross-references RHEA:54537.", "equation": "ATP + CoA-SH + VLCFA → AMP + PPi + VLCFA-CoA", "inputs": [ { "node_id": "R-ALL-389573", "reactome_id": "R-ALL-389573", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-193514", "reactome_id": "R-ALL-193514", "label": "CoA-SH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9914044", "reactome_id": "R-ALL-9914044", "label": "VLCFA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58950", "formula": "CO2R", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-389620", "reactome_id": "R-ALL-389620", "label": "AMP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456215", "formula": "C10H12N5O7P", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-389593", "reactome_id": "R-ALL-389593", "label": "PPi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:33019", "formula": "HO7P2", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9914131", "reactome_id": "R-ALL-9914131", "label": "VLCFA-CoA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:138261", "formula": "C22H31N7O17P3SR", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "very long-chain fatty acid-CoA ligase activity of SLC27A2 [peroxisomal membrane]", "activity_id": "9914116", "ec_numbers": null, "accessions": [ "O14975" ] } ], "citations": [ { "id": "PMID:10198260", "title": "Human very-long-chain acyl-CoA synthetase: cloning, topography, and relevance to branched-chain fatty acid metabolism", "journal": "Biochem Biophys Res Commun", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10198260/" } ], "x": 311.33333333333337, "y": 1543.561403508772, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9915968", "label": "8,9-EET is hydrolysed to 8.9-DHET by EPHX2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:44049" ], "source_url": "https://reactome.org/content/detail/R-HSA-9915968", "summary": "8,9-EET is hydrolysed to 8.9-DHET by EPHX2 is a Reactome release 97 curated reaction event. The source record connects H2O, 8,9-EET to 8,9-DHET in cytosol and cross-references RHEA:44049.", "equation": "H2O + 8,9-EET → 8,9-DHET", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142662", "reactome_id": "R-ALL-2142662", "label": "8,9-EET", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:84025", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142765", "reactome_id": "R-ALL-2142765", "label": "8,9-DHET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84032", "formula": "C20H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of EPHX2 dimer [cytosol]", "activity_id": "2161846", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:12468260", "title": "Characterization and identification of cytochrome P450 metabolites of arachidonic acid released by human peritoneal macrophages obtained from the pouch of Douglas", "journal": "Prostaglandins Leukot Essent Fatty Acids", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12468260/" }, { "id": "PMID:15096040", "title": "Structure of human epoxide hydrolase reveals mechanistic inferences on bifunctional catalysis in epoxide and phosphate ester hydrolysis", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15096040/" } ], "x": 311.33333333333337, "y": 1622.017543859649, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9915971", "label": "Arachidonate is epoxidated to 11,12-EET by CYP(5)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:51481" ], "source_url": "https://reactome.org/content/detail/R-HSA-9915971", "summary": "Arachidonate is epoxidated to 11,12-EET by CYP(5) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to NADP+, H2O, 11,12-EET in endoplasmic reticulum membrane · cytosol and cross-references RHEA:51481.", "equation": "AA + O2 + NADPH + H+ → NADP+ + H2O + 11,12-EET", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142835", "reactome_id": "R-ALL-2142835", "label": "11,12-EET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76625", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP(5) [endoplasmic reticulum membrane]", "activity_id": "2161882", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" }, { "id": "PMID:9866708", "title": "Analysis of cytochrome P450 metabolites of arachidonic and linoleic acids by liquid chromatography-mass spectrometry with ion trap MS", "journal": "Anal Biochem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9866708/" }, { "id": "PMID:7625847", "title": "Arachidonic acid metabolism by human cytochrome P450s 2C8, 2C9, 2E1, and 1A2: regioselective oxygenation and evidence for a role for CYP2C enzymes in arachidonic acid epoxygenation in human liver microsomes", "journal": "Arch Biochem Biophys", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7625847/" }, { "id": "PMID:8631948", "title": "Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631948/" } ], "x": 590, "y": 1658.2280701754385, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9915986", "label": "11,12-EET is hydrolysed to 11,12-DHET by EPHX2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:44045" ], "source_url": "https://reactome.org/content/detail/R-HSA-9915986", "summary": "11,12-EET is hydrolysed to 11,12-DHET by EPHX2 is a Reactome release 97 curated reaction event. The source record connects H2O, 11,12-EET to 11,12-DHET in cytosol and cross-references RHEA:44045.", "equation": "H2O + 11,12-EET → 11,12-DHET", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142835", "reactome_id": "R-ALL-2142835", "label": "11,12-EET", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:76625", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142779", "reactome_id": "R-ALL-2142779", "label": "11,12-DHET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84031", "formula": "C20H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of EPHX2 dimer [cytosol]", "activity_id": "2161846", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:12468260", "title": "Characterization and identification of cytochrome P450 metabolites of arachidonic acid released by human peritoneal macrophages obtained from the pouch of Douglas", "journal": "Prostaglandins Leukot Essent Fatty Acids", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12468260/" }, { "id": "PMID:15096040", "title": "Structure of human epoxide hydrolase reveals mechanistic inferences on bifunctional catalysis in epoxide and phosphate ester hydrolysis", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15096040/" } ], "x": 590, "y": 1622.017543859649, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9915992", "label": "Arachidonate is hydroxylated to 17-HETE by CYP(1)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:49969" ], "source_url": "https://reactome.org/content/detail/R-HSA-9915992", "summary": "Arachidonate is hydroxylated to 17-HETE by CYP(1) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to NADP+, H2O, 17-HETE in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:49969.", "equation": "AA + O2 + NADPH + H+ → NADP+ + H2O + 17-HETE", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142711", "reactome_id": "R-ALL-2142711", "label": "17-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132016", "formula": "C20H31O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP(1) [endoplasmic reticulum membrane]", "activity_id": "2161998", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" } ], "x": 868.6666666666667, "y": 1658.2280701754385, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9915993", "label": "Arachidonate is hydroxylated to 18-HETE by CYP(1)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · endoplasmic reticulum lumen", "compartment_ids": [ "GO:0005789", "GO:0005788" ], "rhea_ids": [ "RHEA:39812" ], "source_url": "https://reactome.org/content/detail/R-HSA-9915993", "summary": "Arachidonate is hydroxylated to 18-HETE by CYP(1) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to NADP+, H2O, 18-HETE in endoplasmic reticulum membrane · endoplasmic reticulum lumen and cross-references RHEA:39812.", "equation": "AA + O2 + NADPH + H+ → NADP+ + H2O + 18-HETE", "inputs": [ { "node_id": "R-ALL-140356", "reactome_id": "R-ALL-140356", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142830", "reactome_id": "R-ALL-2142830", "label": "18-HETE", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:63590", "formula": "C20H31O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP(1) [endoplasmic reticulum membrane]", "activity_id": "2161998", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" } ], "x": 311.33333333333337, "y": 1661.2456140350878, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9915994", "label": "14,15-EET is hydrolysed to 14,15-DHET by EPHX2", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:44041" ], "source_url": "https://reactome.org/content/detail/R-HSA-9915994", "summary": "14,15-EET is hydrolysed to 14,15-DHET by EPHX2 is a Reactome release 97 curated reaction event. The source record connects H2O, 14,15-EET to 14,15-DHET in cytosol and cross-references RHEA:44041.", "equation": "H2O + 14,15-EET → 14,15-DHET", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142792", "reactome_id": "R-ALL-2142792", "label": "14,15-EET", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:84024", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2142783", "reactome_id": "R-ALL-2142783", "label": "14,15-DHET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84029", "formula": "C20H33O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "epoxide hydrolase activity of EPHX2 dimer [cytosol]", "activity_id": "2161846", "ec_numbers": [ "3.3.2.10" ], "accessions": [] } ], "citations": [ { "id": "PMID:12468260", "title": "Characterization and identification of cytochrome P450 metabolites of arachidonic acid released by human peritoneal macrophages obtained from the pouch of Douglas", "journal": "Prostaglandins Leukot Essent Fatty Acids", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12468260/" }, { "id": "PMID:15096040", "title": "Structure of human epoxide hydrolase reveals mechanistic inferences on bifunctional catalysis in epoxide and phosphate ester hydrolysis", "journal": "Biochemistry", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15096040/" } ], "x": 868.6666666666667, "y": 1622.017543859649, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9915997", "label": "Arachidonate is epoxidated to 14,15-EET by CYP(5)", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "arachidonate", "fatty-acid-metabolism" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:51473" ], "source_url": "https://reactome.org/content/detail/R-HSA-9915997", "summary": "Arachidonate is epoxidated to 14,15-EET by CYP(5) is a Reactome release 97 curated reaction event. The source record connects AA, O2, NADPH to NADP+, H2O, 14,15-EET in endoplasmic reticulum membrane · cytosol and cross-references RHEA:51473.", "equation": "AA + O2 + NADPH + H+ → NADP+ + H2O + 14,15-EET", "inputs": [ { "node_id": "R-ALL-29768", "reactome_id": "R-ALL-29768", "label": "AA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:32395", "formula": "C20H31O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-2142792", "reactome_id": "R-ALL-2142792", "label": "14,15-EET", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84024", "formula": "C20H31O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "monooxygenase activity of CYP(5) [endoplasmic reticulum membrane]", "activity_id": "2161882", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:15258110", "title": "Metabolism of retinoids and arachidonic acid by human and mouse cytochrome P450 1b1", "journal": "Drug Metab Dispos", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15258110/" }, { "id": "PMID:9866708", "title": "Analysis of cytochrome P450 metabolites of arachidonic and linoleic acids by liquid chromatography-mass spectrometry with ion trap MS", "journal": "Anal Biochem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9866708/" }, { "id": "PMID:7625847", "title": "Arachidonic acid metabolism by human cytochrome P450s 2C8, 2C9, 2E1, and 1A2: regioselective oxygenation and evidence for a role for CYP2C enzymes in arachidonic acid epoxygenation in human liver microsomes", "journal": "Arch Biochem Biophys", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7625847/" }, { "id": "PMID:8631948", "title": "Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart", "journal": "J Biol Chem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8631948/" } ], "x": 590, "y": 1661.2456140350878, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9916401", "label": "ALOX12 oxidises LTA4 to LXB4", "schema_class": "Reaction", "domain_id": "lipid-mediators", "pathway_ids": [ "proresolving-mediators" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:48589" ], "source_url": "https://reactome.org/content/detail/R-HSA-9916401", "summary": "ALOX12 oxidises LTA4 to LXB4 is a Reactome release 97 curated reaction event. The source record connects LTA4, O2, H2O to LXB4 in cytosol and cross-references RHEA:48589.", "equation": "2 LTA4 + O2 + 2 H2O → 2 LXB4", "inputs": [ { "node_id": "R-ALL-9032038", "reactome_id": "R-ALL-9032038", "label": "LTA4", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:57463", "formula": "C20H29O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-2142776", "reactome_id": "R-ALL-2142776", "label": "LXB4", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:67031", "formula": "C20H31O5", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen of ALOX12:Fe2+ [cytosol]", "activity_id": "2161800", "ec_numbers": [ "1.13.11" ], "accessions": [] } ], "citations": [ { "id": "PMID:8250832", "title": "Lipoxin synthase activity of human platelet 12-lipoxygenase", "journal": "Biochem J", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8250832/" }, { "id": "PMID:2155925", "title": "Lipoxin formation during human neutrophil-platelet interactions. Evidence for the transformation of leukotriene A4 by platelet 12-lipoxygenase in vitro", "journal": "J Clin Invest", "year": 1990, "url": "https://pubmed.ncbi.nlm.nih.gov/2155925/" }, { "id": "PMID:3770188", "title": "Evidence for a 5(6)-epoxytetraene intermediate in the biosynthesis of lipoxins in human leukocytes. Conversion into lipoxin A by cytosolic epoxide hydrolase", "journal": "FEBS Lett", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3770188/" } ], "x": 5948.666666666667, "y": 2044.4166666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9929439", "label": "ALDH4A1 oxidizes 1PYR-3OH-5COOH", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "proline" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:31156" ], "source_url": "https://reactome.org/content/detail/R-HSA-9929439", "summary": "ALDH4A1 oxidizes 1PYR-3OH-5COOH is a Reactome release 97 curated reaction event. The source record connects 1PYR-3OH-5COOH, H2O, NAD+ to 4OH-L-Glu, H+, NADH in mitochondrial matrix and cross-references RHEA:31156.", "equation": "1PYR-3OH-5COOH + 2 H2O + NAD+ → 4OH-L-Glu + H+ + NADH", "inputs": [ { "node_id": "R-ALL-9929425", "reactome_id": "R-ALL-9929425", "label": "1PYR-3OH-5COOH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:62612", "formula": "C5H6NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 2, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9929459", "reactome_id": "R-ALL-9929459", "label": "4OH-L-Glu", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:6331", "formula": "C5H8NO5", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29362", "reactome_id": "R-ALL-29362", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "L-glutamate gamma-semialdehyde dehydrogenase activity of ALDH4A1 dimer [mitochondrial matrix]", "activity_id": "70678", "ec_numbers": [ "1.2.1.88" ], "accessions": [] } ], "citations": [ { "id": "PMID:13681370", "title": "Hydroxyproline metabolism. IV. Enzymatic synthesis of gamma-hydroxyglutamate from Delta 1-pyrroline-3-hydroxy-5-carboxylate", "journal": "J Biol Chem", "year": 1960, "url": "https://pubmed.ncbi.nlm.nih.gov/13681370/" }, { "id": "PMID:500817", "title": "Genetic evidence for a common enzyme catalyzing the second step in the degradation of proline and hydroxyproline", "journal": "J Clin Invest", "year": 1979, "url": "https://pubmed.ncbi.nlm.nih.gov/500817/" } ], "x": 6800, "y": 2288.875, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9929460", "label": "SDS dimers:PXLP convert L-Ser to PYR and NH4+", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "serine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:19170" ], "source_url": "https://reactome.org/content/detail/R-HSA-9929460", "summary": "SDS dimers:PXLP convert L-Ser to PYR and NH4+ is a Reactome release 97 curated reaction event. The source record connects L-Ser to NH4+, PYR in cytosol and cross-references RHEA:19170.", "equation": "L-Ser → NH4+ + PYR", "inputs": [ { "node_id": "R-ALL-200736", "reactome_id": "R-ALL-200736", "label": "L-Ser", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:33384", "formula": "C3H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29398", "reactome_id": "R-ALL-29398", "label": "PYR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15361", "formula": "C3H3O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-serine ammonia-lyase activity of PXLP-SDS dimers [cytosol]", "activity_id": "9929461", "ec_numbers": [ "4.3.1.17" ], "accessions": [] } ], "citations": [ { "id": "PMID:16580895", "title": "Enzymatic and biochemical properties of a novel human serine dehydratase isoform", "journal": "Biochim Biophys Acta", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16580895/" }, { "id": "PMID:18342636", "title": "A catalytic mechanism that explains a low catalytic activity of serine dehydratase like-1 from human cancer cells: crystal structure and site-directed mutagenesis studies", "journal": "Biochim. Biophys. Acta", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18342636/" } ], "x": 868.6666666666667, "y": 2917.6666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9931850", "label": "AKR1B1 reduces galactose to galactitol", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "galactose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:37969", "RHEA:12791" ], "source_url": "https://reactome.org/content/detail/R-HSA-9931850", "summary": "AKR1B1 reduces galactose to galactitol is a Reactome release 97 curated reaction event. The source record connects alpha-D-Gal, H+, NADPH to galactitol, NADP+ in cytosol and cross-references RHEA:37969, RHEA:12791.", "equation": "alpha-D-Gal + H+ + NADPH → galactitol + NADP+", "inputs": [ { "node_id": "R-ALL-9931851", "reactome_id": "R-ALL-9931851", "label": "alpha-D-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9931848", "reactome_id": "R-ALL-9931848", "label": "galactitol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16813", "formula": "C6H14O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldose reductase (NADPH) activity of AKR1B1 [cytosol]", "activity_id": "196106", "ec_numbers": [ "1.1.1.21" ], "accessions": [ "P15121" ] } ], "citations": [ { "id": "PMID:7851421", "title": "Presence of a closely related subgroup in the aldo-ketoreductase family of the mouse", "journal": "Eur J Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7851421/" }, { "id": "PMID:30362099", "title": "Aldo-Keto Reductases: Multifunctional Proteins as Therapeutic Targets in Diabetes and Inflammatory Disease", "journal": "Adv Exp Med Biol", "year": 2018, "url": "https://pubmed.ncbi.nlm.nih.gov/30362099/" }, { "id": "PMID:14456181", "title": "The accumulation of dulcitol and water in rabbit lens incubated with galactose", "journal": "Biochim Biophys Acta", "year": 1962, "url": "https://pubmed.ncbi.nlm.nih.gov/14456181/" }, { "id": "PMID:4270794", "title": "Polyol accumulation in galactosemic and diabetic rats: control by an aldose reductase inhibitor", "journal": "Science", "year": 1973, "url": "https://pubmed.ncbi.nlm.nih.gov/4270794/" }, { "id": "PMID:1907924", "title": "Polyol accumulation in cultured human lens epithelial cells", "journal": "Exp Eye Res", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1907924/" }, { "id": "PMID:12570032", "title": "The in vitro effects of galactose and its derivatives on rat brain Mg2+-ATPase activity", "journal": "Pharmacol Toxicol", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12570032/" } ], "x": 3600, "y": 360.25, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9931853", "label": "GALM converts beta-D-GAL to alpha-D-GAL", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "galactose" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:28677" ], "source_url": "https://reactome.org/content/detail/R-HSA-9931853", "summary": "GALM converts beta-D-GAL to alpha-D-GAL is a Reactome release 97 curated reaction event. The source record connects beta-D-Gal to alpha-D-Gal in cytosol and cross-references RHEA:28677.", "equation": "beta-D-Gal → alpha-D-Gal", "inputs": [ { "node_id": "R-ALL-9931855", "reactome_id": "R-ALL-9931855", "label": "beta-D-Gal", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27667", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9931851", "reactome_id": "R-ALL-9931851", "label": "alpha-D-Gal", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28061", "formula": "C6H12O6", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aldose 1-epimerase activity of GALM [cytosol]", "activity_id": "9931854", "ec_numbers": [ "5.1.3.3" ], "accessions": [ "Q96C23" ] } ], "citations": [ { "id": "PMID:12753898", "title": "Identification and characterisation of human aldose 1-epimerase", "journal": "FEBS Lett.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12753898/" }, { "id": "PMID:15026423", "title": "Molecular structure of human galactose mutarotase", "journal": "J Biol Chem", "year": 2004, "url": "https://pubmed.ncbi.nlm.nih.gov/15026423/" }, { "id": "PMID:7542884", "title": "Comparison of the enzymatic activities of human galactokinase GALK1 and a related human galactokinase protein GK2", "journal": "Biochem Biophys Res Commun", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/7542884/" } ], "x": 3600, "y": 554.75, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9938195", "label": "SLC25A51 mediates mitochondrial uptake of NAD+", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [ "RHEA:65408" ], "source_url": "https://reactome.org/content/detail/R-HSA-9938195", "summary": "SLC25A51 mediates mitochondrial uptake of NAD+ is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects NAD+ to NAD+ in cytosol · mitochondrial matrix and cross-references RHEA:65408.", "equation": "NAD+ → NAD+", "inputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "NAD transmembrane transporter activity of SLC25A51 [mitochondrial inner membrane]", "activity_id": "9938167", "ec_numbers": null, "accessions": [ "Q9H1U9" ] } ], "citations": [ { "id": "PMID:33087354", "title": "MCART1/SLC25A51 is required for mitochondrial NAD transport", "journal": "Sci Adv", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/33087354/" }, { "id": "PMID:32906142", "title": "SLC25A51 is a mammalian mitochondrial NAD + transporter", "journal": "Nature", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32906142/" } ], "x": 5670, "y": 2879.7763157894738, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9940993", "label": "PXYLP1 dephosphorylates Xyl moiety", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi lumen", "compartment_ids": [ "GO:0005796" ], "rhea_ids": [ "RHEA:56513" ], "source_url": "https://reactome.org/content/detail/R-HSA-9940993", "summary": "PXYLP1 dephosphorylates Xyl moiety is a Reactome release 97 curated reaction event. The source record connects GlcA-Gal-Gal-XylP-proteins, H2O to GlcA-Gal-Gal-Xyl-proteins, Pi in Golgi lumen and cross-references RHEA:56513.", "equation": "GlcA-Gal-Gal-XylP-proteins + H2O → GlcA-Gal-Gal-Xyl-proteins + Pi", "inputs": [ { "node_id": "R-HSA-9941158", "reactome_id": "R-HSA-9941158", "label": "GlcA-Gal-Gal-XylP-proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-2022884", "reactome_id": "R-ALL-2022884", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-2064225", "reactome_id": "R-HSA-2064225", "label": "GlcA-Gal-Gal-Xyl-proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-8851226", "reactome_id": "R-ALL-8851226", "label": "Pi", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:43474", "formula": "HO4P", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatase activity of PXYLP1 [Golgi membrane]", "activity_id": "9941202", "ec_numbers": [ "3.1.3" ], "accessions": [ "Q8TE99" ] } ], "citations": [ { "id": "PMID:31885391", "title": "Chondroitin sulfate metabolism in the brain", "journal": "Acta Neurobiol Exp (Wars)", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31885391/" }, { "id": "PMID:24425863", "title": "Identification of phosphatase that dephosphorylates xylose in the glycosaminoglycan-protein linkage region of proteoglycans", "journal": "J Biol Chem", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24425863/" } ], "x": 2660, "y": 901.1363636363636, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9941039", "label": "FAM20B phosphorylates Xyl moiety", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi lumen", "compartment_ids": [ "GO:0005796" ], "rhea_ids": [ "RHEA:19462" ], "source_url": "https://reactome.org/content/detail/R-HSA-9941039", "summary": "FAM20B phosphorylates Xyl moiety is a Reactome release 97 curated reaction event. The source record connects GlcA-Gal-Gal-Xyl-proteins, ATP to GlcA-Gal-Gal-XylP-proteins, ADP, H+ in Golgi lumen and cross-references RHEA:19462.", "equation": "GlcA-Gal-Gal-Xyl-proteins + ATP → GlcA-Gal-Gal-XylP-proteins + ADP + H+", "inputs": [ { "node_id": "R-HSA-2064225", "reactome_id": "R-HSA-2064225", "label": "GlcA-Gal-Gal-Xyl-proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-9941158", "reactome_id": "R-HSA-9941158", "label": "GlcA-Gal-Gal-XylP-proteins", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "phosphotransferase activity, alcohol group as acceptor of FAM20B [Golgi membrane]", "activity_id": "9941295", "ec_numbers": [ "2.7.1" ], "accessions": [ "O75063" ] } ], "citations": [ { "id": "PMID:19473117", "title": "FAM20B is a kinase that phosphorylates xylose in the glycosaminoglycan-protein linkage region", "journal": "Biochem J", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/19473117/" }, { "id": "PMID:31885391", "title": "Chondroitin sulfate metabolism in the brain", "journal": "Acta Neurobiol Exp (Wars)", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31885391/" } ], "x": 2938.666666666667, "y": 877.560606060606, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9941055", "label": "SPAM1 randomly cleaves HA", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9941055", "summary": "SPAM1 randomly cleaves HA is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects HA to HA, (HA)50 in extracellular region.", "equation": "HA → HA + (HA)50", "inputs": [ { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false }, { "node_id": "R-ALL-2160894", "reactome_id": "R-ALL-2160894", "label": "(HA)50", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Polymer", "currency": false } ], "catalysts": [ { "label": "hyalurononglucosaminidase activity of SPAM1 [plasma membrane]", "activity_id": "9941257", "ec_numbers": [ "3.2.1.35" ], "accessions": [ "P38567" ] } ], "citations": [ { "id": "PMID:11731269", "title": "The dual functions of GPI-anchored PH-20: hyaluronidase and intracellular signaling", "journal": "Matrix Biol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11731269/" }, { "id": "PMID:8282124", "title": "The human sperm protein PH-20 has hyaluronidase activity", "journal": "FEBS Lett", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8282124/" } ], "x": 2381.3333333333335, "y": 1089.7424242424242, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9941078", "label": "proHPSE dissociates from HS(6)-SDC1", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "lysosomal lumen", "compartment_ids": [ "GO:0043202" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9941078", "summary": "proHPSE dissociates from HS(6)-SDC1 is a Reactome release 97 curated reaction event. The source record connects proHPSE:SDC1 to proHPSE, HS(6)-SDC1 in lysosomal lumen.", "equation": "proHPSE:SDC1 → proHPSE + HS(6)-SDC1", "inputs": [ { "node_id": "R-HSA-9792577", "reactome_id": "R-HSA-9792577", "label": "proHPSE:SDC1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "Complex", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9792563", "reactome_id": "R-HSA-9792563", "label": "proHPSE", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-HSA-2090082", "reactome_id": "R-HSA-2090082", "label": "HS(6)-SDC1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "lysosomal lumen", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24788042", "title": "Processing of heparanase is mediated by syndecan-1 cytoplasmic domain and involves syntenin and α-actinin", "journal": "Cell Mol Life Sci", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24788042/" } ], "x": 2938.666666666667, "y": 1054.378787878788, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9941244", "label": "ROS/RNS degrade HA", "schema_class": "BlackBoxEvent", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "extracellular region", "compartment_ids": [ "GO:0005576" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9941244", "summary": "ROS/RNS degrade HA is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects ROS,RNS, HA to HA, (HA)50 in extracellular region.", "equation": "ROS,RNS + HA → HA + (HA)50", "inputs": [ { "node_id": "R-ALL-9941221", "reactome_id": "R-ALL-9941221", "label": "ROS,RNS", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false } ], "outputs": [ { "node_id": "R-ALL-2160848", "reactome_id": "R-ALL-2160848", "label": "HA", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "extracellular region", "entity_kind": "Polymer", "currency": false }, { "node_id": "R-ALL-2160894", "reactome_id": "R-ALL-2160894", "label": "(HA)50", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "plasma membrane", "entity_kind": "Polymer", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:16529395", "title": "Degradative action of reactive oxygen species on hyaluronan", "journal": "Biomacromolecules", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16529395/" }, { "id": "PMID:26448760", "title": "Roles of Proteoglycans and Glycosaminoglycans in Wound Healing and Fibrosis", "journal": "Int J Cell Biol", "year": 2015, "url": "https://pubmed.ncbi.nlm.nih.gov/26448760/" } ], "x": 2660, "y": 1125.1060606060605, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9941305", "label": "UST transfers sulfate to (2-)GlcA-GalNAc-6-sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi lumen", "compartment_ids": [ "GO:0005796" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9941305", "summary": "UST transfers sulfate to (2-)GlcA-GalNAc-6-sulfate is a Reactome release 97 curated reaction event. The source record connects C6S-PG, PAPS to C2S6S-PG, PAP, H+ in Golgi lumen.", "equation": "C6S-PG + PAPS → C2S6S-PG + PAP + H+", "inputs": [ { "node_id": "R-HSA-2064219", "reactome_id": "R-HSA-2064219", "label": "C6S-PG", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9941268", "reactome_id": "R-HSA-9941268", "label": "C2S6S-PG", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "chondroitin 2-O-sulfotransferase activity of UST [Golgi membrane]", "activity_id": "9941333", "ec_numbers": null, "accessions": [ "Q9Y2C2" ] } ], "citations": [ { "id": "PMID:10187838", "title": "Molecular cloning and characterization of a human uronyl 2-sulfotransferase that sulfates iduronyl and glucuronyl residues in dermatan/chondroitin sulfate", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10187838/" }, { "id": "PMID:17227754", "title": "Mutational study of heparan sulfate 2-O-sulfotransferase and chondroitin sulfate 2-O-sulfotransferase", "journal": "J Biol Chem", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17227754/" } ], "x": 2660, "y": 1019.0151515151515, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9941312", "label": "CHST15 transfers sulfate to (6-)GalNAc-4-sulfate", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:54301" ], "source_url": "https://reactome.org/content/detail/R-HSA-9941312", "summary": "CHST15 transfers sulfate to (6-)GalNAc-4-sulfate is a Reactome release 97 curated reaction event. The source record connects PAPS, D4S-PGs to PAP, H+, D4,6S-PGs in Golgi membrane · Golgi lumen and cross-references RHEA:54301.", "equation": "PAPS + D4S-PGs → PAP + H+ + D4,6S-PGs", "inputs": [ { "node_id": "R-ALL-741440", "reactome_id": "R-ALL-741440", "label": "PAPS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58339", "formula": "C10H11N5O13P2S", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2065135", "reactome_id": "R-HSA-2065135", "label": "D4S-PGs", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-742343", "reactome_id": "R-ALL-742343", "label": "PAP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58343", "formula": "C10H11N5O10P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9941241", "reactome_id": "R-HSA-9941241", "label": "D4,6S-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "catalysts": [ { "label": "N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase activity of CHST15 [Golgi membrane]", "activity_id": "2018675", "ec_numbers": [ "2.8.2.33" ], "accessions": [ "Q7LFX5" ] } ], "citations": [ { "id": "PMID:12874280", "title": "A unique nonreducing terminal modification of chondroitin sulfate by N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase", "journal": "J Biol Chem", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12874280/" } ], "x": 2381.3333333333335, "y": 889.3484848484849, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9941470", "label": "SLC26A11 imports SO4(2-) and H+ into cytosol", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "plasma membrane", "compartment_ids": [ "GO:0005886" ], "rhea_ids": [ "RHEA:28574" ], "source_url": "https://reactome.org/content/detail/R-HSA-9941470", "summary": "SLC26A11 imports SO4(2-) and H+ into cytosol is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects H+, SO4(2-) to H+, SO4(2-) in plasma membrane and cross-references RHEA:28574.", "equation": "H+ + SO4(2-) → H+ + SO4(2-)", "inputs": [ { "node_id": "R-ALL-351626", "reactome_id": "R-ALL-351626", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-427648", "reactome_id": "R-ALL-427648", "label": "SO4(2-)", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "extracellular region", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-174375", "reactome_id": "R-ALL-174375", "label": "SO4(2-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16189", "formula": "O4S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "sulfate transmembrane transporter activity of SLC26A11 [plasma membrane]", "activity_id": "8875870", "ec_numbers": null, "accessions": [ "Q86WA9" ] } ], "citations": [ { "id": "PMID:12626430", "title": "Molecular and functional characterization of SLC26A11, a sodium-independent sulfate transporter from high endothelial venules", "journal": "FASEB J.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12626430/" } ], "x": 2381.3333333333335, "y": 771.469696969697, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9945784", "label": "HSD17B7 reduces 4a-methyl-5a-cholest-8-en-3-one to 4a-methyl-5a-cholest-8-en-3b-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:46832", "RHEA:46833" ], "source_url": "https://reactome.org/content/detail/R-HSA-9945784", "summary": "HSD17B7 reduces 4a-methyl-5a-cholest-8-en-3-one to 4a-methyl-5a-cholest-8-en-3b-ol is a Reactome release 97 curated reaction event. The source record connects 4a-me-5a-cholest-8-en-3-one, H+, NADPH to 4a-me-5a-cholest-8-en-3b-ol, NADP+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:46832, RHEA:46833.", "equation": "4a-me-5a-cholest-8-en-3-one + H+ + NADPH → 4a-me-5a-cholest-8-en-3b-ol + NADP+", "inputs": [ { "node_id": "R-ALL-9945802", "reactome_id": "R-ALL-9945802", "label": "4a-me-5a-cholest-8-en-3-one", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:87050", "formula": "C28H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9945808", "reactome_id": "R-ALL-9945808", "label": "4a-me-5a-cholest-8-en-3b-ol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87051", "formula": "C28H48O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity of HSD17B7 [endoplasmic reticulum membrane]", "activity_id": "194731", "ec_numbers": [ "1.1.1.270" ], "accessions": [ "P56937" ] } ], "citations": [ { "id": "PMID:12829805", "title": "Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis", "journal": "Mol. Endocrinol.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12829805/" } ], "x": 2381.3333333333335, "y": 2154.633333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9945787", "label": "NSDHL decarboxylates 4a-carboxy-5a-cholest-8-ene-3b-ol to 5a-cholest-8-en-3-one", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:46848", "RHEA:46849" ], "source_url": "https://reactome.org/content/detail/R-HSA-9945787", "summary": "NSDHL decarboxylates 4a-carboxy-5a-cholest-8-ene-3b-ol to 5a-cholest-8-en-3-one is a Reactome release 97 curated reaction event. The source record connects 4a-carboxy-5a-cholest-8-en-3b-ol, NADP+ to 5a-cholest-8-en-3-one, CO2, NADPH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:46848, RHEA:46849.", "equation": "4a-carboxy-5a-cholest-8-en-3b-ol + NADP+ → 5a-cholest-8-en-3-one + CO2 + NADPH", "inputs": [ { "node_id": "R-ALL-9945792", "reactome_id": "R-ALL-9945792", "label": "4a-carboxy-5a-cholest-8-en-3b-ol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:87055", "formula": "C28H45O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9945794", "reactome_id": "R-ALL-9945794", "label": "5a-cholest-8-en-3-one", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87056", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity of NSDHL [endoplasmic reticulum membrane]", "activity_id": "194724", "ec_numbers": [ "1.1.1.170" ], "accessions": [ "Q15738" ] } ], "citations": [ { "id": "PMID:10369263", "title": "The gene mutated in bare patches and striated mice encodes a novel 3beta-hydroxysteroid dehydrogenase", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10369263/" } ], "x": 2660, "y": 2154.633333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9945804", "label": "HSD17B7 reduces 5a-cholest-8-en-3-one to ZYMSTNL", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:46852", "RHEA:46853" ], "source_url": "https://reactome.org/content/detail/R-HSA-9945804", "summary": "HSD17B7 reduces 5a-cholest-8-en-3-one to ZYMSTNL is a Reactome release 97 curated reaction event. The source record connects 5a-cholest-8-en-3-one, H+, NADPH to NADP+, ZYMSTNL in endoplasmic reticulum membrane · cytosol and cross-references RHEA:46852, RHEA:46853.", "equation": "5a-cholest-8-en-3-one + H+ + NADPH → NADP+ + ZYMSTNL", "inputs": [ { "node_id": "R-ALL-9945794", "reactome_id": "R-ALL-9945794", "label": "5a-cholest-8-en-3-one", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:87056", "formula": "C27H44O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-6807048", "reactome_id": "R-ALL-6807048", "label": "ZYMSTNL", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16608", "formula": "C27H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "3-beta-hydroxysteroid 3-dehydrogenase (NADP+) activity of HSD17B7 [endoplasmic reticulum membrane]", "activity_id": "194731", "ec_numbers": [ "1.1.1.270" ], "accessions": [ "P56937" ] } ], "citations": [ { "id": "PMID:12829805", "title": "Closing the gap: identification of human 3-ketosteroid reductase, the last unknown enzyme of mammalian cholesterol biosynthesis", "journal": "Mol. Endocrinol.", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/12829805/" } ], "x": 2938.666666666667, "y": 2154.633333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9945822", "label": "NSDHL decarboxylates 4a-carboxy-4b-methyl-5a-cholest-8-en-3b-ol to 4a-methyl-5a-cholest-8-en-3b-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:46828", "RHEA:46829" ], "source_url": "https://reactome.org/content/detail/R-HSA-9945822", "summary": "NSDHL decarboxylates 4a-carboxy-4b-methyl-5a-cholest-8-en-3b-ol to 4a-methyl-5a-cholest-8-en-3b-ol is a Reactome release 97 curated reaction event. The source record connects 4a-carboxy-4b-me-5a-cholest-8-en-3b-ol, NADP+ to 4a-me-5a-cholest-8-en-3-one, CO2, NADPH in endoplasmic reticulum membrane · cytosol and cross-references RHEA:46828, RHEA:46829.", "equation": "4a-carboxy-4b-me-5a-cholest-8-en-3b-ol + NADP+ → 4a-me-5a-cholest-8-en-3-one + CO2 + NADPH", "inputs": [ { "node_id": "R-ALL-9945820", "reactome_id": "R-ALL-9945820", "label": "4a-carboxy-4b-me-5a-cholest-8-en-3b-ol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:87047", "formula": "C29H47O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9945802", "reactome_id": "R-ALL-9945802", "label": "4a-me-5a-cholest-8-en-3-one", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87050", "formula": "C28H46O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113528", "reactome_id": "R-ALL-113528", "label": "CO2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16526", "formula": "CO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "3-beta-hydroxysteroid dehydrogenase [NAD(P)+]/C4-decarboxylase activity of NSDHL [endoplasmic reticulum membrane]", "activity_id": "194724", "ec_numbers": [ "1.1.1.170" ], "accessions": [ "Q15738" ] } ], "citations": [ { "id": "PMID:10369263", "title": "The gene mutated in bare patches and striated mice encodes a novel 3beta-hydroxysteroid dehydrogenase", "journal": "Nat Genet", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10369263/" } ], "x": 2381.3333333333335, "y": 2180.5666666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9946140", "label": "TM7SF2 reduces 4,4-diMeCholesta-8,14-dien-3-ol to 4,4-diMe5-cholest-8-en-3-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:46812", "RHEA:46813" ], "source_url": "https://reactome.org/content/detail/R-HSA-9946140", "summary": "TM7SF2 reduces 4,4-diMeCholesta-8,14-dien-3-ol to 4,4-diMe5-cholest-8-en-3-ol is a Reactome release 97 curated reaction event. The source record connects 4,4-diMe-8,14-cholestadien-3b-ol, H+, NADPH to 4,4-diMe-5-cholest-8-en-3-ol, NADP+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:46812, RHEA:46813.", "equation": "4,4-diMe-8,14-cholestadien-3b-ol + H+ + NADPH → 4,4-diMe-5-cholest-8-en-3-ol + NADP+", "inputs": [ { "node_id": "R-ALL-9946142", "reactome_id": "R-ALL-9946142", "label": "4,4-diMe-8,14-cholestadien-3b-ol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:78904", "formula": "C29H48O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29364", "reactome_id": "R-ALL-29364", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57783", "formula": "C21H26N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-9946141", "reactome_id": "R-ALL-9946141", "label": "4,4-diMe-5-cholest-8-en-3-ol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87044", "formula": "C29H50O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29366", "reactome_id": "R-ALL-29366", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58349", "formula": "C21H25N7O17P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "Delta14-sterol reductase activity of TM7SF2 [endoplasmic reticulum membrane]", "activity_id": "194714", "ec_numbers": [ "1.3.1.70" ], "accessions": [ "O76062" ] } ], "citations": [ { "id": "PMID:16784888", "title": "Sterol dependent regulation of human TM7SF2 gene expression: role of the encoded 3beta-hydroxysterol Delta14-reductase in human cholesterol biosynthesis", "journal": "Biochim Biophys Acta", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16784888/" } ], "x": 2660, "y": 2180.5666666666666, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9947148", "label": "CYP51A1 14-demethylates 24,25-dhLAN to 4,4-diMeCholesta-8,14-dien-3-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:45961" ], "source_url": "https://reactome.org/content/detail/R-HSA-9947148", "summary": "CYP51A1 14-demethylates 24,25-dhLAN to 4,4-diMeCholesta-8,14-dien-3-ol is a Reactome release 97 curated reaction event. The source record connects 24,25-dhLAN, CYP450:Fe2+, O2 to 4,4-diMe-8,14-cholestadien-3b-ol, HCOOH, CYP450:Fe3+ in endoplasmic reticulum membrane · cytosol and cross-references RHEA:45961.", "equation": "24,25-dhLAN + 3 CYP450:Fe2+ + 3 O2 → 4,4-diMe-8,14-cholestadien-3b-ol + HCOOH + 3 CYP450:Fe3+ + 4 H+ + 4 H2O", "inputs": [ { "node_id": "R-ALL-1130708", "reactome_id": "R-ALL-1130708", "label": "24,25-dhLAN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28113", "formula": "C30H52O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-8957354", "reactome_id": "R-HSA-8957354", "label": "CYP450:Fe2+", "role": "input", "stoichiometry": 3, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9946142", "reactome_id": "R-ALL-9946142", "label": "4,4-diMe-8,14-cholestadien-3b-ol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:78904", "formula": "C29H48O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29460", "reactome_id": "R-ALL-29460", "label": "HCOOH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15740", "formula": "CHO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-8957351", "reactome_id": "R-HSA-8957351", "label": "CYP450:Fe3+", "role": "output", "stoichiometry": 3, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "sterol 14-demethylase activity of CYP51A1 [endoplasmic reticulum membrane]", "activity_id": "9947144", "ec_numbers": [ "1.14.14.154" ], "accessions": [ "Q16850" ] } ], "citations": [ { "id": "PMID:8797093", "title": "Sterol 14-demethylase P450 (P45014DM*) is one of the most ancient and conserved P450 species", "journal": "J Biochem", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8797093/" }, { "id": "PMID:8399332", "title": "Purification of a human cytochrome P-450 isozyme catalyzing lanosterol 14 alpha-demethylation", "journal": "Biochim Biophys Acta", "year": 1993, "url": "https://pubmed.ncbi.nlm.nih.gov/8399332/" }, { "id": "PMID:8619637", "title": "The ubiquitously expressed human CYP51 encodes lanosterol 14 alpha-demethylase, a cytochrome P450 whose expression is regulated by oxysterols", "journal": "Arch Biochem Biophys", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8619637/" } ], "x": 2938.666666666667, "y": 2336.1666666666665, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9947203", "label": "MSMO1 oxidizes 4,4-diMe-5-cholest-8-en-3-ol to 4a-carboxy-4b-me-5a-cholest-8-en-3b-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:62777" ], "source_url": "https://reactome.org/content/detail/R-HSA-9947203", "summary": "MSMO1 oxidizes 4,4-diMe-5-cholest-8-en-3-ol to 4a-carboxy-4b-me-5a-cholest-8-en-3b-ol is a Reactome release 97 curated reaction event. The source record connects 4,4-diMe-5-cholest-8-en-3-ol, CYP450:Fe2+, H+ to 4a-carboxy-4b-me-5a-cholest-8-en-3b-ol, CYP450:Fe3+, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:62777.", "equation": "4,4-diMe-5-cholest-8-en-3-ol + 6 CYP450:Fe2+ + 5 H+ + 3 O2 → 4a-carboxy-4b-me-5a-cholest-8-en-3b-ol + 6 CYP450:Fe3+ + 4 H2O", "inputs": [ { "node_id": "R-ALL-9946141", "reactome_id": "R-ALL-9946141", "label": "4,4-diMe-5-cholest-8-en-3-ol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:87044", "formula": "C29H50O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-8957354", "reactome_id": "R-HSA-8957354", "label": "CYP450:Fe2+", "role": "input", "stoichiometry": 6, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 5, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9945820", "reactome_id": "R-ALL-9945820", "label": "4a-carboxy-4b-me-5a-cholest-8-en-3b-ol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87047", "formula": "C29H47O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-8957351", "reactome_id": "R-HSA-8957351", "label": "CYP450:Fe3+", "role": "output", "stoichiometry": 6, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "C-4 methylsterol oxidase activity of MSMO1 [endoplasmic reticulum membrane]", "activity_id": "194699", "ec_numbers": [ "1.14.18.9" ], "accessions": [ "Q15800" ] } ], "citations": [ { "id": "PMID:21285510", "title": "Mutations in the human SC4MOL gene encoding a methyl sterol oxidase cause psoriasiform dermatitis, microcephaly, and developmental delay", "journal": "J Clin Invest", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21285510/" } ], "x": 2938.666666666667, "y": 2362.1, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9947207", "label": "MSMO1 oxidizes 4a-me-5a-cholest-8-en-3b-ol to 4a-carboxy-5a-cholest-8-ene-3b-ol", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "cholesterol", "steroids" ], "compartment": "endoplasmic reticulum membrane · cytosol", "compartment_ids": [ "GO:0005789", "GO:0005829" ], "rhea_ids": [ "RHEA:62781" ], "source_url": "https://reactome.org/content/detail/R-HSA-9947207", "summary": "MSMO1 oxidizes 4a-me-5a-cholest-8-en-3b-ol to 4a-carboxy-5a-cholest-8-ene-3b-ol is a Reactome release 97 curated reaction event. The source record connects 4a-me-5a-cholest-8-en-3b-ol, CYP450:Fe2+, H+ to 4a-carboxy-5a-cholest-8-en-3b-ol, CYP450:Fe3+, H2O in endoplasmic reticulum membrane · cytosol and cross-references RHEA:62781.", "equation": "4a-me-5a-cholest-8-en-3b-ol + 6 CYP450:Fe2+ + 5 H+ + 3 O2 → 4a-carboxy-5a-cholest-8-en-3b-ol + 6 CYP450:Fe3+ + 4 H2O", "inputs": [ { "node_id": "R-ALL-9945808", "reactome_id": "R-ALL-9945808", "label": "4a-me-5a-cholest-8-en-3b-ol", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:87051", "formula": "C28H48O", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-8957354", "reactome_id": "R-HSA-8957354", "label": "CYP450:Fe2+", "role": "input", "stoichiometry": 6, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 5, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 3, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9945792", "reactome_id": "R-ALL-9945792", "label": "4a-carboxy-5a-cholest-8-en-3b-ol", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87055", "formula": "C28H45O3", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-8957351", "reactome_id": "R-HSA-8957351", "label": "CYP450:Fe3+", "role": "output", "stoichiometry": 6, "chebi_id": "", "formula": "", "compartment": "endoplasmic reticulum membrane", "entity_kind": "Complex", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 4, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "C-4 methylsterol oxidase activity of MSMO1 [endoplasmic reticulum membrane]", "activity_id": "194699", "ec_numbers": [ "1.14.18.9" ], "accessions": [ "Q15800" ] } ], "citations": [ { "id": "PMID:21285510", "title": "Mutations in the human SC4MOL gene encoding a methyl sterol oxidase cause psoriasiform dermatitis, microcephaly, and developmental delay", "journal": "J Clin Invest", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/21285510/" } ], "x": 2381.3333333333335, "y": 2388.0333333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9953259", "label": "EXTL3 dimer transfers GlcNAc to the GAG linker", "schema_class": "Reaction", "domain_id": "sugar-branches-glycans", "pathway_ids": [ "glycosaminoglycans" ], "compartment": "Golgi membrane · Golgi lumen", "compartment_ids": [ "GO:0000139", "GO:0005796" ], "rhea_ids": [ "RHEA:16222" ], "source_url": "https://reactome.org/content/detail/R-HSA-9953259", "summary": "EXTL3 dimer transfers GlcNAc to the GAG linker is a Reactome release 97 curated reaction event. The source record connects UDP-GlcNAc, GlcA-Gal-Gal-Xyl-HS proteins to UDP, Heparan-PGs, H+ in Golgi membrane · Golgi lumen and cross-references RHEA:16222.", "equation": "UDP-GlcNAc + GlcA-Gal-Gal-Xyl-HS proteins → UDP + Heparan-PGs + H+", "inputs": [ { "node_id": "R-ALL-914003", "reactome_id": "R-ALL-914003", "label": "UDP-GlcNAc", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57705", "formula": "C17H25N3O17P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076551", "reactome_id": "R-HSA-2076551", "label": "GlcA-Gal-Gal-Xyl-HS proteins", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-205687", "reactome_id": "R-ALL-205687", "label": "UDP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58223", "formula": "C9H11N2O12P2", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-2076465", "reactome_id": "R-HSA-2076465", "label": "Heparan-PGs", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "Golgi lumen", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-425969", "reactome_id": "R-ALL-425969", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "Golgi lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase activity of EXTL2,3 [Golgi membrane]", "activity_id": "9941289", "ec_numbers": [ "2.4.1.223" ], "accessions": [] } ], "citations": [ { "id": "PMID:11390981", "title": "Human tumor suppressor EXT gene family members EXTL1 and EXTL3 encode alpha 1,4- N-acetylglucosaminyltransferases that likely are involved in heparan sulfate/ heparin biosynthesis", "journal": "Proc Natl Acad Sci U S A", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11390981/" }, { "id": "PMID:35676258", "title": "The structure of EXTL3 helps to explain the different roles of bi-domain exostosins in heparan sulfate synthesis", "journal": "Nat Commun", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35676258/" }, { "id": "PMID:28132690", "title": "Mutations in EXTL3 Cause Neuro-immuno-skeletal Dysplasia Syndrome", "journal": "Am J Hum Genet", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28132690/" }, { "id": "PMID:28148688", "title": "EXTL3 mutations cause skeletal dysplasia, immune deficiency, and developmental delay", "journal": "J Exp Med", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28148688/" }, { "id": "PMID:10318803", "title": "The tumor suppressor EXT-like gene EXTL2 encodes an alpha1, 4-N-acetylhexosaminyltransferase that transfers N-acetylgalactosamine and N-acetylglucosamine to the common glycosaminoglycan-protein linkage region. The key enzyme for the chain initiation of heparan sulfate", "journal": "J Biol Chem", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10318803/" } ], "x": 2660, "y": 889.3484848484849, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9955504", "label": "SIRT5 deglutarylates CPS1", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:47665" ], "source_url": "https://reactome.org/content/detail/R-HSA-9955504", "summary": "SIRT5 deglutarylates CPS1 is a Reactome release 97 curated reaction event. The source record connects N6-glutaryl lysine CPS1, NAD+, H2O to CPS1, 2''-O-glutaryl-ADP-D-ribose(3-), NAM in mitochondrial matrix and cross-references RHEA:47665.", "equation": "N6-glutaryl lysine CPS1 + 8 NAD+ + 8 H2O → CPS1 + 8 2''-O-glutaryl-ADP-D-ribose(3-) + 8 NAM", "inputs": [ { "node_id": "R-HSA-9955510", "reactome_id": "R-HSA-9955510", "label": "N6-glutaryl lysine CPS1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 8, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-HSA-70544", "reactome_id": "R-HSA-70544", "label": "CPS1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false }, { "node_id": "R-ALL-9955515", "reactome_id": "R-ALL-9955515", "label": "2''-O-glutaryl-ADP-D-ribose(3-)", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:87829", "formula": "C20H26N5O17P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5688274", "reactome_id": "R-ALL-5688274", "label": "NAM", "role": "output", "stoichiometry": 8, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "protein-glutaryllysine deglutarylase activity of SIRT5 [mitochondrial matrix]", "activity_id": "9955442", "ec_numbers": null, "accessions": [ "Q9NXA8" ] } ], "citations": [ { "id": "PMID:24703693", "title": "Lysine glutarylation is a protein posttranslational modification regulated by SIRT5", "journal": "Cell Metab", "year": 2014, "url": "https://pubmed.ncbi.nlm.nih.gov/24703693/" } ], "x": 1808.6666666666667, "y": 3217.888888888889, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9955518", "label": "SIRT5 desuccinylates CPS1", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:47669" ], "source_url": "https://reactome.org/content/detail/R-HSA-9955518", "summary": "SIRT5 desuccinylates CPS1 is a Reactome release 97 curated reaction event. The source record connects NAD+, H2O, N6-succinyl K1291 CPS1 to NAM, 2''-O-succinyl-ADP-D-ribose(3-), CPS1 in mitochondrial matrix and cross-references RHEA:47669.", "equation": "NAD+ + H2O + N6-succinyl K1291 CPS1 → NAM + 2''-O-succinyl-ADP-D-ribose(3-) + CPS1", "inputs": [ { "node_id": "R-ALL-113526", "reactome_id": "R-ALL-113526", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113521", "reactome_id": "R-ALL-113521", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-HSA-9955506", "reactome_id": "R-HSA-9955506", "label": "N6-succinyl K1291 CPS1", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-ALL-5688274", "reactome_id": "R-ALL-5688274", "label": "NAM", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:17154", "formula": "C6H6N2O", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9955509", "reactome_id": "R-ALL-9955509", "label": "2''-O-succinyl-ADP-D-ribose(3-)", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:87832", "formula": "C19H24N5O17P2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-HSA-70544", "reactome_id": "R-HSA-70544", "label": "CPS1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [ { "label": "protein-succinyllysine desuccinylase activity of SIRT5 [mitochondrial matrix]", "activity_id": "9955438", "ec_numbers": null, "accessions": [ "Q9NXA8" ] } ], "citations": [ { "id": "PMID:22076378", "title": "Sirt5 is a NAD-dependent protein lysine demalonylase and desuccinylase", "journal": "Science", "year": 2011, "url": "https://pubmed.ncbi.nlm.nih.gov/22076378/" }, { "id": "PMID:23954790", "title": "Lysine succinylation is a frequently occurring modification in prokaryotes and eukaryotes and extensively overlaps with acetylation", "journal": "Cell Rep", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23954790/" } ], "x": 1251.3333333333333, "y": 3256.1111111111113, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9956308", "label": "STARD7 transports PC from ER membrane to mitochondrial outer membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "phospholipids" ], "compartment": "endoplasmic reticulum membrane · mitochondrial outer membrane", "compartment_ids": [ "GO:0005789", "GO:0005741" ], "rhea_ids": [ "RHEA:38571" ], "source_url": "https://reactome.org/content/detail/R-HSA-9956308", "summary": "STARD7 transports PC from ER membrane to mitochondrial outer membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects PC to PC in endoplasmic reticulum membrane · mitochondrial outer membrane and cross-references RHEA:38571.", "equation": "PC → PC", "inputs": [ { "node_id": "R-ALL-426925", "reactome_id": "R-ALL-426925", "label": "PC", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-8873803", "reactome_id": "R-ALL-8873803", "label": "PC", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57643", "formula": "C10H18NO8PR2", "compartment": "mitochondrial outer membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "phosphatidylcholine transfer activity of STARD7 [mitochondrial outer membrane]", "activity_id": "9956301", "ec_numbers": null, "accessions": [ "Q9NQZ5" ] } ], "citations": [ { "id": "PMID:28821867", "title": "Identification of the N-terminal transmembrane domain of StarD7 and its importance for mitochondrial outer membrane localization and phosphatidylcholine transfer", "journal": "Sci Rep", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28821867/" } ], "x": 2938.666666666667, "y": 1535.664383561644, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9956502", "label": "OTC precursor is targeted to the mitochondrial matrix and processed", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "cytosol · mitochondrial matrix", "compartment_ids": [ "GO:0005829", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9956502", "summary": "OTC precursor is targeted to the mitochondrial matrix and processed is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects OTC precursor to OTC trimer in cytosol · mitochondrial matrix.", "equation": "OTC precursor → OTC trimer", "inputs": [ { "node_id": "R-HSA-9956408", "reactome_id": "R-HSA-9956408", "label": "OTC precursor", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-70558", "reactome_id": "R-HSA-70558", "label": "OTC trimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:6372096", "title": "Structure and expression of a complementary DNA for the nuclear coded precursor of human mitochondrial ornithine transcarbamylase.", "journal": "Science", "year": 1984, "url": "https://pubmed.ncbi.nlm.nih.gov/6372096/" }, { "id": "PMID:28301740", "title": "Mitochondrial Machineries for Protein Import and Assembly", "journal": "Annu. Rev. Biochem.", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28301740/" }, { "id": "PMID:9852088", "title": "1.85-A resolution crystal structure of human ornithine transcarbamoylase complexed with N-phosphonacetyl-L-ornithine. Catalytic mechanism and correlation with inherited deficiency", "journal": "J Biol Chem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9852088/" }, { "id": "PMID:10813810", "title": "Crystal structure of human ornithine transcarbamylase complexed with carbamoyl phosphate and L-norvaline at 1.9 A resolution", "journal": "Proteins", "year": 2000, "url": "https://pubmed.ncbi.nlm.nih.gov/10813810/" } ], "x": 1530, "y": 3294.3333333333335, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9956909", "label": "CERT1-2 transfers CERA from the ER membrane to the Golgi membrane", "schema_class": "Reaction", "domain_id": "complex-lipids", "pathway_ids": [ "sphingolipids" ], "compartment": "Golgi membrane · endoplasmic reticulum membrane", "compartment_ids": [ "GO:0000139", "GO:0005789" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9956909", "summary": "CERT1-2 transfers CERA from the ER membrane to the Golgi membrane is a Reactome release 97 curated event with participant identities in more than one compartment. The source record connects CERA to CERA in Golgi membrane · endoplasmic reticulum membrane.", "equation": "CERA → CERA", "inputs": [ { "node_id": "R-ALL-428256", "reactome_id": "R-ALL-428256", "label": "CERA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "endoplasmic reticulum membrane", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-428270", "reactome_id": "R-ALL-428270", "label": "CERA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:83273", "formula": "C4H7NO3R2", "compartment": "Golgi membrane", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "ceramide transfer activity of CERT1-2:PPM1L:VAPA/B dimer [endoplasmic reticulum membrane]", "activity_id": "9956911", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:26601944", "title": "Oxysterol-binding Protein Activation at Endoplasmic Reticulum-Golgi Contact Sites Reorganizes Phosphatidylinositol 4-Phosphate Pools", "journal": "J Biol Chem", "year": 2016, "url": "https://pubmed.ncbi.nlm.nih.gov/26601944/" }, { "id": "PMID:14685229", "title": "Molecular machinery for non-vesicular trafficking of ceramide", "journal": "Nature", "year": 2003, "url": "https://pubmed.ncbi.nlm.nih.gov/14685229/" }, { "id": "PMID:16895911", "title": "Efficient trafficking of ceramide from the endoplasmic reticulum to the Golgi apparatus requires a VAMP-associated protein-interacting FFAT motif of CERT", "journal": "J Biol Chem", "year": 2006, "url": "https://pubmed.ncbi.nlm.nih.gov/16895911/" }, { "id": "PMID:18184806", "title": "Structural basis for specific lipid recognition by CERT responsible for nonvesicular trafficking of ceramide", "journal": "Proc Natl Acad Sci U S A", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18184806/" }, { "id": "PMID:31254361", "title": "Structure, functions and regulation of CERT, a lipid-transfer protein for the delivery of ceramide at the ER-Golgi membrane contact sites", "journal": "FEBS Lett", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/31254361/" }, { "id": "PMID:22226883", "title": "Integration of non-vesicular and vesicular transport processes at the Golgi complex by the PKD-CERT network", "journal": "Biochim Biophys Acta", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22226883/" } ], "x": 3321.3333333333335, "y": 1869.6346153846155, "lod": "pathway", "collapsed": false, "transport": true }, { "id": "R-HSA-9959857", "label": "TP53 binds ARG1 gene", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9959857", "summary": "TP53 binds ARG1 gene is a Reactome release 97 curated reaction event. The source record connects TP53 Tetramer, ARG1 gene to TP53 tetramer:ARG1 gene in nucleoplasm.", "equation": "TP53 Tetramer + ARG1 gene → TP53 tetramer:ARG1 gene", "inputs": [ { "node_id": "R-HSA-3209194", "reactome_id": "R-HSA-3209194", "label": "TP53 Tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9959833", "reactome_id": "R-HSA-9959833", "label": "ARG1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9959839", "reactome_id": "R-HSA-9959839", "label": "TP53 tetramer:ARG1 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription repressor activity of TP53 Tetramer [nucleoplasm]", "activity_id": "9988056", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:30842655", "title": "p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis", "journal": "Nature", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30842655/" }, { "id": "PMID:35477776", "title": "Polyamines in cancer: integrating organismal metabolism and antitumour immunity", "journal": "Nat Rev Cancer", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35477776/" } ], "x": 1530, "y": 3256.1111111111113, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9959860", "label": "TP53 binds CPS1 gene", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9959860", "summary": "TP53 binds CPS1 gene is a Reactome release 97 curated reaction event. The source record connects TP53 Tetramer, CPS1 gene to TP53 tetramer:CPS1 gene in nucleoplasm.", "equation": "TP53 Tetramer + CPS1 gene → TP53 tetramer:CPS1 gene", "inputs": [ { "node_id": "R-HSA-3209194", "reactome_id": "R-HSA-3209194", "label": "TP53 Tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9959836", "reactome_id": "R-HSA-9959836", "label": "CPS1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9959843", "reactome_id": "R-HSA-9959843", "label": "TP53 tetramer:CPS1 gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription repressor activity of TP53 Tetramer [nucleoplasm]", "activity_id": "9988056", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:30842655", "title": "p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis", "journal": "Nature", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30842655/" }, { "id": "PMID:35477776", "title": "Polyamines in cancer: integrating organismal metabolism and antitumour immunity", "journal": "Nat Rev Cancer", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35477776/" } ], "x": 1808.6666666666667, "y": 3256.1111111111113, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9959863", "label": "TP53 binds OTC gene", "schema_class": "Reaction", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "nucleoplasm", "compartment_ids": [ "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9959863", "summary": "TP53 binds OTC gene is a Reactome release 97 curated reaction event. The source record connects TP53 Tetramer, OTC gene to TP53 tetramer:OTC gene in nucleoplasm.", "equation": "TP53 Tetramer + OTC gene → TP53 tetramer:OTC gene", "inputs": [ { "node_id": "R-HSA-3209194", "reactome_id": "R-HSA-3209194", "label": "TP53 Tetramer", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false }, { "node_id": "R-HSA-9959831", "reactome_id": "R-HSA-9959831", "label": "OTC gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9959846", "reactome_id": "R-HSA-9959846", "label": "TP53 tetramer:OTC gene", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "Complex", "currency": false } ], "catalysts": [ { "label": "DNA-binding transcription repressor activity of TP53 Tetramer [nucleoplasm]", "activity_id": "9988056", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:30842655", "title": "p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis", "journal": "Nature", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30842655/" }, { "id": "PMID:35477776", "title": "Polyamines in cancer: integrating organismal metabolism and antitumour immunity", "journal": "Nat Rev Cancer", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35477776/" } ], "x": 1251.3333333333333, "y": 3294.3333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9959871", "label": "ARG1 gene expression", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "cytoplasm · nucleoplasm", "compartment_ids": [ "GO:0005737", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9959871", "summary": "ARG1 gene expression is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects ARG1 gene to ARG1 trimer in cytoplasm · nucleoplasm.", "equation": "ARG1 gene → ARG1 trimer", "inputs": [ { "node_id": "R-HSA-9959833", "reactome_id": "R-HSA-9959833", "label": "ARG1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-70567", "reactome_id": "R-HSA-70567", "label": "ARG1 trimer", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "Complex", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:30842655", "title": "p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis", "journal": "Nature", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30842655/" }, { "id": "PMID:17900240", "title": "Human ornithine decarboxylase paralogue (ODCp) is an antizyme inhibitor but not an arginine decarboxylase", "journal": "Biochem J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17900240/" }, { "id": "PMID:17407445", "title": "A structural insight into the inhibition of human and Leishmania donovani ornithine decarboxylases by 1-amino-oxy-3-aminopropane", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17407445/" }, { "id": "PMID:35477776", "title": "Polyamines in cancer: integrating organismal metabolism and antitumour immunity", "journal": "Nat Rev Cancer", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35477776/" } ], "x": 1808.6666666666667, "y": 3294.3333333333335, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9959874", "label": "CPS1 gene expression", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "cytoplasm · nucleoplasm", "compartment_ids": [ "GO:0005737", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9959874", "summary": "CPS1 gene expression is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects CPS1 gene to CPS1 in cytoplasm · nucleoplasm.", "equation": "CPS1 gene → CPS1", "inputs": [ { "node_id": "R-HSA-9959836", "reactome_id": "R-HSA-9959836", "label": "CPS1 gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-70544", "reactome_id": "R-HSA-70544", "label": "CPS1", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:30842655", "title": "p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis", "journal": "Nature", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30842655/" }, { "id": "PMID:17900240", "title": "Human ornithine decarboxylase paralogue (ODCp) is an antizyme inhibitor but not an arginine decarboxylase", "journal": "Biochem J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17900240/" }, { "id": "PMID:17407445", "title": "A structural insight into the inhibition of human and Leishmania donovani ornithine decarboxylases by 1-amino-oxy-3-aminopropane", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17407445/" }, { "id": "PMID:35477776", "title": "Polyamines in cancer: integrating organismal metabolism and antitumour immunity", "journal": "Nat Rev Cancer", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35477776/" } ], "x": 1251.3333333333333, "y": 3332.5555555555557, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9959881", "label": "OTC gene expression", "schema_class": "BlackBoxEvent", "domain_id": "nitrogen-one-carbon", "pathway_ids": [ "urea-cycle" ], "compartment": "cytoplasm · nucleoplasm", "compartment_ids": [ "GO:0005737", "GO:0005654" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9959881", "summary": "OTC gene expression is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects OTC gene to OTC precursor in cytoplasm · nucleoplasm.", "equation": "OTC gene → OTC precursor", "inputs": [ { "node_id": "R-HSA-9959831", "reactome_id": "R-HSA-9959831", "label": "OTC gene", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "nucleoplasm", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "outputs": [ { "node_id": "R-HSA-9956408", "reactome_id": "R-HSA-9956408", "label": "OTC precursor", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "EntityWithAccessionedSequence", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:30842655", "title": "p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis", "journal": "Nature", "year": 2019, "url": "https://pubmed.ncbi.nlm.nih.gov/30842655/" }, { "id": "PMID:17900240", "title": "Human ornithine decarboxylase paralogue (ODCp) is an antizyme inhibitor but not an arginine decarboxylase", "journal": "Biochem J", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/17900240/" }, { "id": "PMID:17407445", "title": "A structural insight into the inhibition of human and Leishmania donovani ornithine decarboxylases by 1-amino-oxy-3-aminopropane", "journal": "Biochem J", "year": 2007, "url": "https://pubmed.ncbi.nlm.nih.gov/17407445/" }, { "id": "PMID:35477776", "title": "Polyamines in cancer: integrating organismal metabolism and antitumour immunity", "journal": "Nat Rev Cancer", "year": 2022, "url": "https://pubmed.ncbi.nlm.nih.gov/35477776/" } ], "x": 1530, "y": 3332.5555555555557, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9960428", "label": "2AMAS transport from peroxisome to mitochondrial matrix", "schema_class": "BlackBoxEvent", "domain_id": "amino-acid-carbon", "pathway_ids": [ "lysine" ], "compartment": "peroxisomal matrix · mitochondrial matrix", "compartment_ids": [ "GO:0005782", "GO:0005759" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9960428", "summary": "2AMAS transport from peroxisome to mitochondrial matrix is a Reactome release 97 curated black-box event whose internal chemistry is not expanded by the source record. The source record connects 2AMAS to 2AMAS in peroxisomal matrix · mitochondrial matrix.", "equation": "2AMAS → 2AMAS", "inputs": [ { "node_id": "R-ALL-9960430", "reactome_id": "R-ALL-9960430", "label": "2AMAS", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58321", "formula": "C6H11NO3", "compartment": "peroxisomal matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113556", "reactome_id": "R-ALL-113556", "label": "2AMAS", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58321", "formula": "C6H11NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:24043460", "title": "Lysine metabolism in mammalian brain: an update on the importance of recent discoveries", "journal": "Amino Acids", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/24043460/" } ], "x": 6521.333333333333, "y": 2149.0416666666665, "lod": "pathway", "collapsed": true, "transport": true }, { "id": "R-HSA-9970023", "label": "AOC1 dimer oxidizes MeHist", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:78368" ], "source_url": "https://reactome.org/content/detail/R-HSA-9970023", "summary": "AOC1 dimer oxidizes MeHist is a Reactome release 97 curated reaction event. The source record connects H2O, MeHist, O2 to methylimidazole-aldehyde, H2O2, NH4+ in cytosol and cross-references RHEA:78368.", "equation": "H2O + MeHist + O2 → methylimidazole-aldehyde + H2O2 + NH4+", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-175991", "reactome_id": "R-ALL-175991", "label": "MeHist", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58600", "formula": "C6H12N3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29368", "reactome_id": "R-ALL-29368", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9970025", "reactome_id": "R-ALL-9970025", "label": "methylimidazole-aldehyde", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28104", "formula": "C6H8N2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29408", "reactome_id": "R-ALL-29408", "label": "H2O2", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16240", "formula": "H2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-31633", "reactome_id": "R-ALL-31633", "label": "NH4+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:28938", "formula": "H4N", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "histamine oxidase activity of AOC1 dimer:2xCu2+:4xCa2+ [plasma membrane]", "activity_id": "5696132", "ec_numbers": [ "1.4.3.22" ], "accessions": [] } ], "citations": [ { "id": "PMID:12072962", "title": "Human kidney diamine oxidase: heterologous expression, purification, and characterization", "journal": "J Biol Inorg Chem", "year": 2002, "url": "https://pubmed.ncbi.nlm.nih.gov/12072962/" } ], "x": 8018.666666666667, "y": 2105.4375, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9970035", "label": "Unknown ALDH oxidizes methylimidazolealdehyde", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "histidine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:16186" ], "source_url": "https://reactome.org/content/detail/R-HSA-9970035", "summary": "Unknown ALDH oxidizes methylimidazolealdehyde is a Reactome release 97 curated reaction event. The source record connects methylimidazole-aldehyde, H2O, NAD(P)+ to H+, NAD(P)H, methylimidazole-acetate in cytosol and cross-references RHEA:16186.", "equation": "methylimidazole-aldehyde + H2O + NAD(P)+ → 2 H+ + NAD(P)H + methylimidazole-acetate", "inputs": [ { "node_id": "R-ALL-9970025", "reactome_id": "R-ALL-9970025", "label": "methylimidazole-aldehyde", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:28104", "formula": "C6H8N2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428218", "reactome_id": "R-ALL-428218", "label": "NAD(P)+", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-428206", "reactome_id": "R-ALL-428206", "label": "NAD(P)H", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "cytosol", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-9970029", "reactome_id": "R-ALL-9970029", "label": "methylimidazole-acetate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:132918", "formula": "C6H7N2O2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of Unknown aldehyde dehydrogenase [cytosol]", "activity_id": "9970018", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:28694431", "title": "Key Role for the Organic Anion Transporters, OAT1 and OAT3, in the in vivo Handling of Uremic Toxins and Solutes", "journal": "Sci Rep", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28694431/" }, { "id": "PMID:10344773", "title": "Alcohol-histamine interactions", "journal": "Alcohol Alcohol", "year": 1999, "url": "https://pubmed.ncbi.nlm.nih.gov/10344773/" }, { "id": "PMID:1799975", "title": "Aldehyde dehydrogenase (EC 1.2.1.3): comparison of subcellular localization of the third isozyme that dehydrogenates gamma-aminobutyraldehyde in rat, guinea pig and human liver", "journal": "Comp Biochem Physiol B", "year": 1991, "url": "https://pubmed.ncbi.nlm.nih.gov/1799975/" } ], "x": 7461.333333333333, "y": 2142.8125, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971063", "label": "GLYAT-like proteins transfer glycine to phenylacetyl-CoA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:19870" ], "source_url": "https://reactome.org/content/detail/R-HSA-9971063", "summary": "GLYAT-like proteins transfer glycine to phenylacetyl-CoA is a Reactome release 97 curated reaction event. The source record connects Gly, phenylacetyl-CoA to CoA-SH, phenylacetyl-Gly, H+ in mitochondrial matrix and cross-references RHEA:19870.", "equation": "Gly + phenylacetyl-CoA → CoA-SH + phenylacetyl-Gly + H+", "inputs": [ { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-177137", "reactome_id": "R-ALL-177137", "label": "phenylacetyl-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57390", "formula": "C29H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971077", "reactome_id": "R-ALL-9971077", "label": "phenylacetyl-Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:60874", "formula": "C10H10NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycine N-benzoyltransferase activity of GLYAT-like proteins [mitochondrial matrix]", "activity_id": "159547", "ec_numbers": [ "2.3.1.71" ], "accessions": [] } ], "citations": [ { "id": "PMID:7802672", "title": "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver", "journal": "Biochem Biophys Res Commun", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7802672/" }, { "id": "PMID:22475485", "title": "Designation of enzyme activity of glycine-N-acyltransferase family genes and depression of glycine-N-acyltransferase in human hepatocellular carcinoma", "journal": "Biochem Biophys Res Commun", "year": 2012, "url": "https://pubmed.ncbi.nlm.nih.gov/22475485/" } ], "x": 7740, "y": 2867.8333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971094", "label": "SLC25A20 exports excess acyl-CAR", "schema_class": "Reaction", "domain_id": "fatty-acid-flow", "pathway_ids": [ "carnitine-shuttle", "fatty-acid-metabolism" ], "compartment": "mitochondrial inner membrane", "compartment_ids": [ "GO:0005743" ], "rhea_ids": [ "RHEA:49925" ], "source_url": "https://reactome.org/content/detail/R-HSA-9971094", "summary": "SLC25A20 exports excess acyl-CAR is a Reactome release 97 curated reaction event. The source record connects Acyl-CAR, CAR to Acyl-CAR, CAR in mitochondrial inner membrane and cross-references RHEA:49925.", "equation": "Acyl-CAR + CAR → Acyl-CAR + CAR", "inputs": [ { "node_id": "R-ALL-9971097", "reactome_id": "R-ALL-9971097", "label": "Acyl-CAR", "role": "input", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial matrix", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-549207", "reactome_id": "R-ALL-549207", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial intermembrane space", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9971099", "reactome_id": "R-ALL-9971099", "label": "Acyl-CAR", "role": "output", "stoichiometry": 1, "chebi_id": "", "formula": "", "compartment": "mitochondrial intermembrane space", "entity_kind": "DefinedSet", "currency": false }, { "node_id": "R-ALL-201023", "reactome_id": "R-ALL-201023", "label": "CAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carnitine:O-acyl-L-carnitine antiporter activity of SLC25A20 [mitochondrial inner membrane]", "activity_id": "200567", "ec_numbers": null, "accessions": [ "O43772" ] } ], "citations": [ { "id": "PMID:9654057", "title": "Carnitine palmitoyltransferase II specificity towards beta-oxidation intermediates--evidence for a reverse carnitine cycle in mitochondria", "journal": "Eur J Biochem", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9654057/" }, { "id": "PMID:9048718", "title": "Characterisation of carnitine palmitoyltransferases in patients with a carnitine palmitoyltransferase deficiency: implications for diagnosis and therapy", "journal": "J Neurol Neurosurg Psychiatry", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9048718/" } ], "x": 590, "y": 1691.421052631579, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971123", "label": "CRAT transfers ISB to CAR", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:44989" ], "source_url": "https://reactome.org/content/detail/R-HSA-9971123", "summary": "CRAT transfers ISB to CAR is a Reactome release 97 curated reaction event. The source record connects CAR, ISB-CoA to CoA-SH, ISB-CAR in mitochondrial matrix and cross-references RHEA:44989.", "equation": "CAR + ISB-CoA → CoA-SH + ISB-CAR", "inputs": [ { "node_id": "R-ALL-201023", "reactome_id": "R-ALL-201023", "label": "CAR", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16347", "formula": "C7H15NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-70851", "reactome_id": "R-ALL-70851", "label": "ISB-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57338", "formula": "C25H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971124", "reactome_id": "R-ALL-9971124", "label": "ISB-CAR", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:84838", "formula": "C11H21NO4", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "carnitine O-acyltransferase activity of CRAT [mitochondrial inner membrane]", "activity_id": "9971121", "ec_numbers": [ "2.3.1" ], "accessions": [ "P43155" ] } ], "citations": [ { "id": "PMID:23485643", "title": "Substrate specificity of human carnitine acetyltransferase: Implications for fatty acid and branched-chain amino acid metabolism", "journal": "Biochim Biophys Acta", "year": 2013, "url": "https://pubmed.ncbi.nlm.nih.gov/23485643/" } ], "x": 6521.333333333333, "y": 1657.5833333333333, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971246", "label": "Unknown CYP 450 omega-hydroxylates C10 fatty acid", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "smooth endoplasmic reticulum", "compartment_ids": [ "GO:0005790" ], "rhea_ids": [ "RHEA:75284" ], "source_url": "https://reactome.org/content/detail/R-HSA-9971246", "summary": "Unknown CYP 450 omega-hydroxylates C10 fatty acid is a Reactome release 97 curated reaction event. The source record connects O2, NADPH, DECA to H2O, NADP+, 10-OH-DECA in smooth endoplasmic reticulum and cross-references RHEA:75284.", "equation": "O2 + NADPH + DECA → H2O + NADP+ + 10-OH-DECA + H+", "inputs": [ { "node_id": "R-ALL-113534", "reactome_id": "R-ALL-113534", "label": "O2", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15379", "formula": "O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113601", "reactome_id": "R-ALL-113601", "label": "NADPH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:16474", "formula": "C21H30N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-422019", "reactome_id": "R-ALL-422019", "label": "DECA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:27689", "formula": "C10H19O2", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-113519", "reactome_id": "R-ALL-113519", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-113564", "reactome_id": "R-ALL-113564", "label": "NADP+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:18009", "formula": "C21H29N7O17P3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971242", "reactome_id": "R-ALL-9971242", "label": "10-OH-DECA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:11305", "formula": "C10H19O3", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-156540", "reactome_id": "R-ALL-156540", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "endoplasmic reticulum lumen", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "monooxygenase activity of Unknown Cytochrome P450-like monooxygenase [endoplasmic reticulum membrane]", "activity_id": "9971240", "ec_numbers": null, "accessions": [] } ], "citations": [ { "id": "PMID:8914854", "title": "Identification of CYP4A11 as the major lauric acid omega-hydroxylase in human liver microsomes", "journal": "Arch Biochem Biophys", "year": 1996, "url": "https://pubmed.ncbi.nlm.nih.gov/8914854/" }, { "id": "PMID:6634246", "title": "On the biologic origin of C6-C10-dicarboxylic and C6-C10-omega-1-hydroxy monocarboxylic acids in human and rat with acyl-CoA dehydrogenation deficiencies: in vitro studies on the omega- and omega-1-oxidation of medium-chain (C6-C12) fatty acids in human and rat liver", "journal": "Pediatr Res", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6634246/" } ], "x": 7461.333333333333, "y": 2829.6960784313724, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971258", "label": "ADH7 dehydrogenates 10-OH-DECA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:20881" ], "source_url": "https://reactome.org/content/detail/R-HSA-9971258", "summary": "ADH7 dehydrogenates 10-OH-DECA is a Reactome release 97 curated reaction event. The source record connects NAD+, 10-OH-DECA to 10-oxo-DECA, NADH, H+ in cytosol and cross-references RHEA:20881.", "equation": "NAD+ + 10-OH-DECA → 10-oxo-DECA + NADH + H+", "inputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971428", "reactome_id": "R-ALL-9971428", "label": "10-OH-DECA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:11305", "formula": "C10H19O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9971255", "reactome_id": "R-ALL-9971255", "label": "10-oxo-DECA", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:58022", "formula": "C10H17O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "omega-hydroxydecanoate dehydrogenase activity of ADH7:2Zn2+ dimer [cytosol]", "activity_id": "9971251", "ec_numbers": [ "1.1.1.66" ], "accessions": [] } ], "citations": [ { "id": "PMID:9600267", "title": "Retinoids, omega-hydroxyfatty acids and cytotoxic aldehydes as physiological substrates, and H2-receptor antagonists as pharmacological inhibitors, of human class IV alcohol dehydrogenase", "journal": "FEBS Lett", "year": 1998, "url": "https://pubmed.ncbi.nlm.nih.gov/9600267/" } ], "x": 8018.666666666667, "y": 2867.8333333333335, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971429", "label": "Unknown ALDH oxidizes 10-oxo-DECA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-one" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:49833" ], "source_url": "https://reactome.org/content/detail/R-HSA-9971429", "summary": "Unknown ALDH oxidizes 10-oxo-DECA is a Reactome release 97 curated reaction event. The source record connects H2O, 10-oxo-DECA, NAD+ to H+, sebacate, NADH in cytosol and cross-references RHEA:49833.", "equation": "H2O + 10-oxo-DECA + NAD+ → 2 H+ + sebacate + NADH", "inputs": [ { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971255", "reactome_id": "R-ALL-9971255", "label": "10-oxo-DECA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58022", "formula": "C10H17O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 2, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971434", "reactome_id": "R-ALL-9971434", "label": "sebacate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:76283", "formula": "C10H16O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "aldehyde dehydrogenase (NAD+) activity of Unknown aldehyde dehydrogenase [cytosol]", "activity_id": "9970018", "ec_numbers": [ "1.2.1.3" ], "accessions": [] } ], "citations": [ { "id": "PMID:28694431", "title": "Key Role for the Organic Anion Transporters, OAT1 and OAT3, in the in vivo Handling of Uremic Toxins and Solutes", "journal": "Sci Rep", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/28694431/" }, { "id": "PMID:9133646", "title": "Human liver fatty aldehyde dehydrogenase: microsomal localization, purification, and biochemical characterization", "journal": "Biochim. Biophys. Acta", "year": 1997, "url": "https://pubmed.ncbi.nlm.nih.gov/9133646/" } ], "x": 7461.333333333333, "y": 2860.205882352941, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971565", "label": "Unknown enzyme hydrogenates HPP", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "phenylalanine-tyrosine" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:34557" ], "source_url": "https://reactome.org/content/detail/R-HSA-9971565", "summary": "Unknown enzyme hydrogenates HPP is a Reactome release 97 curated reaction event. The source record connects H+, HPP, NADH to NAD+, p-hydroxyphenyllactate in cytosol and cross-references RHEA:34557.", "equation": "H+ + HPP + NADH → NAD+ + p-hydroxyphenyllactate", "inputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-31365", "reactome_id": "R-ALL-31365", "label": "HPP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36242", "formula": "C9H7O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-73473", "reactome_id": "R-ALL-73473", "label": "NADH", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57945", "formula": "C21H27N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "outputs": [ { "node_id": "R-ALL-29360", "reactome_id": "R-ALL-29360", "label": "NAD+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57540", "formula": "C21H26N7O14P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971564", "reactome_id": "R-ALL-9971564", "label": "p-hydroxyphenyllactate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:10980", "formula": "C9H9O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "L-2-hydroxycarboxylate dehydrogenase (NAD+) activity of Unknown enzyme [cytosol]", "activity_id": "9971559", "ec_numbers": [ "1.1.1.337" ], "accessions": [] } ], "citations": [ { "id": "PMID:32271169", "title": "Gut-derived uremic toxin handling in vivo requires OAT-mediated tubular secretion in chronic kidney disease", "journal": "JCI Insight", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32271169/" }, { "id": "PMID:2449162", "title": "The reduction of aromatic alpha-keto acids by cytoplasmic malate dehydrogenase and lactate dehydrogenase", "journal": "Biochem Genet", "year": 1987, "url": "https://pubmed.ncbi.nlm.nih.gov/2449162/" }, { "id": "PMID:11370259", "title": "Tyrosinemia: a review", "journal": "Pediatr Dev Pathol", "year": 2001, "url": "https://pubmed.ncbi.nlm.nih.gov/11370259/" } ], "x": 7078.666666666667, "y": 1877.15, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971609", "label": "2AMA spontaneously forms picolinate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9971609", "summary": "2AMA spontaneously forms picolinate is a Reactome release 97 curated reaction event. The source record connects 2AMA to H+, H2O, picolinate in cytosol.", "equation": "2AMA → H+ + H2O + picolinate", "inputs": [ { "node_id": "R-ALL-35181", "reactome_id": "R-ALL-35181", "label": "2AMA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:77634", "formula": "C6H7NO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971608", "reactome_id": "R-ALL-9971608", "label": "picolinate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:38184", "formula": "C6H4NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [], "citations": [ { "id": "PMID:22084582", "title": "Effects of Kynurenine Pathway Metabolites on Intracellular NAD Synthesis and Cell Death in Human Primary Astrocytes and Neurons", "journal": "Int J Tryptophan Res", "year": 2009, "url": "https://pubmed.ncbi.nlm.nih.gov/22084582/" } ], "x": 8018.666666666667, "y": 1909.5416666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971621", "label": "GLYAT transfers ISB to glycine", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "branched-chain" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:19870" ], "source_url": "https://reactome.org/content/detail/R-HSA-9971621", "summary": "GLYAT transfers ISB to glycine is a Reactome release 97 curated reaction event. The source record connects ISB-CoA, Gly to CoA-SH, ISB-Gly, H+ in mitochondrial matrix and cross-references RHEA:19870.", "equation": "ISB-CoA + Gly → CoA-SH + ISB-Gly + H+", "inputs": [ { "node_id": "R-ALL-70851", "reactome_id": "R-ALL-70851", "label": "ISB-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57338", "formula": "C25H38N7O17P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9971623", "reactome_id": "R-ALL-9971623", "label": "ISB-Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:133610", "formula": "C6H10NO3", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycine N-acyltransferase activity of GLYAT [mitochondrial matrix]", "activity_id": "9971619", "ec_numbers": [ "2.3.1.13" ], "accessions": [ "Q6IB77" ] } ], "citations": [ { "id": "PMID:3707752", "title": "Acyl-CoA: glycine N-acyltransferase: in vitro studies on the glycine conjugation of straight- and branched-chained acyl-CoA esters in human liver", "journal": "Biochem Med Metab Biol", "year": 1986, "url": "https://pubmed.ncbi.nlm.nih.gov/3707752/" } ], "x": 7078.666666666667, "y": 1640.9722222222222, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9971629", "label": "Unknown NAT N-acetylates kynurenine", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [], "source_url": "https://reactome.org/content/detail/R-HSA-9971629", "summary": "Unknown NAT N-acetylates kynurenine is a Reactome release 97 curated reaction event. The source record connects Ac-CoA, L-KYN to N-acetylkynurenine, CoA-SH, H+ in cytosol.", "equation": "Ac-CoA + L-KYN → N-acetylkynurenine + CoA-SH + H+", "inputs": [ { "node_id": "R-ALL-76183", "reactome_id": "R-ALL-76183", "label": "Ac-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57288", "formula": "C23H34N7O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29970", "reactome_id": "R-ALL-29970", "label": "L-KYN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57959", "formula": "C10H12N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9971631", "reactome_id": "R-ALL-9971631", "label": "N-acetylkynurenine", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:746924", "formula": "C12H13N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-76194", "reactome_id": "R-ALL-76194", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-70106", "reactome_id": "R-ALL-70106", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "N-acetyltransferase activity of unknown NAT [cytosol]", "activity_id": "9971630", "ec_numbers": [ "2.3.1.5" ], "accessions": [] } ], "citations": [ { "id": "PMID:32271169", "title": "Gut-derived uremic toxin handling in vivo requires OAT-mediated tubular secretion in chronic kidney disease", "journal": "JCI Insight", "year": 2020, "url": "https://pubmed.ncbi.nlm.nih.gov/32271169/" } ], "x": 7740, "y": 1909.5416666666667, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9972889", "label": "AADAT transaminates 3HKYN to xanthurenate", "schema_class": "Reaction", "domain_id": "amino-acid-carbon", "pathway_ids": [ "tryptophan" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:65901" ], "source_url": "https://reactome.org/content/detail/R-HSA-9972889", "summary": "AADAT transaminates 3HKYN to xanthurenate is a Reactome release 97 curated reaction event. The source record connects 3HKYN, glyoxylate to xanthurenate, Gly, H2O in cytosol and cross-references RHEA:65901.", "equation": "3HKYN + glyoxylate → xanthurenate + Gly + H2O", "inputs": [ { "node_id": "R-ALL-34351", "reactome_id": "R-ALL-34351", "label": "3HKYN", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58125", "formula": "C10H12N2O4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-5367039", "reactome_id": "R-ALL-5367039", "label": "glyoxylate", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:36655", "formula": "C2HO3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-9972885", "reactome_id": "R-ALL-9972885", "label": "xanthurenate", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:71201", "formula": "C10H6NO4", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29424", "reactome_id": "R-ALL-29424", "label": "Gly", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-29356", "reactome_id": "R-ALL-29356", "label": "H2O", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15377", "formula": "H2O", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "transaminase activity of AADAT dimer [cytosol]", "activity_id": "9972882", "ec_numbers": [ "2.6.1" ], "accessions": [] } ], "citations": [ { "id": "PMID:18620547", "title": "Substrate specificity and structure of human aminoadipate aminotransferase/kynurenine aminotransferase II", "journal": "Biosci Rep", "year": 2008, "url": "https://pubmed.ncbi.nlm.nih.gov/18620547/" }, { "id": "PMID:29031603", "title": "Xanthurenic Acid Formation from 3-Hydroxykynurenine in the Mammalian Brain: Neurochemical Characterization and Physiological Effects", "journal": "Neuroscience", "year": 2017, "url": "https://pubmed.ncbi.nlm.nih.gov/29031603/" }, { "id": "PMID:15880762", "title": "Demonstration of kynurenine aminotransferases I and II and characterization of kynurenic acid synthesis in cultured cerebral cortical neurons", "journal": "J Neurosci Res", "year": 2005, "url": "https://pubmed.ncbi.nlm.nih.gov/15880762/" } ], "x": 8018.666666666667, "y": 1884.625, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-9972916", "label": "GLYAT-like proteins transfer glycine to aminobenzoyl-CoA", "schema_class": "Reaction", "domain_id": "heme-oxidations", "pathway_ids": [ "biological-oxidations", "phase-two" ], "compartment": "mitochondrial matrix", "compartment_ids": [ "GO:0005759" ], "rhea_ids": [ "RHEA:19870" ], "source_url": "https://reactome.org/content/detail/R-HSA-9972916", "summary": "GLYAT-like proteins transfer glycine to aminobenzoyl-CoA is a Reactome release 97 curated reaction event. The source record connects Gly, PABA-CoA to CoA-SH, PAH, H+ in mitochondrial matrix and cross-references RHEA:19870.", "equation": "Gly + PABA-CoA → CoA-SH + PAH + H+", "inputs": [ { "node_id": "R-ALL-159549", "reactome_id": "R-ALL-159549", "label": "Gly", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:57305", "formula": "C2H5NO2", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-9972915", "reactome_id": "R-ALL-9972915", "label": "PABA-CoA", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:188497", "formula": "C28H37N8O17P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29374", "reactome_id": "R-ALL-29374", "label": "CoA-SH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:57287", "formula": "C21H32N7O16P3S", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-9972914", "reactome_id": "R-ALL-9972914", "label": "PAH", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:64703", "formula": "C9H9N2O3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false }, { "node_id": "R-ALL-113529", "reactome_id": "R-ALL-113529", "label": "H+", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:15378", "formula": "H", "compartment": "mitochondrial matrix", "entity_kind": "SimpleEntity", "currency": true } ], "catalysts": [ { "label": "glycine N-benzoyltransferase activity of GLYAT-like proteins [mitochondrial matrix]", "activity_id": "159547", "ec_numbers": [ "2.3.1.71" ], "accessions": [] } ], "citations": [ { "id": "PMID:7802672", "title": "Purification to homogeneity of mitochondrial acyl coa:glycine n-acyltransferase from human liver", "journal": "Biochem Biophys Res Commun", "year": 1994, "url": "https://pubmed.ncbi.nlm.nih.gov/7802672/" }, { "id": "PMID:8582053", "title": "Glycine conjugation of para-aminobenzoic acid (PABA): a quantitative test of liver function", "journal": "Clin Biochem", "year": 1995, "url": "https://pubmed.ncbi.nlm.nih.gov/8582053/" } ], "x": 7461.333333333333, "y": 2875.4607843137255, "lod": "pathway", "collapsed": false, "transport": false }, { "id": "R-HSA-997381", "label": "TDPK phosphorylates ThDP to ThTP", "schema_class": "Reaction", "domain_id": "vitamins-cofactors", "pathway_ids": [ "water-soluble-vitamins" ], "compartment": "cytosol", "compartment_ids": [ "GO:0005829" ], "rhea_ids": [ "RHEA:11241" ], "source_url": "https://reactome.org/content/detail/R-HSA-997381", "summary": "TDPK phosphorylates ThDP to ThTP is a Reactome release 97 curated reaction event. The source record connects ATP, ThDP to ADP, ThTP in cytosol and cross-references RHEA:11241.", "equation": "ATP + ThDP → ADP + ThTP", "inputs": [ { "node_id": "R-ALL-113592", "reactome_id": "R-ALL-113592", "label": "ATP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:30616", "formula": "C10H12N5O13P3", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-29480", "reactome_id": "R-ALL-29480", "label": "ThDP", "role": "input", "stoichiometry": 1, "chebi_id": "CHEBI:58937", "formula": "C12H16N4O7P2S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "outputs": [ { "node_id": "R-ALL-29370", "reactome_id": "R-ALL-29370", "label": "ADP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:456216", "formula": "C10H12N5O10P2", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": true }, { "node_id": "R-ALL-964965", "reactome_id": "R-ALL-964965", "label": "ThTP", "role": "output", "stoichiometry": 1, "chebi_id": "CHEBI:9534", "formula": "C12H20N4O10P3S", "compartment": "cytosol", "entity_kind": "SimpleEntity", "currency": false } ], "catalysts": [ { "label": "thiamine-diphosphate kinase activity of TDPK [cytosol]", "activity_id": "997383", "ec_numbers": [ "2.7.4.15" ], "accessions": [] } ], "citations": [ { "id": "PMID:6311826", "title": "Enzyme system involved in the synthesis of thiamin triphosphate. I. Purification and characterization of protein-bound thiamin diphosphate: ATP phosphoryltransferase", "journal": "J Biol Chem", "year": 1983, "url": "https://pubmed.ncbi.nlm.nih.gov/6311826/" } ], "x": 5670, "y": 3002.6184210526317, "lod": "pathway", "collapsed": false, "transport": false } ], "connections": [], "learning": [ { "id": "carbohydrate-core", "title": "Carbohydrate core", "kicker": "CARBOHYDRATES", "summary": "Routes that capture, store, release and repartition six-carbon sugars, with glycolysis as a central exchange rather than an isolated tube.", "paragraphs": [ "Carbohydrate metabolism is best read as a set of reversible exchanges interrupted by strategically directed transformations. Glucose 6-phosphate sits near storage, oxidation and biosynthetic branches, so its meaning depends on which enzyme, compartment and energetic state are in view.", "The pentose phosphate pathway is not simply an alternate route to glycolysis. Its oxidative arm changes the NADPH pool, while its non-oxidative carbon rearrangements connect sugars of different lengths to ribose and glycolytic intermediates." ], "key_ideas": [ "Storage, oxidation and biosynthesis meet at sugar phosphates.", "Carbon rearrangement and redox output are separate dimensions.", "Compartment and enzyme identity determine direction." ], "pathway_ids": [ "glycolysis", "gluconeogenesis", "pentose-phosphate", "glycogen" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-70171", "url": "https://reactome.org/content/detail/R-HSA-70171" }, { "source": "Reactome pathway", "id": "R-HSA-70263", "url": "https://reactome.org/content/detail/R-HSA-70263" }, { "source": "Reactome pathway", "id": "R-HSA-71336", "url": "https://reactome.org/content/detail/R-HSA-71336" }, { "source": "Reactome pathway", "id": "R-HSA-8982491", "url": "https://reactome.org/content/detail/R-HSA-8982491" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "sugar-branches-glycans", "title": "Sugar branches \u0026 glycans", "kicker": "SUGAR BRANCHES", "summary": "Monosaccharide entry routes and the activated-sugar chemistry used to construct, remodel and recover extracellular-matrix carbohydrates.", "paragraphs": [ "Fructose and galactose do not enter central metabolism through one universal gate. Their first reactions depend on tissue enzyme expression and create distinct phosphorylated intermediates before carbon reaches the glucose-phosphate or triose-phosphate network.", "Glycosaminoglycans are not fuel polymers. Their repeating disaccharides are assembled, modified and sulfated through activated donors in the secretory system, then degraded through ordered lysosomal steps. Missing one step can block turnover even when neighboring hydrolases remain active.", "The map keeps monosaccharide catabolism and glycan assembly in one territory to show shared activated-sugar chemistry without implying that extracellular-matrix chains feed directly into glycolysis." ], "key_ideas": [ "Different dietary sugars have distinct entry chemistry.", "Activated sugars support biosynthesis as well as catabolism.", "Lysosomal glycan turnover is ordered and compartment-specific." ], "pathway_ids": [ "fructose", "galactose", "glycosaminoglycans", "oligosaccharide-recovery" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-5652084", "url": "https://reactome.org/content/detail/R-HSA-5652084" }, { "source": "Reactome pathway", "id": "R-HSA-70370", "url": "https://reactome.org/content/detail/R-HSA-70370" }, { "source": "Reactome pathway", "id": "R-HSA-1630316", "url": "https://reactome.org/content/detail/R-HSA-1630316" }, { "source": "Reactome pathway", "id": "R-HSA-8853383", "url": "https://reactome.org/content/detail/R-HSA-8853383" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "central-energy", "title": "Central energy conversion", "kicker": "ENERGY", "summary": "The mitochondrial and cytosolic interfaces that oxidize carbon, transfer reducing equivalents and couple electron movement to ATP formation.", "paragraphs": [ "The citric acid cycle is simultaneously an oxidative sequence and an exchange hub. Intermediates can leave for biosynthesis or enter from other routes, which is why anaplerotic and cataplerotic reactions belong beside the ring rather than in a footnote.", "Respiratory electron transport and ATP formation are coupled through a membrane gradient, not through a direct chemical handoff from NADH to ATP. The compartment boundary is therefore a functional component of the system." ], "key_ideas": [ "The TCA cycle is an exchange hub.", "A membrane gradient couples electron transport to ATP synthesis.", "Displayed direction is not a measured rate." ], "pathway_ids": [ "pyruvate", "tca", "electron-transport", "chemiosmosis" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-70268", "url": "https://reactome.org/content/detail/R-HSA-70268" }, { "source": "Reactome pathway", "id": "R-HSA-71403", "url": "https://reactome.org/content/detail/R-HSA-71403" }, { "source": "Reactome pathway", "id": "R-HSA-611105", "url": "https://reactome.org/content/detail/R-HSA-611105" }, { "source": "Reactome pathway", "id": "R-HSA-163210", "url": "https://reactome.org/content/detail/R-HSA-163210" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "metabolic-integration", "title": "Metabolic integration", "kicker": "INTEGRATION", "summary": "Shuttles, exchange reactions and alternate fuels that connect otherwise separated compartments and pathway neighborhoods.", "paragraphs": [ "Metabolic integration is dominated by coupled systems. The malate–aspartate shuttle combines dehydrogenases, aminotransferases and antiporters so that cytosolic reducing equivalents influence the matrix NADH pool without NADH itself crossing the inner membrane.", "Ketone bodies package acetyl-derived carbon into water-soluble molecules. Their production and use occur in different physiological contexts and enzyme complements; a shared bloodstream does not make every tissue a net producer.", "Carbon dioxide hydration and inositol-phosphate transport illustrate two different integration problems: rapid acid–base interconversion in defined compartments, and movement of highly phosphorylated signals among cytosol, nucleus and endoplasmic reticulum." ], "key_ideas": [ "Shuttles transfer equivalents through coupled reactions.", "Ketone production and utilization are not tissue-equivalent.", "Transport events are part of pathway chemistry." ], "pathway_ids": [ "malate-aspartate-shuttle", "ketone-bodies", "carbon-dioxide", "inositol-phosphate" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-9856872", "url": "https://reactome.org/content/detail/R-HSA-9856872" }, { "source": "Reactome pathway", "id": "R-HSA-74182", "url": "https://reactome.org/content/detail/R-HSA-74182" }, { "source": "Reactome pathway", "id": "R-HSA-1475029", "url": "https://reactome.org/content/detail/R-HSA-1475029" }, { "source": "Reactome pathway", "id": "R-HSA-1483249", "url": "https://reactome.org/content/detail/R-HSA-1483249" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "fatty-acid-flow", "title": "Fatty-acid flow", "kicker": "FATTY ACIDS", "summary": "Activation, mitochondrial entry, synthesis and staged oxidation of fatty-acid carbon.", "paragraphs": [ "A fatty acid must normally be activated as an acyl-CoA before many downstream reactions. For long chains, the carnitine shuttle transfers the acyl group while CoA pools remain compartmentalized.", "Each beta-oxidation turn contains a repeated chemical motif, but chain length, unsaturation and organelle context introduce distinct supporting enzymes. A repeated visual rhythm should not erase those branches." ], "key_ideas": [ "Activation precedes most acyl chemistry.", "Carnitine transfers acyl groups across mitochondrial membranes.", "Unsaturation and chain length create real branches." ], "pathway_ids": [ "fatty-acid-metabolism", "fatty-acyl-coa", "carnitine-shuttle", "beta-oxidation", "very-long-chain-acyl-coa", "peroxisomal-lipids" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-8978868", "url": "https://reactome.org/content/detail/R-HSA-8978868" }, { "source": "Reactome pathway", "id": "R-HSA-75105", "url": "https://reactome.org/content/detail/R-HSA-75105" }, { "source": "Reactome pathway", "id": "R-HSA-200425", "url": "https://reactome.org/content/detail/R-HSA-200425" }, { "source": "Reactome pathway", "id": "R-HSA-77289", "url": "https://reactome.org/content/detail/R-HSA-77289" }, { "source": "Reactome pathway", "id": "R-HSA-75876", "url": "https://reactome.org/content/detail/R-HSA-75876" }, { "source": "Reactome pathway", "id": "R-HSA-390918", "url": "https://reactome.org/content/detail/R-HSA-390918" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "complex-lipids", "title": "Complex lipids", "kicker": "COMPLEX LIPIDS", "summary": "Membrane, storage and signaling-lipid chemistry spanning the endoplasmic reticulum, Golgi, mitochondria, lysosome and plasma membrane.", "paragraphs": [ "Complex lipid metabolism is organized around membranes. Glycerophospholipids, sphingolipids and sterols are synthesized and remodeled on particular membrane faces, then moved by vesicles, transfer proteins or transporters. The destination membrane can be as important as the headgroup name.", "Triacylglycerol stores three fatty acyl groups on glycerol, whereas phospholipids and sphingolipids contribute to bilayer structure and signaling. Hydrolysis can connect these pools, but the enzymes and products are chemically specific.", "Cholesterol biosynthesis proceeds through isoprenoid and sterol intermediates before the final double-bond and side-chain transformations. Cholesterol then becomes a membrane component and a precursor for bile acids, steroid hormones and vitamin-D-related chemistry." ], "key_ideas": [ "Membrane location is part of lipid metabolism.", "Storage and structural lipids solve different cellular problems.", "Cholesterol connects membranes to several biosynthetic branches." ], "pathway_ids": [ "phospholipids", "sphingolipids", "cholesterol", "triglycerides" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-1483257", "url": "https://reactome.org/content/detail/R-HSA-1483257" }, { "source": "Reactome pathway", "id": "R-HSA-428157", "url": "https://reactome.org/content/detail/R-HSA-428157" }, { "source": "Reactome pathway", "id": "R-HSA-191273", "url": "https://reactome.org/content/detail/R-HSA-191273" }, { "source": "Reactome pathway", "id": "R-HSA-8979227", "url": "https://reactome.org/content/detail/R-HSA-8979227" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "lipid-mediators", "title": "Lipid mediators \u0026 sterols", "kicker": "LIPID MEDIATORS", "summary": "Sterol-derived hormones, bile acids and oxygenated polyunsaturated-fatty-acid mediators.", "paragraphs": [ "Lipid mediators are often made on demand from membrane-derived precursors. Arachidonate can be oxygenated through cyclooxygenase, lipoxygenase and cytochrome-P450 branches, producing families with different receptors and lifetimes.", "Specialized pro-resolving mediators arise through staged oxygenation of omega-3 and related polyunsaturated fatty acids. Their pathway placement describes biosynthetic chemistry; it does not by itself establish concentration, cell source or effect in a specific disease state.", "Steroid and bile-acid pathways begin with cholesterol but diverge through different organelles, tissues and conjugation steps. The map uses shared ancestry as a junction while preserving each reaction sequence." ], "key_ideas": [ "On-demand synthesis begins from defined lipid precursors.", "Oxygenase branches create distinct mediator families.", "Pathway topology alone does not establish a biological effect." ], "pathway_ids": [ "steroids", "bile-acids", "arachidonate", "proresolving-mediators" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-8957322", "url": "https://reactome.org/content/detail/R-HSA-8957322" }, { "source": "Reactome pathway", "id": "R-HSA-194068", "url": "https://reactome.org/content/detail/R-HSA-194068" }, { "source": "Reactome pathway", "id": "R-HSA-2142753", "url": "https://reactome.org/content/detail/R-HSA-2142753" }, { "source": "Reactome pathway", "id": "R-HSA-9018678", "url": "https://reactome.org/content/detail/R-HSA-9018678" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "amino-acid-carbon", "title": "Amino-acid carbon", "kicker": "AMINO ACIDS", "summary": "Amino-acid-specific transformations that connect carbon skeletons to central metabolism while preserving nitrogen handling as a separate question.", "paragraphs": [ "Amino-acid catabolism begins by redistributing or removing nitrogen, then channels the remaining carbon skeleton into a smaller set of metabolic entry points. Those products include pyruvate, acetyl-CoA, acetoacetate, 2-oxoglutarate, succinyl-CoA, fumarate and oxaloacetate.", "Branched-chain amino acids share an initial transamination and oxidative-decarboxylation logic but then diverge into chain-specific reactions. Phenylalanine and tyrosine retain aromatic chemistry much longer, while tryptophan follows the chemically diverse kynurenine route.", "The familiar labels glucogenic and ketogenic summarize possible carbon destinations; they do not specify a measured whole-body flux. Reaction-level detail is needed to see where cofactors, organelles and irreversible steps constrain each route." ], "key_ideas": [ "Nitrogen handling precedes many carbon-skeleton routes.", "Several amino acids converge on a small set of central intermediates.", "Glucogenic and ketogenic labels summarize destinations, not flux." ], "pathway_ids": [ "branched-chain", "glutamate-glutamine", "phenylalanine-tyrosine", "tryptophan", "lysine", "histidine", "proline", "threonine" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-70895", "url": "https://reactome.org/content/detail/R-HSA-70895" }, { "source": "Reactome pathway", "id": "R-HSA-8964539", "url": "https://reactome.org/content/detail/R-HSA-8964539" }, { "source": "Reactome pathway", "id": "R-HSA-8963691", "url": "https://reactome.org/content/detail/R-HSA-8963691" }, { "source": "Reactome pathway", "id": "R-HSA-71240", "url": "https://reactome.org/content/detail/R-HSA-71240" }, { "source": "Reactome pathway", "id": "R-HSA-71064", "url": "https://reactome.org/content/detail/R-HSA-71064" }, { "source": "Reactome pathway", "id": "R-HSA-70921", "url": "https://reactome.org/content/detail/R-HSA-70921" }, { "source": "Reactome pathway", "id": "R-HSA-70688", "url": "https://reactome.org/content/detail/R-HSA-70688" }, { "source": "Reactome pathway", "id": "R-HSA-8849175", "url": "https://reactome.org/content/detail/R-HSA-8849175" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "nitrogen-one-carbon", "title": "Nitrogen, one-carbon \u0026 thiols", "kicker": "NITROGEN + C1", "summary": "Nitrogen disposal, one-carbon transfer, sulfur chemistry and redox-buffer synthesis at the amino-acid–nucleotide interface.", "paragraphs": [ "Carbon and nitrogen move together through amino-acid reactions but are disposed of through different networks. Transamination can redistribute amino groups without itself removing nitrogen from the body; the urea cycle supplies the dedicated disposal route.", "Tetrahydrofolate derivatives carry one-carbon units at several oxidation states. Their reactions connect serine and glycine chemistry to nucleotide construction and methyl-group metabolism." ], "key_ideas": [ "Transamination redistributes nitrogen; it does not dispose of it.", "THF carries one-carbon units at several oxidation states.", "Glutathione couples redox buffering to conjugation chemistry." ], "pathway_ids": [ "serine", "sulfur-amino-acids", "glycine-glyoxylate", "urea-cycle", "glutathione" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-977347", "url": "https://reactome.org/content/detail/R-HSA-977347" }, { "source": "Reactome pathway", "id": "R-HSA-1614635", "url": "https://reactome.org/content/detail/R-HSA-1614635" }, { "source": "Reactome pathway", "id": "R-HSA-389661", "url": "https://reactome.org/content/detail/R-HSA-389661" }, { "source": "Reactome pathway", "id": "R-HSA-70635", "url": "https://reactome.org/content/detail/R-HSA-70635" }, { "source": "Reactome pathway", "id": "R-HSA-174403", "url": "https://reactome.org/content/detail/R-HSA-174403" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "nucleotides", "title": "Nucleotide economy", "kicker": "NUCLEOTIDES", "summary": "Construction, interconversion, salvage and breakdown of purine and pyrimidine nucleotides.", "paragraphs": [ "Purine and pyrimidine nucleotides are built by different architectural strategies. Purine atoms are assembled stepwise on a ribose-phosphate scaffold, whereas a pyrimidine ring is largely constructed before attachment to ribose phosphate.", "Salvage pathways recover bases and nucleosides at lower synthetic cost, while catabolic pathways dephosphorylate, deaminate, cleave and oxidize them. The balance among synthesis, salvage and degradation depends on cell context and substrate availability.", "Nucleoside diphosphate kinases and related phosphate-transfer reactions connect nucleotide pools. The map keeps individual base identities visible rather than treating ATP as a generic synonym for every nucleoside triphosphate." ], "key_ideas": [ "De novo synthesis and salvage solve different supply problems.", "Purine and pyrimidine rings follow different construction logic.", "Nucleotide pools are continually interconverted." ], "pathway_ids": [ "nucleotide-biosynthesis", "nucleotide-salvage", "nucleotide-catabolism", "nucleotide-interconversion", "purine-catabolism", "pyrimidine-catabolism", "pyrimidine-salvage" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-8956320", "url": "https://reactome.org/content/detail/R-HSA-8956320" }, { "source": "Reactome pathway", "id": "R-HSA-8956321", "url": "https://reactome.org/content/detail/R-HSA-8956321" }, { "source": "Reactome pathway", "id": "R-HSA-8956319", "url": "https://reactome.org/content/detail/R-HSA-8956319" }, { "source": "Reactome pathway", "id": "R-HSA-499943", "url": "https://reactome.org/content/detail/R-HSA-499943" }, { "source": "Reactome pathway", "id": "R-HSA-74259", "url": "https://reactome.org/content/detail/R-HSA-74259" }, { "source": "Reactome pathway", "id": "R-HSA-73621", "url": "https://reactome.org/content/detail/R-HSA-73621" }, { "source": "Reactome pathway", "id": "R-HSA-73614", "url": "https://reactome.org/content/detail/R-HSA-73614" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "vitamins-cofactors", "title": "Vitamins \u0026 cofactors", "kicker": "COFACTORS", "summary": "Uptake, activation, transport and assembly of vitamin-derived and inorganic cofactors used throughout the network.", "paragraphs": [ "Vitamins become metabolically useful only after transport and chemical activation. The resulting cofactors participate in reaction classes—acyl transfer, redox, carboxylation, rearrangement or one-carbon transfer—rather than acting as generic energy ingredients.", "A cofactor lens exposes repeated chemistry across distant pathways. It also helps distinguish a nutrient precursor from the active molecular form that appears in an enzyme reaction." ], "key_ideas": [ "The active cofactor is not always the dietary molecule.", "Cofactors reveal recurring reaction chemistry.", "Transport and compartmental activation matter." ], "pathway_ids": [ "folate-pterines", "water-soluble-vitamins", "cofactor-metabolism", "iron-sulfur" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-196757", "url": "https://reactome.org/content/detail/R-HSA-196757" }, { "source": "Reactome pathway", "id": "R-HSA-196849", "url": "https://reactome.org/content/detail/R-HSA-196849" }, { "source": "Reactome pathway", "id": "R-HSA-8978934", "url": "https://reactome.org/content/detail/R-HSA-8978934" }, { "source": "Reactome pathway", "id": "R-HSA-1362409", "url": "https://reactome.org/content/detail/R-HSA-1362409" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "heme-oxidations", "title": "Heme \u0026 biological oxidations", "kicker": "HEME + OXIDATION", "summary": "Porphyrin synthesis and turnover alongside enzyme systems that functionalize and conjugate endogenous or environmental compounds.", "paragraphs": [ "Heme synthesis alternates between mitochondrial and cytosolic reactions, beginning with aminolevulinate formation and ending with iron insertion into protoporphyrin IX. Its geography makes transport of intermediates part of the pathway logic.", "Heme degradation opens the porphyrin ring and produces biliverdin, bilirubin and iron-handling consequences. Synthesis and breakdown are therefore connected by material identity but controlled through different enzyme systems.", "Phase-I transformations introduce or expose functional groups through oxidation, reduction or hydrolysis. Phase-II enzymes transfer polar groups such as glucuronate, sulfate or glutathione. These labels organize reaction types; they are not a guarantee that Phase I always precedes Phase II for a given compound." ], "key_ideas": [ "Heme synthesis crosses mitochondrial and cytosolic space.", "Functionalization and conjugation are reaction classes, not a mandatory sequence.", "Porphyrin turnover links ring chemistry to iron handling." ], "pathway_ids": [ "porphyrins", "biological-oxidations", "phase-one", "phase-two" ], "source_links": [ { "source": "Reactome metabolism hierarchy", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Reactome pathway", "id": "R-HSA-189445", "url": "https://reactome.org/content/detail/R-HSA-189445" }, { "source": "Reactome pathway", "id": "R-HSA-211859", "url": "https://reactome.org/content/detail/R-HSA-211859" }, { "source": "Reactome pathway", "id": "R-HSA-211945", "url": "https://reactome.org/content/detail/R-HSA-211945" }, { "source": "Reactome pathway", "id": "R-HSA-156580", "url": "https://reactome.org/content/detail/R-HSA-156580" } ], "further_links": [ { "source": "Rhea reaction knowledgebase", "id": "Rhea", "url": "https://www.rhea-db.org/" }, { "source": "ChEBI chemical ontology", "id": "ChEBI", "url": "https://www.ebi.ac.uk/chebi/" } ] }, { "id": "reading-the-field", "title": "How to read a metabolic atlas", "kicker": "MAP LITERACY", "summary": "A metabolic map is a projection of a reaction hypergraph: compounds participate in events, and events—not decorative line crossings—create biochemical connections.", "paragraphs": [ "A conventional arrow between two metabolite names can hide additional substrates, products and cofactors. The release model represents each reaction as its own node, then attaches every admitted input and output with a role and stoichiometric coefficient. This prevents a many-participant reaction from being mistaken for several independent pairwise conversions.", "The overview level organizes pathway territories; the pathway level exposes reaction spines and primary compounds; the reaction level reveals full participants, catalysts, compartments and citations. Moving between these levels changes visual density, not the underlying biological assertion.", "Small, frequently reused compounds can create nonsensical graph shortcuts. ATP, water, protons, nicotinamide cofactors and free CoA remain visible in reaction detail but are excluded as intermediate waypoints by the default route finder. A route therefore follows source-defined reaction direction without turning every ATP-using reaction into a neighbor.", "A pulsing route encodes ordered traversal only. Its speed is intentionally uniform and carries no information about flux, concentration, thermodynamic force or tissue abundance." ], "key_ideas": [ "Reactions are hypergraph nodes.", "Semantic zoom changes detail, not evidence.", "Currency compounds stay visible but do not create route shortcuts.", "Animated traversal is not measured flux." ], "pathway_ids": null, "source_links": [ { "source": "Reactome", "id": "R-HSA-1430728", "url": "https://reactome.org/content/detail/R-HSA-1430728" }, { "source": "Rhea", "id": "Rhea", "url": "https://www.rhea-db.org/" } ], "further_links": null }, { "id": "compartment-logic", "title": "Compartments are part of identity", "kicker": "CELLULAR GEOGRAPHY", "summary": "The same chemical name in cytosol, matrix, membrane or organelle lumen represents a different graph instance with different available reactions.", "paragraphs": [ "Cellular membranes make metabolism geographically constrained. Cytosolic NADH does not simply become matrix NADH; shuttle systems transfer reducing equivalents through coupled reactions and transport steps. Long-chain acyl groups cross the inner mitochondrial membrane through carnitine-linked chemistry rather than by moving long-chain acyl-CoA unchanged.", "The release model therefore keys a compound instance by both molecular identity and Reactome compartment. A line crosses a compartment boundary only when the source event places participants on different sides. Visual proximity to a membrane never creates an inferred transporter.", "Some Reactome events are black boxes: the source knows that a transition occurs but does not expand balanced internal chemistry. Those events remain labeled as collapsed objects instead of receiving an invented equation." ], "key_ideas": [ "Chemical identity plus compartment defines an instance.", "Transport requires an explicit source event.", "Shuttles transfer chemical equivalents through coupled steps.", "Black-box events remain visibly collapsed." ], "pathway_ids": [ "malate-aspartate-shuttle", "carnitine-shuttle", "inositol-phosphate" ], "source_links": [ { "source": "Reactome malate–aspartate shuttle", "id": "R-HSA-9856872", "url": "https://reactome.org/content/detail/R-HSA-9856872" }, { "source": "Reactome carnitine shuttle", "id": "R-HSA-200425", "url": "https://reactome.org/content/detail/R-HSA-200425" } ], "further_links": null }, { "id": "cofactor-chemistry", "title": "Cofactors as recurring chemical strategies", "kicker": "REACTION CHEMISTRY", "summary": "Vitamin-derived cofactors are best understood by the difficult chemistry they enable across otherwise distant pathways.", "paragraphs": [ "Thiamin diphosphate stabilizes carbon-centered intermediates in oxidative decarboxylation and carbon-transfer reactions. Flavin and nicotinamide cofactors carry electrons through different molecular mechanisms. Coenzyme A activates and transfers acyl groups, while biotin carries activated carbon dioxide in carboxylation reactions.", "Pyridoxal phosphate reorganizes amino-acid bonds through a covalent enzyme-bound intermediate. Tetrahydrofolate carries one-carbon units at several oxidation states, and cobalamin supports radical rearrangement or methyl-transfer chemistry that would be difficult by ordinary ionic mechanisms.", "The Cofactor Lens searches the release-pinned atlas reaction inventory for these molecular forms and their synonyms. Its count describes coverage in this release—not dietary requirement, nutritional status or a claim that every cofactor-dependent human reaction is represented." ], "key_ideas": [ "Cofactors solve recurring chemical problems.", "The active cofactor differs from its dietary precursor.", "A cofactor family can connect distant pathway territories.", "Coverage counts are not nutritional measurements." ], "pathway_ids": [ "water-soluble-vitamins", "cofactor-metabolism", "folate-pterines" ], "source_links": [ { "source": "Reactome vitamins and cofactors", "id": "R-HSA-196854", "url": "https://reactome.org/content/detail/R-HSA-196854" }, { "source": "Reactome cofactor metabolism", "id": "R-HSA-8978934", "url": "https://reactome.org/content/detail/R-HSA-8978934" } ], "further_links": null }, { "id": "oxalosuccinate-focus", "title": "Oxalosuccinate: a reaction intermediate, not a free-standing pool", "kicker": "MECHANISTIC FOCUS", "summary": "The namesake compound is most useful as a lesson in scale: a net pathway arrow can conceal chemically meaningful enzyme-bound steps.", "paragraphs": [ "Mitochondrial NADP-dependent isocitrate dehydrogenase couples oxidation of isocitrate to reduction of NADP+, then decarboxylates the oxidized intermediate. Rhea represents both the overall reaction and stepwise chemistry involving charge-specific (S)-oxalosuccinate.", "The map therefore shows oxalosuccinate inside a mechanistic inset rather than as a large shared metabolite station. That visual choice keeps a transient intermediate from being mistaken for an independently measured cellular pool.", "The NAD-dependent IDH3 route and NADP-dependent IDH2 route remain distinct because their cofactors, enzyme assemblies and physiological contexts are not interchangeable even when their displayed carbon products overlap." ], "key_ideas": [ "Net reactions can conceal ordered steps.", "Charge and stereochemistry are part of chemical identity.", "Shared products do not make cofactor-specific routes equivalent." ], "pathway_ids": [ "tca" ], "source_links": [ { "source": "Reactome", "id": "R-HSA-450984", "url": "https://reactome.org/content/detail/R-HSA-450984" }, { "source": "Rhea", "id": "RHEA:19630", "url": "https://www.rhea-db.org/rhea/19630" }, { "source": "ChEBI", "id": "CHEBI:153066", "url": "https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:153066" } ], "further_links": null } ] }